Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 28 |
| Acc36I | ACCTGC | 1 cut(s) | 28 |
| Acc65I | GGTACC | 1 cut(s) | 1040 |
| AccB1I | GGYRCC | 1 cut(s) | 1040 |
| AccII | CGCG | 1 cut(s) | 1204 |
| AccIII | TCCGGA | 1 cut(s) | 256 |
| AciI | CCGC | 5 cut(s) | 866, 917, 1118, 1202, 1204 |
| AclWI | GGATC | 1 cut(s) | 94 |
| AcsI | RAATTY | 3 cut(s) | 1157, 1182, 1399 |
| AcuI | CTGAAG | 1 cut(s) | 449 |
| AfaI | GTAC | 8 cut(s) | 110, 129, 404, 665, 712, 817, 1042, 1136 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 302, 347, 1051, 1052 |
| AgsI | TTSAA | 4 cut(s) | 123, 670, 705, 977 |
| AjnI | CCWGG | 2 cut(s) | 905, 1394 |
| AleI | CACNNNNGTG | 1 cut(s) | 1353 |
| AluBI | AGCT | 7 cut(s) | 446, 545, 758, 824, 923, 1238, 1286 |
| AluI | AGCT | 7 cut(s) | 446, 545, 758, 824, 923, 1238, 1286 |
| Alw21I | GWGCWC | 1 cut(s) | 1365 |
| Alw26I | GTCTC | 1 cut(s) | 163 |
| AlwI | GGATC | 1 cut(s) | 94 |
| Aor13HI | TCCGGA | 1 cut(s) | 256 |
| AoxI | GGCC | 3 cut(s) | 167, 261, 1205 |
| ApeKI | GCWGC | 5 cut(s) | 197, 920, 923, 963, 1322 |
| ApoI | RAATTY | 3 cut(s) | 1157, 1182, 1399 |
| Asp718I | GGTACC | 1 cut(s) | 1040 |
| AspS9I | GGNCC | 4 cut(s) | 167, 203, 338, 849 |
| AsuHPI | GGTGA | 3 cut(s) | 9, 379, 397 |
| AvaII | GGWCC | 3 cut(s) | 203, 338, 849 |
| BaeGI | GKGCMC | 2 cut(s) | 287, 647 |
| BanI | GGYRCC | 1 cut(s) | 1040 |
| BbsI | GAAGAC | 1 cut(s) | 189 |
| Bbv12I | GWGCWC | 1 cut(s) | 1365 |
| BbvI | GCAGC | 5 cut(s) | 209, 910, 932, 975, 1309 |
| BccI | CCATC | 2 cut(s) | 848, 1374 |
| BciT130I | CCWGG | 2 cut(s) | 907, 1396 |
| BciVI | GTATCC | 1 cut(s) | 785 |
| BcoDI | GTCTC | 1 cut(s) | 163 |
| BfaI | CTAG | 3 cut(s) | 554, 738, 914 |
| BfmI | CTRYAG | 2 cut(s) | 273, 514 |
| BfuAI | ACCTGC | 1 cut(s) | 28 |
| BfuI | GTATCC | 1 cut(s) | 785 |
| BisI | GCNGC | 8 cut(s) | 198, 918, 921, 924, 964, 1119, 1205, 1323 |
| BlsI | GCNGC | 8 cut(s) | 199, 919, 922, 925, 965, 1120, 1206, 1324 |
| BmcAI | AGTACT | 1 cut(s) | 712 |
| Bme1390I | CCNGG | 2 cut(s) | 907, 1396 |
| Bme18I | GGWCC | 3 cut(s) | 203, 338, 849 |
| BmgT120I | GGNCC | 4 cut(s) | 167, 203, 338, 849 |
| BmiI | GGNNCC | 4 cut(s) | 168, 205, 339, 1042 |
| BmrFI | CCNGG | 2 cut(s) | 907, 1396 |
| BmrI | ACTGGG | 1 cut(s) | 706 |
| BmsI | GCATC | 1 cut(s) | 993 |
| BmuI | ACTGGG | 1 cut(s) | 706 |
| BpiI | GAAGAC | 1 cut(s) | 189 |
| BpmI | CTGGAG | 2 cut(s) | 840, 1378 |
| BpuEI | CTTGAG | 1 cut(s) | 1283 |
| BsaI | GGTCTC | 1 cut(s) | 163 |
| BsaJI | CCNNGG | 3 cut(s) | 464, 906, 1202 |
| BsaWI | WCCGGW | 2 cut(s) | 256, 1073 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 302, 347, 1051, 1052 |
| Bse118I | RCCGGY | 1 cut(s) | 200 |
| Bse1I | ACTGG | 4 cut(s) | 701, 817, 823, 830 |
| Bse3DI | GCAATG | 1 cut(s) | 1317 |
| BseAI | TCCGGA | 1 cut(s) | 256 |
| BseBI | CCWGG | 2 cut(s) | 907, 1396 |
| BseDI | CCNNGG | 3 cut(s) | 464, 906, 1202 |
| BseGI | GGATG | 2 cut(s) | 1008, 1152 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 302, 347, 1051, 1052 |
| BseMI | GCAATG | 1 cut(s) | 1317 |
| BseNI | ACTGG | 4 cut(s) | 701, 817, 823, 830 |
| BseSI | GKGCMC | 2 cut(s) | 287, 647 |
| BseXI | GCAGC | 5 cut(s) | 209, 910, 932, 975, 1309 |
| BseYI | CCCAGC | 2 cut(s) | 537, 883 |
| BsgI | GTGCAG | 1 cut(s) | 212 |
| Bsh1236I | CGCG | 1 cut(s) | 1204 |
| BshFI | GGCC | 3 cut(s) | 169, 263, 1207 |
| BshNI | GGYRCC | 1 cut(s) | 1040 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1365 |
| BsiSI | CCGG | 3 cut(s) | 201, 257, 1074 |
| BslFI | GGGAC | 2 cut(s) | 189, 1240 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 302, 347, 1051, 1052 |
| BsmAI | GTCTC | 1 cut(s) | 163 |
| BsmFI | GGGAC | 2 cut(s) | 189, 1240 |
| BsmI | GAATGC | 2 cut(s) | 1013, 1166 |
| BsnI | GGCC | 3 cut(s) | 169, 263, 1207 |
| Bso31I | GGTCTC | 1 cut(s) | 163 |
| Bsp1286I | GDGCHC | 3 cut(s) | 287, 647, 1365 |
| Bsp13I | TCCGGA | 1 cut(s) | 256 |
| Bsp143I | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| BspACI | CCGC | 5 cut(s) | 866, 917, 1118, 1202, 1204 |
| BspANI | GGCC | 3 cut(s) | 169, 263, 1207 |
| BspEI | TCCGGA | 1 cut(s) | 256 |
| BspFNI | CGCG | 1 cut(s) | 1204 |
| BspLI | GGNNCC | 4 cut(s) | 168, 205, 339, 1042 |
| BspMAI | CTGCAG | 1 cut(s) | 277 |
| BspMI | ACCTGC | 1 cut(s) | 28 |
| BspPI | GGATC | 1 cut(s) | 94 |
| BspT107I | GGYRCC | 1 cut(s) | 1040 |
| BspTNI | GGTCTC | 1 cut(s) | 163 |
| BsrDI | GCAATG | 1 cut(s) | 1317 |
| BsrFI | RCCGGY | 1 cut(s) | 200 |
| BsrI | ACTGG | 4 cut(s) | 701, 817, 823, 830 |
| BssAI | RCCGGY | 1 cut(s) | 200 |
| BssECI | CCNNGG | 3 cut(s) | 464, 906, 1202 |
| BssMI | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| BssT1I | CCWWGG | 1 cut(s) | 464 |
| Bst2UI | CCWGG | 2 cut(s) | 907, 1396 |
| Bst4CI | ACNGT | 4 cut(s) | 188, 649, 1139, 1264 |
| Bst6I | CTCTTC | 3 cut(s) | 753, 810, 1370 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1019 |
| BstC8I | GCNNGC | 1 cut(s) | 195 |
| BstDEI | CTNAG | 2 cut(s) | 377, 606 |
| BstDSI | CCRYGG | 1 cut(s) | 1202 |
| BstF5I | GGATG | 2 cut(s) | 1008, 1152 |
| BstFNI | CGCG | 1 cut(s) | 1204 |
| BstKTI | GATC | 5 cut(s) | 89, 147, 838, 1031, 1305 |
| BstMAI | GTCTC | 1 cut(s) | 163 |
| BstMBI | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 443, 872, 923, 1019 |
| BstNI | CCWGG | 2 cut(s) | 907, 1396 |
| BstSCI | CCNGG | 2 cut(s) | 905, 1394 |
| BstSFI | CTRYAG | 2 cut(s) | 273, 514 |
| BstSLI | GKGCMC | 2 cut(s) | 287, 647 |
| BstUI | CGCG | 1 cut(s) | 1204 |
| BstV1I | GCAGC | 5 cut(s) | 209, 910, 932, 975, 1309 |
| BstV2I | GAAGAC | 1 cut(s) | 189 |
| BstX2I | RGATCY | 1 cut(s) | 86 |
| BstYI | RGATCY | 1 cut(s) | 86 |
| BsuI | GTATCC | 1 cut(s) | 785 |
| BsuRI | GGCC | 3 cut(s) | 169, 263, 1207 |
| BtgI | CCRYGG | 1 cut(s) | 1202 |
| BtsCI | GGATG | 2 cut(s) | 1008, 1152 |
| BtsI | GCAGTG | 1 cut(s) | 483 |
| BtsIMutI | CAGTG | 5 cut(s) | 193, 483, 645, 1269, 1437 |
| BveI | ACCTGC | 1 cut(s) | 28 |
| Cac8I | GCNNGC | 1 cut(s) | 195 |
| Cfr10I | RCCGGY | 1 cut(s) | 200 |
| Cfr13I | GGNCC | 4 cut(s) | 167, 203, 338, 849 |
| Cfr42I | CCGCGG | 1 cut(s) | 1205 |
| Csp6I | GTAC | 8 cut(s) | 109, 128, 403, 664, 711, 816, 1041, 1135 |
| CviAII | CATG | 5 cut(s) | 586, 778, 894, 937, 1306 |
| CviQI | GTAC | 8 cut(s) | 109, 128, 403, 664, 711, 816, 1041, 1135 |
| DdeI | CTNAG | 2 cut(s) | 377, 606 |
| DpnI | GATC | 5 cut(s) | 88, 146, 837, 1030, 1304 |
| DpnII | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| Eam1104I | CTCTTC | 3 cut(s) | 753, 810, 1370 |
| EarI | CTCTTC | 3 cut(s) | 753, 810, 1370 |
| Eco130I | CCWWGG | 1 cut(s) | 464 |
| Eco147I | AGGCCT | 1 cut(s) | 263 |
| Eco31I | GGTCTC | 1 cut(s) | 163 |
| Eco47I | GGWCC | 3 cut(s) | 203, 338, 849 |
| Eco57I | CTGAAG | 1 cut(s) | 449 |
| EcoRI | GAATTC | 3 cut(s) | 1157, 1182, 1399 |
| EcoRII | CCWGG | 2 cut(s) | 905, 1394 |
| EcoT14I | CCWWGG | 1 cut(s) | 464 |
| EcoT22I | ATGCAT | 1 cut(s) | 1429 |
| ErhI | CCWWGG | 1 cut(s) | 464 |
| FaeI | CATG | 5 cut(s) | 589, 781, 897, 940, 1309 |
| FaqI | GGGAC | 2 cut(s) | 189, 1240 |
| FatI | CATG | 5 cut(s) | 585, 777, 893, 936, 1305 |
| FauNDI | CATATG | 1 cut(s) | 245 |
| Fnu4HI | GCNGC | 8 cut(s) | 198, 918, 921, 924, 964, 1119, 1205, 1323 |
| FokI | GGATG | 2 cut(s) | 1015, 1159 |
| Fsp4HI | GCNGC | 8 cut(s) | 198, 918, 921, 924, 964, 1119, 1205, 1323 |
| FspBI | CTAG | 3 cut(s) | 554, 738, 914 |
| GluI | GCNGC | 8 cut(s) | 198, 918, 921, 924, 964, 1119, 1205, 1323 |
| GsaI | CCCAGC | 2 cut(s) | 541, 887 |
| GsuI | CTGGAG | 2 cut(s) | 840, 1378 |
| HaeIII | GGCC | 3 cut(s) | 169, 263, 1207 |
| HapII | CCGG | 3 cut(s) | 201, 257, 1074 |
| Hin1II | CATG | 5 cut(s) | 589, 781, 897, 940, 1309 |
| HinfI | GANTC | 4 cut(s) | 685, 701, 968, 1056 |
| HpaII | CCGG | 3 cut(s) | 201, 257, 1074 |
| HphI | GGTGA | 3 cut(s) | 9, 379, 397 |
| Hpy188I | TCNGA | 6 cut(s) | 429, 510, 810, 853, 1405, 1414 |
| Hpy188III | TCNNGA | 7 cut(s) | 142, 257, 419, 682, 839, 1171, 1300 |
| HpyAV | CCTTC | 3 cut(s) | 77, 227, 627 |
| HpyCH4III | ACNGT | 4 cut(s) | 188, 649, 1139, 1264 |
| HpyCH4V | TGCA | 9 cut(s) | 23, 193, 239, 275, 476, 494, 1013, 1348, 1427 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 443, 872, 923, 1019 |
| HpyF3I | CTNAG | 2 cut(s) | 377, 606 |
| Hsp92II | CATG | 5 cut(s) | 589, 781, 897, 940, 1309 |
| Kpn2I | TCCGGA | 1 cut(s) | 256 |
| KpnI | GGTACC | 1 cut(s) | 1044 |
| KspI | CCGCGG | 1 cut(s) | 1205 |
| Kzo9I | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| LmnI | GCTCC | 2 cut(s) | 821, 1235 |
| Lsp1109I | GCAGC | 5 cut(s) | 209, 910, 932, 975, 1309 |
| LweI | GCATC | 1 cut(s) | 993 |
| MaeI | CTAG | 3 cut(s) | 554, 738, 914 |
| MalI | GATC | 5 cut(s) | 88, 146, 837, 1030, 1304 |
| MboI | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| MboII | GAAGA | 7 cut(s) | 81, 139, 194, 563, 740, 797, 1387 |
| MfeI | CAATTG | 2 cut(s) | 123, 471 |
| MflI | RGATCY | 1 cut(s) | 86 |
| MhlI | GDGCHC | 3 cut(s) | 287, 647, 1365 |
| MlyI | GAGTC | 1 cut(s) | 962 |
| MmeI | TCCRAC | 3 cut(s) | 27, 769, 1283 |
| MnlI | CCTC | 9 cut(s) | 25, 141, 148, 253, 615, 813, 881, 1056, 1266 |
| Mph1103I | ATGCAT | 1 cut(s) | 1429 |
| MroI | TCCGGA | 1 cut(s) | 256 |
| MseI | TTAA | 6 cut(s) | 524, 651, 690, 899, 1178, 1368 |
| MslI | CAYNNNNRTG | 2 cut(s) | 941, 1353 |
| MspA1I | CMGCKG | 2 cut(s) | 923, 1204 |
| MspI | CCGG | 3 cut(s) | 201, 257, 1074 |
| MspR9I | CCNGG | 2 cut(s) | 907, 1396 |
| MunI | CAATTG | 2 cut(s) | 123, 471 |
| Mva1269I | GAATGC | 2 cut(s) | 1013, 1166 |
| MvaI | CCWGG | 2 cut(s) | 907, 1396 |
| MvnI | CGCG | 1 cut(s) | 1204 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 443, 872, 923, 1019 |
| NdeI | CATATG | 1 cut(s) | 245 |
| NdeII | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| NlaIII | CATG | 5 cut(s) | 589, 781, 897, 940, 1309 |
| NlaIV | GGNNCC | 4 cut(s) | 168, 205, 339, 1042 |
| NsiI | ATGCAT | 1 cut(s) | 1429 |
| OliI | CACNNNNGTG | 1 cut(s) | 1353 |
| PaqCI | CACCTGC | 1 cut(s) | 28 |
| PceI | AGGCCT | 1 cut(s) | 263 |
| PctI | GAATGC | 2 cut(s) | 1013, 1166 |
| PfeI | GAWTC | 3 cut(s) | 685, 701, 1056 |
| PfoI | TCCNGGA | 1 cut(s) | 1394 |
| PkrI | GCNGC | 8 cut(s) | 199, 919, 922, 925, 965, 1120, 1206, 1324 |
| PleI | GAGTC | 1 cut(s) | 962 |
| PpsI | GAGTC | 1 cut(s) | 962 |
| Psp6I | CCWGG | 2 cut(s) | 905, 1394 |
| PspFI | CCCAGC | 2 cut(s) | 537, 883 |
| PspGI | CCWGG | 2 cut(s) | 905, 1394 |
| PspN4I | GGNNCC | 4 cut(s) | 168, 205, 339, 1042 |
| PspPI | GGNCC | 4 cut(s) | 167, 203, 338, 849 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1118, 1150 |
| PstI | CTGCAG | 1 cut(s) | 277 |
| PsuI | RGATCY | 1 cut(s) | 86 |
| PvuII | CAGCTG | 1 cut(s) | 923 |
| RsaI | GTAC | 8 cut(s) | 110, 129, 404, 665, 712, 817, 1042, 1136 |
| RsaNI | GTAC | 8 cut(s) | 109, 128, 403, 664, 711, 816, 1041, 1135 |
| RseI | CAYNNNNRTG | 2 cut(s) | 941, 1353 |
| SacII | CCGCGG | 1 cut(s) | 1205 |
| SaqAI | TTAA | 6 cut(s) | 524, 651, 690, 899, 1178, 1368 |
| SatI | GCNGC | 8 cut(s) | 198, 918, 921, 924, 964, 1119, 1205, 1323 |
| Sau3AI | GATC | 5 cut(s) | 86, 144, 835, 1028, 1302 |
| Sau96I | GGNCC | 4 cut(s) | 167, 203, 338, 849 |
| ScaI | AGTACT | 1 cut(s) | 712 |
| SchI | GAGTC | 1 cut(s) | 962 |
| ScrFI | CCNGG | 2 cut(s) | 907, 1396 |
| SduI | GDGCHC | 3 cut(s) | 287, 647, 1365 |
| SfaNI | GCATC | 1 cut(s) | 993 |
| SfcI | CTRYAG | 2 cut(s) | 273, 514 |
| Sfr303I | CCGCGG | 1 cut(s) | 1205 |
| SgrBI | CCGCGG | 1 cut(s) | 1205 |
| SinI | GGWCC | 3 cut(s) | 203, 338, 849 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 941, 1353 |
| SmlI | CTYRAG | 1 cut(s) | 1298 |
| SmoI | CTYRAG | 1 cut(s) | 1298 |
| SseBI | AGGCCT | 1 cut(s) | 263 |
| SsiI | CCGC | 5 cut(s) | 866, 917, 1118, 1202, 1204 |
| SspMI | CTAG | 3 cut(s) | 554, 738, 914 |
| StuI | AGGCCT | 1 cut(s) | 263 |
| StyD4I | CCNGG | 2 cut(s) | 905, 1394 |
| StyI | CCWWGG | 1 cut(s) | 464 |
| TaaI | ACNGT | 4 cut(s) | 188, 649, 1139, 1264 |
| TaqI | TCGA | 2 cut(s) | 180, 620 |
| TatI | WGTACW | 4 cut(s) | 108, 663, 710, 1134 |
| TauI | GCSGC | 3 cut(s) | 920, 1121, 1207 |
| TfiI | GAWTC | 3 cut(s) | 685, 701, 1056 |
| Tru1I | TTAA | 6 cut(s) | 524, 651, 690, 899, 1178, 1368 |
| Tru9I | TTAA | 6 cut(s) | 524, 651, 690, 899, 1178, 1368 |
| TscAI | CASTG | 5 cut(s) | 193, 483, 652, 1269, 1437 |
| TseI | GCWGC | 5 cut(s) | 197, 920, 923, 963, 1322 |
| TspDTI | ATGAA | 3 cut(s) | 17, 315, 1150 |
| TspRI | CASTG | 5 cut(s) | 193, 483, 652, 1269, 1437 |
| VpaK11BI | GGWCC | 3 cut(s) | 203, 338, 849 |
| XapI | RAATTY | 3 cut(s) | 1157, 1182, 1399 |
| XspI | CTAG | 3 cut(s) | 554, 738, 914 |
| ZrmI | AGTACT | 1 cut(s) | 712 |
| Zsp2I | ATGCAT | 1 cut(s) | 1429 |