Rroxscaffold_1G00040720

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000162
Physical Location & Seq
Forward (+)
58484811 .. 58489235
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040720.1

Sequence Viewer

Length: 2328 bp
ATGGAGAGACAAATTATCTTGCTTGATGAAGGATGGGGCCATATGCAGAATGGGATCACCAAGCTGAAGAGAATTCTCGAAGGACTACCGGAGCCTCAGTTCAGCTCACAGGAATACATAGATCTTTACACAGTGATTTACAACATGTGTGTTCAGAAGCCCCCTCACGACTACTCTGTACAGCTCTATAATAATTACGGCGAGGTCTTCGTCGAGTACATCGAGTCGACGGTGTTGCCTTCCCTGAGAGAGAAGCACGACGAGTTCATGATGCGTGAGCTCGTCCGAAGATGGTCCAATCACAAACTCATGGTCCGGTGGCTCACTCGCTTCTTTCAGTATCTCGACCGCTACTACGTCTGCCGAAACTCCAAGGATCCGCTCAAGGATGCTGGACTTAAGCGATTTAAGGATCACGTTTACAACGAGATGAATGCAAAGGCTAGGGACTCTCTGATAGCTCTTATTTTGAGGGAACGTGAAGGGGAGCAGATTGACAGAGCGCTGATGAAGAACGTGGTTGATATATTCGTGGAGGTTGGAATGGGAGAGATGGTGAATTACGAGGAGGAGTTCGAAAAACACATGGTTGATGATACTGGGGACTACTACTCTCGAAAGGCCTCGATTTGGATTGTGGAGGATTCTTGTCCCGAGTACATGATGAAGGCGGAGGAGGCCTTGAGGAAGGAGAAGGAGAGAGTTTCCCATTACCTACATTCGGGCAGCGAGCAAAAGCTGTTGGAGAGGGTGCAGCAAGAGTTGTTGGTGGTTCAAGGTCCACAGCTGCTTGATAAAGAGGGGTCTGGATGCCGGACCTTGCTTCGAGACGATAGGGTGGATGACTTGTCTAGGATGTTCAGGCTTTACCATAAAATACCCCAAGGGTTGGATCCTGTTGCTGCTATGTTTAAGAAGCATGTTACTAATGAAGGCACAGCCTTGGTCCAACAGGCCGAAGAGGCAACAGCAGCTGCAAGTGGCAACAAGCAAGGTGGTGGTGCTGGTGAGCCGGGACTAGTATTTATCAGCAAAATACTTGAGCTACATGATAAGTACATGGGATATGTCCTTAGCTGCTTCCAAAACCACTCGCTCTTCCACAAGGCCTTGAAAGAGGCTTTTGAGGTATTCTGTAATAAATCTGTTGCTGGGTGCTCGAGTGCAGAATTGCTTGCTACATTCTGTGATAGTATCCTCAAGCAGATGGGTGAGAGTGACGAGGCCAAAGAAGAAACTCTTGACAAGATTGTTACGGTTCTTGCATATTTCAGTGACAAGGATCTTTTCGCCGAATTCTGCAGGAAGAAACTTGCCCGTAGGCTGCTCATGGTTCATGATGATAAGAAGAACCGTGCTGACCAGCTTGACAATGAGAGAATGCTTCTAACAAAGCTGAAACAGCAATGTGGAGGACAGTTCACCTCTAAGATGGAGGGAATGGTCACAGATATTACTATTTCTCGGGAAAGCGAGAAGAGTTTCAAAGACTATCTTCGAAAAGATGGTACTCCAAAGCTTGAGTTGGATTTCTCAGTCACTGTTCTAACAACTGGTAACTGGCCAAGCTATAAAACATCTGATCTTCACCTTCCTGAAGAGATGGTCAAGTGCATTGAAGTGTATAAAGAATATTATGACAGGGAAAAGAAACACAGGAAGCTTTCATGGATTTATTCATTGGGTACTTGCCATGTTAGGGGCAACTTTGAGCCAAAACCCATTGAAATGCTTATGTCAACCTATCAGGCTGCTCTCCTGCTACTCTTCAACACCTCGGAGAAATTGAGCTTTTCAGAAATATTGACTCAATTAAATCTTACCAAAGATGATGTCCACAGAACGCTTCATTCATTTTCTTGTGCCAAATTGAGGAAAATCAGGATCCCTCTGCCACCGCTGCAGGATGAAAGGAAGAAGGTCACGGAAGATGTCGACAAAGAGCGCAAGTATACAATTGACGCTGCACTGGTGAGGATTATGAAGAGTCGAAAAATATTGGGTCATCAACAACTGGTCACTGAGTGTCTTGAACAGTTGCAGCGCACGTTCAAGCCAGACATCAAAACAATCAAGAGGCGCATTGAAGATCTCATCACCCGTGACTACCTTGAGAGGGACCCTGAGAATCCTAACACATTCAACCTATCAACGCGCCCTGGATCTCAACCTCGACCACCTCCTCTCCCGGCGCTCCGACCTCGACAAGCACATCTCCAATCTCCGAAATCCTCCCAGGTCCTCGACATCGTCAAGTCCAACGCCTCCTCCACCTGCGACCTCGCCGACCACGTCGGCACCAAGGTCCGCGAGCTCGACCTCGCCTAA

Protein Analysis

775

Amino Acids

89.84

Weight (kDa)

6.91

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 25 - 490 5.6e-120 Cullin alpha solenoid domain
Cullin_AB PF26557 515 - 653 2.3e-34 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 648 - 710 2.4e-26 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2282
Acc36I ACCTGC 1 cut(s) 2282
AccB1I GGYRCC 1 cut(s) 2297
AccB7I CCANNNNNTGG 1 cut(s) 889
AccBSI CCGCTC 1 cut(s) 382
AccI GTMKAC 3 cut(s) 227, 1935, 1952
AccII CGCG 2 cut(s) 2155, 2310
AciI CCGC 5 cut(s) 349, 380, 671, 1898, 2308
AcoI YGGCCR 1 cut(s) 1562
AcsI RAATTY 2 cut(s) 72, 1295
AcuI CTGAAG 2 cut(s) 86, 1617
AdeI CACNNNGTG 1 cut(s) 2025
AfaI GTAC 6 cut(s) 180, 218, 659, 1058, 1510, 1687
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 6 cut(s) 630, 721, 889, 1699, 1872, 2116
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 144
AhlI ACTAGT 1 cut(s) 1018
AjiI CACGTC 1 cut(s) 2293
AjnI CCWGG 2 cut(s) 2158, 2235
AloI GAACNNNNNNTCC 2 cut(s) 83, 115
Alw21I GWGCWC 3 cut(s) 282, 1160, 2316
Alw26I GTCTC 1 cut(s) 822
AlwNI CAGNNNCTG 2 cut(s) 974, 1541
Ama87I CYCGRG 3 cut(s) 653, 1159, 1464
Aor51HI AGCGCT 1 cut(s) 504
AoxI GGCC 7 cut(s) 37, 621, 678, 954, 1107, 1224, 1562
ApoI RAATTY 2 cut(s) 72, 1295
ArsI GACNNNNNNTTYG 4 cut(s) 297, 329, 1818, 1850
Asp700I GAANNNNTTC 1 cut(s) 1481
AspLEI GCGC 6 cut(s) 505, 1947, 2046, 2082, 2157, 2194
AspS9I GGNCC 9 cut(s) 37, 294, 313, 779, 816, 946, 2119, 2239, 2305
AsuC2I CCSGG 2 cut(s) 1014, 2189
AsuHPI GGTGA 8 cut(s) 49, 568, 1019, 1223, 1414, 1580, 1984, 2089
AsuII TTCGAA 2 cut(s) 576, 1498
AvaI CYCGRG 3 cut(s) 653, 1159, 1464
AvaII GGWCC 8 cut(s) 294, 313, 779, 816, 946, 2119, 2239, 2305
BalI TGGCCA 1 cut(s) 1564
BamHI GGATCC 3 cut(s) 376, 892, 1884
BanI GGYRCC 1 cut(s) 2297
BanII GRGCYC 2 cut(s) 282, 2316
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 3 cut(s) 282, 1160, 2316
BccI CCATC 7 cut(s) 27, 285, 547, 1201, 1426, 1499, 1597
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 4 cut(s) 217, 251, 416, 450
BciT130I CCWGG 2 cut(s) 2160, 2237
BciVI GTATCC 1 cut(s) 1205
BcnI CCSGG 2 cut(s) 1014, 2189
BcoDI GTCTC 1 cut(s) 822
BcuI ACTAGT 1 cut(s) 1018
BfaI CTAG 3 cut(s) 444, 852, 1019
BfmI CTRYAG 2 cut(s) 1300, 1901
BfoI RGCGCY 2 cut(s) 506, 2195
BfrI CTTAAG 1 cut(s) 398
BfuAI ACCTGC 1 cut(s) 2282
BfuI GTATCC 1 cut(s) 1205
BglI GCCNNNNNGGC 1 cut(s) 962
BglII AGATCT 2 cut(s) 121, 2089
Bme1390I CCNGG 4 cut(s) 1014, 2160, 2189, 2237
Bme18I GGWCC 8 cut(s) 294, 313, 779, 816, 946, 2119, 2239, 2305
BmeT110I CYCGRG 3 cut(s) 653, 1159, 1464
BmgBI CACGTC 1 cut(s) 2293
BmgT120I GGNCC 9 cut(s) 37, 294, 313, 779, 816, 946, 2119, 2239, 2305
BmiI GGNNCC 8 cut(s) 38, 93, 378, 894, 1886, 2120, 2121, 2299
BmrFI CCNGG 4 cut(s) 1014, 2160, 2189, 2237
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 3 cut(s) 261, 379, 800
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 1 cut(s) 199
Bpu10I CCTNAGC 1 cut(s) 1073
Bpu14I TTCGAA 2 cut(s) 576, 1498
BpuEI CTTGAG 6 cut(s) 368, 703, 1061, 1184, 1541, 2132
BpuMI CCSGG 2 cut(s) 1014, 2189
BsaJI CCNNGG 7 cut(s) 372, 883, 942, 1776, 2158, 2235, 2301
BsaWI WCCGGW 2 cut(s) 88, 315
BsaXI ACNNNNNCTCC 8 cut(s) 83, 113, 540, 570, 2169, 2199, 2252, 2282
Bsc4I CCNNNNNNNGG 6 cut(s) 630, 721, 889, 1699, 1872, 2116
Bse1I ACTGG 5 cut(s) 604, 1558, 1565, 1974, 2019
Bse3DI GCAATG 1 cut(s) 1412
BseBI CCWGG 2 cut(s) 2160, 2237
BseDI CCNNGG 7 cut(s) 372, 883, 942, 1776, 2158, 2235, 2301
BseGI GGATG 6 cut(s) 38, 394, 815, 847, 861, 1912
BseLI CCNNNNNNNGG 6 cut(s) 630, 721, 889, 1699, 1872, 2116
BseMI GCAATG 1 cut(s) 1412
BseMII CTCAG 5 cut(s) 110, 236, 1548, 2013, 2115
BseNI ACTGG 5 cut(s) 604, 1558, 1565, 1974, 2019
BseRI GAGGAG 5 cut(s) 581, 584, 689, 2172, 2257
BseYI CCCAGC 1 cut(s) 1151
BsgI GTGCAG 3 cut(s) 773, 1185, 1950
Bsh1236I CGCG 2 cut(s) 2155, 2310
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BshNI GGYRCC 1 cut(s) 2297
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 3 cut(s) 282, 1160, 2316
BsiHKCI CYCGRG 3 cut(s) 653, 1159, 1464
BsiSI CCGG 5 cut(s) 89, 316, 814, 1013, 2189
BslFI GGGAC 5 cut(s) 461, 617, 636, 1029, 2132
BslI CCNNNNNNNGG 6 cut(s) 630, 721, 889, 1699, 1872, 2116
BsmAI GTCTC 1 cut(s) 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 5 cut(s) 461, 617, 636, 1029, 2132
BsmI GAATGC 2 cut(s) 439, 1386
BsnI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BsoBI CYCGRG 3 cut(s) 653, 1159, 1464
Bsp119I TTCGAA 2 cut(s) 576, 1498
Bsp1286I GDGCHC 3 cut(s) 282, 1160, 2316
Bsp1407I TGTACA 1 cut(s) 178
BspACI CCGC 5 cut(s) 349, 380, 671, 1898, 2308
BspANI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BspCNI CTCAG 5 cut(s) 109, 237, 1547, 2014, 2116
BspFNI CGCG 2 cut(s) 2155, 2310
BspHI TCATGA 2 cut(s) 267, 1336
BspLI GGNNCC 8 cut(s) 38, 93, 378, 894, 1886, 2120, 2121, 2299
BspMAI CTGCAG 2 cut(s) 1304, 1905
BspMI ACCTGC 1 cut(s) 2282
BspQI GCTCTTC 1 cut(s) 1103
BspT104I TTCGAA 2 cut(s) 576, 1498
BspT107I GGYRCC 1 cut(s) 2297
BspTI CTTAAG 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 1412
BsrGI TGTACA 1 cut(s) 178
BsrI ACTGG 5 cut(s) 604, 1558, 1565, 1974, 2019
BssECI CCNNGG 7 cut(s) 372, 883, 942, 1776, 2158, 2235, 2301
BssNAI GTATAC 1 cut(s) 1953
BssT1I CCWWGG 4 cut(s) 372, 883, 942, 2301
Bst1107I GTATAC 1 cut(s) 1953
Bst2UI CCWGG 2 cut(s) 2160, 2237
Bst4CI ACNGT 7 cut(s) 133, 232, 1258, 1355, 1419, 1543, 2037
Bst6I CTCTTC 7 cut(s) 62, 954, 1103, 1472, 1593, 1772, 1979
BstAFI CTTAAG 1 cut(s) 398
BstAUI TGTACA 1 cut(s) 178
BstBI TTCGAA 2 cut(s) 576, 1498
BstC8I GCNNGC 3 cut(s) 731, 1176, 2312
BstDEI CTNAG 7 cut(s) 96, 245, 1073, 1428, 1534, 2022, 2124
BstF5I GGATG 6 cut(s) 38, 394, 815, 847, 861, 1912
BstFNI CGCG 2 cut(s) 2155, 2310
BstH2I RGCGCY 2 cut(s) 506, 2195
BstHHI GCGC 6 cut(s) 505, 1947, 2046, 2082, 2157, 2194
BstMAI GTCTC 1 cut(s) 822
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1402, 1900
BstNI CCWGG 2 cut(s) 2160, 2237
BstNSI RCATGY 2 cut(s) 148, 923
BstSCI CCNGG 4 cut(s) 1012, 2158, 2187, 2235
BstSFI CTRYAG 2 cut(s) 1300, 1901
BstUI CGCG 2 cut(s) 2155, 2310
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 7 cut(s) 121, 376, 892, 1282, 1884, 2089, 2162
BstYI RGATCY 7 cut(s) 121, 376, 892, 1282, 1884, 2089, 2162
BstZ17I GTATAC 1 cut(s) 1953
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
BtrI CACGTC 1 cut(s) 2293
BtsCI GGATG 6 cut(s) 38, 394, 815, 847, 861, 1912
BtsIMutI CAGTG 5 cut(s) 138, 1279, 1539, 1967, 2019
BveI ACCTGC 1 cut(s) 2282
Cac8I GCNNGC 3 cut(s) 731, 1176, 2312
CaiI CAGNNNCTG 2 cut(s) 974, 1541
CciI TCATGA 2 cut(s) 267, 1336
CfoI GCGC 6 cut(s) 505, 1947, 2046, 2082, 2157, 2194
Cfr13I GGNCC 9 cut(s) 37, 294, 313, 779, 816, 946, 2119, 2239, 2305
CseI GACGC 1 cut(s) 1970
Csp6I GTAC 6 cut(s) 179, 217, 658, 1057, 1509, 1686
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 6 cut(s) 179, 217, 658, 1057, 1509, 1686
DdeI CTNAG 7 cut(s) 96, 245, 1073, 1428, 1534, 2022, 2124
DraIII CACNNNGTG 1 cut(s) 2025
EaeI YGGCCR 1 cut(s) 1562
Eam1104I CTCTTC 7 cut(s) 62, 954, 1103, 1472, 1593, 1772, 1979
EarI CTCTTC 7 cut(s) 62, 954, 1103, 1472, 1593, 1772, 1979
EciI GGCGGA 1 cut(s) 686
Ecl136II GAGCTC 2 cut(s) 280, 2314
Eco130I CCWWGG 4 cut(s) 372, 883, 942, 2301
Eco147I AGGCCT 3 cut(s) 623, 680, 1109
Eco24I GRGCYC 2 cut(s) 282, 2316
Eco47I GGWCC 8 cut(s) 294, 313, 779, 816, 946, 2119, 2239, 2305
Eco47III AGCGCT 1 cut(s) 504
Eco53kI GAGCTC 2 cut(s) 280, 2314
Eco57I CTGAAG 2 cut(s) 86, 1617
Eco88I CYCGRG 3 cut(s) 653, 1159, 1464
EcoICRI GAGCTC 2 cut(s) 280, 2314
EcoO109I RGGNCCY 2 cut(s) 2119, 2239
EcoRI GAATTC 2 cut(s) 72, 1295
EcoRII CCWGG 2 cut(s) 2158, 2235
EcoT14I CCWWGG 4 cut(s) 372, 883, 942, 2301
EcoT38I GRGCYC 2 cut(s) 282, 2316
ErhI CCWWGG 4 cut(s) 372, 883, 942, 2301
Esp3I CGTCTC 1 cut(s) 822
FalI AAGNNNNNCTT 4 cut(s) 1224, 1256, 1479, 1511
FaqI GGGAC 5 cut(s) 461, 617, 636, 1029, 2132
FauNDI CATATG 1 cut(s) 42
FblI GTMKAC 3 cut(s) 227, 1935, 1952
FokI GGATG 6 cut(s) 45, 401, 822, 854, 868, 1919
FriOI GRGCYC 2 cut(s) 282, 2316
FspBI CTAG 3 cut(s) 444, 852, 1019
GlaI GCGC 6 cut(s) 504, 1946, 2045, 2081, 2156, 2193
GsaI CCCAGC 1 cut(s) 1155
HaeII RGCGCY 2 cut(s) 506, 2195
HaeIII GGCC 7 cut(s) 39, 623, 680, 956, 1109, 1226, 1564
HapII CCGG 5 cut(s) 89, 316, 814, 1013, 2189
HgaI GACGC 1 cut(s) 1970
HhaI GCGC 6 cut(s) 505, 1947, 2046, 2082, 2157, 2194
Hin6I GCGC 6 cut(s) 503, 1945, 2044, 2080, 2155, 2192
HinP1I GCGC 6 cut(s) 503, 1945, 2044, 2080, 2155, 2192
HincII GTYRAC 3 cut(s) 228, 1740, 1936
HindII GTYRAC 3 cut(s) 228, 1740, 1936
HindIII AAGCTT 2 cut(s) 1517, 1661
HinfI GANTC 6 cut(s) 224, 449, 644, 1807, 1987, 2128
HpaII CCGG 5 cut(s) 89, 316, 814, 1013, 2189
HphI GGTGA 8 cut(s) 49, 568, 1019, 1223, 1414, 1580, 1984, 2089
Hpy166II GTNNAC 8 cut(s) 228, 421, 782, 1422, 1740, 1837, 1936, 1953
Hpy188I TCNGA 8 cut(s) 156, 287, 456, 1582, 1780, 1798, 2198, 2226
Hpy8I GTNNAC 8 cut(s) 228, 421, 782, 1422, 1740, 1837, 1936, 1953
Hpy99I CGWCG 4 cut(s) 215, 232, 263, 2297
HpyCH4III ACNGT 7 cut(s) 133, 232, 1258, 1355, 1419, 1543, 2037
HpyCH4IV ACGT 6 cut(s) 357, 417, 478, 516, 2048, 2292
HpyF10VI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1402, 1900
HpyF3I CTNAG 7 cut(s) 96, 245, 1073, 1428, 1534, 2022, 2124
HpySE526I ACGT 6 cut(s) 357, 417, 478, 516, 2048, 2292
HspAI GCGC 6 cut(s) 503, 1945, 2044, 2080, 2155, 2192
KflI GGGWCCC 1 cut(s) 2119
LguI GCTCTTC 1 cut(s) 1103
LmnI GCTCC 3 cut(s) 91, 487, 2199
LweI GCATC 3 cut(s) 261, 379, 800
MaeI CTAG 3 cut(s) 444, 852, 1019
MaeII ACGT 6 cut(s) 357, 417, 478, 516, 2048, 2292
MbiI CCGCTC 1 cut(s) 382
MfeI CAATTG 1 cut(s) 1956
MflI RGATCY 7 cut(s) 121, 376, 892, 1282, 1884, 2089, 2162
MhlI GDGCHC 3 cut(s) 282, 1160, 2316
MlsI TGGCCA 1 cut(s) 1564
MluNI TGGCCA 1 cut(s) 1564
MlyI GAGTC 4 cut(s) 233, 443, 1801, 1996
MmeI TCCRAC 7 cut(s) 520, 723, 870, 973, 1506, 2221, 2283
Mox20I TGGCCA 1 cut(s) 1564
MroXI GAANNNNTTC 1 cut(s) 1481
MscI TGGCCA 1 cut(s) 1564
MseI TTAA 4 cut(s) 399, 408, 912, 1814
MslI CAYNNNNRTG 2 cut(s) 1619, 1727
Msp20I TGGCCA 1 cut(s) 1564
MspA1I CMGCKG 3 cut(s) 787, 974, 1900
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 5 cut(s) 89, 316, 814, 1013, 2189
MspR9I CCNGG 4 cut(s) 1014, 2160, 2189, 2237
MunI CAATTG 1 cut(s) 1956
Mva1269I GAATGC 2 cut(s) 439, 1386
MvaI CCWGG 2 cut(s) 2160, 2237
MvnI CGCG 2 cut(s) 2155, 2310
MwoI GCNNNNNNNGC 5 cut(s) 677, 962, 971, 1402, 1900
NciI CCSGG 2 cut(s) 1014, 2189
NdeI CATATG 1 cut(s) 42
NlaIV GGNNCC 8 cut(s) 38, 93, 378, 894, 1886, 2120, 2121, 2299
NmuCI GTSAC 7 cut(s) 1217, 1274, 1444, 1537, 1921, 2017, 2102
NspI RCATGY 2 cut(s) 148, 923
NspV TTCGAA 2 cut(s) 576, 1498
PaeR7I CTCGAG 1 cut(s) 1159
PagI TCATGA 2 cut(s) 267, 1336
PaqCI CACCTGC 1 cut(s) 2282
PceI AGGCCT 3 cut(s) 623, 680, 1109
PciI ACATGT 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 1103
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 423, 2289
PctI GAATGC 2 cut(s) 439, 1386
PdmI GAANNNNTTC 1 cut(s) 1481
PfeI GAWTC 2 cut(s) 644, 2128
PflFI GACNNNGTC 2 cut(s) 2249, 2291
PflMI CCANNNNNTGG 1 cut(s) 889
PleI GAGTC 4 cut(s) 232, 443, 1801, 1995
PpsI GAGTC 4 cut(s) 232, 443, 1801, 1995
PpuMI RGGWCCY 2 cut(s) 2119, 2239
PscI ACATGT 1 cut(s) 144
Psp124BI GAGCTC 2 cut(s) 282, 2316
Psp5II RGGWCCY 2 cut(s) 2119, 2239
Psp6I CCWGG 2 cut(s) 2158, 2235
PspFI CCCAGC 1 cut(s) 1151
PspGI CCWGG 2 cut(s) 2158, 2235
PspN4I GGNNCC 8 cut(s) 38, 93, 378, 894, 1886, 2120, 2121, 2299
PspPI GGNCC 9 cut(s) 37, 294, 313, 779, 816, 946, 2119, 2239, 2305
PspPPI RGGWCCY 2 cut(s) 2119, 2239
PspXI VCTCGAGB 1 cut(s) 1159
PstI CTGCAG 2 cut(s) 1304, 1905
PstNI CAGNNNCTG 2 cut(s) 974, 1541
PsuI RGATCY 7 cut(s) 121, 376, 892, 1282, 1884, 2089, 2162
PsyI GACNNNGTC 2 cut(s) 2249, 2291
PvuII CAGCTG 2 cut(s) 787, 974
RsaI GTAC 6 cut(s) 180, 218, 659, 1058, 1510, 1687
RsaNI GTAC 6 cut(s) 179, 217, 658, 1057, 1509, 1686
RseI CAYNNNNRTG 2 cut(s) 1619, 1727
SacI GAGCTC 2 cut(s) 282, 2316
SalI GTCGAC 2 cut(s) 226, 1934
SapI GCTCTTC 1 cut(s) 1103
SaqAI TTAA 4 cut(s) 399, 408, 912, 1814
Sau96I GGNCC 9 cut(s) 37, 294, 313, 779, 816, 946, 2119, 2239, 2305
SchI GAGTC 4 cut(s) 233, 443, 1801, 1996
ScrFI CCNGG 4 cut(s) 1014, 2160, 2189, 2237
SduI GDGCHC 3 cut(s) 282, 1160, 2316
SfaNI GCATC 3 cut(s) 261, 379, 800
SfcI CTRYAG 2 cut(s) 1300, 1901
Sfr274I CTCGAG 1 cut(s) 1159
SfuI TTCGAA 2 cut(s) 576, 1498
SinI GGWCC 8 cut(s) 294, 313, 779, 816, 946, 2119, 2239, 2305
SlaI CTCGAG 1 cut(s) 1159
SmiMI CAYNNNNRTG 2 cut(s) 1619, 1727
SmlI CTYRAG 8 cut(s) 383, 398, 682, 1040, 1159, 1199, 1520, 2111
SmoI CTYRAG 8 cut(s) 383, 398, 682, 1040, 1159, 1199, 1520, 2111
SpeI ACTAGT 1 cut(s) 1018
SseBI AGGCCT 3 cut(s) 623, 680, 1109
SsiI CCGC 5 cut(s) 349, 380, 671, 1898, 2308
SspI AATATT 3 cut(s) 1634, 1803, 1998
SspMI CTAG 3 cut(s) 444, 852, 1019
SstI GAGCTC 2 cut(s) 282, 2316
StuI AGGCCT 3 cut(s) 623, 680, 1109
StyD4I CCNGG 4 cut(s) 1012, 2158, 2187, 2235
StyI CCWWGG 4 cut(s) 372, 883, 942, 2301
TaaI ACNGT 7 cut(s) 133, 232, 1258, 1355, 1419, 1543, 2037
TaiI ACGT 6 cut(s) 360, 420, 481, 519, 2051, 2295
TatI WGTACW 4 cut(s) 178, 216, 657, 1056
TfiI GAWTC 2 cut(s) 644, 2128
Tru1I TTAA 4 cut(s) 399, 408, 912, 1814
Tru9I TTAA 4 cut(s) 399, 408, 912, 1814
TscAI CASTG 5 cut(s) 138, 1279, 1546, 1974, 2026
TseFI GTSAC 7 cut(s) 1217, 1274, 1444, 1537, 1921, 2017, 2102
Tsp45I GTSAC 7 cut(s) 1217, 1274, 1444, 1537, 1921, 2017, 2102
TspGWI ACGGA 1 cut(s) 1940
TspRI CASTG 5 cut(s) 138, 1279, 1546, 1974, 2026
Tth111I GACNNNGTC 2 cut(s) 2249, 2291
Van91I CCANNNNNTGG 1 cut(s) 889
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 8 cut(s) 294, 313, 779, 816, 946, 2119, 2239, 2305
XapI RAATTY 2 cut(s) 72, 1295
XceI RCATGY 2 cut(s) 148, 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XhoI CTCGAG 1 cut(s) 1159
XmiI GTMKAC 3 cut(s) 227, 1935, 1952
XmnI GAANNNNTTC 1 cut(s) 1481
XspI CTAG 3 cut(s) 444, 852, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.