MD06G1052800.v1.1

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000162
Physical Location & Seq
Forward (+)
7661051 .. 7664051
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1052800.v1.1.491

Sequence Viewer

Length: 2118 bp
ATGAGTGTGGACTCAGGTAGTCAATTTCGGCCACTTATCGAGTTGGATCAAGGATGGGAATATATATCGAGTATGTTTGCAAAATTAAAGAGGATTTTAGAAGGATTACCGGAGCCTCAGTTCAGTTCCGTTGAATATATGACCCTTTACACAACTGTCTACAATATGTGTACCCAAAAGCCGCCTCACAATTACTCTCAACAGCTTTACGATAAGTATAGGGCGGCGTTGGAGGACTATGTAACCACAACGGTGCTGCCATCTGTAAATGAGAAACACGGCGAGCTTATGCTGCAAGAGCTTGTGAAAAGATGGACGAATTATAAAATTATGATTAGGTGGCTGTCAAATTGTTTTAATTTTCTTGATCGGTTTTTCATCCCTAGGAGATCGCTTCCTAGCCTGAATGATGTTGGAATTACCTGTTTCCGCGATTCGGTTTATCGAAAAGTACAAGTCAATGCTAGAGTTACTGTAATTGGTCTTATTGATAGTGAACGCGTGGGAGAGCAGATTGACAGAGCACTAGTGAAGAATGTGATAGATTTATTTGTTGGAATTGGAATGGGACGAATGGATGCATATGAAGAGGACTTTGAGGCGCACATGCTAAGACATGCTGGCGAATACTATTCTCGTAAAGCAGCAAGTTGGCTTTTGGAGGATTTCTGTCCTGATTTCTTAGTGAAGGCCGAAGAGTGCTTGAGAAGGGAGAGGGATAGAGTTTCTCATTACTTGCATTCAAGCAGTGAATTGAAGTTGGTGGAGAAAGTGCAGCATGAGTTGTTTGAACCGCTTGCTGAAATATTAAAACAGAAAGTTAGTTTTGAAGTAAAATCGTTGGTCCAACAGGCTGAATATACTGCAAGTAACGAGACTTCAGATAGATCTTCTGGCATGCTGGAACAGGTCCTTGTCACAAACATAATCGAGCTTCATGATAAGCATATGGCGTACTTTAATGACTGCTCAATCAAAAACCATGTCTTCCACAAAGCTCTGAGAGAGGCCTTTGATGTATTTTGCAATAAAGCTGTTTCTGGGTATTCAAGTGCTCAATTACTCGCAACATTCTGTGAAAATATCCTCAAAAAGGGTGGGAGTCAGAAGTTGAGTGATGAGGCCATTGAAGTAATGCTTGACAAGGTTGTTAAGTTTCTTACTTATATCAGTGACAAAGACCTTTTTGCAGAATTCTACAGGAAAAAGCTTGCCCGTCGGCTACTTTTTGATCCAAGTGCTGACAAGGAACATGAAAAAAGTATTCTTACAAAGATGAAACAGCAATGTGGTGGCCAGTTCACCTCAAAAATGGAGGGAATGGTCACAGATCTGACATTGGCTCGGGAAAATCAGACTAGCTTTGAGGAGTACCTTTGCAATAGCCCAAATGTAGATCCTGGGATTGATTTTACAATCACCGTTCTTACAACTGGTTTCTGGCCGAGTTATAAAACATGTGATCTTAGCCTTCCTGCAGAGATGGTGAAATGTGTTGAAGTTTTCAAGGGATTCTATGAAAGAAAGACGAAATGCAGAAAAATTACATGGATCTACTCTTTGGGAACTTGCAACATTATTGGAAAGTTCGAGCTAAAAGAAATTGAATTGGTTGTATCGACTTATCAGGGTGCTCTCCTACTGCTTTTCAATAGCATGGATAGGTTGAGCTATTCAGAAATTCTAAGTAAGTTAAACCTTACCCATGAGGCGGCTATCAGGGTTCTTCATTCGCTTTCCTGTGCCAAGTACAAGATCCTTATTAAGGAGCCTGATACAAAAACTATATCACCAAATGACAGCTTTGAGTTCAACTATAAGTTCACCGACAGAATGAGAAGAATTAAGATTCCTCTCCCACCAGTGGAGGAAAGGAAGGAGGTGATCCAAGATGTTGAGAAAGAAAGACGATATGCTATTGATGCAGCAATTGTGCGGATTATGAAGAGCCGGAAAGTTTTGGAACATCAACAGTTAGTTACGGAGTGTGTTGAGCAGTTGAGACATAAGTTCAAGCCTGACATCAGAGCGATTAAGAAGCAGATTGAAGGTCTCATCATCCGTGACTACCTGGAGAGGGACAAGGAAGATCGTAACTTGTTTAAGTATCTTGCGTGA

Protein Analysis

706

Amino Acids

82.08

Weight (kDa)

7.5

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 34 - 282 6.6e-54 Cullin alpha solenoid domain
Cullin PF00888 265 - 450 7.1e-47 Cullin alpha solenoid domain
Cullin_AB PF26557 475 - 608 1.2e-39 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 635 - 697 2.1e-26 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 324, 1452
AccI GTMKAC 1 cut(s) 159
AccII CGCG 2 cut(s) 432, 501
AciI CCGC 6 cut(s) 182, 224, 430, 794, 1712, 1936
AclWI GGATC 6 cut(s) 54, 1226, 1391, 1559, 1750, 1879
AcoI YGGCCR 3 cut(s) 29, 1294, 1442
AcsI RAATTY 2 cut(s) 1193, 1680
AcuI CTGAAG 1 cut(s) 864
AfaI GTAC 5 cut(s) 172, 453, 956, 1373, 1751
AfiI CCNNNNNNNGG 6 cut(s) 436, 1093, 1711, 1765, 1864, 2077
AflIII ACRYGT 2 cut(s) 499, 1457
AhlI ACTAGT 1 cut(s) 526
AjnI CCWGG 2 cut(s) 1399, 2070
AleI CACNNNNGTG 1 cut(s) 251
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
Alw21I GWGCWC 3 cut(s) 526, 1057, 1636
Alw26I GTCTC 3 cut(s) 869, 1996, 2057
AlwI GGATC 6 cut(s) 54, 1226, 1391, 1559, 1750, 1879
Ama87I CYCGRG 1 cut(s) 1344
AoxI GGCC 6 cut(s) 29, 690, 1008, 1122, 1294, 1442
ApeKI GCWGC 5 cut(s) 256, 292, 644, 775, 1925
ApoI RAATTY 2 cut(s) 1193, 1680
ArsI GACNNNNNNTTYG 2 cut(s) 969, 1001
AspA2I CCTAGG 1 cut(s) 383
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 2 cut(s) 844, 910
AsuHPI GGTGA 6 cut(s) 1294, 1411, 1498, 1782, 1816, 1894
AvaI CYCGRG 1 cut(s) 1344
AvaII GGWCC 2 cut(s) 844, 910
AvrII CCTAGG 1 cut(s) 383
BalI TGGCCA 1 cut(s) 1296
BarI GAAGNNNNNNTAC 2 cut(s) 862, 894
BbsI GAAGAC 1 cut(s) 979
Bbv12I GWGCWC 3 cut(s) 526, 1057, 1636
BbvI GCAGC 5 cut(s) 243, 279, 656, 787, 1937
BccI CCATC 4 cut(s) 48, 268, 306, 1477
BceAI ACGGC 1 cut(s) 295
BciT130I CCWGG 2 cut(s) 1401, 2072
BcoDI GTCTC 3 cut(s) 869, 1996, 2057
BcuI ACTAGT 1 cut(s) 526
BfaI CTAG 5 cut(s) 384, 399, 465, 527, 1359
BfmI CTRYAG 2 cut(s) 1198, 1476
BglII AGATCT 2 cut(s) 887, 1330
BisI GCNGC 8 cut(s) 182, 225, 257, 293, 645, 776, 1713, 1926
BlnI CCTAGG 1 cut(s) 383
BlsI GCNGC 8 cut(s) 183, 226, 258, 294, 646, 777, 1714, 1927
Bme1390I CCNGG 2 cut(s) 1401, 2072
Bme18I GGWCC 2 cut(s) 844, 910
BmeT110I CYCGRG 1 cut(s) 1344
BmgT120I GGNCC 2 cut(s) 844, 910
BmiI GGNNCC 2 cut(s) 114, 1770
BmrFI CCNGG 2 cut(s) 1401, 2072
BmsI GCATC 2 cut(s) 568, 1912
BpiI GAAGAC 1 cut(s) 979
BpmI CTGGAG 1 cut(s) 2093
BpuEI CTTGAG 1 cut(s) 724
BsaI GGTCTC 1 cut(s) 2057
BsaJI CCNNGG 2 cut(s) 383, 1400
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 6 cut(s) 436, 1093, 1711, 1765, 1864, 2077
Bse1I ACTGG 3 cut(s) 1297, 1438, 1862
Bse3DI GCAATG 1 cut(s) 1292
BseBI CCWGG 2 cut(s) 1401, 2072
BseDI CCNNGG 2 cut(s) 383, 1400
BseGI GGATG 4 cut(s) 59, 378, 583, 2058
BseLI CCNNNNNNNGG 6 cut(s) 436, 1093, 1711, 1765, 1864, 2077
BseMI GCAATG 1 cut(s) 1292
BseMII CTCAG 3 cut(s) 27, 131, 992
BseNI ACTGG 3 cut(s) 1297, 1438, 1862
BseRI GAGGAG 1 cut(s) 1382
BseXI GCAGC 5 cut(s) 243, 279, 656, 787, 1937
BsgI GTGCAG 1 cut(s) 794
Bsh1236I CGCG 2 cut(s) 432, 501
BshFI GGCC 6 cut(s) 31, 692, 1010, 1124, 1296, 1444
BsiHKAI GWGCWC 3 cut(s) 526, 1057, 1636
BsiHKCI CYCGRG 1 cut(s) 1344
BsiSI CCGG 2 cut(s) 110, 1951
BslFI GGGAC 2 cut(s) 582, 2093
BslI CCNNNNNNNGG 6 cut(s) 436, 1093, 1711, 1765, 1864, 2077
BsmAI GTCTC 3 cut(s) 869, 1996, 2057
BsmFI GGGAC 2 cut(s) 582, 2093
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 6 cut(s) 31, 692, 1010, 1124, 1296, 1444
Bso31I GGTCTC 1 cut(s) 2057
BsoBI CYCGRG 1 cut(s) 1344
Bsp1286I GDGCHC 3 cut(s) 526, 1057, 1636
BspACI CCGC 6 cut(s) 182, 224, 430, 794, 1712, 1936
BspANI GGCC 6 cut(s) 31, 692, 1010, 1124, 1296, 1444
BspCNI CTCAG 3 cut(s) 26, 130, 993
BspFNI CGCG 2 cut(s) 432, 501
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 114, 1770
BspMAI CTGCAG 1 cut(s) 1480
BspPI GGATC 6 cut(s) 54, 1226, 1391, 1559, 1750, 1879
BspQI GCTCTTC 1 cut(s) 1940
BspTNI GGTCTC 1 cut(s) 2057
BsrDI GCAATG 1 cut(s) 1292
BsrI ACTGG 3 cut(s) 1297, 1438, 1862
BssECI CCNNGG 2 cut(s) 383, 1400
BssT1I CCWWGG 1 cut(s) 383
Bst2UI CCWGG 2 cut(s) 1401, 2072
Bst4CI ACNGT 5 cut(s) 157, 253, 475, 1423, 1974
Bst6I CTCTTC 3 cut(s) 582, 690, 1940
BstC8I GCNNGC 5 cut(s) 284, 622, 798, 899, 1212
BstDEI CTNAG 7 cut(s) 13, 117, 611, 682, 1001, 1466, 1685
BstENI CCTNNNNNAGG 2 cut(s) 1091, 1763
BstF5I GGATG 4 cut(s) 59, 378, 583, 2058
BstFNI CGCG 2 cut(s) 432, 501
BstHHI GCGC 1 cut(s) 604
BstMAI GTCTC 3 cut(s) 869, 1996, 2057
BstMWI GCNNNNNNNGC 3 cut(s) 292, 298, 1922
BstNI CCWGG 2 cut(s) 1401, 2072
BstNSI RCATGY 4 cut(s) 610, 620, 901, 1461
BstSCI CCNGG 2 cut(s) 1399, 2070
BstSFI CTRYAG 2 cut(s) 1198, 1476
BstUI CGCG 2 cut(s) 432, 501
BstV1I GCAGC 5 cut(s) 243, 279, 656, 787, 1937
BstV2I GAAGAC 1 cut(s) 979
BstX2I RGATCY 5 cut(s) 887, 1330, 1396, 1551, 1755
BstYI RGATCY 5 cut(s) 887, 1330, 1396, 1551, 1755
BsuRI GGCC 6 cut(s) 31, 692, 1010, 1124, 1296, 1444
BtsCI GGATG 4 cut(s) 59, 378, 583, 2058
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 3 cut(s) 754, 1177, 1869
Cac8I GCNNGC 5 cut(s) 284, 622, 798, 899, 1212
CciI TCATGA 1 cut(s) 937
CfoI GCGC 1 cut(s) 604
Cfr13I GGNCC 2 cut(s) 844, 910
Csp6I GTAC 5 cut(s) 171, 452, 955, 1372, 1750
CspCI CAANNNNNGTGG 2 cut(s) 1078, 1113
CviQI GTAC 5 cut(s) 171, 452, 955, 1372, 1750
DdeI CTNAG 7 cut(s) 13, 117, 611, 682, 1001, 1466, 1685
EaeI YGGCCR 3 cut(s) 29, 1294, 1442
Eam1104I CTCTTC 3 cut(s) 582, 690, 1940
EarI CTCTTC 3 cut(s) 582, 690, 1940
Eco130I CCWWGG 1 cut(s) 383
Eco147I AGGCCT 1 cut(s) 1010
Eco31I GGTCTC 1 cut(s) 2057
Eco47I GGWCC 2 cut(s) 844, 910
Eco57I CTGAAG 1 cut(s) 864
Eco88I CYCGRG 1 cut(s) 1344
EcoNI CCTNNNNNAGG 2 cut(s) 1091, 1763
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 1 cut(s) 1193
EcoRII CCWGG 2 cut(s) 1399, 2070
EcoT14I CCWWGG 1 cut(s) 383
EcoT22I ATGCAT 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 383
FalI AAGNNNNNCTT 2 cut(s) 1122, 1154
FaqI GGGAC 2 cut(s) 582, 2093
FauNDI CATATG 2 cut(s) 583, 948
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 8 cut(s) 182, 225, 257, 293, 645, 776, 1713, 1926
FokI GGATG 4 cut(s) 66, 365, 590, 2045
Fsp4HI GCNGC 8 cut(s) 182, 225, 257, 293, 645, 776, 1713, 1926
FspBI CTAG 5 cut(s) 384, 399, 465, 527, 1359
GlaI GCGC 1 cut(s) 603
GluI GCNGC 8 cut(s) 182, 225, 257, 293, 645, 776, 1713, 1926
GsuI CTGGAG 1 cut(s) 2093
HaeIII GGCC 6 cut(s) 31, 692, 1010, 1124, 1296, 1444
HapII CCGG 2 cut(s) 110, 1951
HhaI GCGC 1 cut(s) 604
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 5 cut(s) 11, 434, 1102, 1512, 1849
HpaII CCGG 2 cut(s) 110, 1951
HphI GGTGA 6 cut(s) 1294, 1411, 1498, 1782, 1816, 1894
Hpy166II GTNNAC 6 cut(s) 10, 160, 171, 497, 1302, 1824
Hpy188I TCNGA 7 cut(s) 883, 1002, 1107, 1335, 1356, 1678, 2027
Hpy188III TCNNGA 4 cut(s) 365, 674, 938, 1346
Hpy8I GTNNAC 6 cut(s) 10, 160, 171, 497, 1302, 1824
Hpy99I CGWCG 1 cut(s) 1221
HpyAV CCTTC 6 cut(s) 95, 682, 702, 1481, 1870, 2042
HpyCH4III ACNGT 5 cut(s) 157, 253, 475, 1423, 1974
HpyF10VI GCNNNNNNNGC 3 cut(s) 292, 298, 1922
HpyF3I CTNAG 7 cut(s) 13, 117, 611, 682, 1001, 1466, 1685
HspAI GCGC 1 cut(s) 602
LguI GCTCTTC 1 cut(s) 1940
LmnI GCTCC 2 cut(s) 112, 1768
Lsp1109I GCAGC 5 cut(s) 243, 279, 656, 787, 1937
LweI GCATC 2 cut(s) 568, 1912
MaeI CTAG 5 cut(s) 384, 399, 465, 527, 1359
MaeIII GTNAC 9 cut(s) 241, 469, 869, 916, 1172, 1324, 1978, 2063, 2093
MboII GAAGA 9 cut(s) 544, 599, 707, 882, 979, 1718, 1851, 1957, 2099
MfeI CAATTG 1 cut(s) 1929
MflI RGATCY 5 cut(s) 887, 1330, 1396, 1551, 1755
MhlI GDGCHC 3 cut(s) 526, 1057, 1636
MlsI TGGCCA 1 cut(s) 1296
MluI ACGCGT 1 cut(s) 499
MluNI TGGCCA 1 cut(s) 1296
MlyI GAGTC 2 cut(s) 5, 1111
MmeI TCCRAC 5 cut(s) 24, 210, 394, 535, 871
Mox20I TGGCCA 1 cut(s) 1296
Mph1103I ATGCAT 1 cut(s) 583
MscI TGGCCA 1 cut(s) 1296
MslI CAYNNNNRTG 1 cut(s) 251
Msp20I TGGCCA 1 cut(s) 1296
MspI CCGG 2 cut(s) 110, 1951
MspR9I CCNGG 2 cut(s) 1401, 2072
MunI CAATTG 1 cut(s) 1929
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 2 cut(s) 1401, 2072
MvnI CGCG 2 cut(s) 432, 501
MwoI GCNNNNNNNGC 3 cut(s) 292, 298, 1922
NdeI CATATG 2 cut(s) 583, 948
NlaIV GGNNCC 2 cut(s) 114, 1770
NmeAIII GCCGAG 1 cut(s) 1470
NmuCI GTSAC 4 cut(s) 916, 1172, 1324, 2063
NsiI ATGCAT 1 cut(s) 583
NspI RCATGY 4 cut(s) 610, 620, 901, 1461
OliI CACNNNNGTG 1 cut(s) 251
PaeI GCATGC 1 cut(s) 901
PagI TCATGA 1 cut(s) 937
PceI AGGCCT 1 cut(s) 1010
PciI ACATGT 1 cut(s) 1457
PciSI GCTCTTC 1 cut(s) 1940
PctI GAATGC 1 cut(s) 739
PfeI GAWTC 3 cut(s) 434, 1512, 1849
PkrI GCNGC 8 cut(s) 183, 226, 258, 294, 646, 777, 1714, 1927
PleI GAGTC 2 cut(s) 5, 1110
PpsI GAGTC 2 cut(s) 5, 1110
PpuMI RGGWCCY 1 cut(s) 910
PscI ACATGT 1 cut(s) 1457
PsiI TTATAA 2 cut(s) 324, 1452
Psp5II RGGWCCY 1 cut(s) 910
Psp6I CCWGG 2 cut(s) 1399, 2070
PspGI CCWGG 2 cut(s) 1399, 2070
PspN4I GGNNCC 2 cut(s) 114, 1770
PspPI GGNCC 2 cut(s) 844, 910
PspPPI RGGWCCY 1 cut(s) 910
PstI CTGCAG 1 cut(s) 1480
PsuI RGATCY 5 cut(s) 887, 1330, 1396, 1551, 1755
RsaI GTAC 5 cut(s) 172, 453, 956, 1373, 1751
RsaNI GTAC 5 cut(s) 171, 452, 955, 1372, 1750
RseI CAYNNNNRTG 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 1940
SatI GCNGC 8 cut(s) 182, 225, 257, 293, 645, 776, 1713, 1926
Sau96I GGNCC 2 cut(s) 844, 910
SchI GAGTC 2 cut(s) 5, 1111
ScrFI CCNGG 2 cut(s) 1401, 2072
SduI GDGCHC 3 cut(s) 526, 1057, 1636
SfaNI GCATC 2 cut(s) 568, 1912
SfcI CTRYAG 2 cut(s) 1198, 1476
SinI GGWCC 2 cut(s) 844, 910
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 703
SmoI CTYRAG 1 cut(s) 703
SpeI ACTAGT 1 cut(s) 526
SphI GCATGC 1 cut(s) 901
SseBI AGGCCT 1 cut(s) 1010
SsiI CCGC 6 cut(s) 182, 224, 430, 794, 1712, 1936
SspI AATATT 1 cut(s) 807
SspMI CTAG 5 cut(s) 384, 399, 465, 527, 1359
StuI AGGCCT 1 cut(s) 1010
StyD4I CCNGG 2 cut(s) 1399, 2070
StyI CCWWGG 1 cut(s) 383
TaaI ACNGT 5 cut(s) 157, 253, 475, 1423, 1974
TaqI TCGA 6 cut(s) 39, 68, 445, 930, 1590, 1619
TatI WGTACW 2 cut(s) 451, 1749
TauI GCSGC 3 cut(s) 184, 227, 1715
TfiI GAWTC 3 cut(s) 434, 1512, 1849
TscAI CASTG 3 cut(s) 754, 1177, 1869
TseFI GTSAC 4 cut(s) 916, 1172, 1324, 2063
TseI GCWGC 5 cut(s) 256, 292, 644, 775, 1925
Tsp45I GTSAC 4 cut(s) 916, 1172, 1324, 2063
TspDTI ATGAA 8 cut(s) 367, 600, 926, 1269, 1292, 1533, 1718, 1958
TspGWI ACGGA 3 cut(s) 118, 1997, 2051
TspRI CASTG 3 cut(s) 754, 1177, 1869
VpaK11BI GGWCC 2 cut(s) 844, 910
XagI CCTNNNNNAGG 2 cut(s) 1091, 1763
XapI RAATTY 2 cut(s) 1193, 1680
XceI RCATGY 4 cut(s) 610, 620, 901, 1461
XmaJI CCTAGG 1 cut(s) 383
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 5 cut(s) 384, 399, 465, 527, 1359
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.