Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 83 |
| Acc36I | ACCTGC | 1 cut(s) | 846 |
| AccI | GTMKAC | 1 cut(s) | 645 |
| AclWI | GGATC | 5 cut(s) | 135, 185, 203, 275, 288 |
| AcuI | CTGAAG | 1 cut(s) | 402 |
| AfaI | GTAC | 1 cut(s) | 274 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 538 |
| AflIII | ACRYGT | 2 cut(s) | 648, 783 |
| AgsI | TTSAA | 5 cut(s) | 190, 351, 659, 790, 846 |
| AjnI | CCWGG | 1 cut(s) | 91 |
| AluBI | AGCT | 7 cut(s) | 55, 228, 247, 307, 628, 704, 862 |
| AluI | AGCT | 7 cut(s) | 55, 228, 247, 307, 628, 704, 862 |
| Alw26I | GTCTC | 1 cut(s) | 320 |
| AlwI | GGATC | 5 cut(s) | 135, 185, 203, 275, 288 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 862 |
| AoxI | GGCC | 1 cut(s) | 3 |
| ApeKI | GCWGC | 2 cut(s) | 55, 394 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 64, 96 |
| AspLEI | GCGC | 1 cut(s) | 728 |
| AsuHPI | GGTGA | 2 cut(s) | 565, 877 |
| BamHI | GGATCC | 1 cut(s) | 280 |
| BauI | CACGAG | 1 cut(s) | 484 |
| BbvI | GCAGC | 2 cut(s) | 42, 381 |
| BccI | CCATC | 2 cut(s) | 150, 408 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 121, 155 |
| BciT130I | CCWGG | 1 cut(s) | 93 |
| BcoDI | GTCTC | 1 cut(s) | 320 |
| BfaI | CTAG | 2 cut(s) | 629, 761 |
| BfmI | CTRYAG | 3 cut(s) | 50, 167, 395 |
| BfoI | RGCGCY | 1 cut(s) | 729 |
| BfuAI | ACCTGC | 1 cut(s) | 846 |
| BisI | GCNGC | 2 cut(s) | 56, 395 |
| BlsI | GCNGC | 2 cut(s) | 57, 396 |
| Bme1390I | CCNGG | 1 cut(s) | 93 |
| BmiI | GGNNCC | 1 cut(s) | 282 |
| BmrFI | CCNGG | 1 cut(s) | 93 |
| BmsI | GCATC | 3 cut(s) | 198, 381, 670 |
| BpmI | CTGGAG | 1 cut(s) | 759 |
| BpuEI | CTTGAG | 1 cut(s) | 826 |
| BsaJI | CCNNGG | 1 cut(s) | 92 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 538 |
| Bse1I | ACTGG | 1 cut(s) | 727 |
| Bse3DI | GCAATG | 1 cut(s) | 26 |
| BseBI | CCWGG | 1 cut(s) | 93 |
| BseDI | CCNNGG | 1 cut(s) | 92 |
| BseGI | GGATG | 3 cut(s) | 161, 213, 685 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 538 |
| BseMI | GCAATG | 1 cut(s) | 26 |
| BseMII | CTCAG | 3 cut(s) | 233, 243, 290 |
| BseNI | ACTGG | 1 cut(s) | 727 |
| BseRI | GAGGAG | 1 cut(s) | 502 |
| BseXI | GCAGC | 2 cut(s) | 42, 381 |
| Bsh1285I | CGRYCG | 1 cut(s) | 472 |
| BshFI | GGCC | 1 cut(s) | 5 |
| BsiEI | CGRYCG | 1 cut(s) | 472 |
| BslFI | GGGAC | 1 cut(s) | 89 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 538 |
| BsmAI | GTCTC | 1 cut(s) | 320 |
| BsmFI | GGGAC | 1 cut(s) | 89 |
| BsmI | GAATGC | 3 cut(s) | 562, 806, 841 |
| BsnI | GGCC | 1 cut(s) | 5 |
| Bsp143I | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| BspANI | GGCC | 1 cut(s) | 5 |
| BspCNI | CTCAG | 3 cut(s) | 232, 242, 289 |
| BspHI | TCATGA | 1 cut(s) | 547 |
| BspLI | GGNNCC | 1 cut(s) | 282 |
| BspMAI | CTGCAG | 2 cut(s) | 171, 399 |
| BspMI | ACCTGC | 1 cut(s) | 846 |
| BspPI | GGATC | 5 cut(s) | 135, 185, 203, 275, 288 |
| BsrDI | GCAATG | 1 cut(s) | 26 |
| BsrI | ACTGG | 1 cut(s) | 727 |
| BssECI | CCNNGG | 1 cut(s) | 92 |
| BssMI | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| BssSI | CACGAG | 1 cut(s) | 484 |
| Bst2BI | CACGAG | 1 cut(s) | 484 |
| Bst2UI | CCWGG | 1 cut(s) | 93 |
| Bst4CI | ACNGT | 4 cut(s) | 80, 109, 259, 355 |
| Bst6I | CTCTTC | 2 cut(s) | 185, 378 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 838 |
| BstDEI | CTNAG | 3 cut(s) | 219, 229, 276 |
| BstF5I | GGATG | 3 cut(s) | 161, 213, 685 |
| BstH2I | RGCGCY | 1 cut(s) | 729 |
| BstHHI | GCGC | 1 cut(s) | 728 |
| BstKTI | GATC | 6 cut(s) | 130, 180, 198, 283, 472, 586 |
| BstMAI | GTCTC | 1 cut(s) | 320 |
| BstMBI | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| BstMCI | CGRYCG | 1 cut(s) | 472 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 572, 838 |
| BstNI | CCWGG | 1 cut(s) | 93 |
| BstNSI | RCATGY | 3 cut(s) | 652, 712, 787 |
| BstSCI | CCNGG | 1 cut(s) | 91 |
| BstSFI | CTRYAG | 3 cut(s) | 50, 167, 395 |
| BstV1I | GCAGC | 2 cut(s) | 42, 381 |
| BstX2I | RGATCY | 1 cut(s) | 280 |
| BstYI | RGATCY | 1 cut(s) | 280 |
| BsuRI | GGCC | 1 cut(s) | 5 |
| BtsCI | GGATG | 3 cut(s) | 161, 213, 685 |
| BtsI | GCAGTG | 1 cut(s) | 856 |
| BtsIMutI | CAGTG | 2 cut(s) | 720, 856 |
| BveI | ACCTGC | 1 cut(s) | 846 |
| CaiI | CAGNNNCTG | 1 cut(s) | 862 |
| CciI | TCATGA | 1 cut(s) | 547 |
| CfoI | GCGC | 1 cut(s) | 728 |
| CseI | GACGC | 1 cut(s) | 735 |
| Csp6I | GTAC | 1 cut(s) | 273 |
| CviAII | CATG | 5 cut(s) | 83, 548, 649, 709, 784 |
| CviQI | GTAC | 1 cut(s) | 273 |
| DdeI | CTNAG | 3 cut(s) | 219, 229, 276 |
| DpnI | GATC | 6 cut(s) | 129, 179, 197, 282, 471, 585 |
| DpnII | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 83 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 83 |
| Eam1104I | CTCTTC | 2 cut(s) | 185, 378 |
| EarI | CTCTTC | 2 cut(s) | 185, 378 |
| Eco57I | CTGAAG | 1 cut(s) | 402 |
| EcoRII | CCWGG | 1 cut(s) | 91 |
| FaeI | CATG | 5 cut(s) | 86, 551, 652, 712, 787 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 504, 536 |
| FaqI | GGGAC | 1 cut(s) | 89 |
| FatI | CATG | 5 cut(s) | 82, 547, 648, 708, 783 |
| FblI | GTMKAC | 1 cut(s) | 645 |
| Fnu4HI | GCNGC | 2 cut(s) | 56, 395 |
| FokI | GGATG | 3 cut(s) | 168, 220, 692 |
| Fsp4HI | GCNGC | 2 cut(s) | 56, 395 |
| FspBI | CTAG | 2 cut(s) | 629, 761 |
| GlaI | GCGC | 1 cut(s) | 727 |
| GluI | GCNGC | 2 cut(s) | 56, 395 |
| GsuI | CTGGAG | 1 cut(s) | 759 |
| HaeII | RGCGCY | 1 cut(s) | 729 |
| HaeIII | GGCC | 1 cut(s) | 5 |
| HgaI | GACGC | 1 cut(s) | 735 |
| HhaI | GCGC | 1 cut(s) | 728 |
| Hin1II | CATG | 5 cut(s) | 86, 551, 652, 712, 787 |
| Hin6I | GCGC | 1 cut(s) | 726 |
| HinP1I | GCGC | 1 cut(s) | 726 |
| HinfI | GANTC | 2 cut(s) | 43, 386 |
| HphI | GGTGA | 2 cut(s) | 565, 877 |
| Hpy166II | GTNNAC | 5 cut(s) | 262, 273, 340, 646, 868 |
| Hpy188I | TCNGA | 2 cut(s) | 42, 232 |
| Hpy188III | TCNNGA | 7 cut(s) | 278, 323, 467, 484, 548, 761, 776 |
| Hpy8I | GTNNAC | 5 cut(s) | 262, 273, 340, 646, 868 |
| Hpy99I | CGWCG | 2 cut(s) | 127, 751 |
| HpyAV | CCTTC | 7 cut(s) | 29, 166, 197, 372, 538, 599, 784 |
| HpyCH4III | ACNGT | 4 cut(s) | 80, 109, 259, 355 |
| HpyCH4IV | ACGT | 1 cut(s) | 601 |
| HpyCH4V | TGCA | 6 cut(s) | 31, 169, 397, 594, 841, 902 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 572, 838 |
| HpyF3I | CTNAG | 3 cut(s) | 219, 229, 276 |
| HpySE526I | ACGT | 1 cut(s) | 601 |
| Hsp92II | CATG | 5 cut(s) | 86, 551, 652, 712, 787 |
| HspAI | GCGC | 1 cut(s) | 726 |
| Kzo9I | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| Lsp1109I | GCAGC | 2 cut(s) | 42, 381 |
| LweI | GCATC | 3 cut(s) | 198, 381, 670 |
| MaeI | CTAG | 2 cut(s) | 629, 761 |
| MaeII | ACGT | 1 cut(s) | 601 |
| MaeIII | GTNAC | 1 cut(s) | 447 |
| MalI | GATC | 6 cut(s) | 129, 179, 197, 282, 471, 585 |
| MboI | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| MboII | GAAGA | 5 cut(s) | 202, 245, 395, 646, 762 |
| MflI | RGATCY | 1 cut(s) | 280 |
| MluCI | AATT | 2 cut(s) | 133, 266 |
| MlyI | GAGTC | 2 cut(s) | 37, 395 |
| MnlI | CCTC | 8 cut(s) | 16, 186, 328, 480, 483, 511, 544, 810 |
| MseI | TTAA | 3 cut(s) | 288, 741, 876 |
| MspA1I | CMGCKG | 1 cut(s) | 55 |
| MspR9I | CCNGG | 1 cut(s) | 93 |
| Mva1269I | GAATGC | 3 cut(s) | 562, 806, 841 |
| MvaI | CCWGG | 1 cut(s) | 93 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 572, 838 |
| NdeII | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| NlaIII | CATG | 5 cut(s) | 86, 551, 652, 712, 787 |
| NlaIV | GGNNCC | 1 cut(s) | 282 |
| NmuCI | GTSAC | 1 cut(s) | 447 |
| NspI | RCATGY | 3 cut(s) | 652, 712, 787 |
| PagI | TCATGA | 1 cut(s) | 547 |
| PciI | ACATGT | 2 cut(s) | 648, 783 |
| PctI | GAATGC | 3 cut(s) | 562, 806, 841 |
| PkrI | GCNGC | 2 cut(s) | 57, 396 |
| Ple19I | CGATCG | 1 cut(s) | 472 |
| PleI | GAGTC | 2 cut(s) | 37, 394 |
| PpsI | GAGTC | 2 cut(s) | 37, 394 |
| PscI | ACATGT | 2 cut(s) | 648, 783 |
| Psp6I | CCWGG | 1 cut(s) | 91 |
| PspGI | CCWGG | 1 cut(s) | 91 |
| PspN4I | GGNNCC | 1 cut(s) | 282 |
| PstI | CTGCAG | 2 cut(s) | 171, 399 |
| PstNI | CAGNNNCTG | 1 cut(s) | 862 |
| PsuI | RGATCY | 1 cut(s) | 280 |
| PvuI | CGATCG | 1 cut(s) | 472 |
| PvuII | CAGCTG | 1 cut(s) | 55 |
| RsaI | GTAC | 1 cut(s) | 274 |
| RsaNI | GTAC | 1 cut(s) | 273 |
| SaqAI | TTAA | 3 cut(s) | 288, 741, 876 |
| SatI | GCNGC | 2 cut(s) | 56, 395 |
| Sau3AI | GATC | 6 cut(s) | 127, 177, 195, 280, 469, 583 |
| SchI | GAGTC | 2 cut(s) | 37, 395 |
| ScrFI | CCNGG | 1 cut(s) | 93 |
| SfaNI | GCATC | 3 cut(s) | 198, 381, 670 |
| SfcI | CTRYAG | 3 cut(s) | 50, 167, 395 |
| SmlI | CTYRAG | 1 cut(s) | 805 |
| SmoI | CTYRAG | 1 cut(s) | 805 |
| Sse9I | AATT | 2 cut(s) | 133, 266 |
| SspMI | CTAG | 2 cut(s) | 629, 761 |
| StyD4I | CCNGG | 1 cut(s) | 91 |
| TaaI | ACNGT | 4 cut(s) | 80, 109, 259, 355 |
| TaiI | ACGT | 1 cut(s) | 604 |
| TaqI | TCGA | 6 cut(s) | 122, 141, 389, 468, 749, 795 |
| TasI | AATT | 2 cut(s) | 133, 266 |
| TatI | WGTACW | 1 cut(s) | 272 |
| Tru1I | TTAA | 3 cut(s) | 288, 741, 876 |
| Tru9I | TTAA | 3 cut(s) | 288, 741, 876 |
| TscAI | CASTG | 2 cut(s) | 727, 856 |
| TseFI | GTSAC | 1 cut(s) | 447 |
| TseI | GCWGC | 2 cut(s) | 55, 394 |
| Tsp45I | GTSAC | 1 cut(s) | 447 |
| TspDTI | ATGAA | 6 cut(s) | 127, 257, 449, 469, 525, 813 |
| TspGWI | ACGGA | 3 cut(s) | 140, 168, 521 |
| TspRI | CASTG | 2 cut(s) | 727, 856 |
| XbaI | TCTAGA | 1 cut(s) | 760 |
| XceI | RCATGY | 3 cut(s) | 652, 712, 787 |
| XmiI | GTMKAC | 1 cut(s) | 645 |
| XspI | CTAG | 2 cut(s) | 629, 761 |