Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1551 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1514 |
| Acc36I | ACCTGC | 1 cut(s) | 410 |
| AccII | CGCG | 1 cut(s) | 2163 |
| AccIII | TCCGGA | 1 cut(s) | 652 |
| AciI | CCGC | 3 cut(s) | 274, 1154, 1166 |
| AclWI | GGATC | 5 cut(s) | 33, 62, 67, 80, 887 |
| AcoI | YGGCCR | 1 cut(s) | 1541 |
| AcsI | RAATTY | 3 cut(s) | 1292, 1873, 1907 |
| AcuI | CTGAAG | 4 cut(s) | 86, 951, 978, 1596 |
| AcvI | CACGTG | 1 cut(s) | 1668 |
| AfaI | GTAC | 4 cut(s) | 151, 659, 937, 1850 |
| AfeI | AGCGCT | 1 cut(s) | 1756 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1140, 1192, 2176 |
| AflIII | ACRYGT | 3 cut(s) | 144, 660, 2196 |
| AjnI | CCWGG | 3 cut(s) | 975, 1498, 2169 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 83, 115 |
| Alw21I | GWGCWC | 1 cut(s) | 505 |
| AlwI | GGATC | 5 cut(s) | 33, 62, 67, 80, 887 |
| Ama87I | CYCGRG | 2 cut(s) | 981, 1443 |
| Aor13HI | TCCGGA | 1 cut(s) | 652 |
| Aor51HI | AGCGCT | 1 cut(s) | 1756 |
| AoxI | GGCC | 3 cut(s) | 939, 1221, 1541 |
| ApeKI | GCWGC | 9 cut(s) | 271, 623, 965, 1033, 1255, 1381, 1730, 2024, 2095 |
| ApoI | RAATTY | 3 cut(s) | 1292, 1873, 1907 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2108 |
| AspLEI | GCGC | 2 cut(s) | 9, 1757 |
| AspS9I | GGNCC | 3 cut(s) | 1009, 1348, 1447 |
| AsuC2I | CCSGG | 1 cut(s) | 200 |
| AsuHPI | GGTGA | 3 cut(s) | 1469, 1510, 1597 |
| AvaI | CYCGRG | 2 cut(s) | 981, 1443 |
| AvaII | GGWCC | 3 cut(s) | 1009, 1348, 1447 |
| BaeGI | GKGCMC | 1 cut(s) | 1667 |
| BalI | TGGCCA | 1 cut(s) | 1543 |
| BamHI | GGATCC | 1 cut(s) | 72 |
| BbrPI | CACGTG | 1 cut(s) | 1668 |
| BbsI | GAAGAC | 1 cut(s) | 2154 |
| Bbv12I | GWGCWC | 1 cut(s) | 505 |
| BbvI | GCAGC | 9 cut(s) | 258, 635, 952, 1020, 1242, 1393, 1717, 2011, 2107 |
| BccI | CCATC | 6 cut(s) | 27, 143, 285, 1498, 1576, 1892 |
| BceAI | ACGGC | 2 cut(s) | 954, 1475 |
| BciT130I | CCWGG | 3 cut(s) | 977, 1500, 2171 |
| BcnI | CCSGG | 1 cut(s) | 200 |
| BfaI | CTAG | 4 cut(s) | 77, 590, 852, 1310 |
| BfmI | CTRYAG | 1 cut(s) | 1297 |
| BfoI | RGCGCY | 1 cut(s) | 1758 |
| BfuAI | ACCTGC | 1 cut(s) | 410 |
| BisI | GCNGC | 9 cut(s) | 272, 624, 966, 1034, 1256, 1382, 1731, 2025, 2096 |
| BlsI | GCNGC | 9 cut(s) | 273, 625, 967, 1035, 1257, 1383, 1732, 2026, 2097 |
| Bme1390I | CCNGG | 4 cut(s) | 200, 977, 1500, 2171 |
| Bme18I | GGWCC | 3 cut(s) | 1009, 1348, 1447 |
| BmeT110I | CYCGRG | 2 cut(s) | 981, 1443 |
| BmgT120I | GGNCC | 3 cut(s) | 1009, 1348, 1447 |
| BmiI | GGNNCC | 4 cut(s) | 74, 93, 1448, 1869 |
| BmrFI | CCNGG | 4 cut(s) | 200, 977, 1500, 2171 |
| BmsI | GCATC | 3 cut(s) | 225, 952, 2011 |
| BpiI | GAAGAC | 1 cut(s) | 2154 |
| BpmI | CTGGAG | 1 cut(s) | 2192 |
| BpuEI | CTTGAG | 4 cut(s) | 812, 1172, 1259, 1361 |
| BpuMI | CCSGG | 1 cut(s) | 200 |
| Bsa29I | ATCGAT | 1 cut(s) | 2019 |
| BsaAI | YACGTR | 1 cut(s) | 1668 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1859 |
| BsaJI | CCNNGG | 2 cut(s) | 942, 1499 |
| BsaWI | WCCGGW | 3 cut(s) | 652, 1635, 2004 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 83, 113, 647, 677, 1135, 1165 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1140, 1192, 2176 |
| Bse1I | ACTGG | 3 cut(s) | 1537, 1544, 1684 |
| Bse3DI | GCAATG | 3 cut(s) | 357, 1391, 1678 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1859 |
| BseAI | TCCGGA | 1 cut(s) | 652 |
| BseBI | CCWGG | 3 cut(s) | 977, 1500, 2171 |
| BseCI | ATCGAT | 1 cut(s) | 2019 |
| BseDI | CCNNGG | 2 cut(s) | 942, 1499 |
| BseGI | GGATG | 4 cut(s) | 38, 815, 847, 1509 |
| BseJI | GATNNNNATC | 1 cut(s) | 1859 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1140, 1192, 2176 |
| BseMI | GCAATG | 3 cut(s) | 357, 1391, 1678 |
| BseMII | CTCAG | 3 cut(s) | 110, 815, 1802 |
| BseNI | ACTGG | 3 cut(s) | 1537, 1544, 1684 |
| BseRI | GAGGAG | 2 cut(s) | 1481, 1733 |
| BseSI | GKGCMC | 1 cut(s) | 1667 |
| BseXI | GCAGC | 9 cut(s) | 258, 635, 952, 1020, 1242, 1393, 1717, 2011, 2107 |
| BseYI | CCCAGC | 1 cut(s) | 2098 |
| BsgI | GTGCAG | 1 cut(s) | 363 |
| Bsh1236I | CGCG | 1 cut(s) | 2163 |
| BshFI | GGCC | 3 cut(s) | 941, 1223, 1543 |
| BshVI | ATCGAT | 1 cut(s) | 2019 |
| BsiHKAI | GWGCWC | 1 cut(s) | 505 |
| BsiHKCI | CYCGRG | 2 cut(s) | 981, 1443 |
| BsiSI | CCGG | 4 cut(s) | 199, 653, 1636, 2005 |
| BslFI | GGGAC | 3 cut(s) | 50, 1460, 2192 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1140, 1192, 2176 |
| BsmFI | GGGAC | 3 cut(s) | 50, 1460, 2192 |
| BsmI | GAATGC | 2 cut(s) | 445, 683 |
| BsnI | GGCC | 3 cut(s) | 941, 1223, 1543 |
| BsoBI | CYCGRG | 2 cut(s) | 981, 1443 |
| Bsp1286I | GDGCHC | 2 cut(s) | 505, 1667 |
| Bsp13I | TCCGGA | 1 cut(s) | 652 |
| Bsp68I | TCGCGA | 1 cut(s) | 2163 |
| BspACI | CCGC | 3 cut(s) | 274, 1154, 1166 |
| BspANI | GGCC | 3 cut(s) | 941, 1223, 1543 |
| BspCNI | CTCAG | 3 cut(s) | 109, 816, 1801 |
| BspDI | ATCGAT | 1 cut(s) | 2019 |
| BspEI | TCCGGA | 1 cut(s) | 652 |
| BspFNI | CGCG | 1 cut(s) | 2163 |
| BspHI | TCATGA | 1 cut(s) | 1804 |
| BspLI | GGNNCC | 4 cut(s) | 74, 93, 1448, 1869 |
| BspMI | ACCTGC | 1 cut(s) | 410 |
| BspPI | GGATC | 5 cut(s) | 33, 62, 67, 80, 887 |
| BsrDI | GCAATG | 3 cut(s) | 357, 1391, 1678 |
| BsrI | ACTGG | 3 cut(s) | 1537, 1544, 1684 |
| BssECI | CCNNGG | 2 cut(s) | 942, 1499 |
| BssT1I | CCWWGG | 1 cut(s) | 942 |
| Bst2UI | CCWGG | 3 cut(s) | 977, 1500, 2171 |
| Bst4CI | ACNGT | 5 cut(s) | 232, 507, 1398, 1516, 1522 |
| Bst6I | CTCTTC | 4 cut(s) | 62, 1572, 1751, 2039 |
| BstBAI | YACGTR | 1 cut(s) | 1668 |
| BstC8I | GCNNGC | 3 cut(s) | 240, 258, 1260 |
| BstDEI | CTNAG | 4 cut(s) | 96, 245, 824, 1788 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1190 |
| BstF5I | GGATG | 4 cut(s) | 38, 815, 847, 1509 |
| BstFNI | CGCG | 1 cut(s) | 2163 |
| BstH2I | RGCGCY | 1 cut(s) | 1758 |
| BstHHI | GCGC | 2 cut(s) | 9, 1757 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 277, 449, 962, 1381, 1831, 2021 |
| BstNI | CCWGG | 3 cut(s) | 977, 1500, 2171 |
| BstNSI | RCATGY | 4 cut(s) | 148, 260, 664, 2200 |
| BstSCI | CCNGG | 4 cut(s) | 198, 975, 1498, 2169 |
| BstSFI | CTRYAG | 1 cut(s) | 1297 |
| BstSLI | GKGCMC | 1 cut(s) | 1667 |
| BstUI | CGCG | 1 cut(s) | 2163 |
| BstV1I | GCAGC | 9 cut(s) | 258, 635, 952, 1020, 1242, 1393, 1717, 2011, 2107 |
| BstV2I | GAAGAC | 1 cut(s) | 2154 |
| BstX2I | RGATCY | 1 cut(s) | 72 |
| BstYI | RGATCY | 1 cut(s) | 72 |
| Bsu15I | ATCGAT | 1 cut(s) | 2019 |
| BsuRI | GGCC | 3 cut(s) | 941, 1223, 1543 |
| BsuTUI | ATCGAT | 1 cut(s) | 2019 |
| BtgZI | GCGATG | 1 cut(s) | 1230 |
| BtsCI | GGATG | 4 cut(s) | 38, 815, 847, 1509 |
| BtsI | GCAGTG | 1 cut(s) | 733 |
| BtsIMutI | CAGTG | 5 cut(s) | 237, 503, 733, 1276, 1425 |
| BtuMI | TCGCGA | 1 cut(s) | 2163 |
| BveI | ACCTGC | 1 cut(s) | 410 |
| Cac8I | GCNNGC | 3 cut(s) | 240, 258, 1260 |
| CciI | TCATGA | 1 cut(s) | 1804 |
| CfoI | GCGC | 2 cut(s) | 9, 1757 |
| Cfr13I | GGNCC | 3 cut(s) | 1009, 1348, 1447 |
| ClaI | ATCGAT | 1 cut(s) | 2019 |
| Csp6I | GTAC | 4 cut(s) | 150, 658, 936, 1849 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1177, 1212 |
| CviAII | CATG | 9 cut(s) | 145, 257, 661, 758, 1037, 1647, 1805, 1839, 2197 |
| CviQI | GTAC | 4 cut(s) | 150, 658, 936, 1849 |
| DdeI | CTNAG | 4 cut(s) | 96, 245, 824, 1788 |
| DraI | TTTAAA | 1 cut(s) | 913 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1514 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1514 |
| EaeI | YGGCCR | 1 cut(s) | 1541 |
| Eam1104I | CTCTTC | 4 cut(s) | 62, 1572, 1751, 2039 |
| EarI | CTCTTC | 4 cut(s) | 62, 1572, 1751, 2039 |
| Eco130I | CCWWGG | 1 cut(s) | 942 |
| Eco47I | GGWCC | 3 cut(s) | 1009, 1348, 1447 |
| Eco47III | AGCGCT | 1 cut(s) | 1756 |
| Eco57I | CTGAAG | 4 cut(s) | 86, 951, 978, 1596 |
| Eco72I | CACGTG | 1 cut(s) | 1668 |
| Eco88I | CYCGRG | 2 cut(s) | 981, 1443 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1190 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1009 |
| EcoRI | GAATTC | 2 cut(s) | 1292, 1907 |
| EcoRII | CCWGG | 3 cut(s) | 975, 1498, 2169 |
| EcoT14I | CCWWGG | 1 cut(s) | 942 |
| ErhI | CCWWGG | 1 cut(s) | 942 |
| FaeI | CATG | 9 cut(s) | 148, 260, 664, 761, 1040, 1650, 1808, 1842, 2200 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1221, 1253 |
| FaqI | GGGAC | 3 cut(s) | 50, 1460, 2192 |
| FatI | CATG | 9 cut(s) | 144, 256, 660, 757, 1036, 1646, 1804, 1838, 2196 |
| FauI | CCCGC | 2 cut(s) | 267, 1159 |
| Fnu4HI | GCNGC | 9 cut(s) | 272, 624, 966, 1034, 1256, 1382, 1731, 2025, 2096 |
| FokI | GGATG | 4 cut(s) | 45, 822, 854, 1516 |
| Fsp4HI | GCNGC | 9 cut(s) | 272, 624, 966, 1034, 1256, 1382, 1731, 2025, 2096 |
| FspBI | CTAG | 4 cut(s) | 77, 590, 852, 1310 |
| GlaI | GCGC | 2 cut(s) | 8, 1756 |
| GluI | GCNGC | 9 cut(s) | 272, 624, 966, 1034, 1256, 1382, 1731, 2025, 2096 |
| GsaI | CCCAGC | 1 cut(s) | 2102 |
| GsuI | CTGGAG | 1 cut(s) | 2192 |
| HaeII | RGCGCY | 1 cut(s) | 1758 |
| HaeIII | GGCC | 3 cut(s) | 941, 1223, 1543 |
| HapII | CCGG | 4 cut(s) | 199, 653, 1636, 2005 |
| HhaI | GCGC | 2 cut(s) | 9, 1757 |
| Hin1II | CATG | 9 cut(s) | 148, 260, 664, 761, 1040, 1650, 1808, 1842, 2200 |
| Hin6I | GCGC | 2 cut(s) | 7, 1755 |
| HinP1I | GCGC | 2 cut(s) | 7, 1755 |
| HincII | GTYRAC | 3 cut(s) | 706, 1719, 1996 |
| HindII | GTYRAC | 3 cut(s) | 706, 1719, 1996 |
| HindIII | AAGCTT | 1 cut(s) | 737 |
| HinfI | GANTC | 8 cut(s) | 1170, 1495, 1611, 1786, 1855, 1941, 1948, 2047 |
| HpaI | GTTAAC | 1 cut(s) | 706 |
| HpaII | CCGG | 4 cut(s) | 199, 653, 1636, 2005 |
| HphI | GGTGA | 3 cut(s) | 1469, 1510, 1597 |
| Hpy166II | GTNNAC | 8 cut(s) | 150, 706, 731, 1394, 1401, 1719, 1923, 1996 |
| Hpy188I | TCNGA | 5 cut(s) | 1074, 1335, 1368, 1777, 2052 |
| Hpy8I | GTNNAC | 8 cut(s) | 150, 706, 731, 1394, 1401, 1719, 1923, 1996 |
| Hpy99I | CGWCG | 1 cut(s) | 1320 |
| HpyAV | CCTTC | 9 cut(s) | 43, 360, 661, 725, 790, 926, 1186, 1237, 1580 |
| HpyCH4III | ACNGT | 5 cut(s) | 232, 507, 1398, 1516, 1522 |
| HpyCH4IV | ACGT | 3 cut(s) | 478, 1403, 1667 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 277, 449, 962, 1381, 1831, 2021 |
| HpyF3I | CTNAG | 4 cut(s) | 96, 245, 824, 1788 |
| HpySE526I | ACGT | 3 cut(s) | 478, 1403, 1667 |
| Hsp92II | CATG | 9 cut(s) | 148, 260, 664, 761, 1040, 1650, 1808, 1842, 2200 |
| HspAI | GCGC | 2 cut(s) | 7, 1755 |
| Kpn2I | TCCGGA | 1 cut(s) | 652 |
| KspAI | GTTAAC | 1 cut(s) | 706 |
| LmnI | GCTCC | 5 cut(s) | 4, 91, 277, 1746, 1867 |
| Lsp1109I | GCAGC | 9 cut(s) | 258, 635, 952, 1020, 1242, 1393, 1717, 2011, 2107 |
| LweI | GCATC | 3 cut(s) | 225, 952, 2011 |
| MaeI | CTAG | 4 cut(s) | 77, 590, 852, 1310 |
| MaeII | ACGT | 3 cut(s) | 478, 1403, 1667 |
| MaeIII | GTNAC | 8 cut(s) | 84, 369, 1271, 1320, 1423, 1516, 1535, 1675 |
| MfeI | CAATTG | 1 cut(s) | 2028 |
| MflI | RGATCY | 1 cut(s) | 72 |
| MhlI | GDGCHC | 2 cut(s) | 505, 1667 |
| MlsI | TGGCCA | 1 cut(s) | 1543 |
| MluNI | TGGCCA | 1 cut(s) | 1543 |
| MlyI | GAGTC | 2 cut(s) | 1780, 2056 |
| MmeI | TCCRAC | 2 cut(s) | 373, 2030 |
| Mox20I | TGGCCA | 1 cut(s) | 1543 |
| MroI | TCCGGA | 1 cut(s) | 652 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2108 |
| MscI | TGGCCA | 1 cut(s) | 1543 |
| MseI | TTAA | 5 cut(s) | 705, 912, 1251, 1566, 2133 |
| Msp20I | TGGCCA | 1 cut(s) | 1543 |
| MspA1I | CMGCKG | 1 cut(s) | 2098 |
| MspI | CCGG | 4 cut(s) | 199, 653, 1636, 2005 |
| MspR9I | CCNGG | 4 cut(s) | 200, 977, 1500, 2171 |
| MunI | CAATTG | 1 cut(s) | 2028 |
| Mva1269I | GAATGC | 2 cut(s) | 445, 683 |
| MvaI | CCWGG | 3 cut(s) | 977, 1500, 2171 |
| MvnI | CGCG | 1 cut(s) | 2163 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 277, 449, 962, 1381, 1831, 2021 |
| NciI | CCSGG | 1 cut(s) | 200 |
| NlaIII | CATG | 9 cut(s) | 148, 260, 664, 761, 1040, 1650, 1808, 1842, 2200 |
| NlaIV | GGNNCC | 4 cut(s) | 74, 93, 1448, 1869 |
| NmeAIII | GCCGAG | 1 cut(s) | 286 |
| NmuCI | GTSAC | 4 cut(s) | 369, 1271, 1423, 1516 |
| NruI | TCGCGA | 1 cut(s) | 2163 |
| NspI | RCATGY | 4 cut(s) | 148, 260, 664, 2200 |
| PaeI | GCATGC | 1 cut(s) | 260 |
| PaeR7I | CTCGAG | 1 cut(s) | 981 |
| PagI | TCATGA | 1 cut(s) | 1804 |
| PciI | ACATGT | 3 cut(s) | 144, 660, 2196 |
| PctI | GAATGC | 2 cut(s) | 445, 683 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2108 |
| PfeI | GAWTC | 6 cut(s) | 1170, 1495, 1611, 1855, 1941, 1948 |
| PkrI | GCNGC | 9 cut(s) | 273, 625, 967, 1035, 1257, 1383, 1732, 2026, 2097 |
| PleI | GAGTC | 2 cut(s) | 1780, 2055 |
| PmaCI | CACGTG | 1 cut(s) | 1668 |
| PmlI | CACGTG | 1 cut(s) | 1668 |
| PpsI | GAGTC | 2 cut(s) | 1780, 2055 |
| Ppu21I | YACGTR | 1 cut(s) | 1668 |
| PpuMI | RGGWCCY | 1 cut(s) | 1009 |
| PscI | ACATGT | 3 cut(s) | 144, 660, 2196 |
| PsiI | TTATAA | 1 cut(s) | 1551 |
| Psp5II | RGGWCCY | 1 cut(s) | 1009 |
| Psp6I | CCWGG | 3 cut(s) | 975, 1498, 2169 |
| PspCI | CACGTG | 1 cut(s) | 1668 |
| PspFI | CCCAGC | 1 cut(s) | 2098 |
| PspGI | CCWGG | 3 cut(s) | 975, 1498, 2169 |
| PspN4I | GGNNCC | 4 cut(s) | 74, 93, 1448, 1869 |
| PspPI | GGNCC | 3 cut(s) | 1009, 1348, 1447 |
| PspPPI | RGGWCCY | 1 cut(s) | 1009 |
| PspXI | VCTCGAGB | 1 cut(s) | 981 |
| PsuI | RGATCY | 1 cut(s) | 72 |
| PvuII | CAGCTG | 1 cut(s) | 2098 |
| RruI | TCGCGA | 1 cut(s) | 2163 |
| RsaI | GTAC | 4 cut(s) | 151, 659, 937, 1850 |
| RsaNI | GTAC | 4 cut(s) | 150, 658, 936, 1849 |
| SaqAI | TTAA | 5 cut(s) | 705, 912, 1251, 1566, 2133 |
| SatI | GCNGC | 9 cut(s) | 272, 624, 966, 1034, 1256, 1382, 1731, 2025, 2096 |
| Sau96I | GGNCC | 3 cut(s) | 1009, 1348, 1447 |
| SchI | GAGTC | 2 cut(s) | 1780, 2056 |
| ScrFI | CCNGG | 4 cut(s) | 200, 977, 1500, 2171 |
| SduI | GDGCHC | 2 cut(s) | 505, 1667 |
| SfaNI | GCATC | 3 cut(s) | 225, 952, 2011 |
| SfcI | CTRYAG | 1 cut(s) | 1297 |
| Sfr274I | CTCGAG | 1 cut(s) | 981 |
| SinI | GGWCC | 3 cut(s) | 1009, 1348, 1447 |
| SlaI | CTCGAG | 1 cut(s) | 981 |
| SmlI | CTYRAG | 5 cut(s) | 791, 981, 1187, 1238, 1376 |
| SmoI | CTYRAG | 5 cut(s) | 791, 981, 1187, 1238, 1376 |
| SphI | GCATGC | 1 cut(s) | 260 |
| SsiI | CCGC | 3 cut(s) | 274, 1154, 1166 |
| SspI | AATATT | 1 cut(s) | 1782 |
| SspMI | CTAG | 4 cut(s) | 77, 590, 852, 1310 |
| StyD4I | CCNGG | 4 cut(s) | 198, 975, 1498, 2169 |
| StyI | CCWWGG | 1 cut(s) | 942 |
| TaaI | ACNGT | 5 cut(s) | 232, 507, 1398, 1516, 1522 |
| TaiI | ACGT | 3 cut(s) | 481, 1406, 1670 |
| TaqI | TCGA | 4 cut(s) | 665, 891, 982, 2019 |
| TatI | WGTACW | 3 cut(s) | 149, 657, 1848 |
| TfiI | GAWTC | 6 cut(s) | 1170, 1495, 1611, 1855, 1941, 1948 |
| Tru1I | TTAA | 5 cut(s) | 705, 912, 1251, 1566, 2133 |
| Tru9I | TTAA | 5 cut(s) | 705, 912, 1251, 1566, 2133 |
| TscAI | CASTG | 5 cut(s) | 237, 510, 733, 1276, 1432 |
| TseFI | GTSAC | 4 cut(s) | 369, 1271, 1423, 1516 |
| TseI | GCWGC | 9 cut(s) | 271, 623, 965, 1033, 1255, 1381, 1730, 2024, 2095 |
| Tsp45I | GTSAC | 4 cut(s) | 369, 1271, 1423, 1516 |
| TspDTI | ATGAA | 9 cut(s) | 346, 402, 579, 707, 1064, 1632, 1817, 1983, 2057 |
| TspGWI | ACGGA | 2 cut(s) | 1123, 1940 |
| TspRI | CASTG | 5 cut(s) | 237, 510, 733, 1276, 1432 |
| VpaK11BI | GGWCC | 3 cut(s) | 1009, 1348, 1447 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1190 |
| XapI | RAATTY | 3 cut(s) | 1292, 1873, 1907 |
| XceI | RCATGY | 4 cut(s) | 148, 260, 664, 2200 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 983 |
| XhoI | CTCGAG | 1 cut(s) | 981 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2108 |
| XspI | CTAG | 4 cut(s) | 77, 590, 852, 1310 |