Rw3G016800

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000162
Physical Location & Seq
Reverse (-)
17987856 .. 17990628
2773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016800.1

Sequence Viewer

Length: 1398 bp
ATGCAGAAGGAAAAGGAGAGGGCTTCTCATTACTTGTATTCGACCAGTGAGCCGAAGCTGCTGGTGAAAGTGCAGCATGAGTTGTTGGTGGTTTATTACACCCAAATCCTTGATAAGGAGGAATCTGGATGTTGGGTTTTGCTTCAGAATGATAAGGTGGAGGATTTGTCTAATATATATAGGCTCTACCTTAATATACCTATAGGCTTGGAACCTGTTGCTAGCGTATTCAGAGAGCATGTTACTAATGAAGGTAATGCCTTGGTCCAACAGGCTGAAGAGGCTGTTGCAAGTAGTAATCAGCAGGCTTCAAGTGGAGCTGAACAATATGGACTTGTGCTTATCCAAAAACTAATAGAGCTGCATGACAAGTATTTGGGATATGTGATTGGTTGCTTTATGAACCATTCACTCTTTCACAAGGCTCTGAAGCAAGCTTTTGAGGTGGTTTACGATAAATCTATTGTCGGGAGTTCTAGTGCCAAAATGCTTGCTGCTTTCTGTGATAATATCCTCAAAATGGCTGGAAGTAAGTTGAGCGAGGAGGCCGTAGAAGAAACCCTAGAAAAGGTTGTTACTCTACTTGTTTATATCAGTGACAAAGACCTCTTTCTGAGTTCTATAGGAAAAAACTTACCCTCTTCTGACAAAGCTGAAGCGCAATGTGGTGGACAATTCACCTCCAAGATGGAAGGAATGATCACCGATTTGACAGTGGAAAAGAATCGAAATCCCAGGCTCAATTTCAGTCACAGTTCTCACAACCGGTTACTGGCATGGCAAAGTTACAAATCGTCTGATCTCAACCTTCCTGAAGAGATGGTCAAGTGTGTTGAAGTTTTCAAGGATTTCTTTAATAGGGAATACCATAGCAGAAAGCTGTCATGGATATACTCACTGGAACTGGTTGTGACAACTCATCAGGCTGCTCTCCTATTGCTCTTTAACAATGCTGAGAAGTTGAGCTTTTCAGAAATCTCAACTCAGATAAATCTAACCATAGACGAGTTGATTAGACTTGCTTCATTCTCTTTCATGTGTGAAATCCTTCTCAAGGAGCCAAGTACAAAGACTATCTTGCAGAGTGACACCTTTATGCTCAACTCCATGTTCACTGACAAAATGAGAAAGGAATTTCCTATGCCACCACTGGACGAAAGGAAGAAGGTGACTGAAGATGTTGACAAAGACTGGAAATTTGCTATAGATGCTGCAATTGTGAGGATTATGAAGAGTAGGAAAGTTTTGGGTCACCAGCAATTGGTCATGGAGTGTGTTGAGATATTGAAGGGCTTGTTCAAGCCGGACATCAAAACAATTAAGAAGCGAATTGAAGATCTCATCACCCATGAATACCTAGAGATGGATATGGAGAATCCTAATAGGTATCTTGCATGA

Protein Analysis

465

Amino Acids

53.3

Weight (kDa)

5.75

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 2 - 205 5e-41 Cullin alpha solenoid domain
Cullin_AB PF26557 259 - 371 4e-16 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 399 - 458 1.6e-23 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000162)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02980 AT1G02980 AT1G43140 AT1G59790 AT1G59790 AT1G59800 AT4G02570 AT4G02570 AT4G02570 AT4G02570
fragaria_vesca FvH4_2g25090 FvH4_2g25090 FvH4_3g22682 FvH4_3g22690 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19660 FvH4_5g19681 FvH4_5g19681 FvH4_5g19691 FvH4_5g19691 FvH4_5g19691 FvH4_5g19700 FvH4_5g19701 FvH4_5g19701 FvH4_5g19702
malus_domestica MD03G1282100.v1.1 MD04G1059100.v1.1 MD04G1059200.v1.1 MD04G1059400.v1.1 MD04G1059700.v1.1 MD06G1052400.v1.1 MD06G1052500.v1.1 MD06G1052800.v1.1 MD11G1301900.v1.1 MD13G1135800.v1.1 MD13G1135900.v1.1 MD13G1170300.v1.1 MD16G1171700.v1.1
prunus_persica Prupe.1G138700_v2.0.a1 Prupe.1G138700_v2.0.a1 Prupe.5G063100_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063200_v2.0.a1 Prupe.5G063300_v2.0.a1 Prupe.5G063500_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.5G063700_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1 Prupe.8G255500_v2.0.a1
pyrus_communis pycom03g22290 pycom04g05320 pycom06g04400 pycom06g04420 pycom06g04430 pycom11g26450 pycom13g11840 pycom13g14580 pycom16g14410 pycom16g14420 pycom16g14430
rosa_chinensis RchiOBHm_Chr3g0471921 RchiOBHm_Chr4g0421701 RchiOBHm_Chr5g0039501 RchiOBHm_Chr5g0039521 RchiOBHm_Chr5g0039591 RchiOBHm_Chr6g0293141 RchiOBHm_Chr7g0204391 RchiOBHm_Chr7g0204411 RchiOBHm_Chr7g0204451 RchiOBHm_Chr7g0204461 RchiOBHm_Chr7g0204501 RchiOBHm_Chr7g0204511
rosa_laevigata RLG00000003454 RLG00000003455 RLG00000003456 RLG00000003458 RLG00000003464 RLG00000003466 RLG00000007657 RLG00000011971 RLG00000024117 RLG00000024118 RLG00000033923 RLG00000033924 RLG00000033929
rosa_multiflora Rmu_co8215636.1_g000001 Rmu_co8492929.1_g000001 Rmu_co8518197.1_g000002 Rmu_sc0000090.1_g000010 Rmu_sc0000090.1_g000014 Rmu_sc0000423.1_g000004 Rmu_sc0000423.1_g000006 Rmu_sc0001042.1_g000001 Rmu_sc0002140.1_g000001 Rmu_sc0002918.1_g000004 Rmu_sc0004533.1_g000009 Rmu_sc0029623.1_g000001 Rmu_ssc0000400.1_g000014 Rmu_ssc0000400.1_g000030 Rmu_ssc0000400.1_g000034 Rmu_ssc0000443.1_g000014 Rmu_ssc0000443.1_g000015 Rmu_ssc0000443.1_g000016
rosa_roxburghii Rroxscaffold_1G00040720 Rroxscaffold_1G00040730 Rroxscaffold_1G00041600 Rroxscaffold_1G00041640 Rroxscaffold_1G00041650 Rroxscaffold_3G00253030 Rroxscaffold_3G00253040 Rroxscaffold_3G00253050 Rroxscaffold_3G00253070 Rroxscaffold_3G00253080 Rroxscaffold_3G00253130 Rroxscaffold_3G00253160 Rroxscaffold_5G00363940 Rroxscaffold_5G00381580 Rroxscaffold_6G00400360 Rroxscaffold_7G00174410
rosa_rugosa Rorug03G0121400 Rorug03G0121400 Rorug04G0175000 Rorug04G0175100 Rorug05G0180100 Rorug06G0232800 Rorug06G0232900 Rorug07G0083000 Rorug07G0083000 Rorug07G0083100 Rorug07G0083200 Rorug07G0083300 Rorug07G0083400 Rorug07G0083500 Rorug07G0083700 Rorug07G0084200 Rorug07G0084300 Rorug07G0084500 Rorug07G0084600 Rorug07G0084600 Rorug07G0084700
rosa_samantha Rh3CG189000 Rh3CG189100 Rh3CG189200 Rh3CG196500 Rh3CG196600 Rh3CG273800 Rh4DG232900 Rh5DG278900 Rh5DG279100 Rh5DG279400 Rh6CG359800 Rh7DG220500 Rh7DG220700 Rh7DG221000 Rh7DG221100 Rh7DG221600 Rh7DG221700
rosa_wichuraiana Rw0G005710 Rw0G005720 Rw3G016800 Rw4G019990 Rw5G024970 Rw5G024990 Rw6G030120 Rw7G018470 Rw7G018500 Rw7G018530 Rw7G018540 Rw7G018550 Rw7G018560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1261
AcsI RAATTY 2 cut(s) 1133, 1196
AcuI CTGAAG 6 cut(s) 128, 297, 449, 675, 834, 1194
AfaI GTAC 1 cut(s) 1066
AfiI CCNNNNNNNGG 7 cut(s) 115, 520, 568, 772, 1054, 1261, 1363
AgeI ACCGGT 1 cut(s) 765
AgsI TTSAA 6 cut(s) 312, 836, 844, 1288, 1300, 1334
AjnI CCWGG 1 cut(s) 734
AluBI AGCT 7 cut(s) 58, 320, 361, 437, 653, 880, 966
AluI AGCT 7 cut(s) 58, 320, 361, 437, 653, 880, 966
AoxI GGCC 1 cut(s) 546
ApeKI GCWGC 6 cut(s) 58, 73, 361, 494, 926, 1211
ApoI RAATTY 2 cut(s) 1133, 1196
ArsI GACNNNNNNTTYG 2 cut(s) 1181, 1213
AsiGI ACCGGT 1 cut(s) 765
Asp700I GAANNNNTTC 1 cut(s) 1047
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 6 cut(s) 76, 670, 694, 1180, 1244, 1336
AsuNHI GCTAGC 1 cut(s) 221
AvaII GGWCC 1 cut(s) 265
BbvI GCAGC 6 cut(s) 45, 85, 348, 481, 913, 1198
BccI CCATC 3 cut(s) 682, 814, 1357
BceAI ACGGC 1 cut(s) 533
BciT130I CCWGG 1 cut(s) 736
BclI TGATCA 1 cut(s) 699
BfaI CTAG 4 cut(s) 222, 477, 563, 1358
BfmI CTRYAG 3 cut(s) 201, 621, 1203
BglII AGATCT 1 cut(s) 1336
BisI GCNGC 6 cut(s) 59, 74, 362, 495, 927, 1212
BlsI GCNGC 6 cut(s) 60, 75, 363, 496, 928, 1213
Bme1390I CCNGG 1 cut(s) 736
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 2 cut(s) 213, 1059
BmrFI CCNGG 1 cut(s) 736
BmsI GCATC 1 cut(s) 1198
BmtI GCTAGC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 1 cut(s) 1037
BsaJI CCNNGG 2 cut(s) 261, 734
BsaWI WCCGGW 1 cut(s) 765
BsaXI ACNNNNNCTCC 4 cut(s) 152, 182, 463, 493
Bsc4I CCNNNNNNNGG 7 cut(s) 115, 520, 568, 772, 1054, 1261, 1363
Bse118I RCCGGY 1 cut(s) 765
Bse1I ACTGG 6 cut(s) 45, 777, 903, 909, 1155, 1196
Bse3DI GCAATG 1 cut(s) 668
BseBI CCWGG 1 cut(s) 736
BseDI CCNNGG 2 cut(s) 261, 734
BseGI GGATG 1 cut(s) 134
BseLI CCNNNNNNNGG 7 cut(s) 115, 520, 568, 772, 1054, 1261, 1363
BseMI GCAATG 1 cut(s) 668
BseMII CTCAG 3 cut(s) 605, 945, 998
BseNI ACTGG 6 cut(s) 45, 777, 903, 909, 1155, 1196
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 6 cut(s) 45, 85, 348, 481, 913, 1198
BsgI GTGCAG 1 cut(s) 92
BshFI GGCC 1 cut(s) 548
BshTI ACCGGT 1 cut(s) 765
BsiSI CCGG 2 cut(s) 766, 1304
BslI CCNNNNNNNGG 7 cut(s) 115, 520, 568, 772, 1054, 1261, 1363
BsnI GGCC 1 cut(s) 548
Bsp143I GATC 3 cut(s) 699, 799, 1336
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 3 cut(s) 606, 946, 997
BspLI GGNNCC 2 cut(s) 213, 1059
BspOI GCTAGC 1 cut(s) 225
BsrDI GCAATG 1 cut(s) 668
BsrFI RCCGGY 1 cut(s) 765
BsrI ACTGG 6 cut(s) 45, 777, 903, 909, 1155, 1196
BssAI RCCGGY 1 cut(s) 765
BssECI CCNNGG 2 cut(s) 261, 734
BssMI GATC 3 cut(s) 699, 799, 1336
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 736
Bst4CI ACNGT 2 cut(s) 715, 755
Bst6I CTCTTC 4 cut(s) 273, 646, 810, 1226
BstC8I GCNNGC 4 cut(s) 223, 306, 435, 492
BstDEI CTNAG 3 cut(s) 614, 954, 984
BstEII GGTNACC 1 cut(s) 1250
BstENI CCTNNNNNAGG 3 cut(s) 113, 566, 1052
BstF5I GGATG 1 cut(s) 134
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 3 cut(s) 702, 802, 1339
BstMBI GATC 3 cut(s) 699, 799, 1336
BstMWI GCNNNNNNNGC 3 cut(s) 58, 281, 1208
BstNI CCWGG 1 cut(s) 736
BstNSI RCATGY 1 cut(s) 242
BstPI GGTNACC 1 cut(s) 1250
BstSCI CCNGG 1 cut(s) 734
BstSFI CTRYAG 3 cut(s) 201, 621, 1203
BstV1I GCAGC 6 cut(s) 45, 85, 348, 481, 913, 1198
BstX2I RGATCY 1 cut(s) 1336
BstYI RGATCY 1 cut(s) 1336
BsuRI GGCC 1 cut(s) 548
BtsCI GGATG 1 cut(s) 134
BtsIMutI CAGTG 6 cut(s) 52, 601, 720, 896, 1113, 1148
Cac8I GCNNGC 4 cut(s) 223, 306, 435, 492
CfoI GCGC 1 cut(s) 661
Cfr10I RCCGGY 1 cut(s) 765
Cfr13I GGNCC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 1065
CspAI ACCGGT 1 cut(s) 765
CviQI GTAC 1 cut(s) 1065
DdeI CTNAG 3 cut(s) 614, 954, 984
DpnI GATC 3 cut(s) 701, 801, 1338
DpnII GATC 3 cut(s) 699, 799, 1336
Eam1104I CTCTTC 4 cut(s) 273, 646, 810, 1226
EarI CTCTTC 4 cut(s) 273, 646, 810, 1226
Eco130I CCWWGG 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 265
Eco57I CTGAAG 6 cut(s) 128, 297, 449, 675, 834, 1194
Eco91I GGTNACC 1 cut(s) 1250
EcoNI CCTNNNNNAGG 3 cut(s) 113, 566, 1052
EcoO65I GGTNACC 1 cut(s) 1250
EcoRII CCWGG 1 cut(s) 734
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FalI AAGNNNNNCTT 6 cut(s) 836, 868, 950, 982, 1061, 1093
FbaI TGATCA 1 cut(s) 699
Fnu4HI GCNGC 6 cut(s) 59, 74, 362, 495, 927, 1212
FokI GGATG 1 cut(s) 141
Fsp4HI GCNGC 6 cut(s) 59, 74, 362, 495, 927, 1212
FspBI CTAG 4 cut(s) 222, 477, 563, 1358
GlaI GCGC 1 cut(s) 660
GluI GCNGC 6 cut(s) 59, 74, 362, 495, 927, 1212
HaeIII GGCC 1 cut(s) 548
HapII CCGG 2 cut(s) 766, 1304
HhaI GCGC 1 cut(s) 661
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HincII GTYRAC 1 cut(s) 1183
HindII GTYRAC 1 cut(s) 1183
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 3 cut(s) 122, 724, 1375
HpaII CCGG 2 cut(s) 766, 1304
HphI GGTGA 6 cut(s) 76, 670, 694, 1180, 1244, 1336
Hpy166II GTNNAC 4 cut(s) 451, 671, 1113, 1183
Hpy188I TCNGA 8 cut(s) 147, 233, 429, 615, 646, 799, 973, 987
Hpy188III TCNNGA 3 cut(s) 126, 469, 812
Hpy8I GTNNAC 4 cut(s) 451, 671, 1113, 1183
HpyAV CCTTC 6 cut(s) 245, 686, 818, 1058, 1159, 1282
HpyCH4III ACNGT 2 cut(s) 715, 755
HpyCH4V TGCA 7 cut(s) 4, 73, 290, 364, 1081, 1214, 1394
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 281, 1208
HpyF3I CTNAG 3 cut(s) 614, 954, 984
HspAI GCGC 1 cut(s) 659
Ksp22I TGATCA 1 cut(s) 699
Kzo9I GATC 3 cut(s) 699, 799, 1336
LmnI GCTCC 2 cut(s) 317, 1057
Lsp1109I GCAGC 6 cut(s) 45, 85, 348, 481, 913, 1198
LweI GCATC 1 cut(s) 1198
MaeI CTAG 4 cut(s) 222, 477, 563, 1358
MalI GATC 3 cut(s) 701, 801, 1338
MboI GATC 3 cut(s) 699, 799, 1336
MboII GAAGA 8 cut(s) 290, 566, 633, 827, 1174, 1187, 1243, 1346
MfeI CAATTG 2 cut(s) 1215, 1259
MflI RGATCY 1 cut(s) 1336
MluCI AATT 8 cut(s) 674, 742, 1133, 1196, 1215, 1259, 1317, 1329
MmeI TCCRAC 1 cut(s) 292
MroXI GAANNNNTTC 1 cut(s) 1047
MseI TTAA 4 cut(s) 192, 855, 945, 1320
MslI CAYNNNNRTG 1 cut(s) 1094
MspI CCGG 2 cut(s) 766, 1304
MspR9I CCNGG 1 cut(s) 736
MunI CAATTG 2 cut(s) 1215, 1259
MvaI CCWGG 1 cut(s) 736
MwoI GCNNNNNNNGC 3 cut(s) 58, 281, 1208
NdeII GATC 3 cut(s) 699, 799, 1336
NheI GCTAGC 1 cut(s) 221
NlaIV GGNNCC 2 cut(s) 213, 1059
NmuCI GTSAC 6 cut(s) 596, 749, 910, 1085, 1168, 1250
NspI RCATGY 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 1047
PfeI GAWTC 3 cut(s) 122, 724, 1375
PflMI CCANNNNNTGG 1 cut(s) 1261
PinAI ACCGGT 1 cut(s) 765
PkrI GCNGC 6 cut(s) 60, 75, 363, 496, 928, 1213
Psp6I CCWGG 1 cut(s) 734
PspEI GGTNACC 1 cut(s) 1250
PspGI CCWGG 1 cut(s) 734
PspN4I GGNNCC 2 cut(s) 213, 1059
PspPI GGNCC 1 cut(s) 265
PsuI RGATCY 1 cut(s) 1336
RsaI GTAC 1 cut(s) 1066
RsaNI GTAC 1 cut(s) 1065
RseI CAYNNNNRTG 1 cut(s) 1094
SaqAI TTAA 4 cut(s) 192, 855, 945, 1320
SatI GCNGC 6 cut(s) 59, 74, 362, 495, 927, 1212
Sau3AI GATC 3 cut(s) 699, 799, 1336
Sau96I GGNCC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 736
SfaNI GCATC 1 cut(s) 1198
SfcI CTRYAG 3 cut(s) 201, 621, 1203
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 1094
SmlI CTYRAG 1 cut(s) 1052
SmoI CTYRAG 1 cut(s) 1052
Sse9I AATT 8 cut(s) 674, 742, 1133, 1196, 1215, 1259, 1317, 1329
SspMI CTAG 4 cut(s) 222, 477, 563, 1358
StyD4I CCNGG 1 cut(s) 734
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 2 cut(s) 715, 755
TaqI TCGA 2 cut(s) 41, 727
TasI AATT 8 cut(s) 674, 742, 1133, 1196, 1215, 1259, 1317, 1329
TatI WGTACW 1 cut(s) 1064
TfiI GAWTC 3 cut(s) 122, 724, 1375
Tru1I TTAA 4 cut(s) 192, 855, 945, 1320
Tru9I TTAA 4 cut(s) 192, 855, 945, 1320
TscAI CASTG 6 cut(s) 52, 601, 720, 903, 1120, 1155
TseFI GTSAC 6 cut(s) 596, 749, 910, 1085, 1168, 1250
TseI GCWGC 6 cut(s) 58, 73, 361, 494, 926, 1211
Tsp45I GTSAC 6 cut(s) 596, 749, 910, 1085, 1168, 1250
TspDTI ATGAA 6 cut(s) 264, 416, 1014, 1024, 1244, 1365
TspRI CASTG 6 cut(s) 52, 601, 720, 903, 1120, 1155
Van91I CCANNNNNTGG 1 cut(s) 1261
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 3 cut(s) 113, 566, 1052
XapI RAATTY 2 cut(s) 1133, 1196
XceI RCATGY 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 1047
XspI CTAG 4 cut(s) 222, 477, 563, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.