Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1073, 1551 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1454 |
| Acc36I | ACCTGC | 2 cut(s) | 997, 1676 |
| AccBSI | CCGCTC | 1 cut(s) | 2176 |
| AciI | CCGC | 4 cut(s) | 274, 953, 1133, 2176 |
| AclWI | GGATC | 6 cut(s) | 33, 62, 852, 988, 997, 2045 |
| AcoI | YGGCCR | 1 cut(s) | 1541 |
| AcsI | RAATTY | 1 cut(s) | 1292 |
| AcuI | CTGAAG | 4 cut(s) | 86, 951, 978, 1397 |
| AfaI | GTAC | 3 cut(s) | 151, 937, 1850 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 292, 2176 |
| AflII | CTTAAG | 1 cut(s) | 1475 |
| AflIII | ACRYGT | 2 cut(s) | 144, 2196 |
| AjnI | CCWGG | 3 cut(s) | 975, 1498, 2058 |
| AluBI | AGCT | 8 cut(s) | 64, 184, 739, 1255, 1309, 1642, 1770, 1792 |
| AluI | AGCT | 8 cut(s) | 64, 184, 739, 1255, 1309, 1642, 1770, 1792 |
| Alw21I | GWGCWC | 2 cut(s) | 505, 1000 |
| Alw26I | GTCTC | 1 cut(s) | 467 |
| AlwI | GGATC | 6 cut(s) | 33, 62, 852, 988, 997, 2045 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 739 |
| Ama87I | CYCGRG | 1 cut(s) | 1443 |
| AoxI | GGCC | 1 cut(s) | 1541 |
| ApeKI | GCWGC | 7 cut(s) | 271, 902, 1255, 1381, 1730, 1741, 2024 |
| ApoI | RAATTY | 1 cut(s) | 1292 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2198 |
| AspLEI | GCGC | 1 cut(s) | 2142 |
| AspS9I | GGNCC | 3 cut(s) | 892, 1009, 1348 |
| AsuC2I | CCSGG | 1 cut(s) | 425 |
| AsuHPI | GGTGA | 6 cut(s) | 49, 244, 614, 1393, 1597, 2089 |
| AvaI | CYCGRG | 1 cut(s) | 1443 |
| AvaII | GGWCC | 3 cut(s) | 892, 1009, 1348 |
| BalI | TGGCCA | 1 cut(s) | 1543 |
| BauI | CACGAG | 1 cut(s) | 2160 |
| Bbv12I | GWGCWC | 2 cut(s) | 505, 1000 |
| BbvI | GCAGC | 7 cut(s) | 258, 889, 1242, 1393, 1717, 1728, 2011 |
| BccI | CCATC | 5 cut(s) | 27, 285, 1405, 1576, 2074 |
| BcgI | CGANNNNNNTGC | 3 cut(s) | 32, 351, 385 |
| BciT130I | CCWGG | 3 cut(s) | 977, 1500, 2060 |
| BcnI | CCSGG | 1 cut(s) | 425 |
| BcoDI | GTCTC | 1 cut(s) | 467 |
| BfaI | CTAG | 3 cut(s) | 77, 852, 1013 |
| BfmI | CTRYAG | 3 cut(s) | 1224, 1297, 1755 |
| BfrI | CTTAAG | 1 cut(s) | 1475 |
| BfuAI | ACCTGC | 2 cut(s) | 997, 1676 |
| BglII | AGATCT | 2 cut(s) | 1279, 2149 |
| BisI | GCNGC | 8 cut(s) | 272, 903, 954, 1256, 1382, 1731, 1742, 2025 |
| BlsI | GCNGC | 8 cut(s) | 273, 904, 955, 1257, 1383, 1732, 1743, 2026 |
| Bme1390I | CCNGG | 4 cut(s) | 425, 977, 1500, 2060 |
| Bme18I | GGWCC | 3 cut(s) | 892, 1009, 1348 |
| BmeT110I | CYCGRG | 1 cut(s) | 1443 |
| BmgT120I | GGNCC | 3 cut(s) | 892, 1009, 1348 |
| BmiI | GGNNCC | 3 cut(s) | 894, 1666, 1869 |
| BmrFI | CCNGG | 4 cut(s) | 425, 977, 1500, 2060 |
| BmsI | GCATC | 7 cut(s) | 111, 225, 547, 632, 952, 2011, 2151 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1898, 1930 |
| Bpu10I | CCTNAGC | 1 cut(s) | 999 |
| BpuEI | CTTGAG | 4 cut(s) | 812, 1172, 1259, 2192 |
| BpuMI | CCSGG | 1 cut(s) | 425 |
| BsaAI | YACGTR | 1 cut(s) | 365 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1278 |
| BsaI | GGTCTC | 1 cut(s) | 467 |
| BsaJI | CCNNGG | 2 cut(s) | 976, 2059 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1135, 1165 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 292, 2176 |
| Bse118I | RCCGGY | 1 cut(s) | 2004 |
| Bse1I | ACTGG | 5 cut(s) | 604, 1167, 1537, 1544, 1961 |
| Bse3DI | GCAATG | 1 cut(s) | 357 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1278 |
| BseBI | CCWGG | 3 cut(s) | 977, 1500, 2060 |
| BseDI | CCNNGG | 2 cut(s) | 976, 2059 |
| BseGI | GGATG | 5 cut(s) | 38, 562, 815, 1972, 2112 |
| BseJI | GATNNNNATC | 1 cut(s) | 1278 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 292, 2176 |
| BseMI | GCAATG | 1 cut(s) | 357 |
| BseMII | CTCAG | 5 cut(s) | 110, 750, 1013, 1146, 1925 |
| BseNI | ACTGG | 5 cut(s) | 604, 1167, 1537, 1544, 1961 |
| BseXI | GCAGC | 7 cut(s) | 258, 889, 1242, 1393, 1717, 1728, 2011 |
| BseYI | CCCAGC | 1 cut(s) | 1788 |
| BshFI | GGCC | 1 cut(s) | 1543 |
| BsiHKAI | GWGCWC | 2 cut(s) | 505, 1000 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1443 |
| BsiSI | CCGG | 2 cut(s) | 424, 2005 |
| BslFI | GGGAC | 6 cut(s) | 50, 561, 710, 1460, 2115, 2192 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 292, 2176 |
| BsmAI | GTCTC | 1 cut(s) | 467 |
| BsmFI | GGGAC | 6 cut(s) | 50, 561, 710, 1460, 2115, 2192 |
| BsmI | GAATGC | 2 cut(s) | 802, 1610 |
| BsnI | GGCC | 1 cut(s) | 1543 |
| Bso31I | GGTCTC | 1 cut(s) | 467 |
| BsoBI | CYCGRG | 1 cut(s) | 1443 |
| Bsp1286I | GDGCHC | 2 cut(s) | 505, 1000 |
| BspACI | CCGC | 4 cut(s) | 274, 953, 1133, 2176 |
| BspANI | GGCC | 1 cut(s) | 1543 |
| BspCNI | CTCAG | 5 cut(s) | 109, 751, 1012, 1147, 1926 |
| BspHI | TCATGA | 1 cut(s) | 2040 |
| BspLI | GGNNCC | 3 cut(s) | 894, 1666, 1869 |
| BspMAI | CTGCAG | 1 cut(s) | 1759 |
| BspMI | ACCTGC | 2 cut(s) | 997, 1676 |
| BspPI | GGATC | 6 cut(s) | 33, 62, 852, 988, 997, 2045 |
| BspQI | GCTCTTC | 1 cut(s) | 2039 |
| BspTI | CTTAAG | 1 cut(s) | 1475 |
| BspTNI | GGTCTC | 1 cut(s) | 467 |
| BsrBI | CCGCTC | 1 cut(s) | 2176 |
| BsrDI | GCAATG | 1 cut(s) | 357 |
| BsrFI | RCCGGY | 1 cut(s) | 2004 |
| BsrI | ACTGG | 5 cut(s) | 604, 1167, 1537, 1544, 1961 |
| BssAI | RCCGGY | 1 cut(s) | 2004 |
| BssECI | CCNNGG | 2 cut(s) | 976, 2059 |
| BssSI | CACGAG | 1 cut(s) | 2160 |
| Bst2BI | CACGAG | 1 cut(s) | 2160 |
| Bst2UI | CCWGG | 3 cut(s) | 977, 1500, 2060 |
| Bst4CI | ACNGT | 7 cut(s) | 232, 1086, 1398, 1438, 1516, 1522, 2073 |
| Bst6I | CTCTTC | 4 cut(s) | 62, 1461, 1572, 2039 |
| BstAFI | CTTAAG | 1 cut(s) | 1475 |
| BstBAI | YACGTR | 1 cut(s) | 365 |
| BstC8I | GCNNGC | 3 cut(s) | 1260, 1311, 1872 |
| BstDEI | CTNAG | 8 cut(s) | 96, 245, 759, 824, 999, 1155, 1917, 1934 |
| BstF5I | GGATG | 5 cut(s) | 38, 562, 815, 1972, 2112 |
| BstHHI | GCGC | 1 cut(s) | 2142 |
| BstMAI | GTCTC | 1 cut(s) | 467 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 449, 962, 1639, 2021 |
| BstNI | CCWGG | 3 cut(s) | 977, 1500, 2060 |
| BstNSI | RCATGY | 4 cut(s) | 148, 589, 923, 2200 |
| BstSCI | CCNGG | 4 cut(s) | 423, 975, 1498, 2058 |
| BstSFI | CTRYAG | 3 cut(s) | 1224, 1297, 1755 |
| BstV1I | GCAGC | 7 cut(s) | 258, 889, 1242, 1393, 1717, 1728, 2011 |
| BstX2I | RGATCY | 4 cut(s) | 844, 989, 1279, 2149 |
| BstYI | RGATCY | 4 cut(s) | 844, 989, 1279, 2149 |
| BsuRI | GGCC | 1 cut(s) | 1543 |
| BtsCI | GGATG | 5 cut(s) | 38, 562, 815, 1972, 2112 |
| BtsI | GCAGTG | 2 cut(s) | 733, 1391 |
| BtsIMutI | CAGTG | 6 cut(s) | 733, 1082, 1276, 1391, 1443, 1968 |
| BveI | ACCTGC | 2 cut(s) | 997, 1676 |
| Cac8I | GCNNGC | 3 cut(s) | 1260, 1311, 1872 |
| CaiI | CAGNNNCTG | 1 cut(s) | 739 |
| CciI | TCATGA | 1 cut(s) | 2040 |
| CfoI | GCGC | 1 cut(s) | 2142 |
| Cfr10I | RCCGGY | 1 cut(s) | 2004 |
| Cfr13I | GGNCC | 3 cut(s) | 892, 1009, 1348 |
| Csp6I | GTAC | 3 cut(s) | 150, 936, 1849 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1177, 1212 |
| CviQI | GTAC | 3 cut(s) | 150, 936, 1849 |
| DdeI | CTNAG | 8 cut(s) | 96, 245, 759, 824, 999, 1155, 1917, 1934 |
| DraI | TTTAAA | 2 cut(s) | 913, 1101 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1454 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1454 |
| EaeI | YGGCCR | 1 cut(s) | 1541 |
| Eam1104I | CTCTTC | 4 cut(s) | 62, 1461, 1572, 2039 |
| EarI | CTCTTC | 4 cut(s) | 62, 1461, 1572, 2039 |
| Eco31I | GGTCTC | 1 cut(s) | 467 |
| Eco32I | GATATC | 1 cut(s) | 2122 |
| Eco47I | GGWCC | 3 cut(s) | 892, 1009, 1348 |
| Eco57I | CTGAAG | 4 cut(s) | 86, 951, 978, 1397 |
| Eco88I | CYCGRG | 1 cut(s) | 1443 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1009 |
| EcoRI | GAATTC | 1 cut(s) | 1292 |
| EcoRII | CCWGG | 3 cut(s) | 975, 1498, 2058 |
| EcoRV | GATATC | 1 cut(s) | 2122 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 381, 413, 1221, 1253, 1458, 1490 |
| FaqI | GGGAC | 6 cut(s) | 50, 561, 710, 1460, 2115, 2192 |
| FauI | CCCGC | 2 cut(s) | 267, 2169 |
| FauNDI | CATATG | 2 cut(s) | 166, 1054 |
| Fnu4HI | GCNGC | 8 cut(s) | 272, 903, 954, 1256, 1382, 1731, 1742, 2025 |
| FokI | GGATG | 5 cut(s) | 45, 569, 822, 1979, 2119 |
| Fsp4HI | GCNGC | 8 cut(s) | 272, 903, 954, 1256, 1382, 1731, 1742, 2025 |
| FspBI | CTAG | 3 cut(s) | 77, 852, 1013 |
| GlaI | GCGC | 1 cut(s) | 2141 |
| GluI | GCNGC | 8 cut(s) | 272, 903, 954, 1256, 1382, 1731, 1742, 2025 |
| GsaI | CCCAGC | 1 cut(s) | 1792 |
| HaeIII | GGCC | 1 cut(s) | 1543 |
| HapII | CCGG | 2 cut(s) | 424, 2005 |
| HhaI | GCGC | 1 cut(s) | 2142 |
| Hin6I | GCGC | 1 cut(s) | 2140 |
| HinP1I | GCGC | 1 cut(s) | 2140 |
| HincII | GTYRAC | 1 cut(s) | 1459 |
| HindII | GTYRAC | 1 cut(s) | 1459 |
| HindIII | AAGCTT | 2 cut(s) | 1307, 1640 |
| HinfI | GANTC | 4 cut(s) | 73, 644, 1170, 1948 |
| HpaII | CCGG | 2 cut(s) | 424, 2005 |
| HphI | GGTGA | 6 cut(s) | 49, 244, 614, 1393, 1597, 2089 |
| Hpy166II | GTNNAC | 7 cut(s) | 150, 421, 1394, 1401, 1459, 1923, 1996 |
| Hpy188I | TCNGA | 5 cut(s) | 109, 618, 1335, 1777, 2037 |
| Hpy8I | GTNNAC | 7 cut(s) | 150, 421, 1394, 1401, 1459, 1923, 1996 |
| Hpy99I | CGWCG | 1 cut(s) | 1320 |
| HpyCH4III | ACNGT | 7 cut(s) | 232, 1086, 1398, 1438, 1516, 1522, 2073 |
| HpyCH4IV | ACGT | 2 cut(s) | 364, 478 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 449, 962, 1639, 2021 |
| HpyF3I | CTNAG | 8 cut(s) | 96, 245, 759, 824, 999, 1155, 1917, 1934 |
| HpySE526I | ACGT | 2 cut(s) | 364, 478 |
| HspAI | GCGC | 1 cut(s) | 2140 |
| LguI | GCTCTTC | 1 cut(s) | 2039 |
| LmnI | GCTCC | 3 cut(s) | 799, 1003, 1867 |
| Lsp1109I | GCAGC | 7 cut(s) | 258, 889, 1242, 1393, 1717, 1728, 2011 |
| LweI | GCATC | 7 cut(s) | 111, 225, 547, 632, 952, 2011, 2151 |
| MaeI | CTAG | 3 cut(s) | 77, 852, 1013 |
| MaeII | ACGT | 2 cut(s) | 364, 478 |
| MbiI | CCGCTC | 1 cut(s) | 2176 |
| MfeI | CAATTG | 2 cut(s) | 1701, 2028 |
| MflI | RGATCY | 4 cut(s) | 844, 989, 1279, 2149 |
| MhlI | GDGCHC | 2 cut(s) | 505, 1000 |
| MlsI | TGGCCA | 1 cut(s) | 1543 |
| MluNI | TGGCCA | 1 cut(s) | 1543 |
| Mox20I | TGGCCA | 1 cut(s) | 1543 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2198 |
| MscI | TGGCCA | 1 cut(s) | 1543 |
| Msp20I | TGGCCA | 1 cut(s) | 1543 |
| MspCI | CTTAAG | 1 cut(s) | 1475 |
| MspI | CCGG | 2 cut(s) | 424, 2005 |
| MspR9I | CCNGG | 4 cut(s) | 425, 977, 1500, 2060 |
| MunI | CAATTG | 2 cut(s) | 1701, 2028 |
| Mva1269I | GAATGC | 2 cut(s) | 802, 1610 |
| MvaI | CCWGG | 3 cut(s) | 977, 1500, 2060 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 449, 962, 1639, 2021 |
| NciI | CCSGG | 1 cut(s) | 425 |
| NdeI | CATATG | 2 cut(s) | 166, 1054 |
| NlaIV | GGNNCC | 3 cut(s) | 894, 1666, 1869 |
| NmuCI | GTSAC | 4 cut(s) | 324, 602, 1516, 2162 |
| NspI | RCATGY | 4 cut(s) | 148, 589, 923, 2200 |
| PagI | TCATGA | 1 cut(s) | 2040 |
| PciI | ACATGT | 2 cut(s) | 144, 2196 |
| PciSI | GCTCTTC | 1 cut(s) | 2039 |
| PctI | GAATGC | 2 cut(s) | 802, 1610 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2198 |
| PfeI | GAWTC | 4 cut(s) | 73, 644, 1170, 1948 |
| PkrI | GCNGC | 8 cut(s) | 273, 904, 955, 1257, 1383, 1732, 1743, 2026 |
| Ppu21I | YACGTR | 1 cut(s) | 365 |
| PpuMI | RGGWCCY | 1 cut(s) | 1009 |
| PscI | ACATGT | 2 cut(s) | 144, 2196 |
| PsiI | TTATAA | 2 cut(s) | 1073, 1551 |
| Psp5II | RGGWCCY | 1 cut(s) | 1009 |
| Psp6I | CCWGG | 3 cut(s) | 975, 1498, 2058 |
| PspFI | CCCAGC | 1 cut(s) | 1788 |
| PspGI | CCWGG | 3 cut(s) | 975, 1498, 2058 |
| PspN4I | GGNNCC | 3 cut(s) | 894, 1666, 1869 |
| PspPI | GGNCC | 3 cut(s) | 892, 1009, 1348 |
| PspPPI | RGGWCCY | 1 cut(s) | 1009 |
| PstI | CTGCAG | 1 cut(s) | 1759 |
| PstNI | CAGNNNCTG | 1 cut(s) | 739 |
| PsuI | RGATCY | 4 cut(s) | 844, 989, 1279, 2149 |
| RsaI | GTAC | 3 cut(s) | 151, 937, 1850 |
| RsaNI | GTAC | 3 cut(s) | 150, 936, 1849 |
| SapI | GCTCTTC | 1 cut(s) | 2039 |
| SatI | GCNGC | 8 cut(s) | 272, 903, 954, 1256, 1382, 1731, 1742, 2025 |
| Sau96I | GGNCC | 3 cut(s) | 892, 1009, 1348 |
| ScrFI | CCNGG | 4 cut(s) | 425, 977, 1500, 2060 |
| SduI | GDGCHC | 2 cut(s) | 505, 1000 |
| SfaNI | GCATC | 7 cut(s) | 111, 225, 547, 632, 952, 2011, 2151 |
| SfcI | CTRYAG | 3 cut(s) | 1224, 1297, 1755 |
| SinI | GGWCC | 3 cut(s) | 892, 1009, 1348 |
| SmlI | CTYRAG | 5 cut(s) | 791, 1187, 1238, 1475, 2171 |
| SmoI | CTYRAG | 5 cut(s) | 791, 1187, 1238, 1475, 2171 |
| SsiI | CCGC | 4 cut(s) | 274, 953, 1133, 2176 |
| SspI | AATATT | 1 cut(s) | 1782 |
| SspMI | CTAG | 3 cut(s) | 77, 852, 1013 |
| StyD4I | CCNGG | 4 cut(s) | 423, 975, 1498, 2058 |
| TaaI | ACNGT | 7 cut(s) | 232, 1086, 1398, 1438, 1516, 1522, 2073 |
| TaiI | ACGT | 2 cut(s) | 367, 481 |
| TaqI | TCGA | 3 cut(s) | 18, 1560, 2145 |
| TatI | WGTACW | 2 cut(s) | 149, 1848 |
| TauI | GCSGC | 1 cut(s) | 956 |
| TfiI | GAWTC | 4 cut(s) | 73, 644, 1170, 1948 |
| TscAI | CASTG | 6 cut(s) | 733, 1089, 1276, 1391, 1443, 1968 |
| TseFI | GTSAC | 4 cut(s) | 324, 602, 1516, 2162 |
| TseI | GCWGC | 7 cut(s) | 271, 902, 1255, 1381, 1730, 1741, 2024 |
| Tsp45I | GTSAC | 4 cut(s) | 324, 602, 1516, 2162 |
| TspGWI | ACGGA | 2 cut(s) | 1442, 2119 |
| TspRI | CASTG | 6 cut(s) | 733, 1089, 1276, 1391, 1443, 1968 |
| Vha464I | CTTAAG | 1 cut(s) | 1475 |
| VpaK11BI | GGWCC | 3 cut(s) | 892, 1009, 1348 |
| XapI | RAATTY | 1 cut(s) | 1292 |
| XbaI | TCTAGA | 1 cut(s) | 76 |
| XceI | RCATGY | 4 cut(s) | 148, 589, 923, 2200 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 983 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2198 |
| XspI | CTAG | 3 cut(s) | 77, 852, 1013 |