Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 475 |
| AbsI | CCTCGAGG | 1 cut(s) | 27 |
| AccBSI | CCGCTC | 1 cut(s) | 1330 |
| AciI | CCGC | 2 cut(s) | 641, 1330 |
| AclWI | GGATC | 5 cut(s) | 32, 45, 1003, 1103, 1304 |
| AcoI | YGGCCR | 2 cut(s) | 594, 695 |
| AcsI | RAATTY | 2 cut(s) | 764, 839 |
| AcuI | CTGAAG | 3 cut(s) | 96, 551, 870 |
| AcyI | GRCGYC | 1 cut(s) | 472 |
| AfaI | GTAC | 4 cut(s) | 200, 809, 820, 1361 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 259, 736, 1018, 1117 |
| AflII | CTTAAG | 1 cut(s) | 341 |
| AgeI | ACCGGT | 1 cut(s) | 1114 |
| AgsI | TTSAA | 6 cut(s) | 58, 845, 904, 1066, 1267, 1279 |
| AjnI | CCWGG | 1 cut(s) | 652 |
| AluBI | AGCT | 6 cut(s) | 187, 300, 463, 702, 862, 1252 |
| AluI | AGCT | 6 cut(s) | 187, 300, 463, 702, 862, 1252 |
| Alw26I | GTCTC | 3 cut(s) | 380, 1045, 1311 |
| AlwI | GGATC | 5 cut(s) | 32, 45, 1003, 1103, 1304 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 674 |
| Ama87I | CYCGRG | 1 cut(s) | 27 |
| AoxI | GGCC | 3 cut(s) | 249, 594, 695 |
| ApeKI | GCWGC | 4 cut(s) | 61, 110, 187, 1249 |
| ApoI | RAATTY | 2 cut(s) | 764, 839 |
| AsiGI | ACCGGT | 1 cut(s) | 1114 |
| AspS9I | GGNCC | 2 cut(s) | 250, 433 |
| AvaI | CYCGRG | 1 cut(s) | 27 |
| AvaII | GGWCC | 1 cut(s) | 433 |
| BalI | TGGCCA | 2 cut(s) | 596, 697 |
| BamHI | GGATCC | 1 cut(s) | 37 |
| BbsI | GAAGAC | 1 cut(s) | 467 |
| BbvI | GCAGC | 4 cut(s) | 73, 122, 174, 1261 |
| BccI | CCATC | 3 cut(s) | 559, 730, 1178 |
| BceAI | ACGGC | 1 cut(s) | 1188 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1238, 1272 |
| BciT130I | CCWGG | 1 cut(s) | 654 |
| BcoDI | GTCTC | 3 cut(s) | 380, 1045, 1311 |
| BfaI | CTAG | 2 cut(s) | 303, 1253 |
| BfmI | CTRYAG | 1 cut(s) | 451 |
| BfrI | CTTAAG | 1 cut(s) | 341 |
| BglII | AGATCT | 1 cut(s) | 1303 |
| BisI | GCNGC | 4 cut(s) | 62, 111, 188, 1250 |
| BlsI | GCNGC | 4 cut(s) | 63, 112, 189, 1251 |
| Bme1390I | CCNGG | 1 cut(s) | 654 |
| Bme18I | GGWCC | 1 cut(s) | 433 |
| BmeT110I | CYCGRG | 1 cut(s) | 27 |
| BmgT120I | GGNCC | 2 cut(s) | 250, 433 |
| BmiI | GGNNCC | 2 cut(s) | 39, 1023 |
| BmrFI | CCNGG | 1 cut(s) | 654 |
| BmrI | ACTGGG | 1 cut(s) | 824 |
| BmsI | GCATC | 3 cut(s) | 97, 1165, 1187 |
| BmuI | ACTGGG | 1 cut(s) | 824 |
| BpiI | GAAGAC | 1 cut(s) | 467 |
| BpuEI | CTTGAG | 1 cut(s) | 413 |
| Bsa29I | ATCGAT | 1 cut(s) | 1173 |
| BsaHI | GRCGYC | 1 cut(s) | 472 |
| BsaJI | CCNNGG | 2 cut(s) | 597, 653 |
| BsaWI | WCCGGW | 1 cut(s) | 1114 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 259, 736, 1018, 1117 |
| Bse118I | RCCGGY | 1 cut(s) | 1114 |
| Bse1I | ACTGG | 2 cut(s) | 691, 819 |
| BseBI | CCWGG | 1 cut(s) | 654 |
| BseCI | ATCGAT | 1 cut(s) | 1173 |
| BseDI | CCNNGG | 2 cut(s) | 597, 653 |
| BseGI | GGATG | 2 cut(s) | 145, 810 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 259, 736, 1018, 1117 |
| BseMII | CTCAG | 4 cut(s) | 435, 911, 924, 956 |
| BseNI | ACTGG | 2 cut(s) | 691, 819 |
| BseRI | GAGGAG | 3 cut(s) | 398, 635, 1105 |
| BseXI | GCAGC | 4 cut(s) | 73, 122, 174, 1261 |
| BsgI | GTGCAG | 1 cut(s) | 327 |
| BshFI | GGCC | 3 cut(s) | 251, 596, 697 |
| BshTI | ACCGGT | 1 cut(s) | 1114 |
| BshVI | ATCGAT | 1 cut(s) | 1173 |
| BsiHKCI | CYCGRG | 1 cut(s) | 27 |
| BsiSI | CCGG | 1 cut(s) | 1115 |
| BslFI | GGGAC | 1 cut(s) | 1346 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 259, 736, 1018, 1117 |
| BsmAI | GTCTC | 3 cut(s) | 380, 1045, 1311 |
| BsmFI | GGGAC | 1 cut(s) | 1346 |
| BsnI | GGCC | 3 cut(s) | 251, 596, 697 |
| BsoBI | CYCGRG | 1 cut(s) | 27 |
| Bsp143I | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| BspACI | CCGC | 2 cut(s) | 641, 1330 |
| BspANI | GGCC | 3 cut(s) | 251, 596, 697 |
| BspCNI | CTCAG | 4 cut(s) | 436, 912, 923, 955 |
| BspDI | ATCGAT | 1 cut(s) | 1173 |
| BspLI | GGNNCC | 2 cut(s) | 39, 1023 |
| BspPI | GGATC | 5 cut(s) | 32, 45, 1003, 1103, 1304 |
| BspQI | GCTCTTC | 1 cut(s) | 1193 |
| BspTI | CTTAAG | 1 cut(s) | 341 |
| BsrBI | CCGCTC | 1 cut(s) | 1330 |
| BsrFI | RCCGGY | 1 cut(s) | 1114 |
| BsrI | ACTGG | 2 cut(s) | 691, 819 |
| BssAI | RCCGGY | 1 cut(s) | 1114 |
| BssECI | CCNNGG | 2 cut(s) | 597, 653 |
| BssMI | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| BssNI | GRCGYC | 1 cut(s) | 472 |
| BssT1I | CCWWGG | 1 cut(s) | 597 |
| Bst2UI | CCWGG | 1 cut(s) | 654 |
| Bst4CI | ACNGT | 4 cut(s) | 670, 676, 1039, 1164 |
| Bst6I | CTCTTC | 1 cut(s) | 1193 |
| BstACI | GRCGYC | 1 cut(s) | 472 |
| BstAFI | CTTAAG | 1 cut(s) | 341 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 107 |
| BstC8I | GCNNGC | 4 cut(s) | 115, 414, 465, 645 |
| BstDEI | CTNAG | 8 cut(s) | 18, 88, 444, 910, 920, 942, 1071, 1324 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 257, 1016 |
| BstF5I | GGATG | 2 cut(s) | 145, 810 |
| BstKTI | GATC | 5 cut(s) | 40, 1011, 1098, 1299, 1306 |
| BstMAI | GTCTC | 3 cut(s) | 380, 1045, 1311 |
| BstMBI | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 107, 1175 |
| BstNI | CCWGG | 1 cut(s) | 654 |
| BstNSI | RCATGY | 2 cut(s) | 68, 1264 |
| BstSCI | CCNGG | 1 cut(s) | 652 |
| BstSFI | CTRYAG | 1 cut(s) | 451 |
| BstV1I | GCAGC | 4 cut(s) | 73, 122, 174, 1261 |
| BstV2I | GAAGAC | 1 cut(s) | 467 |
| BstX2I | RGATCY | 3 cut(s) | 37, 1008, 1303 |
| BstYI | RGATCY | 3 cut(s) | 37, 1008, 1303 |
| Bsu15I | ATCGAT | 1 cut(s) | 1173 |
| BsuRI | GGCC | 3 cut(s) | 251, 596, 697 |
| BsuTUI | ATCGAT | 1 cut(s) | 1173 |
| BtsCI | GGATG | 2 cut(s) | 145, 810 |
| BtsIMutI | CAGTG | 2 cut(s) | 684, 812 |
| Cac8I | GCNNGC | 4 cut(s) | 115, 414, 465, 645 |
| CaiI | CAGNNNCTG | 1 cut(s) | 674 |
| Cfr10I | RCCGGY | 1 cut(s) | 1114 |
| Cfr13I | GGNCC | 2 cut(s) | 250, 433 |
| ClaI | ATCGAT | 1 cut(s) | 1173 |
| Csp6I | GTAC | 4 cut(s) | 199, 808, 819, 1360 |
| CspAI | ACCGGT | 1 cut(s) | 1114 |
| CviAII | CATG | 7 cut(s) | 65, 191, 506, 801, 993, 1261, 1368 |
| CviQI | GTAC | 4 cut(s) | 199, 808, 819, 1360 |
| DdeI | CTNAG | 8 cut(s) | 18, 88, 444, 910, 920, 942, 1071, 1324 |
| DpnI | GATC | 5 cut(s) | 39, 1010, 1097, 1298, 1305 |
| DpnII | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| EaeI | YGGCCR | 2 cut(s) | 594, 695 |
| Eam1104I | CTCTTC | 1 cut(s) | 1193 |
| EarI | CTCTTC | 1 cut(s) | 1193 |
| Eco130I | CCWWGG | 1 cut(s) | 597 |
| Eco47I | GGWCC | 1 cut(s) | 433 |
| Eco57I | CTGAAG | 3 cut(s) | 96, 551, 870 |
| Eco88I | CYCGRG | 1 cut(s) | 27 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 257, 1016 |
| EcoO109I | RGGNCCY | 2 cut(s) | 250, 433 |
| EcoRI | GAATTC | 1 cut(s) | 764 |
| EcoRII | CCWGG | 1 cut(s) | 652 |
| EcoT14I | CCWWGG | 1 cut(s) | 597 |
| EcoT22I | ATGCAT | 1 cut(s) | 1180 |
| ErhI | CCWWGG | 1 cut(s) | 597 |
| FaeI | CATG | 7 cut(s) | 68, 194, 509, 804, 996, 1264, 1371 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1259, 1291 |
| FaqI | GGGAC | 1 cut(s) | 1346 |
| FatI | CATG | 7 cut(s) | 64, 190, 505, 800, 992, 1260, 1367 |
| FauI | CCCGC | 1 cut(s) | 1323 |
| Fnu4HI | GCNGC | 4 cut(s) | 62, 111, 188, 1250 |
| FokI | GGATG | 2 cut(s) | 152, 817 |
| Fsp4HI | GCNGC | 4 cut(s) | 62, 111, 188, 1250 |
| FspBI | CTAG | 2 cut(s) | 303, 1253 |
| GluI | GCNGC | 4 cut(s) | 62, 111, 188, 1250 |
| HaeIII | GGCC | 3 cut(s) | 251, 596, 697 |
| HapII | CCGG | 1 cut(s) | 1115 |
| Hin1I | GRCGYC | 1 cut(s) | 472 |
| Hin1II | CATG | 7 cut(s) | 68, 194, 509, 804, 996, 1264, 1371 |
| HincII | GTYRAC | 2 cut(s) | 681, 1150 |
| HindII | GTYRAC | 2 cut(s) | 681, 1150 |
| HindIII | AAGCTT | 1 cut(s) | 461 |
| HinfI | GANTC | 2 cut(s) | 940, 1102 |
| HpaII | CCGG | 1 cut(s) | 1115 |
| Hpy166II | GTNNAC | 5 cut(s) | 555, 681, 1077, 1120, 1150 |
| Hpy188I | TCNGA | 4 cut(s) | 630, 913, 931, 974 |
| Hpy188III | TCNNGA | 1 cut(s) | 1099 |
| Hpy8I | GTNNAC | 5 cut(s) | 555, 681, 1077, 1120, 1150 |
| Hpy99I | CGWCG | 1 cut(s) | 474 |
| HpyAV | CCTTC | 3 cut(s) | 71, 118, 391 |
| HpyCH4III | ACNGT | 4 cut(s) | 670, 676, 1039, 1164 |
| HpyCH4IV | ACGT | 2 cut(s) | 210, 472 |
| HpyCH4V | TGCA | 6 cut(s) | 110, 154, 190, 279, 308, 1178 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 107, 1175 |
| HpyF3I | CTNAG | 8 cut(s) | 18, 88, 444, 910, 920, 942, 1071, 1324 |
| HpySE526I | ACGT | 2 cut(s) | 210, 472 |
| Hsp92I | GRCGYC | 1 cut(s) | 472 |
| Hsp92II | CATG | 7 cut(s) | 68, 194, 509, 804, 996, 1264, 1371 |
| Kzo9I | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| LguI | GCTCTTC | 1 cut(s) | 1193 |
| Lsp1109I | GCAGC | 4 cut(s) | 73, 122, 174, 1261 |
| LweI | GCATC | 3 cut(s) | 97, 1165, 1187 |
| MaeI | CTAG | 2 cut(s) | 303, 1253 |
| MaeII | ACGT | 2 cut(s) | 210, 472 |
| MaeIII | GTNAC | 6 cut(s) | 67, 425, 577, 670, 868, 923 |
| MalI | GATC | 5 cut(s) | 39, 1010, 1097, 1298, 1305 |
| MbiI | CCGCTC | 1 cut(s) | 1330 |
| MboI | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| MboII | GAAGA | 7 cut(s) | 116, 467, 1138, 1141, 1154, 1210, 1313 |
| MfeI | CAATTG | 1 cut(s) | 1182 |
| MflI | RGATCY | 3 cut(s) | 37, 1008, 1303 |
| MlsI | TGGCCA | 2 cut(s) | 596, 697 |
| MluCI | AATT | 4 cut(s) | 764, 839, 1182, 1348 |
| MluNI | TGGCCA | 2 cut(s) | 596, 697 |
| MlyI | GAGTC | 1 cut(s) | 934 |
| Mox20I | TGGCCA | 2 cut(s) | 596, 697 |
| Mph1103I | ATGCAT | 1 cut(s) | 1180 |
| MscI | TGGCCA | 2 cut(s) | 596, 697 |
| MseI | TTAA | 4 cut(s) | 342, 741, 947, 1014 |
| Msp20I | TGGCCA | 2 cut(s) | 596, 697 |
| MspCI | CTTAAG | 1 cut(s) | 341 |
| MspI | CCGG | 1 cut(s) | 1115 |
| MspR9I | CCNGG | 1 cut(s) | 654 |
| MunI | CAATTG | 1 cut(s) | 1182 |
| MvaI | CCWGG | 1 cut(s) | 654 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 107, 1175 |
| NdeII | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| NlaIII | CATG | 7 cut(s) | 68, 194, 509, 804, 996, 1264, 1371 |
| NlaIV | GGNNCC | 2 cut(s) | 39, 1023 |
| NmuCI | GTSAC | 2 cut(s) | 425, 868 |
| NsiI | ATGCAT | 1 cut(s) | 1180 |
| NspI | RCATGY | 2 cut(s) | 68, 1264 |
| PaeR7I | CTCGAG | 1 cut(s) | 27 |
| PciSI | GCTCTTC | 1 cut(s) | 1193 |
| PfeI | GAWTC | 1 cut(s) | 1102 |
| PinAI | ACCGGT | 1 cut(s) | 1114 |
| PkrI | GCNGC | 4 cut(s) | 63, 112, 189, 1251 |
| PleI | GAGTC | 1 cut(s) | 934 |
| PpsI | GAGTC | 1 cut(s) | 934 |
| PpuMI | RGGWCCY | 1 cut(s) | 433 |
| Psp5II | RGGWCCY | 1 cut(s) | 433 |
| Psp6I | CCWGG | 1 cut(s) | 652 |
| PspGI | CCWGG | 1 cut(s) | 652 |
| PspN4I | GGNNCC | 2 cut(s) | 39, 1023 |
| PspPI | GGNCC | 2 cut(s) | 250, 433 |
| PspPPI | RGGWCCY | 1 cut(s) | 433 |
| PspXI | VCTCGAGB | 1 cut(s) | 27 |
| PstNI | CAGNNNCTG | 1 cut(s) | 674 |
| PsuI | RGATCY | 3 cut(s) | 37, 1008, 1303 |
| RsaI | GTAC | 4 cut(s) | 200, 809, 820, 1361 |
| RsaNI | GTAC | 4 cut(s) | 199, 808, 819, 1360 |
| SapI | GCTCTTC | 1 cut(s) | 1193 |
| SaqAI | TTAA | 4 cut(s) | 342, 741, 947, 1014 |
| SatI | GCNGC | 4 cut(s) | 62, 111, 188, 1250 |
| Sau3AI | GATC | 5 cut(s) | 37, 1008, 1095, 1296, 1303 |
| Sau96I | GGNCC | 2 cut(s) | 250, 433 |
| SchI | GAGTC | 1 cut(s) | 934 |
| ScrFI | CCNGG | 1 cut(s) | 654 |
| SfaNI | GCATC | 3 cut(s) | 97, 1165, 1187 |
| SfcI | CTRYAG | 1 cut(s) | 451 |
| Sfr274I | CTCGAG | 1 cut(s) | 27 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1114 |
| SinI | GGWCC | 1 cut(s) | 433 |
| SlaI | CTCGAG | 1 cut(s) | 27 |
| SmlI | CTYRAG | 3 cut(s) | 27, 341, 392 |
| SmoI | CTYRAG | 3 cut(s) | 27, 341, 392 |
| Sse9I | AATT | 4 cut(s) | 764, 839, 1182, 1348 |
| SsiI | CCGC | 2 cut(s) | 641, 1330 |
| SspI | AATATT | 1 cut(s) | 54 |
| SspMI | CTAG | 2 cut(s) | 303, 1253 |
| StyD4I | CCNGG | 1 cut(s) | 652 |
| StyI | CCWWGG | 1 cut(s) | 597 |
| TaaI | ACNGT | 4 cut(s) | 670, 676, 1039, 1164 |
| TaiI | ACGT | 2 cut(s) | 213, 475 |
| TaqI | TCGA | 5 cut(s) | 28, 469, 1140, 1173, 1299 |
| TasI | AATT | 4 cut(s) | 764, 839, 1182, 1348 |
| TatI | WGTACW | 2 cut(s) | 198, 807 |
| TfiI | GAWTC | 1 cut(s) | 1102 |
| Tru1I | TTAA | 4 cut(s) | 342, 741, 947, 1014 |
| Tru9I | TTAA | 4 cut(s) | 342, 741, 947, 1014 |
| TscAI | CASTG | 2 cut(s) | 691, 819 |
| TseFI | GTSAC | 2 cut(s) | 425, 868 |
| TseI | GCWGC | 4 cut(s) | 61, 110, 187, 1249 |
| Tsp45I | GTSAC | 2 cut(s) | 425, 868 |
| TspDTI | ATGAA | 3 cut(s) | 231, 387, 1211 |
| TspRI | CASTG | 2 cut(s) | 691, 819 |
| Vha464I | CTTAAG | 1 cut(s) | 341 |
| VpaK11BI | GGWCC | 1 cut(s) | 433 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 257, 1016 |
| XapI | RAATTY | 2 cut(s) | 764, 839 |
| XceI | RCATGY | 2 cut(s) | 68, 1264 |
| XhoI | CTCGAG | 1 cut(s) | 27 |
| XspI | CTAG | 2 cut(s) | 303, 1253 |
| ZraI | GACGTC | 1 cut(s) | 473 |
| Zsp2I | ATGCAT | 1 cut(s) | 1180 |