FvH4_6g19530

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013943
Physical Location & Seq
Reverse (-)
13044393 .. 13045931
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19530.t1

Sequence Viewer

Length: 1032 bp
ATGGCTCACTCCGCCCCCCAACCACCGTCAACCACCGTCGATGAAAACCCCTCCGCCTCGATCTCCGCCGTCCATCCCGACATAATTCAAGCTCACATCCTCACCCGCCTCGACGGCCCCACTCTGGCCTCCGCCGCCTGCACCTCCTCCCAGCTCCACTCCCTCGCCTCCGACCACCGCCTCTGGTCCAACGTCTGCCACTCCACTTGGCCATCCACCGCCGCGCCACGTCTCCGCCACCTCATCTCCTCCTTCCCCGACGGCGCCTTCTCTTTCTTCTCCGACTCCTTCCCCCTCCTCGCCAACCTAGACTCCTCCTCTGCCGCCGCAGCCGATCATCACGACCGTCCGAGTGAGCTGATCTCCGCCGTCGACATCTACCACGGCGAGAAGCTCATCTTCAGCAAGGTCGTGGAGACGGAGACGGTGAGCGGCTGGTTCATGTGCTCGCCCTTCCGGGTTGACCTGCTGGACCCCAAGGACGTGGTGCCGACGTCGGTGAAGTACCCGGAGGCTGACACGTGTCGCAACCTGGGGGAGGAGCTGCGGCTGAGCTGGATCCTGATCGACCCGGCGGGACGGCGAGCCATGAACCTCTCGAGCCACACGGCGGTTTCGGTGCAGCGCCACTGGCTGAGCGGTGAGGTGCACGCGCGGTTCGCGTCAATTCTCCCCGGCGAGAAAGGATCGGCGTCGGAGTACGTACAGTTAGGGATCGTGGTGACGTGCAGGGTTGGGTCGGAGGGAGGGGAGCTGCAGGTGAGGGAGGTGAGCCTGCAGGTGGAGGACATGGATGGGATGCATTTGAACGGGAAGGAGAGTTTGGTAATTTTGCAGAAGGGGTTGGAGGGAAGGAAAGGGAGGAAGGGGAGGAGGGAGGAGGAAGGGAGGAGGAGATACGAAGAGTACTTGGAGAGGAAGAGGGAAAGGAAGGAGAAGAAGATGAGGACAGAAGGGACATTGGATACTTTGTGTGTCACTTTTGGGGTCTTGGGGTTTTTGCTTTTTTGGTTATTCGTCTTGTGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

344

Amino Acids

38.06

Weight (kDa)

6.28

Isoelectric Point (pI)

47.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 26 - 67 2.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36090
fragaria_vesca FvH4_6g19530
malus_domestica MD12G1031600.v1.1 MD14G1031900.v1.1
prunus_persica Prupe.7G100900_v2.0.a1
pyrus_communis pycom12g02940 pycom14g02880
rosa_chinensis RchiOBHm_Chr3g0474871
rosa_laevigata RLG00000023886
rosa_multiflora Rmu_sc0011900.1_g000011
rosa_roxburghii Rroxscaffold_6G00406780
rosa_rugosa Rorug03G0145100
rosa_samantha Rh3AG194400 Rh3BG224300 Rh3CG219200 Rh3DG220100
rosa_wichuraiana Rw3G017700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 748, 769
AatII GACGTC 1 cut(s) 497
Acc36I ACCTGC 3 cut(s) 474, 748, 769
AccB1I GGYRCC 2 cut(s) 263, 487
AccBSI CCGCTC 2 cut(s) 432, 639
AccI GTMKAC 1 cut(s) 372
AccII CGCG 4 cut(s) 224, 653, 655, 662
AclWI GGATC 4 cut(s) 553, 566, 694, 722
AcoI YGGCCR 1 cut(s) 209
AcuI CTGAAG 1 cut(s) 385
AcvI CACGTG 1 cut(s) 522
AcyI GRCGYC 3 cut(s) 264, 494, 692
AfaI GTAC 4 cut(s) 506, 701, 705, 908
AfiI CCNNNNNNNGG 4 cut(s) 124, 538, 610, 781
AflIII ACRYGT 2 cut(s) 519, 521
AgsI TTSAA 2 cut(s) 89, 808
AjiI CACGTC 3 cut(s) 230, 484, 726
AjnI CCWGG 1 cut(s) 531
AjuI GAANNNNNNNTTGG 2 cut(s) 806, 838
AluBI AGCT 7 cut(s) 92, 154, 358, 394, 544, 555, 754
AluI AGCT 7 cut(s) 92, 154, 358, 394, 544, 555, 754
Alw21I GWGCWC 2 cut(s) 449, 651
Alw26I GTCTC 3 cut(s) 236, 410, 416
Alw44I GTGCAC 1 cut(s) 647
AlwI GGATC 4 cut(s) 553, 566, 694, 722
Ama87I CYCGRG 1 cut(s) 598
AoxI GGCC 3 cut(s) 115, 126, 209
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 4 cut(s) 329, 544, 622, 754
AspLEI GCGC 4 cut(s) 226, 266, 627, 655
AspS9I GGNCC 3 cut(s) 116, 186, 472
AsuC2I CCSGG 4 cut(s) 458, 509, 572, 675
AsuHPI GGTGA 7 cut(s) 94, 439, 511, 653, 733, 772, 781
AvaI CYCGRG 1 cut(s) 598
AvaII GGWCC 2 cut(s) 186, 472
BaeGI GKGCMC 1 cut(s) 651
BalI TGGCCA 1 cut(s) 211
BamHI GGATCC 1 cut(s) 558
BanI GGYRCC 2 cut(s) 263, 487
BbrPI CACGTG 1 cut(s) 522
Bbv12I GWGCWC 2 cut(s) 449, 651
BbvI GCAGC 4 cut(s) 341, 531, 634, 741
BccI CCATC 3 cut(s) 81, 220, 788
BceAI ACGGC 7 cut(s) 53, 130, 277, 353, 400, 596, 624
BciT130I CCWGG 1 cut(s) 533
BciVI GTATCC 1 cut(s) 958
BcnI CCSGG 4 cut(s) 458, 509, 572, 675
BcoDI GTCTC 3 cut(s) 236, 410, 416
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 2 cut(s) 755, 776
BfoI RGCGCY 2 cut(s) 267, 628
BfuAI ACCTGC 3 cut(s) 474, 748, 769
BfuI GTATCC 1 cut(s) 958
BglI GCCNNNNNGGC 1 cut(s) 114
BlpI GCTNAGC 2 cut(s) 551, 635
BmcAI AGTACT 1 cut(s) 908
Bme1390I CCNGG 5 cut(s) 458, 509, 533, 572, 675
Bme18I GGWCC 2 cut(s) 186, 472
BmeT110I CYCGRG 1 cut(s) 598
BmgBI CACGTC 3 cut(s) 230, 484, 726
BmgT120I GGNCC 3 cut(s) 116, 186, 472
BmiI GGNNCC 5 cut(s) 118, 265, 474, 489, 560
BmrFI CCNGG 5 cut(s) 458, 509, 533, 572, 675
BmsI GCATC 1 cut(s) 789
BoxI GACNNNNGTC 1 cut(s) 522
BplI GAGNNNNNCTC 2 cut(s) 347, 379
Bpu1102I GCTNAGC 2 cut(s) 551, 635
BpuMI CCSGG 4 cut(s) 458, 509, 572, 675
BsaAI YACGTR 2 cut(s) 522, 703
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 3 cut(s) 264, 494, 692
BsaJI CCNNGG 4 cut(s) 382, 477, 532, 673
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 296, 326
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 538, 610, 781
Bse1I ACTGG 1 cut(s) 635
Bse8I GATNNNNATC 1 cut(s) 563
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 4 cut(s) 382, 477, 532, 673
BseGI GGATG 5 cut(s) 73, 96, 212, 799, 804
BseJI GATNNNNATC 1 cut(s) 563
BseLI CCNNNNNNNGG 4 cut(s) 124, 538, 610, 781
BseMII CTCAG 2 cut(s) 542, 626
BseNI ACTGG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 4 cut(s) 341, 531, 634, 741
BseYI CCCAGC 1 cut(s) 150
BsgI GTGCAG 3 cut(s) 124, 641, 748
Bsh1236I CGCG 4 cut(s) 224, 653, 655, 662
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 3 cut(s) 117, 128, 211
BshNI GGYRCC 2 cut(s) 263, 487
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 449, 651
BsiHKCI CYCGRG 1 cut(s) 598
BsiSI CCGG 4 cut(s) 457, 509, 572, 675
BslFI GGGAC 2 cut(s) 591, 970
BslI CCNNNNNNNGG 4 cut(s) 124, 538, 610, 781
BsmAI GTCTC 3 cut(s) 236, 410, 416
BsmBI CGTCTC 3 cut(s) 236, 410, 416
BsmFI GGGAC 2 cut(s) 591, 970
BsnI GGCC 3 cut(s) 117, 128, 211
BsoBI CYCGRG 1 cut(s) 598
Bsp1286I GDGCHC 2 cut(s) 449, 651
Bsp143I GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
Bsp1720I GCTNAGC 2 cut(s) 551, 635
BspANI GGCC 3 cut(s) 117, 128, 211
BspCNI CTCAG 2 cut(s) 543, 627
BspFNI CGCG 4 cut(s) 224, 653, 655, 662
BspLI GGNNCC 5 cut(s) 118, 265, 474, 489, 560
BspMAI CTGCAG 2 cut(s) 759, 780
BspMI ACCTGC 3 cut(s) 474, 748, 769
BspPI GGATC 4 cut(s) 553, 566, 694, 722
BspT107I GGYRCC 2 cut(s) 263, 487
BsrBI CCGCTC 2 cut(s) 432, 639
BsrI ACTGG 1 cut(s) 635
BssECI CCNNGG 4 cut(s) 382, 477, 532, 673
BssMI GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
BssNI GRCGYC 3 cut(s) 264, 494, 692
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 5 cut(s) 27, 37, 347, 427, 708
Bst6I CTCTTC 2 cut(s) 897, 914
BstACI GRCGYC 3 cut(s) 264, 494, 692
BstBAI YACGTR 2 cut(s) 522, 703
BstC8I GCNNGC 5 cut(s) 139, 449, 585, 651, 776
BstDEI CTNAG 2 cut(s) 551, 635
BstDSI CCRYGG 1 cut(s) 382
BstENI CCTNNNNNAGG 1 cut(s) 536
BstF5I GGATG 5 cut(s) 73, 96, 212, 799, 804
BstFNI CGCG 4 cut(s) 224, 653, 655, 662
BstH2I RGCGCY 2 cut(s) 267, 628
BstHHI GCGC 4 cut(s) 226, 266, 627, 655
BstKTI GATC 7 cut(s) 63, 337, 363, 561, 567, 689, 717
BstMAI GTCTC 3 cut(s) 236, 410, 416
BstMBI GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 6 cut(s) 11, 114, 134, 329, 631, 659
BstNI CCWGG 1 cut(s) 533
BstPAI GACNNNNGTC 1 cut(s) 522
BstSCI CCNGG 5 cut(s) 456, 507, 531, 570, 673
BstSFI CTRYAG 2 cut(s) 755, 776
BstSLI GKGCMC 1 cut(s) 651
BstSNI TACGTA 1 cut(s) 703
BstUI CGCG 4 cut(s) 224, 653, 655, 662
BstV1I GCAGC 4 cut(s) 341, 531, 634, 741
BstX2I RGATCY 1 cut(s) 558
BstXI CCANNNNNNTGG 1 cut(s) 484
BstYI RGATCY 1 cut(s) 558
BsuI GTATCC 1 cut(s) 958
BsuRI GGCC 3 cut(s) 117, 128, 211
BtgI CCRYGG 1 cut(s) 382
BtrI CACGTC 3 cut(s) 230, 484, 726
BtsCI GGATG 5 cut(s) 73, 96, 212, 799, 804
BtsIMutI CAGTG 1 cut(s) 628
BveI ACCTGC 3 cut(s) 474, 748, 769
Cac8I GCNNGC 5 cut(s) 139, 449, 585, 651, 776
CfoI GCGC 4 cut(s) 226, 266, 627, 655
Cfr13I GGNCC 3 cut(s) 116, 186, 472
CseI GACGC 2 cut(s) 651, 681
Csp6I GTAC 4 cut(s) 505, 700, 704, 907
CspCI CAANNNNNGTGG 2 cut(s) 188, 223
CviAII CATG 3 cut(s) 442, 589, 790
CviQI GTAC 4 cut(s) 505, 700, 704, 907
DdeI CTNAG 2 cut(s) 551, 635
DinI GGCGCC 1 cut(s) 265
DpnI GATC 7 cut(s) 62, 336, 362, 560, 566, 688, 716
DpnII GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 897, 914
EarI CTCTTC 2 cut(s) 897, 914
EciI GGCGGA 5 cut(s) 43, 55, 121, 224, 355
Eco105I TACGTA 1 cut(s) 703
Eco130I CCWWGG 1 cut(s) 477
Eco47I GGWCC 2 cut(s) 186, 472
Eco57I CTGAAG 1 cut(s) 385
Eco72I CACGTG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 804
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 477
Esp3I CGTCTC 3 cut(s) 236, 410, 416
FaeI CATG 3 cut(s) 445, 592, 793
FaiI YATR 4 cut(s) 83, 443, 590, 791
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FaqI GGGAC 2 cut(s) 591, 970
FatI CATG 3 cut(s) 441, 588, 789
FauI CCCGC 2 cut(s) 113, 568
FblI GTMKAC 1 cut(s) 372
FokI GGATG 5 cut(s) 60, 83, 199, 806, 811
FspBI CTAG 1 cut(s) 308
GlaI GCGC 4 cut(s) 225, 265, 626, 654
GsaI CCCAGC 1 cut(s) 154
HaeII RGCGCY 2 cut(s) 267, 628
HaeIII GGCC 3 cut(s) 117, 128, 211
HapII CCGG 4 cut(s) 457, 509, 572, 675
HgaI GACGC 2 cut(s) 651, 681
HhaI GCGC 4 cut(s) 226, 266, 627, 655
Hin1I GRCGYC 3 cut(s) 264, 494, 692
Hin1II CATG 3 cut(s) 445, 592, 793
Hin6I GCGC 4 cut(s) 224, 264, 625, 653
HinP1I GCGC 4 cut(s) 224, 264, 625, 653
HincII GTYRAC 3 cut(s) 30, 373, 463
HindII GTYRAC 3 cut(s) 30, 373, 463
HinfI GANTC 2 cut(s) 284, 311
HpaII CCGG 4 cut(s) 457, 509, 572, 675
HphI GGTGA 7 cut(s) 94, 439, 511, 653, 733, 772, 781
Hpy166II GTNNAC 4 cut(s) 30, 373, 463, 649
Hpy188I TCNGA 5 cut(s) 172, 283, 351, 697, 742
Hpy188III TCNNGA 4 cut(s) 77, 341, 562, 598
Hpy8I GTNNAC 4 cut(s) 30, 373, 463, 649
Hpy99I CGWCG 7 cut(s) 41, 116, 263, 374, 496, 499, 697
HpyCH4III ACNGT 5 cut(s) 27, 37, 347, 427, 708
HpyCH4IV ACGT 7 cut(s) 192, 229, 483, 494, 521, 702, 725
HpyCH4V TGCA 9 cut(s) 141, 622, 649, 729, 757, 778, 802, 835, 1026
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 114, 134, 329, 631, 659
HpyF3I CTNAG 2 cut(s) 551, 635
HpySE526I ACGT 7 cut(s) 192, 229, 483, 494, 521, 702, 725
Hsp92I GRCGYC 3 cut(s) 264, 494, 692
Hsp92II CATG 3 cut(s) 445, 592, 793
HspAI GCGC 4 cut(s) 224, 264, 625, 653
KasI GGCGCC 1 cut(s) 263
Kzo9I GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
LmnI GCTCC 3 cut(s) 159, 541, 751
Lsp1109I GCAGC 4 cut(s) 341, 531, 634, 741
LweI GCATC 1 cut(s) 789
MaeI CTAG 1 cut(s) 308
MaeII ACGT 7 cut(s) 192, 229, 483, 494, 521, 702, 725
MaeIII GTNAC 2 cut(s) 721, 976
MalI GATC 7 cut(s) 62, 336, 362, 560, 566, 688, 716
MbiI CCGCTC 2 cut(s) 432, 639
MboI GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
MboII GAAGA 6 cut(s) 268, 391, 914, 931, 949, 952
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 2 cut(s) 449, 651
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 3 cut(s) 84, 666, 828
MluNI TGGCCA 1 cut(s) 211
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 2 cut(s) 278, 305
MmeI TCCRAC 6 cut(s) 195, 213, 306, 675, 720, 825
Mox20I TGGCCA 1 cut(s) 211
Mph1103I ATGCAT 1 cut(s) 804
MscI TGGCCA 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 211
MspI CCGG 4 cut(s) 457, 509, 572, 675
MspR9I CCNGG 5 cut(s) 458, 509, 533, 572, 675
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 4 cut(s) 224, 653, 655, 662
MwoI GCNNNNNNNGC 6 cut(s) 11, 114, 134, 329, 631, 659
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 4 cut(s) 458, 509, 572, 675
NdeII GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
NlaIII CATG 3 cut(s) 445, 592, 793
NlaIV GGNNCC 5 cut(s) 118, 265, 474, 489, 560
NmuCI GTSAC 2 cut(s) 721, 976
NsiI ATGCAT 1 cut(s) 804
PaeR7I CTCGAG 1 cut(s) 598
PaqCI CACCTGC 2 cut(s) 748, 769
PleI GAGTC 2 cut(s) 278, 305
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 522
PmlI CACGTG 1 cut(s) 522
PpsI GAGTC 2 cut(s) 278, 305
Ppu21I YACGTR 2 cut(s) 522, 703
PshAI GACNNNNGTC 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 531
PspCI CACGTG 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 5 cut(s) 118, 265, 474, 489, 560
PspPI GGNCC 3 cut(s) 116, 186, 472
PstI CTGCAG 2 cut(s) 759, 780
PsuI RGATCY 1 cut(s) 558
RsaI GTAC 4 cut(s) 506, 701, 705, 908
RsaNI GTAC 4 cut(s) 505, 700, 704, 907
SalI GTCGAC 1 cut(s) 371
Sau3AI GATC 7 cut(s) 60, 334, 360, 558, 564, 686, 714
Sau96I GGNCC 3 cut(s) 116, 186, 472
SbfI CCTGCAGG 1 cut(s) 780
ScaI AGTACT 1 cut(s) 908
SchI GAGTC 2 cut(s) 278, 305
ScrFI CCNGG 5 cut(s) 458, 509, 533, 572, 675
SdaI CCTGCAGG 1 cut(s) 780
SduI GDGCHC 2 cut(s) 449, 651
SfaNI GCATC 1 cut(s) 789
SfcI CTRYAG 2 cut(s) 755, 776
SfoI GGCGCC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 598
SinI GGWCC 2 cut(s) 186, 472
SlaI CTCGAG 1 cut(s) 598
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
SnaBI TACGTA 1 cut(s) 703
Sse8387I CCTGCAGG 1 cut(s) 780
Sse9I AATT 3 cut(s) 84, 666, 828
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 5 cut(s) 456, 507, 531, 570, 673
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 5 cut(s) 27, 37, 347, 427, 708
TaiI ACGT 7 cut(s) 195, 232, 486, 497, 524, 705, 728
TaqI TCGA 6 cut(s) 39, 59, 111, 372, 567, 599
TasI AATT 3 cut(s) 84, 666, 828
TatI WGTACW 1 cut(s) 906
TauI GCSGC 6 cut(s) 137, 224, 326, 329, 435, 550
TscAI CASTG 1 cut(s) 635
TseFI GTSAC 2 cut(s) 721, 976
TseI GCWGC 4 cut(s) 329, 544, 622, 754
Tsp45I GTSAC 2 cut(s) 721, 976
TspDTI ATGAA 3 cut(s) 57, 430, 605
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 1 cut(s) 635
VneI GTGCAC 1 cut(s) 647
VpaK11BI GGWCC 2 cut(s) 186, 472
XagI CCTNNNNNAGG 1 cut(s) 536
XhoI CTCGAG 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 1 cut(s) 308
ZraI GACGTC 1 cut(s) 495
ZrmI AGTACT 1 cut(s) 908
Zsp2I ATGCAT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.