MD12G1031600.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013943
Physical Location & Seq
Reverse (-)
3468287 .. 3469348
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1031600.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGCCTGCTCCTCCCACCTACCGCCGTTCGCCGCCGCCAACGAAGCCAGCTCCGCCTCAATCTCCGCCGTCCATCCGGACATAATCCAAACCCACATCCTCACCCGCCTCGACGCCCCCACGCTAGCCTCCGCCGCCTGCACCTCCTCCGAACTCCACGCGCTGGCCTCCCACCAGCTCCTCTGGGCAAACATCTGCTACTCCACATGGCCCTCCATCACAACGCCACGTGTCCGCCAGGTCATCTCCACGTTCCCCGACGGGCCCATCTCCTTCTTCTCCGACTCCTTCCCGCTCCTCGGCAATCTGAACCCCACCACCACAGCCGCAACCTCCTCTGGTCTCGCGCCAAAACACCATCAGGACCATCCGTACGAATTAATCTCTGCGGTCGATATCTACCACCGCGGGAAGCTGATCCTGAGCAAGGTCATCGAGACGGAGACCGTGACCGGGTGGTTCCGGTGCTCGCCGTTCCGGATTGACCTGCTGGACCCCAAGGACGTGGTCCCCACCCATATCAAGTACCAGGAGGGGCTGGAAGCCGACACGTGTCGCGAACTCGGGGACGATCTCACGCTGAGCTGGATCCTTATCGACCCTACCGGACGTCGGGCGTTGAACCTCTCCAGTCACGCGCCGCTGTCGGTGAAGCGGCACTGGCTGAGCGGTGAGTTGCACGTACAGTTCGCGTCGATTCTGGCCGGGCAGAAGGGGACGGCGTCGGAGCACGTGCTGTGCGGGATATTGGTCACGTGCGGGGGATCGGAGGGAGGGGAGATGCAGGTGAGGGAGGTGAGCTTGCAGGTGGAGGACATGGATGGAATGCATCTGAATGGGAGGGAGGGTTTGGTAATTCTGGATAGGGCGTTGGAGGGCAGAAGGGGAAGAAAGGGGAGGAGGAGGGTGGGGGAAGGAAAGAAGAGGTACGAAGAGTATTTGCAGGGGAAGAAGGAAAGGAAGGAGAGGAAGCTGAGGACTGAAGGGACATTAGATACTTTGTGTGTGGCTTTTGGGGTATTTGGATTTTTCATTTTTTGGTCATATATTTTGTGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

354

Amino Acids

39.02

Weight (kDa)

6.94

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 26 - 67 1.5e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36090
fragaria_vesca FvH4_6g19530
malus_domestica MD12G1031600.v1.1 MD14G1031900.v1.1
prunus_persica Prupe.7G100900_v2.0.a1
pyrus_communis pycom12g02940 pycom14g02880
rosa_chinensis RchiOBHm_Chr3g0474871
rosa_laevigata RLG00000023886
rosa_multiflora Rmu_sc0011900.1_g000011
rosa_roxburghii Rroxscaffold_6G00406780
rosa_rugosa Rorug03G0145100
rosa_samantha Rh3AG194400 Rh3BG224300 Rh3CG219200 Rh3DG220100
rosa_wichuraiana Rw3G017700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 775, 796
AatII GACGTC 1 cut(s) 613
Acc36I ACCTGC 3 cut(s) 495, 775, 796
AccB7I CCANNNNNTGG 1 cut(s) 163
AccBSI CCGCTC 2 cut(s) 295, 669
AccII CGCG 6 cut(s) 161, 347, 408, 558, 638, 692
AccIII TCCGGA 2 cut(s) 76, 477
AclWI GGATC 4 cut(s) 412, 583, 596, 772
AcoI YGGCCR 1 cut(s) 702
AcuI CTGAAG 1 cut(s) 1002
AcvI CACGTG 4 cut(s) 230, 552, 733, 756
AcyI GRCGYC 3 cut(s) 114, 610, 722
AfaI GTAC 4 cut(s) 374, 527, 684, 929
AfiI CCNNNNNNNGG 5 cut(s) 163, 299, 427, 453, 612
AflIII ACRYGT 3 cut(s) 229, 549, 551
AgsI TTSAA 1 cut(s) 622
AjiI CACGTC 1 cut(s) 505
AjnI CCWGG 2 cut(s) 237, 528
AluBI AGCT 6 cut(s) 51, 178, 415, 585, 801, 973
AluI AGCT 6 cut(s) 51, 178, 415, 585, 801, 973
Alw21I GWGCWC 2 cut(s) 470, 732
Alw26I GTCTC 3 cut(s) 347, 431, 437
AlwI GGATC 4 cut(s) 412, 583, 596, 772
Ama87I CYCGRG 1 cut(s) 563
Aor13HI TCCGGA 2 cut(s) 76, 477
AoxI GGCC 5 cut(s) 3, 165, 209, 263, 702
ApaI GGGCCC 1 cut(s) 267
AseI ATTAAT 1 cut(s) 380
AspLEI GCGC 3 cut(s) 163, 349, 640
AspS9I GGNCC 6 cut(s) 210, 263, 264, 364, 493, 508
AsuC2I CCSGG 2 cut(s) 454, 706
AsuHPI GGTGA 5 cut(s) 94, 661, 683, 799, 808
AsuNHI GCTAGC 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 3 cut(s) 364, 493, 508
BaeGI GKGCMC 1 cut(s) 267
BamHI GGATCC 1 cut(s) 588
BanII GRGCYC 1 cut(s) 267
BbrPI CACGTG 4 cut(s) 230, 552, 733, 756
Bbv12I GWGCWC 2 cut(s) 470, 732
BbvCI CCTCAGC 1 cut(s) 974
BccI CCATC 6 cut(s) 81, 224, 275, 366, 375, 815
BceAI ACGGC 4 cut(s) 10, 53, 457, 735
BcgI CGANNNNNNTGC 2 cut(s) 415, 449
BciT130I CCWGG 2 cut(s) 239, 530
BcnI CCSGG 2 cut(s) 454, 706
BcoDI GTCTC 3 cut(s) 347, 431, 437
BfaI CTAG 1 cut(s) 125
BfuAI ACCTGC 3 cut(s) 495, 775, 796
BisI GCNGC 6 cut(s) 33, 36, 135, 327, 641, 656
BlpI GCTNAGC 2 cut(s) 581, 665
BlsI GCNGC 6 cut(s) 34, 37, 136, 328, 642, 657
Bme1390I CCNGG 4 cut(s) 239, 454, 530, 706
Bme18I GGWCC 3 cut(s) 364, 493, 508
BmeT110I CYCGRG 1 cut(s) 563
BmgBI CACGTC 1 cut(s) 505
BmgT120I GGNCC 6 cut(s) 210, 263, 264, 364, 493, 508
BmiI GGNNCC 5 cut(s) 265, 461, 495, 510, 590
BmrFI CCNGG 4 cut(s) 239, 454, 530, 706
BmsI GCATC 2 cut(s) 771, 838
BmtI GCTAGC 1 cut(s) 128
BoxI GACNNNNGTC 1 cut(s) 552
BpmI CTGGAG 1 cut(s) 613
Bpu10I CCTNAGC 2 cut(s) 422, 974
Bpu1102I GCTNAGC 2 cut(s) 581, 665
BpuMI CCSGG 2 cut(s) 454, 706
BsaAI YACGTR 5 cut(s) 230, 552, 682, 733, 756
BsaBI GATNNNNATC 1 cut(s) 593
BsaHI GRCGYC 3 cut(s) 114, 610, 722
BsaI GGTCTC 2 cut(s) 347, 437
BsaJI CCNNGG 3 cut(s) 298, 406, 498
BsaWI WCCGGW 4 cut(s) 76, 462, 477, 605
BsaXI ACNNNNNCTCC 2 cut(s) 832, 862
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 299, 427, 453, 612
Bse1I ACTGG 2 cut(s) 630, 665
Bse8I GATNNNNATC 1 cut(s) 593
BseAI TCCGGA 2 cut(s) 76, 477
BseBI CCWGG 2 cut(s) 239, 530
BseDI CCNNGG 3 cut(s) 298, 406, 498
BseGI GGATG 4 cut(s) 73, 96, 367, 826
BseJI GATNNNNATC 1 cut(s) 593
BseLI CCNNNNNNNGG 5 cut(s) 163, 299, 427, 453, 612
BseMII CTCAG 4 cut(s) 413, 572, 656, 965
BseNI ACTGG 2 cut(s) 630, 665
BseRI GAGGAG 6 cut(s) 136, 170, 287, 325, 913, 916
BseSI GKGCMC 1 cut(s) 267
BsgI GTGCAG 1 cut(s) 124
Bsh1236I CGCG 6 cut(s) 161, 347, 408, 558, 638, 692
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 5 cut(s) 5, 167, 211, 265, 704
BsiEI CGRYCG 1 cut(s) 393
BsiHKAI GWGCWC 2 cut(s) 470, 732
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 6 cut(s) 77, 453, 463, 478, 606, 705
BsiWI CGTACG 1 cut(s) 372
BslFI GGGAC 4 cut(s) 494, 581, 730, 1000
BslI CCNNNNNNNGG 5 cut(s) 163, 299, 427, 453, 612
BsmAI GTCTC 3 cut(s) 347, 431, 437
BsmBI CGTCTC 1 cut(s) 431
BsmFI GGGAC 4 cut(s) 494, 581, 730, 1000
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 5 cut(s) 5, 167, 211, 265, 704
Bso31I GGTCTC 2 cut(s) 347, 437
BsoBI CYCGRG 1 cut(s) 563
Bsp120I GGGCCC 1 cut(s) 263
Bsp1286I GDGCHC 3 cut(s) 267, 470, 732
Bsp13I TCCGGA 2 cut(s) 76, 477
Bsp143I GATC 4 cut(s) 417, 571, 588, 764
Bsp1720I GCTNAGC 2 cut(s) 581, 665
Bsp68I TCGCGA 1 cut(s) 558
BspANI GGCC 5 cut(s) 5, 167, 211, 265, 704
BspCNI CTCAG 4 cut(s) 414, 573, 657, 966
BspEI TCCGGA 2 cut(s) 76, 477
BspFNI CGCG 6 cut(s) 161, 347, 408, 558, 638, 692
BspLI GGNNCC 5 cut(s) 265, 461, 495, 510, 590
BspMI ACCTGC 3 cut(s) 495, 775, 796
BspOI GCTAGC 1 cut(s) 128
BspPI GGATC 4 cut(s) 412, 583, 596, 772
BspTNI GGTCTC 2 cut(s) 347, 437
BsrBI CCGCTC 2 cut(s) 295, 669
BsrI ACTGG 2 cut(s) 630, 665
BssECI CCNNGG 3 cut(s) 298, 406, 498
BssMI GATC 4 cut(s) 417, 571, 588, 764
BssNI GRCGYC 3 cut(s) 114, 610, 722
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 2 cut(s) 239, 530
Bst4CI ACNGT 2 cut(s) 448, 687
Bst6I CTCTTC 2 cut(s) 917, 927
BstACI GRCGYC 3 cut(s) 114, 610, 722
BstBAI YACGTR 5 cut(s) 230, 552, 682, 733, 756
BstC8I GCNNGC 7 cut(s) 7, 49, 126, 139, 165, 470, 803
BstDEI CTNAG 4 cut(s) 422, 581, 665, 974
BstDSI CCRYGG 1 cut(s) 406
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 4 cut(s) 73, 96, 367, 826
BstFNI CGCG 6 cut(s) 161, 347, 408, 558, 638, 692
BstHHI GCGC 3 cut(s) 163, 349, 640
BstKTI GATC 4 cut(s) 420, 574, 591, 767
BstMAI GTCTC 3 cut(s) 347, 431, 437
BstMBI GATC 4 cut(s) 417, 571, 588, 764
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 134, 661
BstNI CCWGG 2 cut(s) 239, 530
BstPAI GACNNNNGTC 1 cut(s) 552
BstSCI CCNGG 4 cut(s) 237, 452, 528, 704
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 6 cut(s) 161, 347, 408, 558, 638, 692
BstX2I RGATCY 1 cut(s) 588
BstXI CCANNNNNNTGG 1 cut(s) 505
BstYI RGATCY 1 cut(s) 588
BsuRI GGCC 5 cut(s) 5, 167, 211, 265, 704
BtgI CCRYGG 1 cut(s) 406
BtrI CACGTC 1 cut(s) 505
BtsCI GGATG 4 cut(s) 73, 96, 367, 826
BtsIMutI CAGTG 1 cut(s) 658
BtuMI TCGCGA 1 cut(s) 558
BveI ACCTGC 3 cut(s) 495, 775, 796
Cac8I GCNNGC 7 cut(s) 7, 49, 126, 139, 165, 470, 803
CfoI GCGC 3 cut(s) 163, 349, 640
Cfr13I GGNCC 6 cut(s) 210, 263, 264, 364, 493, 508
Cfr42I CCGCGG 1 cut(s) 409
CseI GACGC 3 cut(s) 122, 681, 711
Csp6I GTAC 4 cut(s) 373, 526, 683, 928
CviAII CATG 2 cut(s) 207, 817
CviQI GTAC 4 cut(s) 373, 526, 683, 928
DdeI CTNAG 4 cut(s) 422, 581, 665, 974
DpnI GATC 4 cut(s) 419, 573, 590, 766
DpnII GATC 4 cut(s) 417, 571, 588, 764
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 2 cut(s) 917, 927
EarI CTCTTC 2 cut(s) 917, 927
EciI GGCGGA 4 cut(s) 43, 55, 121, 224
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 347, 437
Eco32I GATATC 1 cut(s) 397
Eco47I GGWCC 3 cut(s) 364, 493, 508
Eco57I CTGAAG 1 cut(s) 1002
Eco72I CACGTG 4 cut(s) 230, 552, 733, 756
Eco88I CYCGRG 1 cut(s) 563
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 237, 528
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 831
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 498
Esp3I CGTCTC 1 cut(s) 431
FaeI CATG 2 cut(s) 210, 820
FaiI YATR 6 cut(s) 83, 208, 519, 818, 1045, 1047
FaqI GGGAC 4 cut(s) 494, 581, 730, 1000
FatI CATG 2 cut(s) 206, 816
FauI CCCGC 5 cut(s) 113, 300, 401, 734, 752
Fnu4HI GCNGC 6 cut(s) 33, 36, 135, 327, 641, 656
FokI GGATG 4 cut(s) 60, 83, 354, 833
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 6 cut(s) 33, 36, 135, 327, 641, 656
FspBI CTAG 1 cut(s) 125
GlaI GCGC 3 cut(s) 162, 348, 639
GluI GCNGC 6 cut(s) 33, 36, 135, 327, 641, 656
GsuI CTGGAG 1 cut(s) 613
HaeIII GGCC 5 cut(s) 5, 167, 211, 265, 704
HapII CCGG 6 cut(s) 77, 453, 463, 478, 606, 705
HgaI GACGC 3 cut(s) 122, 681, 711
HhaI GCGC 3 cut(s) 163, 349, 640
Hin1I GRCGYC 3 cut(s) 114, 610, 722
Hin1II CATG 2 cut(s) 210, 820
Hin6I GCGC 3 cut(s) 161, 347, 638
HinP1I GCGC 3 cut(s) 161, 347, 638
HinfI GANTC 2 cut(s) 284, 697
HpaII CCGG 6 cut(s) 77, 453, 463, 478, 606, 705
HphI GGTGA 5 cut(s) 94, 661, 683, 799, 808
Hpy188I TCNGA 6 cut(s) 151, 283, 309, 727, 769, 834
Hpy188III TCNNGA 7 cut(s) 77, 362, 421, 436, 478, 557, 860
Hpy99I CGWCG 5 cut(s) 116, 263, 615, 697, 727
HpyAV CCTTC 8 cut(s) 283, 298, 706, 876, 908, 946, 955, 977
HpyCH4III ACNGT 2 cut(s) 448, 687
HpyCH4IV ACGT 8 cut(s) 229, 251, 504, 551, 610, 681, 732, 755
HpyCH4V TGCA 7 cut(s) 141, 679, 784, 805, 829, 943, 1056
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 134, 661
HpyF3I CTNAG 4 cut(s) 422, 581, 665, 974
HpySE526I ACGT 8 cut(s) 229, 251, 504, 551, 610, 681, 732, 755
Hsp92I GRCGYC 3 cut(s) 114, 610, 722
Hsp92II CATG 2 cut(s) 210, 820
HspAI GCGC 3 cut(s) 161, 347, 638
Kpn2I TCCGGA 2 cut(s) 76, 477
KspI CCGCGG 1 cut(s) 409
Kzo9I GATC 4 cut(s) 417, 571, 588, 764
LmnI GCTCC 5 cut(s) 14, 56, 183, 300, 727
LweI GCATC 2 cut(s) 771, 838
MaeI CTAG 1 cut(s) 125
MaeII ACGT 8 cut(s) 229, 251, 504, 551, 610, 681, 732, 755
MaeIII GTNAC 3 cut(s) 448, 632, 751
MalI GATC 4 cut(s) 419, 573, 590, 766
MbiI CCGCTC 2 cut(s) 295, 669
MboI GATC 4 cut(s) 417, 571, 588, 764
MboII GAAGA 5 cut(s) 268, 900, 934, 944, 961
MflI RGATCY 1 cut(s) 588
MhlI GDGCHC 3 cut(s) 267, 470, 732
MluCI AATT 2 cut(s) 377, 855
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 3 cut(s) 306, 705, 852
Mph1103I ATGCAT 1 cut(s) 831
MroI TCCGGA 2 cut(s) 76, 477
MseI TTAA 1 cut(s) 380
MslI CAYNNNNRTG 1 cut(s) 834
MspA1I CMGCKG 2 cut(s) 408, 643
MspI CCGG 6 cut(s) 77, 453, 463, 478, 606, 705
MspR9I CCNGG 4 cut(s) 239, 454, 530, 706
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 2 cut(s) 239, 530
MvnI CGCG 6 cut(s) 161, 347, 408, 558, 638, 692
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 134, 661
NciI CCSGG 2 cut(s) 454, 706
NdeII GATC 4 cut(s) 417, 571, 588, 764
NheI GCTAGC 1 cut(s) 124
NlaIII CATG 2 cut(s) 210, 820
NlaIV GGNNCC 5 cut(s) 265, 461, 495, 510, 590
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 3 cut(s) 448, 632, 751
NruI TCGCGA 1 cut(s) 558
NsiI ATGCAT 1 cut(s) 831
PaqCI CACCTGC 2 cut(s) 775, 796
PctI GAATGC 1 cut(s) 831
PfeI GAWTC 1 cut(s) 697
Pfl23II CGTACG 1 cut(s) 372
PflFI GACNNNGTC 2 cut(s) 506, 721
PflMI CCANNNNNTGG 1 cut(s) 163
PkrI GCNGC 6 cut(s) 34, 37, 136, 328, 642, 657
PleI GAGTC 1 cut(s) 278
PmaCI CACGTG 4 cut(s) 230, 552, 733, 756
PmlI CACGTG 4 cut(s) 230, 552, 733, 756
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 5 cut(s) 230, 552, 682, 733, 756
PshAI GACNNNNGTC 1 cut(s) 552
PshBI ATTAAT 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 237, 528
PspCI CACGTG 4 cut(s) 230, 552, 733, 756
PspGI CCWGG 2 cut(s) 237, 528
PspLI CGTACG 1 cut(s) 372
PspN4I GGNNCC 5 cut(s) 265, 461, 495, 510, 590
PspOMI GGGCCC 1 cut(s) 263
PspPI GGNCC 6 cut(s) 210, 263, 264, 364, 493, 508
PsuI RGATCY 1 cut(s) 588
PsyI GACNNNGTC 2 cut(s) 506, 721
RruI TCGCGA 1 cut(s) 558
RsaI GTAC 4 cut(s) 374, 527, 684, 929
RsaNI GTAC 4 cut(s) 373, 526, 683, 928
RseI CAYNNNNRTG 1 cut(s) 834
SacII CCGCGG 1 cut(s) 409
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 6 cut(s) 33, 36, 135, 327, 641, 656
Sau3AI GATC 4 cut(s) 417, 571, 588, 764
Sau96I GGNCC 6 cut(s) 210, 263, 264, 364, 493, 508
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 4 cut(s) 239, 454, 530, 706
SduI GDGCHC 3 cut(s) 267, 470, 732
SfaNI GCATC 2 cut(s) 771, 838
Sfr303I CCGCGG 1 cut(s) 409
SgrBI CCGCGG 1 cut(s) 409
SinI GGWCC 3 cut(s) 364, 493, 508
SmiMI CAYNNNNRTG 1 cut(s) 834
Sse9I AATT 2 cut(s) 377, 855
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 4 cut(s) 237, 452, 528, 704
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 448, 687
TaiI ACGT 8 cut(s) 232, 254, 507, 554, 613, 684, 735, 758
TaqI TCGA 5 cut(s) 111, 393, 435, 597, 695
TasI AATT 2 cut(s) 377, 855
TauI GCSGC 6 cut(s) 35, 38, 137, 329, 643, 658
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TscAI CASTG 1 cut(s) 665
TseFI GTSAC 3 cut(s) 448, 632, 751
Tsp45I GTSAC 3 cut(s) 448, 632, 751
TspDTI ATGAA 1 cut(s) 1021
TspGWI ACGGA 2 cut(s) 360, 455
TspRI CASTG 1 cut(s) 665
Tth111I GACNNNGTC 2 cut(s) 506, 721
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 3 cut(s) 364, 493, 508
VspI ATTAAT 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 425
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 611
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.