RLG00000023886

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013943
Physical Location & Seq
Forward (+)
29577664 .. 29579130
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023886

Sequence Viewer

Length: 1038 bp
ATGGCTCGCTCCTCCCACCAACCTCTGTCAACCACCGTCGACGAAGACTCCTCCGCTTCGATCTCCGCCGTCCATCCCGACATAATTCAAACTCACATCCTCACCCGCCTCGACGGCCCCACTCTCGCCTCCGCCGCCTGCACCTCCTCCCAGCTCCACACCCTCGCCTCCGACCACCGCATCTGGGCCAACATCTGCCACTCCACGTGGCCCTCCACCACAACGCCACGTCTCCGCCAAGTCATCTCCACCTTCCCCGACGGCGCCTTCTCTTTCTTCTCCGACTCCTTCCCGCTTCTCGCCAACTTAGACTCCGCCGCAGCCGCCGATCATCACGATAGTCCGACTGAGCTAATCTCCGCTGTCGATATCTACCACGGTGACAAGCTCATCTTCAGCAAGGTGGTGGAGACGGAGACTGTGAGCGGATGGTTCACATGCTCGCCGTTCCGGATTGACCTGCTGGACCCCAAGGACGTGGTTCCGACGCCGGTGAAGTACCCGGTGGGGCAGGAGGCCGACACGTGTCGCGATCTGGGGGAGGAACTGAGGCTGAGCTGGATTCTGATCGACCCGGTGGGACGGCGCGCCATGAACCTCTCGAGCCACACGGCGGTTTCGGTGCAGCGGCACTGGCTGAGCGGTGAGGTGCACGCGCGGTTCGCTTCGATTCTTCCCGGCCAGAGAGGGACGGCGTCGGAGTTCGTGCAGTGCGGGATCTTGGTGACGTGCGGCGCTGGGTCGGAGGGAGGGGAGTTGCAGGTGAGGGAGGTGAGCCTGCAGGTGGAGGACATGGACGGAATGCATTTGAACGGGAAGGACGGTTTGGTAATTTTGCAGAGGGGGTTGGAGGGCAGAAAGGGGAGGAAGGTGGGGAGGAGAGGGGAGGAGGGAAGGAGGAGATACGGAGACTACTTGGAGAGGAAGAGGGAAAGGAAGGAGAAGAAGATGAGGACAGAAGGGACATTAGACACTTTGTGTGTCACTTTTGGCGTATTGGGGTTTTTGCTTTTTTGGTTATTTATTTTGTGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

346

Amino Acids

38.14

Weight (kDa)

6.23

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 26 - 67 1.2e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36090
fragaria_vesca FvH4_6g19530
malus_domestica MD12G1031600.v1.1 MD14G1031900.v1.1
prunus_persica Prupe.7G100900_v2.0.a1
pyrus_communis pycom12g02940 pycom14g02880
rosa_chinensis RchiOBHm_Chr3g0474871
rosa_laevigata RLG00000023886
rosa_multiflora Rmu_sc0011900.1_g000011
rosa_roxburghii Rroxscaffold_6G00406780
rosa_rugosa Rorug03G0145100
rosa_samantha Rh3AG194400 Rh3BG224300 Rh3CG219200 Rh3DG220100
rosa_wichuraiana Rw3G017700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 751, 772
Acc36I ACCTGC 3 cut(s) 468, 751, 772
AccB1I GGYRCC 1 cut(s) 263
AccBSI CCGCTC 2 cut(s) 426, 642
AccI GTMKAC 1 cut(s) 39
AccII CGCG 4 cut(s) 531, 588, 656, 658
AccIII TCCGGA 1 cut(s) 450
AclWI GGATC 1 cut(s) 725
AcoI YGGCCR 1 cut(s) 679
AcuI CTGAAG 1 cut(s) 379
AcvI CACGTG 2 cut(s) 207, 525
AcyI GRCGYC 3 cut(s) 264, 488, 695
AdeI CACNNNGTG 1 cut(s) 978
AfaI GTAC 1 cut(s) 500
AfiI CCNNNNNNNGG 3 cut(s) 184, 613, 784
AflIII ACRYGT 2 cut(s) 522, 524
AgsI TTSAA 2 cut(s) 89, 811
AjiI CACGTC 3 cut(s) 230, 478, 729
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AluBI AGCT 4 cut(s) 154, 352, 388, 558
AluI AGCT 4 cut(s) 154, 352, 388, 558
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 4 cut(s) 236, 404, 410, 903
Alw44I GTGCAC 1 cut(s) 650
AlwI GGATC 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 601
Aor13HI TCCGGA 1 cut(s) 450
AoxI GGCC 5 cut(s) 115, 186, 209, 516, 679
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 2 cut(s) 320, 625
AscI GGCGCGCC 1 cut(s) 586
AspLEI GCGC 5 cut(s) 266, 588, 590, 658, 737
AspS9I GGNCC 4 cut(s) 116, 186, 210, 466
AsuC2I CCSGG 3 cut(s) 503, 575, 678
AsuHPI GGTGA 7 cut(s) 94, 392, 505, 656, 736, 775, 784
AvaI CYCGRG 1 cut(s) 601
AvaII GGWCC 1 cut(s) 466
BaeGI GKGCMC 1 cut(s) 654
BanI GGYRCC 1 cut(s) 263
BbrPI CACGTG 2 cut(s) 207, 525
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 2 cut(s) 332, 637
BccI CCATC 2 cut(s) 81, 423
BceAI ACGGC 7 cut(s) 53, 130, 277, 430, 599, 627, 708
BcnI CCSGG 3 cut(s) 503, 575, 678
BcoDI GTCTC 4 cut(s) 236, 404, 410, 903
BfmI CTRYAG 1 cut(s) 779
BfoI RGCGCY 2 cut(s) 267, 738
BfuAI ACCTGC 3 cut(s) 468, 751, 772
BglI GCCNNNNNGGC 1 cut(s) 114
BisI GCNGC 7 cut(s) 135, 318, 321, 324, 626, 629, 733
BlpI GCTNAGC 2 cut(s) 554, 638
BlsI GCNGC 7 cut(s) 136, 319, 322, 325, 627, 630, 734
Bme1390I CCNGG 3 cut(s) 503, 575, 678
Bme18I GGWCC 1 cut(s) 466
BmeT110I CYCGRG 1 cut(s) 601
BmgBI CACGTC 3 cut(s) 230, 478, 729
BmgT120I GGNCC 4 cut(s) 116, 186, 210, 466
BmiI GGNNCC 4 cut(s) 118, 265, 468, 483
BmrFI CCNGG 3 cut(s) 503, 575, 678
BmsI GCATC 1 cut(s) 189
BoxI GACNNNNGTC 1 cut(s) 525
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 2 cut(s) 341, 373
Bpu1102I GCTNAGC 2 cut(s) 554, 638
BpuMI CCSGG 3 cut(s) 503, 575, 678
BsaAI YACGTR 2 cut(s) 207, 525
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 3 cut(s) 264, 488, 695
BsaJI CCNNGG 2 cut(s) 376, 471
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 296, 326
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 613, 784
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 1 cut(s) 638
Bse8I GATNNNNATC 1 cut(s) 566
BseAI TCCGGA 1 cut(s) 450
BseDI CCNNGG 2 cut(s) 376, 471
BseGI GGATG 3 cut(s) 73, 96, 434
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 3 cut(s) 184, 613, 784
BseMII CTCAG 4 cut(s) 339, 539, 545, 629
BseNI ACTGG 1 cut(s) 638
BsePI GCGCGC 1 cut(s) 586
BseRI GAGGAG 5 cut(s) 40, 136, 892, 902, 913
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 2 cut(s) 332, 637
BseYI CCCAGC 2 cut(s) 150, 737
BsgI GTGCAG 3 cut(s) 124, 644, 728
Bsh1236I CGCG 4 cut(s) 531, 588, 656, 658
BshFI GGCC 5 cut(s) 117, 188, 211, 518, 681
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 654
BsiHKCI CYCGRG 1 cut(s) 601
BsiSI CCGG 5 cut(s) 451, 491, 503, 575, 678
BslFI GGGAC 3 cut(s) 594, 703, 976
BslI CCNNNNNNNGG 3 cut(s) 184, 613, 784
BsmAI GTCTC 4 cut(s) 236, 404, 410, 903
BsmBI CGTCTC 2 cut(s) 236, 404
BsmFI GGGAC 3 cut(s) 594, 703, 976
BsmI GAATGC 1 cut(s) 807
BsnI GGCC 5 cut(s) 117, 188, 211, 518, 681
BsoBI CYCGRG 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 654
Bsp13I TCCGGA 1 cut(s) 450
Bsp143I GATC 5 cut(s) 60, 328, 532, 567, 717
Bsp1720I GCTNAGC 2 cut(s) 554, 638
Bsp68I TCGCGA 1 cut(s) 531
BspANI GGCC 5 cut(s) 117, 188, 211, 518, 681
BspCNI CTCAG 4 cut(s) 340, 540, 546, 630
BspEI TCCGGA 1 cut(s) 450
BspFNI CGCG 4 cut(s) 531, 588, 656, 658
BspLI GGNNCC 4 cut(s) 118, 265, 468, 483
BspMAI CTGCAG 1 cut(s) 783
BspMI ACCTGC 3 cut(s) 468, 751, 772
BspPI GGATC 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 263
BsrBI CCGCTC 2 cut(s) 426, 642
BsrFI RCCGGY 1 cut(s) 490
BsrI ACTGG 1 cut(s) 638
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 376, 471
BssHII GCGCGC 1 cut(s) 586
BssMI GATC 5 cut(s) 60, 328, 532, 567, 717
BssNI GRCGYC 3 cut(s) 264, 488, 695
BssT1I CCWWGG 1 cut(s) 471
Bst4CI ACNGT 4 cut(s) 37, 380, 421, 824
Bst6I CTCTTC 1 cut(s) 920
BstACI GRCGYC 3 cut(s) 264, 488, 695
BstBAI YACGTR 2 cut(s) 207, 525
BstC8I GCNNGC 6 cut(s) 7, 139, 443, 588, 654, 779
BstDEI CTNAG 5 cut(s) 307, 348, 548, 554, 638
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 3 cut(s) 73, 96, 434
BstFNI CGCG 4 cut(s) 531, 588, 656, 658
BstH2I RGCGCY 2 cut(s) 267, 738
BstHHI GCGC 5 cut(s) 266, 588, 590, 658, 737
BstKTI GATC 5 cut(s) 63, 331, 535, 570, 720
BstMAI GTCTC 4 cut(s) 236, 404, 410, 903
BstMBI GATC 5 cut(s) 60, 328, 532, 567, 717
BstMWI GCNNNNNNNGC 5 cut(s) 114, 134, 323, 634, 662
BstNSI RCATGY 1 cut(s) 441
BstPAI GACNNNNGTC 1 cut(s) 525
BstSCI CCNGG 3 cut(s) 501, 573, 676
BstSFI CTRYAG 1 cut(s) 779
BstSLI GKGCMC 1 cut(s) 654
BstUI CGCG 4 cut(s) 531, 588, 656, 658
BstV1I GCAGC 2 cut(s) 332, 637
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 717
BstXI CCANNNNNNTGG 1 cut(s) 478
BstYI RGATCY 1 cut(s) 717
BsuRI GGCC 5 cut(s) 117, 188, 211, 518, 681
BtgI CCRYGG 1 cut(s) 376
BtrI CACGTC 3 cut(s) 230, 478, 729
BtsCI GGATG 3 cut(s) 73, 96, 434
BtsI GCAGTG 1 cut(s) 716
BtsIMutI CAGTG 2 cut(s) 631, 716
BtuMI TCGCGA 1 cut(s) 531
BveI ACCTGC 3 cut(s) 468, 751, 772
Cac8I GCNNGC 6 cut(s) 7, 139, 443, 588, 654, 779
CfoI GCGC 5 cut(s) 266, 588, 590, 658, 737
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 4 cut(s) 116, 186, 210, 466
CseI GACGC 2 cut(s) 496, 684
Csp6I GTAC 1 cut(s) 499
CviAII CATG 3 cut(s) 438, 592, 793
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 5 cut(s) 307, 348, 548, 554, 638
DinI GGCGCC 1 cut(s) 265
DpnI GATC 5 cut(s) 62, 330, 534, 569, 719
DpnII GATC 5 cut(s) 60, 328, 532, 567, 717
DraIII CACNNNGTG 1 cut(s) 978
EaeI YGGCCR 1 cut(s) 679
Eam1104I CTCTTC 1 cut(s) 920
EarI CTCTTC 1 cut(s) 920
EciI GGCGGA 4 cut(s) 55, 121, 224, 304
Eco130I CCWWGG 1 cut(s) 471
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 379
Eco72I CACGTG 2 cut(s) 207, 525
Eco88I CYCGRG 1 cut(s) 601
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 807
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 471
Esp3I CGTCTC 2 cut(s) 236, 404
FaeI CATG 3 cut(s) 441, 595, 796
FaiI YATR 4 cut(s) 83, 439, 593, 794
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 3 cut(s) 594, 703, 976
FatI CATG 3 cut(s) 437, 591, 792
FauI CCCGC 3 cut(s) 113, 300, 707
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 7 cut(s) 135, 318, 321, 324, 626, 629, 733
FokI GGATG 3 cut(s) 60, 83, 441
Fsp4HI GCNGC 7 cut(s) 135, 318, 321, 324, 626, 629, 733
GlaI GCGC 5 cut(s) 265, 587, 589, 657, 736
GluI GCNGC 7 cut(s) 135, 318, 321, 324, 626, 629, 733
GsaI CCCAGC 2 cut(s) 154, 741
HaeII RGCGCY 2 cut(s) 267, 738
HaeIII GGCC 5 cut(s) 117, 188, 211, 518, 681
HapII CCGG 5 cut(s) 451, 491, 503, 575, 678
HgaI GACGC 2 cut(s) 496, 684
HhaI GCGC 5 cut(s) 266, 588, 590, 658, 737
Hin1I GRCGYC 3 cut(s) 264, 488, 695
Hin1II CATG 3 cut(s) 441, 595, 796
Hin6I GCGC 5 cut(s) 264, 586, 588, 656, 735
HinP1I GCGC 5 cut(s) 264, 586, 588, 656, 735
HincII GTYRAC 2 cut(s) 30, 40
HindII GTYRAC 2 cut(s) 30, 40
HinfI GANTC 5 cut(s) 47, 284, 311, 562, 670
HpaII CCGG 5 cut(s) 451, 491, 503, 575, 678
HphI GGTGA 7 cut(s) 94, 392, 505, 656, 736, 775, 784
Hpy166II GTNNAC 4 cut(s) 30, 40, 435, 652
Hpy188I TCNGA 7 cut(s) 172, 283, 345, 486, 567, 700, 745
Hpy188III TCNNGA 5 cut(s) 77, 335, 451, 530, 601
Hpy8I GTNNAC 4 cut(s) 30, 40, 435, 652
Hpy99I CGWCG 6 cut(s) 41, 44, 116, 263, 490, 700
HpyAV CCTTC 8 cut(s) 262, 277, 298, 811, 862, 888, 931, 953
HpyCH4III ACNGT 4 cut(s) 37, 380, 421, 824
HpyCH4IV ACGT 5 cut(s) 206, 229, 477, 524, 728
HpyCH4V TGCA 9 cut(s) 141, 625, 652, 709, 760, 781, 805, 838, 1032
HpyF10VI GCNNNNNNNGC 5 cut(s) 114, 134, 323, 634, 662
HpyF3I CTNAG 5 cut(s) 307, 348, 548, 554, 638
HpySE526I ACGT 5 cut(s) 206, 229, 477, 524, 728
Hsp92I GRCGYC 3 cut(s) 264, 488, 695
Hsp92II CATG 3 cut(s) 441, 595, 796
HspAI GCGC 5 cut(s) 264, 586, 588, 656, 735
KasI GGCGCC 1 cut(s) 263
Kpn2I TCCGGA 1 cut(s) 450
Kzo9I GATC 5 cut(s) 60, 328, 532, 567, 717
LmnI GCTCC 2 cut(s) 14, 159
Lsp1109I GCAGC 2 cut(s) 332, 637
LweI GCATC 1 cut(s) 189
MaeII ACGT 5 cut(s) 206, 229, 477, 524, 728
MaeIII GTNAC 3 cut(s) 380, 724, 982
MalI GATC 5 cut(s) 62, 330, 534, 569, 719
MbiI CCGCTC 2 cut(s) 426, 642
MboI GATC 5 cut(s) 60, 328, 532, 567, 717
MboII GAAGA 7 cut(s) 56, 268, 385, 665, 937, 955, 958
MflI RGATCY 1 cut(s) 717
MhlI GDGCHC 1 cut(s) 654
MluCI AATT 2 cut(s) 84, 831
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 3 cut(s) 41, 278, 305
MmeI TCCRAC 7 cut(s) 195, 306, 368, 509, 678, 723, 828
Mph1103I ATGCAT 1 cut(s) 807
MroI TCCGGA 1 cut(s) 450
MspA1I CMGCKG 2 cut(s) 362, 628
MspI CCGG 5 cut(s) 451, 491, 503, 575, 678
MspR9I CCNGG 3 cut(s) 503, 575, 678
Mva1269I GAATGC 1 cut(s) 807
MvnI CGCG 4 cut(s) 531, 588, 656, 658
MwoI GCNNNNNNNGC 5 cut(s) 114, 134, 323, 634, 662
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 3 cut(s) 503, 575, 678
NdeII GATC 5 cut(s) 60, 328, 532, 567, 717
NlaIII CATG 3 cut(s) 441, 595, 796
NlaIV GGNNCC 4 cut(s) 118, 265, 468, 483
NmuCI GTSAC 3 cut(s) 380, 724, 982
NruI TCGCGA 1 cut(s) 531
NsiI ATGCAT 1 cut(s) 807
NspI RCATGY 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 601
PalAI GGCGCGCC 1 cut(s) 586
PaqCI CACCTGC 2 cut(s) 751, 772
PauI GCGCGC 1 cut(s) 586
PctI GAATGC 1 cut(s) 807
PfeI GAWTC 2 cut(s) 562, 670
PflFI GACNNNGTC 1 cut(s) 694
PkrI GCNGC 7 cut(s) 136, 319, 322, 325, 627, 630, 734
PleI GAGTC 3 cut(s) 41, 278, 305
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 2 cut(s) 207, 525
PmlI CACGTG 2 cut(s) 207, 525
PpsI GAGTC 3 cut(s) 41, 278, 305
Ppu21I YACGTR 2 cut(s) 207, 525
PshAI GACNNNNGTC 1 cut(s) 525
PspCI CACGTG 2 cut(s) 207, 525
PspFI CCCAGC 2 cut(s) 150, 737
PspN4I GGNNCC 4 cut(s) 118, 265, 468, 483
PspPI GGNCC 4 cut(s) 116, 186, 210, 466
PstI CTGCAG 1 cut(s) 783
PsuI RGATCY 1 cut(s) 717
PsyI GACNNNGTC 1 cut(s) 694
PteI GCGCGC 1 cut(s) 586
RruI TCGCGA 1 cut(s) 531
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
SalI GTCGAC 1 cut(s) 38
SatI GCNGC 7 cut(s) 135, 318, 321, 324, 626, 629, 733
Sau3AI GATC 5 cut(s) 60, 328, 532, 567, 717
Sau96I GGNCC 4 cut(s) 116, 186, 210, 466
SbfI CCTGCAGG 1 cut(s) 783
SchI GAGTC 3 cut(s) 41, 278, 305
ScrFI CCNGG 3 cut(s) 503, 575, 678
SdaI CCTGCAGG 1 cut(s) 783
SduI GDGCHC 1 cut(s) 654
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 779
SfoI GGCGCC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 601
SgrAI CRCCGGYG 1 cut(s) 490
SgrDI CGTCGACG 1 cut(s) 38
SgsI GGCGCGCC 1 cut(s) 586
SinI GGWCC 1 cut(s) 466
SlaI CTCGAG 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 601
SmoI CTYRAG 1 cut(s) 601
Sse8387I CCTGCAGG 1 cut(s) 783
Sse9I AATT 2 cut(s) 84, 831
SspDI GGCGCC 1 cut(s) 263
StyD4I CCNGG 3 cut(s) 501, 573, 676
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 4 cut(s) 37, 380, 421, 824
TaiI ACGT 5 cut(s) 209, 232, 480, 527, 731
TaqI TCGA 7 cut(s) 39, 59, 111, 366, 570, 602, 668
TasI AATT 2 cut(s) 84, 831
TauI GCSGC 5 cut(s) 137, 320, 326, 631, 735
TfiI GAWTC 2 cut(s) 562, 670
TscAI CASTG 2 cut(s) 638, 716
TseFI GTSAC 3 cut(s) 380, 724, 982
TseI GCWGC 2 cut(s) 320, 625
Tsp45I GTSAC 3 cut(s) 380, 724, 982
TspDTI ATGAA 1 cut(s) 608
TspGWI ACGGA 3 cut(s) 428, 813, 921
TspRI CASTG 2 cut(s) 638, 716
Tth111I GACNNNGTC 1 cut(s) 694
VneI GTGCAC 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 466
XceI RCATGY 1 cut(s) 441
XhoI CTCGAG 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 39
Zsp2I ATGCAT 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.