FvH4_6g19710

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015657
Physical Location & Seq
Reverse (-)
13172603 .. 13175014
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19710.t1

Sequence Viewer

Length: 1323 bp
ATGGAGTGGCTGCATATCGTGGTGTTATTATCGACGTTAGCAGTTCATGTATCATGGGGTCTTGAAGGCACTACTGGTTCGCTACCTGAGCTGAGTAAACTGAGTCAGGAGTTGCTGGACTCGGCAAGGGATCCCGAATTCTTTGATTGGTTGAGAAAAGTCAGGAGGAAAATCCACGAGAACCCTGAGCTCGCCTTTGAGGAGGAAGAGACGAGCCGATTCATAAGATCGGAGCTCGACTTGCTTGGGATAGAGTACATATGGCCGGTGGCTAAGACTGGAGTGGTTGCCTCTATCGGTTCCGGGGTGCAGCCGCGGTTCGCTCTCAGGGCAGACATGGATGCTCTCCCAATTCAGGAATTGGTTGAGTGGGAGCACAAGAGCAAGAACCCTGGGAAGATGCATGCTTGTGGCCATGATGTTCATGTAACCATGCTCCTTGGAGCGGCTAAGTTACTTCAGCTAAAGAGTAAAGAACTGAAGGGAACAGTCAAGCTGGTTTTTCAACCTGCAGAGGAGGGTCATGCCGGGGCATATCATATGTTAAAAGAGGGTGCTCTTGATGGAATTGAAGGAATATTTGGGCTACACGTTTGGCCAGAACAGCCGACTGGTTCTATCGGTTCTAGACCTGGTCCATTCCTTGCTGGTGCTGGTAGATTTTTAGTCACCGTCCAAGGGCAAGGTGGACATGCTGCAGCTCCCCATTTGACTGCTGACCCAATTCTTGCAGCCTGCTCGATCATCATTGCGCTCCAACAGATCGTATCTCGAGAAACCGACCCTCTAGAGGCCAGGGTGGTAACTGTTGGGTTTGTTAACGGTGGCCAAGCCGCAAATGTCATCCCAGAAACAGTGGAACTTAAAGGGACTTTTCGAAGCTTGACTTTAGAAGGTCTACATTACCTTCAGCAAAGGATTAAGGAGGTGATAGAGATGCAAGCATTGGTTCATCAGTGTAATGCAACAGTGGACTTCATGCTTGAGAAAATGAGGCCGTATCCGCCTACTGTAGTTGATGAAGCACTATATAAACACATCAAGAGCGTTAGTGAAGCTATGGTTGGGGAACCAAATGTGTATCTTCTTCCAATGACTATGGGAGCAGAGGACTTTAGCTTCTACACACATAAAATGTCAGCCGCAATGTTCATGATCGGGACTAAAAACGATACCTTGGGATCGAATGCTACAAGTTTACATTCACCTTACTTGGTCATTGATGAGGAGGTCCTTCCAATAGGAGCAGCTGTTCATGCTGCAGTTGCGATCTCATTCTTGGATACTGATGCTGTTTCTGCCAGTCAGTCATTCACTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

441

Amino Acids

48.03

Weight (kDa)

5.44

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 108 - 423 1.9e-43 Peptidase family M20/M25/M40
M20_dimer PF07687 217 - 313 2.2e-13 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
AccBSI CCGCTC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 898
AccII CGCG 1 cut(s) 316
AciI CCGC 6 cut(s) 314, 316, 446, 834, 1004, 1143
AclWI GGATC 3 cut(s) 125, 138, 1189
AcoI YGGCCR 4 cut(s) 263, 412, 596, 826
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 3 cut(s) 443, 500, 893
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 4 cut(s) 355, 445, 678, 790
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 3 cut(s) 65, 506, 572
AjnI CCWGG 3 cut(s) 391, 631, 794
AjuI GAANNNNNNNTTGG 4 cut(s) 564, 596, 1047, 1079
AloI GAACNNNNNNTCC 2 cut(s) 1236, 1268
Alw21I GWGCWC 4 cut(s) 192, 237, 378, 559
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 3 cut(s) 125, 138, 1189
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 6 cut(s) 263, 412, 596, 792, 826, 995
ApeKI GCWGC 7 cut(s) 10, 310, 695, 698, 731, 1247, 1259
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 1 cut(s) 754
AspS9I GGNCC 2 cut(s) 635, 1231
AsuC2I CCSGG 2 cut(s) 304, 529
AsuHPI GGTGA 3 cut(s) 661, 940, 1197
AsuII TTCGAA 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 2 cut(s) 635, 1231
BalI TGGCCA 3 cut(s) 414, 598, 828
BamHI GGATCC 1 cut(s) 130
BanII GRGCYC 2 cut(s) 192, 237
BauI CACGAG 1 cut(s) 176
Bbv12I GWGCWC 4 cut(s) 192, 237, 378, 559
BbvI GCAGC 6 cut(s) 322, 682, 710, 743, 1246, 1259
BccI CCATC 1 cut(s) 557
BceAI ACGGC 1 cut(s) 982
BcgI CGANNNNNNTGC 2 cut(s) 720, 754
BciT130I CCWGG 3 cut(s) 393, 633, 796
BciVI GTATCC 2 cut(s) 1011, 1276
BcnI CCSGG 2 cut(s) 304, 529
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 2 cut(s) 627, 788
BfmI CTRYAG 4 cut(s) 510, 696, 1011, 1260
BfuAI ACCTGC 1 cut(s) 517
BfuI GTATCC 2 cut(s) 1011, 1276
Bme1390I CCNGG 5 cut(s) 304, 393, 529, 633, 796
Bme18I GGWCC 2 cut(s) 635, 1231
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 2 cut(s) 635, 1231
BmiI GGNNCC 3 cut(s) 132, 301, 1071
BmrFI CCNGG 5 cut(s) 304, 393, 529, 633, 796
BmsI GCATC 4 cut(s) 331, 390, 927, 1279
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 2 cut(s) 87, 186
Bpu14I TTCGAA 1 cut(s) 877
BpuEI CTTGAG 1 cut(s) 1004
BpuMI CCSGG 2 cut(s) 304, 529
BsaJI CCNNGG 9 cut(s) 303, 314, 391, 392, 439, 528, 676, 795, 1176
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 445, 678, 790
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 4 cut(s) 79, 283, 616, 1302
Bse3DI GCAATG 2 cut(s) 747, 1152
BseBI CCWGG 3 cut(s) 393, 633, 796
BseDI CCNNGG 9 cut(s) 303, 314, 391, 392, 439, 528, 676, 795, 1176
BseGI GGATG 2 cut(s) 346, 843
BseLI CCNNNNNNNGG 4 cut(s) 355, 445, 678, 790
BseMI GCAATG 2 cut(s) 747, 1152
BseMII CTCAG 5 cut(s) 78, 83, 92, 177, 340
BseNI ACTGG 4 cut(s) 79, 283, 616, 1302
BseRI GAGGAG 3 cut(s) 215, 530, 1241
BseXI GCAGC 6 cut(s) 322, 682, 710, 743, 1246, 1259
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 316
BshFI GGCC 6 cut(s) 265, 414, 598, 794, 828, 997
BsiHKAI GWGCWC 4 cut(s) 192, 237, 378, 559
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 3 cut(s) 266, 303, 528
BslFI GGGAC 2 cut(s) 883, 1174
BslI CCNNNNNNNGG 4 cut(s) 355, 445, 678, 790
BsmAI GTCTC 1 cut(s) 203
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 883, 1174
BsmI GAATGC 1 cut(s) 1192
BsnI GGCC 6 cut(s) 265, 414, 598, 794, 828, 997
BsoBI CYCGRG 1 cut(s) 771
Bsp119I TTCGAA 1 cut(s) 877
Bsp1286I GDGCHC 4 cut(s) 192, 237, 378, 559
Bsp143I GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
BspACI CCGC 6 cut(s) 314, 316, 446, 834, 1004, 1143
BspANI GGCC 6 cut(s) 265, 414, 598, 794, 828, 997
BspCNI CTCAG 5 cut(s) 79, 84, 93, 178, 339
BspFNI CGCG 1 cut(s) 316
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 3 cut(s) 132, 301, 1071
BspMAI CTGCAG 3 cut(s) 514, 700, 1264
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 3 cut(s) 125, 138, 1189
BspT104I TTCGAA 1 cut(s) 877
BsrBI CCGCTC 1 cut(s) 446
BsrDI GCAATG 2 cut(s) 747, 1152
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 4 cut(s) 79, 283, 616, 1302
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 9 cut(s) 303, 314, 391, 392, 439, 528, 676, 795, 1176
BssMI GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 3 cut(s) 439, 676, 1176
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 3 cut(s) 393, 633, 796
Bst4CI ACNGT 7 cut(s) 490, 673, 808, 824, 856, 970, 1012
Bst6I CTCTTC 1 cut(s) 201
BstBI TTCGAA 1 cut(s) 877
BstC8I GCNNGC 4 cut(s) 192, 405, 736, 942
BstDEI CTNAG 8 cut(s) 87, 92, 101, 186, 273, 326, 450, 1320
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 2 cut(s) 346, 843
BstFNI CGCG 1 cut(s) 316
BstHHI GCGC 1 cut(s) 754
BstKTI GATC 7 cut(s) 133, 230, 744, 765, 1158, 1184, 1272
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
BstMWI GCNNNNNNNGC 8 cut(s) 88, 241, 329, 604, 1003, 1256, 1265, 1298
BstNI CCWGG 3 cut(s) 393, 633, 796
BstNSI RCATGY 2 cut(s) 407, 695
BstSCI CCNGG 5 cut(s) 302, 391, 527, 631, 794
BstSFI CTRYAG 4 cut(s) 510, 696, 1011, 1260
BstUI CGCG 1 cut(s) 316
BstV1I GCAGC 6 cut(s) 322, 682, 710, 743, 1246, 1259
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
BsuI GTATCC 2 cut(s) 1011, 1276
BsuRI GGCC 6 cut(s) 265, 414, 598, 794, 828, 997
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 2 cut(s) 346, 843
BtsIMutI CAGTG 3 cut(s) 861, 962, 975
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 4 cut(s) 192, 405, 736, 942
CciI TCATGA 1 cut(s) 1152
CfoI GCGC 1 cut(s) 754
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 635, 1231
Cfr42I CCGCGG 1 cut(s) 317
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 1 cut(s) 256
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 8 cut(s) 87, 92, 101, 186, 273, 326, 450, 1320
DpnI GATC 7 cut(s) 132, 229, 743, 764, 1157, 1183, 1271
DpnII GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
EaeI YGGCCR 4 cut(s) 263, 412, 596, 826
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 993
Ecl136II GAGCTC 2 cut(s) 190, 235
Eco130I CCWWGG 3 cut(s) 439, 676, 1176
Eco24I GRGCYC 2 cut(s) 192, 237
Eco47I GGWCC 2 cut(s) 635, 1231
Eco53kI GAGCTC 2 cut(s) 190, 235
Eco57I CTGAAG 3 cut(s) 443, 500, 893
Eco88I CYCGRG 1 cut(s) 771
EcoICRI GAGCTC 2 cut(s) 190, 235
EcoO109I RGGNCCY 1 cut(s) 1231
EcoRI GAATTC 1 cut(s) 137
EcoRII CCWGG 3 cut(s) 391, 631, 794
EcoT14I CCWWGG 3 cut(s) 439, 676, 1176
EcoT22I ATGCAT 1 cut(s) 405
EcoT38I GRGCYC 2 cut(s) 192, 237
ErhI CCWWGG 3 cut(s) 439, 676, 1176
Esp3I CGTCTC 1 cut(s) 203
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 2 cut(s) 883, 1174
FauNDI CATATG 2 cut(s) 260, 540
FblI GTMKAC 1 cut(s) 898
FokI GGATG 2 cut(s) 353, 830
FriOI GRGCYC 2 cut(s) 192, 237
FspBI CTAG 2 cut(s) 627, 788
GlaI GCGC 1 cut(s) 753
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 6 cut(s) 265, 414, 598, 794, 828, 997
HapII CCGG 3 cut(s) 266, 303, 528
HhaI GCGC 1 cut(s) 754
Hin6I GCGC 1 cut(s) 752
HinP1I GCGC 1 cut(s) 752
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 880
HinfI GANTC 3 cut(s) 103, 119, 219
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 3 cut(s) 266, 303, 528
HphI GGTGA 3 cut(s) 661, 940, 1197
Hpy166II GTNNAC 6 cut(s) 98, 689, 820, 899, 973, 1199
Hpy188I TCNGA 1 cut(s) 232
Hpy8I GTNNAC 6 cut(s) 98, 689, 820, 899, 973, 1199
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 6 cut(s) 59, 475, 566, 887, 917, 1244
HpyCH4III ACNGT 7 cut(s) 490, 673, 808, 824, 856, 970, 1012
HpyCH4IV ACGT 2 cut(s) 35, 591
HpyCH4V TGCA 9 cut(s) 13, 310, 403, 512, 698, 731, 940, 965, 1262
HpyF10VI GCNNNNNNNGC 8 cut(s) 88, 241, 329, 604, 1003, 1256, 1265, 1298
HpyF3I CTNAG 8 cut(s) 87, 92, 101, 186, 273, 326, 450, 1320
HpySE526I ACGT 2 cut(s) 35, 591
HspAI GCGC 1 cut(s) 752
KspAI GTTAAC 1 cut(s) 820
KspI CCGCGG 1 cut(s) 317
Kzo9I GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
LmnI GCTCC 8 cut(s) 232, 373, 441, 443, 706, 759, 1103, 1244
Lsp1109I GCAGC 6 cut(s) 322, 682, 710, 743, 1246, 1259
LweI GCATC 4 cut(s) 331, 390, 927, 1279
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 2 cut(s) 627, 788
MaeII ACGT 2 cut(s) 35, 591
MaeIII GTNAC 4 cut(s) 427, 453, 667, 802
MalI GATC 7 cut(s) 132, 229, 743, 764, 1157, 1183, 1271
MbiI CCGCTC 1 cut(s) 446
MboI GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
MboII GAAGA 4 cut(s) 218, 409, 1076, 1079
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 4 cut(s) 192, 237, 378, 559
MlsI TGGCCA 3 cut(s) 414, 598, 828
MluCI AATT 5 cut(s) 137, 351, 359, 567, 723
MluNI TGGCCA 3 cut(s) 414, 598, 828
MlyI GAGTC 2 cut(s) 112, 113
MmeI TCCRAC 1 cut(s) 781
Mox20I TGGCCA 3 cut(s) 414, 598, 828
Mph1103I ATGCAT 1 cut(s) 405
MscI TGGCCA 3 cut(s) 414, 598, 828
MseI TTAA 4 cut(s) 545, 819, 864, 921
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 3 cut(s) 414, 598, 828
MspA1I CMGCKG 2 cut(s) 316, 1250
MspI CCGG 3 cut(s) 266, 303, 528
MspR9I CCNGG 5 cut(s) 304, 393, 529, 633, 796
Mva1269I GAATGC 1 cut(s) 1192
MvaI CCWGG 3 cut(s) 393, 633, 796
MvnI CGCG 1 cut(s) 316
MwoI GCNNNNNNNGC 8 cut(s) 88, 241, 329, 604, 1003, 1256, 1265, 1298
NciI CCSGG 2 cut(s) 304, 529
NdeI CATATG 2 cut(s) 260, 540
NdeII GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
NlaIV GGNNCC 3 cut(s) 132, 301, 1071
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 667
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 2 cut(s) 407, 695
NspV TTCGAA 1 cut(s) 877
PaeI GCATGC 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 771
PagI TCATGA 1 cut(s) 1152
PasI CCCWGGG 1 cut(s) 392
PctI GAATGC 1 cut(s) 1192
PfeI GAWTC 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 633
PleI GAGTC 2 cut(s) 111, 113
PpsI GAGTC 2 cut(s) 111, 113
PpuMI RGGWCCY 1 cut(s) 1231
Psp124BI GAGCTC 2 cut(s) 192, 237
Psp5II RGGWCCY 1 cut(s) 1231
Psp6I CCWGG 3 cut(s) 391, 631, 794
PspGI CCWGG 3 cut(s) 391, 631, 794
PspN4I GGNNCC 3 cut(s) 132, 301, 1071
PspPI GGNCC 2 cut(s) 635, 1231
PspPPI RGGWCCY 1 cut(s) 1231
PstI CTGCAG 3 cut(s) 514, 700, 1264
PsuI RGATCY 1 cut(s) 130
PsyI GACNNNGTC 1 cut(s) 633
PvuII CAGCTG 1 cut(s) 1250
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 408
SacI GAGCTC 2 cut(s) 192, 237
SacII CCGCGG 1 cut(s) 317
SaqAI TTAA 4 cut(s) 545, 819, 864, 921
Sau3AI GATC 7 cut(s) 130, 227, 741, 762, 1155, 1181, 1269
Sau96I GGNCC 2 cut(s) 635, 1231
SchI GAGTC 2 cut(s) 112, 113
ScrFI CCNGG 5 cut(s) 304, 393, 529, 633, 796
SduI GDGCHC 4 cut(s) 192, 237, 378, 559
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 4 cut(s) 331, 390, 927, 1279
SfcI CTRYAG 4 cut(s) 510, 696, 1011, 1260
Sfr274I CTCGAG 1 cut(s) 771
Sfr303I CCGCGG 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 877
SgrBI CCGCGG 1 cut(s) 317
SinI GGWCC 2 cut(s) 635, 1231
SlaI CTCGAG 1 cut(s) 771
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 771, 983
SmoI CTYRAG 2 cut(s) 771, 983
SphI GCATGC 1 cut(s) 407
Sse9I AATT 5 cut(s) 137, 351, 359, 567, 723
SsiI CCGC 6 cut(s) 314, 316, 446, 834, 1004, 1143
SspI AATATT 1 cut(s) 579
SspMI CTAG 2 cut(s) 627, 788
SstI GAGCTC 2 cut(s) 192, 237
StyD4I CCNGG 5 cut(s) 302, 391, 527, 631, 794
StyI CCWWGG 3 cut(s) 439, 676, 1176
TaaI ACNGT 7 cut(s) 490, 673, 808, 824, 856, 970, 1012
TaiI ACGT 2 cut(s) 38, 594
TaqI TCGA 6 cut(s) 32, 237, 740, 772, 877, 1184
TasI AATT 5 cut(s) 137, 351, 359, 567, 723
TatI WGTACW 1 cut(s) 255
TauI GCSGC 4 cut(s) 316, 449, 836, 1145
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 4 cut(s) 545, 819, 864, 921
Tru9I TTAA 4 cut(s) 545, 819, 864, 921
TscAI CASTG 3 cut(s) 861, 962, 975
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 7 cut(s) 10, 310, 695, 698, 731, 1247, 1259
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 8 cut(s) 35, 211, 413, 941, 967, 1035, 1141, 1244
TspRI CASTG 3 cut(s) 861, 962, 975
Tth111I GACNNNGTC 1 cut(s) 633
VpaK11BI GGWCC 2 cut(s) 635, 1231
XapI RAATTY 1 cut(s) 137
XbaI TCTAGA 2 cut(s) 626, 787
XceI RCATGY 2 cut(s) 407, 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 683
XhoI CTCGAG 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 898
XspI CTAG 2 cut(s) 627, 788
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.