MD12G1032400.v1.1

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015657
Physical Location & Seq
Reverse (-)
3566482 .. 3569888
3407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1032400.v1.1.491

Sequence Viewer

Length: 1308 bp
ATGGAGTGGCTGCGGCTGTTGATATTGTTATTTACGTTCACAAACCACGTATCGTCATGGGGTTCAGTGGCGGCCGGTTCGAGGTGGGAGGTGAGTCAACTCACTCGAGAGCTGATGGACTCGGCGCGGGAGCCCGAGTTCTTTGATTGGCTGAAGAGGGTTAGGAGGACAATCCACGAGAACCCGGAGCTGGCCTTTGAGGAGGAGGAGACGAGTCGACTCATCCGATCGGAACTCGACTCGCTCGAGATCGGGTACACGTGGCCGGTGGCGAAGACCGGGGTGGTGGCTTCTATTGGTTCGGGGTCGCAGCCATGGTTTGCTCTCAGGGCCGATATGGATGCTCTCCCGATTCAGGAAATGGTTGAGTGGGAGCATAAGAGCAAGAACCCTGGAAAAATGCATGCTTGTGGGCATGATGCTCATGCAACTATGCTGCTTGGAGCTGCTAAGTTACTTCAACGCAAAAGCAAAGAAATAAAGGGCACAATCAAGCTTGTTTTTCAACCTGCAGAAGAGGGCCGTGGAGGGGCTTACCATATGATAAAAGAGGGTGCCCTTGACAACATTCAAGGCATTCTTGGGTTGCATATTTCACCGGAAATGCCTGTCGGAACTATTGGTTCGAGACCTGGTCCGATGCTTGCTGCTGCTGGTAGGTTTTTGGTCACCATTCATGGGAAAGGTGGGCATGCTGCATCTCCACATTTGGCCACAGATCCGATTCTTGCTGCCTGCTTGACCATCATTTCCCTCCAACAGATTGTATCTCGAGAAACAAACCCTCTAGAGTCCAGGGTCGTAACTGTGGGGTTTGTTGATGGTGGCCAAGCAGGAAATGTAATCCCAGAGACGGTGACATTAAAAGGCACGTTTCGAAGCATGACTTCCGAAGGTCTATACTACCTTCAGCAAAGGATTAAAGAGGTTACAGAGATGCAAGCTTCGGTGCATAGGTGCACAGCAACGGTAGACTTCATGCTGGAAAAAATGAGGCCCTATCCTGCCACGGTTAATGATGAAGCAATGTACAAACATGCAAAGAGTGTTGGAGAAACTTTGCTCGGGGAACCAAATGTGAAGCTACTTCCAATGGGTATGGGAGCAGAGGACTTTAGCTTCTATGCAGAAAAAATGGCTGCTGCATTCTTCATGATTGGGACGAAGAACGCGACGTTTGTATCGAAAACAGATTTGCATTCACCCTTCCTGGTGATTGATGAGGAGGTTCTTCCCATTGGAGCAGCCTTTCATGCTGCAGTTGCACTCTCATATTTGGATAATGTTGATGCTGTTGTGACGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

436

Amino Acids

47.79

Weight (kDa)

5.91

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 108 - 422 3.4e-44 Peptidase family M20/M25/M40
M20_dimer PF07687 220 - 310 6.1e-10 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 554
AccI GTMKAC 2 cut(s) 217, 972
AccII CGCG 2 cut(s) 127, 1172
AciI CCGC 3 cut(s) 13, 71, 127
AclWI GGATC 1 cut(s) 713
AcoI YGGCCR 4 cut(s) 72, 263, 711, 826
AcuI CTGAAG 2 cut(s) 173, 893
AcvI CACGTG 1 cut(s) 261
AfaI GTAC 2 cut(s) 257, 1031
AfiI CCNNNNNNNGG 6 cut(s) 81, 190, 355, 529, 678, 853
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 3 cut(s) 461, 506, 572
AjnI CCWGG 4 cut(s) 391, 631, 794, 1209
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 7 cut(s) 112, 190, 446, 496, 944, 1084, 1119
AluI AGCT 7 cut(s) 112, 190, 446, 496, 944, 1084, 1119
Alw21I GWGCWC 1 cut(s) 962
Alw26I GTCTC 3 cut(s) 203, 622, 845
Alw44I GTGCAC 1 cut(s) 958
AlwI GGATC 1 cut(s) 713
Ama87I CYCGRG 5 cut(s) 105, 134, 245, 771, 1064
AoxI GGCC 8 cut(s) 72, 192, 263, 330, 520, 711, 826, 995
ApaLI GTGCAC 1 cut(s) 958
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 4 cut(s) 330, 520, 635, 996
AsuC2I CCSGG 2 cut(s) 185, 280
AsuHPI GGTGA 6 cut(s) 103, 588, 661, 868, 1194, 1225
AsuII TTCGAA 1 cut(s) 877
AvaI CYCGRG 5 cut(s) 105, 134, 245, 771, 1064
AvaII GGWCC 1 cut(s) 635
BaeGI GKGCMC 3 cut(s) 488, 559, 962
BaeI ACNNNNGTAYC 2 cut(s) 1164, 1197
BalI TGGCCA 2 cut(s) 713, 828
BanI GGYRCC 1 cut(s) 554
BanII GRGCYC 1 cut(s) 135
BauI CACGAG 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 962
BccI CCATC 3 cut(s) 109, 752, 815
BceAI ACGGC 1 cut(s) 507
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 4 cut(s) 393, 633, 796, 1211
BcnI CCSGG 2 cut(s) 185, 280
BcoDI GTCTC 3 cut(s) 203, 622, 845
BfaI CTAG 1 cut(s) 788
BfmI CTRYAG 2 cut(s) 510, 1257
BfuAI ACCTGC 1 cut(s) 517
Bme1390I CCNGG 6 cut(s) 185, 280, 393, 633, 796, 1211
Bme18I GGWCC 1 cut(s) 635
BmeT110I CYCGRG 5 cut(s) 105, 134, 245, 771, 1064
BmgT120I GGNCC 4 cut(s) 330, 520, 635, 996
BmiI GGNNCC 3 cut(s) 132, 556, 1071
BmrFI CCNGG 6 cut(s) 185, 280, 393, 633, 796, 1211
BmsI GCATC 6 cut(s) 331, 409, 630, 707, 927, 1279
BpiI GAAGAC 1 cut(s) 281
Bpu14I TTCGAA 1 cut(s) 877
BpuMI CCSGG 2 cut(s) 185, 280
BsaAI YACGTR 2 cut(s) 49, 261
BsaI GGTCTC 1 cut(s) 622
BsaJI CCNNGG 6 cut(s) 279, 314, 391, 523, 795, 1008
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 6 cut(s) 81, 190, 355, 529, 678, 853
Bse118I RCCGGY 2 cut(s) 74, 265
Bse3DI GCAATG 1 cut(s) 1032
BseBI CCWGG 4 cut(s) 393, 633, 796, 1211
BseDI CCNNGG 6 cut(s) 279, 314, 391, 523, 795, 1008
BseGI GGATG 2 cut(s) 222, 346
BseLI CCNNNNNNNGG 6 cut(s) 81, 190, 355, 529, 678, 853
BseMI GCAATG 1 cut(s) 1032
BseMII CTCAG 1 cut(s) 340
BseRI GAGGAG 4 cut(s) 215, 218, 221, 1238
BseSI GKGCMC 3 cut(s) 488, 559, 962
BseX3I CGGCCG 1 cut(s) 72
Bsh1236I CGCG 2 cut(s) 127, 1172
Bsh1285I CGRYCG 2 cut(s) 75, 230
BshFI GGCC 8 cut(s) 74, 194, 265, 332, 522, 713, 828, 997
BshNI GGYRCC 1 cut(s) 554
BsiEI CGRYCG 2 cut(s) 75, 230
BsiHKAI GWGCWC 1 cut(s) 962
BsiHKCI CYCGRG 5 cut(s) 105, 134, 245, 771, 1064
BsiSI CCGG 5 cut(s) 75, 185, 266, 279, 599
BslFI GGGAC 1 cut(s) 1174
BslI CCNNNNNNNGG 6 cut(s) 81, 190, 355, 529, 678, 853
BsmAI GTCTC 3 cut(s) 203, 622, 845
BsmBI CGTCTC 2 cut(s) 203, 845
BsmFI GGGAC 1 cut(s) 1174
BsmI GAATGC 3 cut(s) 576, 1145, 1198
BsnI GGCC 8 cut(s) 74, 194, 265, 332, 522, 713, 828, 997
Bso31I GGTCTC 1 cut(s) 622
BsoBI CYCGRG 5 cut(s) 105, 134, 245, 771, 1064
Bsp119I TTCGAA 1 cut(s) 877
Bsp1286I GDGCHC 4 cut(s) 135, 488, 559, 962
Bsp1407I TGTACA 1 cut(s) 1029
Bsp143I GATC 3 cut(s) 227, 249, 718
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 3 cut(s) 13, 71, 127
BspANI GGCC 8 cut(s) 74, 194, 265, 332, 522, 713, 828, 997
BspCNI CTCAG 1 cut(s) 339
BspFNI CGCG 2 cut(s) 127, 1172
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 3 cut(s) 132, 556, 1071
BspMAI CTGCAG 2 cut(s) 514, 1261
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 1 cut(s) 713
BspT104I TTCGAA 1 cut(s) 877
BspT107I GGYRCC 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 622
BsrDI GCAATG 1 cut(s) 1032
BsrFI RCCGGY 2 cut(s) 74, 265
BsrGI TGTACA 1 cut(s) 1029
BssAI RCCGGY 2 cut(s) 74, 265
BssECI CCNNGG 6 cut(s) 279, 314, 391, 523, 795, 1008
BssMI GATC 3 cut(s) 227, 249, 718
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 314
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 4 cut(s) 393, 633, 796, 1211
Bst4CI ACNGT 4 cut(s) 808, 856, 970, 1012
Bst6I CTCTTC 2 cut(s) 149, 510
BstAUI TGTACA 1 cut(s) 1029
BstBAI YACGTR 2 cut(s) 49, 261
BstBI TTCGAA 1 cut(s) 877
BstC8I GCNNGC 6 cut(s) 192, 405, 645, 693, 736, 942
BstDEI CTNAG 2 cut(s) 326, 450
BstDSI CCRYGG 3 cut(s) 314, 523, 1008
BstEII GGTNACC 1 cut(s) 667
BstF5I GGATG 2 cut(s) 222, 346
BstFNI CGCG 2 cut(s) 127, 1172
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 3 cut(s) 230, 252, 721
BstMAI GTCTC 3 cut(s) 203, 622, 845
BstMBI GATC 3 cut(s) 227, 249, 718
BstMCI CGRYCG 2 cut(s) 75, 230
BstMWI GCNNNNNNNGC 3 cut(s) 329, 1253, 1262
BstNI CCWGG 4 cut(s) 393, 633, 796, 1211
BstNSI RCATGY 3 cut(s) 407, 695, 1040
BstPI GGTNACC 1 cut(s) 667
BstSCI CCNGG 6 cut(s) 183, 278, 391, 631, 794, 1209
BstSFI CTRYAG 2 cut(s) 510, 1257
BstSLI GKGCMC 3 cut(s) 488, 559, 962
BstUI CGCG 2 cut(s) 127, 1172
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 72
BsuRI GGCC 8 cut(s) 74, 194, 265, 332, 522, 713, 828, 997
BtgI CCRYGG 3 cut(s) 314, 523, 1008
BtsCI GGATG 2 cut(s) 222, 346
BtsIMutI CAGTG 1 cut(s) 72
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 6 cut(s) 192, 405, 645, 693, 736, 942
CciI TCATGA 1 cut(s) 1152
CfoI GCGC 1 cut(s) 127
Cfr10I RCCGGY 2 cut(s) 74, 265
Cfr13I GGNCC 4 cut(s) 330, 520, 635, 996
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 2 cut(s) 256, 1030
CviQI GTAC 2 cut(s) 256, 1030
DdeI CTNAG 2 cut(s) 326, 450
DpnI GATC 3 cut(s) 229, 251, 720
DpnII GATC 3 cut(s) 227, 249, 718
EaeI YGGCCR 4 cut(s) 72, 263, 711, 826
EagI CGGCCG 1 cut(s) 72
Eam1104I CTCTTC 2 cut(s) 149, 510
EarI CTCTTC 2 cut(s) 149, 510
EclXI CGGCCG 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 635
Eco52I CGGCCG 1 cut(s) 72
Eco57I CTGAAG 2 cut(s) 173, 893
Eco72I CACGTG 1 cut(s) 261
Eco88I CYCGRG 5 cut(s) 105, 134, 245, 771, 1064
Eco91I GGTNACC 1 cut(s) 667
EcoO109I RGGNCCY 1 cut(s) 996
EcoO65I GGTNACC 1 cut(s) 667
EcoRII CCWGG 4 cut(s) 391, 631, 794, 1209
EcoT14I CCWWGG 1 cut(s) 314
EcoT22I ATGCAT 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 314
Esp3I CGTCTC 2 cut(s) 203, 845
FalI AAGNNNNNCTT 4 cut(s) 564, 596, 871, 903
FaqI GGGAC 1 cut(s) 1174
FauI CCCGC 1 cut(s) 120
FauNDI CATATG 1 cut(s) 540
FblI GTMKAC 2 cut(s) 217, 972
FokI GGATG 2 cut(s) 209, 353
FriOI GRGCYC 1 cut(s) 135
FspBI CTAG 1 cut(s) 788
GlaI GCGC 1 cut(s) 126
HaeIII GGCC 8 cut(s) 74, 194, 265, 332, 522, 713, 828, 997
HapII CCGG 5 cut(s) 75, 185, 266, 279, 599
HhaI GCGC 1 cut(s) 127
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 2 cut(s) 98, 218
HindII GTYRAC 2 cut(s) 98, 218
HindIII AAGCTT 2 cut(s) 494, 942
HinfI GANTC 8 cut(s) 94, 119, 214, 219, 239, 352, 724, 791
HpaII CCGG 5 cut(s) 75, 185, 266, 279, 599
HphI GGTGA 6 cut(s) 103, 588, 661, 868, 1194, 1225
Hpy166II GTNNAC 6 cut(s) 39, 98, 218, 258, 960, 973
Hpy188I TCNGA 6 cut(s) 227, 232, 614, 639, 723, 892
Hpy188III TCNNGA 9 cut(s) 107, 247, 349, 356, 627, 771, 773, 788, 1153
Hpy8I GTNNAC 6 cut(s) 39, 98, 218, 258, 960, 973
Hpy99I CGWCG 1 cut(s) 1177
HpyAV CCTTC 3 cut(s) 887, 917, 1216
HpyCH4III ACNGT 4 cut(s) 808, 856, 970, 1012
HpyCH4IV ACGT 5 cut(s) 35, 48, 260, 872, 1175
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 1253, 1262
HpyF3I CTNAG 2 cut(s) 326, 450
HpySE526I ACGT 5 cut(s) 35, 48, 260, 872, 1175
HspAI GCGC 1 cut(s) 125
Kzo9I GATC 3 cut(s) 227, 249, 718
LmnI GCTCC 6 cut(s) 130, 187, 373, 443, 1103, 1241
LweI GCATC 6 cut(s) 331, 409, 630, 707, 927, 1279
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 1 cut(s) 788
MaeII ACGT 5 cut(s) 35, 48, 260, 872, 1175
MaeIII GTNAC 6 cut(s) 453, 667, 802, 856, 928, 1297
MalI GATC 3 cut(s) 229, 251, 720
MboI GATC 3 cut(s) 227, 249, 718
MboII GAAGA 6 cut(s) 166, 286, 527, 1141, 1177, 1223
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 4 cut(s) 135, 488, 559, 962
MlsI TGGCCA 2 cut(s) 713, 828
MluNI TGGCCA 2 cut(s) 713, 828
MlyI GAGTC 6 cut(s) 103, 113, 213, 223, 233, 800
MmeI TCCRAC 3 cut(s) 592, 781, 1030
Mox20I TGGCCA 2 cut(s) 713, 828
Mph1103I ATGCAT 1 cut(s) 405
MscI TGGCCA 2 cut(s) 713, 828
MseI TTAA 3 cut(s) 863, 921, 1014
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 2 cut(s) 713, 828
MspI CCGG 5 cut(s) 75, 185, 266, 279, 599
MspR9I CCNGG 6 cut(s) 185, 280, 393, 633, 796, 1211
Mva1269I GAATGC 3 cut(s) 576, 1145, 1198
MvaI CCWGG 4 cut(s) 393, 633, 796, 1211
MvnI CGCG 2 cut(s) 127, 1172
MwoI GCNNNNNNNGC 3 cut(s) 329, 1253, 1262
NciI CCSGG 2 cut(s) 185, 280
NcoI CCATGG 1 cut(s) 314
NdeI CATATG 1 cut(s) 540
NdeII GATC 3 cut(s) 227, 249, 718
NlaIV GGNNCC 3 cut(s) 132, 556, 1071
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 3 cut(s) 667, 856, 1297
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 3 cut(s) 407, 695, 1040
NspV TTCGAA 1 cut(s) 877
PaeI GCATGC 2 cut(s) 407, 695
PaeR7I CTCGAG 3 cut(s) 105, 245, 771
PagI TCATGA 1 cut(s) 1152
PcsI WCGNNNNNNNCGW 4 cut(s) 223, 243, 1169, 1181
PctI GAATGC 3 cut(s) 576, 1145, 1198
PfeI GAWTC 2 cut(s) 352, 724
PflFI GACNNNGTC 1 cut(s) 633
Ple19I CGATCG 1 cut(s) 230
PleI GAGTC 6 cut(s) 102, 113, 213, 222, 233, 799
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 6 cut(s) 102, 113, 213, 222, 233, 799
Ppu21I YACGTR 2 cut(s) 49, 261
Psp6I CCWGG 4 cut(s) 391, 631, 794, 1209
PspCI CACGTG 1 cut(s) 261
PspEI GGTNACC 1 cut(s) 667
PspGI CCWGG 4 cut(s) 391, 631, 794, 1209
PspN4I GGNNCC 3 cut(s) 132, 556, 1071
PspPI GGNCC 4 cut(s) 330, 520, 635, 996
PstI CTGCAG 2 cut(s) 514, 1261
PsuI RGATCY 1 cut(s) 718
PsyI GACNNNGTC 1 cut(s) 633
PvuI CGATCG 1 cut(s) 230
RsaI GTAC 2 cut(s) 257, 1031
RsaNI GTAC 2 cut(s) 256, 1030
RseI CAYNNNNRTG 1 cut(s) 408
SalI GTCGAC 1 cut(s) 216
SaqAI TTAA 3 cut(s) 863, 921, 1014
Sau3AI GATC 3 cut(s) 227, 249, 718
Sau96I GGNCC 4 cut(s) 330, 520, 635, 996
SchI GAGTC 6 cut(s) 103, 113, 213, 223, 233, 800
ScrFI CCNGG 6 cut(s) 185, 280, 393, 633, 796, 1211
SduI GDGCHC 4 cut(s) 135, 488, 559, 962
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 6 cut(s) 331, 409, 630, 707, 927, 1279
SfcI CTRYAG 2 cut(s) 510, 1257
Sfr274I CTCGAG 3 cut(s) 105, 245, 771
SfuI TTCGAA 1 cut(s) 877
SinI GGWCC 1 cut(s) 635
SlaI CTCGAG 3 cut(s) 105, 245, 771
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 3 cut(s) 105, 245, 771
SmoI CTYRAG 3 cut(s) 105, 245, 771
SphI GCATGC 2 cut(s) 407, 695
SsiI CCGC 3 cut(s) 13, 71, 127
SspMI CTAG 1 cut(s) 788
StyD4I CCNGG 6 cut(s) 183, 278, 391, 631, 794, 1209
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 4 cut(s) 808, 856, 970, 1012
TaiI ACGT 5 cut(s) 38, 51, 263, 875, 1178
TaqI TCGA 9 cut(s) 80, 106, 217, 237, 246, 626, 772, 877, 1184
TatI WGTACW 1 cut(s) 1029
TauI GCSGC 2 cut(s) 16, 74
TfiI GAWTC 2 cut(s) 352, 724
Tru1I TTAA 3 cut(s) 863, 921, 1014
Tru9I TTAA 3 cut(s) 863, 921, 1014
TscAI CASTG 1 cut(s) 72
TseFI GTSAC 3 cut(s) 667, 856, 1297
Tsp45I GTSAC 3 cut(s) 667, 856, 1297
TspDTI ATGAA 5 cut(s) 665, 967, 1035, 1141, 1241
TspRI CASTG 1 cut(s) 72
Tth111I GACNNNGTC 1 cut(s) 633
VneI GTGCAC 1 cut(s) 958
VpaK11BI GGWCC 1 cut(s) 635
XbaI TCTAGA 1 cut(s) 787
XceI RCATGY 3 cut(s) 407, 695, 1040
XhoI CTCGAG 3 cut(s) 105, 245, 771
XmiI GTMKAC 2 cut(s) 217, 972
XspI CTAG 1 cut(s) 788
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.