Prupe.7G099700_v2.0.a1

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0015657
Physical Location & Seq
Forward (+)
13036352 .. 13039392
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G099700.1

Sequence Viewer

Length: 894 bp
ATGGGAGCACAAGAGCAGGAACCCTGGAAAGATGCTCATGTAACAATGCTGCTTGGAGCAGCTAAGTTACTTCAGAGCAAAAGCAAAGAAATAAAGGGCACAATCAAGCTTGTTTTTCAACCTGGAGAAGAGGAACATGCAGGGGCTTACCATATGATAAAAGAGGGTGCTCTTGATAAGATTCAAGGCATTTTTGGGTTACATATTCAACCTCTAATGCCCGTTGGTACTATTGGTTCCAGGCCAGGTCCAATTCTTGCAGGCTCTGGTAGATTTTCAGTCACAATTCATGGAAAAGGTGGGCATGCTGCGTTTCCCCATTTGGCCACAGATCCAATTCTTGCTGCCTGCTCGGCCATCCTTGCCCTTCAACAGCTTATTTCTAGAGAAACAGACCCTCTAGATGCCAGGGTGGTTACTGTTGGGATGATTGAGGGTGGCCAAGCAGCAAATATAATCCCAGAGACAGTGAGACTTAAGGGCACTTTTCGAAGCATGACTTCAGAAGGTCTAAACTACCTTCAGCAAAGGATTCAAGAGGTCATAGAGATACAGGCTTCGGTGCACGGGTGCACAGCAACGGTAGACTTCATGCTGGAAAAAATGAAGCCCTATCCAGCCACAGTTAATGATGAAGCAATGTACAAACATGCAAAGAATGTGGGAGAAACTTTGCTTGGTGAACCAAATGTGAAGCTTTTTCCAATGTCTATGGGAGCAGAGGACTTCAGCTTTTACACAGAAAAAATGGCAGCTGCATTCTTCATGATTGGGACAAAGAACGAGACTTTGGACCCAAATACAATTTGGCATTCACCCAATTTGGTAATTGATGAGGAGGTTCTTCCAATAGGAGCTGCCCTTCATGCTGCTGTTGCAATTTCTTACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

298

Amino Acids

32.1

Weight (kDa)

5.76

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 892
AccI GTMKAC 1 cut(s) 585
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 3 cut(s) 324, 354, 439
AcuI CTGAAG 4 cut(s) 56, 486, 506, 712
AfaI GTAC 2 cut(s) 229, 644
AflII CTTAAG 1 cut(s) 476
AgsI TTSAA 5 cut(s) 119, 185, 209, 371, 536
AjnI CCWGG 5 cut(s) 23, 121, 239, 244, 407
AjuI GAANNNNNNNTTGG 4 cut(s) 660, 692, 773, 805
AluBI AGCT 7 cut(s) 62, 109, 376, 697, 732, 755, 857
AluI AGCT 7 cut(s) 62, 109, 376, 697, 732, 755, 857
Alw21I GWGCWC 4 cut(s) 10, 172, 567, 575
Alw26I GTCTC 3 cut(s) 458, 466, 779
Alw44I GTGCAC 2 cut(s) 563, 571
AlwI GGATC 1 cut(s) 326
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 4 cut(s) 242, 324, 354, 439
ApaLI GTGCAC 2 cut(s) 563, 571
ApeKI GCWGC 9 cut(s) 49, 59, 308, 344, 446, 752, 755, 857, 869
AspS9I GGNCC 2 cut(s) 248, 793
AsuHPI GGTGA 2 cut(s) 692, 807
AsuII TTCGAA 1 cut(s) 490
AvaII GGWCC 2 cut(s) 248, 793
BaeGI GKGCMC 4 cut(s) 101, 485, 567, 575
BalI TGGCCA 2 cut(s) 326, 441
Bbv12I GWGCWC 4 cut(s) 10, 172, 567, 575
BbvI GCAGC 9 cut(s) 36, 71, 295, 331, 458, 742, 764, 844, 856
BccI CCATC 1 cut(s) 365
BciT130I CCWGG 5 cut(s) 25, 123, 241, 246, 409
BcoDI GTCTC 3 cut(s) 458, 466, 779
BfaI CTAG 2 cut(s) 384, 401
BfrI CTTAAG 1 cut(s) 476
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 9 cut(s) 50, 60, 309, 345, 447, 753, 756, 858, 870
BlsI GCNGC 9 cut(s) 51, 61, 310, 346, 448, 754, 757, 859, 871
Bme1390I CCNGG 5 cut(s) 25, 123, 241, 246, 409
Bme18I GGWCC 2 cut(s) 248, 793
BmgT120I GGNCC 2 cut(s) 248, 793
BmiI GGNNCC 3 cut(s) 21, 238, 795
BmrFI CCNGG 5 cut(s) 25, 123, 241, 246, 409
BmsI GCATC 2 cut(s) 22, 394
BpmI CTGGAG 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 23, 408
Bse3DI GCAATG 1 cut(s) 645
BseBI CCWGG 5 cut(s) 25, 123, 241, 246, 409
BseDI CCNNGG 2 cut(s) 23, 408
BseGI GGATG 2 cut(s) 357, 432
BseMI GCAATG 1 cut(s) 645
BseRI GAGGAG 1 cut(s) 851
BseSI GKGCMC 4 cut(s) 101, 485, 567, 575
BseXI GCAGC 9 cut(s) 36, 71, 295, 331, 458, 742, 764, 844, 856
BshFI GGCC 4 cut(s) 244, 326, 356, 441
BsiHKAI GWGCWC 4 cut(s) 10, 172, 567, 575
BslFI GGGAC 1 cut(s) 787
BsmAI GTCTC 3 cut(s) 458, 466, 779
BsmFI GGGAC 1 cut(s) 787
BsmI GAATGC 2 cut(s) 758, 811
BsnI GGCC 4 cut(s) 244, 326, 356, 441
Bsp119I TTCGAA 1 cut(s) 490
Bsp1286I GDGCHC 6 cut(s) 10, 101, 172, 485, 567, 575
Bsp1407I TGTACA 1 cut(s) 642
Bsp143I GATC 1 cut(s) 331
BspANI GGCC 4 cut(s) 244, 326, 356, 441
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 3 cut(s) 21, 238, 795
BspPI GGATC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 490
BspTI CTTAAG 1 cut(s) 476
BsrDI GCAATG 1 cut(s) 645
BsrGI TGTACA 1 cut(s) 642
BssECI CCNNGG 2 cut(s) 23, 408
BssMI GATC 1 cut(s) 331
Bst2UI CCWGG 5 cut(s) 25, 123, 241, 246, 409
Bst4CI ACNGT 4 cut(s) 421, 469, 583, 625
Bst6I CTCTTC 1 cut(s) 123
BstAFI CTTAAG 1 cut(s) 476
BstAUI TGTACA 1 cut(s) 642
BstBI TTCGAA 1 cut(s) 490
BstC8I GCNNGC 3 cut(s) 262, 306, 349
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 2 cut(s) 357, 432
BstKTI GATC 1 cut(s) 334
BstMAI GTCTC 3 cut(s) 458, 466, 779
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 4 cut(s) 353, 362, 866, 875
BstNI CCWGG 5 cut(s) 25, 123, 241, 246, 409
BstNSI RCATGY 3 cut(s) 140, 308, 653
BstSCI CCNGG 5 cut(s) 23, 121, 239, 244, 407
BstSLI GKGCMC 4 cut(s) 101, 485, 567, 575
BstV1I GCAGC 9 cut(s) 36, 71, 295, 331, 458, 742, 764, 844, 856
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 4 cut(s) 244, 326, 356, 441
BtsCI GGATG 2 cut(s) 357, 432
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 3 cut(s) 262, 306, 349
CaiI CAGNNNCTG 1 cut(s) 266
CciI TCATGA 1 cut(s) 765
Cfr13I GGNCC 2 cut(s) 248, 793
Csp6I GTAC 2 cut(s) 228, 643
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 9 cut(s) 38, 137, 290, 305, 496, 592, 650, 766, 866
CviQI GTAC 2 cut(s) 228, 643
DdeI CTNAG 1 cut(s) 63
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
EaeI YGGCCR 3 cut(s) 324, 354, 439
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 248, 793
Eco57I CTGAAG 4 cut(s) 56, 486, 506, 712
EcoRII CCWGG 5 cut(s) 23, 121, 239, 244, 407
FaeI CATG 9 cut(s) 41, 140, 293, 308, 499, 595, 653, 769, 869
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FaqI GGGAC 1 cut(s) 787
FatI CATG 9 cut(s) 37, 136, 289, 304, 495, 591, 649, 765, 865
FauNDI CATATG 1 cut(s) 153
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 9 cut(s) 50, 60, 309, 345, 447, 753, 756, 858, 870
FokI GGATG 2 cut(s) 344, 439
Fsp4HI GCNGC 9 cut(s) 50, 60, 309, 345, 447, 753, 756, 858, 870
FspBI CTAG 2 cut(s) 384, 401
GluI GCNGC 9 cut(s) 50, 60, 309, 345, 447, 753, 756, 858, 870
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 4 cut(s) 244, 326, 356, 441
Hin1II CATG 9 cut(s) 41, 140, 293, 308, 499, 595, 653, 769, 869
HindIII AAGCTT 2 cut(s) 107, 695
HinfI GANTC 2 cut(s) 181, 532
HphI GGTGA 2 cut(s) 692, 807
Hpy166II GTNNAC 4 cut(s) 565, 573, 586, 683
Hpy188I TCNGA 2 cut(s) 75, 505
Hpy188III TCNNGA 5 cut(s) 173, 384, 401, 536, 766
Hpy8I GTNNAC 4 cut(s) 565, 573, 586, 683
HpyAV CCTTC 4 cut(s) 377, 500, 530, 872
HpyCH4III ACNGT 4 cut(s) 421, 469, 583, 625
HpyCH4V TGCA 7 cut(s) 140, 260, 565, 573, 653, 758, 878
HpyF10VI GCNNNNNNNGC 4 cut(s) 353, 362, 866, 875
HpyF3I CTNAG 1 cut(s) 63
Hsp92II CATG 9 cut(s) 41, 140, 293, 308, 499, 595, 653, 769, 869
Kzo9I GATC 1 cut(s) 331
LmnI GCTCC 4 cut(s) 5, 56, 716, 854
Lsp1109I GCAGC 9 cut(s) 36, 71, 295, 331, 458, 742, 764, 844, 856
LweI GCATC 2 cut(s) 22, 394
MaeI CTAG 2 cut(s) 384, 401
MaeIII GTNAC 5 cut(s) 40, 66, 198, 280, 415
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 3 cut(s) 140, 754, 836
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 6 cut(s) 10, 101, 172, 485, 567, 575
MlsI TGGCCA 2 cut(s) 326, 441
MluCI AATT 7 cut(s) 252, 285, 336, 804, 820, 828, 879
MluNI TGGCCA 2 cut(s) 326, 441
MnlI CCTC 9 cut(s) 124, 157, 222, 408, 427, 532, 715, 829, 832
Mox20I TGGCCA 2 cut(s) 326, 441
MscI TGGCCA 2 cut(s) 326, 441
MseI TTAA 2 cut(s) 477, 627
Msp20I TGGCCA 2 cut(s) 326, 441
MspA1I CMGCKG 1 cut(s) 755
MspCI CTTAAG 1 cut(s) 476
MspR9I CCNGG 5 cut(s) 25, 123, 241, 246, 409
Mva1269I GAATGC 2 cut(s) 758, 811
MvaI CCWGG 5 cut(s) 25, 123, 241, 246, 409
MwoI GCNNNNNNNGC 4 cut(s) 353, 362, 866, 875
NdeI CATATG 1 cut(s) 153
NdeII GATC 1 cut(s) 331
NlaIII CATG 9 cut(s) 41, 140, 293, 308, 499, 595, 653, 769, 869
NlaIV GGNNCC 3 cut(s) 21, 238, 795
NmeAIII GCCGAG 1 cut(s) 332
NmuCI GTSAC 1 cut(s) 280
NspI RCATGY 3 cut(s) 140, 308, 653
NspV TTCGAA 1 cut(s) 490
PaeI GCATGC 1 cut(s) 308
PagI TCATGA 1 cut(s) 765
PctI GAATGC 2 cut(s) 758, 811
PfeI GAWTC 2 cut(s) 181, 532
PkrI GCNGC 9 cut(s) 51, 61, 310, 346, 448, 754, 757, 859, 871
PsiI TTATAA 1 cut(s) 892
Psp6I CCWGG 5 cut(s) 23, 121, 239, 244, 407
PspGI CCWGG 5 cut(s) 23, 121, 239, 244, 407
PspN4I GGNNCC 3 cut(s) 21, 238, 795
PspPI GGNCC 2 cut(s) 248, 793
PsrI GAACNNNNNNTAC 2 cut(s) 220, 252
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 331
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 2 cut(s) 229, 644
RsaNI GTAC 2 cut(s) 228, 643
SaqAI TTAA 2 cut(s) 477, 627
SatI GCNGC 9 cut(s) 50, 60, 309, 345, 447, 753, 756, 858, 870
Sau3AI GATC 1 cut(s) 331
Sau96I GGNCC 2 cut(s) 248, 793
ScrFI CCNGG 5 cut(s) 25, 123, 241, 246, 409
SduI GDGCHC 6 cut(s) 10, 101, 172, 485, 567, 575
SfaNI GCATC 2 cut(s) 22, 394
SfuI TTCGAA 1 cut(s) 490
SinI GGWCC 2 cut(s) 248, 793
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
SphI GCATGC 1 cut(s) 308
Sse9I AATT 7 cut(s) 252, 285, 336, 804, 820, 828, 879
SspMI CTAG 2 cut(s) 384, 401
StyD4I CCNGG 5 cut(s) 23, 121, 239, 244, 407
TaaI ACNGT 4 cut(s) 421, 469, 583, 625
TaqI TCGA 1 cut(s) 490
TasI AATT 7 cut(s) 252, 285, 336, 804, 820, 828, 879
TatI WGTACW 1 cut(s) 642
TfiI GAWTC 2 cut(s) 181, 532
Tru1I TTAA 2 cut(s) 477, 627
Tru9I TTAA 2 cut(s) 477, 627
TscAI CASTG 1 cut(s) 474
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 9 cut(s) 49, 59, 308, 344, 446, 752, 755, 857, 869
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 6 cut(s) 278, 580, 620, 648, 754, 854
TspRI CASTG 1 cut(s) 474
Vha464I CTTAAG 1 cut(s) 476
VneI GTGCAC 2 cut(s) 563, 571
VpaK11BI GGWCC 2 cut(s) 248, 793
XbaI TCTAGA 2 cut(s) 383, 400
XceI RCATGY 3 cut(s) 140, 308, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 804
XmiI GTMKAC 1 cut(s) 585
XspI CTAG 2 cut(s) 384, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.