Rorug03G0146400

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0015657
Physical Location & Seq
Reverse (-)
11981043 .. 11986920
5878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0146400.1

Sequence Viewer

Length: 1599 bp
ATGCGGACCAACAGAGGAAAGATCCCAAAGCAGAACTCGTGGCCTGGAGCAGTAGTGAGGAAATGGCTCAACATTCCGACCGGAGGGGACGAGTTTCATTCCGATTACTCTGTAAAAAATGTAACTGAGAGTAGGAGGAAGAGTTGCTCGGACCAGGACCACTACGTCGTTGTACCGGACGATGTTGCAGATGGTTGGTTGATGGAGGACGCTTACGGCCCAAGTTTTGCGCCGGAGGCACCAGCTGTGACCGACCATCTCAACCTCAGAATGTTCGTGGGGACATGGAATGTTGGAGGCAAATCACCACAAGAGGACTTGAACTTAAGGGAATGGCTTAGGTCCCCAACTCCTGCCGATATCTATGTTCTTGGGTTCCAGGAAATTGTCCCTTTAAACGCCGGTAATGTATTGGGTGCGGAGGACAAAGGCCCAGCTGACAAGTGGCTTTCCCTTATTCGAGATGCTCTTAACAAGAATGACCCCGGACTTCCTAATAACTACAACAATGCCACCCATAATGATCAGCAAGCACATCTCGATCAGCAAGGCAGCATGATGCCAAGGCTCAGCTTCTCGGACTTGCTTTCCTTAGAAGATGAACTCAGTACTGCGGACTTCGAGACACTGCTGAATTTGAATCCCGCGTCAACTTCTAGTGGAGAAAGCTCACCGAGCTCCCCACTGGGGCTGGGTATGCAGGAGAGTACTTCAATGCAACGACATCGTTATTGCTTAGCAGCGAGCAAGCAGATGGTGGGAATTTTCTTGTGTGTGTGGGTTCGCGCTGATCTTTGTAGACACATTAGCAACATGAAGGTCTCATGTGTTGGTCGAGGCATAATGGGATATCTTGGAAATAAGGGTTCAATATCAATAAGCATGACATTGCATAAAACAACCTTTTGCTTTGTCTGTGCTCACTTAACTTCTGGTGAGAAAGAAGGGGATGAGATGAAAAGGAATTCAGATGTCATCGAAATTTTAAAGAAGACAAAGTTTTCTAACTCATGTAGAACACGACCACTTCCTCCTGATAGCATATTGGACCATGACAAGATTATTTGGCTTGGGGATTTAAATTACCGGCTAGCAGCTGCTTGCGCTGACACGCATGAGCTTCTGCAGAAGCAGCATTGGCAAGCACTTTTAGAGATAGATCAGCTAAGAATAGAGCAACAAGCTGGTAGAGTATTTAAAGGGTGGGAAGAAGGGAGGATATATTTTGCTCCAACTTACAAATACCTTGCAAATTCTAATCATTATGTTGCCCAAACTTCGAATTCTAGAGAGAAGAGACGCACCCCTGCCTGGTGTGACAGGATTCTATGGAAAGGAGAAGGACTTAAACAAATGTGGTATTTGAGAGGGGAGTCAAAGTTCTCGGACCACAGACCGGTCTATTCTTTGTTTGCGGTCCAAATAAACTTGGCCAGCAAGAAAAAACCCAAACCTGCTGTTCCCATAAGCACAACAAGCAACAGAGTTGATCCAAATTCTCGGGTGCCAGCGACATGCGTGGCCAAAGTGCAAGCCGAGGAGCTTTTGGTACTCACAAGAGCAAAAAGCTGCTTAGACACAAGCTCCTCCAGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

532

Amino Acids

59.66

Weight (kDa)

8.33

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos2 PF22669 94 - 168 1.9e-08 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos2 PF22669 239 - 469 6.8e-62 Endonuclease/Exonuclease/phosphatase family 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 1462
AccB1I GGYRCC 2 cut(s) 238, 1504
AccI GTMKAC 1 cut(s) 799
AccII CGCG 2 cut(s) 647, 786
AciI CCGC 5 cut(s) 4, 419, 614, 645, 1415
AclWI GGATC 2 cut(s) 16, 1484
AcoI YGGCCR 2 cut(s) 1431, 1521
AcsI RAATTY 7 cut(s) 634, 762, 964, 981, 1252, 1282, 1495
AfaI GTAC 4 cut(s) 174, 610, 709, 1551
AfiI CCNNNNNNNGG 4 cut(s) 83, 687, 1311, 1396
AflII CTTAAG 1 cut(s) 325
AgeI ACCGGT 1 cut(s) 1396
AgsI TTSAA 4 cut(s) 322, 640, 714, 870
AjnI CCWGG 5 cut(s) 43, 153, 378, 1310, 1589
AloI GAACNNNNNNTCC 2 cut(s) 1364, 1396
Alw21I GWGCWC 2 cut(s) 680, 922
Alw26I GTCTC 3 cut(s) 617, 826, 1291
AlwI GGATC 2 cut(s) 16, 1484
AlwNI CAGNNNCTG 1 cut(s) 1596
Ama87I CYCGRG 1 cut(s) 1500
AoxI GGCC 5 cut(s) 41, 217, 430, 1431, 1521
ApeKI GCWGC 6 cut(s) 552, 740, 1094, 1097, 1132, 1569
ApoI RAATTY 7 cut(s) 634, 762, 964, 981, 1252, 1282, 1495
AsiGI ACCGGT 1 cut(s) 1396
AspLEI GCGC 3 cut(s) 232, 788, 1106
AspS9I GGNCC 9 cut(s) 6, 151, 157, 218, 342, 431, 1048, 1387, 1417
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 3 cut(s) 297, 663, 947
AsuII TTCGAA 1 cut(s) 1280
AsuNHI GCTAGC 1 cut(s) 1090
AvaI CYCGRG 1 cut(s) 1500
AvaII GGWCC 7 cut(s) 6, 151, 157, 342, 1048, 1387, 1417
BalI TGGCCA 2 cut(s) 1433, 1523
BanI GGYRCC 2 cut(s) 238, 1504
BanII GRGCYC 1 cut(s) 680
BauI CACGAG 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 998
Bbv12I GWGCWC 2 cut(s) 680, 922
BbvI GCAGC 6 cut(s) 564, 752, 1084, 1106, 1144, 1556
BccI CCATC 4 cut(s) 185, 196, 264, 748
BceAI ACGGC 1 cut(s) 232
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 5 cut(s) 45, 155, 380, 1312, 1591
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 1 cut(s) 486
BcoDI GTCTC 3 cut(s) 617, 826, 1291
BfaI CTAG 3 cut(s) 657, 1091, 1287
BfmI CTRYAG 1 cut(s) 1124
BfrI CTTAAG 1 cut(s) 325
BfuAI ACCTGC 1 cut(s) 1462
BisI GCNGC 6 cut(s) 553, 741, 1095, 1098, 1133, 1570
BlpI GCTNAGC 2 cut(s) 569, 736
BlsI GCNGC 6 cut(s) 554, 742, 1096, 1099, 1134, 1571
BmcAI AGTACT 2 cut(s) 610, 709
Bme1390I CCNGG 6 cut(s) 45, 155, 380, 486, 1312, 1591
Bme18I GGWCC 7 cut(s) 6, 151, 157, 342, 1048, 1387, 1417
BmeT110I CYCGRG 1 cut(s) 1500
BmgT120I GGNCC 9 cut(s) 6, 151, 157, 218, 342, 431, 1048, 1387, 1417
BmiI GGNNCC 4 cut(s) 240, 344, 377, 1506
BmrFI CCNGG 6 cut(s) 45, 155, 380, 486, 1312, 1591
BmrI ACTGGG 1 cut(s) 695
BmsI GCATC 2 cut(s) 454, 549
BmtI GCTAGC 1 cut(s) 1094
BmuI ACTGGG 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 998
BpmI CTGGAG 2 cut(s) 66, 1573
Bpu10I CCTNAGC 1 cut(s) 338
Bpu1102I GCTNAGC 2 cut(s) 569, 736
Bpu14I TTCGAA 1 cut(s) 1280
BpuMI CCSGG 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 826
BsaJI CCNNGG 3 cut(s) 484, 563, 1536
BsaWI WCCGGW 3 cut(s) 80, 175, 1396
BsaXI ACNNNNNCTCC 8 cut(s) 39, 69, 127, 157, 1364, 1394, 1568, 1598
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 687, 1311, 1396
Bse118I RCCGGY 3 cut(s) 401, 1086, 1396
Bse1I ACTGG 1 cut(s) 690
Bse3DI GCAATG 1 cut(s) 887
BseBI CCWGG 5 cut(s) 45, 155, 380, 1312, 1591
BseDI CCNNGG 3 cut(s) 484, 563, 1536
BseGI GGATG 1 cut(s) 955
BseLI CCNNNNNNNGG 4 cut(s) 83, 687, 1311, 1396
BseMI GCAATG 1 cut(s) 887
BseMII CTCAG 4 cut(s) 117, 280, 583, 619
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 2 cut(s) 1553, 1576
BseXI GCAGC 6 cut(s) 564, 752, 1084, 1106, 1144, 1556
BseYI CCCAGC 2 cut(s) 433, 691
Bsh1236I CGCG 2 cut(s) 647, 786
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 5 cut(s) 43, 219, 432, 1433, 1523
BshNI GGYRCC 2 cut(s) 238, 1504
BshTI ACCGGT 1 cut(s) 1396
BsiEI CGRYCG 1 cut(s) 81
BsiHKAI GWGCWC 2 cut(s) 680, 922
BsiHKCI CYCGRG 1 cut(s) 1500
BsiSI CCGG 7 cut(s) 81, 176, 233, 402, 486, 1087, 1397
BslFI GGGAC 4 cut(s) 101, 295, 328, 374
BslI CCNNNNNNNGG 4 cut(s) 83, 687, 1311, 1396
BsmAI GTCTC 3 cut(s) 617, 826, 1291
BsmBI CGTCTC 1 cut(s) 1291
BsmFI GGGAC 4 cut(s) 101, 295, 328, 374
BsnI GGCC 5 cut(s) 43, 219, 432, 1433, 1523
Bso31I GGTCTC 1 cut(s) 826
BsoBI CYCGRG 1 cut(s) 1500
Bsp119I TTCGAA 1 cut(s) 1280
Bsp1286I GDGCHC 2 cut(s) 680, 922
Bsp143I GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
Bsp1720I GCTNAGC 2 cut(s) 569, 736
BspACI CCGC 5 cut(s) 4, 419, 614, 645, 1415
BspANI GGCC 5 cut(s) 43, 219, 432, 1433, 1523
BspCNI CTCAG 4 cut(s) 118, 279, 582, 618
BspFNI CGCG 2 cut(s) 647, 786
BspLI GGNNCC 4 cut(s) 240, 344, 377, 1506
BspMAI CTGCAG 1 cut(s) 1128
BspMI ACCTGC 1 cut(s) 1462
BspOI GCTAGC 1 cut(s) 1094
BspPI GGATC 2 cut(s) 16, 1484
BspT104I TTCGAA 1 cut(s) 1280
BspT107I GGYRCC 2 cut(s) 238, 1504
BspTI CTTAAG 1 cut(s) 325
BspTNI GGTCTC 1 cut(s) 826
BsrDI GCAATG 1 cut(s) 887
BsrFI RCCGGY 3 cut(s) 401, 1086, 1396
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 3 cut(s) 401, 1086, 1396
BssECI CCNNGG 3 cut(s) 484, 563, 1536
BssMI GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
BssSI CACGAG 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 563
Bst2BI CACGAG 1 cut(s) 37
Bst2UI CCWGG 5 cut(s) 45, 155, 380, 1312, 1591
Bst6I CTCTTC 2 cut(s) 134, 1289
BstAFI CTTAAG 1 cut(s) 325
BstBI TTCGAA 1 cut(s) 1280
BstC8I GCNNGC 9 cut(s) 531, 745, 749, 1092, 1102, 1143, 1435, 1509, 1533
BstDEI CTNAG 9 cut(s) 126, 266, 338, 569, 592, 605, 736, 1166, 1573
BstF5I GGATG 1 cut(s) 955
BstFNI CGCG 2 cut(s) 647, 786
BstHHI GCGC 3 cut(s) 232, 788, 1106
BstKTI GATC 6 cut(s) 24, 526, 544, 793, 1162, 1492
BstMAI GTCTC 3 cut(s) 617, 826, 1291
BstMBI GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 7 cut(s) 236, 675, 697, 1103, 1132, 1138, 1476
BstNI CCWGG 5 cut(s) 45, 155, 380, 1312, 1591
BstNSI RCATGY 1 cut(s) 1518
BstSCI CCNGG 6 cut(s) 43, 153, 378, 484, 1310, 1589
BstSFI CTRYAG 1 cut(s) 1124
BstUI CGCG 2 cut(s) 647, 786
BstV1I GCAGC 6 cut(s) 564, 752, 1084, 1106, 1144, 1556
BstV2I GAAGAC 1 cut(s) 998
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BsuRI GGCC 5 cut(s) 43, 219, 432, 1433, 1523
BtsCI GGATG 1 cut(s) 955
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 2 cut(s) 626, 683
BveI ACCTGC 1 cut(s) 1462
Cac8I GCNNGC 9 cut(s) 531, 745, 749, 1092, 1102, 1143, 1435, 1509, 1533
CaiI CAGNNNCTG 1 cut(s) 1596
CfoI GCGC 3 cut(s) 232, 788, 1106
Cfr10I RCCGGY 3 cut(s) 401, 1086, 1396
Cfr13I GGNCC 9 cut(s) 6, 151, 157, 218, 342, 431, 1048, 1387, 1417
CseI GACGC 3 cut(s) 218, 636, 1308
Csp6I GTAC 4 cut(s) 173, 609, 708, 1550
CspAI ACCGGT 1 cut(s) 1396
CviAII CATG 9 cut(s) 285, 556, 814, 825, 883, 1011, 1052, 1115, 1515
CviQI GTAC 4 cut(s) 173, 609, 708, 1550
DdeI CTNAG 9 cut(s) 126, 266, 338, 569, 592, 605, 736, 1166, 1573
DpnI GATC 6 cut(s) 23, 525, 543, 792, 1161, 1491
DpnII GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
DraI TTTAAA 4 cut(s) 396, 987, 1080, 1198
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 2 cut(s) 1431, 1521
Eam1104I CTCTTC 2 cut(s) 134, 1289
EarI CTCTTC 2 cut(s) 134, 1289
Ecl136II GAGCTC 1 cut(s) 678
Eco130I CCWWGG 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 680
Eco31I GGTCTC 1 cut(s) 826
Eco32I GATATC 2 cut(s) 361, 851
Eco47I GGWCC 7 cut(s) 6, 151, 157, 342, 1048, 1387, 1417
Eco53kI GAGCTC 1 cut(s) 678
Eco88I CYCGRG 1 cut(s) 1500
EcoICRI GAGCTC 1 cut(s) 678
EcoO109I RGGNCCY 1 cut(s) 342
EcoRI GAATTC 2 cut(s) 964, 1282
EcoRII CCWGG 5 cut(s) 43, 153, 378, 1310, 1589
EcoRV GATATC 2 cut(s) 361, 851
EcoT14I CCWWGG 1 cut(s) 563
EcoT38I GRGCYC 1 cut(s) 680
ErhI CCWWGG 1 cut(s) 563
Esp3I CGTCTC 1 cut(s) 1291
FaeI CATG 9 cut(s) 288, 559, 817, 828, 886, 1014, 1055, 1118, 1518
FaqI GGGAC 4 cut(s) 101, 295, 328, 374
FatI CATG 9 cut(s) 284, 555, 813, 824, 882, 1010, 1051, 1114, 1514
FauI CCCGC 1 cut(s) 652
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 1 cut(s) 799
Fnu4HI GCNGC 6 cut(s) 553, 741, 1095, 1098, 1133, 1570
FokI GGATG 1 cut(s) 962
FriOI GRGCYC 1 cut(s) 680
Fsp4HI GCNGC 6 cut(s) 553, 741, 1095, 1098, 1133, 1570
FspBI CTAG 3 cut(s) 657, 1091, 1287
GlaI GCGC 3 cut(s) 231, 787, 1105
GluI GCNGC 6 cut(s) 553, 741, 1095, 1098, 1133, 1570
GsaI CCCAGC 2 cut(s) 437, 695
GsuI CTGGAG 2 cut(s) 66, 1573
HaeIII GGCC 5 cut(s) 43, 219, 432, 1433, 1523
HapII CCGG 7 cut(s) 81, 176, 233, 402, 486, 1087, 1397
HgaI GACGC 3 cut(s) 218, 636, 1308
HhaI GCGC 3 cut(s) 232, 788, 1106
Hin1II CATG 9 cut(s) 288, 559, 817, 828, 886, 1014, 1055, 1118, 1518
Hin6I GCGC 3 cut(s) 230, 786, 1104
HinP1I GCGC 3 cut(s) 230, 786, 1104
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HinfI GANTC 3 cut(s) 640, 1324, 1373
HpaII CCGG 7 cut(s) 81, 176, 233, 402, 486, 1087, 1397
HphI GGTGA 3 cut(s) 297, 663, 947
Hpy166II GTNNAC 2 cut(s) 651, 800
Hpy188I TCNGA 8 cut(s) 78, 103, 151, 269, 580, 970, 1387, 1598
Hpy188III TCNNGA 5 cut(s) 461, 539, 622, 1034, 1287
Hpy8I GTNNAC 2 cut(s) 651, 800
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 4 cut(s) 811, 938, 1205, 1334
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 7 cut(s) 188, 700, 718, 892, 1126, 1250, 1531
HpyF10VI GCNNNNNNNGC 7 cut(s) 236, 675, 697, 1103, 1132, 1138, 1476
HpyF3I CTNAG 9 cut(s) 126, 266, 338, 569, 592, 605, 736, 1166, 1573
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 9 cut(s) 288, 559, 817, 828, 886, 1014, 1055, 1118, 1518
HspAI GCGC 3 cut(s) 230, 786, 1104
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
LmnI GCTCC 5 cut(s) 47, 683, 1234, 1540, 1589
Lsp1109I GCAGC 6 cut(s) 564, 752, 1084, 1106, 1144, 1556
LweI GCATC 2 cut(s) 454, 549
MaeI CTAG 3 cut(s) 657, 1091, 1287
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 3 cut(s) 121, 247, 1316
MalI GATC 6 cut(s) 23, 525, 543, 792, 1161, 1491
MboI GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
MboII GAAGA 5 cut(s) 151, 608, 1003, 1220, 1306
MflI RGATCY 1 cut(s) 21
MhlI GDGCHC 2 cut(s) 680, 922
MlsI TGGCCA 2 cut(s) 1433, 1523
MluCI AATT 9 cut(s) 384, 634, 762, 964, 981, 1081, 1252, 1282, 1495
MluNI TGGCCA 2 cut(s) 1433, 1523
MlyI GAGTC 1 cut(s) 1382
MmeI TCCRAC 3 cut(s) 101, 274, 1256
Mox20I TGGCCA 2 cut(s) 1433, 1523
MscI TGGCCA 2 cut(s) 1433, 1523
MseI TTAA 8 cut(s) 326, 395, 471, 926, 986, 1079, 1197, 1347
Msp20I TGGCCA 2 cut(s) 1433, 1523
MspA1I CMGCKG 3 cut(s) 245, 437, 1097
MspCI CTTAAG 1 cut(s) 325
MspI CCGG 7 cut(s) 81, 176, 233, 402, 486, 1087, 1397
MspR9I CCNGG 6 cut(s) 45, 155, 380, 486, 1312, 1591
MvaI CCWGG 5 cut(s) 45, 155, 380, 1312, 1591
MvnI CGCG 2 cut(s) 647, 786
MwoI GCNNNNNNNGC 7 cut(s) 236, 675, 697, 1103, 1132, 1138, 1476
NciI CCSGG 1 cut(s) 486
NdeII GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
NheI GCTAGC 1 cut(s) 1090
NlaIII CATG 9 cut(s) 288, 559, 817, 828, 886, 1014, 1055, 1118, 1518
NlaIV GGNNCC 4 cut(s) 240, 344, 377, 1506
NmeAIII GCCGAG 1 cut(s) 1561
NmuCI GTSAC 2 cut(s) 247, 1316
NspI RCATGY 1 cut(s) 1518
NspV TTCGAA 1 cut(s) 1280
PfeI GAWTC 2 cut(s) 640, 1324
PfoI TCCNGGA 1 cut(s) 378
PinAI ACCGGT 1 cut(s) 1396
PkrI GCNGC 6 cut(s) 554, 742, 1096, 1099, 1134, 1571
PleI GAGTC 1 cut(s) 1381
PpsI GAGTC 1 cut(s) 1381
PpuMI RGGWCCY 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 680
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 5 cut(s) 43, 153, 378, 1310, 1589
PspFI CCCAGC 2 cut(s) 433, 691
PspGI CCWGG 5 cut(s) 43, 153, 378, 1310, 1589
PspN4I GGNNCC 4 cut(s) 240, 344, 377, 1506
PspPI GGNCC 9 cut(s) 6, 151, 157, 218, 342, 431, 1048, 1387, 1417
PspPPI RGGWCCY 1 cut(s) 342
PstI CTGCAG 1 cut(s) 1128
PstNI CAGNNNCTG 1 cut(s) 1596
PsuI RGATCY 1 cut(s) 21
PvuII CAGCTG 3 cut(s) 245, 437, 1097
RsaI GTAC 4 cut(s) 174, 610, 709, 1551
RsaNI GTAC 4 cut(s) 173, 609, 708, 1550
SacI GAGCTC 1 cut(s) 680
SaqAI TTAA 8 cut(s) 326, 395, 471, 926, 986, 1079, 1197, 1347
SatI GCNGC 6 cut(s) 553, 741, 1095, 1098, 1133, 1570
Sau3AI GATC 6 cut(s) 21, 523, 541, 790, 1159, 1489
Sau96I GGNCC 9 cut(s) 6, 151, 157, 218, 342, 431, 1048, 1387, 1417
ScaI AGTACT 2 cut(s) 610, 709
SchI GAGTC 1 cut(s) 1382
ScrFI CCNGG 6 cut(s) 45, 155, 380, 486, 1312, 1591
SduI GDGCHC 2 cut(s) 680, 922
SfaNI GCATC 2 cut(s) 454, 549
SfcI CTRYAG 1 cut(s) 1124
SfuI TTCGAA 1 cut(s) 1280
SinI GGWCC 7 cut(s) 6, 151, 157, 342, 1048, 1387, 1417
SmiI ATTTAAAT 1 cut(s) 1080
SmlI CTYRAG 1 cut(s) 325
SmoI CTYRAG 1 cut(s) 325
Sse9I AATT 9 cut(s) 384, 634, 762, 964, 981, 1081, 1252, 1282, 1495
SsiI CCGC 5 cut(s) 4, 419, 614, 645, 1415
SspMI CTAG 3 cut(s) 657, 1091, 1287
SstI GAGCTC 1 cut(s) 680
StyD4I CCNGG 6 cut(s) 43, 153, 378, 484, 1310, 1589
StyI CCWWGG 1 cut(s) 563
SwaI ATTTAAAT 1 cut(s) 1080
TaiI ACGT 1 cut(s) 168
TaqI TCGA 6 cut(s) 460, 540, 621, 835, 978, 1280
TaqII GACCGA 1 cut(s) 266
TasI AATT 9 cut(s) 384, 634, 762, 964, 981, 1081, 1252, 1282, 1495
TatI WGTACW 2 cut(s) 608, 707
TfiI GAWTC 2 cut(s) 640, 1324
Tru1I TTAA 8 cut(s) 326, 395, 471, 926, 986, 1079, 1197, 1347
Tru9I TTAA 8 cut(s) 326, 395, 471, 926, 986, 1079, 1197, 1347
TscAI CASTG 2 cut(s) 633, 690
TseFI GTSAC 2 cut(s) 247, 1316
TseI GCWGC 6 cut(s) 552, 740, 1094, 1097, 1132, 1569
Tsp45I GTSAC 2 cut(s) 247, 1316
TspDTI ATGAA 4 cut(s) 86, 615, 830, 971
TspRI CASTG 2 cut(s) 633, 690
Vha464I CTTAAG 1 cut(s) 325
VpaK11BI GGWCC 7 cut(s) 6, 151, 157, 342, 1048, 1387, 1417
XapI RAATTY 7 cut(s) 634, 762, 964, 981, 1252, 1282, 1495
XbaI TCTAGA 1 cut(s) 1286
XceI RCATGY 1 cut(s) 1518
XcmI CCANNNNNNNNNTGG 1 cut(s) 441
XmiI GTMKAC 1 cut(s) 799
XspI CTAG 3 cut(s) 657, 1091, 1287
ZrmI AGTACT 2 cut(s) 610, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.