Rh3BG226300

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0015657
Physical Location & Seq
Reverse (-)
20670299 .. 20673327
3029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG226300.1

Sequence Viewer

Length: 990 bp
ATGGATCTTCTTGTTTGTAAGGAATTGGTTGAGTGGGAGCACAAGAGCAAGAACCCCGGGAAGATGCATGCTTGTGGCCATGATGTTCATGTAACCATGCTGCTTGGAGCTGCTAAGTTACTTCAGCTCAAGAGTAAACAATTAAAGGGCACAGTCAAGCTTGTTTTTCAACCTGCAGAGGAGGGTCATGCAGGGGCATACCATATGTTAAAAGAGGTTGCTCTTGATGGAATTCAAGGCATTTTTGGATTACATGTTTCGCCAGAAATGCAGACTGGTTCAATTGGTTCTAGACCTGGTCCATTTCTTGCTGGTGCTGGTAGATTTTTAGTCACAGTTCAAGGAAAAGGTGGACATGCTGCAGCTCCCCATTTGACTGCAGACCCAATTCTTGCAGCCTGCTCGGTCATCCTTGCGCTCCAACAGATTGTATCTCGAGAAACCGACCCTCTAGAGGCCAGGGTGGTAACAGTTGGGTTTGTTAACGGTGGCCAAGCCGCAAATGTCATTCCAGAGACGGTGGAACTTAAAGGGACTTTTCGAAGCTTGACTTTAGATGGTATACATTACCTTCAGCAAAGGATTAAGGAGGTGATAGAGATGCAAGCATCAGTGCATCAGTGTAATGCAACAGTGGACTTCATGCATGAAAAAATGAGGCCGTATCCACCAACTGTAATTGATGAAGCACTCTATGTACACAGCAAGAGTGTTAGTGAAGCCTTGGTTGGGGAAGCAAATGTGTATCTTCTTCCAATGACTATGGGAGCAGAGGACTTTAGCTTCTATACACATAAAATGCCAGCCGCAATGTTCATGATCGGGACTACAGACCATACCTTGGGATCGAATGCTACAAGTTTGCATTCACCGTACTTGGTCATTGATGAGGAGGTACTTCCAATAGGAGCAGCTCTTCATGCTGCAGTTGCGATCTCGTTCTTGGATAACAATACTGATGCTGCTGCTGCGCACTCAGTCATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

329

Amino Acids

35.33

Weight (kDa)

5.99

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 1 - 311 1.3e-36 Peptidase family M20/M25/M40
M20_dimer PF07687 105 - 201 1.7e-13 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 972
Acc36I ACCTGC 1 cut(s) 181
AccB7I CCANNNNNTGG 1 cut(s) 841
AccI GTMKAC 1 cut(s) 562
AciI CCGC 2 cut(s) 498, 807
AclWI GGATC 2 cut(s) 12, 853
AcoI YGGCCR 2 cut(s) 76, 490
AcsI RAATTY 1 cut(s) 231
AcuI CTGAAG 2 cut(s) 107, 557
AfaI GTAC 3 cut(s) 699, 875, 897
AfiI CCNNNNNNNGG 3 cut(s) 454, 729, 841
AflIII ACRYGT 1 cut(s) 253
AgsI TTSAA 4 cut(s) 170, 236, 282, 341
AjnI CCWGG 2 cut(s) 295, 458
AjuI GAANNNNNNNTTGG 2 cut(s) 711, 743
AluBI AGCT 7 cut(s) 110, 127, 160, 365, 546, 783, 914
AluI AGCT 7 cut(s) 110, 127, 160, 365, 546, 783, 914
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 509
AlwI GGATC 2 cut(s) 12, 853
Ama87I CYCGRG 2 cut(s) 56, 435
AoxI GGCC 4 cut(s) 76, 456, 490, 659
ApoI RAATTY 1 cut(s) 231
AspLEI GCGC 2 cut(s) 418, 973
AspS9I GGNCC 1 cut(s) 299
AsuC2I CCSGG 2 cut(s) 57, 58
AsuHPI GGTGA 2 cut(s) 604, 861
AsuII TTCGAA 1 cut(s) 541
AvaI CYCGRG 2 cut(s) 56, 435
AvaII GGWCC 1 cut(s) 299
BaeGI GKGCMC 1 cut(s) 152
BalI TGGCCA 2 cut(s) 78, 492
Bbv12I GWGCWC 1 cut(s) 42
BccI CCATC 2 cut(s) 221, 551
BceAI ACGGC 1 cut(s) 646
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BciT130I CCWGG 2 cut(s) 297, 460
BciVI GTATCC 1 cut(s) 675
BcnI CCSGG 2 cut(s) 57, 58
BcoDI GTCTC 1 cut(s) 509
BfaI CTAG 3 cut(s) 291, 452, 988
BfmI CTRYAG 5 cut(s) 174, 360, 378, 828, 924
BfuAI ACCTGC 1 cut(s) 181
BfuI GTATCC 1 cut(s) 675
Bme1390I CCNGG 4 cut(s) 57, 58, 297, 460
Bme18I GGWCC 1 cut(s) 299
BmeT110I CYCGRG 2 cut(s) 56, 435
BmgT120I GGNCC 1 cut(s) 299
BmrFI CCNGG 4 cut(s) 57, 58, 297, 460
BmsI GCATC 5 cut(s) 54, 591, 617, 625, 949
Bpu14I TTCGAA 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 2 cut(s) 57, 58
BsaJI CCNNGG 5 cut(s) 55, 56, 459, 723, 840
Bsc4I CCNNNNNNNGG 3 cut(s) 454, 729, 841
Bse1I ACTGG 1 cut(s) 280
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 2 cut(s) 297, 460
BseDI CCNNGG 5 cut(s) 55, 56, 459, 723, 840
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 454, 729, 841
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 1 cut(s) 990
BseNI ACTGG 1 cut(s) 280
BseRI GAGGAG 2 cut(s) 194, 905
BseSI GKGCMC 1 cut(s) 152
BshFI GGCC 4 cut(s) 78, 458, 492, 661
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 2 cut(s) 56, 435
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 2 cut(s) 547, 838
BslI CCNNNNNNNGG 3 cut(s) 454, 729, 841
BsmAI GTCTC 1 cut(s) 509
BsmBI CGTCTC 1 cut(s) 509
BsmFI GGGAC 2 cut(s) 547, 838
BsmI GAATGC 2 cut(s) 856, 865
BsnI GGCC 4 cut(s) 78, 458, 492, 661
BsoBI CYCGRG 2 cut(s) 56, 435
Bsp119I TTCGAA 1 cut(s) 541
Bsp1286I GDGCHC 2 cut(s) 42, 152
Bsp1407I TGTACA 1 cut(s) 697
Bsp143I GATC 4 cut(s) 4, 819, 845, 933
BspACI CCGC 2 cut(s) 498, 807
BspANI GGCC 4 cut(s) 78, 458, 492, 661
BspCNI CTCAG 1 cut(s) 989
BspHI TCATGA 1 cut(s) 816
BspMAI CTGCAG 4 cut(s) 178, 364, 382, 928
BspMI ACCTGC 1 cut(s) 181
BspPI GGATC 2 cut(s) 12, 853
BspQI GCTCTTC 1 cut(s) 921
BspT104I TTCGAA 1 cut(s) 541
BsrDI GCAATG 1 cut(s) 816
BsrGI TGTACA 1 cut(s) 697
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 5 cut(s) 55, 56, 459, 723, 840
BssMI GATC 4 cut(s) 4, 819, 845, 933
BssNAI GTATAC 1 cut(s) 563
BssT1I CCWWGG 2 cut(s) 723, 840
Bst1107I GTATAC 1 cut(s) 563
Bst2UI CCWGG 2 cut(s) 297, 460
Bst4CI ACNGT 8 cut(s) 154, 337, 472, 488, 520, 634, 676, 873
Bst6I CTCTTC 1 cut(s) 921
BstAUI TGTACA 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 541
BstC8I GCNNGC 4 cut(s) 69, 400, 606, 804
BstDEI CTNAG 2 cut(s) 114, 976
BstF5I GGATG 1 cut(s) 408
BstHHI GCGC 2 cut(s) 418, 973
BstKTI GATC 4 cut(s) 7, 822, 848, 936
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 4 cut(s) 4, 819, 845, 933
BstMWI GCNNNNNNNGC 4 cut(s) 268, 920, 929, 968
BstNI CCWGG 2 cut(s) 297, 460
BstNSI RCATGY 3 cut(s) 71, 257, 359
BstSCI CCNGG 4 cut(s) 55, 56, 295, 458
BstSFI CTRYAG 5 cut(s) 174, 360, 378, 828, 924
BstSLI GKGCMC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 563
BsuI GTATCC 1 cut(s) 675
BsuRI GGCC 4 cut(s) 78, 458, 492, 661
BtsCI GGATG 1 cut(s) 408
BtsIMutI CAGTG 3 cut(s) 618, 626, 639
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 4 cut(s) 69, 400, 606, 804
CciI TCATGA 1 cut(s) 816
CfoI GCGC 2 cut(s) 418, 973
Cfr13I GGNCC 1 cut(s) 299
Cfr9I CCCGGG 1 cut(s) 56
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 3 cut(s) 698, 874, 896
CviQI GTAC 3 cut(s) 698, 874, 896
DdeI CTNAG 2 cut(s) 114, 976
DpnI GATC 4 cut(s) 6, 821, 847, 935
DpnII GATC 4 cut(s) 4, 819, 845, 933
EaeI YGGCCR 2 cut(s) 76, 490
Eam1104I CTCTTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 921
Eco130I CCWWGG 2 cut(s) 723, 840
Eco47I GGWCC 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 107, 557
Eco88I CYCGRG 2 cut(s) 56, 435
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 295, 458
EcoT14I CCWWGG 2 cut(s) 723, 840
EcoT22I ATGCAT 2 cut(s) 69, 648
ErhI CCWWGG 2 cut(s) 723, 840
Esp3I CGTCTC 1 cut(s) 509
FalI AAGNNNNNCTT 2 cut(s) 535, 567
FaqI GGGAC 2 cut(s) 547, 838
FauNDI CATATG 1 cut(s) 204
FblI GTMKAC 1 cut(s) 562
FokI GGATG 1 cut(s) 395
FspBI CTAG 3 cut(s) 291, 452, 988
FspI TGCGCA 1 cut(s) 972
GlaI GCGC 2 cut(s) 417, 972
HaeIII GGCC 4 cut(s) 78, 458, 492, 661
HapII CCGG 1 cut(s) 57
HhaI GCGC 2 cut(s) 418, 973
Hin6I GCGC 2 cut(s) 416, 971
HinP1I GCGC 2 cut(s) 416, 971
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HindIII AAGCTT 2 cut(s) 158, 544
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 1 cut(s) 57
HphI GGTGA 2 cut(s) 604, 861
Hpy166II GTNNAC 6 cut(s) 137, 353, 484, 563, 637, 700
Hpy188III TCNNGA 9 cut(s) 130, 224, 291, 435, 437, 452, 512, 817, 823
Hpy8I GTNNAC 6 cut(s) 137, 353, 484, 563, 637, 700
HpyAV CCTTC 1 cut(s) 581
HpyCH4III ACNGT 8 cut(s) 154, 337, 472, 488, 520, 634, 676, 873
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 920, 929, 968
HpyF3I CTNAG 2 cut(s) 114, 976
HspAI GCGC 2 cut(s) 416, 971
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 4 cut(s) 4, 819, 845, 933
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 6 cut(s) 37, 107, 370, 423, 767, 908
LweI GCATC 5 cut(s) 54, 591, 617, 625, 949
MabI ACCWGGT 1 cut(s) 295
MaeI CTAG 3 cut(s) 291, 452, 988
MaeIII GTNAC 4 cut(s) 91, 117, 331, 466
MalI GATC 4 cut(s) 6, 821, 847, 935
MboI GATC 4 cut(s) 4, 819, 845, 933
MboII GAAGA 4 cut(s) 73, 740, 743, 908
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 42, 152
MlsI TGGCCA 2 cut(s) 78, 492
MluCI AATT 6 cut(s) 23, 140, 231, 282, 387, 678
MluNI TGGCCA 2 cut(s) 78, 492
MmeI TCCRAC 1 cut(s) 445
Mox20I TGGCCA 2 cut(s) 78, 492
Mph1103I ATGCAT 2 cut(s) 69, 648
MscI TGGCCA 2 cut(s) 78, 492
MseI TTAA 5 cut(s) 143, 209, 483, 528, 585
MslI CAYNNNNRTG 1 cut(s) 72
Msp20I TGGCCA 2 cut(s) 78, 492
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 4 cut(s) 57, 58, 297, 460
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 2 cut(s) 856, 865
MvaI CCWGG 2 cut(s) 297, 460
MwoI GCNNNNNNNGC 4 cut(s) 268, 920, 929, 968
NciI CCSGG 2 cut(s) 57, 58
NdeI CATATG 1 cut(s) 204
NdeII GATC 4 cut(s) 4, 819, 845, 933
NmuCI GTSAC 1 cut(s) 331
NsbI TGCGCA 1 cut(s) 972
NsiI ATGCAT 2 cut(s) 69, 648
NspI RCATGY 3 cut(s) 71, 257, 359
NspV TTCGAA 1 cut(s) 541
PaeI GCATGC 1 cut(s) 71
PaeR7I CTCGAG 1 cut(s) 435
PagI TCATGA 1 cut(s) 816
PciI ACATGT 1 cut(s) 253
PciSI GCTCTTC 1 cut(s) 921
PctI GAATGC 2 cut(s) 856, 865
PflFI GACNNNGTC 1 cut(s) 297
PflMI CCANNNNNTGG 1 cut(s) 841
PscI ACATGT 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 295, 458
PspGI CCWGG 2 cut(s) 295, 458
PspPI GGNCC 1 cut(s) 299
PstI CTGCAG 4 cut(s) 178, 364, 382, 928
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 297
RsaI GTAC 3 cut(s) 699, 875, 897
RsaNI GTAC 3 cut(s) 698, 874, 896
RseI CAYNNNNRTG 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 5 cut(s) 143, 209, 483, 528, 585
Sau3AI GATC 4 cut(s) 4, 819, 845, 933
Sau96I GGNCC 1 cut(s) 299
ScrFI CCNGG 4 cut(s) 57, 58, 297, 460
SduI GDGCHC 2 cut(s) 42, 152
SexAI ACCWGGT 1 cut(s) 295
SfaNI GCATC 5 cut(s) 54, 591, 617, 625, 949
SfcI CTRYAG 5 cut(s) 174, 360, 378, 828, 924
Sfr274I CTCGAG 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 541
SinI GGWCC 1 cut(s) 299
SlaI CTCGAG 1 cut(s) 435
SmaI CCCGGG 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 72
SmlI CTYRAG 2 cut(s) 128, 435
SmoI CTYRAG 2 cut(s) 128, 435
SphI GCATGC 1 cut(s) 71
Sse9I AATT 6 cut(s) 23, 140, 231, 282, 387, 678
SsiI CCGC 2 cut(s) 498, 807
SspMI CTAG 3 cut(s) 291, 452, 988
StyD4I CCNGG 4 cut(s) 55, 56, 295, 458
StyI CCWWGG 2 cut(s) 723, 840
TaaI ACNGT 8 cut(s) 154, 337, 472, 488, 520, 634, 676, 873
TaqI TCGA 3 cut(s) 436, 541, 848
TaqII GACCGA 1 cut(s) 394
TasI AATT 6 cut(s) 23, 140, 231, 282, 387, 678
TatI WGTACW 1 cut(s) 697
TauI GCSGC 2 cut(s) 500, 809
Tru1I TTAA 5 cut(s) 143, 209, 483, 528, 585
Tru9I TTAA 5 cut(s) 143, 209, 483, 528, 585
TscAI CASTG 3 cut(s) 618, 626, 639
TseFI GTSAC 1 cut(s) 331
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 6 cut(s) 77, 631, 663, 699, 805, 908
TspMI CCCGGG 1 cut(s) 56
TspRI CASTG 3 cut(s) 618, 626, 639
Tth111I GACNNNGTC 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 841
VpaK11BI GGWCC 1 cut(s) 299
XapI RAATTY 1 cut(s) 231
XbaI TCTAGA 2 cut(s) 290, 451
XceI RCATGY 3 cut(s) 71, 257, 359
XhoI CTCGAG 1 cut(s) 435
XmaI CCCGGG 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 562
XspI CTAG 3 cut(s) 291, 452, 988
Zsp2I ATGCAT 2 cut(s) 69, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.