Prupe.7G099800_v2.0.a1

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0015657
Physical Location & Seq
Forward (+)
13045755 .. 13048447
2693 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G099800.1

Sequence Viewer

Length: 1326 bp
ATGGAGTGGCTGCGTCTGTTGTTGTTGCTATCAGCGTTAGCAATAACAAATCATGTGTCGTGTTCATGGGGTTTGGAGGCTGGTCCGAGTTGGGAGCTGAGTCAACTGAATCGGGGGCTGCTGGACTCGGCCAGGCAGCCCGAGTTCTTTGATTGGTTGAAAAGGGTTAGGAGGAGGATCCACGAAAATCCAGAGCTGGCCTTCGAGGAGCACGAGACGAGTCAACTCATTAGATCGGAACTCGACTCGCTCGGGATCCAGTACACGTGGCCGGTGGCCAAGACTGGGTTGGTGGCTTCTATTGGTTCTGGGTCGCAGCCATGGTTCGCTCTGAGGGCTGACATGGATGCTCTCCCAATTCAGGAATTGGTTGAGTGGGAGCACAAGAGCAAGAACCCTGGAAAGATGCATGCTTGTGGGCATGATGCTCATGTAACAATGCTGCTTGGAGCAGCTAAGTTACTTCAGAGCAAAAGCAAAGAAATAAAGGGCACAATCAAGCTTGTTTTTCAACCTGCAGAAGAGGGTCATGGAGGGGCTTACCATATGATAAAAGAGGGTGCTCTTGATAACATTCAAGGCATTTTTGGGTTACATATTCAACCTCTAATGCCCGTTGGTACTATTGGTTCCAGGCCAGGTCCAATTCTTGCAGGCTCTGGTAGATTTACAGTCACAATTCATGGGAAAGGCGGGCATGCTGCGTTTCCCCATTTGGCCACAGATCCAATTCTTGCTGCCTGCTCGGCCGTCATTGCCCTTCAACAGCTTATTTCTAGAGAAACAGACCCTCTAGAGGCCAGGGTGGTCACTGTTGGGATGGTTGAGGGTGGCCAAGCAGGAAATGTGATCCCAGAGACAGTGAGACTTGGAGGCACTTTTCGAAGCATGTCTTCAGAAGGTCTATACTACCTTCAGCAAAGGATTCAAGAGGTCATAGAGATGCAAGCTTCGGTGCACCGGTGCATGGCAACGGTAGACTTCATGCTGGAAAAAATGAGGCCCTATCCAGCCACAGTTAACGATGAAGCAATGTACAAACATGCAAAGAATGTTGGAGAAACTTTGCTTGGGGAACCAAATGTGAAGCTTCTCCCAATGTCTATGGGAGCAGAGGACTTCAGCTTTTACACAGAAAAAATGGCAGCTGCATTCTTCATGATTGGGACAAAGAACGAGACTTTGGACCCAAAAAGACTTTGGCATTCACCCTATTTGGTAATTGATGAGGAGGTTCTTCCAATAGGAGCTGCCCTTCATGCTGCTGTTGCAATTTCTTACTTGGATAATATTGATGCTGCTGTAACTCATAATCTATTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

442

Amino Acids

48.51

Weight (kDa)

5.91

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccI GTMKAC 1 cut(s) 978
AciI CCGC 1 cut(s) 693
AclWI GGATC 6 cut(s) 172, 185, 250, 263, 719, 844
AcoI YGGCCR 6 cut(s) 129, 269, 276, 717, 747, 832
AcuI CTGAAG 4 cut(s) 449, 879, 899, 1105
AcvI CACGTG 1 cut(s) 267
AfaI GTAC 3 cut(s) 263, 622, 1037
AfiI CCNNNNNNNGG 4 cut(s) 285, 361, 796, 967
AflIII ACRYGT 1 cut(s) 264
AgeI ACCGGT 1 cut(s) 960
AgsI TTSAA 6 cut(s) 160, 512, 578, 602, 764, 929
AjnI CCWGG 5 cut(s) 131, 397, 632, 637, 800
AjuI GAANNNNNNNTTGG 4 cut(s) 1053, 1085, 1166, 1198
Alw21I GWGCWC 4 cut(s) 213, 384, 565, 960
Alw26I GTCTC 4 cut(s) 209, 851, 859, 1172
Alw44I GTGCAC 1 cut(s) 956
AlwI GGATC 6 cut(s) 172, 185, 250, 263, 719, 844
AlwNI CAGNNNCTG 1 cut(s) 659
Ama87I CYCGRG 2 cut(s) 140, 251
ApaLI GTGCAC 1 cut(s) 956
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AsiGI ACCGGT 1 cut(s) 960
AspS9I GGNCC 4 cut(s) 83, 641, 1002, 1186
AsuHPI GGTGA 1 cut(s) 1200
AsuII TTCGAA 1 cut(s) 883
AvaI CYCGRG 2 cut(s) 140, 251
AvaII GGWCC 3 cut(s) 83, 641, 1186
BaeGI GKGCMC 2 cut(s) 494, 960
BalI TGGCCA 3 cut(s) 278, 719, 834
BamHI GGATCC 2 cut(s) 177, 255
BauI CACGAG 1 cut(s) 212
BbrPI CACGTG 1 cut(s) 267
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 4 cut(s) 213, 384, 565, 960
BccI CCATC 1 cut(s) 814
BceAI ACGGC 1 cut(s) 734
BciT130I CCWGG 5 cut(s) 133, 399, 634, 639, 802
BcoDI GTCTC 4 cut(s) 209, 851, 859, 1172
BfaI CTAG 2 cut(s) 777, 794
BfmI CTRYAG 1 cut(s) 516
BfuAI ACCTGC 1 cut(s) 523
BglI GCCNNNNNGGC 1 cut(s) 746
Bme1390I CCNGG 5 cut(s) 133, 399, 634, 639, 802
Bme18I GGWCC 3 cut(s) 83, 641, 1186
BmeT110I CYCGRG 2 cut(s) 140, 251
BmgT120I GGNCC 4 cut(s) 83, 641, 1002, 1186
BmiI GGNNCC 5 cut(s) 179, 257, 631, 1077, 1188
BmrFI CCNGG 5 cut(s) 133, 399, 634, 639, 802
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 5 cut(s) 337, 396, 415, 933, 1285
BmuI ACTGGG 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 885
Bpu14I TTCGAA 1 cut(s) 883
BsaAI YACGTR 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 320, 397, 801
BsaWI WCCGGW 1 cut(s) 960
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 361, 796, 967
Bse118I RCCGGY 2 cut(s) 271, 960
Bse1I ACTGG 2 cut(s) 259, 289
Bse3DI GCAATG 2 cut(s) 753, 1038
BseBI CCWGG 5 cut(s) 133, 399, 634, 639, 802
BseDI CCNNGG 3 cut(s) 320, 397, 801
BseGI GGATG 2 cut(s) 352, 825
BseLI CCNNNNNNNGG 4 cut(s) 285, 361, 796, 967
BseMI GCAATG 2 cut(s) 753, 1038
BseMII CTCAG 2 cut(s) 89, 323
BseNI ACTGG 2 cut(s) 259, 289
BseRI GAGGAG 3 cut(s) 187, 221, 1244
BseSI GKGCMC 2 cut(s) 494, 960
BseX3I CGGCCG 1 cut(s) 747
Bsh1285I CGRYCG 1 cut(s) 750
BshTI ACCGGT 1 cut(s) 960
BsiEI CGRYCG 1 cut(s) 750
BsiHKAI GWGCWC 4 cut(s) 213, 384, 565, 960
BsiHKCI CYCGRG 2 cut(s) 140, 251
BsiSI CCGG 2 cut(s) 272, 961
BslFI GGGAC 1 cut(s) 1180
BslI CCNNNNNNNGG 4 cut(s) 285, 361, 796, 967
BsmAI GTCTC 4 cut(s) 209, 851, 859, 1172
BsmBI CGTCTC 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 1180
BsmI GAATGC 2 cut(s) 1151, 1204
BsoBI CYCGRG 2 cut(s) 140, 251
Bsp119I TTCGAA 1 cut(s) 883
Bsp1286I GDGCHC 5 cut(s) 213, 384, 494, 565, 960
Bsp1407I TGTACA 1 cut(s) 1035
Bsp143I GATC 5 cut(s) 177, 233, 255, 724, 849
Bsp19I CCATGG 1 cut(s) 320
BspACI CCGC 1 cut(s) 693
BspCNI CTCAG 2 cut(s) 90, 324
BspHI TCATGA 1 cut(s) 1158
BspLI GGNNCC 5 cut(s) 179, 257, 631, 1077, 1188
BspMAI CTGCAG 1 cut(s) 520
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 6 cut(s) 172, 185, 250, 263, 719, 844
BspT104I TTCGAA 1 cut(s) 883
BsrDI GCAATG 2 cut(s) 753, 1038
BsrFI RCCGGY 2 cut(s) 271, 960
BsrGI TGTACA 1 cut(s) 1035
BsrI ACTGG 2 cut(s) 259, 289
BssAI RCCGGY 2 cut(s) 271, 960
BssECI CCNNGG 3 cut(s) 320, 397, 801
BssMI GATC 5 cut(s) 177, 233, 255, 724, 849
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 320
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 5 cut(s) 133, 399, 634, 639, 802
Bst4CI ACNGT 5 cut(s) 673, 814, 862, 976, 1018
Bst6I CTCTTC 1 cut(s) 516
BstAUI TGTACA 1 cut(s) 1035
BstBAI YACGTR 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 883
BstC8I GCNNGC 7 cut(s) 198, 411, 655, 695, 699, 742, 948
BstDEI CTNAG 3 cut(s) 98, 332, 456
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 2 cut(s) 352, 825
BstKTI GATC 5 cut(s) 180, 236, 258, 727, 852
BstMAI GTCTC 4 cut(s) 209, 851, 859, 1172
BstMBI GATC 5 cut(s) 177, 233, 255, 724, 849
BstMCI CGRYCG 1 cut(s) 750
BstMWI GCNNNNNNNGC 5 cut(s) 335, 746, 755, 1259, 1268
BstNI CCWGG 5 cut(s) 133, 399, 634, 639, 802
BstNSI RCATGY 4 cut(s) 413, 701, 892, 1046
BstSCI CCNGG 5 cut(s) 131, 397, 632, 637, 800
BstSFI CTRYAG 1 cut(s) 516
BstSLI GKGCMC 2 cut(s) 494, 960
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 3 cut(s) 177, 255, 724
BstYI RGATCY 3 cut(s) 177, 255, 724
BstZI CGGCCG 1 cut(s) 747
BtgI CCRYGG 1 cut(s) 320
BtsCI GGATG 2 cut(s) 352, 825
BtsIMutI CAGTG 2 cut(s) 810, 867
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 7 cut(s) 198, 411, 655, 695, 699, 742, 948
CaiI CAGNNNCTG 1 cut(s) 659
CciI TCATGA 1 cut(s) 1158
Cfr10I RCCGGY 2 cut(s) 271, 960
Cfr13I GGNCC 4 cut(s) 83, 641, 1002, 1186
CseI GACGC 1 cut(s) 2
Csp6I GTAC 3 cut(s) 262, 621, 1036
CspAI ACCGGT 1 cut(s) 960
CviQI GTAC 3 cut(s) 262, 621, 1036
DdeI CTNAG 3 cut(s) 98, 332, 456
DpnI GATC 5 cut(s) 179, 235, 257, 726, 851
DpnII GATC 5 cut(s) 177, 233, 255, 724, 849
EaeI YGGCCR 6 cut(s) 129, 269, 276, 717, 747, 832
EagI CGGCCG 1 cut(s) 747
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
EclXI CGGCCG 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 320
Eco47I GGWCC 3 cut(s) 83, 641, 1186
Eco52I CGGCCG 1 cut(s) 747
Eco57I CTGAAG 4 cut(s) 449, 879, 899, 1105
Eco72I CACGTG 1 cut(s) 267
Eco88I CYCGRG 2 cut(s) 140, 251
EcoO109I RGGNCCY 1 cut(s) 1002
EcoRII CCWGG 5 cut(s) 131, 397, 632, 637, 800
EcoT14I CCWWGG 1 cut(s) 320
EcoT22I ATGCAT 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 320
Esp3I CGTCTC 1 cut(s) 209
FalI AAGNNNNNCTT 2 cut(s) 877, 909
FaqI GGGAC 1 cut(s) 1180
FauI CCCGC 1 cut(s) 686
FauNDI CATATG 1 cut(s) 546
FblI GTMKAC 1 cut(s) 978
FokI GGATG 2 cut(s) 359, 832
FspBI CTAG 2 cut(s) 777, 794
HapII CCGG 2 cut(s) 272, 961
HgaI GACGC 1 cut(s) 2
HincII GTYRAC 3 cut(s) 104, 224, 1021
HindII GTYRAC 3 cut(s) 104, 224, 1021
HindIII AAGCTT 3 cut(s) 500, 948, 1088
HinfI GANTC 6 cut(s) 100, 109, 125, 220, 245, 925
HpaI GTTAAC 1 cut(s) 1021
HpaII CCGG 2 cut(s) 272, 961
HphI GGTGA 1 cut(s) 1200
Hpy166II GTNNAC 6 cut(s) 104, 224, 264, 958, 979, 1021
Hpy188I TCNGA 5 cut(s) 87, 238, 333, 468, 898
Hpy188III TCNNGA 8 cut(s) 191, 253, 362, 566, 777, 794, 929, 1159
Hpy8I GTNNAC 6 cut(s) 104, 224, 264, 958, 979, 1021
HpyAV CCTTC 5 cut(s) 211, 770, 893, 923, 1265
HpyCH4III ACNGT 5 cut(s) 673, 814, 862, 976, 1018
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 9 cut(s) 409, 518, 653, 946, 958, 966, 1046, 1151, 1271
HpyF10VI GCNNNNNNNGC 5 cut(s) 335, 746, 755, 1259, 1268
HpyF3I CTNAG 3 cut(s) 98, 332, 456
HpySE526I ACGT 1 cut(s) 266
KspAI GTTAAC 1 cut(s) 1021
Kzo9I GATC 5 cut(s) 177, 233, 255, 724, 849
LmnI GCTCC 6 cut(s) 94, 208, 379, 449, 1109, 1247
LweI GCATC 5 cut(s) 337, 396, 415, 933, 1285
MaeI CTAG 2 cut(s) 777, 794
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 6 cut(s) 433, 459, 591, 673, 808, 1303
MalI GATC 5 cut(s) 179, 235, 257, 726, 851
MboI GATC 5 cut(s) 177, 233, 255, 724, 849
MboII GAAGA 4 cut(s) 533, 885, 1147, 1229
MflI RGATCY 3 cut(s) 177, 255, 724
MhlI GDGCHC 5 cut(s) 213, 384, 494, 565, 960
MlsI TGGCCA 3 cut(s) 278, 719, 834
MluCI AATT 7 cut(s) 357, 365, 645, 678, 729, 1221, 1272
MluNI TGGCCA 3 cut(s) 278, 719, 834
MlyI GAGTC 4 cut(s) 109, 119, 229, 239
MmeI TCCRAC 1 cut(s) 1036
Mox20I TGGCCA 3 cut(s) 278, 719, 834
Mph1103I ATGCAT 1 cut(s) 411
MscI TGGCCA 3 cut(s) 278, 719, 834
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 2 cut(s) 414, 941
Msp20I TGGCCA 3 cut(s) 278, 719, 834
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 2 cut(s) 272, 961
MspR9I CCNGG 5 cut(s) 133, 399, 634, 639, 802
Mva1269I GAATGC 2 cut(s) 1151, 1204
MvaI CCWGG 5 cut(s) 133, 399, 634, 639, 802
MwoI GCNNNNNNNGC 5 cut(s) 335, 746, 755, 1259, 1268
NcoI CCATGG 1 cut(s) 320
NdeI CATATG 1 cut(s) 546
NdeII GATC 5 cut(s) 177, 233, 255, 724, 849
NlaIV GGNNCC 5 cut(s) 179, 257, 631, 1077, 1188
NmeAIII GCCGAG 2 cut(s) 107, 725
NmuCI GTSAC 2 cut(s) 673, 808
NsiI ATGCAT 1 cut(s) 411
NspI RCATGY 4 cut(s) 413, 701, 892, 1046
NspV TTCGAA 1 cut(s) 883
PaeI GCATGC 2 cut(s) 413, 701
PagI TCATGA 1 cut(s) 1158
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 2 cut(s) 1151, 1204
PfeI GAWTC 2 cut(s) 109, 925
PinAI ACCGGT 1 cut(s) 960
PleI GAGTC 4 cut(s) 108, 119, 228, 239
PmaCI CACGTG 1 cut(s) 267
PmlI CACGTG 1 cut(s) 267
PpsI GAGTC 4 cut(s) 108, 119, 228, 239
Ppu21I YACGTR 1 cut(s) 267
Psp6I CCWGG 5 cut(s) 131, 397, 632, 637, 800
PspCI CACGTG 1 cut(s) 267
PspGI CCWGG 5 cut(s) 131, 397, 632, 637, 800
PspN4I GGNNCC 5 cut(s) 179, 257, 631, 1077, 1188
PspPI GGNCC 4 cut(s) 83, 641, 1002, 1186
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PstI CTGCAG 1 cut(s) 520
PstNI CAGNNNCTG 1 cut(s) 659
PsuI RGATCY 3 cut(s) 177, 255, 724
PvuII CAGCTG 1 cut(s) 1148
RsaI GTAC 3 cut(s) 263, 622, 1037
RsaNI GTAC 3 cut(s) 262, 621, 1036
RseI CAYNNNNRTG 2 cut(s) 414, 941
SaqAI TTAA 1 cut(s) 1020
Sau3AI GATC 5 cut(s) 177, 233, 255, 724, 849
Sau96I GGNCC 4 cut(s) 83, 641, 1002, 1186
SchI GAGTC 4 cut(s) 109, 119, 229, 239
ScrFI CCNGG 5 cut(s) 133, 399, 634, 639, 802
SduI GDGCHC 5 cut(s) 213, 384, 494, 565, 960
SfaNI GCATC 5 cut(s) 337, 396, 415, 933, 1285
SfcI CTRYAG 1 cut(s) 516
SfuI TTCGAA 1 cut(s) 883
SgrAI CRCCGGYG 1 cut(s) 960
SinI GGWCC 3 cut(s) 83, 641, 1186
SmiMI CAYNNNNRTG 2 cut(s) 414, 941
SphI GCATGC 2 cut(s) 413, 701
Sse9I AATT 7 cut(s) 357, 365, 645, 678, 729, 1221, 1272
SsiI CCGC 1 cut(s) 693
SspI AATATT 1 cut(s) 1291
SspMI CTAG 2 cut(s) 777, 794
StyD4I CCNGG 5 cut(s) 131, 397, 632, 637, 800
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 5 cut(s) 673, 814, 862, 976, 1018
TaiI ACGT 1 cut(s) 269
TaqI TCGA 3 cut(s) 204, 243, 883
TasI AATT 7 cut(s) 357, 365, 645, 678, 729, 1221, 1272
TatI WGTACW 2 cut(s) 261, 1035
TfiI GAWTC 2 cut(s) 109, 925
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 2 cut(s) 817, 867
TseFI GTSAC 2 cut(s) 673, 808
Tsp45I GTSAC 2 cut(s) 673, 808
TspDTI ATGAA 6 cut(s) 54, 671, 973, 1041, 1147, 1247
TspRI CASTG 2 cut(s) 817, 867
VneI GTGCAC 1 cut(s) 956
VpaK11BI GGWCC 3 cut(s) 83, 641, 1186
XbaI TCTAGA 2 cut(s) 776, 793
XceI RCATGY 4 cut(s) 413, 701, 892, 1046
XcmI CCANNNNNNNNNTGG 2 cut(s) 286, 1197
XmiI GTMKAC 1 cut(s) 978
XspI CTAG 2 cut(s) 777, 794
Zsp2I ATGCAT 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.