Rw3G017850

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015657
Physical Location & Seq
Reverse (-)
19605658 .. 19607953
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G017850.1

Sequence Viewer

Length: 1077 bp
ATGCTCTCCCAATTCAGGTCAGTCATTCTCTCTCTTCCGGAAGTTCAGTTTAAACAAGTACAAGAAATGAAGTTTCCCAAAATGAATTCATGTAAGGAATTGGTTGAGTGGGAGCACAAGAGCAAGAACCCCGGGAAGATGCATGCTTGTGGCCATGATGTTCATGTAACCATGCTGCTTGGGGCTGCTAAGTTACTTCAGCTCAAGAGTAAACAATTAAAGGGCACAGTCAAGCTTGTTTTTCAACCTGCAGAGGAGGGTCATGCAGGGGCATACCATATGTTAAAAGAGGATGCTCTTGATGGAATTCAAGGCATTTTTGGATTACATGTTTCGCCAGAAATGCAGACTGGTTCAATTGGTTCTAGACCTGGTCCATTTCTTGCTGGTGCTGGTAGATTTTTAGTCACAGTTCAAGGAAAAGGTGGACATGCTGCAGCTCCCCATTTGACTGCAGACCCAATTCTTGCAGCCTGCTCGGTCATCCTTGCGCTCCAACAGATTGTATCTCGAGAAACCGACCCTCTAGAAGCCAGGGTGGTAACAGTTGGGTTTGTTAACGGTGGCCAAGCCGCAAATGTCATTCCAGAGACGGTGGAACTTAGAGGGACTTTTCGAAGCTTGACTTTAGATGGTATACATTACCTTCAGCAGAGGATTAAGGAGGTGATAGAGATGCAAGCATCAGTGCATCAGTGTAATGCAACAGTGGACTTCATGCATGAAAAAATGAGGCCGTATCCACCAACTGTAATTGATGAAGCACTCTATGTACACAGCAAGAGTGTTAGTGAAGCCTTGGTTGGGGAACCAAATGTGTATCTTCTTCCAATGACTATGGCAGCAGAGGACTTTAGCTTCTATACACATAAAATGCCAGCCGCAATGTTCATGATCGGGACTACAGACCATACCTTGGGATCGAATGCTACAAGTTTGCATTCACCGTACTTGGTCATTGATGAGGAAGTACTTCCAATAGGAGCGGCTCTTCATGCTGCAGTTGCGATCTCGTTCTTGGATAACAATACTGATGCTGCTGCTGCTGCTGCTGCTGCGCACTCAGTCATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

358

Amino Acids

38.68

Weight (kDa)

6.16

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 36 - 336 2.6e-35 Peptidase family M20/M25/M40
M20_dimer PF07687 130 - 226 5.9e-13 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 256
AccB7I CCANNNNNTGG 1 cut(s) 916
AccBSI CCGCTC 1 cut(s) 986
AccI GTMKAC 1 cut(s) 637
AccIII TCCGGA 1 cut(s) 37
AciI CCGC 3 cut(s) 573, 882, 986
AclWI GGATC 1 cut(s) 928
AcoI YGGCCR 2 cut(s) 151, 565
AcsI RAATTY 2 cut(s) 85, 306
AcuI CTGAAG 2 cut(s) 182, 632
AfaI GTAC 4 cut(s) 60, 774, 950, 972
AfiI CCNNNNNNNGG 3 cut(s) 15, 804, 916
AflIII ACRYGT 1 cut(s) 328
AgsI TTSAA 4 cut(s) 245, 311, 357, 416
AjnI CCWGG 2 cut(s) 370, 533
AjuI GAANNNNNNNTTGG 2 cut(s) 786, 818
AluBI AGCT 5 cut(s) 202, 235, 440, 621, 858
AluI AGCT 5 cut(s) 202, 235, 440, 621, 858
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 1 cut(s) 928
Ama87I CYCGRG 2 cut(s) 131, 510
Aor13HI TCCGGA 1 cut(s) 37
AoxI GGCC 3 cut(s) 151, 565, 734
ApoI RAATTY 2 cut(s) 85, 306
Asp700I GAANNNNTTC 1 cut(s) 972
AspLEI GCGC 2 cut(s) 493, 1060
AspS9I GGNCC 1 cut(s) 374
AsuC2I CCSGG 2 cut(s) 132, 133
AsuHPI GGTGA 2 cut(s) 679, 936
AsuII TTCGAA 1 cut(s) 616
AvaI CYCGRG 2 cut(s) 131, 510
AvaII GGWCC 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 227
BalI TGGCCA 2 cut(s) 153, 567
Bbv12I GWGCWC 1 cut(s) 117
BccI CCATC 2 cut(s) 296, 626
BceAI ACGGC 1 cut(s) 721
BcgI CGANNNNNNTGC 2 cut(s) 459, 493
BciT130I CCWGG 2 cut(s) 372, 535
BciVI GTATCC 1 cut(s) 750
BcnI CCSGG 2 cut(s) 132, 133
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 3 cut(s) 366, 527, 1075
BfmI CTRYAG 5 cut(s) 249, 435, 453, 903, 999
BfuAI ACCTGC 1 cut(s) 256
BfuI GTATCC 1 cut(s) 750
BmcAI AGTACT 1 cut(s) 972
Bme1390I CCNGG 4 cut(s) 132, 133, 372, 535
Bme18I GGWCC 1 cut(s) 374
BmeT110I CYCGRG 2 cut(s) 131, 510
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 4 cut(s) 132, 133, 372, 535
BmsI GCATC 6 cut(s) 129, 283, 666, 692, 700, 1024
Bpu14I TTCGAA 1 cut(s) 616
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 2 cut(s) 132, 133
BsaJI CCNNGG 5 cut(s) 130, 131, 534, 798, 915
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 804, 916
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 891
BseAI TCCGGA 1 cut(s) 37
BseBI CCWGG 2 cut(s) 372, 535
BseDI CCNNGG 5 cut(s) 130, 131, 534, 798, 915
BseGI GGATG 2 cut(s) 298, 483
BseLI CCNNNNNNNGG 3 cut(s) 15, 804, 916
BseMI GCAATG 1 cut(s) 891
BseMII CTCAG 1 cut(s) 1077
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 269
BseSI GKGCMC 1 cut(s) 227
BshFI GGCC 3 cut(s) 153, 567, 736
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 2 cut(s) 131, 510
BsiSI CCGG 2 cut(s) 38, 132
BslFI GGGAC 2 cut(s) 622, 913
BslI CCNNNNNNNGG 3 cut(s) 15, 804, 916
BsmAI GTCTC 1 cut(s) 584
BsmBI CGTCTC 1 cut(s) 584
BsmFI GGGAC 2 cut(s) 622, 913
BsmI GAATGC 2 cut(s) 931, 940
BsnI GGCC 3 cut(s) 153, 567, 736
BsoBI CYCGRG 2 cut(s) 131, 510
Bsp119I TTCGAA 1 cut(s) 616
Bsp1286I GDGCHC 2 cut(s) 117, 227
Bsp13I TCCGGA 1 cut(s) 37
Bsp1407I TGTACA 1 cut(s) 772
Bsp143I GATC 3 cut(s) 894, 920, 1008
BspACI CCGC 3 cut(s) 573, 882, 986
BspANI GGCC 3 cut(s) 153, 567, 736
BspCNI CTCAG 1 cut(s) 1076
BspEI TCCGGA 1 cut(s) 37
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 1 cut(s) 810
BspMAI CTGCAG 4 cut(s) 253, 439, 457, 1003
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 1 cut(s) 928
BspQI GCTCTTC 1 cut(s) 996
BspT104I TTCGAA 1 cut(s) 616
BsrBI CCGCTC 1 cut(s) 986
BsrDI GCAATG 1 cut(s) 891
BsrGI TGTACA 1 cut(s) 772
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 5 cut(s) 130, 131, 534, 798, 915
BssMI GATC 3 cut(s) 894, 920, 1008
BssNAI GTATAC 1 cut(s) 638
BssT1I CCWWGG 2 cut(s) 798, 915
Bst1107I GTATAC 1 cut(s) 638
Bst2UI CCWGG 2 cut(s) 372, 535
Bst4CI ACNGT 8 cut(s) 229, 412, 547, 563, 595, 709, 751, 948
Bst6I CTCTTC 2 cut(s) 39, 996
BstAUI TGTACA 1 cut(s) 772
BstBI TTCGAA 1 cut(s) 616
BstC8I GCNNGC 4 cut(s) 144, 475, 681, 879
BstDEI CTNAG 3 cut(s) 189, 602, 1063
BstF5I GGATG 2 cut(s) 298, 483
BstHHI GCGC 2 cut(s) 493, 1060
BstKTI GATC 3 cut(s) 897, 923, 1011
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 3 cut(s) 894, 920, 1008
BstMWI GCNNNNNNNGC 8 cut(s) 343, 995, 1004, 1043, 1046, 1049, 1052, 1055
BstNI CCWGG 2 cut(s) 372, 535
BstNSI RCATGY 3 cut(s) 146, 332, 434
BstSCI CCNGG 4 cut(s) 130, 131, 370, 533
BstSFI CTRYAG 5 cut(s) 249, 435, 453, 903, 999
BstSLI GKGCMC 1 cut(s) 227
BstZ17I GTATAC 1 cut(s) 638
BsuI GTATCC 1 cut(s) 750
BsuRI GGCC 3 cut(s) 153, 567, 736
BtsCI GGATG 2 cut(s) 298, 483
BtsIMutI CAGTG 3 cut(s) 693, 701, 714
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 144, 475, 681, 879
CciI TCATGA 1 cut(s) 891
CfoI GCGC 2 cut(s) 493, 1060
Cfr13I GGNCC 1 cut(s) 374
Cfr9I CCCGGG 1 cut(s) 131
CsiI ACCWGGT 1 cut(s) 370
Csp6I GTAC 4 cut(s) 59, 773, 949, 971
CviQI GTAC 4 cut(s) 59, 773, 949, 971
DdeI CTNAG 3 cut(s) 189, 602, 1063
DpnI GATC 3 cut(s) 896, 922, 1010
DpnII GATC 3 cut(s) 894, 920, 1008
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 2 cut(s) 151, 565
Eam1104I CTCTTC 2 cut(s) 39, 996
EarI CTCTTC 2 cut(s) 39, 996
Eco130I CCWWGG 2 cut(s) 798, 915
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 2 cut(s) 182, 632
Eco88I CYCGRG 2 cut(s) 131, 510
EcoRI GAATTC 2 cut(s) 85, 306
EcoRII CCWGG 2 cut(s) 370, 533
EcoT14I CCWWGG 2 cut(s) 798, 915
EcoT22I ATGCAT 2 cut(s) 144, 723
ErhI CCWWGG 2 cut(s) 798, 915
Esp3I CGTCTC 1 cut(s) 584
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FaqI GGGAC 2 cut(s) 622, 913
FauNDI CATATG 1 cut(s) 279
FblI GTMKAC 1 cut(s) 637
FokI GGATG 2 cut(s) 305, 470
FspBI CTAG 3 cut(s) 366, 527, 1075
FspI TGCGCA 1 cut(s) 1059
GlaI GCGC 2 cut(s) 492, 1059
HaeIII GGCC 3 cut(s) 153, 567, 736
HapII CCGG 2 cut(s) 38, 132
HhaI GCGC 2 cut(s) 493, 1060
Hin6I GCGC 2 cut(s) 491, 1058
HinP1I GCGC 2 cut(s) 491, 1058
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HindIII AAGCTT 2 cut(s) 233, 619
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 2 cut(s) 38, 132
HphI GGTGA 2 cut(s) 679, 936
Hpy166II GTNNAC 6 cut(s) 212, 428, 559, 638, 712, 775
Hpy8I GTNNAC 6 cut(s) 212, 428, 559, 638, 712, 775
HpyAV CCTTC 1 cut(s) 656
HpyCH4III ACNGT 8 cut(s) 229, 412, 547, 563, 595, 709, 751, 948
HpyF10VI GCNNNNNNNGC 8 cut(s) 343, 995, 1004, 1043, 1046, 1049, 1052, 1055
HpyF3I CTNAG 3 cut(s) 189, 602, 1063
HspAI GCGC 2 cut(s) 491, 1058
Kpn2I TCCGGA 1 cut(s) 37
KspAI GTTAAC 1 cut(s) 559
Kzo9I GATC 3 cut(s) 894, 920, 1008
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 4 cut(s) 112, 445, 498, 983
LweI GCATC 6 cut(s) 129, 283, 666, 692, 700, 1024
MabI ACCWGGT 1 cut(s) 370
MaeI CTAG 3 cut(s) 366, 527, 1075
MaeIII GTNAC 4 cut(s) 166, 192, 406, 541
MalI GATC 3 cut(s) 896, 922, 1010
MbiI CCGCTC 1 cut(s) 986
MboI GATC 3 cut(s) 894, 920, 1008
MboII GAAGA 5 cut(s) 26, 148, 815, 818, 983
MfeI CAATTG 1 cut(s) 357
MhlI GDGCHC 2 cut(s) 117, 227
MlsI TGGCCA 2 cut(s) 153, 567
MluCI AATT 8 cut(s) 11, 85, 98, 215, 306, 357, 462, 753
MluNI TGGCCA 2 cut(s) 153, 567
MmeI TCCRAC 1 cut(s) 520
Mox20I TGGCCA 2 cut(s) 153, 567
Mph1103I ATGCAT 2 cut(s) 144, 723
MroI TCCGGA 1 cut(s) 37
MroXI GAANNNNTTC 1 cut(s) 972
MscI TGGCCA 2 cut(s) 153, 567
MseI TTAA 5 cut(s) 51, 218, 284, 558, 660
MslI CAYNNNNRTG 1 cut(s) 147
Msp20I TGGCCA 2 cut(s) 153, 567
MspI CCGG 2 cut(s) 38, 132
MspR9I CCNGG 4 cut(s) 132, 133, 372, 535
MssI GTTTAAAC 1 cut(s) 52
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 2 cut(s) 931, 940
MvaI CCWGG 2 cut(s) 372, 535
MwoI GCNNNNNNNGC 8 cut(s) 343, 995, 1004, 1043, 1046, 1049, 1052, 1055
NciI CCSGG 2 cut(s) 132, 133
NdeI CATATG 1 cut(s) 279
NdeII GATC 3 cut(s) 894, 920, 1008
NlaIV GGNNCC 1 cut(s) 810
NmuCI GTSAC 1 cut(s) 406
NsbI TGCGCA 1 cut(s) 1059
NsiI ATGCAT 2 cut(s) 144, 723
NspI RCATGY 3 cut(s) 146, 332, 434
NspV TTCGAA 1 cut(s) 616
PaeI GCATGC 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 510
PagI TCATGA 1 cut(s) 891
PciI ACATGT 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 2 cut(s) 931, 940
PdmI GAANNNNTTC 1 cut(s) 972
PflFI GACNNNGTC 1 cut(s) 372
PflMI CCANNNNNTGG 1 cut(s) 916
PmeI GTTTAAAC 1 cut(s) 52
PscI ACATGT 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 370, 533
PspGI CCWGG 2 cut(s) 370, 533
PspN4I GGNNCC 1 cut(s) 810
PspPI GGNCC 1 cut(s) 374
PstI CTGCAG 4 cut(s) 253, 439, 457, 1003
PsyI GACNNNGTC 1 cut(s) 372
RsaI GTAC 4 cut(s) 60, 774, 950, 972
RsaNI GTAC 4 cut(s) 59, 773, 949, 971
RseI CAYNNNNRTG 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 5 cut(s) 51, 218, 284, 558, 660
Sau3AI GATC 3 cut(s) 894, 920, 1008
Sau96I GGNCC 1 cut(s) 374
ScaI AGTACT 1 cut(s) 972
ScrFI CCNGG 4 cut(s) 132, 133, 372, 535
SduI GDGCHC 2 cut(s) 117, 227
SexAI ACCWGGT 1 cut(s) 370
SfaNI GCATC 6 cut(s) 129, 283, 666, 692, 700, 1024
SfcI CTRYAG 5 cut(s) 249, 435, 453, 903, 999
Sfr274I CTCGAG 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 616
SinI GGWCC 1 cut(s) 374
SlaI CTCGAG 1 cut(s) 510
SmaI CCCGGG 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 203, 510
SmoI CTYRAG 2 cut(s) 203, 510
SphI GCATGC 1 cut(s) 146
Sse9I AATT 8 cut(s) 11, 85, 98, 215, 306, 357, 462, 753
SsiI CCGC 3 cut(s) 573, 882, 986
SspMI CTAG 3 cut(s) 366, 527, 1075
StyD4I CCNGG 4 cut(s) 130, 131, 370, 533
StyI CCWWGG 2 cut(s) 798, 915
TaaI ACNGT 8 cut(s) 229, 412, 547, 563, 595, 709, 751, 948
TaqI TCGA 3 cut(s) 511, 616, 923
TaqII GACCGA 1 cut(s) 469
TasI AATT 8 cut(s) 11, 85, 98, 215, 306, 357, 462, 753
TatI WGTACW 3 cut(s) 58, 772, 970
TauI GCSGC 3 cut(s) 575, 884, 989
Tru1I TTAA 5 cut(s) 51, 218, 284, 558, 660
Tru9I TTAA 5 cut(s) 51, 218, 284, 558, 660
TscAI CASTG 3 cut(s) 693, 701, 714
TseFI GTSAC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 9 cut(s) 78, 83, 98, 152, 706, 738, 774, 880, 983
TspMI CCCGGG 1 cut(s) 131
TspRI CASTG 3 cut(s) 693, 701, 714
Tth111I GACNNNGTC 1 cut(s) 372
Van91I CCANNNNNTGG 1 cut(s) 916
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 2 cut(s) 85, 306
XbaI TCTAGA 2 cut(s) 365, 526
XceI RCATGY 3 cut(s) 146, 332, 434
XhoI CTCGAG 1 cut(s) 510
XmaI CCCGGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 637
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 3 cut(s) 366, 527, 1075
ZrmI AGTACT 1 cut(s) 972
Zsp2I ATGCAT 2 cut(s) 144, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.