Rh3DG221600

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0015657
Physical Location & Seq
Reverse (-)
20741862 .. 20743791
1930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG221600.1

Sequence Viewer

Length: 1002 bp
ATGGAGTGGCTGCGTATCGTGGTGTTATTTTCGACGTTAGCAATTCATGTATCATGGGGTCTAGAGAGCACTACTGGTTCGTTACCGGAGCTGAGTCAACTGAGTCGGGAGTTGCTGGACTCGGCCAGGGAGCCCGAGTTCTTTGATTGGTTGAGAAAAGTCAGGAGGAAAATCCACGAGAACCCTGAGCTCGCCTTTGAGGAGGACGAGACGAGCCAATTCATAAGATCGGAGCTCGACTTGCTTGGGATAGAGTACACGTGGCCGGTGGCCAAGACTGGAGTGGTTGCCTCCATCGGTTCTGGGTTGCAGCCATGGTTCGCTCTCAGGGCAGACATGGATGCTCTCCCAATTCAGGAATTGGTTGAGTGGGAGCACAAGAGCAAGAACCCCGGGAAGATGCATGCTTGTGGCCATGATGTTCATGTAACCATGCTGCTTGGAGCTGCTAAGTTACTTCAGCTCAAGAGTAAACAATTAAAGGGCACAGTCAAGCTTGTTTTTCAACCTGCAGAGGAGGGTCATGCAGGCGCATACCATATGTTAAAAGAGGTTGCTCTTGATGGAATTCAAGGCATTTTTGGATTACATGTTTCGCCAGAAATGCAGACTGGTTCAATTGGTTCTAGACCTGGTCCATTTCTTGCTGGTGCTGGTAGATTTTTAGTCACAGTTCAAGGAAAAGGTGGACATGCTGCAGCTCCCCATTTGACTGCAGACCCAATTCTTGCAGCCTGCTCGGTCATCCTTGCGCTCCAACAGATTGTATCTCGAGAAACCGACCCTCTAGAGGCCAGGGTGGTAACAGTTGGGTTTGTTAATGGTGGCCAAGCCGCAAATGTCATTCCAGAGACGGTGGAACTTAAAGGGACTTTTCGAAGCTTGACTTTCGATGGTATACATTACCTTCAGCAAAGGATTAAGGAGTATGACAATATAACATACCAGATATCTAGCAAATTATTATATTTCAAGCATGTATGGCTTGCATTCTACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

333

Amino Acids

36.95

Weight (kDa)

6.23

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 108 - 250 1.9e-29 Peptidase family M20/M25/M40
M20_dimer PF07687 217 - 310 3.2e-13 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 898
AciI CCGC 1 cut(s) 834
AcoI YGGCCR 5 cut(s) 123, 263, 270, 412, 826
AcsI RAATTY 1 cut(s) 567
AcuI CTGAAG 2 cut(s) 443, 893
AcvI CACGTG 1 cut(s) 261
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 355, 790
AflIII ACRYGT 2 cut(s) 258, 589
AgsI TTSAA 5 cut(s) 506, 572, 618, 677, 973
AjnI CCWGG 3 cut(s) 125, 631, 794
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 8 cut(s) 91, 190, 235, 446, 463, 496, 701, 882
AluI AGCT 8 cut(s) 91, 190, 235, 446, 463, 496, 701, 882
Alw21I GWGCWC 4 cut(s) 71, 192, 237, 378
Alw26I GTCTC 2 cut(s) 203, 845
Ama87I CYCGRG 3 cut(s) 134, 392, 771
AoxI GGCC 6 cut(s) 123, 263, 270, 412, 792, 826
ApeKI GCWGC 7 cut(s) 10, 310, 436, 446, 695, 698, 731
ApoI RAATTY 1 cut(s) 567
AspLEI GCGC 2 cut(s) 533, 754
AspS9I GGNCC 1 cut(s) 635
AsuC2I CCSGG 2 cut(s) 393, 394
AsuII TTCGAA 1 cut(s) 877
AvaI CYCGRG 3 cut(s) 134, 392, 771
AvaII GGWCC 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 488
BalI TGGCCA 3 cut(s) 272, 414, 828
BanII GRGCYC 3 cut(s) 135, 192, 237
BauI CACGAG 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 261
Bbv12I GWGCWC 4 cut(s) 71, 192, 237, 378
BbvI GCAGC 6 cut(s) 322, 423, 433, 682, 710, 743
BccI CCATC 3 cut(s) 302, 557, 887
BcgI CGANNNNNNTGC 2 cut(s) 720, 754
BciT130I CCWGG 3 cut(s) 127, 633, 796
BcnI CCSGG 2 cut(s) 393, 394
BcoDI GTCTC 2 cut(s) 203, 845
BfaI CTAG 4 cut(s) 62, 627, 788, 954
BfmI CTRYAG 4 cut(s) 510, 696, 714, 994
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 8 cut(s) 11, 311, 437, 447, 696, 699, 732, 834
BlsI GCNGC 8 cut(s) 12, 312, 438, 448, 697, 700, 733, 835
Bme1390I CCNGG 5 cut(s) 127, 393, 394, 633, 796
Bme18I GGWCC 1 cut(s) 635
BmeT110I CYCGRG 3 cut(s) 134, 392, 771
BmgT120I GGNCC 1 cut(s) 635
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 5 cut(s) 127, 393, 394, 633, 796
BmsI GCATC 2 cut(s) 331, 390
BpmI CTGGAG 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 186
Bpu14I TTCGAA 1 cut(s) 877
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 2 cut(s) 393, 394
BsaAI YACGTR 1 cut(s) 261
BsaJI CCNNGG 5 cut(s) 126, 314, 391, 392, 795
BsaWI WCCGGW 1 cut(s) 85
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 355, 790
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 3 cut(s) 79, 283, 616
BseBI CCWGG 3 cut(s) 127, 633, 796
BseDI CCNNGG 5 cut(s) 126, 314, 391, 392, 795
BseGI GGATG 2 cut(s) 346, 744
BseLI CCNNNNNNNGG 2 cut(s) 355, 790
BseMII CTCAG 4 cut(s) 83, 92, 177, 340
BseNI ACTGG 3 cut(s) 79, 283, 616
BseRI GAGGAG 2 cut(s) 215, 530
BseSI GKGCMC 1 cut(s) 488
BseXI GCAGC 6 cut(s) 322, 423, 433, 682, 710, 743
BshFI GGCC 6 cut(s) 125, 265, 272, 414, 794, 828
BsiHKAI GWGCWC 4 cut(s) 71, 192, 237, 378
BsiHKCI CYCGRG 3 cut(s) 134, 392, 771
BsiSI CCGG 3 cut(s) 86, 266, 393
BslFI GGGAC 1 cut(s) 883
BslI CCNNNNNNNGG 2 cut(s) 355, 790
BsmAI GTCTC 2 cut(s) 203, 845
BsmBI CGTCTC 2 cut(s) 203, 845
BsmFI GGGAC 1 cut(s) 883
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 6 cut(s) 125, 265, 272, 414, 794, 828
BsoBI CYCGRG 3 cut(s) 134, 392, 771
Bsp119I TTCGAA 1 cut(s) 877
Bsp1286I GDGCHC 6 cut(s) 71, 135, 192, 237, 378, 488
Bsp143I GATC 1 cut(s) 227
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 1 cut(s) 834
BspANI GGCC 6 cut(s) 125, 265, 272, 414, 794, 828
BspCNI CTCAG 4 cut(s) 84, 93, 178, 339
BspLI GGNNCC 1 cut(s) 132
BspMAI CTGCAG 3 cut(s) 514, 700, 718
BspMI ACCTGC 1 cut(s) 517
BspT104I TTCGAA 1 cut(s) 877
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 3 cut(s) 79, 283, 616
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 5 cut(s) 126, 314, 391, 392, 795
BssMI GATC 1 cut(s) 227
BssNAI GTATAC 1 cut(s) 899
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 1 cut(s) 314
Bst1107I GTATAC 1 cut(s) 899
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 3 cut(s) 127, 633, 796
Bst4CI ACNGT 4 cut(s) 490, 673, 808, 856
BstBAI YACGTR 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 877
BstC8I GCNNGC 5 cut(s) 192, 405, 529, 736, 987
BstDEI CTNAG 5 cut(s) 92, 101, 186, 326, 450
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 2 cut(s) 346, 744
BstHHI GCGC 2 cut(s) 533, 754
BstKTI GATC 1 cut(s) 230
BstMAI GTCTC 2 cut(s) 203, 845
BstMBI GATC 1 cut(s) 227
BstMWI GCNNNNNNNGC 4 cut(s) 241, 329, 604, 982
BstNI CCWGG 3 cut(s) 127, 633, 796
BstNSI RCATGY 4 cut(s) 407, 593, 695, 980
BstSCI CCNGG 5 cut(s) 125, 391, 392, 631, 794
BstSFI CTRYAG 4 cut(s) 510, 696, 714, 994
BstSLI GKGCMC 1 cut(s) 488
BstV1I GCAGC 6 cut(s) 322, 423, 433, 682, 710, 743
BstZ17I GTATAC 1 cut(s) 899
BsuRI GGCC 6 cut(s) 125, 265, 272, 414, 794, 828
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 2 cut(s) 346, 744
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 5 cut(s) 192, 405, 529, 736, 987
CfoI GCGC 2 cut(s) 533, 754
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 635
Cfr9I CCCGGG 1 cut(s) 392
CsiI ACCWGGT 1 cut(s) 631
Csp6I GTAC 1 cut(s) 256
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 5 cut(s) 92, 101, 186, 326, 450
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
EaeI YGGCCR 5 cut(s) 123, 263, 270, 412, 826
Ecl136II GAGCTC 2 cut(s) 190, 235
Eco130I CCWWGG 1 cut(s) 314
Eco24I GRGCYC 3 cut(s) 135, 192, 237
Eco32I GATATC 1 cut(s) 951
Eco47I GGWCC 1 cut(s) 635
Eco53kI GAGCTC 2 cut(s) 190, 235
Eco57I CTGAAG 2 cut(s) 443, 893
Eco72I CACGTG 1 cut(s) 261
Eco88I CYCGRG 3 cut(s) 134, 392, 771
EcoICRI GAGCTC 2 cut(s) 190, 235
EcoRI GAATTC 1 cut(s) 567
EcoRII CCWGG 3 cut(s) 125, 631, 794
EcoRV GATATC 1 cut(s) 951
EcoT14I CCWWGG 1 cut(s) 314
EcoT22I ATGCAT 1 cut(s) 405
EcoT38I GRGCYC 3 cut(s) 135, 192, 237
ErhI CCWWGG 1 cut(s) 314
Esp3I CGTCTC 2 cut(s) 203, 845
FalI AAGNNNNNCTT 2 cut(s) 871, 903
FaqI GGGAC 1 cut(s) 883
FauNDI CATATG 1 cut(s) 540
FblI GTMKAC 1 cut(s) 898
Fnu4HI GCNGC 8 cut(s) 11, 311, 437, 447, 696, 699, 732, 834
FokI GGATG 2 cut(s) 353, 731
FriOI GRGCYC 3 cut(s) 135, 192, 237
Fsp4HI GCNGC 8 cut(s) 11, 311, 437, 447, 696, 699, 732, 834
FspBI CTAG 4 cut(s) 62, 627, 788, 954
GlaI GCGC 2 cut(s) 532, 753
GluI GCNGC 8 cut(s) 11, 311, 437, 447, 696, 699, 732, 834
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 6 cut(s) 125, 265, 272, 414, 794, 828
HapII CCGG 3 cut(s) 86, 266, 393
HhaI GCGC 2 cut(s) 533, 754
Hin6I GCGC 2 cut(s) 531, 752
HinP1I GCGC 2 cut(s) 531, 752
HincII GTYRAC 1 cut(s) 98
HindII GTYRAC 1 cut(s) 98
HindIII AAGCTT 2 cut(s) 494, 880
HinfI GANTC 3 cut(s) 94, 103, 119
HpaII CCGG 3 cut(s) 86, 266, 393
Hpy166II GTNNAC 5 cut(s) 98, 258, 473, 689, 899
Hpy188I TCNGA 1 cut(s) 232
Hpy8I GTNNAC 5 cut(s) 98, 258, 473, 689, 899
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 917
HpyCH4III ACNGT 4 cut(s) 490, 673, 808, 856
HpyCH4IV ACGT 2 cut(s) 35, 260
HpyCH4V TGCA 9 cut(s) 310, 403, 512, 527, 607, 698, 716, 731, 989
HpyF10VI GCNNNNNNNGC 4 cut(s) 241, 329, 604, 982
HpyF3I CTNAG 5 cut(s) 92, 101, 186, 326, 450
HpySE526I ACGT 2 cut(s) 35, 260
HspAI GCGC 2 cut(s) 531, 752
Kzo9I GATC 1 cut(s) 227
LmnI GCTCC 7 cut(s) 88, 130, 232, 373, 443, 706, 759
Lsp1109I GCAGC 6 cut(s) 322, 423, 433, 682, 710, 743
LweI GCATC 2 cut(s) 331, 390
MabI ACCWGGT 1 cut(s) 631
MaeI CTAG 4 cut(s) 62, 627, 788, 954
MaeII ACGT 2 cut(s) 35, 260
MaeIII GTNAC 5 cut(s) 81, 427, 453, 667, 802
MalI GATC 1 cut(s) 229
MboI GATC 1 cut(s) 227
MboII GAAGA 1 cut(s) 409
MfeI CAATTG 1 cut(s) 618
MhlI GDGCHC 6 cut(s) 71, 135, 192, 237, 378, 488
MlsI TGGCCA 3 cut(s) 272, 414, 828
MluCI AATT 9 cut(s) 42, 218, 351, 359, 476, 567, 618, 723, 959
MluNI TGGCCA 3 cut(s) 272, 414, 828
MlyI GAGTC 3 cut(s) 103, 112, 113
MmeI TCCRAC 1 cut(s) 781
MnlI CCTC 9 cut(s) 159, 193, 196, 301, 508, 511, 544, 784, 795
Mox20I TGGCCA 3 cut(s) 272, 414, 828
Mph1103I ATGCAT 1 cut(s) 405
MscI TGGCCA 3 cut(s) 272, 414, 828
MseI TTAA 5 cut(s) 479, 545, 819, 864, 921
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 3 cut(s) 272, 414, 828
MspI CCGG 3 cut(s) 86, 266, 393
MspR9I CCNGG 5 cut(s) 127, 393, 394, 633, 796
MunI CAATTG 1 cut(s) 618
Mva1269I GAATGC 1 cut(s) 989
MvaI CCWGG 3 cut(s) 127, 633, 796
MwoI GCNNNNNNNGC 4 cut(s) 241, 329, 604, 982
NciI CCSGG 2 cut(s) 393, 394
NcoI CCATGG 1 cut(s) 314
NdeI CATATG 1 cut(s) 540
NdeII GATC 1 cut(s) 227
NlaIV GGNNCC 1 cut(s) 132
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 667
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 4 cut(s) 407, 593, 695, 980
NspV TTCGAA 1 cut(s) 877
PaeI GCATGC 1 cut(s) 407
PaeR7I CTCGAG 1 cut(s) 771
PciI ACATGT 1 cut(s) 589
PctI GAATGC 1 cut(s) 989
PflFI GACNNNGTC 1 cut(s) 633
PkrI GCNGC 8 cut(s) 12, 312, 438, 448, 697, 700, 733, 835
PleI GAGTC 3 cut(s) 102, 111, 113
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 3 cut(s) 102, 111, 113
Ppu21I YACGTR 1 cut(s) 261
PscI ACATGT 1 cut(s) 589
Psp124BI GAGCTC 2 cut(s) 192, 237
Psp6I CCWGG 3 cut(s) 125, 631, 794
PspCI CACGTG 1 cut(s) 261
PspGI CCWGG 3 cut(s) 125, 631, 794
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 635
PstI CTGCAG 3 cut(s) 514, 700, 718
PsyI GACNNNGTC 1 cut(s) 633
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 408
SacI GAGCTC 2 cut(s) 192, 237
SaqAI TTAA 5 cut(s) 479, 545, 819, 864, 921
SatI GCNGC 8 cut(s) 11, 311, 437, 447, 696, 699, 732, 834
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 1 cut(s) 635
SchI GAGTC 3 cut(s) 103, 112, 113
ScrFI CCNGG 5 cut(s) 127, 393, 394, 633, 796
SduI GDGCHC 6 cut(s) 71, 135, 192, 237, 378, 488
SexAI ACCWGGT 1 cut(s) 631
SfaNI GCATC 2 cut(s) 331, 390
SfcI CTRYAG 4 cut(s) 510, 696, 714, 994
Sfr274I CTCGAG 1 cut(s) 771
SfuI TTCGAA 1 cut(s) 877
SinI GGWCC 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 771
SmaI CCCGGG 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 464, 771
SmoI CTYRAG 2 cut(s) 464, 771
SphI GCATGC 1 cut(s) 407
Sse9I AATT 9 cut(s) 42, 218, 351, 359, 476, 567, 618, 723, 959
SsiI CCGC 1 cut(s) 834
SspMI CTAG 4 cut(s) 62, 627, 788, 954
SstI GAGCTC 2 cut(s) 192, 237
StyD4I CCNGG 5 cut(s) 125, 391, 392, 631, 794
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 4 cut(s) 490, 673, 808, 856
TaiI ACGT 2 cut(s) 38, 263
TaqI TCGA 5 cut(s) 32, 237, 772, 877, 891
TaqII GACCGA 1 cut(s) 730
TasI AATT 9 cut(s) 42, 218, 351, 359, 476, 567, 618, 723, 959
TatI WGTACW 1 cut(s) 255
TauI GCSGC 1 cut(s) 836
Tru1I TTAA 5 cut(s) 479, 545, 819, 864, 921
Tru9I TTAA 5 cut(s) 479, 545, 819, 864, 921
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 7 cut(s) 10, 310, 436, 446, 695, 698, 731
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 3 cut(s) 35, 211, 413
TspMI CCCGGG 1 cut(s) 392
Tth111I GACNNNGTC 1 cut(s) 633
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 1 cut(s) 567
XbaI TCTAGA 3 cut(s) 61, 626, 787
XceI RCATGY 4 cut(s) 407, 593, 695, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 771
XmaI CCCGGG 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 898
XspI CTAG 4 cut(s) 62, 627, 788, 954
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.