RLG00000023866

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015657
Physical Location & Seq
Forward (+)
29346962 .. 29349197
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023866

Sequence Viewer

Length: 1317 bp
ATGGAGGGGCTAAGTATCGTGTTTTTATTGCTACCGACGCTTGCAGTTCATGTATCATGGGGTCTAGAGAGCACTGCCGGTTCGCGACCGGAGCTGAGTCAATTGAGTCGGCAGTTGCTGGACTCGGCCAGGGAGCCCGAGTTCTTTGACTGGTTGACAAAAGTCAGGAGGAAAATCCACGAGAACCCTGAGCTCGCCTTTGAGGAGGAAGCGACCAGCCGATTCATACGATCGGAGCTCGACTCGCTTGGGATAGAGTACACGTGGCCGGTGGCCAAGACTGGGTTGGTGGCTTCCATCGGTTCTGGGTTTCAGCCATGGTTCGCTCTCCGGGCAGACATGGATGCTCTCCCAATTCAGTGGGAGCATAAGAGCAAGAACCCTGGAAAAATGCATGCATGTGGCCATGATGTTCATGTAACAATGATGCTTGGAGCTGCTAAGTTACTTCAGCTCAAGAGTAAACAATTAAAGGGCACAATTAAGCTTGTGTTTCAACCTGCAGAGGAGGGTCATGCAGGGGCATACCATATGTTAAAAGAGGGTGCTCTTGATGGAATTCATGGCATTTTTGGATTACATGTTTCGCCAGAAATGCCGACCGGTACTATTGGTTCTAGACCTGGTCCATTCCTAGCTGGTTCTGGTAGATTTTTAGTCACAGTTCAAGGAAAAGGTGGACATGCTGCAGCTCCCCATTTGACTGCCGACCCGATTCTTGCAGCCTGCTCGGTCATCCTTGCCCTCCAACAGCTTGTATCTCGAGAAACCGACCCTCTAGAGCCCAGGGTGGTAACTGTTGGGTTTGTTAAGGGTGGCCAAGCTGATAATGTAATCCCAGAATCGGTGTTACTTAAATTCACTTTTCGAAGCATGACTTTAGAAGGTCTACATTACCTTCAGCAAAGGATTAAAGAGGTCATAGAAATGCAAGCATCAGTGCACAAGTGTACCGCAACAACAGACTTCATGCTTGAGAAAATGAGGCCGTATCCACCTCTTGTAATTGATGAAGCACTGTATGAACATGGCAAGAGTGTAAGCGAAGCTTTGGTTGGGGAACCAAATGTGTATGTTCTTCCAATGTCTATGGGAGCAGAGGACTTCAGCTTCTATACACATCAGATGGCTGCCGCATTGTTCATGATTGGGACTAAAAACGATACTTTAGGGTCGAACACTACAGACTTACATTCACCTTTCTTGGTCATTGATGAGGAGGTACTTCCAATAGGAGCAGCTCTTCATGCTGCAGTTGCCATTTCATTCTTGGATAATACCACTGATGCAGCTGTCACTCAGTCGCTGACTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

439

Amino Acids

47.7

Weight (kDa)

5.69

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 109 - 420 9.1e-40 Peptidase family M20/M25/M40
M20_dimer PF07687 214 - 310 6.6e-14 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19710
malus_domestica MD12G1032400.v1.1
prunus_persica Prupe.7G099700_v2.0.a1 Prupe.7G099800_v2.0.a1
pyrus_communis pycom12g03000
rosa_chinensis RchiOBHm_Chr3g0475041
rosa_laevigata RLG00000023866 RLG00000023868
rosa_multiflora Rmu_sc0003093.1_g000013
rosa_rugosa Rorug03G0146400 Rorug03G0146400
rosa_samantha Rh3AG196100 Rh3BG226300 Rh3CG221300 Rh3DG221600
rosa_wichuraiana Rw3G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 889
AccII CGCG 1 cut(s) 85
AciI CCGC 2 cut(s) 954, 1134
AcoI YGGCCR 5 cut(s) 126, 266, 273, 403, 817
AcsI RAATTY 2 cut(s) 558, 857
AcuI CTGAAG 3 cut(s) 434, 884, 1090
AcvI CACGTG 1 cut(s) 264
AfaI GTAC 4 cut(s) 260, 607, 952, 1224
AfiI CCNNNNNNNGG 2 cut(s) 282, 844
AflIII ACRYGT 2 cut(s) 261, 580
AgeI ACCGGT 1 cut(s) 602
AgsI TTSAA 2 cut(s) 497, 668
AjnI CCWGG 4 cut(s) 128, 382, 622, 785
AjuI GAANNNNNNNTTGG 2 cut(s) 1038, 1070
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
Alw21I GWGCWC 5 cut(s) 74, 195, 240, 550, 945
Alw44I GTGCAC 1 cut(s) 941
AlwNI CAGNNNCTG 2 cut(s) 118, 1306
Ama87I CYCGRG 2 cut(s) 137, 762
AoxI GGCC 6 cut(s) 126, 266, 273, 403, 817, 986
ApaLI GTGCAC 1 cut(s) 941
ApeKI GCWGC 8 cut(s) 437, 686, 689, 722, 1130, 1238, 1250, 1289
ApoI RAATTY 2 cut(s) 558, 857
AsiGI ACCGGT 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 626
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 1188
AsuII TTCGAA 1 cut(s) 868
AvaI CYCGRG 2 cut(s) 137, 762
AvaII GGWCC 1 cut(s) 626
BaeGI GKGCMC 2 cut(s) 479, 945
BaeI ACNNNNGTAYC 2 cut(s) 934, 967
BalI TGGCCA 3 cut(s) 275, 405, 819
BanII GRGCYC 4 cut(s) 138, 195, 240, 786
BauI CACGAG 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 264
Bbv12I GWGCWC 5 cut(s) 74, 195, 240, 550, 945
BbvI GCAGC 8 cut(s) 424, 673, 701, 734, 1117, 1237, 1250, 1301
BccI CCATC 3 cut(s) 305, 548, 1120
BceAI ACGGC 1 cut(s) 973
BcgI CGANNNNNNTGC 2 cut(s) 711, 745
BciT130I CCWGG 4 cut(s) 130, 384, 624, 787
BciVI GTATCC 1 cut(s) 1002
BcnI CCSGG 1 cut(s) 332
BfaI CTAG 6 cut(s) 65, 618, 635, 779, 1311, 1315
BfmI CTRYAG 4 cut(s) 501, 687, 1182, 1251
BfuAI ACCTGC 1 cut(s) 508
BfuI GTATCC 1 cut(s) 1002
BisI GCNGC 9 cut(s) 438, 687, 690, 723, 1131, 1134, 1239, 1251, 1290
BlsI GCNGC 9 cut(s) 439, 688, 691, 724, 1132, 1135, 1240, 1252, 1291
Bme1390I CCNGG 5 cut(s) 130, 332, 384, 624, 787
Bme18I GGWCC 1 cut(s) 626
BmeT110I CYCGRG 2 cut(s) 137, 762
BmgT120I GGNCC 1 cut(s) 626
BmiI GGNNCC 2 cut(s) 135, 1062
BmrFI CCNGG 5 cut(s) 130, 332, 384, 624, 787
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 4 cut(s) 334, 417, 944, 1276
BmuI ACTGGG 1 cut(s) 291
BoxI GACNNNNGTC 1 cut(s) 161
BplI GAGNNNNNCTC 2 cut(s) 227, 259
Bpu10I CCTNAGC 1 cut(s) 189
Bpu14I TTCGAA 1 cut(s) 868
BpuEI CTTGAG 2 cut(s) 440, 995
BpuMI CCSGG 1 cut(s) 332
BsaAI YACGTR 1 cut(s) 264
BsaJI CCNNGG 5 cut(s) 129, 317, 382, 785, 786
BsaWI WCCGGW 2 cut(s) 88, 602
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 844
Bse118I RCCGGY 3 cut(s) 77, 268, 602
Bse1I ACTGG 2 cut(s) 155, 286
BseBI CCWGG 4 cut(s) 130, 384, 624, 787
BseDI CCNNGG 5 cut(s) 129, 317, 382, 785, 786
BseGI GGATG 2 cut(s) 349, 735
BseLI CCNNNNNNNGG 2 cut(s) 282, 844
BseMII CTCAG 3 cut(s) 86, 180, 1313
BseNI ACTGG 2 cut(s) 155, 286
BseRI GAGGAG 3 cut(s) 218, 521, 1232
BseSI GKGCMC 2 cut(s) 479, 945
BseXI GCAGC 8 cut(s) 424, 673, 701, 734, 1117, 1237, 1250, 1301
Bsh1236I CGCG 1 cut(s) 85
Bsh1285I CGRYCG 3 cut(s) 89, 233, 603
BshFI GGCC 6 cut(s) 128, 268, 275, 405, 819, 988
BshTI ACCGGT 1 cut(s) 602
BsiEI CGRYCG 3 cut(s) 89, 233, 603
BsiHKAI GWGCWC 5 cut(s) 74, 195, 240, 550, 945
BsiHKCI CYCGRG 2 cut(s) 137, 762
BsiSI CCGG 5 cut(s) 78, 89, 269, 331, 603
BslFI GGGAC 1 cut(s) 1165
BslI CCNNNNNNNGG 2 cut(s) 282, 844
BsmFI GGGAC 1 cut(s) 1165
BsnI GGCC 6 cut(s) 128, 268, 275, 405, 819, 988
BsoBI CYCGRG 2 cut(s) 137, 762
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 8 cut(s) 74, 138, 195, 240, 479, 550, 786, 945
Bsp143I GATC 1 cut(s) 230
Bsp19I CCATGG 1 cut(s) 317
Bsp68I TCGCGA 1 cut(s) 85
BspACI CCGC 2 cut(s) 954, 1134
BspANI GGCC 6 cut(s) 128, 268, 275, 405, 819, 988
BspCNI CTCAG 3 cut(s) 87, 181, 1312
BspFNI CGCG 1 cut(s) 85
BspHI TCATGA 1 cut(s) 1143
BspLI GGNNCC 2 cut(s) 135, 1062
BspMAI CTGCAG 3 cut(s) 505, 691, 1255
BspMI ACCTGC 1 cut(s) 508
BspQI GCTCTTC 1 cut(s) 1248
BspT104I TTCGAA 1 cut(s) 868
BsrFI RCCGGY 3 cut(s) 77, 268, 602
BsrI ACTGG 2 cut(s) 155, 286
BssAI RCCGGY 3 cut(s) 77, 268, 602
BssECI CCNNGG 5 cut(s) 129, 317, 382, 785, 786
BssMI GATC 1 cut(s) 230
BssSI CACGAG 1 cut(s) 179
BssT1I CCWWGG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 179
Bst2UI CCWGG 4 cut(s) 130, 384, 624, 787
Bst4CI ACNGT 3 cut(s) 664, 799, 1020
Bst6I CTCTTC 1 cut(s) 1248
BstBAI YACGTR 1 cut(s) 264
BstBI TTCGAA 1 cut(s) 868
BstC8I GCNNGC 5 cut(s) 42, 195, 396, 727, 933
BstDEI CTNAG 5 cut(s) 11, 95, 189, 441, 1299
BstDSI CCRYGG 1 cut(s) 317
BstF5I GGATG 2 cut(s) 349, 735
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 1 cut(s) 233
BstMBI GATC 1 cut(s) 230
BstMCI CGRYCG 3 cut(s) 89, 233, 603
BstMWI GCNNNNNNNGC 7 cut(s) 37, 91, 244, 332, 595, 1247, 1256
BstNI CCWGG 4 cut(s) 130, 384, 624, 787
BstNSI RCATGY 4 cut(s) 398, 402, 584, 686
BstPAI GACNNNNGTC 1 cut(s) 161
BstSCI CCNGG 5 cut(s) 128, 330, 382, 622, 785
BstSFI CTRYAG 4 cut(s) 501, 687, 1182, 1251
BstSLI GKGCMC 2 cut(s) 479, 945
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 8 cut(s) 424, 673, 701, 734, 1117, 1237, 1250, 1301
BstXI CCANNNNNNTGG 1 cut(s) 360
BsuI GTATCC 1 cut(s) 1002
BsuRI GGCC 6 cut(s) 128, 268, 275, 405, 819, 988
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 2 cut(s) 349, 735
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 5 cut(s) 72, 365, 945, 1016, 1281
BtuMI TCGCGA 1 cut(s) 85
BveI ACCTGC 1 cut(s) 508
Cac8I GCNNGC 5 cut(s) 42, 195, 396, 727, 933
CaiI CAGNNNCTG 2 cut(s) 118, 1306
CciI TCATGA 1 cut(s) 1143
Cfr10I RCCGGY 3 cut(s) 77, 268, 602
Cfr13I GGNCC 1 cut(s) 626
CseI GACGC 1 cut(s) 46
CsiI ACCWGGT 1 cut(s) 622
Csp6I GTAC 4 cut(s) 259, 606, 951, 1223
CspAI ACCGGT 1 cut(s) 602
CviQI GTAC 4 cut(s) 259, 606, 951, 1223
DdeI CTNAG 5 cut(s) 11, 95, 189, 441, 1299
DpnI GATC 1 cut(s) 232
DpnII GATC 1 cut(s) 230
EaeI YGGCCR 5 cut(s) 126, 266, 273, 403, 817
Eam1104I CTCTTC 1 cut(s) 1248
EarI CTCTTC 1 cut(s) 1248
Ecl136II GAGCTC 2 cut(s) 193, 238
Eco130I CCWWGG 1 cut(s) 317
Eco24I GRGCYC 4 cut(s) 138, 195, 240, 786
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 2 cut(s) 193, 238
Eco57I CTGAAG 3 cut(s) 434, 884, 1090
Eco72I CACGTG 1 cut(s) 264
Eco88I CYCGRG 2 cut(s) 137, 762
EcoICRI GAGCTC 2 cut(s) 193, 238
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 4 cut(s) 128, 382, 622, 785
EcoT14I CCWWGG 1 cut(s) 317
EcoT22I ATGCAT 2 cut(s) 396, 400
EcoT38I GRGCYC 4 cut(s) 138, 195, 240, 786
ErhI CCWWGG 1 cut(s) 317
FalI AAGNNNNNCTT 4 cut(s) 862, 894, 1033, 1065
FaqI GGGAC 1 cut(s) 1165
FauNDI CATATG 1 cut(s) 531
FblI GTMKAC 1 cut(s) 889
Fnu4HI GCNGC 9 cut(s) 438, 687, 690, 723, 1131, 1134, 1239, 1251, 1290
FokI GGATG 2 cut(s) 356, 722
FriOI GRGCYC 4 cut(s) 138, 195, 240, 786
Fsp4HI GCNGC 9 cut(s) 438, 687, 690, 723, 1131, 1134, 1239, 1251, 1290
FspBI CTAG 6 cut(s) 65, 618, 635, 779, 1311, 1315
GluI GCNGC 9 cut(s) 438, 687, 690, 723, 1131, 1134, 1239, 1251, 1290
HaeIII GGCC 6 cut(s) 128, 268, 275, 405, 819, 988
HapII CCGG 5 cut(s) 78, 89, 269, 331, 603
HgaI GACGC 1 cut(s) 46
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HindIII AAGCTT 2 cut(s) 485, 1047
HinfI GANTC 7 cut(s) 97, 106, 122, 222, 242, 715, 842
HpaII CCGG 5 cut(s) 78, 89, 269, 331, 603
HphI GGTGA 1 cut(s) 1188
Hpy166II GTNNAC 7 cut(s) 156, 261, 464, 680, 890, 943, 951
Hpy188I TCNGA 2 cut(s) 235, 1125
Hpy8I GTNNAC 7 cut(s) 156, 261, 464, 680, 890, 943, 951
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 2 cut(s) 878, 908
HpyCH4III ACNGT 3 cut(s) 664, 799, 1020
HpyCH4IV ACGT 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 7 cut(s) 37, 91, 244, 332, 595, 1247, 1256
HpyF3I CTNAG 5 cut(s) 11, 95, 189, 441, 1299
HpySE526I ACGT 1 cut(s) 263
Kzo9I GATC 1 cut(s) 230
LguI GCTCTTC 1 cut(s) 1248
LmnI GCTCC 8 cut(s) 91, 133, 235, 364, 434, 697, 1094, 1235
Lsp1109I GCAGC 8 cut(s) 424, 673, 701, 734, 1117, 1237, 1250, 1301
LweI GCATC 4 cut(s) 334, 417, 944, 1276
MabI ACCWGGT 1 cut(s) 622
MaeI CTAG 6 cut(s) 65, 618, 635, 779, 1311, 1315
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 6 cut(s) 418, 444, 658, 793, 849, 1294
MalI GATC 1 cut(s) 232
MboI GATC 1 cut(s) 230
MboII GAAGA 2 cut(s) 1070, 1235
MfeI CAATTG 1 cut(s) 101
MhlI GDGCHC 8 cut(s) 74, 138, 195, 240, 479, 550, 786, 945
MlsI TGGCCA 3 cut(s) 275, 405, 819
MluCI AATT 7 cut(s) 101, 354, 467, 480, 558, 857, 1005
MluNI TGGCCA 3 cut(s) 275, 405, 819
MlyI GAGTC 4 cut(s) 106, 115, 116, 236
MmeI TCCRAC 1 cut(s) 772
Mox20I TGGCCA 3 cut(s) 275, 405, 819
Mph1103I ATGCAT 2 cut(s) 396, 400
MscI TGGCCA 3 cut(s) 275, 405, 819
MseI TTAA 6 cut(s) 470, 483, 536, 810, 855, 912
MslI CAYNNNNRTG 2 cut(s) 399, 926
Msp20I TGGCCA 3 cut(s) 275, 405, 819
MspA1I CMGCKG 1 cut(s) 1292
MspI CCGG 5 cut(s) 78, 89, 269, 331, 603
MspR9I CCNGG 5 cut(s) 130, 332, 384, 624, 787
MunI CAATTG 1 cut(s) 101
MvaI CCWGG 4 cut(s) 130, 384, 624, 787
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 7 cut(s) 37, 91, 244, 332, 595, 1247, 1256
NciI CCSGG 1 cut(s) 332
NcoI CCATGG 1 cut(s) 317
NdeI CATATG 1 cut(s) 531
NdeII GATC 1 cut(s) 230
NlaIV GGNNCC 2 cut(s) 135, 1062
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 2 cut(s) 658, 1294
NruI TCGCGA 1 cut(s) 85
NsiI ATGCAT 2 cut(s) 396, 400
NspI RCATGY 4 cut(s) 398, 402, 584, 686
NspV TTCGAA 1 cut(s) 868
PaeI GCATGC 1 cut(s) 398
PaeR7I CTCGAG 1 cut(s) 762
PagI TCATGA 1 cut(s) 1143
PasI CCCWGGG 1 cut(s) 786
PciI ACATGT 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 1248
PfeI GAWTC 3 cut(s) 222, 715, 842
PflFI GACNNNGTC 1 cut(s) 624
PinAI ACCGGT 1 cut(s) 602
PkrI GCNGC 9 cut(s) 439, 688, 691, 724, 1132, 1135, 1240, 1252, 1291
Ple19I CGATCG 1 cut(s) 233
PleI GAGTC 4 cut(s) 105, 114, 116, 236
PmaCI CACGTG 1 cut(s) 264
PmlI CACGTG 1 cut(s) 264
PpsI GAGTC 4 cut(s) 105, 114, 116, 236
Ppu21I YACGTR 1 cut(s) 264
PscI ACATGT 1 cut(s) 580
PshAI GACNNNNGTC 1 cut(s) 161
Psp124BI GAGCTC 2 cut(s) 195, 240
Psp6I CCWGG 4 cut(s) 128, 382, 622, 785
PspCI CACGTG 1 cut(s) 264
PspGI CCWGG 4 cut(s) 128, 382, 622, 785
PspN4I GGNNCC 2 cut(s) 135, 1062
PspPI GGNCC 1 cut(s) 626
PsrI GAACNNNNNNTAC 2 cut(s) 598, 630
PstI CTGCAG 3 cut(s) 505, 691, 1255
PstNI CAGNNNCTG 2 cut(s) 118, 1306
PsyI GACNNNGTC 1 cut(s) 624
PvuI CGATCG 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 1292
RruI TCGCGA 1 cut(s) 85
RsaI GTAC 4 cut(s) 260, 607, 952, 1224
RsaNI GTAC 4 cut(s) 259, 606, 951, 1223
RseI CAYNNNNRTG 2 cut(s) 399, 926
SacI GAGCTC 2 cut(s) 195, 240
SapI GCTCTTC 1 cut(s) 1248
SaqAI TTAA 6 cut(s) 470, 483, 536, 810, 855, 912
SatI GCNGC 9 cut(s) 438, 687, 690, 723, 1131, 1134, 1239, 1251, 1290
Sau3AI GATC 1 cut(s) 230
Sau96I GGNCC 1 cut(s) 626
SchI GAGTC 4 cut(s) 106, 115, 116, 236
ScrFI CCNGG 5 cut(s) 130, 332, 384, 624, 787
SduI GDGCHC 8 cut(s) 74, 138, 195, 240, 479, 550, 786, 945
SexAI ACCWGGT 1 cut(s) 622
SfaNI GCATC 4 cut(s) 334, 417, 944, 1276
SfcI CTRYAG 4 cut(s) 501, 687, 1182, 1251
Sfr274I CTCGAG 1 cut(s) 762
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 762
SmiMI CAYNNNNRTG 2 cut(s) 399, 926
SmlI CTYRAG 3 cut(s) 455, 762, 974
SmoI CTYRAG 3 cut(s) 455, 762, 974
SphI GCATGC 1 cut(s) 398
Sse9I AATT 7 cut(s) 101, 354, 467, 480, 558, 857, 1005
SsiI CCGC 2 cut(s) 954, 1134
SspMI CTAG 6 cut(s) 65, 618, 635, 779, 1311, 1315
SstI GAGCTC 2 cut(s) 195, 240
StyD4I CCNGG 5 cut(s) 128, 330, 382, 622, 785
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 3 cut(s) 664, 799, 1020
TaiI ACGT 1 cut(s) 266
TaqI TCGA 4 cut(s) 240, 763, 868, 1175
TaqII GACCGA 1 cut(s) 721
TasI AATT 7 cut(s) 101, 354, 467, 480, 558, 857, 1005
TatI WGTACW 1 cut(s) 258
TauI GCSGC 1 cut(s) 1136
TfiI GAWTC 3 cut(s) 222, 715, 842
Tru1I TTAA 6 cut(s) 470, 483, 536, 810, 855, 912
Tru9I TTAA 6 cut(s) 470, 483, 536, 810, 855, 912
TscAI CASTG 5 cut(s) 79, 365, 945, 1023, 1288
TseFI GTSAC 2 cut(s) 658, 1294
TseI GCWGC 8 cut(s) 437, 686, 689, 722, 1130, 1238, 1250, 1289
Tsp45I GTSAC 2 cut(s) 658, 1294
TspRI CASTG 5 cut(s) 79, 365, 945, 1023, 1288
Tth111I GACNNNGTC 1 cut(s) 624
VneI GTGCAC 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 626
XapI RAATTY 2 cut(s) 558, 857
XbaI TCTAGA 3 cut(s) 64, 617, 778
XceI RCATGY 4 cut(s) 398, 402, 584, 686
XcmI CCANNNNNNNNNTGG 2 cut(s) 283, 1267
XhoI CTCGAG 1 cut(s) 762
XmiI GTMKAC 1 cut(s) 889
XspI CTAG 6 cut(s) 65, 618, 635, 779, 1311, 1315
Zsp2I ATGCAT 2 cut(s) 396, 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.