FvH4_6g20630

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014222
Physical Location & Seq
Forward (+)
14218163 .. 14219329
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20630.t1

Sequence Viewer

Length: 504 bp
ATGAACAACACAACTGACACAAGTGGTTTTCTCGGCTCTAACAACATTGGAGGATTCGGCTATGGCATTGGTGTATCAGTTGGCATCCTTCTTCTCATCACGACCATCACCCTTGCTTCCTATTTCTGCACCAGAACTCCTCCACCAGCGCACCCTGCAACGCGGGGGATCAACGCCCTCGACCCTCCAGACCTGCAAAGCTTTGTTGTAGATATAGGACTCGATGACAACACCATCAAGAGCTACCCGAAGATGCTATACTCCGAGGCCAAGCTACAGAAGACAGACTCCACTGCTTCCTGCTGCTCTATATGTCTAGCAGATTACAAGGCCACCGATACACTCCGGTTACTTCCAGATTGTGAACACCTCTTTCATCTCAAGTGCGTTGATCCATGGCTGCGGCTTCATCCTACATGTCCGGTTTGCAGAACATCTCCGCTTCCGACGCCTTTGTCGACTCCTCTAGCTGAGGTGGTGCCATTGGCAAGCAGAAGGGACTGA

Protein Analysis

168

Amino Acids

18.05

Weight (kDa)

6.03

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 98 - 144 8.4e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 101 - 144 1e-12 Ring finger domain
zf-RING_11 PF17123 102 - 130 8.2e-10 RING-like zinc finger
zf-C3HC4 PF00097 102 - 143 1.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 102 - 143 6.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35910
fragaria_vesca FvH4_6g20630
malus_domestica MD12G1042400.v1.1 MD14G1041600.v1.1
prunus_persica Prupe.7G092500_v2.0.a1
pyrus_communis pycom14g03530
rosa_chinensis RchiOBHm_Chr3g0476521
rosa_laevigata RLG00000023765
rosa_multiflora Rmu_sc0004556.1_g000005 Rmu_sc0015190.1_g000003
rosa_roxburghii Rroxscaffold_6G00405260
rosa_rugosa Rorug03G0154500
rosa_samantha Rh3AG205600 Rh3BG238000 Rh3CG231800 Rh3DG231700
rosa_wichuraiana Rw3G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 454
Acc36I ACCTGC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 478
AccI GTMKAC 1 cut(s) 458
AccII CGCG 1 cut(s) 163
AciI CCGC 3 cut(s) 163, 403, 440
AclWI GGATC 2 cut(s) 176, 386
AcyI GRCGYC 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 416
AluBI AGCT 4 cut(s) 201, 243, 274, 470
AluI AGCT 4 cut(s) 201, 243, 274, 470
AlwI GGATC 2 cut(s) 176, 386
AoxI GGCC 2 cut(s) 267, 330
ApeKI GCWGC 2 cut(s) 303, 400
AspLEI GCGC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 100
BanI GGYRCC 1 cut(s) 478
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BbsI GAAGAC 1 cut(s) 287
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 290, 387
BccI CCATC 2 cut(s) 113, 242
BfaI CTAG 2 cut(s) 317, 467
BfmI CTRYAG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 201
BisI GCNGC 3 cut(s) 304, 401, 404
BlsI GCNGC 3 cut(s) 305, 402, 405
BmiI GGNNCC 1 cut(s) 480
BmsI GCATC 2 cut(s) 93, 243
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 264, 395
BsaWI WCCGGW 2 cut(s) 345, 421
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
BseDI CCNNGG 2 cut(s) 264, 395
BseGI GGATG 2 cut(s) 84, 409
BseMII CTCAG 1 cut(s) 462
BseRI GAGGAG 2 cut(s) 129, 453
BseXI GCAGC 2 cut(s) 290, 387
BsgI GTGCAG 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 163
BshFI GGCC 2 cut(s) 269, 332
BshNI GGYRCC 1 cut(s) 478
BsiSI CCGG 2 cut(s) 346, 422
BsnI GGCC 2 cut(s) 269, 332
Bsp143I GATC 2 cut(s) 168, 391
Bsp19I CCATGG 1 cut(s) 395
BspACI CCGC 3 cut(s) 163, 403, 440
BspANI GGCC 2 cut(s) 269, 332
BspCNI CTCAG 1 cut(s) 463
BspFNI CGCG 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 480
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 2 cut(s) 176, 386
BspT107I GGYRCC 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 264, 395
BssMI GATC 2 cut(s) 168, 391
BssNI GRCGYC 1 cut(s) 449
BssT1I CCWWGG 1 cut(s) 395
BstACI GRCGYC 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 1 cut(s) 395
BstF5I GGATG 2 cut(s) 84, 409
BstFNI CGCG 1 cut(s) 163
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 2 cut(s) 171, 394
BstMBI GATC 2 cut(s) 168, 391
BstMWI GCNNNNNNNGC 2 cut(s) 155, 448
BstNSI RCATGY 1 cut(s) 420
BstSFI CTRYAG 1 cut(s) 275
BstUI CGCG 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 290, 387
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 269, 332
BtgI CCRYGG 1 cut(s) 395
BtsCI GGATG 2 cut(s) 84, 409
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 291
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 490
CfoI GCGC 1 cut(s) 151
CseI GACGC 1 cut(s) 457
CviAII CATG 2 cut(s) 396, 417
DdeI CTNAG 1 cut(s) 471
DpnI GATC 2 cut(s) 170, 393
DpnII GATC 2 cut(s) 168, 391
DrdI GACNNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 395
EcoT14I CCWWGG 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 2 cut(s) 399, 420
FaiI YATR 7 cut(s) 63, 215, 259, 311, 313, 397, 418
FatI CATG 2 cut(s) 395, 416
FauI CCCGC 1 cut(s) 156
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 3 cut(s) 304, 401, 404
FokI GGATG 2 cut(s) 71, 396
Fsp4HI GCNGC 3 cut(s) 304, 401, 404
FspBI CTAG 2 cut(s) 317, 467
GlaI GCGC 1 cut(s) 150
GluI GCNGC 3 cut(s) 304, 401, 404
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 269, 332
HapII CCGG 2 cut(s) 346, 422
HgaI GACGC 1 cut(s) 457
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 449
Hin1II CATG 2 cut(s) 399, 420
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 4 cut(s) 54, 219, 287, 460
HpaII CCGG 2 cut(s) 346, 422
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 365, 459
Hpy188I TCNGA 2 cut(s) 265, 447
Hpy188III TCNNGA 4 cut(s) 100, 188, 238, 356
Hpy8I GTNNAC 2 cut(s) 365, 459
Hpy99I CGWCG 1 cut(s) 451
HpyAV CCTTC 2 cut(s) 98, 489
HpyCH4V TGCA 4 cut(s) 129, 158, 196, 429
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 448
HpyF3I CTNAG 1 cut(s) 471
Hsp92I GRCGYC 1 cut(s) 449
Hsp92II CATG 2 cut(s) 399, 420
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 2 cut(s) 168, 391
LpnPI CCDG 9 cut(s) 145, 159, 168, 201, 206, 313, 359, 369, 435
Lsp1109I GCAGC 2 cut(s) 290, 387
LweI GCATC 2 cut(s) 93, 243
MaeI CTAG 2 cut(s) 317, 467
MaeIII GTNAC 1 cut(s) 348
MalI GATC 2 cut(s) 170, 393
MboI GATC 2 cut(s) 168, 391
MboII GAAGA 3 cut(s) 83, 262, 292
MlyI GAGTC 3 cut(s) 213, 281, 454
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 8 cut(s) 44, 150, 188, 195, 259, 380, 466, 474
MspI CCGG 2 cut(s) 346, 422
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 2 cut(s) 155, 448
NcoI CCATGG 1 cut(s) 395
NdeII GATC 2 cut(s) 168, 391
NlaIII CATG 2 cut(s) 399, 420
NlaIV GGNNCC 1 cut(s) 480
NmeAIII GCCGAG 1 cut(s) 12
NspI RCATGY 1 cut(s) 420
PciI ACATGT 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 455
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 3 cut(s) 305, 402, 405
PleI GAGTC 3 cut(s) 213, 281, 454
PpsI GAGTC 3 cut(s) 213, 281, 454
PscI ACATGT 1 cut(s) 416
PspN4I GGNNCC 1 cut(s) 480
SalI GTCGAC 1 cut(s) 457
SatI GCNGC 3 cut(s) 304, 401, 404
Sau3AI GATC 2 cut(s) 168, 391
SchI GAGTC 3 cut(s) 213, 281, 454
SetI ASST 7 cut(s) 195, 203, 245, 276, 372, 472, 477
SfaNI GCATC 2 cut(s) 93, 243
SfcI CTRYAG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SsiI CCGC 3 cut(s) 163, 403, 440
SspMI CTAG 2 cut(s) 317, 467
StyI CCWWGG 1 cut(s) 395
TaqI TCGA 3 cut(s) 180, 222, 458
TauI GCSGC 1 cut(s) 406
TfiI GAWTC 1 cut(s) 54
TscAI CASTG 1 cut(s) 298
TseI GCWGC 2 cut(s) 303, 400
TspDTI ATGAA 3 cut(s) 17, 365, 398
TspRI CASTG 1 cut(s) 298
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 2 cut(s) 317, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.