Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0014222

Physical Location & Seq

Genomic Coordinates Forward (+)

3947438 .. 3947947

Gene Structure 510 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1041600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 510 bp

ATGAACGCCACAAGCGACTCTGGCGGCTTCCTCGGTTCCAACAACATTGGTAGTTTCGGTTATGGCATTGGTGTATCAGTTGGCATCCTCCTCCTCATCACAACCATCACCCTTGCCTCCTACTTCTGCGCCAGAACTCAGCAGCCGCCTGCCCCGCCCACACGCAGGAACACTCGCGACGCCATGGATCCGCCCGGCTTGGAAAACTTTGTTGTGGACATAGGACTTGATGACAACACCATTCAGAGCTACCCGAAAATGCTCTACTCCGAGGCCAAGCTCCAAAAGAAAGACTCCACTGCCTCCTGCTGCTCCATATGTCTTGCGGACTACAAGAACAGCGACGTGCTGCGGCTACTGCCAGACTGTAGACACCTCTTCCACCTCAAGTGCATTGATCCATGGCTGCGGTTGAACCCTACGTGTCCGGTCTGCAGAACGTCTCCCATCCCAACGCCCTTGTCAACTCCTCTGGCCGAGGTGGTTCCATTGGCGAGCAGGCGTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

170

Amino Acids

18.42

Weight (kDa)

7.52

Isoelectric Point (pI)

47.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	99 - 146	1.4e-09	RING-H2 zinc finger domain
zf-RING_2	PF13639	103 - 146	3.4e-13	Ring finger domain
zf-RING_11	PF17123	104 - 132	1.2e-10	RING-like zinc finger
zf-C3HC4	PF00097	104 - 145	8.8e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2	PF13923	104 - 145	6e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35910
fragaria_vesca	FvH4_6g20630
malus_domestica	MD12G1042400.v1.1 MD14G1041600.v1.1
prunus_persica	Prupe.7G092500_v2.0.a1
pyrus_communis	pycom14g03530
rosa_chinensis	RchiOBHm_Chr3g0476521
rosa_laevigata	RLG00000023765
rosa_multiflora	Rmu_sc0004556.1_g000005 Rmu_sc0015190.1_g000003
rosa_roxburghii	Rroxscaffold_6G00405260
rosa_rugosa	Rorug03G0154500
rosa_samantha	Rh3AG205600 Rh3BG238000 Rh3CG231800 Rh3DG231700
rosa_wichuraiana	Rw3G018700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	370
AccII	CGCG	1 cut(s)	177
AciI	CCGC	7 cut(s)	24, 146, 155, 191, 326, 352, 409
AclWI	GGATC	3 cut(s)	182, 195, 392
AcoI	YGGCCR	1 cut(s)	474
AcyI	GRCGYC	1 cut(s)	180
AfiI	CCNNNNNNNGG	1 cut(s)	165
AflIII	ACRYGT	1 cut(s)	422
AgsI	TTSAA	1 cut(s)	415
AjiI	CACGTC	1 cut(s)	346
AluBI	AGCT	2 cut(s)	249, 280
AluI	AGCT	2 cut(s)	249, 280
Alw26I	GTCTC	1 cut(s)	447
AlwI	GGATC	3 cut(s)	182, 195, 392
AoxI	GGCC	2 cut(s)	273, 474
ApeKI	GCWGC	4 cut(s)	142, 309, 349, 406
AspLEI	GCGC	1 cut(s)	131
AsuC2I	CCSGG	1 cut(s)	195
AsuHPI	GGTGA	1 cut(s)	100
BamHI	GGATCC	1 cut(s)	187
BbvI	GCAGC	4 cut(s)	154, 296, 336, 393
BccI	CCATC	2 cut(s)	113, 455
BcnI	CCSGG	1 cut(s)	195
BcoDI	GTCTC	1 cut(s)	447
BfmI	CTRYAG	2 cut(s)	367, 433
BisI	GCNGC	7 cut(s)	25, 143, 146, 310, 350, 353, 407
BlsI	GCNGC	7 cut(s)	26, 144, 147, 311, 351, 354, 408
Bme1390I	CCNGG	1 cut(s)	195
BmgBI	CACGTC	1 cut(s)	346
BmiI	GGNNCC	3 cut(s)	37, 189, 486
BmrFI	CCNGG	1 cut(s)	195
BmsI	GCATC	1 cut(s)	93
BpuEI	CTTGAG	1 cut(s)	371
BpuMI	CCSGG	1 cut(s)	195
BsaAI	YACGTR	1 cut(s)	423
BsaHI	GRCGYC	1 cut(s)	180
BsaJI	CCNNGG	5 cut(s)	31, 183, 270, 401, 477
BsaWI	WCCGGW	1 cut(s)	427
Bsc4I	CCNNNNNNNGG	1 cut(s)	165
BseDI	CCNNGG	5 cut(s)	31, 183, 270, 401, 477
BseGI	GGATG	2 cut(s)	84, 447
BseLI	CCNNNNNNNGG	1 cut(s)	165
BseMII	CTCAG	1 cut(s)	152
BseRI	GAGGAG	3 cut(s)	80, 83, 459
BseXI	GCAGC	4 cut(s)	154, 296, 336, 393
Bsh1236I	CGCG	1 cut(s)	177
BshFI	GGCC	2 cut(s)	275, 476
BsiSI	CCGG	2 cut(s)	195, 428
BslI	CCNNNNNNNGG	1 cut(s)	165
BsmAI	GTCTC	1 cut(s)	447
BsmBI	CGTCTC	1 cut(s)	447
BsnI	GGCC	2 cut(s)	275, 476
Bsp143I	GATC	2 cut(s)	187, 397
Bsp19I	CCATGG	2 cut(s)	183, 401
Bsp68I	TCGCGA	1 cut(s)	177
BspACI	CCGC	7 cut(s)	24, 146, 155, 191, 326, 352, 409
BspANI	GGCC	2 cut(s)	275, 476
BspCNI	CTCAG	1 cut(s)	151
BspFNI	CGCG	1 cut(s)	177
BspLI	GGNNCC	3 cut(s)	37, 189, 486
BspMAI	CTGCAG	1 cut(s)	437
BspPI	GGATC	3 cut(s)	182, 195, 392
BssECI	CCNNGG	5 cut(s)	31, 183, 270, 401, 477
BssMI	GATC	2 cut(s)	187, 397
BssNI	GRCGYC	1 cut(s)	180
BssT1I	CCWWGG	2 cut(s)	183, 401
Bst4CI	ACNGT	1 cut(s)	368
Bst6I	CTCTTC	1 cut(s)	383
BstACI	GRCGYC	1 cut(s)	180
BstBAI	YACGTR	1 cut(s)	423
BstC8I	GCNNGC	3 cut(s)	150, 496, 500
BstDEI	CTNAG	1 cut(s)	138
BstDSI	CCRYGG	2 cut(s)	183, 401
BstF5I	GGATG	2 cut(s)	84, 447
BstFNI	CGCG	1 cut(s)	177
BstHHI	GCGC	1 cut(s)	131
BstKTI	GATC	2 cut(s)	190, 400
BstMAI	GTCTC	1 cut(s)	447
BstMBI	GATC	2 cut(s)	187, 397
BstMWI	GCNNNNNNNGC	3 cut(s)	21, 154, 358
BstSCI	CCNGG	1 cut(s)	193
BstSFI	CTRYAG	2 cut(s)	367, 433
BstUI	CGCG	1 cut(s)	177
BstV1I	GCAGC	4 cut(s)	154, 296, 336, 393
BstX2I	RGATCY	1 cut(s)	187
BstYI	RGATCY	1 cut(s)	187
BsuRI	GGCC	2 cut(s)	275, 476
BtgI	CCRYGG	2 cut(s)	183, 401
BtrI	CACGTC	1 cut(s)	346
BtsCI	GGATG	2 cut(s)	84, 447
BtsI	GCAGTG	1 cut(s)	297
BtsIMutI	CAGTG	1 cut(s)	297
BtuMI	TCGCGA	1 cut(s)	177
Cac8I	GCNNGC	3 cut(s)	150, 496, 500
CfoI	GCGC	1 cut(s)	131
CseI	GACGC	1 cut(s)	188
CviAII	CATG	2 cut(s)	184, 402
CviJI	RGCY	9 cut(s)	27, 145, 198, 249, 275, 280, 355, 406, 476
CviKI_1	RGCY	9 cut(s)	27, 145, 198, 249, 275, 280, 355, 406, 476
DdeI	CTNAG	1 cut(s)	138
DpnI	GATC	2 cut(s)	189, 399
DpnII	GATC	2 cut(s)	187, 397
EaeI	YGGCCR	1 cut(s)	474
Eam1104I	CTCTTC	1 cut(s)	383
EarI	CTCTTC	1 cut(s)	383
EciI	GGCGGA	1 cut(s)	180
Eco130I	CCWWGG	2 cut(s)	183, 401
EcoT14I	CCWWGG	2 cut(s)	183, 401
ErhI	CCWWGG	2 cut(s)	183, 401
Esp3I	CGTCTC	1 cut(s)	447
FaeI	CATG	2 cut(s)	187, 405
FaiI	YATR	6 cut(s)	63, 185, 221, 317, 319, 403
FatI	CATG	2 cut(s)	183, 401
FauI	CCCGC	1 cut(s)	162
FauNDI	CATATG	1 cut(s)	317
FblI	GTMKAC	1 cut(s)	370
Fnu4HI	GCNGC	7 cut(s)	25, 143, 146, 310, 350, 353, 407
FokI	GGATG	2 cut(s)	71, 434
Fsp4HI	GCNGC	7 cut(s)	25, 143, 146, 310, 350, 353, 407
GlaI	GCGC	1 cut(s)	130
GluI	GCNGC	7 cut(s)	25, 143, 146, 310, 350, 353, 407
HaeIII	GGCC	2 cut(s)	275, 476
HapII	CCGG	2 cut(s)	195, 428
HgaI	GACGC	1 cut(s)	188
HhaI	GCGC	1 cut(s)	131
Hin1I	GRCGYC	1 cut(s)	180
Hin1II	CATG	2 cut(s)	187, 405
Hin6I	GCGC	1 cut(s)	129
HinP1I	GCGC	1 cut(s)	129
HincII	GTYRAC	1 cut(s)	465
HindII	GTYRAC	1 cut(s)	465
HinfI	GANTC	2 cut(s)	17, 293
HpaII	CCGG	2 cut(s)	195, 428
HphI	GGTGA	1 cut(s)	100
Hpy166II	GTNNAC	3 cut(s)	217, 371, 465
Hpy188I	TCNGA	2 cut(s)	246, 271
Hpy188III	TCNNGA	1 cut(s)	176
Hpy8I	GTNNAC	3 cut(s)	217, 371, 465
Hpy99I	CGWCG	2 cut(s)	182, 347
HpyCH4III	ACNGT	1 cut(s)	368
HpyCH4IV	ACGT	3 cut(s)	345, 422, 440
HpyCH4V	TGCA	2 cut(s)	393, 435
HpyF10VI	GCNNNNNNNGC	3 cut(s)	21, 154, 358
HpyF3I	CTNAG	1 cut(s)	138
HpySE526I	ACGT	3 cut(s)	345, 422, 440
Hsp92I	GRCGYC	1 cut(s)	180
Hsp92II	CATG	2 cut(s)	187, 405
HspAI	GCGC	1 cut(s)	129
Kzo9I	GATC	2 cut(s)	187, 397
LmnI	GCTCC	2 cut(s)	285, 317
Lsp1109I	GCAGC	4 cut(s)	154, 296, 336, 393
LweI	GCATC	1 cut(s)	93
MaeII	ACGT	3 cut(s)	345, 422, 440
MaeIII	GTNAC	1 cut(s)	503
MalI	GATC	2 cut(s)	189, 399
MboI	GATC	2 cut(s)	187, 397
MboII	GAAGA	1 cut(s)	370
MflI	RGATCY	1 cut(s)	187
MlyI	GAGTC	2 cut(s)	11, 287
MmeI	TCCRAC	1 cut(s)	63
MspI	CCGG	2 cut(s)	195, 428
MspR9I	CCNGG	1 cut(s)	195
MvnI	CGCG	1 cut(s)	177
MwoI	GCNNNNNNNGC	3 cut(s)	21, 154, 358
NciI	CCSGG	1 cut(s)	195
NcoI	CCATGG	2 cut(s)	183, 401
NdeI	CATATG	1 cut(s)	317
NdeII	GATC	2 cut(s)	187, 397
NlaIII	CATG	2 cut(s)	187, 405
NlaIV	GGNNCC	3 cut(s)	37, 189, 486
NmeAIII	GCCGAG	1 cut(s)	502
NmuCI	GTSAC	1 cut(s)	503
NruI	TCGCGA	1 cut(s)	177
PcsI	WCGNNNNNNNCGW	1 cut(s)	12
PkrI	GCNGC	7 cut(s)	26, 144, 147, 311, 351, 354, 408
PleI	GAGTC	2 cut(s)	11, 287
PpsI	GAGTC	2 cut(s)	11, 287
Ppu21I	YACGTR	1 cut(s)	423
PspN4I	GGNNCC	3 cut(s)	37, 189, 486
PstI	CTGCAG	1 cut(s)	437
PsuI	RGATCY	1 cut(s)	187
RruI	TCGCGA	1 cut(s)	177
SatI	GCNGC	7 cut(s)	25, 143, 146, 310, 350, 353, 407
Sau3AI	GATC	2 cut(s)	187, 397
SchI	GAGTC	2 cut(s)	11, 287
ScrFI	CCNGG	1 cut(s)	195
SetI	ASST	8 cut(s)	251, 282, 348, 378, 387, 425, 443, 483
SfaNI	GCATC	1 cut(s)	93
SfcI	CTRYAG	2 cut(s)	367, 433
SmlI	CTYRAG	1 cut(s)	386
SmoI	CTYRAG	1 cut(s)	386
SsiI	CCGC	7 cut(s)	24, 146, 155, 191, 326, 352, 409
StyD4I	CCNGG	1 cut(s)	193
StyI	CCWWGG	2 cut(s)	183, 401
TaaI	ACNGT	1 cut(s)	368
TaiI	ACGT	3 cut(s)	348, 425, 443
TauI	GCSGC	3 cut(s)	27, 148, 355
TscAI	CASTG	1 cut(s)	304
TseFI	GTSAC	1 cut(s)	503
TseI	GCWGC	4 cut(s)	142, 309, 349, 406
Tsp45I	GTSAC	1 cut(s)	503
TspDTI	ATGAA	1 cut(s)	17
TspRI	CASTG	1 cut(s)	304
XmiI	GTMKAC	1 cut(s)	370

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.