RLG00000023765

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014222
Physical Location & Seq
Reverse (-)
28001071 .. 28001583
513 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023765

Sequence Viewer

Length: 513 bp
ATGAACAGCACGAGTGACATGAACAGCACGAGTGGTTTTCTCGGCTCTAACAACATCGGCGGATTTGGCTATGGCATTGGTGTATCAGTTGGCATCCTTCTTCTCATCACAACCATCACCCTTGCTTCCTACTTCTGCACCAGAACTCCGCCACCAACGCACCCTGCAACGCCGAGGATTAACGCCCACGACCCTCCTGACCTGCAAAGCTTTGTTGTAGATATAGGACTCGATGACAACACTATCAAGAGCTACCCGAAGATGCTCTACTCCGAGGCCAAGCTGCAGAAGACAGGCTCCACTGCTTCCTGCTGCTCTATATGTCTAGCAGATTACAAGGCCACTGATACACTCCGGTTACTTCCAGACTGTGAACACCTCTTTCATCTCAAGTGTGTTGATCCATGGTTGCGGCTTCATCCTACCTGTCCGGTCTGCAGAACATCTCCACTTCCAACGCCTCTGTCGACTCCTCTGGCTGAGGTGGTGCCATTGGCAAGCAGAAGAGACTGA

Protein Analysis

171

Amino Acids

18.38

Weight (kDa)

6.49

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 102 - 147 8.4e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 104 - 147 1e-12 Ring finger domain
zf-RING_11 PF17123 105 - 133 8.4e-10 RING-like zinc finger
zf-C3HC4 PF00097 105 - 146 2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 105 - 146 6.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35910
fragaria_vesca FvH4_6g20630
malus_domestica MD12G1042400.v1.1 MD14G1041600.v1.1
prunus_persica Prupe.7G092500_v2.0.a1
pyrus_communis pycom14g03530
rosa_chinensis RchiOBHm_Chr3g0476521
rosa_laevigata RLG00000023765
rosa_multiflora Rmu_sc0004556.1_g000005 Rmu_sc0015190.1_g000003
rosa_roxburghii Rroxscaffold_6G00405260
rosa_rugosa Rorug03G0154500
rosa_samantha Rh3AG205600 Rh3BG238000 Rh3CG231800 Rh3DG231700
rosa_wichuraiana Rw3G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 487
AccI GTMKAC 1 cut(s) 467
AciI CCGC 3 cut(s) 60, 149, 412
AclWI GGATC 1 cut(s) 395
AluBI AGCT 3 cut(s) 210, 252, 283
AluI AGCT 3 cut(s) 210, 252, 283
Alw26I GTCTC 1 cut(s) 501
AlwI GGATC 1 cut(s) 395
AoxI GGCC 2 cut(s) 276, 339
ApeKI GCWGC 2 cut(s) 283, 312
AsuHPI GGTGA 1 cut(s) 109
BanI GGYRCC 1 cut(s) 487
BarI GAAGNNNNNNTAC 2 cut(s) 251, 283
BauI CACGAG 2 cut(s) 10, 28
BbsI GAAGAC 1 cut(s) 296
BbvCI CCTCAGC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 270, 299
BccI CCATC 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 501
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 2 cut(s) 284, 436
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 3 cut(s) 284, 313, 413
BlsI GCNGC 3 cut(s) 285, 314, 414
BmiI GGNNCC 2 cut(s) 298, 489
BmsI GCATC 2 cut(s) 102, 252
BpiI GAAGAC 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 173, 273, 404
BsaWI WCCGGW 2 cut(s) 354, 430
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
BseDI CCNNGG 3 cut(s) 173, 273, 404
BseGI GGATG 2 cut(s) 93, 418
BseMII CTCAG 1 cut(s) 471
BseRI GAGGAG 1 cut(s) 462
BseXI GCAGC 2 cut(s) 270, 299
BsgI GTGCAG 1 cut(s) 121
BshFI GGCC 2 cut(s) 278, 341
BshNI GGYRCC 1 cut(s) 487
BsiSI CCGG 2 cut(s) 355, 431
BsmAI GTCTC 1 cut(s) 501
BsnI GGCC 2 cut(s) 278, 341
Bsp143I GATC 1 cut(s) 400
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 3 cut(s) 60, 149, 412
BspANI GGCC 2 cut(s) 278, 341
BspCNI CTCAG 1 cut(s) 472
BspLI GGNNCC 2 cut(s) 298, 489
BspMAI CTGCAG 2 cut(s) 288, 440
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 487
BssECI CCNNGG 3 cut(s) 173, 273, 404
BssMI GATC 1 cut(s) 400
BssSI CACGAG 2 cut(s) 10, 28
BssT1I CCWWGG 1 cut(s) 404
Bst2BI CACGAG 2 cut(s) 10, 28
Bst4CI ACNGT 1 cut(s) 371
Bst6I CTCTTC 1 cut(s) 499
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 1 cut(s) 480
BstDSI CCRYGG 1 cut(s) 404
BstF5I GGATG 2 cut(s) 93, 418
BstKTI GATC 1 cut(s) 403
BstMAI GTCTC 1 cut(s) 501
BstMBI GATC 1 cut(s) 400
BstMWI GCNNNNNNNGC 2 cut(s) 66, 157
BstSFI CTRYAG 2 cut(s) 284, 436
BstV1I GCAGC 2 cut(s) 270, 299
BstV2I GAAGAC 1 cut(s) 296
BsuRI GGCC 2 cut(s) 278, 341
BtgI CCRYGG 1 cut(s) 404
BtsCI GGATG 2 cut(s) 93, 418
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 2 cut(s) 300, 342
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 499
CviAII CATG 2 cut(s) 19, 405
DdeI CTNAG 1 cut(s) 480
DpnI GATC 1 cut(s) 402
DpnII GATC 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 499
EarI CTCTTC 1 cut(s) 499
EciI GGCGGA 2 cut(s) 75, 138
Eco130I CCWWGG 1 cut(s) 404
EcoT14I CCWWGG 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 2 cut(s) 22, 408
FaiI YATR 6 cut(s) 20, 72, 224, 320, 322, 406
FatI CATG 2 cut(s) 18, 404
FblI GTMKAC 1 cut(s) 467
Fnu4HI GCNGC 3 cut(s) 284, 313, 413
FokI GGATG 2 cut(s) 80, 405
Fsp4HI GCNGC 3 cut(s) 284, 313, 413
FspBI CTAG 1 cut(s) 326
GluI GCNGC 3 cut(s) 284, 313, 413
HaeIII GGCC 2 cut(s) 278, 341
HapII CCGG 2 cut(s) 355, 431
Hin1II CATG 2 cut(s) 22, 408
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 2 cut(s) 228, 469
HpaII CCGG 2 cut(s) 355, 431
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 374, 468
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 3 cut(s) 197, 247, 365
Hpy8I GTNNAC 2 cut(s) 374, 468
HpyAV CCTTC 1 cut(s) 107
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 5 cut(s) 138, 167, 205, 286, 438
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 157
HpyF3I CTNAG 1 cut(s) 480
Hsp92II CATG 2 cut(s) 22, 408
Kzo9I GATC 1 cut(s) 400
LmnI GCTCC 1 cut(s) 302
Lsp1109I GCAGC 2 cut(s) 270, 299
LweI GCATC 2 cut(s) 102, 252
MaeI CTAG 1 cut(s) 326
MaeIII GTNAC 2 cut(s) 14, 357
MalI GATC 1 cut(s) 402
MboI GATC 1 cut(s) 400
MboII GAAGA 3 cut(s) 92, 271, 301
MlyI GAGTC 2 cut(s) 222, 463
MmeI TCCRAC 1 cut(s) 479
MnlI CCTC 7 cut(s) 168, 204, 268, 389, 471, 475, 483
MseI TTAA 1 cut(s) 180
MspI CCGG 2 cut(s) 355, 431
MwoI GCNNNNNNNGC 2 cut(s) 66, 157
NcoI CCATGG 1 cut(s) 404
NdeII GATC 1 cut(s) 400
NlaIII CATG 2 cut(s) 22, 408
NlaIV GGNNCC 2 cut(s) 298, 489
NmeAIII GCCGAG 2 cut(s) 21, 198
NmuCI GTSAC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 464
PkrI GCNGC 3 cut(s) 285, 314, 414
PleI GAGTC 2 cut(s) 222, 463
PpsI GAGTC 2 cut(s) 222, 463
PspN4I GGNNCC 2 cut(s) 298, 489
PstI CTGCAG 2 cut(s) 288, 440
SalI GTCGAC 1 cut(s) 466
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 3 cut(s) 284, 313, 413
Sau3AI GATC 1 cut(s) 400
SchI GAGTC 2 cut(s) 222, 463
SetI ASST 7 cut(s) 204, 212, 254, 285, 381, 428, 486
SfaNI GCATC 2 cut(s) 102, 252
SfcI CTRYAG 2 cut(s) 284, 436
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SsiI CCGC 3 cut(s) 60, 149, 412
SspMI CTAG 1 cut(s) 326
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 371
TaqI TCGA 2 cut(s) 231, 467
TauI GCSGC 1 cut(s) 415
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 2 cut(s) 307, 349
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 2 cut(s) 283, 312
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 4 cut(s) 17, 35, 374, 407
TspRI CASTG 2 cut(s) 307, 349
XmiI GTMKAC 1 cut(s) 467
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.