Rroxscaffold_6G00405260

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014222
Physical Location & Seq
Reverse (-)
27662882 .. 27663837
956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00405260.1

Sequence Viewer

Length: 504 bp
ATGAACAGCACGAGTGACACGAGTGGTTTTCTCGGCTCTACCAACATCGGCGGATTTGGCTATGGCATTGGTGTATCAGTTGGCATCCTTCTTCTCATCACAACCATCACCCTTGCTTCCTACTTCTGCACCAGAACTCCGCCACCAACGCACCCTGCAACGCCGAGGATTAACGCCCACGACCCTCCTGACCTGCAAAGCTTTGTTGTAGATATAGGACTCGATGACAACACTATCAAGAGCTACCCGAAGATGCTCTACTCCGAGGCCAAGCTGCAGAAGACAGGCTCCACTGCTTCCTGCTGCTCTATATGTCTAGCAGATTACAAGGCCACTGATACACTCCGGTTACTTCCAGACTGTGAACACCTCTTTCATCTCAAGTGTGTTGATCCATGGTTGCGGCTTCATCCTACCTGTCCGGTCTGCAGAACATCTCCGCTTCCAACGCCTCTGTCGACTCCTCTGGCTGAGGTGGTGCCATTGGCAAGCAGAAGAGACTGA

Protein Analysis

167

Amino Acids

18.03

Weight (kDa)

6.49

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 99 - 144 8.2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 101 - 144 1e-12 Ring finger domain
zf-RING_11 PF17123 102 - 130 8.2e-10 RING-like zinc finger
zf-C3HC4 PF00097 102 - 143 1.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 102 - 143 6.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35910
fragaria_vesca FvH4_6g20630
malus_domestica MD12G1042400.v1.1 MD14G1041600.v1.1
prunus_persica Prupe.7G092500_v2.0.a1
pyrus_communis pycom14g03530
rosa_chinensis RchiOBHm_Chr3g0476521
rosa_laevigata RLG00000023765
rosa_multiflora Rmu_sc0004556.1_g000005 Rmu_sc0015190.1_g000003
rosa_roxburghii Rroxscaffold_6G00405260
rosa_rugosa Rorug03G0154500
rosa_samantha Rh3AG205600 Rh3BG238000 Rh3CG231800 Rh3DG231700
rosa_wichuraiana Rw3G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 201
AccB1I GGYRCC 1 cut(s) 478
AccI GTMKAC 1 cut(s) 458
AciI CCGC 4 cut(s) 51, 140, 403, 440
AclWI GGATC 1 cut(s) 386
AluBI AGCT 3 cut(s) 201, 243, 274
AluI AGCT 3 cut(s) 201, 243, 274
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 1 cut(s) 386
AoxI GGCC 2 cut(s) 267, 330
ApeKI GCWGC 2 cut(s) 274, 303
AsuHPI GGTGA 1 cut(s) 100
BanI GGYRCC 1 cut(s) 478
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BauI CACGAG 2 cut(s) 10, 19
BbsI GAAGAC 1 cut(s) 287
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 261, 290
BccI CCATC 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 2 cut(s) 275, 427
BfuAI ACCTGC 1 cut(s) 201
BisI GCNGC 3 cut(s) 275, 304, 404
BlsI GCNGC 3 cut(s) 276, 305, 405
BmiI GGNNCC 2 cut(s) 289, 480
BmsI GCATC 2 cut(s) 93, 243
BpiI GAAGAC 1 cut(s) 287
Bpu10I CCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 365
BsaJI CCNNGG 3 cut(s) 164, 264, 395
BsaWI WCCGGW 2 cut(s) 345, 421
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
BseDI CCNNGG 3 cut(s) 164, 264, 395
BseGI GGATG 2 cut(s) 84, 409
BseMII CTCAG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 453
BseXI GCAGC 2 cut(s) 261, 290
BsgI GTGCAG 1 cut(s) 112
BshFI GGCC 2 cut(s) 269, 332
BshNI GGYRCC 1 cut(s) 478
BsiSI CCGG 2 cut(s) 346, 422
BsmAI GTCTC 1 cut(s) 492
BsnI GGCC 2 cut(s) 269, 332
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 395
BspACI CCGC 4 cut(s) 51, 140, 403, 440
BspANI GGCC 2 cut(s) 269, 332
BspCNI CTCAG 1 cut(s) 463
BspLI GGNNCC 2 cut(s) 289, 480
BspMAI CTGCAG 2 cut(s) 279, 431
BspMI ACCTGC 1 cut(s) 201
BspPI GGATC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 478
BssECI CCNNGG 3 cut(s) 164, 264, 395
BssMI GATC 1 cut(s) 391
BssSI CACGAG 2 cut(s) 10, 19
BssT1I CCWWGG 1 cut(s) 395
Bst2BI CACGAG 2 cut(s) 10, 19
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 1 cut(s) 490
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 1 cut(s) 395
BstF5I GGATG 2 cut(s) 84, 409
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 3 cut(s) 57, 148, 448
BstSFI CTRYAG 2 cut(s) 275, 427
BstV1I GCAGC 2 cut(s) 261, 290
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 2 cut(s) 269, 332
BtgI CCRYGG 1 cut(s) 395
BtsCI GGATG 2 cut(s) 84, 409
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 2 cut(s) 291, 333
BveI ACCTGC 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 490
CviAII CATG 1 cut(s) 396
DdeI CTNAG 1 cut(s) 471
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 490
EciI GGCGGA 2 cut(s) 66, 129
Eco130I CCWWGG 1 cut(s) 395
EcoT14I CCWWGG 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 395
FaeI CATG 1 cut(s) 399
FaiI YATR 5 cut(s) 63, 215, 311, 313, 397
FatI CATG 1 cut(s) 395
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 3 cut(s) 275, 304, 404
FokI GGATG 2 cut(s) 71, 396
Fsp4HI GCNGC 3 cut(s) 275, 304, 404
FspBI CTAG 1 cut(s) 317
GluI GCNGC 3 cut(s) 275, 304, 404
HaeIII GGCC 2 cut(s) 269, 332
HapII CCGG 2 cut(s) 346, 422
Hin1II CATG 1 cut(s) 399
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 2 cut(s) 219, 460
HpaII CCGG 2 cut(s) 346, 422
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 2 cut(s) 365, 459
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 3 cut(s) 188, 238, 356
Hpy8I GTNNAC 2 cut(s) 365, 459
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4V TGCA 5 cut(s) 129, 158, 196, 277, 429
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 148, 448
HpyF3I CTNAG 1 cut(s) 471
Hsp92II CATG 1 cut(s) 399
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 2 cut(s) 261, 290
LweI GCATC 2 cut(s) 93, 243
MaeI CTAG 1 cut(s) 317
MaeIII GTNAC 2 cut(s) 14, 348
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 3 cut(s) 83, 262, 292
MlyI GAGTC 2 cut(s) 213, 454
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 7 cut(s) 159, 195, 259, 380, 462, 466, 474
MseI TTAA 1 cut(s) 171
MspI CCGG 2 cut(s) 346, 422
MwoI GCNNNNNNNGC 3 cut(s) 57, 148, 448
NcoI CCATGG 1 cut(s) 395
NdeII GATC 1 cut(s) 391
NlaIII CATG 1 cut(s) 399
NlaIV GGNNCC 2 cut(s) 289, 480
NmeAIII GCCGAG 2 cut(s) 12, 189
NmuCI GTSAC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 455
PkrI GCNGC 3 cut(s) 276, 305, 405
PleI GAGTC 2 cut(s) 213, 454
PpsI GAGTC 2 cut(s) 213, 454
PspN4I GGNNCC 2 cut(s) 289, 480
PstI CTGCAG 2 cut(s) 279, 431
SalI GTCGAC 1 cut(s) 457
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 3 cut(s) 275, 304, 404
Sau3AI GATC 1 cut(s) 391
SchI GAGTC 2 cut(s) 213, 454
SetI ASST 7 cut(s) 195, 203, 245, 276, 372, 419, 477
SfaNI GCATC 2 cut(s) 93, 243
SfcI CTRYAG 2 cut(s) 275, 427
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SsiI CCGC 4 cut(s) 51, 140, 403, 440
SspMI CTAG 1 cut(s) 317
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 1 cut(s) 362
TaqI TCGA 2 cut(s) 222, 458
TauI GCSGC 1 cut(s) 406
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 2 cut(s) 298, 340
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 2 cut(s) 274, 303
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 3 cut(s) 17, 365, 398
TspRI CASTG 2 cut(s) 298, 340
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.