Rorug03G0154500

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014222
Physical Location & Seq
Reverse (-)
12699821 .. 12706669
6849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0154500.1

Sequence Viewer

Length: 3804 bp
ATGGCAGAACTTCTGCTCACTTTTGCTGCCGAGGGAATACTGACCAAGGTGACTTCGCTTGCTGCTCAAGAATTCACTCTCGTATTGGGTTTCAAAGGAGATCTAGCACAACTGCGTGACTCGTTCCTCAAGATTCAAGCTATGCTAAGAGATGTCGAGCATTCACAAGTTCGCGGGGAGACTGTGGAATTGTGGGTGAAAGATCTTGAAGACATAGCTCAAGAAGCTGATGATGTCTTGGATGAAATTGGGTATGAAGTTCTCCGACGCAAAGTAGAACTCCGTAACGAGATGAAGAAAAAGGTCAAAAATTTCTTTTCACACAACAATCCCATTGCATTTCGCTTTAAAATGGCACATAAAATTAAAAAGATCAACAAATCTTTGGTGAATTTGAACAATGAGGCAGCTGGCCGTATTGGGTTGGTTGCTAGGACATTAGCTAACGCAACCTCTCAAGATGTTGCAGTGCTTGACAGGGAAACCGTCTCCGACTTTATTGATATCGATGAGAAGTTCATCATTGGAAGGGAGGAGGTCGTGTCAGATGTAGTCAAAACATTGATCAAGTCAAACTATAATGAAGAAAATTATCTTCCGGTTCTGGCTATTGTGGGAATGCCAGGTCTAGGTAAGACAACTTTGGCTAAATCAATCTATCATGAAGCTGAGATTGATAGTCACTTCCATAAAAAAATATGGGTGTGTGTATCCACCCCGTTTGAAGTCAAGAAGATCTTAAGTGGAATTTTGGAATATTTTGAACCAGAGAAAGCCACAATACAAAGCAAGGATGCGATTTGTAAATTCATTCGAGAAAAGCTGGAAAATAAAAGATACCTTCTAGTGCTTGATGATGTTTGGAGCGAAGATCCAGAGAAATGGAAAGAGTTGAGGAGTTGTTTGCTAACAATTAAAGACACTCGAGGAAGCAGCATCATTGTCACTACCCGTAGTGACAAAGTTGCAAAAGTCATGGAGACCCTTCCTAGGCCTGATTTGAGAAGATTATCAGATGACGAATGTTGGCTCATAATCAAGGATAAAGCAGTTTCAGTTGGGAGTGCTCCTATGTCCAAAGAGCAGGAGACAACGGGTAAAGAGATTGCTAAGAAGTGCGGAGGTGTACCATTAATGGCAAAGGTTTTGGGAGATACGCTGCGCTCTAAAACAAGAGATGAATGGGGATCAATTGTAAACAGTAAAGTATGGGATTCACCAGTAGGAGAAAAGAGAATTCTTTCAATTTTGAAGTTAAGTTTTGATGAATTGAAACCTCCATCCTTAAAACAATGTTTTGCATATTGCTCGATGCTCTTCAAGGATTTTGATATTGAAAAGGATGACTTGATCCAACTTTGGATGGCTCAAGGGTTGCTTCACCCCTGTTCTGACAAAAACCTAGAGATGGAGGAGAGAGGTAATGAATATTTTAAAATCCTATTGGCGAAGTCTTTTTTCCAAGACGTTACAAAGGATTATGGGAGTAATGTTACCAAATGTAAGATGCACGATCTTGTGCATGATTTTGCAGAACGTGTATCAAAAGCGGGGAACTTACGCTCACTTTTTTCAAATGGAGAAGTCCTTGAGAGCAACTTACTTAACTTGAAATATTTACGTGTCTTAAATTTATACAAAGCCGACATTATGGAGTTGCCAATTTCAATTGGGAAGTTGAAACATTTGAGGTATTTGAATGTTTTGAAAACAAGAATTAAAACATTTCCCAAAACAATAGGCCAGCTTTATAACCTGCAAACTTTGAAAATACCTTATCAGCTTGAAGAGTTTCCAAAGGAAATTGCAAATTTGATCAACTTGCGGCACATTTATTTTGGTAGATATATGAAAGTTCCAAGTGGGATACTGGGACGATTCACTAATCTCCGGTCATTACCTTTTCTCAAGGTGGGTAAAGAGACAGGTCCGAGAATTGAAGAATTGAGTGGTTTAAACCAGTTGCACAACACCTTGTCTATTTATGGACTAGAAAATGTGGGAGATGGAGAAGAAGTGCAGAAAGCAAACTTAGTAGAGAAGAAACACATACGCAAGTTAATCCTTGGATGGAAGGTCAGTAGGCCAAGCCACAATGTGGAGAATGACGAAGATGTACTAGAAGGCCTCCGACCACATTCTAGATTGGAATTTTTGGAGATTCATGAATTCATGGGTGTTAAATTTCCGTCATGGTTATTGCTAGCCAACCATTTGAAAGAGATTGAATTATTGGGCTGCAACAAATGTGAAGGAGTCCCAGTACTGGGGCATTTACCCAATCTTAGTTGTGTTAAGATCAAGGAAATGGAGAATCTGACTTGTATAGGATCTGAGTTTTATGGTGATGACCATGTTAACTGTGGAAATGGATCAAGTAAGGAGGCACAGCCTTTGTTCCGTGCTTTGAAAACATTGCATATTGAGGATGCACCGAGCCTAATTGAATGGATGGAAGCGCCAACAGAGAGAGCAAGTAGGGTGTTTCCTTGCGTCGAGGAGCTGACCCTGTCAGGCTGTACACAATTGACAAGTGCTCCCAGTCATTTTCCATCTCTCAAGAGGTTGAAAATAGGAGGCATGAATAGCAGAGGCATGGCAATAGCAAGTATTCTAAGCAATCAACTCACCACTCTCACGCATCTCACGTTATCGGGTGTGAGTGGACTGACTTGTCTGCCGGAAGGGATGTTAGAAAACAACAAGAATCTTGCACATTTGGATATAGAGTTTTGTCCGGAATTAATTTGTATTTCTCCCCAATCACAAGGATCTGAGTACCGCTGTGCATCTCCTTCATATTTGCGGCTATTTTTTTGTGAGAATTTGAGATATTTACCTGATGGGATACTCACACCTTCTCTTAAAGAGCTGTCGTTGTTGCACTGTTATAATTTAGAGTACATCCCAGATGCTACAGACGGTAGTCTCACATCCCTTGAAACATTGAAGTCTACCGGGTGGGCTGGAATTTTGTACCTCTATTCGGAGTTTGACAATACAATTGTGCCCCAAGGTACCCTCCATTTCAATCGAAAGTCTGAGTACATCCCTCCAAGAGTTGTGTCATCATCACAGTCCGGCAGCCTTACTCGTTTGAAGAAATTGATAATTGGTGCTTTCTGCGAGGAGCTCGATGCATTCCCAGCTTTTCAGGCTATGCCACAGCTTGAATCATTACTCATCGGCGGGTGGCCTAAGCTCAAGTCTCTGCCTGAACAAATTCAACACTTGCCTTCTTTAAGAGATTTGGAGATAAGACATTTTGATGGAGTGGAGGCTATTCCAGAATGGTTGGGAAACCTTGCATCTCTTGAGGGCCTGACTTTTTATTGCTGCGAGAGTCTAAAGTATCTACCTTCTGTGGAAGCTATGCAACGCCTCACCAAATTGAAAGAGATATACATCTATGATTGTCCCCTTCTAGAAGAAAGATGCAGGGAGGAGAGCGGCCCAGAGTGGCCTAAGATTCGTCATCTTCCATTCATCAGGAAGTATAATAAATTTCGTAAAGTGACGACATCGAGGCAAATAAGGTTCCTATCTTTGAATGCTCTAAATCGGAAAATGCTTCAGTTACCTGGTGTTTCTTCAATGCTGCTGGTTGCCTCGAAGTATGAGGAGATCACACCTCCACATGTTGGAGAGTTGTGTTGCATAACAGATAAACATGAGTTTGATTTGCGCAATTCTACAATAAAGACGACTTTAAGGTTTGCAGGAGATCCAATAGAGTTGCTGAAGAGGGCGAAAAGGCTTTCCGAAACACCATATGAGGAGTGGTTGAGTCAGCTAAACTGGTAG

Protein Analysis

1267

Amino Acids

144.03

Weight (kDa)

6.89

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 99 7.3e-21 Rx N-terminal domain
nSTAND1 PF20703 166 - 391 5.3e-07 Novel STAND NTPase 1
NB-ARC PF00931 178 - 351 3.5e-35 NB-ARC domain
WHD_DRP PF23559 441 - 511 1.1e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 520 - 771 2.2e-25 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 646 - 770 8.7e-35 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 1073 - 1132 4.3e-07 R13L1/DRL21 LRRs
Cyclin_N PF00134 1179 - 1225 7.2e-08 Cyclin, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014222)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35910
fragaria_vesca FvH4_6g20630
malus_domestica MD12G1042400.v1.1 MD14G1041600.v1.1
prunus_persica Prupe.7G092500_v2.0.a1
pyrus_communis pycom14g03530
rosa_chinensis RchiOBHm_Chr3g0476521
rosa_laevigata RLG00000023765
rosa_multiflora Rmu_sc0004556.1_g000005 Rmu_sc0015190.1_g000003
rosa_roxburghii Rroxscaffold_6G00405260
rosa_rugosa Rorug03G0154500
rosa_samantha Rh3AG205600 Rh3BG238000 Rh3CG231800 Rh3DG231700
rosa_wichuraiana Rw3G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1752, 2892
AasI GACNNNNNNGTC 1 cut(s) 2673
Acc16I TGCGCA 1 cut(s) 3686
Acc36I ACCTGC 1 cut(s) 1764
Acc65I GGTACC 1 cut(s) 3017
AccB1I GGYRCC 1 cut(s) 3017
AccB7I CCANNNNNTGG 2 cut(s) 2098, 3641
AccBSI CCGCTC 1 cut(s) 3450
AccI GTMKAC 1 cut(s) 2954
AccII CGCG 1 cut(s) 174
AccIII TCCGGA 1 cut(s) 2737
AciI CCGC 8 cut(s) 174, 1119, 1550, 1825, 2782, 2806, 3189, 3450
AclWI GGATC 7 cut(s) 866, 1195, 1345, 2338, 2380, 2779, 3719
AcoI YGGCCR 1 cut(s) 412
AcuI CTGAAG 2 cut(s) 3557, 3761
AdeI CACNNNGTG 1 cut(s) 2098
AfaI GTAC 9 cut(s) 1128, 2118, 2265, 2521, 2780, 2903, 2978, 3019, 3047
AfiI CCNNNNNNNGG 8 cut(s) 629, 990, 1408, 2098, 2266, 2267, 2986, 3641
AflII CTTAAG 1 cut(s) 739
AflIII ACRYGT 3 cut(s) 1537, 1621, 3637
AjnI CCWGG 2 cut(s) 622, 3580
Alw21I GWGCWC 3 cut(s) 1069, 2539, 3135
Alw26I GTCTC 7 cut(s) 173, 493, 974, 1082, 1916, 2933, 3213
AlwI GGATC 7 cut(s) 866, 1195, 1345, 2338, 2380, 2779, 3719
Ama87I CYCGRG 1 cut(s) 924
Aor13HI TCCGGA 1 cut(s) 2737
AoxI GGCC 9 cut(s) 412, 992, 1741, 2084, 2125, 3194, 3319, 3451, 3461
ApeKI GCWGC 9 cut(s) 26, 62, 407, 933, 1159, 2238, 3084, 3336, 3598
AseI ATTAAT 2 cut(s) 1133, 2744
Asp700I GAANNNNTTC 3 cut(s) 1240, 1791, 3222
Asp718I GGTACC 1 cut(s) 3017
AspA2I CCTAGG 1 cut(s) 989
AspLEI GCGC 3 cut(s) 1164, 2461, 3687
AspS9I GGNCC 3 cut(s) 1928, 3319, 3452
AsuC2I CCSGG 1 cut(s) 2959
AsuHPI GGTGA 8 cut(s) 61, 208, 400, 1209, 1373, 2357, 2620, 3376
AsuNHI GCTAGC 1 cut(s) 2203
AvaI CYCGRG 1 cut(s) 924
AvaII GGWCC 1 cut(s) 1928
AvrII CCTAGG 1 cut(s) 989
BaeGI GKGCMC 1 cut(s) 3012
BanI GGYRCC 1 cut(s) 3017
BanII GRGCYC 1 cut(s) 3135
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 3 cut(s) 1069, 2539, 3135
BbvI GCAGC 9 cut(s) 13, 49, 419, 945, 1146, 2225, 3096, 3323, 3585
BccI CCATC 9 cut(s) 1288, 1357, 1402, 2000, 2064, 2446, 2560, 2837, 3263
BceAI ACGGC 1 cut(s) 399
BcgI CGANNNNNNTGC 2 cut(s) 1290, 1324
BciT130I CCWGG 2 cut(s) 624, 3582
BciVI GTATCC 3 cut(s) 721, 1860, 2841
BclI TGATCA 2 cut(s) 564, 1815
BcnI CCSGG 1 cut(s) 2959
BcoDI GTCTC 7 cut(s) 173, 493, 974, 1082, 1916, 2933, 3213
BfmI CTRYAG 1 cut(s) 2916
BfoI RGCGCY 1 cut(s) 2462
BfrI CTTAAG 1 cut(s) 739
BfuAI ACCTGC 1 cut(s) 1764
BfuI GTATCC 3 cut(s) 721, 1860, 2841
BglII AGATCT 3 cut(s) 100, 202, 735
BlnI CCTAGG 1 cut(s) 989
BmcAI AGTACT 1 cut(s) 2265
Bme1390I CCNGG 3 cut(s) 624, 2959, 3582
Bme18I GGWCC 1 cut(s) 1928
BmeT110I CYCGRG 1 cut(s) 924
BmgT120I GGNCC 3 cut(s) 1928, 3319, 3452
BmiI GGNNCC 2 cut(s) 3019, 3539
BmrFI CCNGG 3 cut(s) 624, 2959, 3582
BmrI ACTGGG 4 cut(s) 1880, 2255, 2276, 2535
BmtI GCTAGC 1 cut(s) 2207
BmuI ACTGGG 4 cut(s) 1880, 2255, 2276, 2535
BoxI GACNNNNGTC 1 cut(s) 2925
BpiI GAAGAC 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 3198
BpuMI CCSGG 1 cut(s) 2959
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 1622
BsaBI GATNNNNATC 1 cut(s) 3404
BsaI GGTCTC 1 cut(s) 974
BsaJI CCNNGG 5 cut(s) 30, 45, 989, 2065, 3013
BsaWI WCCGGW 3 cut(s) 598, 1890, 2737
BsaXI ACNNNNNCTCC 8 cut(s) 527, 557, 2001, 2031, 2247, 2277, 2521, 2551
Bsc4I CCNNNNNNNGG 8 cut(s) 629, 990, 1408, 2098, 2266, 2267, 2986, 3641
Bse1I ACTGG 7 cut(s) 1220, 1875, 1960, 2261, 2271, 2541, 3803
Bse3DI GCAATG 2 cut(s) 333, 2414
Bse8I GATNNNNATC 1 cut(s) 3404
BseAI TCCGGA 1 cut(s) 2737
BseBI CCWGG 2 cut(s) 624, 3582
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 5 cut(s) 30, 45, 989, 2065, 3013
BseJI GATNNNNATC 1 cut(s) 3404
BseLI CCNNNNNNNGG 8 cut(s) 629, 990, 1408, 2098, 2266, 2267, 2986, 3641
BseMI GCAATG 2 cut(s) 333, 2414
BseMII CTCAG 4 cut(s) 660, 2325, 2766, 3033
BseNI ACTGG 7 cut(s) 1220, 1875, 1960, 2261, 2271, 2541, 3803
BseRI GAGGAG 8 cut(s) 548, 910, 1427, 2513, 3143, 3458, 3635, 3791
BseSI GKGCMC 1 cut(s) 3012
BseXI GCAGC 9 cut(s) 13, 49, 419, 945, 1146, 2225, 3096, 3323, 3585
BseYI CCCAGC 1 cut(s) 3145
BsgI GTGCAG 1 cut(s) 2039
Bsh1236I CGCG 1 cut(s) 174
BshFI GGCC 9 cut(s) 414, 994, 1743, 2086, 2127, 3196, 3321, 3453, 3463
BshNI GGYRCC 1 cut(s) 3017
BshVI ATCGAT 1 cut(s) 507
BsiHKAI GWGCWC 3 cut(s) 1069, 2539, 3135
BsiHKCI CYCGRG 1 cut(s) 924
BsiSI CCGG 6 cut(s) 599, 1891, 2681, 2738, 2958, 3081
BslFI GGGAC 3 cut(s) 1887, 2243, 3402
BslI CCNNNNNNNGG 8 cut(s) 629, 990, 1408, 2098, 2266, 2267, 2986, 3641
BsmAI GTCTC 7 cut(s) 173, 493, 974, 1082, 1916, 2933, 3213
BsmBI CGTCTC 1 cut(s) 493
BsmFI GGGAC 3 cut(s) 1887, 2243, 3402
BsmI GAATGC 4 cut(s) 160, 624, 3140, 3556
BsnI GGCC 9 cut(s) 414, 994, 1743, 2086, 2127, 3196, 3321, 3453, 3463
Bso31I GGTCTC 1 cut(s) 974
BsoBI CYCGRG 1 cut(s) 924
Bsp1286I GDGCHC 4 cut(s) 1069, 2539, 3012, 3135
Bsp13I TCCGGA 1 cut(s) 2737
Bsp1407I TGTACA 1 cut(s) 2519
BspACI CCGC 8 cut(s) 174, 1119, 1550, 1825, 2782, 2806, 3189, 3450
BspANI GGCC 9 cut(s) 414, 994, 1743, 2086, 2127, 3196, 3321, 3453, 3463
BspCNI CTCAG 4 cut(s) 661, 2326, 2767, 3034
BspDI ATCGAT 1 cut(s) 507
BspEI TCCGGA 1 cut(s) 2737
BspFNI CGCG 1 cut(s) 174
BspHI TCATGA 2 cut(s) 661, 2164
BspLI GGNNCC 2 cut(s) 3019, 3539
BspMI ACCTGC 1 cut(s) 1764
BspOI GCTAGC 1 cut(s) 2207
BspPI GGATC 7 cut(s) 866, 1195, 1345, 2338, 2380, 2779, 3719
BspQI GCTCTTC 1 cut(s) 1322
BspT107I GGYRCC 1 cut(s) 3017
BspTI CTTAAG 1 cut(s) 739
BspTNI GGTCTC 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 3450
BsrDI GCAATG 2 cut(s) 333, 2414
BsrGI TGTACA 1 cut(s) 2519
BsrI ACTGG 7 cut(s) 1220, 1875, 1960, 2261, 2271, 2541, 3803
BssECI CCNNGG 5 cut(s) 30, 45, 989, 2065, 3013
BssT1I CCWWGG 4 cut(s) 45, 989, 2065, 3013
Bst2UI CCWGG 2 cut(s) 624, 3582
Bst4CI ACNGT 7 cut(s) 184, 487, 1202, 2363, 2888, 2924, 3078
Bst6I CTCTTC 3 cut(s) 1322, 1782, 3737
BstAFI CTTAAG 1 cut(s) 739
BstAUI TGTACA 1 cut(s) 2519
BstBAI YACGTR 1 cut(s) 1622
BstC8I GCNNGC 4 cut(s) 60, 412, 1745, 2205
BstFNI CGCG 1 cut(s) 174
BstH2I RGCGCY 1 cut(s) 2462
BstHHI GCGC 3 cut(s) 1164, 2461, 3687
BstMAI GTCTC 7 cut(s) 173, 493, 974, 1082, 1916, 2933, 3213
BstMWI GCNNNNNNNGC 4 cut(s) 224, 2586, 3146, 3155
BstNI CCWGG 2 cut(s) 624, 3582
BstNSI RCATGY 1 cut(s) 3641
BstPAI GACNNNNGTC 1 cut(s) 2925
BstSCI CCNGG 3 cut(s) 622, 2957, 3580
BstSFI CTRYAG 1 cut(s) 2916
BstSLI GKGCMC 1 cut(s) 3012
BstUI CGCG 1 cut(s) 174
BstV1I GCAGC 9 cut(s) 13, 49, 419, 945, 1146, 2225, 3096, 3323, 3585
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 7 cut(s) 100, 202, 735, 871, 2330, 2771, 3724
BstXI CCANNNNNNTGG 1 cut(s) 882
BstYI RGATCY 7 cut(s) 100, 202, 735, 871, 2330, 2771, 3724
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 3 cut(s) 721, 1860, 2841
BsuRI GGCC 9 cut(s) 414, 994, 1743, 2086, 2127, 3196, 3321, 3453, 3463
BsuTUI ATCGAT 1 cut(s) 507
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 474, 2884
BveI ACCTGC 1 cut(s) 1764
Cac8I GCNNGC 4 cut(s) 60, 412, 1745, 2205
CciI TCATGA 2 cut(s) 661, 2164
CfoI GCGC 3 cut(s) 1164, 2461, 3687
Cfr13I GGNCC 3 cut(s) 1928, 3319, 3452
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 2 cut(s) 276, 2482
CsiI ACCWGGT 1 cut(s) 3580
Csp6I GTAC 9 cut(s) 1127, 2117, 2264, 2520, 2779, 2902, 2977, 3018, 3046
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviQI GTAC 9 cut(s) 1127, 2117, 2264, 2520, 2779, 2902, 2977, 3018, 3046
DraI TTTAAA 3 cut(s) 349, 1435, 1956
DraIII CACNNNGTG 1 cut(s) 2098
DrdI GACNNNNNNGTC 1 cut(s) 2673
DseDI GACNNNNNNGTC 1 cut(s) 2673
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 3 cut(s) 1322, 1782, 3737
EarI CTCTTC 3 cut(s) 1322, 1782, 3737
Ecl136II GAGCTC 1 cut(s) 3133
Eco130I CCWWGG 4 cut(s) 45, 989, 2065, 3013
Eco147I AGGCCT 2 cut(s) 994, 2127
Eco24I GRGCYC 1 cut(s) 3135
Eco31I GGTCTC 1 cut(s) 974
Eco32I GATATC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 1928
Eco53kI GAGCTC 1 cut(s) 3133
Eco57I CTGAAG 2 cut(s) 3557, 3761
Eco88I CYCGRG 1 cut(s) 924
EcoICRI GAGCTC 1 cut(s) 3133
EcoO109I RGGNCCY 1 cut(s) 3319
EcoRI GAATTC 3 cut(s) 71, 1236, 2168
EcoRII CCWGG 2 cut(s) 622, 3580
EcoRV GATATC 1 cut(s) 505
EcoT14I CCWWGG 4 cut(s) 45, 989, 2065, 3013
EcoT22I ATGCAT 1 cut(s) 3142
EcoT38I GRGCYC 1 cut(s) 3135
ErhI CCWWGG 4 cut(s) 45, 989, 2065, 3013
Esp3I CGTCTC 1 cut(s) 493
FalI AAGNNNNNCTT 6 cut(s) 722, 754, 1331, 1363, 1362, 1394
FaqI GGGAC 3 cut(s) 1887, 2243, 3402
FauI CCCGC 3 cut(s) 167, 1543, 3182
FauNDI CATATG 1 cut(s) 3772
FbaI TGATCA 2 cut(s) 564, 1815
FblI GTMKAC 1 cut(s) 2954
FriOI GRGCYC 1 cut(s) 3135
FspI TGCGCA 1 cut(s) 3686
GlaI GCGC 3 cut(s) 1163, 2460, 3686
GsaI CCCAGC 1 cut(s) 3149
HaeII RGCGCY 1 cut(s) 2462
HaeIII GGCC 9 cut(s) 414, 994, 1743, 2086, 2127, 3196, 3321, 3453, 3463
HapII CCGG 6 cut(s) 599, 1891, 2681, 2738, 2958, 3081
HgaI GACGC 2 cut(s) 276, 2482
HhaI GCGC 3 cut(s) 1164, 2461, 3687
Hin6I GCGC 3 cut(s) 1162, 2459, 3685
HinP1I GCGC 3 cut(s) 1162, 2459, 3685
HincII GTYRAC 1 cut(s) 2359
HindII GTYRAC 1 cut(s) 2359
HpaI GTTAAC 1 cut(s) 2359
HpaII CCGG 6 cut(s) 599, 1891, 2681, 2738, 2958, 3081
HphI GGTGA 8 cut(s) 61, 208, 400, 1209, 1373, 2357, 2620, 3376
Hpy166II GTNNAC 6 cut(s) 1127, 1198, 2359, 2522, 2666, 2955
Hpy8I GTNNAC 6 cut(s) 1127, 1198, 2359, 2522, 2666, 2955
Hpy99I CGWCG 2 cut(s) 270, 2498
HpyCH4III ACNGT 7 cut(s) 184, 487, 1202, 2363, 2888, 2924, 3078
HpyCH4IV ACGT 4 cut(s) 1467, 1537, 1621, 2648
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 2586, 3146, 3155
HpySE526I ACGT 4 cut(s) 1467, 1537, 1621, 2648
HspAI GCGC 3 cut(s) 1162, 2459, 3685
Kpn2I TCCGGA 1 cut(s) 2737
KpnI GGTACC 1 cut(s) 3021
Ksp22I TGATCA 2 cut(s) 564, 1815
KspAI GTTAAC 1 cut(s) 2359
LguI GCTCTTC 1 cut(s) 1322
LmnI GCTCC 5 cut(s) 864, 1072, 2500, 2542, 3130
Lsp1109I GCAGC 9 cut(s) 13, 49, 419, 945, 1146, 2225, 3096, 3323, 3585
MabI ACCWGGT 1 cut(s) 3580
MaeII ACGT 4 cut(s) 1467, 1537, 1621, 2648
MbiI CCGCTC 1 cut(s) 3450
MfeI CAATTG 4 cut(s) 1191, 1668, 2525, 3003
MflI RGATCY 7 cut(s) 100, 202, 735, 871, 2330, 2771, 3724
MhlI GDGCHC 4 cut(s) 1069, 2539, 3012, 3135
MlyI GAGTC 4 cut(s) 113, 2265, 3352, 3796
MmeI TCCRAC 5 cut(s) 289, 516, 1378, 2155, 3622
Mph1103I ATGCAT 1 cut(s) 3142
MroI TCCGGA 1 cut(s) 2737
MroXI GAANNNNTTC 3 cut(s) 1240, 1791, 3222
MslI CAYNNNNRTG 1 cut(s) 3267
MspA1I CMGCKG 2 cut(s) 410, 2784
MspCI CTTAAG 1 cut(s) 739
MspI CCGG 6 cut(s) 599, 1891, 2681, 2738, 2958, 3081
MspR9I CCNGG 3 cut(s) 624, 2959, 3582
MssI GTTTAAAC 1 cut(s) 1956
MunI CAATTG 4 cut(s) 1191, 1668, 2525, 3003
Mva1269I GAATGC 4 cut(s) 160, 624, 3140, 3556
MvaI CCWGG 2 cut(s) 624, 3582
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 4 cut(s) 224, 2586, 3146, 3155
NciI CCSGG 1 cut(s) 2959
NdeI CATATG 1 cut(s) 3772
NheI GCTAGC 1 cut(s) 2203
NlaIV GGNNCC 2 cut(s) 3019, 3539
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 6 cut(s) 49, 116, 680, 943, 956, 3514
NsbI TGCGCA 1 cut(s) 3686
NsiI ATGCAT 1 cut(s) 3142
NspI RCATGY 1 cut(s) 3641
PaeR7I CTCGAG 1 cut(s) 924
PagI TCATGA 2 cut(s) 661, 2164
PceI AGGCCT 2 cut(s) 994, 2127
PciI ACATGT 1 cut(s) 3637
PciSI GCTCTTC 1 cut(s) 1322
PcsI WCGNNNNNNNCGW 1 cut(s) 2645
PctI GAATGC 4 cut(s) 160, 624, 3140, 3556
PdmI GAANNNNTTC 3 cut(s) 1240, 1791, 3222
PfeI GAWTC 8 cut(s) 133, 1214, 1878, 2161, 2314, 2707, 3173, 3469
PflFI GACNNNGTC 1 cut(s) 2509
PflMI CCANNNNNTGG 2 cut(s) 2098, 3641
PleI GAGTC 4 cut(s) 113, 2264, 3351, 3795
PmeI GTTTAAAC 1 cut(s) 1956
PpsI GAGTC 4 cut(s) 113, 2264, 3351, 3795
Ppu21I YACGTR 1 cut(s) 1622
PscI ACATGT 1 cut(s) 3637
PshAI GACNNNNGTC 1 cut(s) 2925
PshBI ATTAAT 2 cut(s) 1133, 2744
PsiI TTATAA 2 cut(s) 1752, 2892
Psp124BI GAGCTC 1 cut(s) 3135
Psp6I CCWGG 2 cut(s) 622, 3580
PspFI CCCAGC 1 cut(s) 3145
PspGI CCWGG 2 cut(s) 622, 3580
PspN4I GGNNCC 2 cut(s) 3019, 3539
PspPI GGNCC 3 cut(s) 1928, 3319, 3452
PspXI VCTCGAGB 1 cut(s) 924
PsuI RGATCY 7 cut(s) 100, 202, 735, 871, 2330, 2771, 3724
PsyI GACNNNGTC 1 cut(s) 2509
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 9 cut(s) 1128, 2118, 2265, 2521, 2780, 2903, 2978, 3019, 3047
RsaNI GTAC 9 cut(s) 1127, 2117, 2264, 2520, 2779, 2902, 2977, 3018, 3046
RseI CAYNNNNRTG 1 cut(s) 3267
SacI GAGCTC 1 cut(s) 3135
SapI GCTCTTC 1 cut(s) 1322
Sau96I GGNCC 3 cut(s) 1928, 3319, 3452
ScaI AGTACT 1 cut(s) 2265
SchI GAGTC 4 cut(s) 113, 2265, 3352, 3796
ScrFI CCNGG 3 cut(s) 624, 2959, 3582
SduI GDGCHC 4 cut(s) 1069, 2539, 3012, 3135
SexAI ACCWGGT 1 cut(s) 3580
SfcI CTRYAG 1 cut(s) 2916
Sfr274I CTCGAG 1 cut(s) 924
SinI GGWCC 1 cut(s) 1928
SlaI CTCGAG 1 cut(s) 924
SmiMI CAYNNNNRTG 1 cut(s) 3267
SseBI AGGCCT 2 cut(s) 994, 2127
SsiI CCGC 8 cut(s) 174, 1119, 1550, 1825, 2782, 2806, 3189, 3450
SspI AATATT 3 cut(s) 758, 1430, 1616
SstI GAGCTC 1 cut(s) 3135
StuI AGGCCT 2 cut(s) 994, 2127
StyD4I CCNGG 3 cut(s) 622, 2957, 3580
StyI CCWWGG 4 cut(s) 45, 989, 2065, 3013
TaaI ACNGT 7 cut(s) 184, 487, 1202, 2363, 2888, 2924, 3078
TaiI ACGT 4 cut(s) 1470, 1540, 1624, 2651
TatI WGTACW 5 cut(s) 2116, 2263, 2519, 2901, 3045
TauI GCSGC 3 cut(s) 1828, 2809, 3453
TfiI GAWTC 8 cut(s) 133, 1214, 1878, 2161, 2314, 2707, 3173, 3469
TscAI CASTG 2 cut(s) 474, 2891
TseFI GTSAC 6 cut(s) 49, 116, 680, 943, 956, 3514
TseI GCWGC 9 cut(s) 26, 62, 407, 933, 1159, 2238, 3084, 3336, 3598
Tsp45I GTSAC 6 cut(s) 49, 116, 680, 943, 956, 3514
TspGWI ACGGA 3 cut(s) 272, 2178, 2390
TspRI CASTG 2 cut(s) 474, 2891
Tth111I GACNNNGTC 1 cut(s) 2509
Van91I CCANNNNNTGG 2 cut(s) 2098, 3641
Vha464I CTTAAG 1 cut(s) 739
VpaK11BI GGWCC 1 cut(s) 1928
VspI ATTAAT 2 cut(s) 1133, 2744
XbaI TCTAGA 2 cut(s) 2141, 3424
XceI RCATGY 1 cut(s) 3641
XcmI CCANNNNNNNNNTGG 2 cut(s) 2360, 3777
XhoI CTCGAG 1 cut(s) 924
XmaJI CCTAGG 1 cut(s) 989
XmiI GTMKAC 1 cut(s) 2954
XmnI GAANNNNTTC 3 cut(s) 1240, 1791, 3222
ZrmI AGTACT 1 cut(s) 2265
Zsp2I ATGCAT 1 cut(s) 3142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.