FvH4_6g22720

TBCC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005065
Physical Location & Seq
Reverse (-)
16328327 .. 16336350
8024 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22720.t5

Sequence Viewer

Length: 1707 bp
ATGAGCGAGCCAATTGAGCCCTCAACGTCGTCGTCATCGGCCACCGCCGCCGCCAAAGACCCGGCCTTTCTGATTCATCCCCGGCGCGAGCCTTTCGAGCATGGGCTGCTCTCCATCCCCAAGCTCATCTTCGCCGACCCCACGCAAACCCTAATTCCCCTCAAGCACAAGCTCATCGAGCAGTCGCCGAATCACCGCGTCAACGCCGCCGCGATCTCCGAGTCGCTCCAGATCTCCATTGATCTCGCGAGGCTCGTCCTCGATACCCTAGCGTCGGTTCTTCACTTGGAATCGGACCCGCTCGTCCTGGCCAAGCCGGATGTGATTGATGAGGTCGGAGCCGATGTGCATGATCTGGTTCTGTTCCTCTACATTCAGTCCTACAAGAAATTGTTGCCGAGAACGCACAAGGATTCCGCCGCCGTTGCCGATGTTTGGCCGTCGACGTCAGCTTTCGACGGATATCTGTCGGCTCTGTCGCCGTTGCAGCTTGTGCGTAGCAATAGCCGTCGGTTTGTGCCATCACAGGCTGATGAAGAGGCCCATCAGTTATCATATCTGCAAAAGCATTTGGCTAACCTGATCTCACTGTTAGCAGAACCAGTGGAGGGGGAAGGCGAAGAATCTCTGGTTCTAACCATGGAAAGATTTGAGCACCTTGGGTTTCTGATCCAGTTTGGCGATAAAGGATCCGAAAGTGGTCAATTGAGCCAATATGCTCCCTTCTTTGCAAATTCAGATCCTGATATGCCTGCCGTTCCTGTCCCTGCCTCACAAGTTCATGAGTGGGTTTTAAGTAACATAGCTTCTGCTCTGGAAAATATTTCTGAAAGAATTTCTCCTAAAGAAAATGGCATGCCCAGTTCGTCAGATCAAGATGTTCCCATGGCTGATGCCTGCACAAGTTCTACCAAAGGCTCGACAAGTGTTAGAGGTTCAAGTTTTATTGAAGGGATCTCCAAATCCTCACTTGTAAGGCATGCATCTGATCTTAAAGGTCCTTCTGTAAAGGTTTTAAACTGCCACGATTCAGTGATCTATATATTAGCACCTCTGAGATATGCCACTGTGTATGGATGCTCTGATGCGACAATTGTCCTTGGAGCTGTTGGAAAGGCCGTAAGAATTGAGCACTGTGAGCGAGTTCATGTGATCACAGCAGCAAATCGAATCTGCATAGCTAACTGCCGCGAATGCATGTTTTTTCTCGGGGTAAACCAACGACCACTTATTGTTGGGGATAACCATAAGCTGCAGGTGGCACCATACAATACATTTTACAGTCAGTTGGGGGAGCACTTGTGTGAAGTCGGCATTGATGCTACTATCAATAGATGGGGTGAACCCCTGCCACTTGGAATGGTTGATCCACATGATTCACTATCTCATCCAGCAGGTGTCTCTGATGCTCAGGCTGAGTCTGCTAGTTGCTTAGACCCTGACCAGTTCACAAATTTTTTGATTCCTAACTGGTGTGGAGGTGAAGCACATGGCTCCACAAAGGACAACCCGTTTCCGTTACCAGAAGCTTATGTGGAATCTCAAGAAAGAAATAAGAATTTAGGGGAGGTAAAGCAATTATTGAGAGAAGCACCTCTTGAGGACAACAAAAAACGGGAACTATCGACTGCTCTTCACGTATATTTTAAAGACTGGTTGTATGCTTCTGGAAACATTCGACAGCTTTATTGCCTACAAGGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TBCC

Protein Analysis

569

Amino Acids

62.15

Weight (kDa)

5.26

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TBCC PF07986 335 - 378 2.6e-06 Tubulin binding cofactor C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1246, 1385
AasI GACNNNNNNGTC 1 cut(s) 302
AatII GACGTC 1 cut(s) 449
Acc36I ACCTGC 2 cut(s) 1246, 1385
AccB1I GGYRCC 1 cut(s) 1261
AccBSI CCGCTC 1 cut(s) 301
AccI GTMKAC 1 cut(s) 443
AccII CGCG 5 cut(s) 87, 198, 212, 248, 1191
AclWI GGATC 6 cut(s) 664, 684, 697, 734, 962, 1361
AcoI YGGCCR 3 cut(s) 39, 309, 437
AcsI RAATTY 4 cut(s) 733, 834, 1453, 1558
AcyI GRCGYC 1 cut(s) 446
AfiI CCNNNNNNNGG 3 cut(s) 274, 435, 608
AgsI TTSAA 2 cut(s) 939, 950
AjnI CCWGG 1 cut(s) 306
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AleI CACNNNNGTG 1 cut(s) 1302
Alw21I GWGCWC 3 cut(s) 657, 1134, 1299
Alw26I GTCTC 1 cut(s) 1405
AlwI GGATC 6 cut(s) 664, 684, 697, 734, 962, 1361
AlwNI CAGNNNCTG 1 cut(s) 743
Ama87I CYCGRG 1 cut(s) 1208
AoxI GGCC 6 cut(s) 39, 63, 309, 437, 540, 1116
ApeKI GCWGC 4 cut(s) 106, 487, 1160, 1252
ApoI RAATTY 4 cut(s) 733, 834, 1453, 1558
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 3 cut(s) 295, 541, 998
AsuC2I CCSGG 2 cut(s) 62, 82
AsuHPI GGTGA 3 cut(s) 185, 1352, 1493
AvaI CYCGRG 1 cut(s) 1208
AvaII GGWCC 2 cut(s) 295, 998
BalI TGGCCA 1 cut(s) 311
BamHI GGATCC 1 cut(s) 689
BanI GGYRCC 1 cut(s) 1261
BanII GRGCYC 1 cut(s) 21
BarI GAAGNNNNNNTAC 2 cut(s) 790, 822
Bbv12I GWGCWC 3 cut(s) 657, 1134, 1299
BbvI GCAGC 4 cut(s) 93, 499, 1172, 1239
BccI CCATC 4 cut(s) 122, 529, 552, 1329
BceAI ACGGC 6 cut(s) 407, 424, 466, 492, 740, 1103
BciT130I CCWGG 1 cut(s) 308
BclI TGATCA 1 cut(s) 1152
BcnI CCSGG 2 cut(s) 62, 82
BcoDI GTCTC 1 cut(s) 1405
BfaI CTAG 2 cut(s) 269, 1425
BfmI CTRYAG 1 cut(s) 1253
BfuAI ACCTGC 2 cut(s) 1246, 1385
BglII AGATCT 1 cut(s) 231
Bme1390I CCNGG 3 cut(s) 62, 82, 308
Bme18I GGWCC 2 cut(s) 295, 998
BmeT110I CYCGRG 1 cut(s) 1208
BmgT120I GGNCC 3 cut(s) 295, 541, 998
BmiI GGNNCC 5 cut(s) 297, 340, 691, 1263, 1495
BmrFI CCNGG 3 cut(s) 62, 82, 308
BmrI ACTGGG 1 cut(s) 855
BmsI GCATC 6 cut(s) 883, 992, 1067, 1075, 1309, 1396
BmuI ACTGGG 1 cut(s) 855
BoxI GACNNNNGTC 1 cut(s) 1094
BpmI CTGGAG 1 cut(s) 212
Bpu10I CCTNAGC 1 cut(s) 1410
BpuEI CTTGAG 3 cut(s) 146, 1527, 1619
BpuMI CCSGG 2 cut(s) 62, 82
BsaAI YACGTR 1 cut(s) 1639
BsaHI GRCGYC 1 cut(s) 446
BsaJI CCNNGG 5 cut(s) 80, 639, 658, 885, 1099
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 1286, 1316
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 435, 608
Bse1I ACTGG 6 cut(s) 602, 673, 861, 1444, 1475, 1658
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 5 cut(s) 80, 639, 658, 885, 1099
BseGI GGATG 5 cut(s) 76, 114, 325, 1082, 1387
BseLI CCNNNNNNNGG 3 cut(s) 274, 435, 608
BseMII CTCAG 3 cut(s) 1046, 1407, 1424
BseNI ACTGG 6 cut(s) 602, 673, 861, 1444, 1475, 1658
BseXI GCAGC 4 cut(s) 93, 499, 1172, 1239
BsgI GTGCAG 1 cut(s) 883
Bsh1236I CGCG 5 cut(s) 87, 198, 212, 248, 1191
BshFI GGCC 6 cut(s) 41, 65, 311, 439, 542, 1118
BshNI GGYRCC 1 cut(s) 1261
BsiHKAI GWGCWC 3 cut(s) 657, 1134, 1299
BsiHKCI CYCGRG 1 cut(s) 1208
BsiSI CCGG 3 cut(s) 62, 82, 317
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 3 cut(s) 274, 435, 608
BsmAI GTCTC 1 cut(s) 1405
BsmFI GGGAC 1 cut(s) 749
BsmI GAATGC 1 cut(s) 1199
BsnI GGCC 6 cut(s) 41, 65, 311, 439, 542, 1118
BsoBI CYCGRG 1 cut(s) 1208
Bsp1286I GDGCHC 4 cut(s) 21, 657, 1134, 1299
Bsp19I CCATGG 2 cut(s) 639, 885
Bsp68I TCGCGA 1 cut(s) 248
BspANI GGCC 6 cut(s) 41, 65, 311, 439, 542, 1118
BspCNI CTCAG 3 cut(s) 1047, 1408, 1423
BspFNI CGCG 5 cut(s) 87, 198, 212, 248, 1191
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 5 cut(s) 297, 340, 691, 1263, 1495
BspMAI CTGCAG 1 cut(s) 1257
BspMI ACCTGC 2 cut(s) 1246, 1385
BspPI GGATC 6 cut(s) 664, 684, 697, 734, 962, 1361
BspQI GCTCTTC 1 cut(s) 1638
BspT107I GGYRCC 1 cut(s) 1261
BsrBI CCGCTC 1 cut(s) 301
BsrI ACTGG 6 cut(s) 602, 673, 861, 1444, 1475, 1658
BssECI CCNNGG 5 cut(s) 80, 639, 658, 885, 1099
BssNI GRCGYC 1 cut(s) 446
BssT1I CCWWGG 4 cut(s) 639, 658, 885, 1099
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 4 cut(s) 591, 1069, 1136, 1283
Bst6I CTCTTC 2 cut(s) 531, 1638
BstACI GRCGYC 1 cut(s) 446
BstAPI GCANNNNNTGC 2 cut(s) 106, 493
BstBAI YACGTR 1 cut(s) 1639
BstC8I GCNNGC 6 cut(s) 8, 89, 753, 857, 898, 981
BstDEI CTNAG 4 cut(s) 1055, 1410, 1416, 1432
BstDSI CCRYGG 2 cut(s) 639, 885
BstF5I GGATG 5 cut(s) 76, 114, 325, 1082, 1387
BstFNI CGCG 5 cut(s) 87, 198, 212, 248, 1191
BstHHI GCGC 1 cut(s) 87
BstMAI GTCTC 1 cut(s) 1405
BstNI CCWGG 1 cut(s) 308
BstNSI RCATGY 3 cut(s) 859, 983, 1201
BstPAI GACNNNNGTC 1 cut(s) 1094
BstSCI CCNGG 3 cut(s) 60, 80, 306
BstSFI CTRYAG 1 cut(s) 1253
BstUI CGCG 5 cut(s) 87, 198, 212, 248, 1191
BstV1I GCAGC 4 cut(s) 93, 499, 1172, 1239
BstX2I RGATCY 4 cut(s) 231, 689, 739, 954
BstYI RGATCY 4 cut(s) 231, 689, 739, 954
BsuRI GGCC 6 cut(s) 41, 65, 311, 439, 542, 1118
BtgI CCRYGG 2 cut(s) 639, 885
BtsCI GGATG 5 cut(s) 76, 114, 325, 1082, 1387
BtsIMutI CAGTG 5 cut(s) 587, 609, 1038, 1065, 1132
BtuMI TCGCGA 1 cut(s) 248
BveI ACCTGC 2 cut(s) 1246, 1385
Cac8I GCNNGC 6 cut(s) 8, 89, 753, 857, 898, 981
CaiI CAGNNNCTG 1 cut(s) 743
CciI TCATGA 1 cut(s) 781
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 3 cut(s) 295, 541, 998
CseI GACGC 2 cut(s) 187, 261
DdeI CTNAG 4 cut(s) 1055, 1410, 1416, 1432
DraI TTTAAA 2 cut(s) 1017, 1648
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 3 cut(s) 39, 309, 437
Eam1104I CTCTTC 2 cut(s) 531, 1638
EarI CTCTTC 2 cut(s) 531, 1638
EciI GGCGGA 1 cut(s) 406
Eco130I CCWWGG 4 cut(s) 639, 658, 885, 1099
Eco24I GRGCYC 1 cut(s) 21
Eco32I GATATC 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 295, 998
Eco88I CYCGRG 1 cut(s) 1208
EcoO109I RGGNCCY 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 306
EcoRV GATATC 1 cut(s) 464
EcoT14I CCWWGG 4 cut(s) 639, 658, 885, 1099
EcoT22I ATGCAT 2 cut(s) 985, 1199
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 4 cut(s) 639, 658, 885, 1099
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 1581, 1613
FaqI GGGAC 1 cut(s) 749
FauI CCCGC 1 cut(s) 306
FbaI TGATCA 1 cut(s) 1152
FblI GTMKAC 1 cut(s) 443
FokI GGATG 5 cut(s) 63, 101, 332, 1089, 1374
FriOI GRGCYC 1 cut(s) 21
FspBI CTAG 2 cut(s) 269, 1425
GlaI GCGC 1 cut(s) 86
GsuI CTGGAG 1 cut(s) 212
HaeIII GGCC 6 cut(s) 41, 65, 311, 439, 542, 1118
HapII CCGG 3 cut(s) 62, 82, 317
HgaI GACGC 2 cut(s) 187, 261
HhaI GCGC 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 446
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 2 cut(s) 202, 444
HindII GTYRAC 2 cut(s) 202, 444
HindIII AAGCTT 1 cut(s) 1527
HpaII CCGG 3 cut(s) 62, 82, 317
HphI GGTGA 3 cut(s) 185, 1352, 1493
Hpy166II GTNNAC 5 cut(s) 202, 444, 1216, 1343, 1449
Hpy188III TCNNGA 9 cut(s) 229, 247, 743, 782, 815, 875, 1544, 1598, 1668
Hpy8I GTNNAC 5 cut(s) 202, 444, 1216, 1343, 1449
Hpy99I CGWCG 7 cut(s) 31, 34, 277, 445, 448, 461, 513
HpyAV CCTTC 4 cut(s) 608, 733, 944, 1011
HpyCH4III ACNGT 4 cut(s) 591, 1069, 1136, 1283
HpyCH4IV ACGT 3 cut(s) 26, 446, 1638
HpyCH4V TGCA 9 cut(s) 349, 487, 562, 731, 900, 983, 1176, 1197, 1255
HpyF3I CTNAG 4 cut(s) 1055, 1410, 1416, 1432
HpySE526I ACGT 3 cut(s) 26, 446, 1638
Hsp92I GRCGYC 1 cut(s) 446
HspAI GCGC 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 1152
LguI GCTCTTC 1 cut(s) 1638
LmnI GCTCC 6 cut(s) 231, 338, 724, 1103, 1294, 1499
Lsp1109I GCAGC 4 cut(s) 93, 499, 1172, 1239
LweI GCATC 6 cut(s) 883, 992, 1067, 1075, 1309, 1396
MaeI CTAG 2 cut(s) 269, 1425
MaeII ACGT 3 cut(s) 26, 446, 1638
MaeIII GTNAC 2 cut(s) 797, 1518
MbiI CCGCTC 1 cut(s) 301
MboII GAAGA 5 cut(s) 121, 272, 548, 632, 1625
MfeI CAATTG 3 cut(s) 12, 704, 1092
MflI RGATCY 4 cut(s) 231, 689, 739, 954
MhlI GDGCHC 4 cut(s) 21, 657, 1134, 1299
MlsI TGGCCA 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 2 cut(s) 230, 1427
MmeI TCCRAC 2 cut(s) 316, 1090
Mox20I TGGCCA 1 cut(s) 311
Mph1103I ATGCAT 2 cut(s) 985, 1199
MscI TGGCCA 1 cut(s) 311
MseI TTAA 4 cut(s) 794, 993, 1016, 1647
MslI CAYNNNNRTG 1 cut(s) 1302
Msp20I TGGCCA 1 cut(s) 311
MspI CCGG 3 cut(s) 62, 82, 317
MspR9I CCNGG 3 cut(s) 62, 82, 308
MunI CAATTG 3 cut(s) 12, 704, 1092
Mva1269I GAATGC 1 cut(s) 1199
MvaI CCWGG 1 cut(s) 308
MvnI CGCG 5 cut(s) 87, 198, 212, 248, 1191
NciI CCSGG 2 cut(s) 62, 82
NcoI CCATGG 2 cut(s) 639, 885
NlaIV GGNNCC 5 cut(s) 297, 340, 691, 1263, 1495
NmeAIII GCCGAG 1 cut(s) 423
NruI TCGCGA 1 cut(s) 248
NsiI ATGCAT 2 cut(s) 985, 1199
NspI RCATGY 3 cut(s) 859, 983, 1201
OliI CACNNNNGTG 1 cut(s) 1302
PaeI GCATGC 2 cut(s) 859, 983
PagI TCATGA 1 cut(s) 781
PaqCI CACCTGC 2 cut(s) 1246, 1385
PciSI GCTCTTC 1 cut(s) 1638
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 1 cut(s) 1199
PleI GAGTC 2 cut(s) 229, 1426
PpsI GAGTC 2 cut(s) 229, 1426
Ppu21I YACGTR 1 cut(s) 1639
PpuMI RGGWCCY 1 cut(s) 998
PshAI GACNNNNGTC 1 cut(s) 1094
Psp5II RGGWCCY 1 cut(s) 998
Psp6I CCWGG 1 cut(s) 306
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 5 cut(s) 297, 340, 691, 1263, 1495
PspPI GGNCC 3 cut(s) 295, 541, 998
PspPPI RGGWCCY 1 cut(s) 998
PstI CTGCAG 1 cut(s) 1257
PstNI CAGNNNCTG 1 cut(s) 743
PsuI RGATCY 4 cut(s) 231, 689, 739, 954
RruI TCGCGA 1 cut(s) 248
RseI CAYNNNNRTG 1 cut(s) 1302
SalI GTCGAC 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 1638
SaqAI TTAA 4 cut(s) 794, 993, 1016, 1647
Sau96I GGNCC 3 cut(s) 295, 541, 998
SchI GAGTC 2 cut(s) 230, 1427
ScrFI CCNGG 3 cut(s) 62, 82, 308
SduI GDGCHC 4 cut(s) 21, 657, 1134, 1299
SfaNI GCATC 6 cut(s) 883, 992, 1067, 1075, 1309, 1396
SfcI CTRYAG 1 cut(s) 1253
SgrDI CGTCGACG 1 cut(s) 442
SinI GGWCC 2 cut(s) 295, 998
SmiMI CAYNNNNRTG 1 cut(s) 1302
SmlI CTYRAG 3 cut(s) 161, 1542, 1598
SmoI CTYRAG 3 cut(s) 161, 1542, 1598
SphI GCATGC 2 cut(s) 859, 983
SspI AATATT 1 cut(s) 823
SspMI CTAG 2 cut(s) 269, 1425
StyD4I CCNGG 3 cut(s) 60, 80, 306
StyI CCWWGG 4 cut(s) 639, 658, 885, 1099
TaaI ACNGT 4 cut(s) 591, 1069, 1136, 1283
TaiI ACGT 3 cut(s) 29, 449, 1641
TaqI TCGA 9 cut(s) 96, 177, 261, 443, 456, 920, 1168, 1625, 1678
TauI GCSGC 6 cut(s) 50, 53, 209, 212, 422, 1191
Tru1I TTAA 4 cut(s) 794, 993, 1016, 1647
Tru9I TTAA 4 cut(s) 794, 993, 1016, 1647
TscAI CASTG 5 cut(s) 594, 609, 1038, 1072, 1139
TseI GCWGC 4 cut(s) 106, 487, 1160, 1252
TspDTI ATGAA 4 cut(s) 65, 549, 770, 1136
TspGWI ACGGA 2 cut(s) 474, 1506
TspRI CASTG 5 cut(s) 594, 609, 1038, 1072, 1139
VpaK11BI GGWCC 2 cut(s) 295, 998
XapI RAATTY 4 cut(s) 733, 834, 1453, 1558
XceI RCATGY 3 cut(s) 859, 983, 1201
XmiI GTMKAC 1 cut(s) 443
XspI CTAG 2 cut(s) 269, 1425
ZraI GACGTC 1 cut(s) 447
Zsp2I ATGCAT 2 cut(s) 985, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.