FvH4_6g24710

all-trans-retinol 13,14-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013428
Physical Location & Seq
Reverse (-)
18725210 .. 18725566
357 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24710.t1

Sequence Viewer

Length: 357 bp
ATGATCAAATCCGAGCTCCAGGGAACAACCAACCACATGGAGCTGTTGGAGAAGATGAATCTGAGAGTCGCCGAGGAATACAAAGGCTTCGGCGACGTGGCCTCCGGGCTCAGCGTCTTCGTGGAGCAGCTCAAGTCCAAAAGCGGCGGCTTCGACGAGTATGTCAAGCAGATTGATGCCATAGATCAGCAAGTCACCGAGTTCGAAGCTGTGGTTTCTGTGCTTGATAAACATGTGACCATGTTGGAGTCCAAATTAAGTACAGTCTGTGTATCACAATCCGTGGTCTTCCTAGTTTCAGTTGTTCGTGCTTGTTTCGTGTCTATAAAATTTGTGTTCTGTTTCTCTAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0016491 GO:0031082 GO:0031083 GO:0032991 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BLOC1_2

Protein Analysis

119

Amino Acids

13.2

Weight (kDa)

5.34

Isoelectric Point (pI)

31.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLOC1_2 PF10046 1 - 86 5.1e-26 Biogenesis of lysosome-related organelles complex-1 subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013428)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49550
fragaria_vesca FvH4_6g24710 FvH4_6g46100
malus_domestica MD09G1077100.v1.1 MD17G1067500.v1.1
prunus_persica Prupe.3G247000_v2.0.a1
pyrus_communis pycom17g06780
rosa_chinensis RchiOBHm_Chr2g0164421
rosa_laevigata RLG00000021460
rosa_multiflora Rmu_sc0039370.1_g000001
rosa_roxburghii Rroxscaffold_2G00086580
rosa_rugosa Rorug02G0508400
rosa_samantha Rh2AG576500 Rh2BG587500 Rh2CG558300 Rh2DG598100
rosa_wichuraiana Rw2G047750 Rw2G047850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AciI CCGC 2 cut(s) 144, 147
AcsI RAATTY 1 cut(s) 329
AfaI GTAC 2 cut(s) 262, 353
AflIII ACRYGT 1 cut(s) 232
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 4 cut(s) 16, 43, 130, 209
AluI AGCT 4 cut(s) 16, 43, 130, 209
Alw21I GWGCWC 1 cut(s) 18
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 187
AsuII TTCGAA 1 cut(s) 204
BanII GRGCYC 2 cut(s) 18, 111
BbsI GAAGAC 2 cut(s) 109, 280
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 139
BciT130I CCWGG 1 cut(s) 20
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 106
BfaI CTAG 1 cut(s) 293
BisI GCNGC 3 cut(s) 128, 145, 148
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 3 cut(s) 129, 146, 149
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 2 cut(s) 20, 106
BmgBI CACGTC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 20, 106
BmsI GCATC 1 cut(s) 166
BpiI GAAGAC 2 cut(s) 109, 280
Bpu1102I GCTNAGC 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 1 cut(s) 106
BsaJI CCNNGG 3 cut(s) 19, 72, 282
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 3 cut(s) 19, 72, 282
BseMII CTCAG 2 cut(s) 53, 124
BseXI GCAGC 1 cut(s) 139
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 1 cut(s) 105
BsnI GGCC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 18, 111
Bsp143I GATC 2 cut(s) 3, 184
Bsp1720I GCTNAGC 1 cut(s) 110
BspACI CCGC 2 cut(s) 144, 147
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 54, 123
BspT104I TTCGAA 1 cut(s) 204
BssECI CCNNGG 3 cut(s) 19, 72, 282
BssMI GATC 2 cut(s) 3, 184
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 204
BstDEI CTNAG 3 cut(s) 62, 110, 348
BstDSI CCRYGG 1 cut(s) 282
BstKTI GATC 2 cut(s) 6, 187
BstMBI GATC 2 cut(s) 3, 184
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 18, 104
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 2 cut(s) 109, 280
BstXI CCANNNNNNTGG 1 cut(s) 37
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 97
CseI GACGC 1 cut(s) 103
Csp6I GTAC 2 cut(s) 261, 352
CviAII CATG 3 cut(s) 37, 233, 241
CviJI RGCY 8 cut(s) 16, 43, 87, 101, 109, 130, 150, 209
CviKI_1 RGCY 8 cut(s) 16, 43, 87, 101, 109, 130, 150, 209
CviQI GTAC 2 cut(s) 261, 352
DdeI CTNAG 3 cut(s) 62, 110, 348
DpnI GATC 2 cut(s) 5, 186
DpnII GATC 2 cut(s) 3, 184
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 16
Eco24I GRGCYC 2 cut(s) 18, 111
Eco53kI GAGCTC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 18, 111
FaeI CATG 3 cut(s) 40, 236, 244
FaiI YATR 6 cut(s) 38, 162, 182, 234, 242, 326
FatI CATG 3 cut(s) 36, 232, 240
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 128, 145, 148
FriOI GRGCYC 2 cut(s) 18, 111
Fsp4HI GCNGC 3 cut(s) 128, 145, 148
FspBI CTAG 1 cut(s) 293
GluI GCNGC 3 cut(s) 128, 145, 148
HaeIII GGCC 1 cut(s) 101
HapII CCGG 1 cut(s) 105
HgaI GACGC 1 cut(s) 103
Hin1II CATG 3 cut(s) 40, 236, 244
HinfI GANTC 3 cut(s) 58, 66, 248
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 187
Hpy188I TCNGA 2 cut(s) 13, 63
Hpy99I CGWCG 2 cut(s) 98, 158
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 96
HpyF3I CTNAG 3 cut(s) 62, 110, 348
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 3 cut(s) 40, 236, 244
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 184
LmnI GCTCC 3 cut(s) 21, 40, 124
LpnPI CCDG 3 cut(s) 5, 32, 118
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 166
MaeI CTAG 1 cut(s) 293
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 193, 235
MalI GATC 2 cut(s) 5, 186
MboI GATC 2 cut(s) 3, 184
MboII GAAGA 3 cut(s) 64, 109, 280
MhlI GDGCHC 2 cut(s) 18, 111
MluCI AATT 2 cut(s) 254, 329
MlyI GAGTC 2 cut(s) 75, 257
MmeI TCCRAC 2 cut(s) 27, 225
MnlI CCTC 2 cut(s) 67, 112
MseI TTAA 1 cut(s) 257
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 2 cut(s) 20, 106
MvaI CCWGG 1 cut(s) 20
NciI CCSGG 1 cut(s) 106
NdeII GATC 2 cut(s) 3, 184
NlaIII CATG 3 cut(s) 40, 236, 244
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 2 cut(s) 193, 235
NspI RCATGY 1 cut(s) 236
NspV TTCGAA 1 cut(s) 204
PciI ACATGT 1 cut(s) 232
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 3 cut(s) 129, 146, 149
PleI GAGTC 2 cut(s) 74, 256
PpsI GAGTC 2 cut(s) 74, 256
PscI ACATGT 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
RsaI GTAC 2 cut(s) 262, 353
RsaNI GTAC 2 cut(s) 261, 352
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 3 cut(s) 128, 145, 148
Sau3AI GATC 2 cut(s) 3, 184
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 2 cut(s) 75, 257
ScrFI CCNGG 2 cut(s) 20, 106
SduI GDGCHC 2 cut(s) 18, 111
SetI ASST 5 cut(s) 18, 45, 99, 132, 211
SfaNI GCATC 1 cut(s) 166
SfuI TTCGAA 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 2 cut(s) 254, 329
SsiI CCGC 2 cut(s) 144, 147
SspMI CTAG 1 cut(s) 293
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 2 cut(s) 18, 104
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 153, 204
TasI AATT 2 cut(s) 254, 329
TatI WGTACW 2 cut(s) 260, 351
TauI GCSGC 2 cut(s) 147, 150
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TseFI GTSAC 2 cut(s) 193, 235
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 2 cut(s) 193, 235
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 1 cut(s) 271
XapI RAATTY 1 cut(s) 329
XceI RCATGY 1 cut(s) 236
XspI CTAG 1 cut(s) 293
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.