MD09G1077100.v1.1

Biogenesis of lysosome-related organelles complex 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013428
Physical Location & Seq
Reverse (-)
5428740 .. 5429108
369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1077100.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGCTGCTGCTGCGAAGGAAGAGCAAAAAGGCGAGCTCGCCGAGTCTATCAACGATCTCTTCGGTAGCGTCTCCGCCATGGTCAAATCGGAGCTCCAGGGATCGACCAACCACCTCGATCTCCTCGAAAAGATGAACCTGCGAGTCGCCGAAGAGTACAAGGGATTCGGCGACGTGGCGGCGGGGCTGAGCGTGTTCGTGGAGCAGCTCAAGGCCAAAAGCGGCAGCTTCGAGGAGTACGTGAAGCAGATCGACGTCATCGAGCAGCAGGTGACGGAGTTCGAGGCCGTGATTTCGGTGCTCGACAGATACGTGTCCATGTTGGAGTCCAAAGTCCAATCTGTTCATCAGAATCCTCCGCCTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0016491 GO:0031082 GO:0031083 GO:0032991 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BLOC1_2

Protein Analysis

123

Amino Acids

13.39

Weight (kDa)

4.63

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLOC1_2 PF10046 19 - 112 4.5e-29 Biogenesis of lysosome-related organelles complex-1 subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013428)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49550
fragaria_vesca FvH4_6g24710 FvH4_6g46100
malus_domestica MD09G1077100.v1.1 MD17G1067500.v1.1
prunus_persica Prupe.3G247000_v2.0.a1
pyrus_communis pycom17g06780
rosa_chinensis RchiOBHm_Chr2g0164421
rosa_laevigata RLG00000021460
rosa_multiflora Rmu_sc0039370.1_g000001
rosa_roxburghii Rroxscaffold_2G00086580
rosa_rugosa Rorug02G0508400
rosa_samantha Rh2AG576500 Rh2BG587500 Rh2CG558300 Rh2DG598100
rosa_wichuraiana Rw2G047750 Rw2G047850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 259
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 2 cut(s) 147, 259
AciI CCGC 5 cut(s) 75, 179, 182, 222, 359
AclWI GGATC 1 cut(s) 109
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 2 cut(s) 158, 239
AflIII ACRYGT 1 cut(s) 312
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 4 cut(s) 37, 94, 208, 228
AluI AGCT 4 cut(s) 37, 94, 208, 228
Alw21I GWGCWC 3 cut(s) 39, 96, 303
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 1 cut(s) 109
AoxI GGCC 2 cut(s) 213, 285
ApeKI GCWGC 6 cut(s) 5, 8, 11, 205, 225, 265
AsuHPI GGTGA 1 cut(s) 283
BanII GRGCYC 2 cut(s) 39, 96
Bbv12I GWGCWC 3 cut(s) 39, 96, 303
BbvI GCAGC 3 cut(s) 217, 237, 277
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 76
BfuAI ACCTGC 2 cut(s) 147, 259
BisI GCNGC 8 cut(s) 6, 9, 12, 180, 206, 223, 226, 266
BlpI GCTNAGC 1 cut(s) 188
BlsI GCNGC 8 cut(s) 7, 10, 13, 181, 207, 224, 227, 267
Bme1390I CCNGG 1 cut(s) 98
BmgBI CACGTC 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 80
Bpu1102I GCTNAGC 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 194
BsaAI YACGTR 2 cut(s) 241, 313
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 2 cut(s) 78, 97
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 78, 97
BseMII CTCAG 1 cut(s) 179
BseRI GAGGAG 2 cut(s) 113, 248
BseXI GCAGC 3 cut(s) 217, 237, 277
BshFI GGCC 2 cut(s) 215, 287
BsiHKAI GWGCWC 3 cut(s) 39, 96, 303
BsmAI GTCTC 1 cut(s) 76
BsmBI CGTCTC 1 cut(s) 76
BsnI GGCC 2 cut(s) 215, 287
Bsp1286I GDGCHC 3 cut(s) 39, 96, 303
Bsp143I GATC 4 cut(s) 55, 101, 118, 249
Bsp1720I GCTNAGC 1 cut(s) 188
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 5 cut(s) 75, 179, 182, 222, 359
BspANI GGCC 2 cut(s) 215, 287
BspCNI CTCAG 1 cut(s) 180
BspMI ACCTGC 2 cut(s) 147, 259
BspPI GGATC 1 cut(s) 109
BspQI GCTCTTC 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 78, 97
BssMI GATC 4 cut(s) 55, 101, 118, 249
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 98
Bst6I CTCTTC 3 cut(s) 15, 65, 147
BstACI GRCGYC 1 cut(s) 255
BstBAI YACGTR 2 cut(s) 241, 313
BstC8I GCNNGC 2 cut(s) 35, 39
BstDEI CTNAG 1 cut(s) 188
BstDSI CCRYGG 1 cut(s) 78
BstKTI GATC 4 cut(s) 58, 104, 121, 252
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 4 cut(s) 55, 101, 118, 249
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 217, 237, 277
BsuRI GGCC 2 cut(s) 215, 287
BtgI CCRYGG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 175
BveI ACCTGC 2 cut(s) 147, 259
Cac8I GCNNGC 2 cut(s) 35, 39
CseI GACGC 1 cut(s) 58
Csp6I GTAC 2 cut(s) 157, 238
CviAII CATG 2 cut(s) 79, 319
CviJI RGCY 8 cut(s) 5, 37, 94, 187, 208, 215, 228, 287
CviKI_1 RGCY 8 cut(s) 5, 37, 94, 187, 208, 215, 228, 287
CviQI GTAC 2 cut(s) 157, 238
DdeI CTNAG 1 cut(s) 188
DpnI GATC 4 cut(s) 57, 103, 120, 251
DpnII GATC 4 cut(s) 55, 101, 118, 249
Eam1104I CTCTTC 3 cut(s) 15, 65, 147
EarI CTCTTC 3 cut(s) 15, 65, 147
EciI GGCGGA 2 cut(s) 64, 348
Ecl136II GAGCTC 2 cut(s) 37, 94
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 39, 96
Eco53kI GAGCTC 2 cut(s) 37, 94
EcoICRI GAGCTC 2 cut(s) 37, 94
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 2 cut(s) 39, 96
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 2 cut(s) 82, 322
FaiI YATR 2 cut(s) 80, 320
FatI CATG 2 cut(s) 78, 318
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 8 cut(s) 6, 9, 12, 180, 206, 223, 226, 266
FriOI GRGCYC 2 cut(s) 39, 96
Fsp4HI GCNGC 8 cut(s) 6, 9, 12, 180, 206, 223, 226, 266
GluI GCNGC 8 cut(s) 6, 9, 12, 180, 206, 223, 226, 266
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 2 cut(s) 215, 287
HgaI GACGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 2 cut(s) 82, 322
HinfI GANTC 5 cut(s) 44, 144, 165, 326, 352
HphI GGTGA 1 cut(s) 283
Hpy188I TCNGA 2 cut(s) 91, 351
Hpy188III TCNNGA 1 cut(s) 366
Hpy99I CGWCG 2 cut(s) 176, 257
HpyAV CCTTC 1 cut(s) 10
HpyCH4IV ACGT 4 cut(s) 174, 240, 255, 312
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 4 cut(s) 174, 240, 255, 312
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 2 cut(s) 82, 322
Kzo9I GATC 4 cut(s) 55, 101, 118, 249
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 3 cut(s) 91, 99, 202
LpnPI CCDG 4 cut(s) 83, 110, 152, 254
Lsp1109I GCAGC 3 cut(s) 217, 237, 277
MaeII ACGT 4 cut(s) 174, 240, 255, 312
MaeIII GTNAC 1 cut(s) 271
MalI GATC 4 cut(s) 57, 103, 120, 251
MboI GATC 4 cut(s) 55, 101, 118, 249
MboII GAAGA 3 cut(s) 32, 52, 164
MhlI GDGCHC 3 cut(s) 39, 96, 303
MlyI GAGTC 3 cut(s) 53, 153, 335
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 5 cut(s) 125, 134, 226, 277, 366
MspR9I CCNGG 1 cut(s) 98
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 1 cut(s) 11
NcoI CCATGG 1 cut(s) 78
NdeII GATC 4 cut(s) 55, 101, 118, 249
NlaIII CATG 2 cut(s) 82, 322
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 15
PcsI WCGNNNNNNNCGW 4 cut(s) 123, 237, 258, 309
PfeI GAWTC 2 cut(s) 165, 352
PkrI GCNGC 8 cut(s) 7, 10, 13, 181, 207, 224, 227, 267
PleI GAGTC 3 cut(s) 52, 152, 334
PpsI GAGTC 3 cut(s) 52, 152, 334
Ppu21I YACGTR 2 cut(s) 241, 313
Psp124BI GAGCTC 2 cut(s) 39, 96
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
RsaI GTAC 2 cut(s) 158, 239
RsaNI GTAC 2 cut(s) 157, 238
SacI GAGCTC 2 cut(s) 39, 96
SapI GCTCTTC 1 cut(s) 15
SatI GCNGC 8 cut(s) 6, 9, 12, 180, 206, 223, 226, 266
Sau3AI GATC 4 cut(s) 55, 101, 118, 249
SchI GAGTC 3 cut(s) 53, 153, 335
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 3 cut(s) 39, 96, 303
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SsiI CCGC 5 cut(s) 75, 179, 182, 222, 359
SstI GAGCTC 2 cut(s) 39, 96
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 78
TaiI ACGT 4 cut(s) 177, 243, 258, 315
TaqI TCGA 8 cut(s) 104, 117, 126, 231, 252, 261, 282, 303
TatI WGTACW 1 cut(s) 156
TauI GCSGC 2 cut(s) 182, 225
TfiI GAWTC 2 cut(s) 165, 352
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 6 cut(s) 5, 8, 11, 205, 225, 265
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 2 cut(s) 149, 335
TspGWI ACGGA 1 cut(s) 290
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.