Rroxscaffold_2G00086580

Biogenesis of lysosome-related organelles complex 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013428
Physical Location & Seq
Reverse (-)
8758798 .. 8759430
633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086580.1

Sequence Viewer

Length: 351 bp
ATGGCTGCAAAAGACGAGCTCGCCGAGTCTCTCAATGACCTCTTCACCAGCGTCTCCACCATGGTCAAATCGGAGCTCCAGGGAACGACCAACCACATGGAGCTCTTAGAGAAGATGAATCTGAGAGTCGCCGAGGAATACAAAGGCTTCGGCGACGTGGCCTCCGGGCTAAGCGTTTTCGTGGAGCAGCTCAAGTCCAAAAGCGGCGGCTTCGACGAGTATGTGAAGCAGATTGATGTCATAGACCAGCAAGTCACCGAATTCGAAGCTGTGGTTTCAGTGCTTGATCGATATGTGGCCATGTTAGAGTCCAAAGTCCAATCCGTGTATCAAAATCCATCGTCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0016491 GO:0031082 GO:0031083 GO:0032991 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BLOC1_2

Protein Analysis

116

Amino Acids

12.87

Weight (kDa)

4.46

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLOC1_2 PF10046 13 - 106 8.1e-30 Biogenesis of lysosome-related organelles complex-1 subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013428)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49550
fragaria_vesca FvH4_6g24710 FvH4_6g46100
malus_domestica MD09G1077100.v1.1 MD17G1067500.v1.1
prunus_persica Prupe.3G247000_v2.0.a1
pyrus_communis pycom17g06780
rosa_chinensis RchiOBHm_Chr2g0164421
rosa_laevigata RLG00000021460
rosa_multiflora Rmu_sc0039370.1_g000001
rosa_roxburghii Rroxscaffold_2G00086580
rosa_rugosa Rorug02G0508400
rosa_samantha Rh2AG576500 Rh2BG587500 Rh2CG558300 Rh2DG598100
rosa_wichuraiana Rw2G047750 Rw2G047850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AciI CCGC 2 cut(s) 204, 207
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 1 cut(s) 260
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 5 cut(s) 19, 76, 103, 190, 269
AluI AGCT 5 cut(s) 19, 76, 103, 190, 269
Alw21I GWGCWC 3 cut(s) 21, 78, 105
Alw26I GTCTC 2 cut(s) 33, 58
AoxI GGCC 2 cut(s) 159, 297
ApeKI GCWGC 2 cut(s) 5, 187
ApoI RAATTY 1 cut(s) 260
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 37, 247
AsuII TTCGAA 1 cut(s) 264
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 3 cut(s) 21, 78, 105
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 3 cut(s) 21, 78, 105
BbvI GCAGC 1 cut(s) 199
BccI CCATC 1 cut(s) 346
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 33, 58
BisI GCNGC 4 cut(s) 6, 188, 205, 208
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 4 cut(s) 7, 189, 206, 209
Bme1390I CCNGG 2 cut(s) 80, 166
BmgBI CACGTC 1 cut(s) 157
BmrFI CCNGG 2 cut(s) 80, 166
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 289
BsaJI CCNNGG 3 cut(s) 60, 79, 132
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
BseBI CCWGG 1 cut(s) 80
BseCI ATCGAT 1 cut(s) 289
BseDI CCNNGG 3 cut(s) 60, 79, 132
BseMII CTCAG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 199
BshFI GGCC 2 cut(s) 161, 299
BshVI ATCGAT 1 cut(s) 289
BsiHKAI GWGCWC 3 cut(s) 21, 78, 105
BsiSI CCGG 1 cut(s) 165
BsmAI GTCTC 2 cut(s) 33, 58
BsmBI CGTCTC 1 cut(s) 58
BsnI GGCC 2 cut(s) 161, 299
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 3 cut(s) 21, 78, 105
Bsp143I GATC 1 cut(s) 286
Bsp1720I GCTNAGC 1 cut(s) 170
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 2 cut(s) 204, 207
BspANI GGCC 2 cut(s) 161, 299
BspCNI CTCAG 1 cut(s) 114
BspDI ATCGAT 1 cut(s) 289
BspT104I TTCGAA 1 cut(s) 264
BssECI CCNNGG 3 cut(s) 60, 79, 132
BssMI GATC 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 4 cut(s) 106, 122, 170, 348
BstDSI CCRYGG 1 cut(s) 60
BstKTI GATC 1 cut(s) 289
BstMAI GTCTC 2 cut(s) 33, 58
BstMBI GATC 1 cut(s) 286
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 78, 164
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 1 cut(s) 336
BstXI CCANNNNNNTGG 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 289
BsuRI GGCC 2 cut(s) 161, 299
BsuTUI ATCGAT 1 cut(s) 289
BtgI CCRYGG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 21
ClaI ATCGAT 1 cut(s) 289
CseI GACGC 1 cut(s) 40
CviAII CATG 3 cut(s) 61, 97, 301
DdeI CTNAG 4 cut(s) 106, 122, 170, 348
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Ecl136II GAGCTC 3 cut(s) 19, 76, 103
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 3 cut(s) 21, 78, 105
Eco53kI GAGCTC 3 cut(s) 19, 76, 103
EcoICRI GAGCTC 3 cut(s) 19, 76, 103
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 3 cut(s) 21, 78, 105
ErhI CCWWGG 1 cut(s) 60
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 3 cut(s) 64, 100, 304
FaiI YATR 6 cut(s) 62, 98, 222, 242, 294, 302
FatI CATG 3 cut(s) 60, 96, 300
Fnu4HI GCNGC 4 cut(s) 6, 188, 205, 208
FriOI GRGCYC 3 cut(s) 21, 78, 105
Fsp4HI GCNGC 4 cut(s) 6, 188, 205, 208
GluI GCNGC 4 cut(s) 6, 188, 205, 208
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 2 cut(s) 161, 299
HapII CCGG 1 cut(s) 165
HgaI GACGC 1 cut(s) 40
Hin1II CATG 3 cut(s) 64, 100, 304
HinfI GANTC 4 cut(s) 26, 118, 126, 308
HpaII CCGG 1 cut(s) 165
HphI GGTGA 2 cut(s) 37, 247
Hpy188I TCNGA 2 cut(s) 73, 123
Hpy99I CGWCG 2 cut(s) 158, 218
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 1 cut(s) 8
HpyF3I CTNAG 4 cut(s) 106, 122, 170, 348
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 3 cut(s) 64, 100, 304
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 4 cut(s) 73, 81, 100, 184
LpnPI CCDG 5 cut(s) 61, 65, 92, 178, 260
Lsp1109I GCAGC 1 cut(s) 199
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 253
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 3 cut(s) 34, 124, 336
MhlI GDGCHC 3 cut(s) 21, 78, 105
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 1 cut(s) 260
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 3 cut(s) 35, 135, 317
MnlI CCTC 3 cut(s) 50, 127, 172
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 2 cut(s) 80, 166
MvaI CCWGG 1 cut(s) 80
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 60
NdeII GATC 1 cut(s) 286
NlaIII CATG 3 cut(s) 64, 100, 304
NmeAIII GCCGAG 2 cut(s) 49, 157
NmuCI GTSAC 1 cut(s) 253
NspV TTCGAA 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PfeI GAWTC 1 cut(s) 118
PkrI GCNGC 4 cut(s) 7, 189, 206, 209
PleI GAGTC 3 cut(s) 34, 134, 316
PpsI GAGTC 3 cut(s) 34, 134, 316
Psp124BI GAGCTC 3 cut(s) 21, 78, 105
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
SacI GAGCTC 3 cut(s) 21, 78, 105
SatI GCNGC 4 cut(s) 6, 188, 205, 208
Sau3AI GATC 1 cut(s) 286
SchI GAGTC 3 cut(s) 35, 135, 317
ScrFI CCNGG 2 cut(s) 80, 166
SduI GDGCHC 3 cut(s) 21, 78, 105
SetI ASST 7 cut(s) 21, 42, 78, 105, 159, 192, 271
SfuI TTCGAA 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 1 cut(s) 260
SsiI CCGC 2 cut(s) 204, 207
SstI GAGCTC 3 cut(s) 21, 78, 105
StyD4I CCNGG 2 cut(s) 78, 164
StyI CCWWGG 1 cut(s) 60
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 213, 264, 289
TasI AATT 1 cut(s) 260
TauI GCSGC 2 cut(s) 207, 210
TfiI GAWTC 1 cut(s) 118
TscAI CASTG 1 cut(s) 285
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 2 cut(s) 5, 187
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 131
TspGWI ACGGA 1 cut(s) 313
TspRI CASTG 1 cut(s) 285
XapI RAATTY 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.