FvH4_6g25130

Lycopene beta cyclase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007985
Physical Location & Seq
Forward (+)
19067251 .. 19069369
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g25130.t3

Sequence Viewer

Length: 1494 bp
ATGGATACATTACTGAAAACACATAACAAGCTCGAGTTTCTGCACCCACTTCATGGGCTTTCAGAGAAAGTGAGTAATTGGAACTCTTCAAAGCAGGAGCTTAGATGTGGTCTCAGGAAGTCCTGTAGAACTGGCCGTGTTAAGGCCAGAAGTAGTGCTCTTCTGGAGCTGGTGCCTGAAACCAAGAAGGAGAATCTCGAATTTGAGCTTCCCTTGTATGACCCATCAAAGGGTCTTGTGGTGGACCTTGCAGTCGTGGGAGGCGGCCCTGCAGGGCTTGCCGTGGCACAGCAAGTCTCCGAGGCAGGGTTATCTGTTTGCTCGATTGATCCCTCTCCCAAGTTAATCTGGCCCAACAATTACGGTGTTTGGGTGGATGAGTTTGAGGCCATGGATTTGCTTGATTGCCTCGACACTACCTGGTCTGGTGCTGTTGTGTTCATAGATGAACATTCGAAGAAGGATCTTGATAGGCCTTATGGGAGGGTTAATAGGAAGCAGCTCAAGTCCAAAATGCTGCAGAAATGCATTTCAAATGGTGTCAAGTTTCATCAAGCTAAAGTCAATAACGTTATTCACGAGGAGGACAAATCACTCTTGGTTTGCAGTGATGGCGTCACAATTCAAGCTACTGTGGTTCTTGATGCAACAGGCTTTTCAAGATGCCTTGTGCAGTATGATAAGCCTTACAATCCAGGTTACCAAGTGGCGTATGGGATTTTGGCAGAAGTTGAAGAACATCCTTTTGATGTGGATAAGATGGTTTTCATGGATTGGAGAGATTCACATTTGAGGAATAGTCCTCAACTGAAAGAGAGAAATAGTAAGATCCCTACTTTTCTATATGCAATGCCTTTTTCATCCAATAGGATATTTCTTGAAGAAACGTCCCTAGTTGCTCGGCCAGGACTACCCATGAAAGATATCCAGGAAAGGATGGTTGCTAGATTGAAACACCTAGGCATAAAAGTTAAGAGCATCGAAGAGGATGAGCATTGTGTCATTCCGATGGGCGGGCCTCTACCGGTGCTCCCTCAAAGAGTTGTTGGAATTGGAGGTACAGCTGGGATGGTGCACCCTTCAACAGGGTATATGGTCGCGAGGACTCTGGCAGCAGCTCCTATTGTTGCAAATGCAATAGTTCAGTACCTTGGTTCTGGTAGAAGTATTTCAGGAAATGAACTGTCAGCAGAAGTGTGGAAAGATCTATGGCCCATACAAAGGAGGAGACAAAGGGAGTTCTTCTGTTTTGGCATGTCCATTCTGCTCAAGCTGGATTTGAATGCCACAAGAAGGTTTTTCAATGCATTTTTTGATCTAGAACCTCGTTATTGGCATGGATTCTTATCATCTCGACTGTTTCTTCCTGATCTTCTTTTCTTCGGCCTTTCACTGTTTTCTCATGCTTCAAATGCTAGTAGGCTAGAAATAATGGGAAAGGGTACTATTCCTTTGGTAAAGATGATCAACAACTTGATACAGGATAGAGATTAG

Protein Analysis

498

Amino Acids

55.83

Weight (kDa)

8.44

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lycopene_cycl PF05834 82 - 474 7.7e-150 Lycopene cyclase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 172
AccB7I CCANNNNNTGG 1 cut(s) 53
AccII CGCG 1 cut(s) 1100
AciI CCGC 2 cut(s) 264, 1014
AclI AACGTT 1 cut(s) 570
AclWI GGATC 3 cut(s) 323, 471, 823
AcoI YGGCCR 2 cut(s) 133, 902
AcsI RAATTY 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 615
AfaI GTAC 3 cut(s) 1060, 1148, 1444
AfiI CCNNNNNNNGG 9 cut(s) 53, 142, 229, 230, 306, 1013, 1085, 1221, 1293
AgeI ACCGGT 1 cut(s) 1024
AjnI CCWGG 4 cut(s) 419, 694, 904, 927
Alw21I GWGCWC 3 cut(s) 160, 1032, 1077
Alw26I GTCTC 3 cut(s) 116, 301, 1222
Alw44I GTGCAC 1 cut(s) 1073
AlwI GGATC 3 cut(s) 323, 471, 823
Ama87I CYCGRG 1 cut(s) 32
ApaLI GTGCAC 1 cut(s) 1073
ApeKI GCWGC 4 cut(s) 499, 517, 1112, 1115
ApoI RAATTY 1 cut(s) 200
AsiGI ACCGGT 1 cut(s) 1024
Asp700I GAANNNNTTC 1 cut(s) 1168
AspA2I CCTAGG 1 cut(s) 958
AspS9I GGNCC 5 cut(s) 244, 266, 351, 1016, 1212
AsuII TTCGAA 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 1 cut(s) 244
AvrII CCTAGG 1 cut(s) 958
BaeGI GKGCMC 1 cut(s) 1077
BanI GGYRCC 1 cut(s) 172
BauI CACGAG 1 cut(s) 578
Bbv12I GWGCWC 3 cut(s) 160, 1032, 1077
BbvI GCAGC 4 cut(s) 504, 511, 1124, 1127
BccI CCATC 6 cut(s) 232, 605, 754, 931, 1003, 1063
BceAI ACGGC 2 cut(s) 120, 266
BciT130I CCWGG 4 cut(s) 421, 696, 906, 929
BclI TGATCA 1 cut(s) 1464
BcoDI GTCTC 3 cut(s) 116, 301, 1222
BfaI CTAG 6 cut(s) 893, 945, 959, 1319, 1416, 1424
BfmI CTRYAG 3 cut(s) 124, 270, 518
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
BlnI CCTAGG 1 cut(s) 958
BlsI GCNGC 5 cut(s) 266, 501, 519, 1114, 1117
Bme1390I CCNGG 4 cut(s) 421, 696, 906, 929
Bme18I GGWCC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 5 cut(s) 244, 266, 351, 1016, 1212
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 4 cut(s) 421, 696, 906, 929
BmsI GCATC 3 cut(s) 634, 653, 987
BpmI CTGGAG 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 455
BpuEI CTTGAG 2 cut(s) 488, 1253
BsaBI GATNNNNATC 1 cut(s) 1345
BsaHI GRCGYC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 5 cut(s) 282, 300, 390, 958, 1150
BsaWI WCCGGW 1 cut(s) 1024
BsaXI ACNNNNNCTCC 2 cut(s) 1014, 1044
Bsc4I CCNNNNNNNGG 9 cut(s) 53, 142, 229, 230, 306, 1013, 1085, 1221, 1293
Bse118I RCCGGY 1 cut(s) 1024
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 855
Bse8I GATNNNNATC 1 cut(s) 1345
BseBI CCWGG 4 cut(s) 421, 696, 906, 929
BseDI CCNNGG 5 cut(s) 282, 300, 390, 958, 1150
BseGI GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BseJI GATNNNNATC 1 cut(s) 1345
BseLI CCNNNNNNNGG 9 cut(s) 53, 142, 229, 230, 306, 1013, 1085, 1221, 1293
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 596, 1240
BseSI GKGCMC 1 cut(s) 1077
BseXI GCAGC 4 cut(s) 504, 511, 1124, 1127
BseYI CCCAGC 1 cut(s) 1064
BsgI GTGCAG 2 cut(s) 26, 692
Bsh1236I CGCG 1 cut(s) 1100
BshNI GGYRCC 1 cut(s) 172
BshTI ACCGGT 1 cut(s) 1024
BsiHKAI GWGCWC 3 cut(s) 160, 1032, 1077
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 1 cut(s) 1025
BslFI GGGAC 1 cut(s) 874
BslI CCNNNNNNNGG 9 cut(s) 53, 142, 229, 230, 306, 1013, 1085, 1221, 1293
BsmAI GTCTC 3 cut(s) 116, 301, 1222
BsmFI GGGAC 1 cut(s) 874
BsmI GAATGC 1 cut(s) 1288
Bso31I GGTCTC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 32
Bsp119I TTCGAA 1 cut(s) 455
Bsp1286I GDGCHC 3 cut(s) 160, 1032, 1077
Bsp143I GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
Bsp19I CCATGG 1 cut(s) 390
Bsp68I TCGCGA 1 cut(s) 1100
BspACI CCGC 2 cut(s) 264, 1014
BspCNI CTCAG 1 cut(s) 126
BspFNI CGCG 1 cut(s) 1100
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 2 cut(s) 274, 522
BspPI GGATC 3 cut(s) 323, 471, 823
BspQI GCTCTTC 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 455
BspT107I GGYRCC 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 855
BsrFI RCCGGY 1 cut(s) 1024
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 1024
BssECI CCNNGG 5 cut(s) 282, 300, 390, 958, 1150
BssMI GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
BssNI GRCGYC 1 cut(s) 615
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 3 cut(s) 390, 958, 1150
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 4 cut(s) 421, 696, 906, 929
Bst4CI ACNGT 5 cut(s) 365, 634, 1185, 1359, 1395
Bst6I CTCTTC 3 cut(s) 91, 165, 978
BstACI GRCGYC 1 cut(s) 615
BstAPI GCANNNNNTGC 1 cut(s) 278
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 279, 1016
BstDEI CTNAG 2 cut(s) 101, 113
BstDSI CCRYGG 2 cut(s) 282, 390
BstEII GGTNACC 1 cut(s) 698
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BstFNI CGCG 1 cut(s) 1100
BstKTI GATC 7 cut(s) 331, 466, 831, 1207, 1318, 1372, 1467
BstMAI GTCTC 3 cut(s) 116, 301, 1222
BstMBI GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
BstMWI GCNNNNNNNGC 3 cut(s) 278, 612, 1412
BstNI CCWGG 4 cut(s) 421, 696, 906, 929
BstNSI RCATGY 1 cut(s) 1258
BstPI GGTNACC 1 cut(s) 698
BstSCI CCNGG 4 cut(s) 419, 694, 904, 927
BstSFI CTRYAG 3 cut(s) 124, 270, 518
BstSLI GKGCMC 1 cut(s) 1077
BstUI CGCG 1 cut(s) 1100
BstV1I GCAGC 4 cut(s) 504, 511, 1124, 1127
BstX2I RGATCY 3 cut(s) 463, 828, 1204
BstYI RGATCY 3 cut(s) 463, 828, 1204
BtgI CCRYGG 2 cut(s) 282, 390
BtsCI GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 613, 1391
BtuMI TCGCGA 1 cut(s) 1100
Cac8I GCNNGC 2 cut(s) 279, 1016
Cfr10I RCCGGY 1 cut(s) 1024
Cfr13I GGNCC 5 cut(s) 244, 266, 351, 1016, 1212
CseI GACGC 1 cut(s) 604
CsiI ACCWGGT 1 cut(s) 419
Csp6I GTAC 3 cut(s) 1059, 1147, 1443
CspAI ACCGGT 1 cut(s) 1024
CviAII CATG 7 cut(s) 53, 391, 769, 916, 1255, 1337, 1403
CviQI GTAC 3 cut(s) 1059, 1147, 1443
DdeI CTNAG 2 cut(s) 101, 113
DpnI GATC 7 cut(s) 330, 465, 830, 1206, 1317, 1371, 1466
DpnII GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 2 cut(s) 133, 902
Eam1104I CTCTTC 3 cut(s) 91, 165, 978
EarI CTCTTC 3 cut(s) 91, 165, 978
Eco130I CCWWGG 3 cut(s) 390, 958, 1150
Eco147I AGGCCT 1 cut(s) 475
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 698
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoO65I GGTNACC 1 cut(s) 698
EcoRII CCWGG 4 cut(s) 419, 694, 904, 927
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 390, 958, 1150
EcoT22I ATGCAT 2 cut(s) 530, 1309
ErhI CCWWGG 3 cut(s) 390, 958, 1150
FaeI CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
FaqI GGGAC 1 cut(s) 874
FatI CATG 7 cut(s) 52, 390, 768, 915, 1254, 1336, 1402
FauI CCCGC 1 cut(s) 1007
FbaI TGATCA 1 cut(s) 1464
Fnu4HI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
FokI GGATG 6 cut(s) 389, 726, 847, 949, 1001, 1081
Fsp4HI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
FspBI CTAG 6 cut(s) 893, 945, 959, 1319, 1416, 1424
GluI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
GsaI CCCAGC 1 cut(s) 1068
GsuI CTGGAG 1 cut(s) 185
HapII CCGG 1 cut(s) 1025
HgaI GACGC 1 cut(s) 604
Hin1I GRCGYC 1 cut(s) 615
Hin1II CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
HinfI GANTC 4 cut(s) 193, 782, 1105, 1341
HpaII CCGG 1 cut(s) 1025
Hpy166II GTNNAC 2 cut(s) 244, 1075
Hpy188I TCNGA 3 cut(s) 64, 301, 1008
Hpy8I GTNNAC 2 cut(s) 244, 1075
HpyAV CCTTC 4 cut(s) 181, 454, 1089, 1287
HpyCH4III ACNGT 5 cut(s) 365, 634, 1185, 1359, 1395
HpyCH4IV ACGT 2 cut(s) 570, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 612, 1412
HpyF3I CTNAG 2 cut(s) 101, 113
HpySE526I ACGT 2 cut(s) 570, 887
Hsp92I GRCGYC 1 cut(s) 615
Hsp92II CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
Ksp22I TGATCA 1 cut(s) 1464
Kzo9I GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 4 cut(s) 97, 166, 1035, 1123
Lsp1109I GCAGC 4 cut(s) 504, 511, 1124, 1127
LweI GCATC 3 cut(s) 634, 653, 987
MabI ACCWGGT 1 cut(s) 419
MaeI CTAG 6 cut(s) 893, 945, 959, 1319, 1416, 1424
MaeII ACGT 2 cut(s) 570, 887
MaeIII GTNAC 2 cut(s) 616, 698
MalI GATC 7 cut(s) 330, 465, 830, 1206, 1317, 1371, 1466
MboI GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
MflI RGATCY 3 cut(s) 463, 828, 1204
MhlI GDGCHC 3 cut(s) 160, 1032, 1077
MluCI AATT 5 cut(s) 76, 200, 358, 621, 1050
MlyI GAGTC 1 cut(s) 1099
MmeI TCCRAC 1 cut(s) 1027
Mph1103I ATGCAT 2 cut(s) 530, 1309
MroXI GAANNNNTTC 1 cut(s) 1168
MseI TTAA 4 cut(s) 141, 344, 489, 972
MslI CAYNNNNRTG 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 1064
MspI CCGG 1 cut(s) 1025
MspR9I CCNGG 4 cut(s) 421, 696, 906, 929
Mva1269I GAATGC 1 cut(s) 1288
MvaI CCWGG 4 cut(s) 421, 696, 906, 929
MvnI CGCG 1 cut(s) 1100
MwoI GCNNNNNNNGC 3 cut(s) 278, 612, 1412
NcoI CCATGG 1 cut(s) 390
NdeII GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
NlaIII CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
NlaIV GGNNCC 1 cut(s) 174
NmeAIII GCCGAG 1 cut(s) 880
NmuCI GTSAC 1 cut(s) 616
NruI TCGCGA 1 cut(s) 1100
NsiI ATGCAT 2 cut(s) 530, 1309
NspI RCATGY 1 cut(s) 1258
NspV TTCGAA 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 32
PceI AGGCCT 1 cut(s) 475
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 576
PctI GAATGC 1 cut(s) 1288
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 3 cut(s) 193, 782, 1341
PflMI CCANNNNNTGG 1 cut(s) 53
PfoI TCCNGGA 1 cut(s) 927
PinAI ACCGGT 1 cut(s) 1024
PkrI GCNGC 5 cut(s) 266, 501, 519, 1114, 1117
PleI GAGTC 1 cut(s) 1099
PpsI GAGTC 1 cut(s) 1099
Psp1406I AACGTT 1 cut(s) 570
Psp6I CCWGG 4 cut(s) 419, 694, 904, 927
PspEI GGTNACC 1 cut(s) 698
PspFI CCCAGC 1 cut(s) 1064
PspGI CCWGG 4 cut(s) 419, 694, 904, 927
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 5 cut(s) 244, 266, 351, 1016, 1212
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 2 cut(s) 274, 522
PsuI RGATCY 3 cut(s) 463, 828, 1204
PvuII CAGCTG 1 cut(s) 1064
RruI TCGCGA 1 cut(s) 1100
RsaI GTAC 3 cut(s) 1060, 1148, 1444
RsaNI GTAC 3 cut(s) 1059, 1147, 1443
RseI CAYNNNNRTG 1 cut(s) 1007
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 4 cut(s) 141, 344, 489, 972
SatI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
Sau3AI GATC 7 cut(s) 328, 463, 828, 1204, 1315, 1369, 1464
Sau96I GGNCC 5 cut(s) 244, 266, 351, 1016, 1212
SbfI CCTGCAGG 1 cut(s) 274
SchI GAGTC 1 cut(s) 1099
ScrFI CCNGG 4 cut(s) 421, 696, 906, 929
SdaI CCTGCAGG 1 cut(s) 274
SduI GDGCHC 3 cut(s) 160, 1032, 1077
SexAI ACCWGGT 1 cut(s) 419
SfaNI GCATC 3 cut(s) 634, 653, 987
SfcI CTRYAG 3 cut(s) 124, 270, 518
Sfr274I CTCGAG 1 cut(s) 32
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 1 cut(s) 244
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 1007
SmlI CTYRAG 3 cut(s) 32, 503, 1268
SmoI CTYRAG 3 cut(s) 32, 503, 1268
Sse8387I CCTGCAGG 1 cut(s) 274
Sse9I AATT 5 cut(s) 76, 200, 358, 621, 1050
SseBI AGGCCT 1 cut(s) 475
SsiI CCGC 2 cut(s) 264, 1014
SspMI CTAG 6 cut(s) 893, 945, 959, 1319, 1416, 1424
StuI AGGCCT 1 cut(s) 475
StyD4I CCNGG 4 cut(s) 419, 694, 904, 927
StyI CCWWGG 3 cut(s) 390, 958, 1150
TaaI ACNGT 5 cut(s) 365, 634, 1185, 1359, 1395
TaiI ACGT 2 cut(s) 573, 890
TaqI TCGA 7 cut(s) 33, 198, 323, 411, 455, 981, 1354
TasI AATT 5 cut(s) 76, 200, 358, 621, 1050
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 3 cut(s) 193, 782, 1341
Tru1I TTAA 4 cut(s) 141, 344, 489, 972
Tru9I TTAA 4 cut(s) 141, 344, 489, 972
TscAI CASTG 2 cut(s) 613, 1398
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 4 cut(s) 499, 517, 1112, 1115
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 8 cut(s) 41, 430, 462, 539, 757, 849, 932, 1194
TspRI CASTG 2 cut(s) 613, 1398
Van91I CCANNNNNTGG 1 cut(s) 53
VneI GTGCAC 1 cut(s) 1073
VpaK11BI GGWCC 1 cut(s) 244
XagI CCTNNNNNAGG 1 cut(s) 1083
XapI RAATTY 1 cut(s) 200
XbaI TCTAGA 1 cut(s) 1318
XceI RCATGY 1 cut(s) 1258
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 958
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 6 cut(s) 893, 945, 959, 1319, 1416, 1424
Zsp2I ATGCAT 2 cut(s) 530, 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.