Rh2AG313400

Lycopene beta cyclase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007985
Physical Location & Seq
Forward (+)
40216749 .. 40218940
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG313400.1

Sequence Viewer

Length: 1494 bp
ATGGATACATTACTCAAAACACATAACAAGCTCGAGTTTCTGCACCCACTTCATGGGTTTTCAGAGAAAGTGAGTAATTGGAACTCTTCGAGGCAGGAGCTTCGATGTGGTCTCAGGAAGTCGTATAGAACTGGTCGTGTTAAGGCTAGTAGTAGTGCGCTTCTGGAGCTTGTGCCTGAAACCAAGAAGGAGACTCTCGAGTTTGAGCTTCCCTTGTATGACCCATCAAAGGGTCTTGTGGTGGACCTTGCAGTCGTGGGAGGCGGCCCTGCAGGGCTTGCAGTGGCACAGCAAGTCTCGGAGGCAGGGCTCTCCGTTTGCTCGATTGATCCCTCTCCCAAGTTAATCTGGCCCAACAATTATGGTGTTTGGGTGGATGAGTTTGAGGCCATGGATCTGCTTGATTGCCTCGACACTACCTGGTCTGGTGCTGTTGTGTTTATAGATGAGCAATCGAAGAAGGATCTTGACAGGCCTTATGGGAGAGTTAATAGGAAGCAGCTCAAGTCCAAAATGCTGCAGAAATGCATTTCAAATGGTGTCAAGTTTCATCAAGCTAAAGTCAGTAACGTTATTCACGAGGAGGAAAAATCACTCTTGATTTGCAACGATGGCATCACAATTCAAGCTTCTGTGGTTCTTGATGCAACAGGTTTTTCAAGATGCCTTGTACAGTATGATAAGCCTTACAATCCAGGTTACCAAGTGGCGTATGGGATTTTGGCAGAGGTCGAAGAGCATCCATTTGATGTGGATAAGATGGTTTTCATGGATTGGAGAGATTCGCATTTGAAGAATAGTCCTGAACTGAAAGAGAGAAATAGTAAGATCCCTACTTTTCTATATGCAATGCCTTTTTCATCCAACAGGATATTTCTTGAAGAAACCTCTCTAGTTGCTCGGCCTGGACTACCCATGAAAGATATCCAGGAAAGGATGGTTGCTAGATTGAGGCACCTAGGCATAAAAGTTAAGAGCATTGAAGAGGATGAGCATTGTGTCATTCCAATGGGCGGGCCCCTCCCGGTGCTCCCTCAAAGAGTTGTTGGAATTGGCGGTACAGCTGGGATGGTGCACCCTTCAACAGGGTATATGGTTGCGAGGACTCTGGCAGCAGCTCCTATTGTTGCAAATGCCATAGTTCAGTACCTTGGTTCTGATAAAAGTCTTTCAGGAAATGAACTTTCAGCAGAAGTTTGGAAAGATTTATGGCCCATACAAAGGAGGAGACAAAGGGAGTTCTTCTGTTTCGGCATGTCCATTCTGCTCAAGCTGGATTTGAATGCCACAAGAAGGTTTTTCAATGCATTTTTTGATCTAGAACCTCGTTATTGGCATGGATTCTTGTCATCTCGACTGTTTCTTCCCGATCTTGTATTTTTCGGGCTTTCACTGTTTTCTCATGCTTCAAATGCTAGTAGATTAGAAATAATGGGAAAGGGAACTATTCCCTTGGTAAAGATGATCAACAACTTGATACAGGATAGAGATTAG

Protein Analysis

497

Amino Acids

55.91

Weight (kDa)

7.62

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lycopene_cycl PF05834 82 - 474 6.4e-149 Lycopene cyclase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 954
AccB7I CCANNNNNTGG 1 cut(s) 53
AciI CCGC 3 cut(s) 264, 1014, 1056
AclI AACGTT 1 cut(s) 570
AclWI GGATC 4 cut(s) 323, 402, 471, 823
AfaI GTAC 3 cut(s) 672, 1060, 1148
AfiI CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
AjnI CCWGG 4 cut(s) 419, 694, 904, 927
Alw21I GWGCWC 2 cut(s) 1032, 1077
Alw26I GTCTC 4 cut(s) 116, 185, 301, 1222
Alw44I GTGCAC 1 cut(s) 1073
AlwI GGATC 4 cut(s) 323, 402, 471, 823
Ama87I CYCGRG 2 cut(s) 32, 197
AoxI GGCC 7 cut(s) 265, 350, 387, 473, 902, 1016, 1211
ApaI GGGCCC 1 cut(s) 1020
ApaLI GTGCAC 1 cut(s) 1073
ApeKI GCWGC 4 cut(s) 499, 517, 1112, 1115
AspA2I CCTAGG 1 cut(s) 958
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
AsuC2I CCSGG 1 cut(s) 1025
AvaI CYCGRG 2 cut(s) 32, 197
AvaII GGWCC 1 cut(s) 244
AvrII CCTAGG 1 cut(s) 958
BaeGI GKGCMC 2 cut(s) 1020, 1077
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 2 cut(s) 312, 1020
BauI CACGAG 1 cut(s) 578
Bbv12I GWGCWC 2 cut(s) 1032, 1077
BbvI GCAGC 4 cut(s) 504, 511, 1124, 1127
BccI CCATC 5 cut(s) 232, 605, 754, 931, 1063
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 4 cut(s) 421, 696, 906, 929
BclI TGATCA 1 cut(s) 1464
BcnI CCSGG 1 cut(s) 1025
BcoDI GTCTC 4 cut(s) 116, 185, 301, 1222
BfaI CTAG 6 cut(s) 147, 893, 945, 959, 1319, 1416
BfmI CTRYAG 2 cut(s) 270, 518
BisI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
BlnI CCTAGG 1 cut(s) 958
BlsI GCNGC 5 cut(s) 266, 501, 519, 1114, 1117
Bme1390I CCNGG 5 cut(s) 421, 696, 906, 929, 1025
Bme18I GGWCC 1 cut(s) 244
BmeT110I CYCGRG 2 cut(s) 32, 197
BmgT120I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
BmiI GGNNCC 3 cut(s) 956, 1018, 1019
BmrFI CCNGG 5 cut(s) 421, 696, 906, 929, 1025
BmsI GCATC 4 cut(s) 624, 634, 653, 748
BpmI CTGGAG 1 cut(s) 185
BpuEI CTTGAG 2 cut(s) 488, 1253
BpuMI CCSGG 1 cut(s) 1025
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 4 cut(s) 390, 958, 1150, 1452
Bsc4I CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 4 cut(s) 421, 696, 906, 929
BseDI CCNNGG 4 cut(s) 390, 958, 1150, 1452
BseGI GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BseLI CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 596, 1240
BseSI GKGCMC 2 cut(s) 1020, 1077
BseXI GCAGC 4 cut(s) 504, 511, 1124, 1127
BseYI CCCAGC 1 cut(s) 1064
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
BshNI GGYRCC 1 cut(s) 954
BsiHKAI GWGCWC 2 cut(s) 1032, 1077
BsiHKCI CYCGRG 2 cut(s) 32, 197
BsiSI CCGG 1 cut(s) 1025
BslI CCNNNNNNNGG 7 cut(s) 53, 229, 230, 1013, 1085, 1221, 1293
BsmAI GTCTC 4 cut(s) 116, 185, 301, 1222
BsmI GAATGC 1 cut(s) 1288
BsnI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
Bso31I GGTCTC 1 cut(s) 116
BsoBI CYCGRG 2 cut(s) 32, 197
Bsp120I GGGCCC 1 cut(s) 1016
Bsp1286I GDGCHC 4 cut(s) 312, 1020, 1032, 1077
Bsp1407I TGTACA 1 cut(s) 670
Bsp143I GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 3 cut(s) 264, 1014, 1056
BspANI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
BspCNI CTCAG 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 956, 1018, 1019
BspMAI CTGCAG 2 cut(s) 274, 522
BspPI GGATC 4 cut(s) 323, 402, 471, 823
BspQI GCTCTTC 1 cut(s) 729
BspT107I GGYRCC 1 cut(s) 954
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 855
BsrGI TGTACA 1 cut(s) 670
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 4 cut(s) 390, 958, 1150, 1452
BssMI GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 4 cut(s) 390, 958, 1150, 1452
Bst2BI CACGAG 1 cut(s) 578
Bst2UI CCWGG 4 cut(s) 421, 696, 906, 929
Bst4CI ACNGT 3 cut(s) 675, 1359, 1395
Bst6I CTCTTC 3 cut(s) 91, 729, 978
BstAPI GCANNNNNTGC 1 cut(s) 278
BstAUI TGTACA 1 cut(s) 670
BstC8I GCNNGC 2 cut(s) 279, 1016
BstDEI CTNAG 1 cut(s) 113
BstDSI CCRYGG 1 cut(s) 390
BstEII GGTNACC 1 cut(s) 698
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 7 cut(s) 331, 397, 466, 831, 1318, 1372, 1467
BstMAI GTCTC 4 cut(s) 116, 185, 301, 1222
BstMBI GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
BstMWI GCNNNNNNNGC 4 cut(s) 166, 278, 612, 1412
BstNI CCWGG 4 cut(s) 421, 696, 906, 929
BstNSI RCATGY 1 cut(s) 1258
BstPI GGTNACC 1 cut(s) 698
BstSCI CCNGG 5 cut(s) 419, 694, 904, 927, 1023
BstSFI CTRYAG 2 cut(s) 270, 518
BstSLI GKGCMC 2 cut(s) 1020, 1077
BstV1I GCAGC 4 cut(s) 504, 511, 1124, 1127
BstX2I RGATCY 3 cut(s) 394, 463, 828
BstYI RGATCY 3 cut(s) 394, 463, 828
BsuRI GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 6 cut(s) 382, 739, 860, 942, 994, 1074
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 288, 1391
Cac8I GCNNGC 2 cut(s) 279, 1016
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
CsiI ACCWGGT 1 cut(s) 419
Csp6I GTAC 3 cut(s) 671, 1059, 1147
CviAII CATG 7 cut(s) 53, 391, 769, 916, 1255, 1337, 1403
CviQI GTAC 3 cut(s) 671, 1059, 1147
DdeI CTNAG 1 cut(s) 113
DpnI GATC 7 cut(s) 330, 396, 465, 830, 1317, 1371, 1466
DpnII GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
Eam1104I CTCTTC 3 cut(s) 91, 729, 978
EarI CTCTTC 3 cut(s) 91, 729, 978
Eco130I CCWWGG 4 cut(s) 390, 958, 1150, 1452
Eco147I AGGCCT 1 cut(s) 475
Eco24I GRGCYC 2 cut(s) 312, 1020
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 1 cut(s) 925
Eco47I GGWCC 1 cut(s) 244
Eco88I CYCGRG 2 cut(s) 32, 197
Eco91I GGTNACC 1 cut(s) 698
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoO109I RGGNCCY 1 cut(s) 1017
EcoO65I GGTNACC 1 cut(s) 698
EcoRII CCWGG 4 cut(s) 419, 694, 904, 927
EcoRV GATATC 1 cut(s) 925
EcoT14I CCWWGG 4 cut(s) 390, 958, 1150, 1452
EcoT22I ATGCAT 2 cut(s) 530, 1309
EcoT38I GRGCYC 2 cut(s) 312, 1020
ErhI CCWWGG 4 cut(s) 390, 958, 1150, 1452
FaeI CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
FatI CATG 7 cut(s) 52, 390, 768, 915, 1254, 1336, 1402
FauI CCCGC 1 cut(s) 1007
FbaI TGATCA 1 cut(s) 1464
Fnu4HI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
FokI GGATG 6 cut(s) 389, 726, 847, 949, 1001, 1081
FriOI GRGCYC 2 cut(s) 312, 1020
Fsp4HI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
FspBI CTAG 6 cut(s) 147, 893, 945, 959, 1319, 1416
GlaI GCGC 1 cut(s) 159
GluI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
GsaI CCCAGC 1 cut(s) 1068
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 7 cut(s) 267, 352, 389, 475, 904, 1018, 1213
HapII CCGG 1 cut(s) 1025
HhaI GCGC 1 cut(s) 160
Hin1II CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HindIII AAGCTT 1 cut(s) 627
HinfI GANTC 4 cut(s) 193, 782, 1105, 1341
HpaII CCGG 1 cut(s) 1025
Hpy166II GTNNAC 2 cut(s) 244, 1075
Hpy188I TCNGA 3 cut(s) 64, 301, 1159
Hpy8I GTNNAC 2 cut(s) 244, 1075
HpyAV CCTTC 4 cut(s) 181, 454, 1089, 1287
HpyCH4III ACNGT 3 cut(s) 675, 1359, 1395
HpyCH4IV ACGT 1 cut(s) 570
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 278, 612, 1412
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
HspAI GCGC 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 1464
Kzo9I GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 4 cut(s) 97, 166, 1035, 1123
Lsp1109I GCAGC 4 cut(s) 504, 511, 1124, 1127
LweI GCATC 4 cut(s) 624, 634, 653, 748
MabI ACCWGGT 1 cut(s) 419
MaeI CTAG 6 cut(s) 147, 893, 945, 959, 1319, 1416
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 2 cut(s) 566, 698
MalI GATC 7 cut(s) 330, 396, 465, 830, 1317, 1371, 1466
MboI GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
MboII GAAGA 8 cut(s) 78, 469, 746, 805, 893, 995, 1234, 1355
MflI RGATCY 3 cut(s) 394, 463, 828
MhlI GDGCHC 4 cut(s) 312, 1020, 1032, 1077
MluCI AATT 4 cut(s) 76, 358, 621, 1050
MlyI GAGTC 2 cut(s) 187, 1099
MmeI TCCRAC 2 cut(s) 888, 1027
Mph1103I ATGCAT 2 cut(s) 530, 1309
MseI TTAA 4 cut(s) 141, 344, 489, 972
MslI CAYNNNNRTG 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 1064
MspI CCGG 1 cut(s) 1025
MspR9I CCNGG 5 cut(s) 421, 696, 906, 929, 1025
Mva1269I GAATGC 1 cut(s) 1288
MvaI CCWGG 4 cut(s) 421, 696, 906, 929
MwoI GCNNNNNNNGC 4 cut(s) 166, 278, 612, 1412
NciI CCSGG 1 cut(s) 1025
NcoI CCATGG 1 cut(s) 390
NdeII GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
NlaIII CATG 7 cut(s) 56, 394, 772, 919, 1258, 1340, 1406
NlaIV GGNNCC 3 cut(s) 956, 1018, 1019
NmeAIII GCCGAG 1 cut(s) 880
NsiI ATGCAT 2 cut(s) 530, 1309
NspI RCATGY 1 cut(s) 1258
PaeR7I CTCGAG 2 cut(s) 32, 197
PceI AGGCCT 1 cut(s) 475
PciSI GCTCTTC 1 cut(s) 729
PcsI WCGNNNNNNNCGW 1 cut(s) 576
PctI GAATGC 1 cut(s) 1288
PfeI GAWTC 2 cut(s) 782, 1341
PflMI CCANNNNNTGG 1 cut(s) 53
PfoI TCCNGGA 1 cut(s) 927
PkrI GCNGC 5 cut(s) 266, 501, 519, 1114, 1117
PleI GAGTC 2 cut(s) 187, 1099
PpsI GAGTC 2 cut(s) 187, 1099
Psp1406I AACGTT 1 cut(s) 570
Psp6I CCWGG 4 cut(s) 419, 694, 904, 927
PspEI GGTNACC 1 cut(s) 698
PspFI CCCAGC 1 cut(s) 1064
PspGI CCWGG 4 cut(s) 419, 694, 904, 927
PspN4I GGNNCC 3 cut(s) 956, 1018, 1019
PspOMI GGGCCC 1 cut(s) 1016
PspPI GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 2 cut(s) 274, 522
PsuI RGATCY 3 cut(s) 394, 463, 828
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 3 cut(s) 672, 1060, 1148
RsaNI GTAC 3 cut(s) 671, 1059, 1147
RseI CAYNNNNRTG 1 cut(s) 1007
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 4 cut(s) 141, 344, 489, 972
SatI GCNGC 5 cut(s) 265, 500, 518, 1113, 1116
Sau3AI GATC 7 cut(s) 328, 394, 463, 828, 1315, 1369, 1464
Sau96I GGNCC 6 cut(s) 244, 266, 351, 1016, 1017, 1212
SbfI CCTGCAGG 1 cut(s) 274
SchI GAGTC 2 cut(s) 187, 1099
ScrFI CCNGG 5 cut(s) 421, 696, 906, 929, 1025
SdaI CCTGCAGG 1 cut(s) 274
SduI GDGCHC 4 cut(s) 312, 1020, 1032, 1077
SexAI ACCWGGT 1 cut(s) 419
SfaNI GCATC 4 cut(s) 624, 634, 653, 748
SfcI CTRYAG 2 cut(s) 270, 518
Sfr274I CTCGAG 2 cut(s) 32, 197
SinI GGWCC 1 cut(s) 244
SlaI CTCGAG 2 cut(s) 32, 197
SmiMI CAYNNNNRTG 1 cut(s) 1007
SmlI CTYRAG 4 cut(s) 32, 197, 503, 1268
SmoI CTYRAG 4 cut(s) 32, 197, 503, 1268
Sse8387I CCTGCAGG 1 cut(s) 274
Sse9I AATT 4 cut(s) 76, 358, 621, 1050
SseBI AGGCCT 1 cut(s) 475
SsiI CCGC 3 cut(s) 264, 1014, 1056
SspMI CTAG 6 cut(s) 147, 893, 945, 959, 1319, 1416
StuI AGGCCT 1 cut(s) 475
StyD4I CCNGG 5 cut(s) 419, 694, 904, 927, 1023
StyI CCWWGG 4 cut(s) 390, 958, 1150, 1452
TaaI ACNGT 3 cut(s) 675, 1359, 1395
TaiI ACGT 1 cut(s) 573
TaqI TCGA 9 cut(s) 33, 89, 103, 198, 323, 411, 455, 732, 1354
TasI AATT 4 cut(s) 76, 358, 621, 1050
TatI WGTACW 1 cut(s) 670
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 2 cut(s) 782, 1341
Tru1I TTAA 4 cut(s) 141, 344, 489, 972
Tru9I TTAA 4 cut(s) 141, 344, 489, 972
TscAI CASTG 2 cut(s) 288, 1398
TseI GCWGC 4 cut(s) 499, 517, 1112, 1115
TspDTI ATGAA 6 cut(s) 41, 539, 757, 849, 932, 1194
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 2 cut(s) 288, 1398
Van91I CCANNNNNTGG 1 cut(s) 53
VneI GTGCAC 1 cut(s) 1073
VpaK11BI GGWCC 1 cut(s) 244
XagI CCTNNNNNAGG 1 cut(s) 1083
XbaI TCTAGA 1 cut(s) 1318
XceI RCATGY 1 cut(s) 1258
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XhoI CTCGAG 2 cut(s) 32, 197
XmaJI CCTAGG 1 cut(s) 958
XspI CTAG 6 cut(s) 147, 893, 945, 959, 1319, 1416
Zsp2I ATGCAT 2 cut(s) 530, 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.