FvH4_6g27740
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014034
Physical Location & Seq
Forward (+)
21379620 .. 21385562
5943 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27740.t2

Sequence Viewer

Length: 807 bp
ATGGAGGAGTACAACCCTGGATTTTACAGAAACCCAGTTTTCAGTGATCCACAGGAAGCAGAAACCGTCTCAGAAAATGATCCGGACTCGCCCTCCTCCGGGGAGGAGACAAAGGTGTTCACTACTGCACCATCACCCAAGAAAAGGAGGGGAGTAGTGCAGAAGAGGGTCGTGTCAGTTCCAATCAGAGAGATGGAAGGATCCAAGGGAAAAGGAGATGGAAATCCACCTTCTGATTCATGGACTTGGAGGAAGTACGGCCAGAAACCTATCAAAGGGTCTCCATATCCAAGGGGATACTATCGATGTAGTTCTTCCAAGGCTTGTCCGGCGAGGAAACAAGTGGAGAGAAGCCGTTCGGACCCAAGAATGCTCTTGATCACCTACGCTTGCGAGCACAACCACCACATGCCAACCACCAAAGGATCCCACTCCGCCGCCTTAACCGACCCTGTAAATGCGACATCACCCACCAAAGAGTTTGAACTTCCCGATAGAGTTGCACCTATAGAGGAGCTCACCCCCATTGCAAGTCAAGTCGACCTCGAGCTTAGTGGTGACTCGGGGGCGTTGCTTGGCGACAATGGTTGGTTCGACGACATGGAGACGACAATTGTACTAGAAAGTTCATTTTGTGTAGGAAATAGCGACCTTGTGGACATGGATGTGGCAATGAGGTTGGAACATGAAGACGAGTATTTATTTGCTGATTTAGGCGAGTTACCTGAGAGTTCAGTCATTTTTAGACATAGGAAGCTAAACTCAGACGAACAAAATCAACGGCGTAGTGTAAGTATTACTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

269

Amino Acids

29.9

Weight (kDa)

5.12

Isoelectric Point (pI)

53.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 79 - 131 1.1e-18 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 540
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 2 cut(s) 435, 438
AclWI GGATC 6 cut(s) 41, 74, 195, 208, 420, 433
AcoI YGGCCR 1 cut(s) 259
AfaI GTAC 3 cut(s) 11, 257, 618
AfiI CCNNNNNNNGG 4 cut(s) 98, 99, 144, 275
AgsI TTSAA 1 cut(s) 485
AjnI CCWGG 1 cut(s) 16
AluBI AGCT 3 cut(s) 517, 550, 757
AluI AGCT 3 cut(s) 517, 550, 757
Alw21I GWGCWC 2 cut(s) 399, 519
Alw26I GTCTC 4 cut(s) 73, 101, 285, 599
AlwI GGATC 6 cut(s) 41, 74, 195, 208, 420, 433
Ama87I CYCGRG 2 cut(s) 545, 562
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 1 cut(s) 259
Asp700I GAANNNNTTC 1 cut(s) 355
AspS9I GGNCC 1 cut(s) 361
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 5 cut(s) 126, 373, 459, 511, 569
AvaI CYCGRG 2 cut(s) 545, 562
AvaII GGWCC 1 cut(s) 361
BamHI GGATCC 2 cut(s) 200, 425
BanII GRGCYC 1 cut(s) 519
BbsI GAAGAC 1 cut(s) 696
Bbv12I GWGCWC 2 cut(s) 399, 519
BccI CCATC 3 cut(s) 139, 187, 212
BceAI ACGGC 3 cut(s) 274, 339, 797
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 1 cut(s) 18
BciVI GTATCC 1 cut(s) 290
BclI TGATCA 1 cut(s) 378
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 4 cut(s) 73, 101, 285, 599
BfaI CTAG 1 cut(s) 620
BfmI CTRYAG 1 cut(s) 507
BfuI GTATCC 1 cut(s) 290
BisI GCNGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 439
Bme1390I CCNGG 2 cut(s) 18, 100
Bme18I GGWCC 1 cut(s) 361
BmeT110I CYCGRG 2 cut(s) 545, 562
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 3 cut(s) 202, 363, 427
BmrFI CCNGG 2 cut(s) 18, 100
BmrI ACTGGG 1 cut(s) 29
BmuI ACTGGG 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 696
BpuMI CCSGG 1 cut(s) 100
Bsa29I ATCGAT 1 cut(s) 304
BsaBI GATNNNNATC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 285
BsaJI CCNNGG 5 cut(s) 16, 99, 204, 290, 318
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 99, 144, 275
Bse1I ACTGG 1 cut(s) 35
Bse3DI GCAATG 2 cut(s) 525, 678
Bse8I GATNNNNATC 1 cut(s) 222
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 1 cut(s) 18
BseCI ATCGAT 1 cut(s) 304
BseDI CCNNGG 5 cut(s) 16, 99, 204, 290, 318
BseGI GGATG 1 cut(s) 670
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 4 cut(s) 98, 99, 144, 275
BseMI GCAATG 2 cut(s) 525, 678
BseMII CTCAG 3 cut(s) 84, 717, 777
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 4 cut(s) 20, 85, 119, 527
BsgI GTGCAG 2 cut(s) 111, 179
BshFI GGCC 1 cut(s) 261
BshVI ATCGAT 1 cut(s) 304
BsiHKAI GWGCWC 2 cut(s) 399, 519
BsiHKCI CYCGRG 2 cut(s) 545, 562
BsiSI CCGG 3 cut(s) 83, 99, 329
BslI CCNNNNNNNGG 4 cut(s) 98, 99, 144, 275
BsmAI GTCTC 4 cut(s) 73, 101, 285, 599
BsmBI CGTCTC 2 cut(s) 73, 599
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 1 cut(s) 261
Bso31I GGTCTC 1 cut(s) 285
BsoBI CYCGRG 2 cut(s) 545, 562
Bsp1286I GDGCHC 2 cut(s) 399, 519
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 5 cut(s) 46, 79, 200, 378, 425
BspACI CCGC 2 cut(s) 435, 438
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 3 cut(s) 83, 718, 776
BspDI ATCGAT 1 cut(s) 304
BspEI TCCGGA 1 cut(s) 82
BspLI GGNNCC 3 cut(s) 202, 363, 427
BspPI GGATC 6 cut(s) 41, 74, 195, 208, 420, 433
BspTNI GGTCTC 1 cut(s) 285
BsrDI GCAATG 2 cut(s) 525, 678
BsrI ACTGG 1 cut(s) 35
BssECI CCNNGG 5 cut(s) 16, 99, 204, 290, 318
BssMI GATC 5 cut(s) 46, 79, 200, 378, 425
BssT1I CCWWGG 3 cut(s) 204, 290, 318
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 391, 395
BstDEI CTNAG 4 cut(s) 70, 551, 726, 763
BstENI CCTNNNNNAGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 670
BstKTI GATC 5 cut(s) 49, 82, 203, 381, 428
BstMAI GTCTC 4 cut(s) 73, 101, 285, 599
BstMBI GATC 5 cut(s) 46, 79, 200, 378, 425
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 18
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 2 cut(s) 16, 98
BstSFI CTRYAG 1 cut(s) 507
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 2 cut(s) 200, 425
BstYI RGATCY 2 cut(s) 200, 425
Bsu15I ATCGAT 1 cut(s) 304
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 1 cut(s) 261
BsuTUI ATCGAT 1 cut(s) 304
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 2 cut(s) 391, 395
Cfr13I GGNCC 1 cut(s) 361
ClaI ATCGAT 1 cut(s) 304
Csp6I GTAC 3 cut(s) 10, 256, 617
CviAII CATG 5 cut(s) 240, 409, 601, 661, 686
CviJI RGCY 6 cut(s) 261, 323, 354, 517, 550, 757
CviKI_1 RGCY 6 cut(s) 261, 323, 354, 517, 550, 757
CviQI GTAC 3 cut(s) 10, 256, 617
DdeI CTNAG 4 cut(s) 70, 551, 726, 763
DpnI GATC 5 cut(s) 48, 81, 202, 380, 427
DpnII GATC 5 cut(s) 46, 79, 200, 378, 425
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 424
Ecl136II GAGCTC 1 cut(s) 517
Eco130I CCWWGG 3 cut(s) 204, 290, 318
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 517
Eco88I CYCGRG 2 cut(s) 545, 562
EcoICRI GAGCTC 1 cut(s) 517
EcoNI CCTNNNNNAGG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 16
EcoT14I CCWWGG 3 cut(s) 204, 290, 318
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 3 cut(s) 204, 290, 318
Esp3I CGTCTC 2 cut(s) 73, 599
FaeI CATG 5 cut(s) 243, 412, 604, 664, 689
FaiI YATR 9 cut(s) 241, 286, 410, 509, 602, 662, 687, 750, 805
FatI CATG 5 cut(s) 239, 408, 600, 660, 685
FbaI TGATCA 1 cut(s) 378
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 1 cut(s) 438
FokI GGATG 1 cut(s) 677
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 1 cut(s) 438
FspBI CTAG 1 cut(s) 620
GluI GCNGC 1 cut(s) 438
HaeIII GGCC 1 cut(s) 261
HapII CCGG 3 cut(s) 83, 99, 329
Hin1II CATG 5 cut(s) 243, 412, 604, 664, 689
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 3 cut(s) 86, 236, 560
HpaII CCGG 3 cut(s) 83, 99, 329
HphI GGTGA 5 cut(s) 126, 373, 459, 511, 569
Hpy166II GTNNAC 3 cut(s) 120, 541, 658
Hpy188I TCNGA 5 cut(s) 73, 188, 235, 361, 766
Hpy188III TCNNGA 3 cut(s) 83, 376, 491
Hpy8I GTNNAC 3 cut(s) 120, 541, 658
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 2 cut(s) 191, 240
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4V TGCA 4 cut(s) 128, 160, 503, 530
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 70, 551, 726, 763
Hsp92II CATG 5 cut(s) 243, 412, 604, 664, 689
Kpn2I TCCGGA 1 cut(s) 82
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 5 cut(s) 46, 79, 200, 378, 425
LmnI GCTCC 1 cut(s) 514
MaeI CTAG 1 cut(s) 620
MaeIII GTNAC 2 cut(s) 557, 720
MalI GATC 5 cut(s) 48, 81, 202, 380, 427
MboI GATC 5 cut(s) 46, 79, 200, 378, 425
MboII GAAGA 3 cut(s) 175, 306, 701
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 2 cut(s) 200, 425
MhlI GDGCHC 2 cut(s) 399, 519
MluCI AATT 1 cut(s) 612
MlyI GAGTC 2 cut(s) 80, 554
MmeI TCCRAC 1 cut(s) 660
MroI TCCGGA 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 355
MseI TTAA 1 cut(s) 443
MslI CAYNNNNRTG 1 cut(s) 665
MspI CCGG 3 cut(s) 83, 99, 329
MspR9I CCNGG 2 cut(s) 18, 100
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 1 cut(s) 18
MwoI GCNNNNNNNGC 1 cut(s) 329
NciI CCSGG 1 cut(s) 100
NdeII GATC 5 cut(s) 46, 79, 200, 378, 425
NlaIII CATG 5 cut(s) 243, 412, 604, 664, 689
NlaIV GGNNCC 3 cut(s) 202, 363, 427
NmuCI GTSAC 1 cut(s) 557
NspI RCATGY 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 545
PctI GAATGC 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 1 cut(s) 439
PleI GAGTC 2 cut(s) 80, 554
PpsI GAGTC 2 cut(s) 80, 554
Psp124BI GAGCTC 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 3 cut(s) 202, 363, 427
PspPI GGNCC 1 cut(s) 361
PspXI VCTCGAGB 1 cut(s) 545
PsuI RGATCY 2 cut(s) 200, 425
RsaI GTAC 3 cut(s) 11, 257, 618
RsaNI GTAC 3 cut(s) 10, 256, 617
RseI CAYNNNNRTG 1 cut(s) 665
SacI GAGCTC 1 cut(s) 519
SalI GTCGAC 1 cut(s) 539
SaqAI TTAA 1 cut(s) 443
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 5 cut(s) 46, 79, 200, 378, 425
Sau96I GGNCC 1 cut(s) 361
SchI GAGTC 2 cut(s) 80, 554
ScrFI CCNGG 2 cut(s) 18, 100
SduI GDGCHC 2 cut(s) 399, 519
SfcI CTRYAG 1 cut(s) 507
Sfr274I CTCGAG 1 cut(s) 545
SinI GGWCC 1 cut(s) 361
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 665
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 1 cut(s) 612
SsiI CCGC 2 cut(s) 435, 438
SspMI CTAG 1 cut(s) 620
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 2 cut(s) 16, 98
StyI CCWWGG 3 cut(s) 204, 290, 318
TaaI ACNGT 1 cut(s) 67
TaqI TCGA 4 cut(s) 304, 540, 546, 594
TasI AATT 1 cut(s) 612
TatI WGTACW 2 cut(s) 9, 616
TauI GCSGC 1 cut(s) 440
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 443
Tru9I TTAA 1 cut(s) 443
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 1 cut(s) 557
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 3 cut(s) 228, 618, 702
TspRI CASTG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 361
XagI CCTNNNNNAGG 1 cut(s) 273
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 355
XspI CTAG 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.