Prupe.3G113300_v2.0.a1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0014034
Physical Location & Seq
Reverse (-)
9469832 .. 9473198
3367 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G113300.1

Sequence Viewer

Length: 807 bp
ATGGACCCTAAATTCTACAGAATTTCCCCATTTGGGACTGACCCAGCAGACCCTGATCAGCCCATGACGTCAGAGAATGGTCCGGGCTCTCCCTCCTCAGGGGAGGAGACAAAGGTGGCCACTGCCCCATCACCCAAGAAAAGGAGGGGAGTGCAGAAGAGGGTGGTGACAGTACCGATCGGTGACGTGGAGGGATCCAAGAGCAAAGGGGAGGGCCATCCACCGTCCGATTCTTGGGCTTGGAGGAAGTACGGCCAAAAGCCCATTAAAGGGTCTCCATATCCCAGGGGATATTATCGATGTAGTAGCTCCAAGGGTTGCCCGGCAAGGAAACAAGTGGAGAGAAGTCGTGTGGACCCCACAATGCTCTTGATCACCTATGCTTGTGAGCACAACCACCCCAAGCCCACCACCAAGCCACACCAAACCTCCACCGCCATGTCACCCAACGCCGAACCCGAACTGCCCGCCAAAACAATCACACCCAATGATGAGGAGCTCACAATTTTTGCGAGCCAAGTCGACCTTGACCTTAGTGACGACTCGGCCACGTTGCTTAGCGCCTTCGGTTGGTTCAGTGACGTGGCATCGACAGCTGTTCTAGAAAGTCCGATTTGTGCGGGAAATAGTAGTTGCGCTGATTATGACGTGGCAACGAGGTTGGGAGATGAGGAAGATGAGTATTTGTTCGCCGACCTGGGCGAGTTACCAGAGGGTTCAGTGATTTTCCGACACAAGATGGTGGAATCGGACGAGCAGAAACGGAGATGTAGTTTAAGTGTAGTTCCTTGTTGCGATAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

269

Amino Acids

29.25

Weight (kDa)

5.36

Isoelectric Point (pI)

58.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 522
AciI CCGC 3 cut(s) 435, 468, 620
AclWI GGATC 2 cut(s) 189, 202
AcoI YGGCCR 3 cut(s) 117, 253, 546
AcsI RAATTY 2 cut(s) 11, 21
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 2 cut(s) 174, 251
AfiI CCNNNNNNNGG 8 cut(s) 33, 98, 99, 141, 234, 269, 270, 570
AjiI CACGTC 3 cut(s) 187, 583, 649
AjnI CCWGG 2 cut(s) 284, 696
AluBI AGCT 3 cut(s) 309, 499, 596
AluI AGCT 3 cut(s) 309, 499, 596
Alw21I GWGCWC 2 cut(s) 393, 501
Alw26I GTCTC 2 cut(s) 101, 279
AlwI GGATC 2 cut(s) 189, 202
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 4 cut(s) 117, 214, 253, 546
ApoI RAATTY 2 cut(s) 11, 21
AspLEI GCGC 2 cut(s) 563, 638
AspS9I GGNCC 4 cut(s) 4, 80, 214, 355
AsuC2I CCSGG 2 cut(s) 84, 323
AsuHPI GGTGA 5 cut(s) 123, 178, 194, 367, 435
AvaII GGWCC 3 cut(s) 4, 80, 355
AxyI CCTNAGG 1 cut(s) 97
BalI TGGCCA 1 cut(s) 119
BamHI GGATCC 1 cut(s) 194
BanII GRGCYC 2 cut(s) 89, 501
Bbv12I GWGCWC 2 cut(s) 393, 501
BccI CCATC 3 cut(s) 136, 225, 733
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 2 cut(s) 286, 698
BclI TGATCA 2 cut(s) 55, 372
BcnI CCSGG 2 cut(s) 84, 323
BcoDI GTCTC 2 cut(s) 101, 279
BfaI CTAG 1 cut(s) 602
BfmI CTRYAG 1 cut(s) 16
BfoI RGCGCY 1 cut(s) 564
BlpI GCTNAGC 1 cut(s) 557
Bme1390I CCNGG 4 cut(s) 84, 286, 323, 698
Bme18I GGWCC 3 cut(s) 4, 80, 355
BmgBI CACGTC 3 cut(s) 187, 583, 649
BmgT120I GGNCC 4 cut(s) 4, 80, 214, 355
BmiI GGNNCC 3 cut(s) 6, 196, 357
BmrFI CCNGG 4 cut(s) 84, 286, 323, 698
BmsI GCATC 1 cut(s) 596
Bpu1102I GCTNAGC 1 cut(s) 557
BpuMI CCSGG 2 cut(s) 84, 323
Bsa29I ATCGAT 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 4 cut(s) 284, 285, 312, 697
BsaXI ACNNNNNCTCC 4 cut(s) 413, 443, 757, 787
Bsc4I CCNNNNNNNGG 8 cut(s) 33, 98, 99, 141, 234, 269, 270, 570
Bse21I CCTNAGG 1 cut(s) 97
BseBI CCWGG 2 cut(s) 286, 698
BseCI ATCGAT 1 cut(s) 298
BseDI CCNNGG 4 cut(s) 284, 285, 312, 697
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 8 cut(s) 33, 98, 99, 141, 234, 269, 270, 570
BseMII CTCAG 1 cut(s) 111
BseRI GAGGAG 3 cut(s) 85, 119, 509
BseYI CCCAGC 1 cut(s) 43
BsgI GTGCAG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 4 cut(s) 119, 216, 255, 548
BshVI ATCGAT 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 393, 501
BsiSI CCGG 2 cut(s) 83, 323
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 8 cut(s) 33, 98, 99, 141, 234, 269, 270, 570
BsmAI GTCTC 2 cut(s) 101, 279
BsmFI GGGAC 1 cut(s) 49
BsnI GGCC 4 cut(s) 119, 216, 255, 548
Bso31I GGTCTC 1 cut(s) 279
Bsp1286I GDGCHC 3 cut(s) 89, 393, 501
Bsp143I GATC 4 cut(s) 55, 177, 194, 372
Bsp1720I GCTNAGC 1 cut(s) 557
BspACI CCGC 3 cut(s) 435, 468, 620
BspANI GGCC 4 cut(s) 119, 216, 255, 548
BspCNI CTCAG 1 cut(s) 110
BspDI ATCGAT 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 6, 196, 357
BspPI GGATC 2 cut(s) 189, 202
BspTNI GGTCTC 1 cut(s) 279
BssECI CCNNGG 4 cut(s) 284, 285, 312, 697
BssMI GATC 4 cut(s) 55, 177, 194, 372
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 312
Bst2UI CCWGG 2 cut(s) 286, 698
Bst4CI ACNGT 2 cut(s) 172, 225
Bst6I CTCTTC 1 cut(s) 152
BstACI GRCGYC 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 468, 514
BstDEI CTNAG 3 cut(s) 97, 533, 557
BstF5I GGATG 1 cut(s) 217
BstH2I RGCGCY 1 cut(s) 564
BstHHI GCGC 2 cut(s) 563, 638
BstKTI GATC 4 cut(s) 58, 180, 197, 375
BstMAI GTCTC 2 cut(s) 101, 279
BstMBI GATC 4 cut(s) 55, 177, 194, 372
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 1 cut(s) 593
BstNI CCWGG 2 cut(s) 286, 698
BstSCI CCNGG 4 cut(s) 82, 284, 321, 696
BstSFI CTRYAG 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 298
Bsu36I CCTNAGG 1 cut(s) 97
BsuRI GGCC 4 cut(s) 119, 216, 255, 548
BsuTUI ATCGAT 1 cut(s) 298
BtrI CACGTC 3 cut(s) 187, 583, 649
BtsCI GGATG 1 cut(s) 217
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 3 cut(s) 120, 583, 726
Cac8I GCNNGC 2 cut(s) 468, 514
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 2 cut(s) 563, 638
Cfr13I GGNCC 4 cut(s) 4, 80, 214, 355
ClaI ATCGAT 1 cut(s) 298
Csp6I GTAC 2 cut(s) 173, 250
CviAII CATG 2 cut(s) 64, 439
CviQI GTAC 2 cut(s) 173, 250
DdeI CTNAG 3 cut(s) 97, 533, 557
DpnI GATC 4 cut(s) 57, 179, 196, 374
DpnII GATC 4 cut(s) 55, 177, 194, 372
EaeI YGGCCR 3 cut(s) 117, 253, 546
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 312
Eco24I GRGCYC 2 cut(s) 89, 501
Eco31I GGTCTC 1 cut(s) 279
Eco47I GGWCC 3 cut(s) 4, 80, 355
Eco53kI GAGCTC 1 cut(s) 499
Eco81I CCTNAGG 1 cut(s) 97
EcoICRI GAGCTC 1 cut(s) 499
EcoRII CCWGG 2 cut(s) 284, 696
EcoT14I CCWWGG 1 cut(s) 312
EcoT38I GRGCYC 2 cut(s) 89, 501
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 2 cut(s) 67, 442
FaiI YATR 5 cut(s) 65, 280, 381, 440, 645
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 1 cut(s) 49
FatI CATG 2 cut(s) 63, 438
FauI CCCGC 2 cut(s) 475, 613
FbaI TGATCA 2 cut(s) 55, 372
FblI GTMKAC 1 cut(s) 522
FokI GGATG 1 cut(s) 204
FriOI GRGCYC 2 cut(s) 89, 501
FspBI CTAG 1 cut(s) 602
GlaI GCGC 2 cut(s) 562, 637
GsaI CCCAGC 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 564
HaeIII GGCC 4 cut(s) 119, 216, 255, 548
HapII CCGG 2 cut(s) 83, 323
HhaI GCGC 2 cut(s) 563, 638
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 2 cut(s) 67, 442
Hin6I GCGC 2 cut(s) 561, 636
HinP1I GCGC 2 cut(s) 561, 636
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 3 cut(s) 230, 542, 746
HpaII CCGG 2 cut(s) 83, 323
HphI GGTGA 5 cut(s) 123, 178, 194, 367, 435
Hpy166II GTNNAC 2 cut(s) 355, 523
Hpy188I TCNGA 5 cut(s) 73, 229, 612, 731, 751
Hpy188III TCNNGA 2 cut(s) 370, 602
Hpy8I GTNNAC 2 cut(s) 355, 523
HpyAV CCTTC 1 cut(s) 574
HpyCH4III ACNGT 2 cut(s) 172, 225
HpyCH4IV ACGT 5 cut(s) 68, 186, 551, 582, 648
HpyCH4V TGCA 1 cut(s) 154
HpyF10VI GCNNNNNNNGC 1 cut(s) 593
HpyF3I CTNAG 3 cut(s) 97, 533, 557
HpySE526I ACGT 5 cut(s) 68, 186, 551, 582, 648
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 2 cut(s) 67, 442
HspAI GCGC 2 cut(s) 561, 636
Ksp22I TGATCA 2 cut(s) 55, 372
Kzo9I GATC 4 cut(s) 55, 177, 194, 372
LmnI GCTCC 2 cut(s) 314, 496
LweI GCATC 1 cut(s) 596
MaeI CTAG 1 cut(s) 602
MaeII ACGT 5 cut(s) 68, 186, 551, 582, 648
MaeIII GTNAC 6 cut(s) 166, 182, 441, 536, 578, 705
MalI GATC 4 cut(s) 57, 179, 196, 374
MboI GATC 4 cut(s) 55, 177, 194, 372
MboII GAAGA 2 cut(s) 169, 686
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 3 cut(s) 89, 393, 501
MlsI TGGCCA 1 cut(s) 119
MluCI AATT 3 cut(s) 11, 21, 504
MluNI TGGCCA 1 cut(s) 119
MlyI GAGTC 1 cut(s) 536
MmeI TCCRAC 1 cut(s) 754
Mox20I TGGCCA 1 cut(s) 119
MscI TGGCCA 1 cut(s) 119
MseI TTAA 2 cut(s) 267, 776
MslI CAYNNNNRTG 1 cut(s) 437
Msp20I TGGCCA 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 2 cut(s) 83, 323
MspR9I CCNGG 4 cut(s) 84, 286, 323, 698
MvaI CCWGG 2 cut(s) 286, 698
MwoI GCNNNNNNNGC 1 cut(s) 593
NciI CCSGG 2 cut(s) 84, 323
NdeII GATC 4 cut(s) 55, 177, 194, 372
NlaIII CATG 2 cut(s) 67, 442
NlaIV GGNNCC 3 cut(s) 6, 196, 357
NmeAIII GCCGAG 1 cut(s) 524
NmuCI GTSAC 5 cut(s) 166, 182, 441, 536, 578
PasI CCCWGGG 1 cut(s) 285
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 2 cut(s) 230, 746
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 536
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 2 cut(s) 284, 696
PspFI CCCAGC 1 cut(s) 43
PspGI CCWGG 2 cut(s) 284, 696
PspN4I GGNNCC 3 cut(s) 6, 196, 357
PspPI GGNCC 4 cut(s) 4, 80, 214, 355
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 194
PvuI CGATCG 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 596
RsaI GTAC 2 cut(s) 174, 251
RsaNI GTAC 2 cut(s) 173, 250
RseI CAYNNNNRTG 1 cut(s) 437
SacI GAGCTC 1 cut(s) 501
SalI GTCGAC 1 cut(s) 521
SaqAI TTAA 2 cut(s) 267, 776
Sau3AI GATC 4 cut(s) 55, 177, 194, 372
Sau96I GGNCC 4 cut(s) 4, 80, 214, 355
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 4 cut(s) 84, 286, 323, 698
SduI GDGCHC 3 cut(s) 89, 393, 501
SfaNI GCATC 1 cut(s) 596
SfcI CTRYAG 1 cut(s) 16
SinI GGWCC 3 cut(s) 4, 80, 355
SmiMI CAYNNNNRTG 1 cut(s) 437
Sse9I AATT 3 cut(s) 11, 21, 504
SsiI CCGC 3 cut(s) 435, 468, 620
SspMI CTAG 1 cut(s) 602
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 4 cut(s) 82, 284, 321, 696
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 2 cut(s) 172, 225
TaiI ACGT 5 cut(s) 71, 189, 554, 585, 651
TaqI TCGA 3 cut(s) 298, 522, 590
TasI AATT 3 cut(s) 11, 21, 504
TfiI GAWTC 2 cut(s) 230, 746
Tru1I TTAA 2 cut(s) 267, 776
Tru9I TTAA 2 cut(s) 267, 776
TscAI CASTG 3 cut(s) 127, 583, 726
TseFI GTSAC 5 cut(s) 166, 182, 441, 536, 578
Tsp45I GTSAC 5 cut(s) 166, 182, 441, 536, 578
TspGWI ACGGA 1 cut(s) 778
TspRI CASTG 3 cut(s) 127, 583, 726
VpaK11BI GGWCC 3 cut(s) 4, 80, 355
XapI RAATTY 2 cut(s) 11, 21
XbaI TCTAGA 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 522
XspI CTAG 1 cut(s) 602
ZraI GACGTC 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.