Rroxscaffold_2G00113460
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014034
Physical Location & Seq
Reverse (-)
39076452 .. 39078249
1798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00113460.1

Sequence Viewer

Length: 765 bp
ATGGAGGAGCACCCTGAATTTTACAGAAACCCAGCTTTTAGAGATCCACAAGATGCAGAACCCATCTCAGAGAATGATCCGGACTCGCCGTCCTCCGGTGAGGAGACAAAAGGTGACCACTGCACCATCACCCAAGAGAAGGTGGTTTGGTTGTTCTGTTATAGGAGGGGAGTGCAGAAGAGGGTTGTATCGGTTCCGATCGGCGACGTGGAAGGATCCAAGGGAAAAGGAGATGGATATCCACCCTTCGATTCATGGACTTGGAGGAAGTACGGCCAAAAACCTATCAAAGGGTCTCCATATCCCAGGGGATACTATCGATGTAGTTTTTCCAAGGCTTGTCCGGCGAGAAAACAAGTGGAGAGAAGCTGTTCGGACCCAAGAATGCTCATGATCACCTACGCTTGTGACCACAACCACCCTATGCCCACCACCAAAGGATCCCACCCCGCCGCCTTAACCACCCCTGTAACTGCAACATCACCCATCACCGAACCTGAACTTCCCGACAGGGTTGCACCTAGTGAGGAGCTCACTACCTTCGCAAGCCAAGTCGACCTCGAGATTAGTGGCAACTCAGAGGCGTTGCTTGGCGAATATGGCTGCTGGTTCAATGACCTGGGGACGACAACTGTACTAGAAAGTTCAATTGGCGTAGGACATAGTGACTATGTGGACGTTGATGTGGCAACGAGACTGGAACATGAGGATGAGTATTTGTTTGCTGATTTAGGCGAGTTACCTGAAAGTTCGGTGATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

254

Amino Acids

28.26

Weight (kDa)

4.77

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 84 - 141 1.8e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 555
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 2 cut(s) 450, 453
AclWI GGATC 6 cut(s) 38, 71, 210, 223, 435, 448
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 17
AdeI CACNNNGTG 1 cut(s) 524
AfaI GTAC 2 cut(s) 272, 636
AfiI CCNNNNNNNGG 3 cut(s) 95, 139, 290
AgsI TTSAA 2 cut(s) 613, 648
AhdI GACNNNNNGTC 1 cut(s) 88
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 305, 618
AjuI GAANNNNNNNTTGG 2 cut(s) 633, 665
AluBI AGCT 3 cut(s) 35, 369, 532
AluI AGCT 3 cut(s) 35, 369, 532
Alw21I GWGCWC 2 cut(s) 12, 534
Alw26I GTCTC 3 cut(s) 98, 300, 688
AlwI GGATC 6 cut(s) 38, 71, 210, 223, 435, 448
Ama87I CYCGRG 1 cut(s) 560
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 603
ApoI RAATTY 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 370
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 6 cut(s) 110, 121, 125, 388, 474, 481
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 1 cut(s) 376
BamHI GGATCC 2 cut(s) 215, 440
BanII GRGCYC 1 cut(s) 534
Bbv12I GWGCWC 2 cut(s) 12, 534
BbvI GCAGC 1 cut(s) 590
BccI CCATC 4 cut(s) 71, 134, 227, 494
BceAI ACGGC 2 cut(s) 73, 289
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 2 cut(s) 307, 620
BciVI GTATCC 1 cut(s) 305
BclI TGATCA 1 cut(s) 393
BcoDI GTCTC 3 cut(s) 98, 300, 688
BfaI CTAG 2 cut(s) 522, 638
BfuI GTATCC 1 cut(s) 305
BisI GCNGC 2 cut(s) 453, 604
BlsI GCNGC 2 cut(s) 454, 605
Bme1390I CCNGG 2 cut(s) 307, 620
Bme18I GGWCC 1 cut(s) 376
BmeRI GACNNNNNGTC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 560
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 4 cut(s) 195, 217, 378, 442
BmrFI CCNGG 2 cut(s) 307, 620
BmsI GCATC 1 cut(s) 43
Bsa29I ATCGAT 1 cut(s) 319
BsaBI GATNNNNATC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 5 cut(s) 219, 305, 306, 333, 619
BsaWI WCCGGW 2 cut(s) 79, 95
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 139, 290
Bse1I ACTGG 1 cut(s) 702
Bse8I GATNNNNATC 1 cut(s) 237
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 2 cut(s) 307, 620
BseCI ATCGAT 1 cut(s) 319
BseDI CCNNGG 5 cut(s) 219, 305, 306, 333, 619
BseGI GGATG 1 cut(s) 715
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 3 cut(s) 95, 139, 290
BseMII CTCAG 2 cut(s) 81, 591
BseNI ACTGG 1 cut(s) 702
BseRI GAGGAG 3 cut(s) 20, 116, 542
BseXI GCAGC 1 cut(s) 590
BseYI CCCAGC 1 cut(s) 31
BsgI GTGCAG 2 cut(s) 106, 194
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 276
BshVI ATCGAT 1 cut(s) 319
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 12, 534
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 3 cut(s) 80, 96, 344
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 3 cut(s) 95, 139, 290
BsmAI GTCTC 3 cut(s) 98, 300, 688
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 300
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 12, 534
Bsp13I TCCGGA 1 cut(s) 79
Bsp143I GATC 6 cut(s) 43, 76, 198, 215, 393, 440
BspACI CCGC 2 cut(s) 450, 453
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 2 cut(s) 80, 590
BspDI ATCGAT 1 cut(s) 319
BspEI TCCGGA 1 cut(s) 79
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 4 cut(s) 195, 217, 378, 442
BspPI GGATC 6 cut(s) 38, 71, 210, 223, 435, 448
BspTNI GGTCTC 1 cut(s) 300
BsrI ACTGG 1 cut(s) 702
BssECI CCNNGG 5 cut(s) 219, 305, 306, 333, 619
BssMI GATC 6 cut(s) 43, 76, 198, 215, 393, 440
BssT1I CCWWGG 2 cut(s) 219, 333
Bst2UI CCWGG 2 cut(s) 307, 620
Bst4CI ACNGT 1 cut(s) 634
Bst6I CTCTTC 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 2 cut(s) 67, 577
BstEII GGTNACC 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 288
BstF5I GGATG 1 cut(s) 715
BstKTI GATC 6 cut(s) 46, 79, 201, 218, 396, 443
BstMAI GTCTC 3 cut(s) 98, 300, 688
BstMBI GATC 6 cut(s) 43, 76, 198, 215, 393, 440
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 2 cut(s) 344, 600
BstNI CCWGG 2 cut(s) 307, 620
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 305, 618
BstV1I GCAGC 1 cut(s) 590
BstX2I RGATCY 3 cut(s) 43, 215, 440
BstYI RGATCY 3 cut(s) 43, 215, 440
Bsu15I ATCGAT 1 cut(s) 319
BsuI GTATCC 1 cut(s) 305
BsuRI GGCC 1 cut(s) 276
BsuTUI ATCGAT 1 cut(s) 319
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 715
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 1 cut(s) 547
CciI TCATGA 1 cut(s) 390
Cfr13I GGNCC 1 cut(s) 376
ClaI ATCGAT 1 cut(s) 319
Csp6I GTAC 2 cut(s) 271, 635
CviAII CATG 3 cut(s) 255, 391, 704
CviJI RGCY 7 cut(s) 35, 276, 338, 369, 532, 549, 603
CviKI_1 RGCY 7 cut(s) 35, 276, 338, 369, 532, 549, 603
CviQI GTAC 2 cut(s) 271, 635
DdeI CTNAG 2 cut(s) 67, 577
DpnI GATC 6 cut(s) 45, 78, 200, 217, 395, 442
DpnII GATC 6 cut(s) 43, 76, 198, 215, 393, 440
DraIII CACNNNGTG 1 cut(s) 524
DriI GACNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 173
Eam1105I GACNNNNNGTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 173
Ecl136II GAGCTC 1 cut(s) 532
Eco130I CCWWGG 2 cut(s) 219, 333
Eco24I GRGCYC 1 cut(s) 534
Eco31I GGTCTC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 376
Eco53kI GAGCTC 1 cut(s) 532
Eco88I CYCGRG 1 cut(s) 560
Eco91I GGTNACC 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 532
EcoNI CCTNNNNNAGG 1 cut(s) 288
EcoO65I GGTNACC 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 305, 618
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 2 cut(s) 219, 333
EcoT38I GRGCYC 1 cut(s) 534
ErhI CCWWGG 2 cut(s) 219, 333
FaeI CATG 3 cut(s) 258, 394, 707
FaiI YATR 9 cut(s) 162, 256, 301, 392, 425, 600, 663, 672, 705
FaqI GGGAC 1 cut(s) 637
FatI CATG 3 cut(s) 254, 390, 703
FauI CCCGC 1 cut(s) 457
FbaI TGATCA 1 cut(s) 393
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 2 cut(s) 453, 604
FokI GGATG 1 cut(s) 722
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 2 cut(s) 453, 604
FspBI CTAG 2 cut(s) 522, 638
GluI GCNGC 2 cut(s) 453, 604
GsaI CCCAGC 1 cut(s) 35
HaeIII GGCC 1 cut(s) 276
HapII CCGG 3 cut(s) 80, 96, 344
Hin1II CATG 3 cut(s) 258, 394, 707
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 2 cut(s) 83, 251
HpaII CCGG 3 cut(s) 80, 96, 344
HphI GGTGA 6 cut(s) 110, 121, 125, 388, 474, 481
Hpy166II GTNNAC 2 cut(s) 556, 676
Hpy188I TCNGA 4 cut(s) 70, 198, 376, 580
Hpy188III TCNNGA 4 cut(s) 80, 391, 506, 562
Hpy8I GTNNAC 2 cut(s) 556, 676
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 4 cut(s) 133, 206, 256, 550
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4IV ACGT 2 cut(s) 207, 678
HpyCH4V TGCA 5 cut(s) 56, 123, 175, 476, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 600
HpyF3I CTNAG 2 cut(s) 67, 577
HpySE526I ACGT 2 cut(s) 207, 678
Hsp92II CATG 3 cut(s) 258, 394, 707
Kpn2I TCCGGA 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 6 cut(s) 43, 76, 198, 215, 393, 440
LmnI GCTCC 2 cut(s) 7, 529
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 1 cut(s) 43
MaeI CTAG 2 cut(s) 522, 638
MaeII ACGT 2 cut(s) 207, 678
MaeIII GTNAC 5 cut(s) 113, 407, 469, 665, 738
MalI GATC 6 cut(s) 45, 78, 200, 217, 395, 442
MboI GATC 6 cut(s) 43, 76, 198, 215, 393, 440
MboII GAAGA 1 cut(s) 190
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 3 cut(s) 43, 215, 440
MhlI GDGCHC 2 cut(s) 12, 534
MluCI AATT 2 cut(s) 17, 648
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 9 cut(s) 94, 103, 159, 174, 258, 520, 569, 574, 700
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 370
MseI TTAA 1 cut(s) 458
MslI CAYNNNNRTG 1 cut(s) 708
MspI CCGG 3 cut(s) 80, 96, 344
MspR9I CCNGG 2 cut(s) 307, 620
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 2 cut(s) 307, 620
MwoI GCNNNNNNNGC 2 cut(s) 344, 600
NdeII GATC 6 cut(s) 43, 76, 198, 215, 393, 440
NlaIII CATG 3 cut(s) 258, 394, 707
NlaIV GGNNCC 4 cut(s) 195, 217, 378, 442
NmuCI GTSAC 3 cut(s) 113, 407, 665
PaeR7I CTCGAG 1 cut(s) 560
PagI TCATGA 1 cut(s) 390
PasI CCCWGGG 1 cut(s) 306
PctI GAATGC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 370
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 454, 605
Ple19I CGATCG 1 cut(s) 201
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 305, 618
PspEI GGTNACC 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 31
PspGI CCWGG 2 cut(s) 305, 618
PspN4I GGNNCC 4 cut(s) 195, 217, 378, 442
PspPI GGNCC 1 cut(s) 376
PsuI RGATCY 3 cut(s) 43, 215, 440
PvuI CGATCG 1 cut(s) 201
RsaI GTAC 2 cut(s) 272, 636
RsaNI GTAC 2 cut(s) 271, 635
RseI CAYNNNNRTG 1 cut(s) 708
SacI GAGCTC 1 cut(s) 534
SalI GTCGAC 1 cut(s) 554
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 2 cut(s) 453, 604
Sau3AI GATC 6 cut(s) 43, 76, 198, 215, 393, 440
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 307, 620
SduI GDGCHC 2 cut(s) 12, 534
SfaNI GCATC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 560
SinI GGWCC 1 cut(s) 376
SlaI CTCGAG 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 708
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 2 cut(s) 17, 648
SsiI CCGC 2 cut(s) 450, 453
SspMI CTAG 2 cut(s) 522, 638
SstI GAGCTC 1 cut(s) 534
StyD4I CCNGG 2 cut(s) 305, 618
StyI CCWWGG 2 cut(s) 219, 333
TaaI ACNGT 1 cut(s) 634
TaiI ACGT 2 cut(s) 210, 681
TaqI TCGA 4 cut(s) 249, 319, 555, 561
TasI AATT 2 cut(s) 17, 648
TatI WGTACW 1 cut(s) 634
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 3 cut(s) 113, 407, 665
TseI GCWGC 1 cut(s) 603
Tsp45I GTSAC 3 cut(s) 113, 407, 665
TspDTI ATGAA 1 cut(s) 243
TspRI CASTG 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 376
XagI CCTNNNNNAGG 1 cut(s) 288
XapI RAATTY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 1 cut(s) 370
XspI CTAG 2 cut(s) 522, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.