MD09G1235100.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014034
Physical Location & Seq
Reverse (-)
29539556 .. 29542546
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1235100.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGAGGACTCACCCTCCTCAGCCTCAGAGGACGACACACAGGTGGCCACTCCCCTGCCCAAGAAAAGGAGGGGAGTGCAGAAGAGGGTGGTGTCAGTACCGATCGGTGACGTGGAGGGATCCAAGAGCAAAGGGGAGGGGCATCCACCGTCGGATTCTTGGGCCTGGAGAAAGTACGGCCAAAAGCCCATCAAAGGCTCTCCATATCCCAGGGGATATTATCGATGTAGTAGTTCCAAAGGATGCCCTGCAAGAAAACAAGTGGAGAGAAGCCGTCTGAACCCCACAATGCTCATAGTCACCTATGTTTGTGACCACAACCACCCCATGCCCACCACCAAAGCCAACCAATCCTCCTCTGTAACCCCCACCACCATCACCGCTACCGCTACATCACCCACCACCGAATCCGAACTTCCGGCCAAAACCGAGGAGCTCACAATCTTCACAAGCCAAGTCGACCTTGATCTCAGCGACGACTCGCCCACATTACTTAGCGACTTTGGTTGGTTTAATGATGCGGCATCAACAGTTGTACTGGAAAGTCCAATTTGCTTAGGAAATAGTAGTTGCATGAATAATGATGTGGCGACGAGGTTGAAAGACGAGGACGAGTATTTGTTCGCTGATCTCGGCGAGTTACCCGAGGGTTCAGTGATTTTCCGGCATAGGAATAGGATGGTGGAATCAGACGACCAAAATCGGAGATGTAGTTTAAGTGCGGTGGTTCCCTATTGTAGTAATACAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

251

Amino Acids

27.4

Weight (kDa)

5.47

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 52 - 109 3.5e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 459
AciI CCGC 4 cut(s) 381, 387, 521, 722
AclWI GGATC 2 cut(s) 114, 127
AcoI YGGCCR 3 cut(s) 45, 178, 420
AfaI GTAC 3 cut(s) 99, 176, 537
AfiI CCNNNNNNNGG 2 cut(s) 66, 194
AgsI TTSAA 1 cut(s) 601
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 2 cut(s) 164, 209
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 1 cut(s) 436
AluI AGCT 1 cut(s) 436
Alw21I GWGCWC 1 cut(s) 438
AlwI GGATC 2 cut(s) 114, 127
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 4 cut(s) 45, 162, 178, 420
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 5 cut(s) 3, 119, 292, 370, 387
AvaI CYCGRG 1 cut(s) 644
BalI TGGCCA 1 cut(s) 47
BamHI GGATCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 19
BccI CCATC 3 cut(s) 197, 383, 673
BceAI ACGGC 2 cut(s) 193, 258
BciT130I CCWGG 2 cut(s) 166, 211
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
Bme1390I CCNGG 2 cut(s) 166, 211
BmeT110I CYCGRG 1 cut(s) 644
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 2 cut(s) 121, 729
BmrFI CCNGG 2 cut(s) 166, 211
BmsI GCATC 4 cut(s) 151, 233, 508, 533
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 2 cut(s) 19, 556
Bsa29I ATCGAT 1 cut(s) 223
BsaJI CCNNGG 4 cut(s) 209, 210, 429, 645
BsaXI ACNNNNNCTCC 5 cut(s) 29, 338, 368, 697, 727
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 194
Bse1I ACTGG 1 cut(s) 543
BseBI CCWGG 2 cut(s) 166, 211
BseCI ATCGAT 1 cut(s) 223
BseDI CCNNGG 4 cut(s) 209, 210, 429, 645
BseGI GGATG 3 cut(s) 142, 248, 684
BseLI CCNNNNNNNGG 2 cut(s) 66, 194
BseMII CTCAG 3 cut(s) 33, 39, 484
BseNI ACTGG 1 cut(s) 543
BseRI GAGGAG 3 cut(s) 7, 346, 446
BsgI GTGCAG 1 cut(s) 98
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 47, 164, 180, 422
BshVI ATCGAT 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 2 cut(s) 419, 664
BslI CCNNNNNNNGG 2 cut(s) 66, 194
BsnI GGCC 4 cut(s) 47, 164, 180, 422
BsoBI CYCGRG 1 cut(s) 644
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 4 cut(s) 102, 119, 466, 628
BspACI CCGC 4 cut(s) 381, 387, 521, 722
BspANI GGCC 4 cut(s) 47, 164, 180, 422
BspCNI CTCAG 3 cut(s) 32, 38, 483
BspDI ATCGAT 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 121, 729
BspPI GGATC 2 cut(s) 114, 127
BsrI ACTGG 1 cut(s) 543
BssECI CCNNGG 4 cut(s) 209, 210, 429, 645
BssMI GATC 4 cut(s) 102, 119, 466, 628
Bst2UI CCWGG 2 cut(s) 166, 211
Bst4CI ACNGT 2 cut(s) 150, 532
Bst6I CTCTTC 1 cut(s) 77
BstDEI CTNAG 5 cut(s) 19, 25, 470, 494, 556
BstF5I GGATG 3 cut(s) 142, 248, 684
BstKTI GATC 4 cut(s) 105, 122, 469, 631
BstMBI GATC 4 cut(s) 102, 119, 466, 628
BstMCI CGRYCG 1 cut(s) 105
BstNI CCWGG 2 cut(s) 166, 211
BstSCI CCNGG 2 cut(s) 164, 209
BstX2I RGATCY 1 cut(s) 119
BstYI RGATCY 1 cut(s) 119
Bsu15I ATCGAT 1 cut(s) 223
BsuRI GGCC 4 cut(s) 47, 164, 180, 422
BsuTUI ATCGAT 1 cut(s) 223
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 3 cut(s) 142, 248, 684
BtsIMutI CAGTG 1 cut(s) 660
Cfr13I GGNCC 1 cut(s) 162
ClaI ATCGAT 1 cut(s) 223
Csp6I GTAC 3 cut(s) 98, 175, 536
CviAII CATG 2 cut(s) 328, 574
CviQI GTAC 3 cut(s) 98, 175, 536
DdeI CTNAG 5 cut(s) 19, 25, 470, 494, 556
DpnI GATC 4 cut(s) 104, 121, 468, 630
DpnII GATC 4 cut(s) 102, 119, 466, 628
EaeI YGGCCR 3 cut(s) 45, 178, 420
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 438
Eco53kI GAGCTC 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 436
EcoRII CCWGG 2 cut(s) 164, 209
EcoT38I GRGCYC 1 cut(s) 438
FaeI CATG 2 cut(s) 331, 577
FaiI YATR 6 cut(s) 205, 296, 306, 329, 575, 669
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FatI CATG 2 cut(s) 327, 573
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 3 cut(s) 129, 255, 691
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 1 cut(s) 522
GluI GCNGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 4 cut(s) 47, 164, 180, 422
HapII CCGG 2 cut(s) 419, 664
Hin1II CATG 2 cut(s) 331, 577
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 5 cut(s) 8, 155, 407, 479, 686
HpaII CCGG 2 cut(s) 419, 664
HphI GGTGA 5 cut(s) 3, 119, 292, 370, 387
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 6 cut(s) 28, 154, 279, 412, 691, 705
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 3 cut(s) 154, 479, 595
HpyCH4III ACNGT 2 cut(s) 150, 532
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 3 cut(s) 79, 251, 573
HpyF3I CTNAG 5 cut(s) 19, 25, 470, 494, 556
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 2 cut(s) 331, 577
Kzo9I GATC 4 cut(s) 102, 119, 466, 628
LmnI GCTCC 1 cut(s) 433
LweI GCATC 4 cut(s) 151, 233, 508, 533
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 5 cut(s) 107, 298, 311, 361, 639
MalI GATC 4 cut(s) 104, 121, 468, 630
MboI GATC 4 cut(s) 102, 119, 466, 628
MboII GAAGA 2 cut(s) 94, 436
MflI RGATCY 1 cut(s) 119
MhlI GDGCHC 1 cut(s) 438
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 1 cut(s) 549
MluNI TGGCCA 1 cut(s) 47
MlyI GAGTC 2 cut(s) 2, 473
MmeI TCCRAC 1 cut(s) 132
Mox20I TGGCCA 1 cut(s) 47
MscI TGGCCA 1 cut(s) 47
MseI TTAA 2 cut(s) 513, 716
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 47
MspI CCGG 2 cut(s) 419, 664
MspR9I CCNGG 2 cut(s) 166, 211
MvaI CCWGG 2 cut(s) 166, 211
NdeII GATC 4 cut(s) 102, 119, 466, 628
NlaIII CATG 2 cut(s) 331, 577
NlaIV GGNNCC 2 cut(s) 121, 729
NmeAIII GCCGAG 1 cut(s) 612
NmuCI GTSAC 3 cut(s) 107, 298, 311
OliI CACNNNNGTG 1 cut(s) 41
PasI CCCWGGG 1 cut(s) 210
PfeI GAWTC 3 cut(s) 155, 407, 686
PkrI GCNGC 1 cut(s) 523
Ple19I CGATCG 1 cut(s) 105
PleI GAGTC 2 cut(s) 2, 473
PpsI GAGTC 2 cut(s) 2, 473
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 2 cut(s) 164, 209
PspGI CCWGG 2 cut(s) 164, 209
PspN4I GGNNCC 2 cut(s) 121, 729
PspPI GGNCC 1 cut(s) 162
PsuI RGATCY 1 cut(s) 119
PvuI CGATCG 1 cut(s) 105
RsaI GTAC 3 cut(s) 99, 176, 537
RsaNI GTAC 3 cut(s) 98, 175, 536
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 438
SalI GTCGAC 1 cut(s) 458
SaqAI TTAA 2 cut(s) 513, 716
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 4 cut(s) 102, 119, 466, 628
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 2 cut(s) 2, 473
ScrFI CCNGG 2 cut(s) 166, 211
SduI GDGCHC 1 cut(s) 438
SetI ASST 6 cut(s) 45, 114, 305, 438, 465, 599
SfaNI GCATC 4 cut(s) 151, 233, 508, 533
SmiMI CAYNNNNRTG 1 cut(s) 41
Sse9I AATT 1 cut(s) 549
SsiI CCGC 4 cut(s) 381, 387, 521, 722
SstI GAGCTC 1 cut(s) 438
StyD4I CCNGG 2 cut(s) 164, 209
TaaI ACNGT 2 cut(s) 150, 532
TaiI ACGT 1 cut(s) 114
TaqI TCGA 2 cut(s) 223, 459
TasI AATT 1 cut(s) 549
TatI WGTACW 1 cut(s) 535
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 3 cut(s) 155, 407, 686
Tru1I TTAA 2 cut(s) 513, 716
Tru9I TTAA 2 cut(s) 513, 716
TscAI CASTG 1 cut(s) 660
TseFI GTSAC 3 cut(s) 107, 298, 311
Tsp45I GTSAC 3 cut(s) 107, 298, 311
TspDTI ATGAA 1 cut(s) 590
TspRI CASTG 1 cut(s) 660
XmiI GTMKAC 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.