RchiOBHm_Chr2g0130891
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014034
Physical Location & Seq
Forward (+)
46961380 .. 46962974
1595 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50230

Sequence Viewer

Length: 741 bp
ATGGAGGAGCACCCTAAATTTTACAGAAACCCAGTTTTTAGAGATCCGCAAGATGCAGAACCCATCTCAGAGAATGATCCGGACTCGCCGTCCTCCGGTGAGGAGACAAAGGTGACCACTGCACCATCACCCAAGGGAAGGAGGGGAGTGCAGAAGAGGGTTGTGTCGGTTCCGATCGGCGACGTGGAAGGATCCAAGGGAAAAGGAGATGGCTATCCACCCTTCGATTCATGGACTTGGAGGAAGTACGGCCAAAAACCTATCAAAGGGTCTCCATATCCCAGGGGATACTATCGATGTAGTTTTTCCAAGGCTTGTCCGGCGAGAAAACAAGTGGAGAGAAGCTGTTCAGACCCAAGAATGCTCATGATCACCTACGCTTGTGACCACAACCACCCTATGCCCACCACCAAAGGATCCCACCCCGCCGCCTTAACCACCCCTGTAACTGCAAAATCACCCATCACCGAACCTGAACTTCCCGACAGGGTTGCACCTAGTGAGGAGCTCACAACCTTCTCAAGCCAAGTCGACCTCGAGCTTAGTGGCAACTCAGAGGCGTTGCTTGGCGACTATGGCTGCTGGTTCAATGACCTGGGGACGACAACTGTACTAGAAAGTTCAATTTGCGTAGGACATAGTGACTATGTGGACGTTGATGTGGCAACGAGGCTGGAACATGAGGATGAGTATTTGTTTGCTGATTTAGGCGAGTTACCTGAAAGTTCGGTGATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

246

Amino Acids

27.18

Weight (kDa)

4.97

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 76 - 133 1.7e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 531
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 3 cut(s) 47, 426, 429
AclWI GGATC 6 cut(s) 38, 71, 186, 199, 411, 424
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 1 cut(s) 17
AdeI CACNNNGTG 1 cut(s) 500
AfaI GTAC 2 cut(s) 248, 612
AfiI CCNNNNNNNGG 3 cut(s) 95, 138, 266
AgsI TTSAA 2 cut(s) 589, 624
AhdI GACNNNNNGTC 1 cut(s) 88
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 2 cut(s) 281, 594
AluBI AGCT 3 cut(s) 345, 508, 541
AluI AGCT 3 cut(s) 345, 508, 541
Alw21I GWGCWC 2 cut(s) 12, 510
Alw26I GTCTC 2 cut(s) 98, 276
AlwI GGATC 6 cut(s) 38, 71, 186, 199, 411, 424
Ama87I CYCGRG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 1 cut(s) 579
ApoI RAATTY 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 346
AsuHPI GGTGA 6 cut(s) 110, 120, 124, 364, 450, 457
AvaI CYCGRG 1 cut(s) 536
BamHI GGATCC 2 cut(s) 191, 416
BanII GRGCYC 1 cut(s) 510
Bbv12I GWGCWC 2 cut(s) 12, 510
BbvI GCAGC 1 cut(s) 566
BccI CCATC 4 cut(s) 71, 133, 203, 470
BceAI ACGGC 2 cut(s) 73, 265
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 2 cut(s) 283, 596
BciVI GTATCC 1 cut(s) 281
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 2 cut(s) 98, 276
BfaI CTAG 2 cut(s) 498, 614
BfuI GTATCC 1 cut(s) 281
BisI GCNGC 2 cut(s) 429, 580
BlsI GCNGC 2 cut(s) 430, 581
Bme1390I CCNGG 2 cut(s) 283, 596
BmeRI GACNNNNNGTC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 536
BmgBI CACGTC 1 cut(s) 184
BmiI GGNNCC 3 cut(s) 171, 193, 418
BmrFI CCNGG 2 cut(s) 283, 596
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 505
Bsa29I ATCGAT 1 cut(s) 295
BsaBI GATNNNNATC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 6 cut(s) 132, 195, 281, 282, 309, 595
BsaWI WCCGGW 2 cut(s) 79, 95
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 138, 266
Bse1I ACTGG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 213
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 2 cut(s) 283, 596
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 6 cut(s) 132, 195, 281, 282, 309, 595
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 3 cut(s) 95, 138, 266
BseMII CTCAG 2 cut(s) 81, 567
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 3 cut(s) 20, 116, 518
BseXI GCAGC 1 cut(s) 566
BsgI GTGCAG 2 cut(s) 105, 170
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 252
BshVI ATCGAT 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 12, 510
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 3 cut(s) 80, 96, 320
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 3 cut(s) 95, 138, 266
BsmAI GTCTC 2 cut(s) 98, 276
BsmFI GGGAC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 1 cut(s) 252
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 12, 510
Bsp13I TCCGGA 1 cut(s) 79
Bsp143I GATC 6 cut(s) 43, 76, 174, 191, 369, 416
BspACI CCGC 3 cut(s) 47, 426, 429
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 2 cut(s) 80, 566
BspDI ATCGAT 1 cut(s) 295
BspEI TCCGGA 1 cut(s) 79
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 3 cut(s) 171, 193, 418
BspPI GGATC 6 cut(s) 38, 71, 186, 199, 411, 424
BspTNI GGTCTC 1 cut(s) 276
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 6 cut(s) 132, 195, 281, 282, 309, 595
BssMI GATC 6 cut(s) 43, 76, 174, 191, 369, 416
BssT1I CCWWGG 3 cut(s) 132, 195, 309
Bst2UI CCWGG 2 cut(s) 283, 596
Bst4CI ACNGT 1 cut(s) 610
Bst6I CTCTTC 1 cut(s) 149
BstDEI CTNAG 3 cut(s) 67, 542, 553
BstEII GGTNACC 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 691
BstKTI GATC 6 cut(s) 46, 79, 177, 194, 372, 419
BstMAI GTCTC 2 cut(s) 98, 276
BstMBI GATC 6 cut(s) 43, 76, 174, 191, 369, 416
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 2 cut(s) 320, 576
BstNI CCWGG 2 cut(s) 283, 596
BstPI GGTNACC 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 281, 594
BstV1I GCAGC 1 cut(s) 566
BstX2I RGATCY 3 cut(s) 43, 191, 416
BstYI RGATCY 3 cut(s) 43, 191, 416
Bsu15I ATCGAT 1 cut(s) 295
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 295
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
CciI TCATGA 1 cut(s) 366
ClaI ATCGAT 1 cut(s) 295
Csp6I GTAC 2 cut(s) 247, 611
CviAII CATG 3 cut(s) 231, 367, 680
CviJI RGCY 9 cut(s) 213, 252, 314, 345, 508, 525, 541, 579, 673
CviKI_1 RGCY 9 cut(s) 213, 252, 314, 345, 508, 525, 541, 579, 673
CviQI GTAC 2 cut(s) 247, 611
DdeI CTNAG 3 cut(s) 67, 542, 553
DpnI GATC 6 cut(s) 45, 78, 176, 193, 371, 418
DpnII GATC 6 cut(s) 43, 76, 174, 191, 369, 416
DraIII CACNNNGTG 1 cut(s) 500
DriI GACNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 149
Eam1105I GACNNNNNGTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 3 cut(s) 132, 195, 309
Eco24I GRGCYC 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 508
Eco88I CYCGRG 1 cut(s) 536
Eco91I GGTNACC 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 508
EcoNI CCTNNNNNAGG 1 cut(s) 264
EcoO65I GGTNACC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 281, 594
EcoT14I CCWWGG 3 cut(s) 132, 195, 309
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 3 cut(s) 132, 195, 309
FaeI CATG 3 cut(s) 234, 370, 683
FaiI YATR 8 cut(s) 232, 277, 368, 401, 576, 639, 648, 681
FaqI GGGAC 1 cut(s) 613
FatI CATG 3 cut(s) 230, 366, 679
FauI CCCGC 1 cut(s) 433
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 2 cut(s) 429, 580
FokI GGATG 1 cut(s) 698
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 2 cut(s) 429, 580
FspBI CTAG 2 cut(s) 498, 614
GluI GCNGC 2 cut(s) 429, 580
HaeIII GGCC 1 cut(s) 252
HapII CCGG 3 cut(s) 80, 96, 320
Hin1II CATG 3 cut(s) 234, 370, 683
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HinfI GANTC 2 cut(s) 83, 227
HpaII CCGG 3 cut(s) 80, 96, 320
HphI GGTGA 6 cut(s) 110, 120, 124, 364, 450, 457
Hpy166II GTNNAC 2 cut(s) 532, 652
Hpy188I TCNGA 4 cut(s) 70, 174, 352, 556
Hpy188III TCNNGA 3 cut(s) 80, 367, 482
Hpy8I GTNNAC 2 cut(s) 532, 652
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 4 cut(s) 132, 182, 232, 526
HpyCH4III ACNGT 1 cut(s) 610
HpyCH4IV ACGT 2 cut(s) 183, 654
HpyCH4V TGCA 5 cut(s) 56, 122, 151, 452, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 576
HpyF3I CTNAG 3 cut(s) 67, 542, 553
HpySE526I ACGT 2 cut(s) 183, 654
Hsp92II CATG 3 cut(s) 234, 370, 683
Kpn2I TCCGGA 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 6 cut(s) 43, 76, 174, 191, 369, 416
LmnI GCTCC 2 cut(s) 7, 505
Lsp1109I GCAGC 1 cut(s) 566
LweI GCATC 1 cut(s) 43
MaeI CTAG 2 cut(s) 498, 614
MaeII ACGT 2 cut(s) 183, 654
MaeIII GTNAC 5 cut(s) 112, 383, 445, 641, 714
MalI GATC 6 cut(s) 45, 78, 176, 193, 371, 418
MboI GATC 6 cut(s) 43, 76, 174, 191, 369, 416
MboII GAAGA 1 cut(s) 166
MflI RGATCY 3 cut(s) 43, 191, 416
MhlI GDGCHC 2 cut(s) 12, 510
MluCI AATT 2 cut(s) 17, 624
MlyI GAGTC 1 cut(s) 77
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 346
MseI TTAA 1 cut(s) 434
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 3 cut(s) 80, 96, 320
MspR9I CCNGG 2 cut(s) 283, 596
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 2 cut(s) 283, 596
MwoI GCNNNNNNNGC 2 cut(s) 320, 576
NdeII GATC 6 cut(s) 43, 76, 174, 191, 369, 416
NlaIII CATG 3 cut(s) 234, 370, 683
NlaIV GGNNCC 3 cut(s) 171, 193, 418
NmuCI GTSAC 3 cut(s) 112, 383, 641
PaeR7I CTCGAG 1 cut(s) 536
PagI TCATGA 1 cut(s) 366
PasI CCCWGGG 1 cut(s) 282
PctI GAATGC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 346
PfeI GAWTC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 430, 581
Ple19I CGATCG 1 cut(s) 177
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 2 cut(s) 281, 594
PspEI GGTNACC 1 cut(s) 112
PspGI CCWGG 2 cut(s) 281, 594
PspN4I GGNNCC 3 cut(s) 171, 193, 418
PspXI VCTCGAGB 1 cut(s) 536
PsuI RGATCY 3 cut(s) 43, 191, 416
PvuI CGATCG 1 cut(s) 177
RsaI GTAC 2 cut(s) 248, 612
RsaNI GTAC 2 cut(s) 247, 611
RseI CAYNNNNRTG 1 cut(s) 684
SacI GAGCTC 1 cut(s) 510
SalI GTCGAC 1 cut(s) 530
SaqAI TTAA 1 cut(s) 434
SatI GCNGC 2 cut(s) 429, 580
Sau3AI GATC 6 cut(s) 43, 76, 174, 191, 369, 416
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 283, 596
SduI GDGCHC 2 cut(s) 12, 510
SfaNI GCATC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 536
SlaI CTCGAG 1 cut(s) 536
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 2 cut(s) 520, 536
SmoI CTYRAG 2 cut(s) 520, 536
Sse9I AATT 2 cut(s) 17, 624
SsiI CCGC 3 cut(s) 47, 426, 429
SspMI CTAG 2 cut(s) 498, 614
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 2 cut(s) 281, 594
StyI CCWWGG 3 cut(s) 132, 195, 309
TaaI ACNGT 1 cut(s) 610
TaiI ACGT 2 cut(s) 186, 657
TaqI TCGA 4 cut(s) 225, 295, 531, 537
TasI AATT 2 cut(s) 17, 624
TatI WGTACW 1 cut(s) 610
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 1 cut(s) 227
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 112, 383, 641
TseI GCWGC 1 cut(s) 579
Tsp45I GTSAC 3 cut(s) 112, 383, 641
TspDTI ATGAA 1 cut(s) 219
TspRI CASTG 1 cut(s) 124
XagI CCTNNNNNAGG 1 cut(s) 264
XapI RAATTY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 531
XmnI GAANNNNTTC 1 cut(s) 346
XspI CTAG 2 cut(s) 498, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.