Rw2G027440
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014034
Physical Location & Seq
Reverse (-)
43109808 .. 43111403
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027440.1

Sequence Viewer

Length: 732 bp
ATGGAGGAGCACCCTAAATTTTACAGAAACCCAGTTTTTAGAGATCCGCAAGATGCAGAACCCATCTCAGAGAATGATCCGGACTCGCCGTCCTCCGGTGAGGAGACAAAGGTGACCACTGCACCATCACCCAAGGGAAGTGCAGAAGAGGGTTGTGTCGGTTCCGATCGGCGACGTGGAAGGATCCAAGGGAAAGGAGATGGCTATCCACCCTTCGATTCATGGACTTGGAGGAAGTACGGCCAAAAACCTATCAAAGGGTCTCCATATCCCAGGGGATACTATCGATGTAGTTTTTCCAAGGATTGTCCGGCGAGAAAACAAGTGGAGAGAAGCTGTTCAGACCCAAGAATGCTCATGATCACCTACGCTTGTGACCACAACCACCCTATGCCCACCACCAAAGGATCCCACCCCGCCGCCTTAACCACCCCTGTAACTGCAAAATCACCCATCACCGAACCTGAACTTCCCGACAGGGTTGCACCTAGTGAGGAGCTCACAACCTTCTCAAGCCAAGTCGACCTCGAGCTTAGTGGCAACTCAGAGGCGTTGCTTGGCGACTATGGCTGCTGGTTCAATGACCTGGGGACGACAACTGTACTAGAAAGTTCAATTTGCGTAGGACATAGTGACTATGTGGACGTTGATGTGGCAACGAGGCTGGAACATGAGGATGAGTATTTGTTTGCTGACTTAGGCGAGTTACCTGAAAGTTCGGTGATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

243

Amino Acids

26.93

Weight (kDa)

4.8

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 73 - 130 1e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 522
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 3 cut(s) 47, 417, 420
AclWI GGATC 6 cut(s) 38, 71, 178, 191, 402, 415
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 1 cut(s) 17
AdeI CACNNNGTG 1 cut(s) 491
AfaI GTAC 2 cut(s) 239, 603
AfiI CCNNNNNNNGG 2 cut(s) 95, 257
AgsI TTSAA 2 cut(s) 580, 615
AhdI GACNNNNNGTC 1 cut(s) 88
AjiI CACGTC 1 cut(s) 176
AjnI CCWGG 2 cut(s) 272, 585
AluBI AGCT 3 cut(s) 336, 499, 532
AluI AGCT 3 cut(s) 336, 499, 532
Alw21I GWGCWC 2 cut(s) 12, 501
Alw26I GTCTC 2 cut(s) 98, 267
AlwI GGATC 6 cut(s) 38, 71, 178, 191, 402, 415
Ama87I CYCGRG 1 cut(s) 527
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 1 cut(s) 570
ApoI RAATTY 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 337
AsuHPI GGTGA 6 cut(s) 110, 120, 124, 355, 441, 448
AvaI CYCGRG 1 cut(s) 527
BamHI GGATCC 2 cut(s) 183, 407
BanII GRGCYC 1 cut(s) 501
Bbv12I GWGCWC 2 cut(s) 12, 501
BbvI GCAGC 1 cut(s) 557
BccI CCATC 4 cut(s) 71, 133, 194, 461
BceAI ACGGC 2 cut(s) 73, 256
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 2 cut(s) 274, 587
BciVI GTATCC 1 cut(s) 272
BclI TGATCA 1 cut(s) 360
BcoDI GTCTC 2 cut(s) 98, 267
BfaI CTAG 2 cut(s) 489, 605
BfuI GTATCC 1 cut(s) 272
BisI GCNGC 2 cut(s) 420, 571
BlsI GCNGC 2 cut(s) 421, 572
Bme1390I CCNGG 2 cut(s) 274, 587
BmeRI GACNNNNNGTC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 527
BmgBI CACGTC 1 cut(s) 176
BmiI GGNNCC 3 cut(s) 163, 185, 409
BmrFI CCNGG 2 cut(s) 274, 587
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 496
Bsa29I ATCGAT 1 cut(s) 286
BsaBI GATNNNNATC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 6 cut(s) 132, 187, 272, 273, 300, 586
BsaWI WCCGGW 2 cut(s) 79, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 257
Bse1I ACTGG 1 cut(s) 32
Bse8I GATNNNNATC 1 cut(s) 204
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 2 cut(s) 274, 587
BseCI ATCGAT 1 cut(s) 286
BseDI CCNNGG 6 cut(s) 132, 187, 272, 273, 300, 586
BseGI GGATG 1 cut(s) 682
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 95, 257
BseMII CTCAG 2 cut(s) 81, 558
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 3 cut(s) 20, 116, 509
BseXI GCAGC 1 cut(s) 557
BsgI GTGCAG 2 cut(s) 105, 162
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 1 cut(s) 243
BshVI ATCGAT 1 cut(s) 286
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 2 cut(s) 12, 501
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 3 cut(s) 80, 96, 311
BslFI GGGAC 1 cut(s) 604
BslI CCNNNNNNNGG 2 cut(s) 95, 257
BsmAI GTCTC 2 cut(s) 98, 267
BsmFI GGGAC 1 cut(s) 604
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 1 cut(s) 243
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 527
Bsp1286I GDGCHC 2 cut(s) 12, 501
Bsp13I TCCGGA 1 cut(s) 79
Bsp143I GATC 6 cut(s) 43, 76, 166, 183, 360, 407
BspACI CCGC 3 cut(s) 47, 417, 420
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 2 cut(s) 80, 557
BspDI ATCGAT 1 cut(s) 286
BspEI TCCGGA 1 cut(s) 79
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 3 cut(s) 163, 185, 409
BspPI GGATC 6 cut(s) 38, 71, 178, 191, 402, 415
BspTNI GGTCTC 1 cut(s) 267
BsrI ACTGG 1 cut(s) 32
BssECI CCNNGG 6 cut(s) 132, 187, 272, 273, 300, 586
BssMI GATC 6 cut(s) 43, 76, 166, 183, 360, 407
BssT1I CCWWGG 3 cut(s) 132, 187, 300
Bst2UI CCWGG 2 cut(s) 274, 587
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 1 cut(s) 141
BstDEI CTNAG 4 cut(s) 67, 533, 544, 697
BstEII GGTNACC 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 682
BstKTI GATC 6 cut(s) 46, 79, 169, 186, 363, 410
BstMAI GTCTC 2 cut(s) 98, 267
BstMBI GATC 6 cut(s) 43, 76, 166, 183, 360, 407
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 567
BstNI CCWGG 2 cut(s) 274, 587
BstPI GGTNACC 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 272, 585
BstV1I GCAGC 1 cut(s) 557
BstX2I RGATCY 3 cut(s) 43, 183, 407
BstYI RGATCY 3 cut(s) 43, 183, 407
Bsu15I ATCGAT 1 cut(s) 286
BsuI GTATCC 1 cut(s) 272
BsuRI GGCC 1 cut(s) 243
BsuTUI ATCGAT 1 cut(s) 286
BtrI CACGTC 1 cut(s) 176
BtsCI GGATG 1 cut(s) 682
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
CciI TCATGA 1 cut(s) 357
ClaI ATCGAT 1 cut(s) 286
Csp6I GTAC 2 cut(s) 238, 602
CviAII CATG 3 cut(s) 222, 358, 671
CviJI RGCY 8 cut(s) 204, 243, 336, 499, 516, 532, 570, 664
CviKI_1 RGCY 8 cut(s) 204, 243, 336, 499, 516, 532, 570, 664
CviQI GTAC 2 cut(s) 238, 602
DdeI CTNAG 4 cut(s) 67, 533, 544, 697
DpnI GATC 6 cut(s) 45, 78, 168, 185, 362, 409
DpnII GATC 6 cut(s) 43, 76, 166, 183, 360, 407
DraIII CACNNNGTG 1 cut(s) 491
DriI GACNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 141
Eam1105I GACNNNNNGTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 141
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 3 cut(s) 132, 187, 300
Eco24I GRGCYC 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 499
Eco88I CYCGRG 1 cut(s) 527
Eco91I GGTNACC 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 499
EcoNI CCTNNNNNAGG 1 cut(s) 255
EcoO65I GGTNACC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 272, 585
EcoT14I CCWWGG 3 cut(s) 132, 187, 300
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 3 cut(s) 132, 187, 300
FaeI CATG 3 cut(s) 225, 361, 674
FaiI YATR 8 cut(s) 223, 268, 359, 392, 567, 630, 639, 672
FaqI GGGAC 1 cut(s) 604
FatI CATG 3 cut(s) 221, 357, 670
FauI CCCGC 1 cut(s) 424
FbaI TGATCA 1 cut(s) 360
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 420, 571
FokI GGATG 1 cut(s) 689
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 2 cut(s) 420, 571
FspBI CTAG 2 cut(s) 489, 605
GluI GCNGC 2 cut(s) 420, 571
HaeIII GGCC 1 cut(s) 243
HapII CCGG 3 cut(s) 80, 96, 311
Hin1II CATG 3 cut(s) 225, 361, 674
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 2 cut(s) 83, 218
HpaII CCGG 3 cut(s) 80, 96, 311
HphI GGTGA 6 cut(s) 110, 120, 124, 355, 441, 448
Hpy166II GTNNAC 2 cut(s) 523, 643
Hpy188I TCNGA 4 cut(s) 70, 166, 343, 547
Hpy188III TCNNGA 3 cut(s) 80, 358, 473
Hpy8I GTNNAC 2 cut(s) 523, 643
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 3 cut(s) 174, 223, 517
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 2 cut(s) 175, 645
HpyCH4V TGCA 5 cut(s) 56, 122, 143, 443, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 567
HpyF3I CTNAG 4 cut(s) 67, 533, 544, 697
HpySE526I ACGT 2 cut(s) 175, 645
Hsp92II CATG 3 cut(s) 225, 361, 674
Kpn2I TCCGGA 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 6 cut(s) 43, 76, 166, 183, 360, 407
LmnI GCTCC 2 cut(s) 7, 496
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 1 cut(s) 43
MaeI CTAG 2 cut(s) 489, 605
MaeII ACGT 2 cut(s) 175, 645
MaeIII GTNAC 5 cut(s) 112, 374, 436, 632, 705
MalI GATC 6 cut(s) 45, 78, 168, 185, 362, 409
MboI GATC 6 cut(s) 43, 76, 166, 183, 360, 407
MboII GAAGA 1 cut(s) 158
MflI RGATCY 3 cut(s) 43, 183, 407
MhlI GDGCHC 2 cut(s) 12, 501
MluCI AATT 2 cut(s) 17, 615
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 9 cut(s) 94, 103, 142, 225, 487, 536, 541, 654, 667
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 337
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 3 cut(s) 80, 96, 311
MspR9I CCNGG 2 cut(s) 274, 587
Mva1269I GAATGC 1 cut(s) 357
MvaI CCWGG 2 cut(s) 274, 587
MwoI GCNNNNNNNGC 1 cut(s) 567
NdeII GATC 6 cut(s) 43, 76, 166, 183, 360, 407
NlaIII CATG 3 cut(s) 225, 361, 674
NlaIV GGNNCC 3 cut(s) 163, 185, 409
NmuCI GTSAC 3 cut(s) 112, 374, 632
PaeR7I CTCGAG 1 cut(s) 527
PagI TCATGA 1 cut(s) 357
PasI CCCWGGG 1 cut(s) 273
PctI GAATGC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 218
PkrI GCNGC 2 cut(s) 421, 572
Ple19I CGATCG 1 cut(s) 169
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 2 cut(s) 272, 585
PspEI GGTNACC 1 cut(s) 112
PspGI CCWGG 2 cut(s) 272, 585
PspN4I GGNNCC 3 cut(s) 163, 185, 409
PspXI VCTCGAGB 1 cut(s) 527
PsuI RGATCY 3 cut(s) 43, 183, 407
PvuI CGATCG 1 cut(s) 169
RsaI GTAC 2 cut(s) 239, 603
RsaNI GTAC 2 cut(s) 238, 602
RseI CAYNNNNRTG 1 cut(s) 675
SacI GAGCTC 1 cut(s) 501
SalI GTCGAC 1 cut(s) 521
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 2 cut(s) 420, 571
Sau3AI GATC 6 cut(s) 43, 76, 166, 183, 360, 407
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 274, 587
SduI GDGCHC 2 cut(s) 12, 501
SfaNI GCATC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 527
SlaI CTCGAG 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 2 cut(s) 511, 527
SmoI CTYRAG 2 cut(s) 511, 527
Sse9I AATT 2 cut(s) 17, 615
SsiI CCGC 3 cut(s) 47, 417, 420
SspMI CTAG 2 cut(s) 489, 605
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 2 cut(s) 272, 585
StyI CCWWGG 3 cut(s) 132, 187, 300
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 2 cut(s) 178, 648
TaqI TCGA 4 cut(s) 216, 286, 522, 528
TasI AATT 2 cut(s) 17, 615
TatI WGTACW 1 cut(s) 601
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 112, 374, 632
TseI GCWGC 1 cut(s) 570
Tsp45I GTSAC 3 cut(s) 112, 374, 632
TspDTI ATGAA 1 cut(s) 210
TspRI CASTG 1 cut(s) 124
XagI CCTNNNNNAGG 1 cut(s) 255
XapI RAATTY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 337
XspI CTAG 2 cut(s) 489, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.