Rh2CG332200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0014034
Physical Location & Seq
Forward (+)
43670502 .. 43672607
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG332200.1

Sequence Viewer

Length: 861 bp
ATGGAGGAGCACCCTAAATTTTACAGAAACCCCGTTTTTAGAGATCCACAAGATGCAGAACCCATCTCAGAGAATGATCCGGACTCGCCGTCCTCCGGTGAGGAGACAAAGGTGACCACTGCACCATCACCCAAGGGAAGGTATGCTTTTATTCTTCTTGTTTTTTTTTTTTTCCTTTGGGAAAGCATAAAAGAAAATTATGGGGTTTTATTTTATGAGAAATTGAAGACCGTTAAGGTGGTTTGGTTGTTCTGTTATAGGAGGGGAGTGCAAAAGAGGGTTGTGTCGGTTCCGATCGGCGACGTGGAAGGATCCAAGGGAAAAGGAGATGGATATCCACCCTTCGATTCATGGACTTGGAGGAAGTACGGCCAAAAACCTATCAAAGGGTCTCCATATCCCAGGGGATACTATCGATGTAGTTTTTCCAAGGCTTGTCCGGCGAGAAAACAAGTGGAGAGAAGCTGTTCGGACCCAACAATGCTCATGATCTCCTACGCTTGTGACCACAACCACCCTATGCCCACGACCAAAGGATCCCACCCCGCCGCCTTAACCACCCCTGTAACTGCAAAATCACCCATCACCGAACCTGAACTTCCCGACAGGGTTGCACCTAGTGAGGAGCTCACAACCTTCTCAAGCCAAGTCGACCTCGAGCTTAGTGGCAACTCAGAGGCGTTGCTTGGCGACTATGGCTGCTGGTTCAATGACCTGGGGACGACAACTGTACTAGAAAGTTCAATTTGCGTAGGACATAGTGACTATGTGGACGTTGATGTGGCAACGAGGCTGGAACATGAGGATGAGTATTTGTTTGCTGATTTAGGCGAGTTACCTGAAAGTTCGGTGATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

286

Amino Acids

32.16

Weight (kDa)

5.1

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 116 - 173 1.2e-23 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 651
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 2 cut(s) 546, 549
AclWI GGATC 6 cut(s) 38, 71, 306, 319, 531, 544
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 17
AdeI CACNNNGTG 1 cut(s) 620
AfaI GTAC 2 cut(s) 368, 732
AfiI CCNNNNNNNGG 3 cut(s) 95, 138, 386
AgsI TTSAA 3 cut(s) 226, 709, 744
AhdI GACNNNNNGTC 1 cut(s) 88
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 2 cut(s) 401, 714
AluBI AGCT 3 cut(s) 465, 628, 661
AluI AGCT 3 cut(s) 465, 628, 661
Alw21I GWGCWC 2 cut(s) 12, 630
Alw26I GTCTC 2 cut(s) 98, 396
AlwI GGATC 6 cut(s) 38, 71, 306, 319, 531, 544
Ama87I CYCGRG 1 cut(s) 656
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 1 cut(s) 370
ApeKI GCWGC 1 cut(s) 699
ApoI RAATTY 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 472
AsuHPI GGTGA 5 cut(s) 110, 120, 124, 570, 577
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 1 cut(s) 472
BamHI GGATCC 2 cut(s) 311, 536
BanII GRGCYC 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 2 cut(s) 12, 630
BbvI GCAGC 1 cut(s) 686
BccI CCATC 4 cut(s) 71, 133, 323, 590
BceAI ACGGC 2 cut(s) 73, 385
BcgI CGANNNNNNTGC 2 cut(s) 593, 627
BciT130I CCWGG 2 cut(s) 403, 716
BciVI GTATCC 1 cut(s) 401
BcoDI GTCTC 2 cut(s) 98, 396
BfaI CTAG 2 cut(s) 618, 734
BfuI GTATCC 1 cut(s) 401
BisI GCNGC 2 cut(s) 549, 700
BlsI GCNGC 2 cut(s) 550, 701
Bme1390I CCNGG 2 cut(s) 403, 716
Bme18I GGWCC 1 cut(s) 472
BmeRI GACNNNNNGTC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 656
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 472
BmiI GGNNCC 4 cut(s) 291, 313, 474, 538
BmrFI CCNGG 2 cut(s) 403, 716
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 625
Bsa29I ATCGAT 1 cut(s) 415
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 396
BsaJI CCNNGG 6 cut(s) 132, 315, 401, 402, 429, 715
BsaWI WCCGGW 2 cut(s) 79, 95
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 138, 386
Bse8I GATNNNNATC 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 2 cut(s) 403, 716
BseCI ATCGAT 1 cut(s) 415
BseDI CCNNGG 6 cut(s) 132, 315, 401, 402, 429, 715
BseGI GGATG 1 cut(s) 811
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 3 cut(s) 95, 138, 386
BseMII CTCAG 2 cut(s) 81, 687
BseRI GAGGAG 3 cut(s) 20, 116, 638
BseXI GCAGC 1 cut(s) 686
BsgI GTGCAG 1 cut(s) 105
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 1 cut(s) 372
BshVI ATCGAT 1 cut(s) 415
BsiEI CGRYCG 1 cut(s) 297
BsiHKAI GWGCWC 2 cut(s) 12, 630
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 3 cut(s) 80, 96, 440
BslFI GGGAC 1 cut(s) 733
BslI CCNNNNNNNGG 3 cut(s) 95, 138, 386
BsmAI GTCTC 2 cut(s) 98, 396
BsmFI GGGAC 1 cut(s) 733
BsnI GGCC 1 cut(s) 372
Bso31I GGTCTC 1 cut(s) 396
BsoBI CYCGRG 1 cut(s) 656
Bsp1286I GDGCHC 2 cut(s) 12, 630
Bsp13I TCCGGA 1 cut(s) 79
Bsp143I GATC 6 cut(s) 43, 76, 294, 311, 489, 536
BspACI CCGC 2 cut(s) 546, 549
BspANI GGCC 1 cut(s) 372
BspCNI CTCAG 2 cut(s) 80, 686
BspDI ATCGAT 1 cut(s) 415
BspEI TCCGGA 1 cut(s) 79
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 4 cut(s) 291, 313, 474, 538
BspPI GGATC 6 cut(s) 38, 71, 306, 319, 531, 544
BspTNI GGTCTC 1 cut(s) 396
BssECI CCNNGG 6 cut(s) 132, 315, 401, 402, 429, 715
BssMI GATC 6 cut(s) 43, 76, 294, 311, 489, 536
BssT1I CCWWGG 3 cut(s) 132, 315, 429
Bst2UI CCWGG 2 cut(s) 403, 716
Bst4CI ACNGT 2 cut(s) 232, 730
BstDEI CTNAG 3 cut(s) 67, 662, 673
BstEII GGTNACC 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 811
BstKTI GATC 6 cut(s) 46, 79, 297, 314, 492, 539
BstMAI GTCTC 2 cut(s) 98, 396
BstMBI GATC 6 cut(s) 43, 76, 294, 311, 489, 536
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 2 cut(s) 440, 696
BstNI CCWGG 2 cut(s) 403, 716
BstPI GGTNACC 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 401, 714
BstV1I GCAGC 1 cut(s) 686
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 3 cut(s) 43, 311, 536
BstYI RGATCY 3 cut(s) 43, 311, 536
Bsu15I ATCGAT 1 cut(s) 415
BsuI GTATCC 1 cut(s) 401
BsuRI GGCC 1 cut(s) 372
BsuTUI ATCGAT 1 cut(s) 415
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 1 cut(s) 811
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
CciI TCATGA 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 472
ClaI ATCGAT 1 cut(s) 415
Csp6I GTAC 2 cut(s) 367, 731
CviAII CATG 3 cut(s) 351, 487, 800
CviJI RGCY 8 cut(s) 372, 434, 465, 628, 645, 661, 699, 793
CviKI_1 RGCY 8 cut(s) 372, 434, 465, 628, 645, 661, 699, 793
CviQI GTAC 2 cut(s) 367, 731
DdeI CTNAG 3 cut(s) 67, 662, 673
DpnI GATC 6 cut(s) 45, 78, 296, 313, 491, 538
DpnII GATC 6 cut(s) 43, 76, 294, 311, 489, 536
DraIII CACNNNGTG 1 cut(s) 620
DriI GACNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 370
Eam1105I GACNNNNNGTC 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 3 cut(s) 132, 315, 429
Eco24I GRGCYC 1 cut(s) 630
Eco31I GGTCTC 1 cut(s) 396
Eco32I GATATC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 472
Eco53kI GAGCTC 1 cut(s) 628
Eco88I CYCGRG 1 cut(s) 656
Eco91I GGTNACC 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 628
EcoNI CCTNNNNNAGG 1 cut(s) 384
EcoO65I GGTNACC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 401, 714
EcoRV GATATC 1 cut(s) 335
EcoT14I CCWWGG 3 cut(s) 132, 315, 429
EcoT38I GRGCYC 1 cut(s) 630
ErhI CCWWGG 3 cut(s) 132, 315, 429
FaeI CATG 3 cut(s) 354, 490, 803
FalI AAGNNNNNCTT 2 cut(s) 130, 162
FaqI GGGAC 1 cut(s) 733
FatI CATG 3 cut(s) 350, 486, 799
FauI CCCGC 1 cut(s) 553
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 2 cut(s) 549, 700
FokI GGATG 1 cut(s) 818
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 2 cut(s) 549, 700
FspBI CTAG 2 cut(s) 618, 734
GluI GCNGC 2 cut(s) 549, 700
HaeIII GGCC 1 cut(s) 372
HapII CCGG 3 cut(s) 80, 96, 440
Hin1II CATG 3 cut(s) 354, 490, 803
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 2 cut(s) 83, 347
HpaII CCGG 3 cut(s) 80, 96, 440
HphI GGTGA 5 cut(s) 110, 120, 124, 570, 577
Hpy166II GTNNAC 2 cut(s) 652, 772
Hpy188I TCNGA 4 cut(s) 70, 294, 472, 676
Hpy188III TCNNGA 3 cut(s) 80, 487, 602
Hpy8I GTNNAC 2 cut(s) 652, 772
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 4 cut(s) 132, 302, 352, 646
HpyCH4III ACNGT 2 cut(s) 232, 730
HpyCH4IV ACGT 2 cut(s) 303, 774
HpyCH4V TGCA 5 cut(s) 56, 122, 271, 572, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 696
HpyF3I CTNAG 3 cut(s) 67, 662, 673
HpySE526I ACGT 2 cut(s) 303, 774
Hsp92II CATG 3 cut(s) 354, 490, 803
Kpn2I TCCGGA 1 cut(s) 79
Kzo9I GATC 6 cut(s) 43, 76, 294, 311, 489, 536
LmnI GCTCC 2 cut(s) 7, 625
Lsp1109I GCAGC 1 cut(s) 686
LweI GCATC 1 cut(s) 43
MaeI CTAG 2 cut(s) 618, 734
MaeII ACGT 2 cut(s) 303, 774
MaeIII GTNAC 5 cut(s) 112, 503, 565, 761, 834
MalI GATC 6 cut(s) 45, 78, 296, 313, 491, 538
MboI GATC 6 cut(s) 43, 76, 294, 311, 489, 536
MboII GAAGA 2 cut(s) 146, 238
MflI RGATCY 3 cut(s) 43, 311, 536
MhlI GDGCHC 2 cut(s) 12, 630
MluCI AATT 4 cut(s) 17, 196, 221, 744
MlyI GAGTC 1 cut(s) 77
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 2 cut(s) 234, 554
MslI CAYNNNNRTG 1 cut(s) 804
MspI CCGG 3 cut(s) 80, 96, 440
MspR9I CCNGG 2 cut(s) 403, 716
MvaI CCWGG 2 cut(s) 403, 716
MwoI GCNNNNNNNGC 2 cut(s) 440, 696
NdeII GATC 6 cut(s) 43, 76, 294, 311, 489, 536
NlaIII CATG 3 cut(s) 354, 490, 803
NlaIV GGNNCC 4 cut(s) 291, 313, 474, 538
NmuCI GTSAC 3 cut(s) 112, 503, 761
PaeR7I CTCGAG 1 cut(s) 656
PagI TCATGA 1 cut(s) 486
PasI CCCWGGG 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 1 cut(s) 347
PkrI GCNGC 2 cut(s) 550, 701
Ple19I CGATCG 1 cut(s) 297
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 630
Psp6I CCWGG 2 cut(s) 401, 714
PspEI GGTNACC 1 cut(s) 112
PspGI CCWGG 2 cut(s) 401, 714
PspN4I GGNNCC 4 cut(s) 291, 313, 474, 538
PspPI GGNCC 1 cut(s) 472
PspXI VCTCGAGB 1 cut(s) 656
PsuI RGATCY 3 cut(s) 43, 311, 536
PvuI CGATCG 1 cut(s) 297
RsaI GTAC 2 cut(s) 368, 732
RsaNI GTAC 2 cut(s) 367, 731
RseI CAYNNNNRTG 1 cut(s) 804
SacI GAGCTC 1 cut(s) 630
SalI GTCGAC 1 cut(s) 650
SaqAI TTAA 2 cut(s) 234, 554
SatI GCNGC 2 cut(s) 549, 700
Sau3AI GATC 6 cut(s) 43, 76, 294, 311, 489, 536
Sau96I GGNCC 1 cut(s) 472
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 403, 716
SduI GDGCHC 2 cut(s) 12, 630
SfaNI GCATC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 656
SinI GGWCC 1 cut(s) 472
SlaI CTCGAG 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 804
SmlI CTYRAG 2 cut(s) 640, 656
SmoI CTYRAG 2 cut(s) 640, 656
Sse9I AATT 4 cut(s) 17, 196, 221, 744
SsiI CCGC 2 cut(s) 546, 549
SspMI CTAG 2 cut(s) 618, 734
SstI GAGCTC 1 cut(s) 630
StyD4I CCNGG 2 cut(s) 401, 714
StyI CCWWGG 3 cut(s) 132, 315, 429
TaaI ACNGT 2 cut(s) 232, 730
TaiI ACGT 2 cut(s) 306, 777
TaqI TCGA 4 cut(s) 345, 415, 651, 657
TasI AATT 4 cut(s) 17, 196, 221, 744
TatI WGTACW 1 cut(s) 730
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 2 cut(s) 234, 554
Tru9I TTAA 2 cut(s) 234, 554
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 112, 503, 761
TseI GCWGC 1 cut(s) 699
Tsp45I GTSAC 3 cut(s) 112, 503, 761
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 472
XagI CCTNNNNNAGG 1 cut(s) 384
XapI RAATTY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 2 cut(s) 618, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.