Rh2AG345200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014034
Physical Location & Seq
Forward (+)
50745175 .. 50747266
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG345200.1

Sequence Viewer

Length: 858 bp
ATGGAGGAGCACCCTAAATTTTACAGAAACACAGTTTTTAGAGATCCACAAGATGCAGAACCCATCTCAGAGAATGATCCGGACTCGCCGTCCTCCGGTGAGGAGACAAAGGTGACCACTGCACCATCACCCAAGGGAAGGTATGCTTTTATTCTTCTTGTTTTTTTTTTCCTTTGGGAAAGCATAAAAGAAAATTATGGGGTTTTATTTTATGAGAAATTGATGACCGTTAAGGTGGTTTGGTTGTTCTGTTATAGGAGGGGAGTGCAGAAGAAGGTTGTGTCAGTTCCGATCGGCGACGTGGAAGGATCCAAGGGAAAAGGAGATGGATATCCACCCTTCGATTCATGGACTTGGAGGAAGTACGGCCAAAAACCTATCAAAGGGTCTCCATATCCCAGGGGATACTATCGATGTAGTTTTTCCAAGGCTTGTCCGGCGAGAAAACAAGTGGAGAGAAGCTGTTCGGACCCAAGAATGCTCATGATCACCTACGCTTGTGACCACAACCACCCTATGCCCACCACCAAAGGATCCCACCCCGCCGCCTTAACCACACCTGCAACTGCAAAATCACCCATCACCGAACCTGAACTTCCCGACAGGGTTGCACCTAGTGAGGAGCTCACAACCTTCTCAAGCCAAGTCGACCTCGAGCTTAGTGGCAACTCAGAGGCGTTGCTTGGCGACTATGGCTGCTGGTTCAATGACCTGGGGACGACAACTGTACTAGAAAGTTCAATTTGCGTAGGACATAGTGACTATGTGGACGTTGATGTGGCAACGAGGCTGGAACATGAGGATGAGTATTTGTTTGCTGATTTAGGCGAGTTACCTGAAAGTTCGGTGATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

285

Amino Acids

32.03

Weight (kDa)

5.1

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 115 - 172 2.1e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58710 AT3G58710
fragaria_vesca FvH4_6g27740 FvH4_6g27740
malus_domestica MD09G1235100.v1.1
prunus_persica Prupe.3G113300_v2.0.a1
pyrus_communis pycom09g15330
rosa_chinensis RchiOBHm_Chr2g0130891
rosa_laevigata RLG00000019157
rosa_roxburghii Rroxscaffold_2G00113460
rosa_rugosa Rorug02G0293900
rosa_samantha Rh2AG345200 Rh2BG353600 Rh2CG332200 Rh2DG371600
rosa_wichuraiana Rw2G027440 Rw2G028060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 568
Acc36I ACCTGC 1 cut(s) 568
AccI GTMKAC 1 cut(s) 648
AccIII TCCGGA 1 cut(s) 79
AciI CCGC 2 cut(s) 543, 546
AclWI GGATC 6 cut(s) 38, 71, 303, 316, 528, 541
AcoI YGGCCR 1 cut(s) 367
AcsI RAATTY 1 cut(s) 17
AdeI CACNNNGTG 1 cut(s) 617
AfaI GTAC 2 cut(s) 365, 729
AfiI CCNNNNNNNGG 3 cut(s) 95, 138, 383
AgsI TTSAA 2 cut(s) 706, 741
AhdI GACNNNNNGTC 1 cut(s) 88
AjiI CACGTC 1 cut(s) 301
AjnI CCWGG 2 cut(s) 398, 711
AluBI AGCT 3 cut(s) 462, 625, 658
AluI AGCT 3 cut(s) 462, 625, 658
Alw21I GWGCWC 2 cut(s) 12, 627
Alw26I GTCTC 2 cut(s) 98, 393
AlwI GGATC 6 cut(s) 38, 71, 303, 316, 528, 541
Ama87I CYCGRG 1 cut(s) 653
Aor13HI TCCGGA 1 cut(s) 79
AoxI GGCC 1 cut(s) 367
ApeKI GCWGC 1 cut(s) 696
ApoI RAATTY 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 469
AsuHPI GGTGA 6 cut(s) 110, 120, 124, 481, 567, 574
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 1 cut(s) 469
BamHI GGATCC 2 cut(s) 308, 533
BanII GRGCYC 1 cut(s) 627
Bbv12I GWGCWC 2 cut(s) 12, 627
BbvI GCAGC 1 cut(s) 683
BccI CCATC 4 cut(s) 71, 133, 320, 587
BceAI ACGGC 2 cut(s) 73, 382
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 2 cut(s) 400, 713
BciVI GTATCC 1 cut(s) 398
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 2 cut(s) 98, 393
BfaI CTAG 2 cut(s) 615, 731
BfuAI ACCTGC 1 cut(s) 568
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 2 cut(s) 546, 697
BlsI GCNGC 2 cut(s) 547, 698
Bme1390I CCNGG 2 cut(s) 400, 713
Bme18I GGWCC 1 cut(s) 469
BmeRI GACNNNNNGTC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 653
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 3 cut(s) 310, 471, 535
BmrFI CCNGG 2 cut(s) 400, 713
BmsI GCATC 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 622
Bsa29I ATCGAT 1 cut(s) 412
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 6 cut(s) 132, 312, 398, 399, 426, 712
BsaWI WCCGGW 2 cut(s) 79, 95
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 138, 383
Bse8I GATNNNNATC 1 cut(s) 330
BseAI TCCGGA 1 cut(s) 79
BseBI CCWGG 2 cut(s) 400, 713
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 6 cut(s) 132, 312, 398, 399, 426, 712
BseGI GGATG 1 cut(s) 808
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 95, 138, 383
BseMII CTCAG 2 cut(s) 81, 684
BseRI GAGGAG 3 cut(s) 20, 116, 635
BseXI GCAGC 1 cut(s) 683
BsgI GTGCAG 2 cut(s) 105, 287
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 1 cut(s) 369
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 294
BsiHKAI GWGCWC 2 cut(s) 12, 627
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 3 cut(s) 80, 96, 437
BslFI GGGAC 1 cut(s) 730
BslI CCNNNNNNNGG 3 cut(s) 95, 138, 383
BsmAI GTCTC 2 cut(s) 98, 393
BsmFI GGGAC 1 cut(s) 730
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 1 cut(s) 369
Bso31I GGTCTC 1 cut(s) 393
BsoBI CYCGRG 1 cut(s) 653
Bsp1286I GDGCHC 2 cut(s) 12, 627
Bsp13I TCCGGA 1 cut(s) 79
Bsp143I GATC 6 cut(s) 43, 76, 291, 308, 486, 533
BspACI CCGC 2 cut(s) 543, 546
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 2 cut(s) 80, 683
BspDI ATCGAT 1 cut(s) 412
BspEI TCCGGA 1 cut(s) 79
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 3 cut(s) 310, 471, 535
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 6 cut(s) 38, 71, 303, 316, 528, 541
BspTNI GGTCTC 1 cut(s) 393
BssECI CCNNGG 6 cut(s) 132, 312, 398, 399, 426, 712
BssMI GATC 6 cut(s) 43, 76, 291, 308, 486, 533
BssT1I CCWWGG 3 cut(s) 132, 312, 426
Bst2UI CCWGG 2 cut(s) 400, 713
Bst4CI ACNGT 3 cut(s) 34, 229, 727
BstDEI CTNAG 3 cut(s) 67, 659, 670
BstEII GGTNACC 1 cut(s) 112
BstENI CCTNNNNNAGG 1 cut(s) 381
BstF5I GGATG 1 cut(s) 808
BstKTI GATC 6 cut(s) 46, 79, 294, 311, 489, 536
BstMAI GTCTC 2 cut(s) 98, 393
BstMBI GATC 6 cut(s) 43, 76, 291, 308, 486, 533
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 2 cut(s) 437, 693
BstNI CCWGG 2 cut(s) 400, 713
BstPI GGTNACC 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 398, 711
BstV1I GCAGC 1 cut(s) 683
BstX2I RGATCY 3 cut(s) 43, 308, 533
BstYI RGATCY 3 cut(s) 43, 308, 533
Bsu15I ATCGAT 1 cut(s) 412
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 1 cut(s) 369
BsuTUI ATCGAT 1 cut(s) 412
BtrI CACGTC 1 cut(s) 301
BtsCI GGATG 1 cut(s) 808
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
BveI ACCTGC 1 cut(s) 568
CciI TCATGA 1 cut(s) 483
Cfr13I GGNCC 1 cut(s) 469
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 2 cut(s) 364, 728
CviAII CATG 3 cut(s) 348, 484, 797
CviJI RGCY 8 cut(s) 369, 431, 462, 625, 642, 658, 696, 790
CviKI_1 RGCY 8 cut(s) 369, 431, 462, 625, 642, 658, 696, 790
CviQI GTAC 2 cut(s) 364, 728
DdeI CTNAG 3 cut(s) 67, 659, 670
DpnI GATC 6 cut(s) 45, 78, 293, 310, 488, 535
DpnII GATC 6 cut(s) 43, 76, 291, 308, 486, 533
DraIII CACNNNGTG 1 cut(s) 617
DriI GACNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 367
Eam1105I GACNNNNNGTC 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 625
Eco130I CCWWGG 3 cut(s) 132, 312, 426
Eco24I GRGCYC 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 625
Eco88I CYCGRG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 112
EcoICRI GAGCTC 1 cut(s) 625
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoO65I GGTNACC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 398, 711
EcoRV GATATC 1 cut(s) 332
EcoT14I CCWWGG 3 cut(s) 132, 312, 426
EcoT38I GRGCYC 1 cut(s) 627
ErhI CCWWGG 3 cut(s) 132, 312, 426
FaeI CATG 3 cut(s) 351, 487, 800
FalI AAGNNNNNCTT 2 cut(s) 130, 162
FaqI GGGAC 1 cut(s) 730
FatI CATG 3 cut(s) 347, 483, 796
FauI CCCGC 1 cut(s) 550
FbaI TGATCA 1 cut(s) 486
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 2 cut(s) 546, 697
FokI GGATG 1 cut(s) 815
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 2 cut(s) 546, 697
FspBI CTAG 2 cut(s) 615, 731
GluI GCNGC 2 cut(s) 546, 697
HaeIII GGCC 1 cut(s) 369
HapII CCGG 3 cut(s) 80, 96, 437
Hin1II CATG 3 cut(s) 351, 487, 800
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 2 cut(s) 83, 344
HpaII CCGG 3 cut(s) 80, 96, 437
HphI GGTGA 6 cut(s) 110, 120, 124, 481, 567, 574
Hpy166II GTNNAC 2 cut(s) 649, 769
Hpy188I TCNGA 4 cut(s) 70, 291, 469, 673
Hpy188III TCNNGA 3 cut(s) 80, 484, 599
Hpy8I GTNNAC 2 cut(s) 649, 769
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 5 cut(s) 132, 268, 299, 349, 643
HpyCH4III ACNGT 3 cut(s) 34, 229, 727
HpyCH4IV ACGT 2 cut(s) 300, 771
HpyCH4V TGCA 6 cut(s) 56, 122, 268, 563, 569, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 693
HpyF3I CTNAG 3 cut(s) 67, 659, 670
HpySE526I ACGT 2 cut(s) 300, 771
Hsp92II CATG 3 cut(s) 351, 487, 800
Kpn2I TCCGGA 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 6 cut(s) 43, 76, 291, 308, 486, 533
LmnI GCTCC 2 cut(s) 7, 622
Lsp1109I GCAGC 1 cut(s) 683
LweI GCATC 1 cut(s) 43
MaeI CTAG 2 cut(s) 615, 731
MaeII ACGT 2 cut(s) 300, 771
MaeIII GTNAC 4 cut(s) 112, 500, 758, 831
MalI GATC 6 cut(s) 45, 78, 293, 310, 488, 535
MboI GATC 6 cut(s) 43, 76, 291, 308, 486, 533
MboII GAAGA 2 cut(s) 146, 283
MflI RGATCY 3 cut(s) 43, 308, 533
MhlI GDGCHC 2 cut(s) 12, 627
MluCI AATT 4 cut(s) 17, 193, 218, 741
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 9 cut(s) 94, 103, 252, 351, 613, 662, 667, 780, 793
MroI TCCGGA 1 cut(s) 79
MroXI GAANNNNTTC 1 cut(s) 463
MseI TTAA 2 cut(s) 231, 551
MslI CAYNNNNRTG 1 cut(s) 801
MspI CCGG 3 cut(s) 80, 96, 437
MspR9I CCNGG 2 cut(s) 400, 713
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 2 cut(s) 400, 713
MwoI GCNNNNNNNGC 2 cut(s) 437, 693
NdeII GATC 6 cut(s) 43, 76, 291, 308, 486, 533
NlaIII CATG 3 cut(s) 351, 487, 800
NlaIV GGNNCC 3 cut(s) 310, 471, 535
NmuCI GTSAC 3 cut(s) 112, 500, 758
PaeR7I CTCGAG 1 cut(s) 653
PagI TCATGA 1 cut(s) 483
PaqCI CACCTGC 1 cut(s) 568
PasI CCCWGGG 1 cut(s) 399
PctI GAATGC 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 463
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 2 cut(s) 547, 698
Ple19I CGATCG 1 cut(s) 294
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 398, 711
PspEI GGTNACC 1 cut(s) 112
PspGI CCWGG 2 cut(s) 398, 711
PspN4I GGNNCC 3 cut(s) 310, 471, 535
PspPI GGNCC 1 cut(s) 469
PspXI VCTCGAGB 1 cut(s) 653
PsuI RGATCY 3 cut(s) 43, 308, 533
PvuI CGATCG 1 cut(s) 294
RsaI GTAC 2 cut(s) 365, 729
RsaNI GTAC 2 cut(s) 364, 728
RseI CAYNNNNRTG 1 cut(s) 801
SacI GAGCTC 1 cut(s) 627
SalI GTCGAC 1 cut(s) 647
SaqAI TTAA 2 cut(s) 231, 551
SatI GCNGC 2 cut(s) 546, 697
Sau3AI GATC 6 cut(s) 43, 76, 291, 308, 486, 533
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 400, 713
SduI GDGCHC 2 cut(s) 12, 627
SfaNI GCATC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 653
SinI GGWCC 1 cut(s) 469
SlaI CTCGAG 1 cut(s) 653
SmiMI CAYNNNNRTG 1 cut(s) 801
SmlI CTYRAG 2 cut(s) 637, 653
SmoI CTYRAG 2 cut(s) 637, 653
Sse9I AATT 4 cut(s) 17, 193, 218, 741
SsiI CCGC 2 cut(s) 543, 546
SspMI CTAG 2 cut(s) 615, 731
SstI GAGCTC 1 cut(s) 627
StyD4I CCNGG 2 cut(s) 398, 711
StyI CCWWGG 3 cut(s) 132, 312, 426
TaaI ACNGT 3 cut(s) 34, 229, 727
TaiI ACGT 2 cut(s) 303, 774
TaqI TCGA 4 cut(s) 342, 412, 648, 654
TasI AATT 4 cut(s) 17, 193, 218, 741
TatI WGTACW 1 cut(s) 727
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 2 cut(s) 231, 551
Tru9I TTAA 2 cut(s) 231, 551
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 3 cut(s) 112, 500, 758
TseI GCWGC 1 cut(s) 696
Tsp45I GTSAC 3 cut(s) 112, 500, 758
TspDTI ATGAA 1 cut(s) 336
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 469
XagI CCTNNNNNAGG 1 cut(s) 381
XapI RAATTY 1 cut(s) 17
XhoI CTCGAG 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 648
XmnI GAANNNNTTC 1 cut(s) 463
XspI CTAG 2 cut(s) 615, 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.