FvH4_6g28190

Belongs to the TUB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007280
Physical Location & Seq
Forward (+)
21710601 .. 21715951
5351 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28190.t4

Sequence Viewer

Length: 1245 bp
ATGATGAGTGGCTGCAAGAGAACGGCGTTAATGCCGCGGCAATCGTCGTACAATGCCCTCTACGTCAACCCCGACCAGAAGCACAACCGCAGCTTCAGCGAGGACATCCGCAGCAGCCCAAGTGACCATTCCAAACCAGACCACTTGGCTTCGGCTTCAAACGACGACGAAGACAAAGAGAACTCAACGCCAAACAACAACAGCAATGACAAGGAGAACTTGCTGTCGAAGCCACTGACGAAGCAGAATCTGAACAAGTCCTTCTCCACCGGCTGCGGCGGAGGCGGAGGTGGGGCCGCCTTGAAGCCTTCGTCGCTTCAGTTCTGTATGCAGATGAGTGAGCCGGAGAAGGAGCTCTTTGGTGGGTCCAAGATGTGGGACCCGCCCAGCGTGGCGTCGGACAACTCGAGCTCTTTGAAGATTTGGGACTATTCGGATTCTGAGGCTGCTCCTGCTTCTTCTTGGTCCACTCTGCCTAATAAGTCTTTGTTGTGTAGGCCATTACCTATGGATGTAGGAAGATGCACTTGTGTTATAGTGAAGGAAAAATCTCCAGATGGGGGCTTTCTGTATTCTCTCTACACTAATGAAGGCAAAGGAAGGCAGGACAGGAAACTAGCTGTTGCACGCCATAAGCGACGTAATGGAAAATCAGAGTTAACTATTGCTCAAAATATGAAGGGAATATTGTCACATTCTGATGATAGTTTTATTGGGATGGTAAATGCTAATCTCATGGGCTCAAAGTACCACATATGGGATCAGGGGAAGAGACCAAATTGTTTGAGCAAGCAGTCTAAATCACTTCTTGCTGCTGTATCGTTCACACCCACAATTTCAACTTGGACAGGAAGTTACAGACGTATAAGGGCATATGTACCCAAGCACCAATCGATGCAGTTAAAAAATACTGCTCAGGTACAACACATCAAGGGACTGCCTACAGACTGGGAGGCAAAAATGGACAAAATCCATCAGCTATACTCAAGGGTCCCAGCTTACAATAAAATTTCAAAGCAATACGAGTTGGACTTCAGAGATAGGGGAAGAGCTGGGCTTAGAATCCAAAGTTCAGTCAAGAATTTTCAGCTGACACTGGAGGAGAACGGAAAGCAGACAATCCTGCAGCTAGGAAGGGTTGGGAAGTCAAAATATGTAATGGATTACAGGTATCCTTTGACAGGCTACCAGGCATTTTGCATTTGTCTGGCGTCAATTGATTCAAAGCTTTGTTGCTCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tub

Protein Analysis

415

Amino Acids

46.16

Weight (kDa)

9.39

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 164 - 394 3.4e-39 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 375
AccII CGCG 1 cut(s) 37
AciI CCGC 9 cut(s) 35, 37, 88, 109, 276, 279, 285, 297, 383
AclWI GGATC 1 cut(s) 768
AcsI RAATTY 2 cut(s) 1008, 1081
AcuI CTGAAG 3 cut(s) 79, 302, 1018
AcyI GRCGYC 2 cut(s) 395, 1211
AfaI GTAC 4 cut(s) 50, 749, 879, 921
AfiI CCNNNNNNNGG 4 cut(s) 375, 560, 757, 1181
AgsI TTSAA 6 cut(s) 159, 304, 418, 840, 1014, 1224
AjnI CCWGG 1 cut(s) 1188
AjuI GAANNNNNNNTTGG 2 cut(s) 112, 144
Alw21I GWGCWC 2 cut(s) 357, 413
Alw26I GTCTC 1 cut(s) 766
AlwI GGATC 1 cut(s) 768
AlwNI CAGNNNCTG 1 cut(s) 250
Ama87I CYCGRG 1 cut(s) 406
AoxI GGCC 2 cut(s) 294, 497
ApeKI GCWGC 8 cut(s) 12, 90, 111, 114, 273, 446, 812, 1126
ApoI RAATTY 2 cut(s) 1008, 1081
AspS9I GGNCC 5 cut(s) 294, 366, 379, 465, 991
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 4 cut(s) 366, 379, 465, 991
BanII GRGCYC 3 cut(s) 357, 413, 743
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 357, 413
BbvI GCAGC 7 cut(s) 102, 123, 126, 260, 433, 799, 1138
BccI CCATC 3 cut(s) 551, 712, 981
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 1190
BciVI GTATCC 1 cut(s) 1182
BcoDI GTCTC 1 cut(s) 766
BfaI CTAG 2 cut(s) 617, 1130
BfmI CTRYAG 2 cut(s) 942, 1124
BfuI GTATCC 1 cut(s) 1182
Bme1390I CCNGG 1 cut(s) 1190
Bme18I GGWCC 4 cut(s) 366, 379, 465, 991
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 5 cut(s) 294, 366, 379, 465, 991
BmiI GGNNCC 6 cut(s) 295, 367, 380, 381, 992, 993
BmrFI CCNGG 1 cut(s) 1190
BmrI ACTGGG 1 cut(s) 958
BmsI GCATC 2 cut(s) 512, 885
BmuI ACTGGG 1 cut(s) 958
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 2 cut(s) 537, 1118
Bpu10I CCTNAGC 1 cut(s) 915
BpuEI CTTGAG 1 cut(s) 970
Bsa29I ATCGAT 1 cut(s) 893
BsaHI GRCGYC 2 cut(s) 395, 1211
BsaI GGTCTC 1 cut(s) 766
BsaJI CCNNGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 4 cut(s) 375, 560, 757, 1181
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 953, 1101
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 1190
BseCI ATCGAT 1 cut(s) 893
BseDI CCNNGG 1 cut(s) 35
BseGI GGATG 3 cut(s) 105, 517, 723
BseLI CCNNNNNNNGG 4 cut(s) 375, 560, 757, 1181
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 432, 929
BseNI ACTGG 2 cut(s) 953, 1101
BseRI GAGGAG 1 cut(s) 1115
BseXI GCAGC 7 cut(s) 102, 123, 126, 260, 433, 799, 1138
BseYI CCCAGC 3 cut(s) 386, 994, 1052
Bsh1236I CGCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 296, 499
BshVI ATCGAT 1 cut(s) 893
BsiHKAI GWGCWC 2 cut(s) 357, 413
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 2 cut(s) 270, 344
BslFI GGGAC 4 cut(s) 392, 440, 948, 977
BslI CCNNNNNNNGG 4 cut(s) 375, 560, 757, 1181
BsmAI GTCTC 1 cut(s) 766
BsmFI GGGAC 4 cut(s) 392, 440, 948, 977
BsnI GGCC 2 cut(s) 296, 499
Bso31I GGTCTC 1 cut(s) 766
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 3 cut(s) 357, 413, 743
Bsp143I GATC 1 cut(s) 760
BspACI CCGC 9 cut(s) 35, 37, 88, 109, 276, 279, 285, 297, 383
BspANI GGCC 2 cut(s) 296, 499
BspCNI CTCAG 2 cut(s) 433, 928
BspDI ATCGAT 1 cut(s) 893
BspFNI CGCG 1 cut(s) 37
BspLI GGNNCC 6 cut(s) 295, 367, 380, 381, 992, 993
BspMAI CTGCAG 1 cut(s) 1128
BspPI GGATC 1 cut(s) 768
BspQI GCTCTTC 1 cut(s) 1042
BspTNI GGTCTC 1 cut(s) 766
BsrDI GCAATG 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 2 cut(s) 953, 1101
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 35
BssMI GATC 1 cut(s) 760
BssNI GRCGYC 2 cut(s) 395, 1211
Bst2UI CCWGG 1 cut(s) 1190
Bst6I CTCTTC 2 cut(s) 764, 1042
BstACI GRCGYC 2 cut(s) 395, 1211
BstC8I GCNNGC 2 cut(s) 628, 791
BstDEI CTNAG 4 cut(s) 441, 915, 1058, 1237
BstDSI CCRYGG 1 cut(s) 35
BstENI CCTNNNNNAGG 1 cut(s) 1179
BstF5I GGATG 3 cut(s) 105, 517, 723
BstFNI CGCG 1 cut(s) 37
BstKTI GATC 1 cut(s) 763
BstMAI GTCTC 1 cut(s) 766
BstMBI GATC 1 cut(s) 760
BstMWI GCNNNNNNNGC 5 cut(s) 96, 229, 282, 313, 452
BstNI CCWGG 1 cut(s) 1190
BstSCI CCNGG 1 cut(s) 1188
BstSFI CTRYAG 2 cut(s) 942, 1124
BstUI CGCG 1 cut(s) 37
BstV1I GCAGC 7 cut(s) 102, 123, 126, 260, 433, 799, 1138
BstV2I GAAGAC 1 cut(s) 177
Bsu15I ATCGAT 1 cut(s) 893
BsuI GTATCC 1 cut(s) 1182
BsuRI GGCC 2 cut(s) 296, 499
BsuTUI ATCGAT 1 cut(s) 893
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 3 cut(s) 105, 517, 723
BtsIMutI CAGTG 2 cut(s) 233, 1094
Cac8I GCNNGC 2 cut(s) 628, 791
CaiI CAGNNNCTG 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 5 cut(s) 294, 366, 379, 465, 991
Cfr42I CCGCGG 1 cut(s) 38
ClaI ATCGAT 1 cut(s) 893
CseI GACGC 2 cut(s) 384, 1200
Csp6I GTAC 4 cut(s) 49, 748, 878, 920
CviAII CATG 1 cut(s) 736
CviQI GTAC 4 cut(s) 49, 748, 878, 920
DdeI CTNAG 4 cut(s) 441, 915, 1058, 1237
DpnI GATC 1 cut(s) 762
DpnII GATC 1 cut(s) 760
Eam1104I CTCTTC 2 cut(s) 764, 1042
EarI CTCTTC 2 cut(s) 764, 1042
EciI GGCGGA 2 cut(s) 294, 300
Ecl136II GAGCTC 2 cut(s) 355, 411
Eco24I GRGCYC 3 cut(s) 357, 413, 743
Eco31I GGTCTC 1 cut(s) 766
Eco47I GGWCC 4 cut(s) 366, 379, 465, 991
Eco53kI GAGCTC 2 cut(s) 355, 411
Eco57I CTGAAG 3 cut(s) 79, 302, 1018
Eco88I CYCGRG 1 cut(s) 406
EcoICRI GAGCTC 2 cut(s) 355, 411
EcoNI CCTNNNNNAGG 1 cut(s) 1179
EcoO109I RGGNCCY 2 cut(s) 379, 991
EcoRII CCWGG 1 cut(s) 1188
EcoT38I GRGCYC 3 cut(s) 357, 413, 743
FaeI CATG 1 cut(s) 739
FalI AAGNNNNNCTT 6 cut(s) 203, 235, 341, 373, 511, 543
FaqI GGGAC 4 cut(s) 392, 440, 948, 977
FatI CATG 1 cut(s) 735
FauI CCCGC 1 cut(s) 390
FauNDI CATATG 2 cut(s) 755, 874
FokI GGATG 3 cut(s) 92, 524, 730
FriOI GRGCYC 3 cut(s) 357, 413, 743
FspBI CTAG 2 cut(s) 617, 1130
GsaI CCCAGC 3 cut(s) 390, 998, 1056
GsuI CTGGAG 2 cut(s) 537, 1118
HaeIII GGCC 2 cut(s) 296, 499
HapII CCGG 2 cut(s) 270, 344
HgaI GACGC 2 cut(s) 384, 1200
Hin1I GRCGYC 2 cut(s) 395, 1211
Hin1II CATG 1 cut(s) 739
HincII GTYRAC 2 cut(s) 67, 660
HindII GTYRAC 2 cut(s) 67, 660
HindIII AAGCTT 1 cut(s) 1226
HinfI GANTC 4 cut(s) 247, 437, 1062, 1220
HpaI GTTAAC 1 cut(s) 660
HpaII CCGG 2 cut(s) 270, 344
Hpy166II GTNNAC 4 cut(s) 67, 468, 660, 825
Hpy188I TCNGA 7 cut(s) 252, 400, 436, 442, 655, 700, 1037
Hpy188III TCNNGA 2 cut(s) 554, 1078
Hpy8I GTNNAC 4 cut(s) 67, 468, 660, 825
Hpy99I CGWCG 6 cut(s) 49, 167, 170, 316, 400, 642
HpyAV CCTTC 8 cut(s) 271, 318, 343, 535, 584, 594, 673, 1128
HpyCH4IV ACGT 3 cut(s) 63, 640, 862
HpyCH4V TGCA 7 cut(s) 15, 331, 525, 626, 898, 1126, 1200
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 229, 282, 313, 452
HpyF3I CTNAG 4 cut(s) 441, 915, 1058, 1237
HpySE526I ACGT 3 cut(s) 63, 640, 862
Hsp92I GRCGYC 2 cut(s) 395, 1211
Hsp92II CATG 1 cut(s) 739
KflI GGGWCCC 2 cut(s) 379, 991
KspAI GTTAAC 1 cut(s) 660
KspI CCGCGG 1 cut(s) 38
Kzo9I GATC 1 cut(s) 760
LguI GCTCTTC 1 cut(s) 1042
LmnI GCTCC 2 cut(s) 352, 454
Lsp1109I GCAGC 7 cut(s) 102, 123, 126, 260, 433, 799, 1138
LweI GCATC 2 cut(s) 512, 885
MaeI CTAG 2 cut(s) 617, 1130
MaeII ACGT 3 cut(s) 63, 640, 862
MaeIII GTNAC 3 cut(s) 122, 690, 854
MalI GATC 1 cut(s) 762
MboI GATC 1 cut(s) 760
MboII GAAGA 6 cut(s) 182, 430, 450, 531, 781, 1059
MfeI CAATTG 1 cut(s) 1215
MhlI GDGCHC 3 cut(s) 357, 413, 743
MluCI AATT 5 cut(s) 778, 834, 1008, 1081, 1215
MmeI TCCRAC 2 cut(s) 378, 1008
MnlI CCTC 7 cut(s) 68, 94, 275, 281, 436, 946, 1093
MseI TTAA 3 cut(s) 29, 659, 902
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 2 cut(s) 37, 1090
MspI CCGG 2 cut(s) 270, 344
MspR9I CCNGG 1 cut(s) 1190
MunI CAATTG 1 cut(s) 1215
MvaI CCWGG 1 cut(s) 1190
MvnI CGCG 1 cut(s) 37
MwoI GCNNNNNNNGC 5 cut(s) 96, 229, 282, 313, 452
NdeI CATATG 2 cut(s) 755, 874
NdeII GATC 1 cut(s) 760
NlaIII CATG 1 cut(s) 739
NlaIV GGNNCC 6 cut(s) 295, 367, 380, 381, 992, 993
NmuCI GTSAC 2 cut(s) 122, 690
PaeR7I CTCGAG 1 cut(s) 406
PciSI GCTCTTC 1 cut(s) 1042
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 404, 634
PfeI GAWTC 4 cut(s) 247, 437, 1062, 1220
PflMI CCANNNNNTGG 1 cut(s) 375
PpuMI RGGWCCY 2 cut(s) 379, 991
Psp124BI GAGCTC 2 cut(s) 357, 413
Psp5II RGGWCCY 2 cut(s) 379, 991
Psp6I CCWGG 1 cut(s) 1188
PspFI CCCAGC 3 cut(s) 386, 994, 1052
PspGI CCWGG 1 cut(s) 1188
PspN4I GGNNCC 6 cut(s) 295, 367, 380, 381, 992, 993
PspPI GGNCC 5 cut(s) 294, 366, 379, 465, 991
PspPPI RGGWCCY 2 cut(s) 379, 991
PspXI VCTCGAGB 1 cut(s) 406
PstI CTGCAG 1 cut(s) 1128
PstNI CAGNNNCTG 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 1090
RsaI GTAC 4 cut(s) 50, 749, 879, 921
RsaNI GTAC 4 cut(s) 49, 748, 878, 920
RseI CAYNNNNRTG 1 cut(s) 699
SacI GAGCTC 2 cut(s) 357, 413
SacII CCGCGG 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 1042
SaqAI TTAA 3 cut(s) 29, 659, 902
Sau3AI GATC 1 cut(s) 760
Sau96I GGNCC 5 cut(s) 294, 366, 379, 465, 991
ScrFI CCNGG 1 cut(s) 1190
SduI GDGCHC 3 cut(s) 357, 413, 743
SfaNI GCATC 2 cut(s) 512, 885
SfcI CTRYAG 2 cut(s) 942, 1124
Sfr274I CTCGAG 1 cut(s) 406
Sfr303I CCGCGG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 38
SinI GGWCC 4 cut(s) 366, 379, 465, 991
SlaI CTCGAG 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 2 cut(s) 406, 985
SmoI CTYRAG 2 cut(s) 406, 985
Sse9I AATT 5 cut(s) 778, 834, 1008, 1081, 1215
SsiI CCGC 9 cut(s) 35, 37, 88, 109, 276, 279, 285, 297, 383
SspI AATATT 1 cut(s) 687
SspMI CTAG 2 cut(s) 617, 1130
SstI GAGCTC 2 cut(s) 357, 413
StyD4I CCNGG 1 cut(s) 1188
TaiI ACGT 3 cut(s) 66, 643, 865
TaqI TCGA 3 cut(s) 227, 407, 893
TasI AATT 5 cut(s) 778, 834, 1008, 1081, 1215
TauI GCSGC 4 cut(s) 37, 40, 279, 299
TfiI GAWTC 4 cut(s) 247, 437, 1062, 1220
Tru1I TTAA 3 cut(s) 29, 659, 902
Tru9I TTAA 3 cut(s) 29, 659, 902
TscAI CASTG 2 cut(s) 240, 1101
TseFI GTSAC 2 cut(s) 122, 690
TseI GCWGC 8 cut(s) 12, 90, 111, 114, 273, 446, 812, 1126
Tsp45I GTSAC 2 cut(s) 122, 690
TspDTI ATGAA 2 cut(s) 603, 692
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 2 cut(s) 240, 1101
Van91I CCANNNNNTGG 1 cut(s) 375
VpaK11BI GGWCC 4 cut(s) 366, 379, 465, 991
XagI CCTNNNNNAGG 1 cut(s) 1179
XapI RAATTY 2 cut(s) 1008, 1081
XhoI CTCGAG 1 cut(s) 406
XspI CTAG 2 cut(s) 617, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.