MD17G1227000.v1.1

Belongs to the TUB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007280
Physical Location & Seq
Reverse (-)
27503353 .. 27506338
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1227000.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGATGGCTGGTTGCAAGAAAACCACATCAATTCTACAGCAATCCTCATACAGCTCTCTCTATGTCGATCCCCTCACATACCAGAAACACAGCCGCAGCCACAGCCAAGGCATCGCCTTCATCTCCAAGATGGACAATTCGGAGCCGAACCCATCAGCCTTAAACGTCAACAAAGAGAACGCAATGCCCAAGAAGCCACAGAAGGCCGTGCCCGACAGCGACAAGGAGAATTTGGTCGCACATGAACATGGGTCTTCCTCTTTTGCGATGCAGCCATTGAAGCAGAGGAACTTGAAGTCTCTGTCCACTGGCTGCAGGGCCCTGAAGCCCTCATCGCTTCAGTTCTGTATGCAGGCGAACGAGCAGGAGAAGGCACTCTTCGGTGGGTCCACAATGCAGGACCCGCCCAGTAATGGGTCCGACAACTCGAGCTCTTTGAACATCTGGGACTACTCCGACTCTGAGGCTGCCCCTGCCTCCTCTTGGTCTACACTGCCTAACAAGTCATTGCTTTGCAGGCCATTGCCAATGGATGTGGGAAGATGCACATGTGTGATAGTGAAGGAAGCAACCCCGGAAGCAATAGATGGGGGTACTCTCTATTCTCTTTATACCAATGAAGGCAAGGGAAGGCAGGACCGGAAACTAGCTATTGCTCACCATAAGCGGCGGAATGGGAAATCTGAGTTGACAATTGCTCAGAATGTGAAGGGAATACTGTCTCATTCGGATGATAGTTTTATTGGTATTGTAAATGCTAACCTCATGGGTTCAAAATACCACATATGGGATCAGGGTGGTCGTCCCAATTCCTTGAGCAAAGAGGCTAAATCACTTGCCGTTGTAGCGTTCACGCCAACAATCTCGAGCTGGACAGGAAGTTACAGACGTATAAGGGCATATATACCCAAGCACCAATCCATGCAGTTAAAGAACACAACGCAGGTACAACACATTGAGGGACTCCCAAAGGACTGGGAAGGGAAGCTTGACAAAGTTCATCAGCTATCCTCAAGGGTTCCCCATTACAATAAAATTTCAAAGCAGTATGAGCTAGATTTCAGAGACAGGGGAAGAGCGGGTCTTAGAATCCAAAGTTCAGTCAAGAATTTCCAACTGACACTGGAGGAGAACGGAAAGCAGGCAATTCTGCAGCTCGGAAGGGTTGGAAAATCAAAGTATGTGATGGATTACAGATATCCTTTGACAGGCTACCAAGCGTTTTGCATATGCTTGGCTTCTATTGATTCAAAGCTTTGTTGCACTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tub

Protein Analysis

424

Amino Acids

46.96

Weight (kDa)

9.39

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 171 - 417 2e-47 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 934
AccBSI CCGCTC 1 cut(s) 1079
AccI GTMKAC 1 cut(s) 488
AciI CCGC 5 cut(s) 94, 404, 667, 670, 1079
AclWI GGATC 2 cut(s) 62, 798
AcsI RAATTY 3 cut(s) 229, 1035, 1108
AcuI CTGAAG 2 cut(s) 323, 344
AfaI GTAC 2 cut(s) 595, 948
AfiI CCNNNNNNNGG 5 cut(s) 413, 414, 483, 787, 1208
AflIII ACRYGT 1 cut(s) 548
AgsI TTSAA 6 cut(s) 280, 295, 439, 774, 1041, 1251
AleI CACNNNNGTG 1 cut(s) 551
AluBI AGCT 9 cut(s) 54, 432, 650, 870, 988, 1006, 1054, 1156, 1255
AluI AGCT 9 cut(s) 54, 432, 650, 870, 988, 1006, 1054, 1156, 1255
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 3 cut(s) 303, 726, 1059
AlwI GGATC 2 cut(s) 62, 798
Ama87I CYCGRG 2 cut(s) 427, 865
AoxI GGCC 3 cut(s) 204, 318, 518
ApaI GGGCCC 1 cut(s) 322
ApeKI GCWGC 5 cut(s) 96, 271, 312, 467, 1153
ApoI RAATTY 3 cut(s) 229, 1035, 1108
AspS9I GGNCC 6 cut(s) 318, 319, 387, 400, 417, 637
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 650
AvaI CYCGRG 2 cut(s) 427, 865
AvaII GGWCC 4 cut(s) 387, 400, 417, 637
BaeGI GKGCMC 2 cut(s) 213, 322
BanII GRGCYC 2 cut(s) 322, 434
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 5 cut(s) 108, 283, 299, 454, 1165
BccI CCATC 4 cut(s) 124, 160, 581, 1180
BceAI ACGGC 2 cut(s) 191, 824
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 3 cut(s) 303, 726, 1059
BfaI CTAG 3 cut(s) 647, 1055, 1270
BfmI CTRYAG 3 cut(s) 35, 313, 1151
BfuAI ACCTGC 1 cut(s) 934
BisI GCNGC 7 cut(s) 94, 97, 272, 313, 468, 668, 1154
BlsI GCNGC 7 cut(s) 95, 98, 273, 314, 469, 669, 1155
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 4 cut(s) 387, 400, 417, 637
BmeT110I CYCGRG 2 cut(s) 427, 865
BmgT120I GGNCC 6 cut(s) 318, 319, 387, 400, 417, 637
BmiI GGNNCC 6 cut(s) 144, 320, 388, 402, 418, 1020
BmrFI CCNGG 1 cut(s) 575
BmrI ACTGGG 2 cut(s) 402, 985
BmsI GCATC 3 cut(s) 120, 258, 533
BmuI ACTGGG 2 cut(s) 402, 985
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 1145
BpuEI CTTGAG 2 cut(s) 835, 997
BpuMI CCSGG 1 cut(s) 575
BsaJI CCNNGG 2 cut(s) 106, 573
BsaWI WCCGGW 1 cut(s) 639
Bsc4I CCNNNNNNNGG 5 cut(s) 413, 414, 483, 787, 1208
Bse1I ACTGG 4 cut(s) 313, 408, 980, 1128
Bse3DI GCAATG 3 cut(s) 189, 506, 521
BseDI CCNNGG 2 cut(s) 106, 573
BseGI GGATG 2 cut(s) 538, 736
BseLI CCNNNNNNNGG 5 cut(s) 413, 414, 483, 787, 1208
BseMI GCAATG 3 cut(s) 189, 506, 521
BseMII CTCAG 3 cut(s) 453, 675, 713
BseNI ACTGG 4 cut(s) 313, 408, 980, 1128
BseRI GAGGAG 2 cut(s) 469, 1142
BseSI GKGCMC 2 cut(s) 213, 322
BseXI GCAGC 5 cut(s) 108, 283, 299, 454, 1165
BshFI GGCC 3 cut(s) 206, 320, 520
BsiHKAI GWGCWC 1 cut(s) 434
BsiHKCI CYCGRG 2 cut(s) 427, 865
BsiSI CCGG 2 cut(s) 575, 640
BslFI GGGAC 3 cut(s) 461, 789, 975
BslI CCNNNNNNNGG 5 cut(s) 413, 414, 483, 787, 1208
BsmAI GTCTC 3 cut(s) 303, 726, 1059
BsmFI GGGAC 3 cut(s) 461, 789, 975
BsnI GGCC 3 cut(s) 206, 320, 520
BsoBI CYCGRG 2 cut(s) 427, 865
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 213, 322, 434
Bsp143I GATC 2 cut(s) 67, 790
BspACI CCGC 5 cut(s) 94, 404, 667, 670, 1079
BspANI GGCC 3 cut(s) 206, 320, 520
BspCNI CTCAG 3 cut(s) 454, 676, 712
BspLI GGNNCC 6 cut(s) 144, 320, 388, 402, 418, 1020
BspMAI CTGCAG 2 cut(s) 317, 1155
BspMI ACCTGC 1 cut(s) 934
BspPI GGATC 2 cut(s) 62, 798
BspQI GCTCTTC 1 cut(s) 1069
BsrBI CCGCTC 1 cut(s) 1079
BsrDI GCAATG 3 cut(s) 189, 506, 521
BsrI ACTGG 4 cut(s) 313, 408, 980, 1128
BssECI CCNNGG 2 cut(s) 106, 573
BssMI GATC 2 cut(s) 67, 790
BssT1I CCWWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 720, 1267
Bst6I CTCTTC 2 cut(s) 383, 1069
BstC8I GCNNGC 3 cut(s) 354, 518, 1143
BstDEI CTNAG 4 cut(s) 462, 684, 699, 1085
BstENI CCTNNNNNAGG 1 cut(s) 1206
BstF5I GGATG 2 cut(s) 538, 736
BstKTI GATC 2 cut(s) 70, 793
BstMAI GTCTC 3 cut(s) 303, 726, 1059
BstMBI GATC 2 cut(s) 67, 790
BstMWI GCNNNNNNNGC 9 cut(s) 102, 193, 280, 334, 403, 473, 517, 845, 1051
BstNSI RCATGY 1 cut(s) 552
BstSCI CCNGG 1 cut(s) 573
BstSFI CTRYAG 3 cut(s) 35, 313, 1151
BstSLI GKGCMC 2 cut(s) 213, 322
BstV1I GCAGC 5 cut(s) 108, 283, 299, 454, 1165
BstV2I GAAGAC 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 975
BsuRI GGCC 3 cut(s) 206, 320, 520
BtgZI GCGATG 3 cut(s) 97, 281, 318
BtsCI GGATG 2 cut(s) 538, 736
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 4 cut(s) 306, 491, 1121, 1263
BveI ACCTGC 1 cut(s) 934
Cac8I GCNNGC 3 cut(s) 354, 518, 1143
Cfr13I GGNCC 6 cut(s) 318, 319, 387, 400, 417, 637
Csp6I GTAC 2 cut(s) 594, 947
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 5 cut(s) 242, 248, 549, 766, 922
CviQI GTAC 2 cut(s) 594, 947
DdeI CTNAG 4 cut(s) 462, 684, 699, 1085
DpnI GATC 2 cut(s) 69, 792
DpnII GATC 2 cut(s) 67, 790
Eam1104I CTCTTC 2 cut(s) 383, 1069
EarI CTCTTC 2 cut(s) 383, 1069
EciI GGCGGA 1 cut(s) 685
Ecl136II GAGCTC 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 106
Eco24I GRGCYC 2 cut(s) 322, 434
Eco32I GATATC 1 cut(s) 1199
Eco47I GGWCC 4 cut(s) 387, 400, 417, 637
Eco53kI GAGCTC 1 cut(s) 432
Eco57I CTGAAG 2 cut(s) 323, 344
Eco88I CYCGRG 2 cut(s) 427, 865
EcoICRI GAGCTC 1 cut(s) 432
EcoNI CCTNNNNNAGG 1 cut(s) 1206
EcoO109I RGGNCCY 3 cut(s) 318, 319, 400
EcoRV GATATC 1 cut(s) 1199
EcoT14I CCWWGG 1 cut(s) 106
EcoT38I GRGCYC 2 cut(s) 322, 434
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 5 cut(s) 245, 251, 552, 769, 925
FalI AAGNNNNNCTT 4 cut(s) 362, 394, 972, 1004
FaqI GGGAC 3 cut(s) 461, 789, 975
FatI CATG 5 cut(s) 241, 247, 548, 765, 921
FauI CCCGC 2 cut(s) 411, 1072
FauNDI CATATG 2 cut(s) 785, 1229
FblI GTMKAC 1 cut(s) 488
Fnu4HI GCNGC 7 cut(s) 94, 97, 272, 313, 468, 668, 1154
FokI GGATG 2 cut(s) 545, 743
FriOI GRGCYC 2 cut(s) 322, 434
Fsp4HI GCNGC 7 cut(s) 94, 97, 272, 313, 468, 668, 1154
FspBI CTAG 3 cut(s) 647, 1055, 1270
GluI GCNGC 7 cut(s) 94, 97, 272, 313, 468, 668, 1154
GsuI CTGGAG 1 cut(s) 1145
HaeIII GGCC 3 cut(s) 206, 320, 520
HapII CCGG 2 cut(s) 575, 640
Hin1II CATG 5 cut(s) 245, 251, 552, 769, 925
HincII GTYRAC 2 cut(s) 169, 690
HindII GTYRAC 2 cut(s) 169, 690
HindIII AAGCTT 2 cut(s) 986, 1253
HinfI GANTC 4 cut(s) 458, 963, 1089, 1247
HpaII CCGG 2 cut(s) 575, 640
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 6 cut(s) 169, 306, 390, 489, 690, 852
Hpy188I TCNGA 9 cut(s) 142, 421, 457, 463, 685, 702, 730, 1064, 1160
Hpy188III TCNNGA 2 cut(s) 865, 1105
Hpy8I GTNNAC 6 cut(s) 169, 306, 390, 489, 690, 852
HpyAV CCTTC 9 cut(s) 127, 196, 364, 556, 614, 624, 703, 974, 1155
HpyCH4III ACNGT 2 cut(s) 720, 1267
HpyCH4IV ACGT 2 cut(s) 165, 889
HpyF10VI GCNNNNNNNGC 9 cut(s) 102, 193, 280, 334, 403, 473, 517, 845, 1051
HpyF3I CTNAG 4 cut(s) 462, 684, 699, 1085
HpySE526I ACGT 2 cut(s) 165, 889
Hsp92II CATG 5 cut(s) 245, 251, 552, 769, 925
Kzo9I GATC 2 cut(s) 67, 790
LguI GCTCTTC 1 cut(s) 1069
LmnI GCTCC 1 cut(s) 142
Lsp1109I GCAGC 5 cut(s) 108, 283, 299, 454, 1165
LweI GCATC 3 cut(s) 120, 258, 533
MaeI CTAG 3 cut(s) 647, 1055, 1270
MaeII ACGT 2 cut(s) 165, 889
MaeIII GTNAC 1 cut(s) 881
MalI GATC 2 cut(s) 69, 792
MbiI CCGCTC 1 cut(s) 1079
MboI GATC 2 cut(s) 67, 790
MboII GAAGA 4 cut(s) 246, 370, 552, 1086
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 3 cut(s) 213, 322, 434
MluCI AATT 8 cut(s) 30, 136, 229, 693, 808, 1035, 1108, 1146
MlyI GAGTC 2 cut(s) 452, 957
MmeI TCCRAC 4 cut(s) 444, 480, 1138, 1147
MseI TTAA 2 cut(s) 161, 929
MslI CAYNNNNRTG 3 cut(s) 246, 551, 729
MspI CCGG 2 cut(s) 575, 640
MspR9I CCNGG 1 cut(s) 575
MunI CAATTG 1 cut(s) 693
MwoI GCNNNNNNNGC 9 cut(s) 102, 193, 280, 334, 403, 473, 517, 845, 1051
NciI CCSGG 1 cut(s) 575
NdeI CATATG 2 cut(s) 785, 1229
NdeII GATC 2 cut(s) 67, 790
NlaIII CATG 5 cut(s) 245, 251, 552, 769, 925
NlaIV GGNNCC 6 cut(s) 144, 320, 388, 402, 418, 1020
NspI RCATGY 1 cut(s) 552
OliI CACNNNNGTG 1 cut(s) 551
PaeR7I CTCGAG 2 cut(s) 427, 865
PciI ACATGT 1 cut(s) 548
PciSI GCTCTTC 1 cut(s) 1069
PfeI GAWTC 2 cut(s) 1089, 1247
PkrI GCNGC 7 cut(s) 95, 98, 273, 314, 469, 669, 1155
PleI GAGTC 2 cut(s) 452, 957
PpsI GAGTC 2 cut(s) 452, 957
PpuMI RGGWCCY 1 cut(s) 400
PscI ACATGT 1 cut(s) 548
Psp124BI GAGCTC 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 400
PspN4I GGNNCC 6 cut(s) 144, 320, 388, 402, 418, 1020
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 6 cut(s) 318, 319, 387, 400, 417, 637
PspPPI RGGWCCY 1 cut(s) 400
PspXI VCTCGAGB 1 cut(s) 427
PstI CTGCAG 2 cut(s) 317, 1155
RsaI GTAC 2 cut(s) 595, 948
RsaNI GTAC 2 cut(s) 594, 947
RseI CAYNNNNRTG 3 cut(s) 246, 551, 729
SacI GAGCTC 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 1069
SaqAI TTAA 2 cut(s) 161, 929
SatI GCNGC 7 cut(s) 94, 97, 272, 313, 468, 668, 1154
Sau3AI GATC 2 cut(s) 67, 790
Sau96I GGNCC 6 cut(s) 318, 319, 387, 400, 417, 637
SchI GAGTC 2 cut(s) 452, 957
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 3 cut(s) 213, 322, 434
SfaNI GCATC 3 cut(s) 120, 258, 533
SfcI CTRYAG 3 cut(s) 35, 313, 1151
Sfr274I CTCGAG 2 cut(s) 427, 865
SinI GGWCC 4 cut(s) 387, 400, 417, 637
SlaI CTCGAG 2 cut(s) 427, 865
SmiMI CAYNNNNRTG 3 cut(s) 246, 551, 729
SmlI CTYRAG 4 cut(s) 427, 814, 865, 1012
SmoI CTYRAG 4 cut(s) 427, 814, 865, 1012
Sse9I AATT 8 cut(s) 30, 136, 229, 693, 808, 1035, 1108, 1146
SsiI CCGC 5 cut(s) 94, 404, 667, 670, 1079
SspMI CTAG 3 cut(s) 647, 1055, 1270
SstI GAGCTC 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 573
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 2 cut(s) 720, 1267
TaiI ACGT 2 cut(s) 168, 892
TaqI TCGA 3 cut(s) 66, 428, 866
TasI AATT 8 cut(s) 30, 136, 229, 693, 808, 1035, 1108, 1146
TauI GCSGC 2 cut(s) 96, 670
TfiI GAWTC 2 cut(s) 1089, 1247
Tru1I TTAA 2 cut(s) 161, 929
Tru9I TTAA 2 cut(s) 161, 929
TscAI CASTG 4 cut(s) 313, 498, 1128, 1270
TseI GCWGC 5 cut(s) 96, 271, 312, 467, 1153
TspDTI ATGAA 4 cut(s) 109, 258, 633, 989
TspGWI ACGGA 1 cut(s) 1149
TspRI CASTG 4 cut(s) 313, 498, 1128, 1270
VpaK11BI GGWCC 4 cut(s) 387, 400, 417, 637
XagI CCTNNNNNAGG 1 cut(s) 1206
XapI RAATTY 3 cut(s) 229, 1035, 1108
XceI RCATGY 1 cut(s) 552
XhoI CTCGAG 2 cut(s) 427, 865
XmiI GTMKAC 1 cut(s) 488
XspI CTAG 3 cut(s) 647, 1055, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.