MD09G1228600.v1.1

Belongs to the TUB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007280
Physical Location & Seq
Reverse (-)
28243733 .. 28246346
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1228600.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGATGGATGGTTGCAAGAAAACCACATTAATTCCACGGCAATCCTCATACAACTCTCTCTATGTCAATCCCCTCACAGACCAGAAACACAGCCGCAGCTACAGCGAAGGCATCGCCTTCACCTCCAAGAACGACAACTCGAACCCGAACCAATCACCCTTAAACGACAACAAGGAGAACGCAATGCCCAACAGGCCACAGCAGGCCGTGCCCGGCAGCGACAAGGAGAATTTGGTCGCACAAGAAAACGGGTCTTCCTCTTTTGCGATGCAGCCATTGAAGCCGAGGAATTTGAAGTCTCTGTCCACTGGCTGCAGGGCCCTCAAGCCCTCATCTCTTCAGTTCTGTATGCAGGCGAATGAGCCTGAGAAGGCACTCTTTGGTGGACCCATATCCACAATGTGGGACCCACCCAGCAAAGGGTCCGACAACTCGAGCTCTTTGAAGATCTGGGACTACTCCGATTCTGAGGCTGCCCCTGCCTCATCTTGGTCTACACTGCCTAACAAGTCATTGCTTTGCAGGCCATTGCCAATGGATGTCGGAAGGTGCACATGTGTTATAGTGAAGGAAGCAACCCCAGAAGGATTAGATGTGGGCACTCTCTATTCTCTTTATACCAATGAAGGCAAGGGAAGGCAGGACCGGAAACTAGCTATTGCTCACCATACGCGGCGGAACGGGAAATCTGAGTTAACTATTGCTCAGAATGTGAAGGGAATAATGTCTCATTCAGATGATAGTTTTATTGGGATTGTAAACGCTAACCTCATGGGTTCAAAATACCACATATGGGATCAGGGTGGTCGTCCCAATTCCTTGAGCAAAGAGGCTAAGTCACTTGCTGTTGTAGCGTTCACGCCAACAATCTCGAGCTGGACAGGAAGTTACAGACGTATGAGGGCATATATACCCAAGCACCAATCCATGCAGTTAAAAAACACAACGCAGGTACAACACATTAAGGGACTGCCAAAGGACTGGGAAGGGAAGCTTGACAAAGTCCATCAGCTATTCTCACGAGTTCCCCATTACAATAAAATTTCAAAGCAATATGAGTTAGACTTTAGAGATAGGGGAAGAGCGGGTCTTAGAATCCGAAGTTCAGTCAAGAATTTCCAACTGACACTGGAGGAAAACGGAAAGCAGACAATTCTGCAGCTCGGAAGGGTCGGGAAATTGAAGTATGTGATGGATTACAGATATCCTTTGACAGGCTACCAAGCGTTTTGCATATGTTTGGCTTCTATTGATTCAAAGCTTTGCTGCACTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tub

Protein Analysis

426

Amino Acids

47.41

Weight (kDa)

9.48

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 173 - 419 6e-48 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 940
AccB7I CCANNNNNTGG 1 cut(s) 402
AccBSI CCGCTC 1 cut(s) 1085
AccI GTMKAC 1 cut(s) 494
AccII CGCG 1 cut(s) 673
AciI CCGC 4 cut(s) 94, 673, 676, 1085
AclWI GGATC 1 cut(s) 804
AcsI RAATTY 4 cut(s) 229, 289, 1041, 1114
AcuI CTGAAG 1 cut(s) 323
AdeI CACNNNGTG 1 cut(s) 402
AfaI GTAC 1 cut(s) 954
AfiI CCNNNNNNNGG 6 cut(s) 402, 419, 420, 489, 793, 1214
AflIII ACRYGT 1 cut(s) 554
AgsI TTSAA 7 cut(s) 280, 295, 445, 780, 1047, 1183, 1257
AluBI AGCT 8 cut(s) 99, 438, 656, 876, 994, 1012, 1162, 1261
AluI AGCT 8 cut(s) 99, 438, 656, 876, 994, 1012, 1162, 1261
Alw21I GWGCWC 2 cut(s) 440, 554
Alw26I GTCTC 2 cut(s) 303, 732
Alw44I GTGCAC 1 cut(s) 550
AlwI GGATC 1 cut(s) 804
Ama87I CYCGRG 2 cut(s) 433, 871
AoxI GGCC 4 cut(s) 194, 204, 318, 524
ApaI GGGCCC 1 cut(s) 322
ApaLI GTGCAC 1 cut(s) 550
ApeKI GCWGC 7 cut(s) 96, 216, 271, 312, 473, 1159, 1266
ApoI RAATTY 4 cut(s) 229, 289, 1041, 1114
AseI ATTAAT 1 cut(s) 29
AspS9I GGNCC 6 cut(s) 318, 319, 386, 406, 423, 643
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 3 cut(s) 112, 147, 656
AvaI CYCGRG 2 cut(s) 433, 871
AvaII GGWCC 4 cut(s) 386, 406, 423, 643
BaeGI GKGCMC 4 cut(s) 213, 322, 554, 602
BanII GRGCYC 2 cut(s) 322, 440
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 246
Bbv12I GWGCWC 2 cut(s) 440, 554
BbvI GCAGC 7 cut(s) 108, 228, 283, 299, 460, 1171, 1253
BccI CCATC 3 cut(s) 2, 1014, 1186
BceAI ACGGC 2 cut(s) 53, 191
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 303, 732
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 3 cut(s) 100, 313, 1157
BfuAI ACCTGC 1 cut(s) 940
BglI GCCNNNNNGGC 1 cut(s) 193
BglII AGATCT 1 cut(s) 447
BisI GCNGC 9 cut(s) 94, 97, 217, 272, 313, 474, 674, 1160, 1267
BlsI GCNGC 9 cut(s) 95, 98, 218, 273, 314, 475, 675, 1161, 1268
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 4 cut(s) 386, 406, 423, 643
BmeT110I CYCGRG 2 cut(s) 433, 871
BmgT120I GGNCC 6 cut(s) 318, 319, 386, 406, 423, 643
BmiI GGNNCC 5 cut(s) 320, 388, 407, 408, 424
BmrFI CCNGG 1 cut(s) 213
BmrI ACTGGG 1 cut(s) 991
BmsI GCATC 2 cut(s) 120, 258
BmuI ACTGGG 1 cut(s) 991
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 1151
BpuEI CTTGAG 2 cut(s) 308, 841
BpuMI CCSGG 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 35, 284
BsaWI WCCGGW 1 cut(s) 645
Bsc4I CCNNNNNNNGG 6 cut(s) 402, 419, 420, 489, 793, 1214
Bse1I ACTGG 3 cut(s) 313, 986, 1134
Bse3DI GCAATG 3 cut(s) 189, 512, 527
BseDI CCNNGG 2 cut(s) 35, 284
BseGI GGATG 2 cut(s) 13, 544
BseLI CCNNNNNNNGG 6 cut(s) 402, 419, 420, 489, 793, 1214
BseMI GCAATG 3 cut(s) 189, 512, 527
BseMII CTCAG 4 cut(s) 357, 459, 681, 719
BseNI ACTGG 3 cut(s) 313, 986, 1134
BseSI GKGCMC 4 cut(s) 213, 322, 554, 602
BseXI GCAGC 7 cut(s) 108, 228, 283, 299, 460, 1171, 1253
BseYI CCCAGC 1 cut(s) 413
BsgI GTGCAG 1 cut(s) 1252
Bsh1236I CGCG 1 cut(s) 673
BshFI GGCC 4 cut(s) 196, 206, 320, 526
BsiHKAI GWGCWC 2 cut(s) 440, 554
BsiHKCI CYCGRG 2 cut(s) 433, 871
BsiSI CCGG 2 cut(s) 213, 646
BslFI GGGAC 4 cut(s) 419, 467, 795, 981
BslI CCNNNNNNNGG 6 cut(s) 402, 419, 420, 489, 793, 1214
BsmAI GTCTC 2 cut(s) 303, 732
BsmFI GGGAC 4 cut(s) 419, 467, 795, 981
BsnI GGCC 4 cut(s) 196, 206, 320, 526
BsoBI CYCGRG 2 cut(s) 433, 871
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 5 cut(s) 213, 322, 440, 554, 602
Bsp143I GATC 2 cut(s) 447, 796
BspACI CCGC 4 cut(s) 94, 673, 676, 1085
BspANI GGCC 4 cut(s) 196, 206, 320, 526
BspCNI CTCAG 4 cut(s) 358, 460, 682, 718
BspFNI CGCG 1 cut(s) 673
BspLI GGNNCC 5 cut(s) 320, 388, 407, 408, 424
BspMAI CTGCAG 2 cut(s) 317, 1161
BspMI ACCTGC 1 cut(s) 940
BspPI GGATC 1 cut(s) 804
BspQI GCTCTTC 1 cut(s) 1075
BsrBI CCGCTC 1 cut(s) 1085
BsrDI GCAATG 3 cut(s) 189, 512, 527
BsrI ACTGG 3 cut(s) 313, 986, 1134
BssECI CCNNGG 2 cut(s) 35, 284
BssMI GATC 2 cut(s) 447, 796
BssSI CACGAG 1 cut(s) 1020
Bst2BI CACGAG 1 cut(s) 1020
Bst4CI ACNGT 1 cut(s) 1273
Bst6I CTCTTC 2 cut(s) 342, 1075
BstAPI GCANNNNNTGC 1 cut(s) 208
BstC8I GCNNGC 3 cut(s) 204, 354, 524
BstDEI CTNAG 6 cut(s) 366, 468, 690, 705, 834, 1091
BstDSI CCRYGG 1 cut(s) 35
BstENI CCTNNNNNAGG 1 cut(s) 1212
BstF5I GGATG 2 cut(s) 13, 544
BstFNI CGCG 1 cut(s) 673
BstKTI GATC 2 cut(s) 450, 799
BstMAI GTCTC 2 cut(s) 303, 732
BstMBI GATC 2 cut(s) 447, 796
BstMWI GCNNNNNNNGC 7 cut(s) 102, 193, 208, 280, 479, 523, 851
BstNSI RCATGY 1 cut(s) 558
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 3 cut(s) 100, 313, 1157
BstSLI GKGCMC 4 cut(s) 213, 322, 554, 602
BstUI CGCG 1 cut(s) 673
BstV1I GCAGC 7 cut(s) 108, 228, 283, 299, 460, 1171, 1253
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 447
BstXI CCANNNNNNTGG 1 cut(s) 981
BstYI RGATCY 1 cut(s) 447
BsuRI GGCC 4 cut(s) 196, 206, 320, 526
BtgI CCRYGG 1 cut(s) 35
BtgZI GCGATG 2 cut(s) 97, 281
BtsCI GGATG 2 cut(s) 13, 544
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 4 cut(s) 306, 497, 1127, 1269
BveI ACCTGC 1 cut(s) 940
Cac8I GCNNGC 3 cut(s) 204, 354, 524
Cfr13I GGNCC 6 cut(s) 318, 319, 386, 406, 423, 643
Csp6I GTAC 1 cut(s) 953
CviAII CATG 3 cut(s) 555, 772, 928
CviQI GTAC 1 cut(s) 953
DdeI CTNAG 6 cut(s) 366, 468, 690, 705, 834, 1091
DpnI GATC 2 cut(s) 449, 798
DpnII GATC 2 cut(s) 447, 796
DraIII CACNNNGTG 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 342, 1075
EarI CTCTTC 2 cut(s) 342, 1075
EciI GGCGGA 1 cut(s) 691
Ecl136II GAGCTC 1 cut(s) 438
Eco24I GRGCYC 2 cut(s) 322, 440
Eco32I GATATC 1 cut(s) 1205
Eco47I GGWCC 4 cut(s) 386, 406, 423, 643
Eco53kI GAGCTC 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 323
Eco88I CYCGRG 2 cut(s) 433, 871
EcoICRI GAGCTC 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 1212
EcoO109I RGGNCCY 3 cut(s) 318, 319, 406
EcoRV GATATC 1 cut(s) 1205
EcoT38I GRGCYC 2 cut(s) 322, 440
FaeI CATG 3 cut(s) 558, 775, 931
FalI AAGNNNNNCTT 4 cut(s) 362, 394, 978, 1010
FaqI GGGAC 4 cut(s) 419, 467, 795, 981
FatI CATG 3 cut(s) 554, 771, 927
FauI CCCGC 1 cut(s) 1078
FauNDI CATATG 2 cut(s) 791, 1235
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 9 cut(s) 94, 97, 217, 272, 313, 474, 674, 1160, 1267
FokI GGATG 2 cut(s) 20, 551
FriOI GRGCYC 2 cut(s) 322, 440
Fsp4HI GCNGC 9 cut(s) 94, 97, 217, 272, 313, 474, 674, 1160, 1267
FspBI CTAG 1 cut(s) 653
GluI GCNGC 9 cut(s) 94, 97, 217, 272, 313, 474, 674, 1160, 1267
GsaI CCCAGC 1 cut(s) 417
GsuI CTGGAG 1 cut(s) 1151
HaeIII GGCC 4 cut(s) 196, 206, 320, 526
HapII CCGG 2 cut(s) 213, 646
Hin1II CATG 3 cut(s) 558, 775, 931
HincII GTYRAC 1 cut(s) 696
HindII GTYRAC 1 cut(s) 696
HindIII AAGCTT 2 cut(s) 992, 1259
HinfI GANTC 3 cut(s) 464, 1095, 1253
HpaI GTTAAC 1 cut(s) 696
HpaII CCGG 2 cut(s) 213, 646
HphI GGTGA 3 cut(s) 112, 147, 656
Hpy166II GTNNAC 7 cut(s) 306, 386, 495, 552, 696, 760, 858
Hpy188I TCNGA 9 cut(s) 427, 463, 469, 545, 691, 708, 736, 1100, 1166
Hpy188III TCNNGA 4 cut(s) 871, 1020, 1111, 1174
Hpy8I GTNNAC 7 cut(s) 306, 386, 495, 552, 696, 760, 858
HpyCH4III ACNGT 1 cut(s) 1273
HpyCH4IV ACGT 1 cut(s) 895
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 193, 208, 280, 479, 523, 851
HpyF3I CTNAG 6 cut(s) 366, 468, 690, 705, 834, 1091
HpySE526I ACGT 1 cut(s) 895
Hsp92II CATG 3 cut(s) 558, 775, 931
KflI GGGWCCC 1 cut(s) 406
KspAI GTTAAC 1 cut(s) 696
Kzo9I GATC 2 cut(s) 447, 796
LguI GCTCTTC 1 cut(s) 1075
Lsp1109I GCAGC 7 cut(s) 108, 228, 283, 299, 460, 1171, 1253
LweI GCATC 2 cut(s) 120, 258
MaeI CTAG 1 cut(s) 653
MaeII ACGT 1 cut(s) 895
MaeIII GTNAC 2 cut(s) 837, 887
MalI GATC 2 cut(s) 449, 798
MbiI CCGCTC 1 cut(s) 1085
MboI GATC 2 cut(s) 447, 796
MboII GAAGA 4 cut(s) 246, 329, 457, 1092
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 5 cut(s) 213, 322, 440, 554, 602
MluCI AATT 8 cut(s) 30, 229, 289, 814, 1041, 1114, 1152, 1178
MmeI TCCRAC 3 cut(s) 450, 523, 1144
MseI TTAA 5 cut(s) 29, 161, 695, 935, 963
MslI CAYNNNNRTG 1 cut(s) 735
MspI CCGG 2 cut(s) 213, 646
MspR9I CCNGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 673
MwoI GCNNNNNNNGC 7 cut(s) 102, 193, 208, 280, 479, 523, 851
NciI CCSGG 1 cut(s) 213
NdeI CATATG 2 cut(s) 791, 1235
NdeII GATC 2 cut(s) 447, 796
NlaIII CATG 3 cut(s) 558, 775, 931
NlaIV GGNNCC 5 cut(s) 320, 388, 407, 408, 424
NmeAIII GCCGAG 1 cut(s) 309
NmuCI GTSAC 1 cut(s) 837
NspI RCATGY 1 cut(s) 558
PaeR7I CTCGAG 2 cut(s) 433, 871
PciI ACATGT 1 cut(s) 554
PciSI GCTCTTC 1 cut(s) 1075
PfeI GAWTC 3 cut(s) 464, 1095, 1253
PflFI GACNNNGTC 1 cut(s) 1001
PflMI CCANNNNNTGG 1 cut(s) 402
PkrI GCNGC 9 cut(s) 95, 98, 218, 273, 314, 475, 675, 1161, 1268
PpuMI RGGWCCY 1 cut(s) 406
PscI ACATGT 1 cut(s) 554
PshBI ATTAAT 1 cut(s) 29
Psp124BI GAGCTC 1 cut(s) 440
Psp5II RGGWCCY 1 cut(s) 406
PspFI CCCAGC 1 cut(s) 413
PspN4I GGNNCC 5 cut(s) 320, 388, 407, 408, 424
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 6 cut(s) 318, 319, 386, 406, 423, 643
PspPPI RGGWCCY 1 cut(s) 406
PspXI VCTCGAGB 1 cut(s) 433
PstI CTGCAG 2 cut(s) 317, 1161
PsuI RGATCY 1 cut(s) 447
PsyI GACNNNGTC 1 cut(s) 1001
RsaI GTAC 1 cut(s) 954
RsaNI GTAC 1 cut(s) 953
RseI CAYNNNNRTG 1 cut(s) 735
SacI GAGCTC 1 cut(s) 440
SapI GCTCTTC 1 cut(s) 1075
SaqAI TTAA 5 cut(s) 29, 161, 695, 935, 963
SatI GCNGC 9 cut(s) 94, 97, 217, 272, 313, 474, 674, 1160, 1267
Sau3AI GATC 2 cut(s) 447, 796
Sau96I GGNCC 6 cut(s) 318, 319, 386, 406, 423, 643
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 5 cut(s) 213, 322, 440, 554, 602
SfaNI GCATC 2 cut(s) 120, 258
SfcI CTRYAG 3 cut(s) 100, 313, 1157
Sfr274I CTCGAG 2 cut(s) 433, 871
SinI GGWCC 4 cut(s) 386, 406, 423, 643
SlaI CTCGAG 2 cut(s) 433, 871
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 4 cut(s) 323, 433, 820, 871
SmoI CTYRAG 4 cut(s) 323, 433, 820, 871
Sse9I AATT 8 cut(s) 30, 229, 289, 814, 1041, 1114, 1152, 1178
SsiI CCGC 4 cut(s) 94, 673, 676, 1085
SspMI CTAG 1 cut(s) 653
SstI GAGCTC 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 1273
TaiI ACGT 1 cut(s) 898
TaqI TCGA 3 cut(s) 140, 434, 872
TasI AATT 8 cut(s) 30, 229, 289, 814, 1041, 1114, 1152, 1178
TauI GCSGC 2 cut(s) 96, 676
TfiI GAWTC 3 cut(s) 464, 1095, 1253
Tru1I TTAA 5 cut(s) 29, 161, 695, 935, 963
Tru9I TTAA 5 cut(s) 29, 161, 695, 935, 963
TscAI CASTG 4 cut(s) 313, 504, 1134, 1276
TseFI GTSAC 1 cut(s) 837
TseI GCWGC 7 cut(s) 96, 216, 271, 312, 473, 1159, 1266
Tsp45I GTSAC 1 cut(s) 837
TspDTI ATGAA 1 cut(s) 639
TspGWI ACGGA 1 cut(s) 1155
TspRI CASTG 4 cut(s) 313, 504, 1134, 1276
Tth111I GACNNNGTC 1 cut(s) 1001
Van91I CCANNNNNTGG 1 cut(s) 402
VneI GTGCAC 1 cut(s) 550
VpaK11BI GGWCC 4 cut(s) 386, 406, 423, 643
VspI ATTAAT 1 cut(s) 29
XagI CCTNNNNNAGG 1 cut(s) 1212
XapI RAATTY 4 cut(s) 229, 289, 1041, 1114
XceI RCATGY 1 cut(s) 558
XhoI CTCGAG 2 cut(s) 433, 871
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.