FvH4_6g30380

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012640
Physical Location & Seq
Forward (+)
23444810 .. 23448835
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30380.t2

Sequence Viewer

Length: 1479 bp
ATGCCCAAAGTCAGACGATCATCTTCCTTGTCTCTTGGTCGATCTAGGGCATCTCCTTATCCTTGCACCTCTGAGGATGCTGAGCATTACAAGTTGAATAGTCCGTTGGAAAGCACAGAGAATGCTAAAGAATGGGAAGATACTAGGTGCCCCATCTGCATGGAACACCCACATAATGCTGTTCTTATAAAATGTTCCTCTCATGAGAAGGGCTGCAGGCCGTTCATGTGCAATACAAGCTACCGGCACTCAAATTGTCTTGACCAGTTTTGTAAGTCTTCTGTTCCCGACACCACACCAATGACATTGCAAGAACTTCCTCTGGCGCACACAGCCGCTCACAGTTCTAGGGAAGAAGAATCGCTGTCTGGGCAAACCAGCCCTTGTGGGAGTCAGTTGCAACTACATCCAGCTTGCCCTTTTTGTCGGGGAAAGATCTATGGGTACATTGTTGTACGGGCTGCTCGTCACTTCATGAACTGCAAAGCGAGGAGCTGCTCTTCTGAGACATGTGACTTTACTGGTACTTATTCAGAGCTGAGGAAGCATGCGAGGTCTGAACACCCTTATGTCCGGCCGGCAGAGGTGGATCCAAATCGACATCGTGATTGGATGAGATTGGAGCGTCAAAGGGACTGGGAAGACGTACTTAGCATAGTCCGAGTAGGATCTGAAGATGATATTATTCCGGATTCATCAACTGATAGTGGCTGGATGACTTCCCTTTTGAATGCTATGTTTCACTCGTTTGAGATCATGATTGTTTCCCATTTGATGGAGAGTTCAAGTGACATAGAACAAACACATAGTAGAAGATCAGGGAGAACGACTAGGGTTCACCAAGACAATAACACAGTCTCTCCCACGAGAAGGAACAACACTGTATCAGAGAGCAGATCCCATCGCAGTGGTCTACTTTATCGAGCAACAAGTAATAATGTTGAGACAAGTCGTGCCCCCAGACGGAGCAACAATGTCTTGTCAGGGGAGATTGGCCATGCCCCCCCTAGACGGAGCAACAATTTCATGTCAGGGGAGATTGGCCATGCCCCCCCTAGACGGAGCAACAATTTAGTGTTAGGGGAGGCTGGCCATGCCCCCTCTAGAAGGAGCAACAATTCATTGTCAGGGGAGACTGCCCATGCCCCCCCTAGACGCAGCAACAACTCATTGGTGGAGGCTAGTGCCCCTAGAAGGGGTAACTCTTTCTCTGTGGTGGGTACTAACCATGTTCTTAGAAGGGGTAACAACACTGTATTGGGCAACAACTCCGTGCCAGAGGAGAGTAGGCGTAGACGGGGAAGCTATTCTGTATCCGAGGAGAGTAATCATGTCCACGGATGGGGCAACAACTCCTTATCAGAAGAAGGTTGGCAACCTCCTAGATGGGGCAATACTTCTGTACAAGAAAGCACACCCCGTCCTGGGAGACTGAGATTTAGACATCAAACATGGCCAACAAACAATAACAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

493

Amino Acids

55.42

Weight (kDa)

9.34

Isoelectric Point (pI)

73.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 46 - 218 1.5e-66 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 775
AccBSI CCGCTC 1 cut(s) 338
AccI GTMKAC 2 cut(s) 913, 1294
AccIII TCCGGA 1 cut(s) 688
AciI CCGC 1 cut(s) 336
AclWI GGATC 4 cut(s) 584, 597, 676, 891
AcoI YGGCCR 5 cut(s) 575, 994, 1042, 1090, 1454
AcuI CTGAAG 1 cut(s) 693
AfaI GTAC 6 cut(s) 446, 456, 526, 648, 1222, 1404
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 3 cut(s) 97, 730, 786
AjnI CCWGG 1 cut(s) 1423
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 5 cut(s) 240, 413, 495, 538, 1305
AluI AGCT 5 cut(s) 240, 413, 495, 538, 1305
Alw26I GTCTC 6 cut(s) 36, 500, 862, 938, 1127, 1423
AlwI GGATC 4 cut(s) 584, 597, 676, 891
Aor13HI TCCGGA 1 cut(s) 688
AoxI GGCC 6 cut(s) 218, 575, 994, 1042, 1090, 1454
ApeKI GCWGC 4 cut(s) 213, 461, 495, 1158
ArsI GACNNNNNNTTYG 2 cut(s) 709, 741
Asp700I GAANNNNTTC 1 cut(s) 1306
AspLEI GCGC 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 830
BaeGI GKGCMC 3 cut(s) 152, 958, 1189
BalI TGGCCA 4 cut(s) 996, 1044, 1092, 1456
BamHI GGATCC 1 cut(s) 589
BanI GGYRCC 1 cut(s) 147
BauI CACGAG 1 cut(s) 865
BbsI GAAGAC 2 cut(s) 270, 648
BbvCI CCTCAGC 1 cut(s) 539
BbvI GCAGC 4 cut(s) 200, 448, 482, 1170
BccI CCATC 5 cut(s) 161, 769, 909, 1335, 1380
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 1 cut(s) 1425
BciVI GTATCC 1 cut(s) 1324
BcoDI GTCTC 6 cut(s) 36, 500, 862, 938, 1127, 1423
BfmI CTRYAG 1 cut(s) 214
BfuI GTATCC 1 cut(s) 1324
BglII AGATCT 1 cut(s) 435
BisI GCNGC 5 cut(s) 214, 336, 462, 496, 1159
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 5 cut(s) 215, 337, 463, 497, 1160
Bme1390I CCNGG 1 cut(s) 1425
BmiI GGNNCC 2 cut(s) 149, 591
BmrFI CCNGG 1 cut(s) 1425
BmrI ACTGGG 1 cut(s) 646
BmsI GCATC 2 cut(s) 59, 67
BmuI ACTGGG 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 270, 648
Bpu10I CCTNAGC 1 cut(s) 539
Bpu1102I GCTNAGC 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 594
BsaJI CCNNGG 3 cut(s) 1317, 1336, 1424
BsaWI WCCGGW 1 cut(s) 688
BsaXI ACNNNNNCTCC 2 cut(s) 844, 874
Bse118I RCCGGY 2 cut(s) 243, 577
Bse1I ACTGG 3 cut(s) 265, 526, 641
Bse3DI GCAATG 1 cut(s) 305
Bse8I GATNNNNATC 1 cut(s) 594
BseAI TCCGGA 1 cut(s) 688
BseBI CCWGG 1 cut(s) 1425
BseDI CCNNGG 3 cut(s) 1317, 1336, 1424
BseGI GGATG 5 cut(s) 82, 406, 618, 720, 1346
BseJI GATNNNNATC 1 cut(s) 594
BseMI GCAATG 1 cut(s) 305
BseMII CTCAG 5 cut(s) 63, 72, 495, 530, 1424
BseNI ACTGG 3 cut(s) 265, 526, 641
BseRI GAGGAG 3 cut(s) 505, 1295, 1334
BseSI GKGCMC 3 cut(s) 152, 958, 1189
BseX3I CGGCCG 1 cut(s) 575
BseXI GCAGC 4 cut(s) 200, 448, 482, 1170
Bsh1285I CGRYCG 1 cut(s) 578
BshFI GGCC 6 cut(s) 220, 577, 996, 1044, 1092, 1456
BshNI GGYRCC 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 578
BsiSI CCGG 4 cut(s) 244, 574, 578, 689
BslFI GGGAC 1 cut(s) 647
BsmAI GTCTC 6 cut(s) 36, 500, 862, 938, 1127, 1423
BsmFI GGGAC 1 cut(s) 647
BsmI GAATGC 2 cut(s) 127, 736
BsnI GGCC 6 cut(s) 220, 577, 996, 1044, 1092, 1456
Bsp1286I GDGCHC 3 cut(s) 152, 958, 1189
Bsp13I TCCGGA 1 cut(s) 688
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
Bsp1720I GCTNAGC 1 cut(s) 81
BspACI CCGC 1 cut(s) 336
BspANI GGCC 6 cut(s) 220, 577, 996, 1044, 1092, 1456
BspCNI CTCAG 5 cut(s) 64, 73, 496, 531, 1425
BspEI TCCGGA 1 cut(s) 688
BspHI TCATGA 3 cut(s) 202, 474, 756
BspLI GGNNCC 2 cut(s) 149, 591
BspMAI CTGCAG 1 cut(s) 218
BspPI GGATC 4 cut(s) 584, 597, 676, 891
BspQI GCTCTTC 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 147
BsrBI CCGCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 305
BsrFI RCCGGY 2 cut(s) 243, 577
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 3 cut(s) 265, 526, 641
BssAI RCCGGY 2 cut(s) 243, 577
BssECI CCNNGG 3 cut(s) 1317, 1336, 1424
BssMI GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
BssSI CACGAG 1 cut(s) 865
Bst2BI CACGAG 1 cut(s) 865
Bst2UI CCWGG 1 cut(s) 1425
Bst4CI ACNGT 4 cut(s) 344, 856, 883, 1255
Bst6I CTCTTC 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 1402
BstC8I GCNNGC 5 cut(s) 218, 415, 549, 579, 1090
BstDEI CTNAG 7 cut(s) 72, 81, 504, 539, 650, 1235, 1433
BstDSI CCRYGG 1 cut(s) 1336
BstENI CCTNNNNNAGG 1 cut(s) 1105
BstF5I GGATG 5 cut(s) 82, 406, 618, 720, 1346
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 8 cut(s) 20, 44, 438, 592, 671, 756, 818, 899
BstMAI GTCTC 6 cut(s) 36, 500, 862, 938, 1127, 1423
BstMBI GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
BstMCI CGRYCG 1 cut(s) 578
BstMWI GCNNNNNNNGC 6 cut(s) 156, 237, 332, 370, 544, 1094
BstNI CCWGG 1 cut(s) 1425
BstNSI RCATGY 2 cut(s) 513, 551
BstSCI CCNGG 1 cut(s) 1423
BstSFI CTRYAG 1 cut(s) 214
BstSLI GKGCMC 3 cut(s) 152, 958, 1189
BstV1I GCAGC 4 cut(s) 200, 448, 482, 1170
BstV2I GAAGAC 2 cut(s) 270, 648
BstX2I RGATCY 4 cut(s) 435, 589, 668, 896
BstXI CCANNNNNNTGG 2 cut(s) 160, 908
BstYI RGATCY 4 cut(s) 435, 589, 668, 896
BstZI CGGCCG 1 cut(s) 575
BsuI GTATCC 1 cut(s) 1324
BsuRI GGCC 6 cut(s) 220, 577, 996, 1044, 1092, 1456
BtgI CCRYGG 1 cut(s) 1336
BtgZI GCGATG 1 cut(s) 887
BtsCI GGATG 5 cut(s) 82, 406, 618, 720, 1346
BtsI GCAGTG 1 cut(s) 913
BtsIMutI CAGTG 3 cut(s) 879, 913, 1251
Cac8I GCNNGC 5 cut(s) 218, 415, 549, 579, 1090
CciI TCATGA 3 cut(s) 202, 474, 756
CfoI GCGC 1 cut(s) 328
Cfr10I RCCGGY 2 cut(s) 243, 577
CseI GACGC 2 cut(s) 614, 1164
Csp6I GTAC 6 cut(s) 445, 455, 525, 647, 1221, 1403
CviQI GTAC 6 cut(s) 445, 455, 525, 647, 1221, 1403
DdeI CTNAG 7 cut(s) 72, 81, 504, 539, 650, 1235, 1433
DpnI GATC 8 cut(s) 19, 43, 437, 591, 670, 755, 817, 898
DpnII GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
EaeI YGGCCR 5 cut(s) 575, 994, 1042, 1090, 1454
EagI CGGCCG 1 cut(s) 575
Eam1104I CTCTTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 505
EclXI CGGCCG 1 cut(s) 575
Eco52I CGGCCG 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 693
EcoNI CCTNNNNNAGG 1 cut(s) 1105
EcoRII CCWGG 1 cut(s) 1423
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FaqI GGGAC 1 cut(s) 647
FblI GTMKAC 2 cut(s) 913, 1294
Fnu4HI GCNGC 5 cut(s) 214, 336, 462, 496, 1159
FokI GGATG 5 cut(s) 89, 393, 625, 727, 1353
Fsp4HI GCNGC 5 cut(s) 214, 336, 462, 496, 1159
GlaI GCGC 1 cut(s) 327
GluI GCNGC 5 cut(s) 214, 336, 462, 496, 1159
HaeIII GGCC 6 cut(s) 220, 577, 996, 1044, 1092, 1456
HapII CCGG 4 cut(s) 244, 574, 578, 689
HgaI GACGC 2 cut(s) 614, 1164
HhaI GCGC 1 cut(s) 328
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HinfI GANTC 3 cut(s) 359, 391, 692
HpaII CCGG 4 cut(s) 244, 574, 578, 689
HphI GGTGA 1 cut(s) 830
Hpy166II GTNNAC 4 cut(s) 838, 914, 1295, 1336
Hpy188III TCNNGA 8 cut(s) 203, 260, 287, 475, 605, 689, 757, 1104
Hpy8I GTNNAC 4 cut(s) 838, 914, 1295, 1336
HpyAV CCTTC 7 cut(s) 202, 864, 1101, 1188, 1233, 1361, 1467
HpyCH4III ACNGT 4 cut(s) 344, 856, 883, 1255
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 7 cut(s) 66, 159, 216, 231, 310, 400, 483
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 237, 332, 370, 544, 1094
HpyF3I CTNAG 7 cut(s) 72, 81, 504, 539, 650, 1235, 1433
HpySE526I ACGT 1 cut(s) 645
HspAI GCGC 1 cut(s) 326
Kpn2I TCCGGA 1 cut(s) 688
KroI GCCGGC 1 cut(s) 577
KroNI GCCGGC 1 cut(s) 579
Kzo9I GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
LguI GCTCTTC 1 cut(s) 505
LmnI GCTCC 6 cut(s) 492, 622, 966, 1014, 1062, 1110
Lsp1109I GCAGC 4 cut(s) 200, 448, 482, 1170
LweI GCATC 2 cut(s) 59, 67
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 5 cut(s) 467, 512, 788, 1199, 1244
MalI GATC 8 cut(s) 19, 43, 437, 591, 670, 755, 817, 898
MbiI CCGCTC 1 cut(s) 338
MboI GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
MflI RGATCY 4 cut(s) 435, 589, 668, 896
MhlI GDGCHC 3 cut(s) 152, 958, 1189
MlsI TGGCCA 4 cut(s) 996, 1044, 1092, 1456
MluCI AATT 4 cut(s) 253, 1021, 1069, 1117
MluNI TGGCCA 4 cut(s) 996, 1044, 1092, 1456
MlyI GAGTC 1 cut(s) 400
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 4 cut(s) 996, 1044, 1092, 1456
MroI TCCGGA 1 cut(s) 688
MroNI GCCGGC 1 cut(s) 577
MroXI GAANNNNTTC 1 cut(s) 1306
MscI TGGCCA 4 cut(s) 996, 1044, 1092, 1456
MslI CAYNNNNRTG 4 cut(s) 158, 299, 567, 906
Msp20I TGGCCA 4 cut(s) 996, 1044, 1092, 1456
MspI CCGG 4 cut(s) 244, 574, 578, 689
MspR9I CCNGG 1 cut(s) 1425
Mva1269I GAATGC 2 cut(s) 127, 736
MvaI CCWGG 1 cut(s) 1425
MwoI GCNNNNNNNGC 6 cut(s) 156, 237, 332, 370, 544, 1094
NaeI GCCGGC 1 cut(s) 579
NdeII GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
NgoMIV GCCGGC 1 cut(s) 577
NlaIV GGNNCC 2 cut(s) 149, 591
NmuCI GTSAC 3 cut(s) 467, 512, 788
NspI RCATGY 2 cut(s) 513, 551
PaeI GCATGC 1 cut(s) 551
PagI TCATGA 3 cut(s) 202, 474, 756
PciI ACATGT 1 cut(s) 509
PciSI GCTCTTC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 463
PctI GAATGC 2 cut(s) 127, 736
PdiI GCCGGC 1 cut(s) 579
PdmI GAANNNNTTC 1 cut(s) 1306
PfeI GAWTC 2 cut(s) 359, 692
PflMI CCANNNNNTGG 1 cut(s) 775
PkrI GCNGC 5 cut(s) 215, 337, 463, 497, 1160
PleI GAGTC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 399
PscI ACATGT 1 cut(s) 509
PsiI TTATAA 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 1423
PspGI CCWGG 1 cut(s) 1423
PspN4I GGNNCC 2 cut(s) 149, 591
PstI CTGCAG 1 cut(s) 218
PsuI RGATCY 4 cut(s) 435, 589, 668, 896
RsaI GTAC 6 cut(s) 446, 456, 526, 648, 1222, 1404
RsaNI GTAC 6 cut(s) 445, 455, 525, 647, 1221, 1403
RseI CAYNNNNRTG 4 cut(s) 158, 299, 567, 906
SapI GCTCTTC 1 cut(s) 505
SatI GCNGC 5 cut(s) 214, 336, 462, 496, 1159
Sau3AI GATC 8 cut(s) 17, 41, 435, 589, 668, 753, 815, 896
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 1425
SduI GDGCHC 3 cut(s) 152, 958, 1189
SfaNI GCATC 2 cut(s) 59, 67
SfcI CTRYAG 1 cut(s) 214
SmiMI CAYNNNNRTG 4 cut(s) 158, 299, 567, 906
SphI GCATGC 1 cut(s) 551
Sse9I AATT 4 cut(s) 253, 1021, 1069, 1117
SsiI CCGC 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 1423
TaaI ACNGT 4 cut(s) 344, 856, 883, 1255
TaiI ACGT 1 cut(s) 648
TaqI TCGA 3 cut(s) 40, 598, 922
TasI AATT 4 cut(s) 253, 1021, 1069, 1117
TatI WGTACW 1 cut(s) 1402
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 2 cut(s) 359, 692
TscAI CASTG 3 cut(s) 886, 913, 1258
TseFI GTSAC 3 cut(s) 467, 512, 788
TseI GCWGC 4 cut(s) 213, 461, 495, 1158
Tsp45I GTSAC 3 cut(s) 467, 512, 788
TspDTI ATGAA 6 cut(s) 214, 463, 491, 684, 1015, 1110
TspGWI ACGGA 6 cut(s) 93, 979, 1027, 1075, 1261, 1353
TspRI CASTG 3 cut(s) 886, 913, 1258
Van91I CCANNNNNTGG 1 cut(s) 775
XagI CCTNNNNNAGG 1 cut(s) 1105
XbaI TCTAGA 1 cut(s) 1103
XceI RCATGY 2 cut(s) 513, 551
XmiI GTMKAC 2 cut(s) 913, 1294
XmnI GAANNNNTTC 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.