Rorug02G0332600

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011348
Physical Location & Seq
Forward (+)
41208927 .. 41211346
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0332600.1

Sequence Viewer

Length: 606 bp
ATGGAGACGAGTTTCATATGGTCAGTCTCCGCCTGTTTCTTTCTGGTCTCGTCCTTCTCCTTTGTTCGCTCATTCTCCAATCCATCTCTTCTTGGAGTTCATCCACTCGATGAAAAGTACTTTGCTACAGAGGTGATCAAGTGCAAGGACGACTCCAAGTCGTTCACCAGAGACCGTCTCAATGATAATTTCTGTGACTGTGTTGATGGGACTGACGAGCCCGGGACTTCTGCTTGTCCTGCAGGCAAATTTTATTGTCGGAATATTGGAAGTACACCACAGTTCATATTTTCTTCTCGAGTTAATGATCGTATTTGTGATTGCTGCGATGGAAGTGATGAGAATGATGGTAGTCTCAATTGTCCAAATACATGCATCATGGGCGGGAATGTTGAGTACAAAACTGACCACCGCTTCTCTAGAGTTCAGGATCGAGGGTATGTTGACAAAAAGCAAACCGAAAATAGGGTCAGTCTGGATGACTTCATTCAGAAGCTTAAAGGTTTGAAGATAATAATAATCGTACAATTAGTTATTGCTAGTGTTTTTGTGGTTTGCCGGATATTCCATCGTCGTGCCAGATCAAAACGAAGACGTCATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

201

Amino Acids

22.93

Weight (kDa)

8.6

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 27 - 137 3.3e-30 Glucosidase II beta subunit-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 598
AciI CCGC 3 cut(s) 30, 384, 412
AclWI GGATC 1 cut(s) 438
AcsI RAATTY 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 595
AfaI GTAC 4 cut(s) 119, 274, 398, 525
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 1 cut(s) 508
AhdI GACNNNNNGTC 1 cut(s) 157
AluBI AGCT 1 cut(s) 496
AluI AGCT 1 cut(s) 496
Alw26I GTCTC 5 cut(s) 31, 52, 165, 182, 359
AlwI GGATC 1 cut(s) 438
Ama87I CYCGRG 2 cut(s) 221, 297
ApeKI GCWGC 1 cut(s) 324
ApoI RAATTY 1 cut(s) 248
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
AsuC2I CCSGG 2 cut(s) 222, 223
AsuHPI GGTGA 2 cut(s) 145, 157
AvaI CYCGRG 2 cut(s) 221, 297
BanII GRGCYC 1 cut(s) 222
BbsI GAAGAC 1 cut(s) 598
BbvI GCAGC 1 cut(s) 311
BccI CCATC 5 cut(s) 91, 200, 323, 341, 576
BclI TGATCA 1 cut(s) 135
BcnI CCSGG 2 cut(s) 222, 223
BcoDI GTCTC 5 cut(s) 31, 52, 165, 182, 359
BfaI CTAG 2 cut(s) 420, 540
BfmI CTRYAG 2 cut(s) 126, 240
BisI GCNGC 1 cut(s) 325
BlsI GCNGC 1 cut(s) 326
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 2 cut(s) 222, 223
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 2 cut(s) 221, 297
BmrFI CCNGG 2 cut(s) 222, 223
BmsI GCATC 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 598
BplI GAGNNNNNCTC 2 cut(s) 162, 194
BpuMI CCSGG 2 cut(s) 222, 223
BsaHI GRCGYC 1 cut(s) 595
BsaI GGTCTC 2 cut(s) 52, 165
BsaJI CCNNGG 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 2 cut(s) 100, 484
BseLI CCNNNNNNNGG 1 cut(s) 465
BseXI GCAGC 1 cut(s) 311
BsiHKCI CYCGRG 2 cut(s) 221, 297
BsiSI CCGG 2 cut(s) 222, 559
BslFI GGGAC 2 cut(s) 223, 238
BslI CCNNNNNNNGG 1 cut(s) 465
BsmAI GTCTC 5 cut(s) 31, 52, 165, 182, 359
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 223, 238
Bso31I GGTCTC 2 cut(s) 52, 165
BsoBI CYCGRG 2 cut(s) 221, 297
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 4 cut(s) 135, 307, 430, 581
BspACI CCGC 3 cut(s) 30, 384, 412
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 1 cut(s) 438
BspTNI GGTCTC 2 cut(s) 52, 165
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 4 cut(s) 135, 307, 430, 581
BssNI GRCGYC 1 cut(s) 595
Bst4CI ACNGT 3 cut(s) 176, 200, 282
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 244
BstF5I GGATG 2 cut(s) 100, 484
BstKTI GATC 4 cut(s) 138, 310, 433, 584
BstMAI GTCTC 5 cut(s) 31, 52, 165, 182, 359
BstMBI GATC 4 cut(s) 135, 307, 430, 581
BstMWI GCNNNNNNNGC 2 cut(s) 239, 381
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 2 cut(s) 220, 221
BstSFI CTRYAG 2 cut(s) 126, 240
BstV1I GCAGC 1 cut(s) 311
BstV2I GAAGAC 1 cut(s) 598
BtgZI GCGATG 1 cut(s) 342
BtsCI GGATG 2 cut(s) 100, 484
Cac8I GCNNGC 1 cut(s) 244
Cfr9I CCCGGG 1 cut(s) 221
Csp6I GTAC 4 cut(s) 118, 273, 397, 524
CviAII CATG 2 cut(s) 372, 379
CviJI RGCY 2 cut(s) 220, 496
CviKI_1 RGCY 2 cut(s) 220, 496
CviQI GTAC 4 cut(s) 118, 273, 397, 524
DpnI GATC 4 cut(s) 137, 309, 432, 583
DpnII GATC 4 cut(s) 135, 307, 430, 581
DriI GACNNNNNGTC 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 93
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 222
Eco31I GGTCTC 2 cut(s) 52, 165
Eco88I CYCGRG 2 cut(s) 221, 297
EcoT22I ATGCAT 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 222
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 2 cut(s) 375, 382
FaiI YATR 6 cut(s) 17, 19, 287, 373, 380, 441
FaqI GGGAC 2 cut(s) 223, 238
FatI CATG 2 cut(s) 371, 378
FauI CCCGC 1 cut(s) 377
FauNDI CATATG 1 cut(s) 17
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 1 cut(s) 325
FokI GGATG 2 cut(s) 87, 491
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 1 cut(s) 325
FspBI CTAG 2 cut(s) 420, 540
GluI GCNGC 1 cut(s) 325
HapII CCGG 2 cut(s) 222, 559
Hin1I GRCGYC 1 cut(s) 595
Hin1II CATG 2 cut(s) 375, 382
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 1 cut(s) 152
HpaII CCGG 2 cut(s) 222, 559
HphI GGTGA 2 cut(s) 145, 157
Hpy166II GTNNAC 3 cut(s) 165, 275, 445
Hpy188I TCNGA 2 cut(s) 261, 492
Hpy188III TCNNGA 4 cut(s) 297, 420, 428, 476
Hpy8I GTNNAC 3 cut(s) 165, 275, 445
Hpy99I CGWCG 1 cut(s) 576
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 3 cut(s) 176, 200, 282
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 3 cut(s) 144, 242, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 381
HpySE526I ACGT 1 cut(s) 595
Hsp92I GRCGYC 1 cut(s) 595
Hsp92II CATG 2 cut(s) 375, 382
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 4 cut(s) 135, 307, 430, 581
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 1 cut(s) 384
MaeI CTAG 2 cut(s) 420, 540
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 1 cut(s) 194
MalI GATC 4 cut(s) 137, 309, 432, 583
MboI GATC 4 cut(s) 135, 307, 430, 581
MboII GAAGA 4 cut(s) 80, 285, 520, 603
MfeI CAATTG 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 4 cut(s) 187, 248, 358, 527
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 2 cut(s) 124, 428
Mph1103I ATGCAT 1 cut(s) 377
MseI TTAA 3 cut(s) 303, 498, 604
MslI CAYNNNNRTG 1 cut(s) 573
MspI CCGG 2 cut(s) 222, 559
MspR9I CCNGG 2 cut(s) 222, 223
MunI CAATTG 1 cut(s) 358
MwoI GCNNNNNNNGC 2 cut(s) 239, 381
NciI CCSGG 2 cut(s) 222, 223
NdeI CATATG 1 cut(s) 17
NdeII GATC 4 cut(s) 135, 307, 430, 581
NlaIII CATG 2 cut(s) 375, 382
NmuCI GTSAC 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 377
NspI RCATGY 1 cut(s) 375
PaeR7I CTCGAG 1 cut(s) 297
PkrI GCNGC 1 cut(s) 326
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PstI CTGCAG 1 cut(s) 244
RsaI GTAC 4 cut(s) 119, 274, 398, 525
RsaNI GTAC 4 cut(s) 118, 273, 397, 524
RseI CAYNNNNRTG 1 cut(s) 573
SaqAI TTAA 3 cut(s) 303, 498, 604
SatI GCNGC 1 cut(s) 325
Sau3AI GATC 4 cut(s) 135, 307, 430, 581
SbfI CCTGCAGG 1 cut(s) 244
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 222, 223
SdaI CCTGCAGG 1 cut(s) 244
SduI GDGCHC 1 cut(s) 222
SetI ASST 4 cut(s) 135, 498, 505, 598
SfaNI GCATC 1 cut(s) 384
SfcI CTRYAG 2 cut(s) 126, 240
Sfr274I CTCGAG 1 cut(s) 297
SlaI CTCGAG 1 cut(s) 297
SmaI CCCGGG 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse8387I CCTGCAGG 1 cut(s) 244
Sse9I AATT 4 cut(s) 187, 248, 358, 527
SsiI CCGC 3 cut(s) 30, 384, 412
SspI AATATT 1 cut(s) 265
SspMI CTAG 2 cut(s) 420, 540
StyD4I CCNGG 2 cut(s) 220, 221
TaaI ACNGT 3 cut(s) 176, 200, 282
TaiI ACGT 1 cut(s) 598
TaqI TCGA 3 cut(s) 108, 298, 433
TasI AATT 4 cut(s) 187, 248, 358, 527
TatI WGTACW 3 cut(s) 117, 272, 396
Tru1I TTAA 3 cut(s) 303, 498, 604
Tru9I TTAA 3 cut(s) 303, 498, 604
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 324
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 5 cut(s) 4, 89, 126, 274, 475
TspMI CCCGGG 1 cut(s) 221
XapI RAATTY 1 cut(s) 248
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 1 cut(s) 375
XhoI CTCGAG 1 cut(s) 297
XmaI CCCGGG 1 cut(s) 221
XspI CTAG 2 cut(s) 420, 540
ZraI GACGTC 1 cut(s) 596
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.