MD17G1204100.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012640
Physical Location & Seq
Reverse (-)
24683338 .. 24686531
3194 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1204100.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGCCTAAAGTTAGAAGAGATAGTTCCTTGTCTCTTGATCGATCTAGGGCATCTCCTTATCCGTGCACCTCTGATGATTCTGATGCTTACAACCTGAAAAATCCTTTGGAAAGCAAAGATAACGCAAAAGAGTGGGAAGAAGCTAGGTGCCCCATCTGCATGGAACACGCACACAATGCTGTTCTTTTAAAATGCTCATCTCATGAGAAGGGCTGCAGGCCTTTCATGTGCAATACAAGCTATCGGCACTCAAATTGCTTTGACCAGTTTTGTAAGTCATCTTCGCCTTGCTGCTCAACAGTTATGTTGCAGGAACTTCCTCTCACACACACCTCTCACATTGCTAGCGAGGAACAGTCGTCGCCTGGGCAAAGCAGCCCCTGTGGCAGTCAATTGGATTTGAAGCCAGTTTGCCCTCTTTGTCGTGGAGAGATATATGGGTATGTTGTTGTAGAGGCTGCTCGCCACTTCATGAATGCCAAAGTGAGGAATTGCTCTTCTGAGACATGTGACTTTAGTGGGACTTATTCAGAACTGAGGAAGCATGCTAGGTCTGAACATCCTGCTGTCCGTCCATCCGAGGTGGATCCAAATCGGCATAGTGATTGGGTAAGGTTGGAGCGTGAAAGGGACTTGGAAGATGTTCTTAGCTTGGTCCAACAAGGACCCGGAGATGAACTTCTTGATGATTCATCAGGGGATCTTAATTCCTGGATGTCGACCGTGTTCGCTGCAATGTTTCGTTCTCTTGAGGTCATGCTTGTGACTCGTTTGATGGATTCTTCAAGTGGCTCTTCAAGTGGCAGAGAGCAGCAGACGCATAACCGCAGATCAGGAAGGACATCCAGGGTTCATTATGACAATGATACCATTCCTTCCACTAGACGGAGCAACAATGTATCAGATAGTACATCTCGTCCCAGACGTCTGCGTTGGCGGGCACTGAATGATGATGTGGGGACTAGTCGTTCCAGATGGGGTCGCAATTCCATGTCTGGGGAGGCTGGTGGTCATGCGCCTAGATGGGGCAACAGTTTGTTATCAGAGCAAAATAGCCGTACCCCTAGATGGGGCAACAACTCCTTGTCAGAGGAAGCTAACCATGCCCCCAGATGGGGCAACAATCCCTTATCAGAGGAGACCAGTCGCGCCTCTAGAATGGGCAACAACTCCTTAACGGAGGAGACCAGTCGTTGGCCTAGATGGGGCAACAACACCTTACCAGAAGAAAGCAGTCGTGCTTCTATATGGGGCAACAACTCCTTACGTGAAGAAACCAATCGTGCTCCTAGATGGGGAAACAACTCCTTAACTGAAGAGACCAGTCGTGCTCCTAGATGGGGCAATGCTTCAGCACCTGAAAATACCCCTCGTACTGGAAGGCTGCGCTGGAGAAATCAAAGATGGCCAACAAATAACAGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

476

Amino Acids

53.79

Weight (kDa)

8.61

Isoelectric Point (pI)

74.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 46 - 217 3.1e-65 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 928
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 1194
AccI GTMKAC 1 cut(s) 719
AccII CGCG 1 cut(s) 1149
AciI CCGC 3 cut(s) 826, 937, 1423
AclWI GGATC 3 cut(s) 581, 594, 708
AcoI YGGCCR 1 cut(s) 1406
AcuI CTGAAG 2 cut(s) 1335, 1335
AcyI GRCGYC 1 cut(s) 925
AfaI GTAC 3 cut(s) 910, 1060, 1375
AflIII ACRYGT 1 cut(s) 506
AgsI TTSAA 3 cut(s) 403, 786, 798
AhlI ACTAGT 1 cut(s) 962
AjnI CCWGG 3 cut(s) 364, 710, 845
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AloI GAACNNNNNNTCC 2 cut(s) 952, 984
AluBI AGCT 4 cut(s) 143, 240, 651, 1097
AluI AGCT 4 cut(s) 143, 240, 651, 1097
Alw21I GWGCWC 3 cut(s) 68, 1288, 1333
Alw26I GTCTC 5 cut(s) 36, 497, 1133, 1178, 1313
Alw44I GTGCAC 1 cut(s) 64
AlwI GGATC 3 cut(s) 581, 594, 708
AlwNI CAGNNNCTG 2 cut(s) 381, 1358
AoxI GGCC 3 cut(s) 218, 1196, 1406
ApaLI GTGCAC 1 cut(s) 64
ApeKI GCWGC 7 cut(s) 213, 291, 375, 458, 731, 811, 1384
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 3 cut(s) 1018, 1151, 1389
AspS9I GGNCC 2 cut(s) 655, 665
AsuC2I CCSGG 1 cut(s) 669
AsuNHI GCTAGC 1 cut(s) 344
AvaII GGWCC 2 cut(s) 655, 665
BaeGI GKGCMC 3 cut(s) 68, 152, 943
BalI TGGCCA 1 cut(s) 1408
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 1 cut(s) 147
BarI GAAGNNNNNNTAC 2 cut(s) 265, 297
Bbv12I GWGCWC 3 cut(s) 68, 1288, 1333
BbvI GCAGC 7 cut(s) 200, 278, 387, 445, 718, 823, 1371
BceAI ACGGC 1 cut(s) 1041
BciT130I CCWGG 3 cut(s) 366, 712, 847
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 5 cut(s) 36, 497, 1133, 1178, 1313
BcuI ACTAGT 1 cut(s) 962
BfmI CTRYAG 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 384
BisI GCNGC 7 cut(s) 214, 292, 376, 459, 732, 812, 1385
BlsI GCNGC 7 cut(s) 215, 293, 377, 460, 733, 813, 1386
Bme1390I CCNGG 4 cut(s) 366, 669, 712, 847
Bme18I GGWCC 2 cut(s) 655, 665
BmgT120I GGNCC 2 cut(s) 655, 665
BmiI GGNNCC 3 cut(s) 149, 588, 667
BmrFI CCNGG 4 cut(s) 366, 669, 712, 847
BmsI GCATC 2 cut(s) 59, 73
BmtI GCTAGC 1 cut(s) 348
BpmI CTGGAG 1 cut(s) 1411
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 1 cut(s) 669
Bsa29I ATCGAT 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 1268
BsaBI GATNNNNATC 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 925
BsaI GGTCTC 3 cut(s) 1133, 1178, 1313
BsaJI CCNNGG 3 cut(s) 365, 579, 846
Bse1I ACTGG 6 cut(s) 265, 407, 1143, 1188, 1323, 1381
Bse3DI GCAATG 3 cut(s) 339, 741, 1351
Bse8I GATNNNNATC 1 cut(s) 591
BseBI CCWGG 3 cut(s) 366, 712, 847
BseCI ATCGAT 1 cut(s) 40
BseDI CCNNGG 3 cut(s) 365, 579, 846
BseGI GGATG 4 cut(s) 559, 575, 720, 842
BseJI GATNNNNATC 1 cut(s) 591
BseMI GCAATG 3 cut(s) 339, 741, 1351
BseMII CTCAG 2 cut(s) 492, 527
BseNI ACTGG 6 cut(s) 265, 407, 1143, 1188, 1323, 1381
BseRI GAGGAG 2 cut(s) 1151, 1196
BseSI GKGCMC 3 cut(s) 68, 152, 943
BseXI GCAGC 7 cut(s) 200, 278, 387, 445, 718, 823, 1371
Bsh1236I CGCG 1 cut(s) 1149
Bsh1285I CGRYCG 1 cut(s) 723
BshFI GGCC 3 cut(s) 220, 1198, 1408
BshNI GGYRCC 1 cut(s) 147
BshVI ATCGAT 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 723
BsiHKAI GWGCWC 3 cut(s) 68, 1288, 1333
BsiSI CCGG 1 cut(s) 669
BslFI GGGAC 4 cut(s) 535, 644, 903, 973
BsmAI GTCTC 5 cut(s) 36, 497, 1133, 1178, 1313
BsmFI GGGAC 4 cut(s) 535, 644, 903, 973
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 3 cut(s) 220, 1198, 1408
Bso31I GGTCTC 3 cut(s) 1133, 1178, 1313
Bsp1286I GDGCHC 5 cut(s) 68, 152, 943, 1288, 1333
Bsp143I GATC 5 cut(s) 37, 41, 586, 700, 830
BspACI CCGC 3 cut(s) 826, 937, 1423
BspANI GGCC 3 cut(s) 220, 1198, 1408
BspCNI CTCAG 2 cut(s) 493, 528
BspDI ATCGAT 1 cut(s) 40
BspFNI CGCG 1 cut(s) 1149
BspHI TCATGA 2 cut(s) 202, 471
BspLI GGNNCC 3 cut(s) 149, 588, 667
BspMAI CTGCAG 1 cut(s) 218
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 3 cut(s) 581, 594, 708
BspQI GCTCTTC 2 cut(s) 502, 799
BspT107I GGYRCC 1 cut(s) 147
BspTNI GGTCTC 3 cut(s) 1133, 1178, 1313
BsrDI GCAATG 3 cut(s) 339, 741, 1351
BsrI ACTGG 6 cut(s) 265, 407, 1143, 1188, 1323, 1381
BssECI CCNNGG 3 cut(s) 365, 579, 846
BssMI GATC 5 cut(s) 37, 41, 586, 700, 830
BssNI GRCGYC 1 cut(s) 925
Bst2UI CCWGG 3 cut(s) 366, 712, 847
Bst4CI ACNGT 4 cut(s) 301, 357, 724, 1034
Bst6I CTCTTC 4 cut(s) 10, 502, 799, 1311
BstACI GRCGYC 1 cut(s) 925
BstAPI GCANNNNNTGC 1 cut(s) 176
BstBAI YACGTR 1 cut(s) 1268
BstC8I GCNNGC 5 cut(s) 218, 346, 463, 546, 939
BstDEI CTNAG 3 cut(s) 501, 536, 647
BstF5I GGATG 4 cut(s) 559, 575, 720, 842
BstFNI CGCG 1 cut(s) 1149
BstHHI GCGC 3 cut(s) 1018, 1151, 1389
BstKTI GATC 5 cut(s) 40, 44, 589, 703, 833
BstMAI GTCTC 5 cut(s) 36, 497, 1133, 1178, 1313
BstMBI GATC 5 cut(s) 37, 41, 586, 700, 830
BstMCI CGRYCG 1 cut(s) 723
BstMWI GCNNNNNNNGC 6 cut(s) 156, 176, 237, 384, 817, 1103
BstNI CCWGG 3 cut(s) 366, 712, 847
BstNSI RCATGY 2 cut(s) 510, 548
BstSCI CCNGG 4 cut(s) 364, 667, 710, 845
BstSFI CTRYAG 1 cut(s) 214
BstSLI GKGCMC 3 cut(s) 68, 152, 943
BstUI CGCG 1 cut(s) 1149
BstV1I GCAGC 7 cut(s) 200, 278, 387, 445, 718, 823, 1371
BstX2I RGATCY 2 cut(s) 586, 700
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 2 cut(s) 586, 700
Bsu15I ATCGAT 1 cut(s) 40
BsuRI GGCC 3 cut(s) 220, 1198, 1408
BsuTUI ATCGAT 1 cut(s) 40
BtsCI GGATG 4 cut(s) 559, 575, 720, 842
BtsIMutI CAGTG 1 cut(s) 941
Cac8I GCNNGC 5 cut(s) 218, 346, 463, 546, 939
CaiI CAGNNNCTG 2 cut(s) 381, 1358
CciI TCATGA 2 cut(s) 202, 471
CfoI GCGC 3 cut(s) 1018, 1151, 1389
Cfr13I GGNCC 2 cut(s) 655, 665
ClaI ATCGAT 1 cut(s) 40
CseI GACGC 1 cut(s) 826
Csp6I GTAC 3 cut(s) 909, 1059, 1374
CviQI GTAC 3 cut(s) 909, 1059, 1374
DdeI CTNAG 3 cut(s) 501, 536, 647
DpnI GATC 5 cut(s) 39, 43, 588, 702, 832
DpnII GATC 5 cut(s) 37, 41, 586, 700, 830
DraI TTTAAA 1 cut(s) 189
EaeI YGGCCR 1 cut(s) 1406
Eam1104I CTCTTC 4 cut(s) 10, 502, 799, 1311
EarI CTCTTC 4 cut(s) 10, 502, 799, 1311
Eco147I AGGCCT 1 cut(s) 220
Eco31I GGTCTC 3 cut(s) 1133, 1178, 1313
Eco47I GGWCC 2 cut(s) 655, 665
Eco57I CTGAAG 2 cut(s) 1335, 1335
EcoO109I RGGNCCY 1 cut(s) 665
EcoRII CCWGG 3 cut(s) 364, 710, 845
FalI AAGNNNNNCTT 4 cut(s) 630, 662, 778, 810
FaqI GGGAC 4 cut(s) 535, 644, 903, 973
FauI CCCGC 1 cut(s) 930
FblI GTMKAC 1 cut(s) 719
Fnu4HI GCNGC 7 cut(s) 214, 292, 376, 459, 732, 812, 1385
FokI GGATG 4 cut(s) 546, 562, 727, 829
Fsp4HI GCNGC 7 cut(s) 214, 292, 376, 459, 732, 812, 1385
GlaI GCGC 3 cut(s) 1017, 1150, 1388
GluI GCNGC 7 cut(s) 214, 292, 376, 459, 732, 812, 1385
GsuI CTGGAG 1 cut(s) 1411
HaeIII GGCC 3 cut(s) 220, 1198, 1408
HapII CCGG 1 cut(s) 669
HgaI GACGC 1 cut(s) 826
HhaI GCGC 3 cut(s) 1018, 1151, 1389
Hin1I GRCGYC 1 cut(s) 925
Hin6I GCGC 3 cut(s) 1016, 1149, 1387
HinP1I GCGC 3 cut(s) 1016, 1149, 1387
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HinfI GANTC 4 cut(s) 77, 689, 766, 779
HpaII CCGG 1 cut(s) 669
Hpy166II GTNNAC 2 cut(s) 66, 720
Hpy188III TCNNGA 8 cut(s) 35, 203, 472, 683, 749, 834, 972, 1155
Hpy8I GTNNAC 2 cut(s) 66, 720
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 4 cut(s) 202, 831, 885, 1374
HpyCH4III ACNGT 4 cut(s) 301, 357, 724, 1034
HpyCH4IV ACGT 2 cut(s) 925, 1267
HpyCH4V TGCA 6 cut(s) 66, 159, 216, 231, 310, 734
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 176, 237, 384, 817, 1103
HpyF3I CTNAG 3 cut(s) 501, 536, 647
HpySE526I ACGT 2 cut(s) 925, 1267
Hsp92I GRCGYC 1 cut(s) 925
HspAI GCGC 3 cut(s) 1016, 1149, 1387
Kzo9I GATC 5 cut(s) 37, 41, 586, 700, 830
LguI GCTCTTC 2 cut(s) 502, 799
LmnI GCTCC 4 cut(s) 619, 888, 1291, 1336
Lsp1109I GCAGC 7 cut(s) 200, 278, 387, 445, 718, 823, 1371
LweI GCATC 2 cut(s) 59, 73
MaeII ACGT 2 cut(s) 925, 1267
MaeIII GTNAC 2 cut(s) 509, 763
MalI GATC 5 cut(s) 39, 43, 588, 702, 832
MboI GATC 5 cut(s) 37, 41, 586, 700, 830
MfeI CAATTG 1 cut(s) 392
MflI RGATCY 2 cut(s) 586, 700
MhlI GDGCHC 5 cut(s) 68, 152, 943, 1288, 1333
MlsI TGGCCA 1 cut(s) 1408
MluCI AATT 5 cut(s) 253, 392, 490, 706, 985
MluNI TGGCCA 1 cut(s) 1408
MlyI GAGTC 1 cut(s) 760
MmeI TCCRAC 2 cut(s) 597, 682
Mox20I TGGCCA 1 cut(s) 1408
MroXI GAANNNNTTC 1 cut(s) 642
MscI TGGCCA 1 cut(s) 1408
MseI TTAA 4 cut(s) 188, 705, 1175, 1310
MslI CAYNNNNRTG 2 cut(s) 158, 761
Msp20I TGGCCA 1 cut(s) 1408
MspI CCGG 1 cut(s) 669
MspR9I CCNGG 4 cut(s) 366, 669, 712, 847
MunI CAATTG 1 cut(s) 392
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 3 cut(s) 366, 712, 847
MvnI CGCG 1 cut(s) 1149
MwoI GCNNNNNNNGC 6 cut(s) 156, 176, 237, 384, 817, 1103
NciI CCSGG 1 cut(s) 669
NdeII GATC 5 cut(s) 37, 41, 586, 700, 830
NheI GCTAGC 1 cut(s) 344
NlaIV GGNNCC 3 cut(s) 149, 588, 667
NmuCI GTSAC 2 cut(s) 509, 763
NspI RCATGY 2 cut(s) 510, 548
PaeI GCATGC 1 cut(s) 548
PagI TCATGA 2 cut(s) 202, 471
PceI AGGCCT 1 cut(s) 220
PciI ACATGT 1 cut(s) 506
PciSI GCTCTTC 2 cut(s) 502, 799
PcsI WCGNNNNNNNCGW 1 cut(s) 922
PctI GAATGC 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 3 cut(s) 77, 689, 779
PflMI CCANNNNNTGG 1 cut(s) 1194
PfoI TCCNGGA 1 cut(s) 710
PkrI GCNGC 7 cut(s) 215, 293, 377, 460, 733, 813, 1386
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
Ppu21I YACGTR 1 cut(s) 1268
PpuMI RGGWCCY 1 cut(s) 665
PscI ACATGT 1 cut(s) 506
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 3 cut(s) 364, 710, 845
PspGI CCWGG 3 cut(s) 364, 710, 845
PspN4I GGNNCC 3 cut(s) 149, 588, 667
PspPI GGNCC 2 cut(s) 655, 665
PspPPI RGGWCCY 1 cut(s) 665
PstI CTGCAG 1 cut(s) 218
PstNI CAGNNNCTG 2 cut(s) 381, 1358
PsuI RGATCY 2 cut(s) 586, 700
RsaI GTAC 3 cut(s) 910, 1060, 1375
RsaNI GTAC 3 cut(s) 909, 1059, 1374
RseI CAYNNNNRTG 2 cut(s) 158, 761
SalI GTCGAC 1 cut(s) 718
SapI GCTCTTC 2 cut(s) 502, 799
SaqAI TTAA 4 cut(s) 188, 705, 1175, 1310
SatI GCNGC 7 cut(s) 214, 292, 376, 459, 732, 812, 1385
Sau3AI GATC 5 cut(s) 37, 41, 586, 700, 830
Sau96I GGNCC 2 cut(s) 655, 665
SchI GAGTC 1 cut(s) 760
ScrFI CCNGG 4 cut(s) 366, 669, 712, 847
SduI GDGCHC 5 cut(s) 68, 152, 943, 1288, 1333
SfaNI GCATC 2 cut(s) 59, 73
SfcI CTRYAG 1 cut(s) 214
SinI GGWCC 2 cut(s) 655, 665
SmiMI CAYNNNNRTG 2 cut(s) 158, 761
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
SpeI ACTAGT 1 cut(s) 962
SphI GCATGC 1 cut(s) 548
Sse9I AATT 5 cut(s) 253, 392, 490, 706, 985
SseBI AGGCCT 1 cut(s) 220
SsiI CCGC 3 cut(s) 826, 937, 1423
StuI AGGCCT 1 cut(s) 220
StyD4I CCNGG 4 cut(s) 364, 667, 710, 845
TaaI ACNGT 4 cut(s) 301, 357, 724, 1034
TaiI ACGT 2 cut(s) 928, 1270
TaqI TCGA 2 cut(s) 40, 719
TasI AATT 5 cut(s) 253, 392, 490, 706, 985
TatI WGTACW 1 cut(s) 908
TfiI GAWTC 3 cut(s) 77, 689, 779
Tru1I TTAA 4 cut(s) 188, 705, 1175, 1310
Tru9I TTAA 4 cut(s) 188, 705, 1175, 1310
TscAI CASTG 1 cut(s) 948
TseFI GTSAC 2 cut(s) 509, 763
TseI GCWGC 7 cut(s) 213, 291, 375, 458, 731, 811, 1384
Tsp45I GTSAC 2 cut(s) 509, 763
TspDTI ATGAA 6 cut(s) 214, 460, 488, 681, 690, 842
TspGWI ACGGA 4 cut(s) 51, 560, 901, 1193
TspRI CASTG 1 cut(s) 948
Van91I CCANNNNNTGG 1 cut(s) 1194
VneI GTGCAC 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 655, 665
XbaI TCTAGA 1 cut(s) 1154
XceI RCATGY 2 cut(s) 510, 548
XmiI GTMKAC 1 cut(s) 719
XmnI GAANNNNTTC 1 cut(s) 642
ZraI GACGTC 1 cut(s) 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.