RLG00000019562

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012640
Physical Location & Seq
Forward (+)
53610820 .. 53614461
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019562

Sequence Viewer

Length: 1536 bp
ATGATGCAGCTAACCCACGCCCTTGTTTCCATTTTGCTCTCTCTCAAAGTTGCCCAAGTCCTCCCCACAACAACAACAGAAGACCTCTCCTCCGGTGTTGAAAAAGGTAGTTTTAGTATTCTCGGGTGCTATCTTAACTTCAAAATGCCCAAAGTCAGACGATCATCTTCCTTGTCTCTTGATCGATCCAGGGCATCACCTTATCCTTGCACCTCTGAGGATGCTGAGCAATACAAGTTGAATAGTCCGTTAGAAAGCACAGAGAATGCAAAAGAATGGGAAGATACTAGATGCCCCATCTGCATGGAACACCCACATAATGCTGTTCTTATAAAATGTTCCTCTCATGAGATGGGCTGCAGGCCGTACATGTGCAATACAAGCTACCGGCACTCAAATTGTCTTGACCAGTTTTGTAAGTCTTCTGTTCCTGAGTCCACAACAATGATGTTGCAAGAACTTCCTCTGACACACACAGCCTCTCACGGTTCTAGGGAAGAAGAATCGCTGCCTGGGCAAACCAGCCCTTGTGGGAGTCAGTTGCAACCACATCCAGCTTGCCCTCTTTGTCGGGGAAAGATCTATGGGTATATTGTTGTAGAGGCTGCTCGTCACTTCATGAATTGCAAAGTGAGGAGCTGCTCTTCTGAGACGTGCGACTTTAATGGAACTTATTCAGAACTGAGGAAGCATGCGAGGTCTGAACACCCTTATGTCCGGCCATCAGAGGTGGATCCAAATCGTCATCGTGATTGGATGAGATTGGAACGTCAAAGGGACTTGCAAGATGTACTTAGCATAGTCCAAGTAGGGTCTGGAGACGATATTATTCCGGATCCATCAACAGACGGTGGCTGGATGACTTCCCTTTTAAGTGCTATGTTTCGCTCGTTTGAGATTATGATTTTTTCTCAATTGATGGAGAGTTCAAGTGACATGGAACAAACTCATAGTAGGAGATCAGGGAGAATGACTAGGGTCCAGCAAGACAATGACACAATCCCTGCTACTAGAAGGAACAACACTGTGTCAGATAGCACATCCCGTCCCAGAGGTCTACGTTGGCGAGCACCAAATAATAATGTCGAGACAGCTCGTACCTCCAGATTGAGCCACAATTCCTTGTCAGGGGAGACTAGCCATACCTCTAGATGGAGCAACAACTCATTGGAGGAGGCTAGCCGTGCCCCTAGATGGGGTAACTCCTTATCTGAGGAGGGTACTAACCGTGTCCTTAGAAGGGGTAACAACGGCGTATCAGACACTACCCGTCCCTCCAGATGGGGCAACAACTCTGTGCCAGAGGAGAGTAATCGTGTCCACAGATGGGGTAACAACTCCGTGCCAGAGAGTAATCGTGTCCACAGATGGGGCAACAACTCCTTATCAGAGGAGAGTAGATGGGGCAACAACTCCTTATCGGAAGAGGCTGGGCATGCCCCTAGATGGGGCAATACTTCTGTACCAGAAAGCACACCCCGTCCTCGGAGACTGAGATGGAGAAATCAATGGCCAACGTACAATAACAGAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

512

Amino Acids

58.03

Weight (kDa)

8.7

Isoelectric Point (pI)

68.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 94 - 266 4.5e-67 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
AccI GTMKAC 1 cut(s) 1057
AccIII TCCGGA 1 cut(s) 832
AclWI GGATC 5 cut(s) 180, 728, 741, 830, 843
AcoI YGGCCR 2 cut(s) 719, 1511
AfaI GTAC 6 cut(s) 368, 792, 1099, 1222, 1464, 1520
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 4 cut(s) 101, 142, 241, 930
AjiI CACGTC 1 cut(s) 654
AjnI CCWGG 2 cut(s) 188, 511
AluBI AGCT 5 cut(s) 10, 384, 557, 639, 1094
AluI AGCT 5 cut(s) 10, 384, 557, 639, 1094
Alw21I GWGCWC 1 cut(s) 1072
Alw26I GTCTC 6 cut(s) 180, 644, 813, 1082, 1127, 1483
AlwI GGATC 5 cut(s) 180, 728, 741, 830, 843
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 832
AoxI GGCC 3 cut(s) 362, 719, 1511
ApeKI GCWGC 5 cut(s) 7, 357, 508, 605, 639
Asp700I GAANNNNTTC 1 cut(s) 673
AspS9I GGNCC 1 cut(s) 979
AsuHPI GGTGA 1 cut(s) 189
AsuNHI GCTAGC 1 cut(s) 1178
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 1 cut(s) 979
BaeGI GKGCMC 1 cut(s) 1189
BaeI ACNNNNGTAYC 2 cut(s) 1446, 1479
BalI TGGCCA 1 cut(s) 1513
BamHI GGATCC 2 cut(s) 733, 835
BbsI GAAGAC 2 cut(s) 87, 414
Bbv12I GWGCWC 1 cut(s) 1072
BbvI GCAGC 5 cut(s) 19, 344, 495, 592, 626
BceAI ACGGC 3 cut(s) 349, 1167, 1267
BciT130I CCWGG 2 cut(s) 190, 513
BcoDI GTCTC 6 cut(s) 180, 644, 813, 1082, 1127, 1483
BfaI CTAG 9 cut(s) 288, 492, 975, 1011, 1137, 1149, 1179, 1191, 1443
BfmI CTRYAG 1 cut(s) 358
BglII AGATCT 1 cut(s) 579
BisI GCNGC 5 cut(s) 8, 358, 509, 606, 640
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 5 cut(s) 9, 359, 510, 607, 641
Bme1390I CCNGG 2 cut(s) 190, 513
Bme18I GGWCC 1 cut(s) 979
BmeT110I CYCGRG 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 654
BmgT120I GGNCC 1 cut(s) 979
BmiI GGNNCC 3 cut(s) 735, 837, 980
BmrFI CCNGG 2 cut(s) 190, 513
BmsI GCATC 3 cut(s) 203, 211, 281
BmtI GCTAGC 1 cut(s) 1182
BoxI GACNNNNGTC 1 cut(s) 977
BpiI GAAGAC 2 cut(s) 87, 414
BpmI CTGGAG 3 cut(s) 837, 1087, 1261
Bpu1102I GCTNAGC 1 cut(s) 225
Bsa29I ATCGAT 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 738
BsaJI CCNNGG 3 cut(s) 189, 512, 1484
BsaWI WCCGGW 2 cut(s) 92, 832
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse118I RCCGGY 1 cut(s) 387
Bse1I ACTGG 1 cut(s) 409
Bse8I GATNNNNATC 1 cut(s) 738
BseAI TCCGGA 1 cut(s) 832
BseBI CCWGG 2 cut(s) 190, 513
BseCI ATCGAT 1 cut(s) 184
BseDI CCNNGG 3 cut(s) 189, 512, 1484
BseGI GGATG 5 cut(s) 226, 550, 762, 864, 1040
BseJI GATNNNNATC 1 cut(s) 738
BseMII CTCAG 7 cut(s) 207, 216, 423, 639, 674, 1203, 1484
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 6 cut(s) 79, 649, 1187, 1229, 1319, 1406
BseSI GKGCMC 1 cut(s) 1189
BseXI GCAGC 5 cut(s) 19, 344, 495, 592, 626
BseYI CCCAGC 1 cut(s) 1430
BshFI GGCC 3 cut(s) 364, 721, 1513
BshVI ATCGAT 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 1072
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 4 cut(s) 93, 388, 718, 833
BslFI GGGAC 3 cut(s) 791, 1032, 1257
BsmAI GTCTC 6 cut(s) 180, 644, 813, 1082, 1127, 1483
BsmBI CGTCTC 2 cut(s) 644, 813
BsmFI GGGAC 3 cut(s) 791, 1032, 1257
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 3 cut(s) 364, 721, 1513
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 1072, 1189
Bsp13I TCCGGA 1 cut(s) 832
Bsp143I GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
Bsp1720I GCTNAGC 1 cut(s) 225
BspANI GGCC 3 cut(s) 364, 721, 1513
BspCNI CTCAG 7 cut(s) 208, 217, 424, 640, 675, 1204, 1485
BspDI ATCGAT 1 cut(s) 184
BspEI TCCGGA 1 cut(s) 832
BspHI TCATGA 2 cut(s) 346, 618
BspLI GGNNCC 3 cut(s) 735, 837, 980
BspMAI CTGCAG 1 cut(s) 362
BspOI GCTAGC 1 cut(s) 1182
BspPI GGATC 5 cut(s) 180, 728, 741, 830, 843
BspQI GCTCTTC 1 cut(s) 649
BsrFI RCCGGY 1 cut(s) 387
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 387
BssECI CCNNGG 3 cut(s) 189, 512, 1484
BssMI GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
Bst2UI CCWGG 2 cut(s) 190, 513
Bst4CI ACNGT 4 cut(s) 488, 851, 1027, 1229
Bst6I CTCTTC 2 cut(s) 649, 1419
BstC8I GCNNGC 6 cut(s) 362, 559, 693, 1068, 1180, 1437
BstDEI CTNAG 9 cut(s) 216, 225, 432, 648, 683, 794, 1212, 1235, 1493
BstF5I GGATG 5 cut(s) 226, 550, 762, 864, 1040
BstKTI GATC 7 cut(s) 164, 184, 188, 582, 736, 838, 962
BstMAI GTCTC 6 cut(s) 180, 644, 813, 1082, 1127, 1483
BstMBI GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
BstMWI GCNNNNNNNGC 5 cut(s) 300, 381, 514, 1184, 1436
BstNI CCWGG 2 cut(s) 190, 513
BstNSI RCATGY 3 cut(s) 373, 695, 1439
BstPAI GACNNNNGTC 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 188, 511
BstSFI CTRYAG 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 1189
BstV1I GCAGC 5 cut(s) 19, 344, 495, 592, 626
BstV2I GAAGAC 2 cut(s) 87, 414
BstX2I RGATCY 3 cut(s) 579, 733, 835
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 3 cut(s) 579, 733, 835
Bsu15I ATCGAT 1 cut(s) 184
BsuRI GGCC 3 cut(s) 364, 721, 1513
BsuTUI ATCGAT 1 cut(s) 184
BtrI CACGTC 1 cut(s) 654
BtsCI GGATG 5 cut(s) 226, 550, 762, 864, 1040
BtsIMutI CAGTG 1 cut(s) 1023
Cac8I GCNNGC 6 cut(s) 362, 559, 693, 1068, 1180, 1437
CciI TCATGA 2 cut(s) 346, 618
Cfr10I RCCGGY 1 cut(s) 387
Cfr13I GGNCC 1 cut(s) 979
ClaI ATCGAT 1 cut(s) 184
Csp6I GTAC 6 cut(s) 367, 791, 1098, 1221, 1463, 1519
CviAII CATG 7 cut(s) 304, 347, 370, 619, 692, 937, 1436
CviQI GTAC 6 cut(s) 367, 791, 1098, 1221, 1463, 1519
DdeI CTNAG 9 cut(s) 216, 225, 432, 648, 683, 794, 1212, 1235, 1493
DpnI GATC 7 cut(s) 163, 183, 187, 581, 735, 837, 961
DpnII GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
EaeI YGGCCR 2 cut(s) 719, 1511
Eam1104I CTCTTC 2 cut(s) 649, 1419
EarI CTCTTC 2 cut(s) 649, 1419
Eco47I GGWCC 1 cut(s) 979
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 188, 511
Esp3I CGTCTC 2 cut(s) 644, 813
FaeI CATG 7 cut(s) 307, 350, 373, 622, 695, 940, 1439
FalI AAGNNNNNCTT 2 cut(s) 777, 809
FaqI GGGAC 3 cut(s) 791, 1032, 1257
FatI CATG 7 cut(s) 303, 346, 369, 618, 691, 936, 1435
FblI GTMKAC 1 cut(s) 1057
Fnu4HI GCNGC 5 cut(s) 8, 358, 509, 606, 640
FokI GGATG 5 cut(s) 233, 537, 769, 871, 1027
Fsp4HI GCNGC 5 cut(s) 8, 358, 509, 606, 640
FspBI CTAG 9 cut(s) 288, 492, 975, 1011, 1137, 1149, 1179, 1191, 1443
GluI GCNGC 5 cut(s) 8, 358, 509, 606, 640
GsaI CCCAGC 1 cut(s) 1434
GsuI CTGGAG 3 cut(s) 837, 1087, 1261
HaeIII GGCC 3 cut(s) 364, 721, 1513
HapII CCGG 4 cut(s) 93, 388, 718, 833
Hin1II CATG 7 cut(s) 307, 350, 373, 622, 695, 940, 1439
HinfI GANTC 3 cut(s) 434, 503, 535
HpaII CCGG 4 cut(s) 93, 388, 718, 833
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 4 cut(s) 438, 1058, 1321, 1363
Hpy8I GTNNAC 4 cut(s) 438, 1058, 1321, 1363
HpyAV CCTTC 3 cut(s) 1008, 1233, 1524
HpyCH4III ACNGT 4 cut(s) 488, 851, 1027, 1229
HpyCH4IV ACGT 4 cut(s) 653, 769, 1060, 1517
HpyF10VI GCNNNNNNNGC 5 cut(s) 300, 381, 514, 1184, 1436
HpyF3I CTNAG 9 cut(s) 216, 225, 432, 648, 683, 794, 1212, 1235, 1493
HpySE526I ACGT 4 cut(s) 653, 769, 1060, 1517
Hsp92II CATG 7 cut(s) 307, 350, 373, 622, 695, 940, 1439
Kpn2I TCCGGA 1 cut(s) 832
Kzo9I GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
LguI GCTCTTC 1 cut(s) 649
LmnI GCTCC 2 cut(s) 636, 1155
Lsp1109I GCAGC 5 cut(s) 19, 344, 495, 592, 626
LweI GCATC 3 cut(s) 203, 211, 281
MaeI CTAG 9 cut(s) 288, 492, 975, 1011, 1137, 1149, 1179, 1191, 1443
MaeII ACGT 4 cut(s) 653, 769, 1060, 1517
MaeIII GTNAC 5 cut(s) 611, 932, 1199, 1244, 1331
MalI GATC 7 cut(s) 163, 183, 187, 581, 735, 837, 961
MboI GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
MboII GAAGA 8 cut(s) 92, 159, 293, 414, 509, 512, 636, 1436
MfeI CAATTG 1 cut(s) 914
MflI RGATCY 3 cut(s) 579, 733, 835
MhlI GDGCHC 2 cut(s) 1072, 1189
MlsI TGGCCA 1 cut(s) 1513
MluCI AATT 4 cut(s) 397, 622, 914, 1117
MluNI TGGCCA 1 cut(s) 1513
MlyI GAGTC 2 cut(s) 443, 544
Mox20I TGGCCA 1 cut(s) 1513
MroI TCCGGA 1 cut(s) 832
MroXI GAANNNNTTC 1 cut(s) 673
MscI TGGCCA 1 cut(s) 1513
MseI TTAA 3 cut(s) 135, 663, 872
MslI CAYNNNNRTG 3 cut(s) 302, 443, 711
Msp20I TGGCCA 1 cut(s) 1513
MspI CCGG 4 cut(s) 93, 388, 718, 833
MspR9I CCNGG 2 cut(s) 190, 513
MunI CAATTG 1 cut(s) 914
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 2 cut(s) 190, 513
MwoI GCNNNNNNNGC 5 cut(s) 300, 381, 514, 1184, 1436
NdeII GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
NheI GCTAGC 1 cut(s) 1178
NlaIII CATG 7 cut(s) 307, 350, 373, 622, 695, 940, 1439
NlaIV GGNNCC 3 cut(s) 735, 837, 980
NmuCI GTSAC 2 cut(s) 611, 932
NspI RCATGY 3 cut(s) 373, 695, 1439
PaeI GCATGC 2 cut(s) 695, 1439
PagI TCATGA 2 cut(s) 346, 618
PciI ACATGT 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 649
PctI GAATGC 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 673
PfeI GAWTC 1 cut(s) 503
PkrI GCNGC 5 cut(s) 9, 359, 510, 607, 641
PleI GAGTC 2 cut(s) 442, 543
PpsI GAGTC 2 cut(s) 442, 543
PscI ACATGT 1 cut(s) 369
PshAI GACNNNNGTC 1 cut(s) 977
PsiI TTATAA 1 cut(s) 332
Psp6I CCWGG 2 cut(s) 188, 511
PspFI CCCAGC 1 cut(s) 1430
PspGI CCWGG 2 cut(s) 188, 511
PspN4I GGNNCC 3 cut(s) 735, 837, 980
PspPI GGNCC 1 cut(s) 979
PstI CTGCAG 1 cut(s) 362
PsuI RGATCY 3 cut(s) 579, 733, 835
RsaI GTAC 6 cut(s) 368, 792, 1099, 1222, 1464, 1520
RsaNI GTAC 6 cut(s) 367, 791, 1098, 1221, 1463, 1519
RseI CAYNNNNRTG 3 cut(s) 302, 443, 711
SapI GCTCTTC 1 cut(s) 649
SaqAI TTAA 3 cut(s) 135, 663, 872
SatI GCNGC 5 cut(s) 8, 358, 509, 606, 640
Sau3AI GATC 7 cut(s) 161, 181, 185, 579, 733, 835, 959
Sau96I GGNCC 1 cut(s) 979
SchI GAGTC 2 cut(s) 443, 544
ScrFI CCNGG 2 cut(s) 190, 513
SduI GDGCHC 2 cut(s) 1072, 1189
SfaNI GCATC 3 cut(s) 203, 211, 281
SfcI CTRYAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 979
SmiMI CAYNNNNRTG 3 cut(s) 302, 443, 711
SphI GCATGC 2 cut(s) 695, 1439
Sse9I AATT 4 cut(s) 397, 622, 914, 1117
SspMI CTAG 9 cut(s) 288, 492, 975, 1011, 1137, 1149, 1179, 1191, 1443
StyD4I CCNGG 2 cut(s) 188, 511
TaaI ACNGT 4 cut(s) 488, 851, 1027, 1229
TaiI ACGT 4 cut(s) 656, 772, 1063, 1520
TaqI TCGA 2 cut(s) 184, 1086
TasI AATT 4 cut(s) 397, 622, 914, 1117
TatI WGTACW 1 cut(s) 790
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 3 cut(s) 135, 663, 872
Tru9I TTAA 3 cut(s) 135, 663, 872
TscAI CASTG 1 cut(s) 1030
TseFI GTSAC 2 cut(s) 611, 932
TseI GCWGC 5 cut(s) 7, 357, 508, 605, 639
Tsp45I GTSAC 2 cut(s) 611, 932
TspDTI ATGAA 2 cut(s) 607, 635
TspGWI ACGGA 2 cut(s) 237, 1330
TspRI CASTG 1 cut(s) 1030
VpaK11BI GGWCC 1 cut(s) 979
XbaI TCTAGA 1 cut(s) 1148
XceI RCATGY 3 cut(s) 373, 695, 1439
XcmI CCANNNNNNNNNTGG 1 cut(s) 812
XmiI GTMKAC 1 cut(s) 1057
XmnI GAANNNNTTC 1 cut(s) 673
XspI CTAG 9 cut(s) 288, 492, 975, 1011, 1137, 1149, 1179, 1191, 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.