RchiOBHm_Chr2g0136711

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012640
Physical Location & Seq
Forward (+)
54014598 .. 54017629
3032 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50749

Sequence Viewer

Length: 1308 bp
ATGCCCAAAGTCAGACGATCATCTTCCTTGTCTCTTGATCGATCTAGGGCATCACCTTATCCTTGCACCTCTGAGGATGCTGAGCAATACAAGTTGAATAGTCCGTTAGAAAGCACAGAGAACGCAAAAGAATGGGAAGATACTAGATGCCCCATCTGCATGGAACACCCACATAATGCTGTTCTTATAAAATGTTCCTCTCATGAGATGGGCTGCAGGCCGTACATGTGCAATACAAGCTACCGGCACTCAAATTGTCTTGACCAGTTTTGTAAGTCTTCTGTACCTGAGTCCACAACAATGATGTTGCAAGAACTTCCTCTGACACACACAGCCTCTCACGGTTCTAGGGAAGAAGAATCGCTGCCTGGGCAAACCAGCCCTTGTGGAAGTCAGTTGCAACCACATCCAGCTTGCCCTCTTTGTCGGGGAAAGATCTATGGGTATATTGTTGTAGAGGCTGCTCGTCACTTCATGAATTGCAAAGTGAGGAGCTGCTCTTCTGAGACATGCGACTTTAATGGAACTTATTCAGAACTGAGGAAGCATGCGAGGTCTGAACACCCTTATGTCCGGCCATCAGAGGTGGATCCAAATCGTCATCGTGATTGGATGAGATTGGAACGTCAAAGGGACTTGCAAGATGTACTTAGCATAGTCCAAGTAGGGTCTGGAGATGATATTATTCCGGATCCATCAACAGATGGTGGCTGGATGACTTCCCTTTTAAGTGCTATGTTTCGCTCGTTTGAGATTATGATTTTTTCTCAATTGATGGAGAGTTCAAGTGACATGGAACAAACTCATAGTAGAAGATCAGGGAGAATGACTAGGGTCCAGCAAGACAATGACACAATCCCTGCTGCTAGAAGGAACAACACTGTATCAGATAGCACATCCCGTCCCAGAGGTCTACGTTGGCGAGCACCAAATAATAATGGCGAGACAGCTCGTACCTCCAGATTGAGCCACAATTCTTTGTCAGGGGAGACTAGCCATACCTCTAGATGGAGCAACAACTCATTGGAGGAGGCTAGCCGTGCCCCTAGATGGGGTAACTCCTTATCTGAGGAGGGTACTAACCGTGTCCTTAGAAGGGGTAACAACAGCGTATCGGAGACTACCCGTCCCTCCAGATGGGGCAACAACTCTGTGCCGGAGGAGATAATCGTGTCCACAGATGGGGCAACAACTCCTTATCAGAGGAGAGTAGATGGGGCAACAACTCCTTATCGGAAGAGGCTGGGCATGCCTCTAGATGGGGCAATACTTCTGTACCAGAAAGCACACCCCGTCCTCGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

435

Amino Acids

49.1

Weight (kDa)

7.58

Isoelectric Point (pI)

65.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 46 - 218 3.2e-67 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AccI GTMKAC 1 cut(s) 913
AccIII TCCGGA 1 cut(s) 688
AclWI GGATC 4 cut(s) 584, 597, 686, 699
AcoI YGGCCR 1 cut(s) 575
AfaI GTAC 6 cut(s) 224, 285, 648, 955, 1078, 1277
AfiI CCNNNNNNNGG 8 cut(s) 1008, 1050, 1051, 1052, 1096, 1137, 1182, 1259
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 2 cut(s) 97, 786
AhdI GACNNNNNGTC 1 cut(s) 1125
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 4 cut(s) 240, 413, 495, 950
AluI AGCT 4 cut(s) 240, 413, 495, 950
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 5 cut(s) 36, 500, 938, 983, 1112
AlwI GGATC 4 cut(s) 584, 597, 686, 699
Aor13HI TCCGGA 1 cut(s) 688
AoxI GGCC 2 cut(s) 218, 575
ApeKI GCWGC 5 cut(s) 213, 364, 461, 495, 863
Asp700I GAANNNNTTC 1 cut(s) 529
AspS9I GGNCC 1 cut(s) 835
AsuHPI GGTGA 1 cut(s) 45
AsuNHI GCTAGC 1 cut(s) 1034
AvaII GGWCC 1 cut(s) 835
BaeGI GKGCMC 1 cut(s) 1045
BaeI ACNNNNGTAYC 2 cut(s) 1259, 1292
BamHI GGATCC 2 cut(s) 589, 691
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 5 cut(s) 200, 351, 448, 482, 850
BceAI ACGGC 2 cut(s) 205, 1023
BciT130I CCWGG 1 cut(s) 369
BcoDI GTCTC 5 cut(s) 36, 500, 938, 983, 1112
BfmI CTRYAG 1 cut(s) 214
BglII AGATCT 1 cut(s) 435
BisI GCNGC 5 cut(s) 214, 365, 462, 496, 864
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 5 cut(s) 215, 366, 463, 497, 865
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 835
BmeRI GACNNNNNGTC 1 cut(s) 1125
BmgT120I GGNCC 1 cut(s) 835
BmiI GGNNCC 3 cut(s) 591, 693, 836
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 3 cut(s) 59, 67, 137
BmtI GCTAGC 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 833
BpiI GAAGAC 1 cut(s) 270
BpmI CTGGAG 3 cut(s) 693, 943, 1117
Bpu1102I GCTNAGC 1 cut(s) 81
Bsa29I ATCGAT 1 cut(s) 40
BsaBI GATNNNNATC 1 cut(s) 594
BsaJI CCNNGG 1 cut(s) 368
BsaWI WCCGGW 1 cut(s) 688
Bsc4I CCNNNNNNNGG 8 cut(s) 1008, 1050, 1051, 1052, 1096, 1137, 1182, 1259
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 265
Bse8I GATNNNNATC 1 cut(s) 594
BseAI TCCGGA 1 cut(s) 688
BseBI CCWGG 1 cut(s) 369
BseCI ATCGAT 1 cut(s) 40
BseDI CCNNGG 1 cut(s) 368
BseGI GGATG 5 cut(s) 82, 406, 618, 720, 896
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 8 cut(s) 1008, 1050, 1051, 1052, 1096, 1137, 1182, 1259
BseMII CTCAG 6 cut(s) 63, 72, 279, 495, 530, 1059
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 5 cut(s) 505, 1043, 1085, 1175, 1219
BseSI GKGCMC 1 cut(s) 1045
BseXI GCAGC 5 cut(s) 200, 351, 448, 482, 850
BseYI CCCAGC 1 cut(s) 1243
BshFI GGCC 2 cut(s) 220, 577
BshVI ATCGAT 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 4 cut(s) 244, 574, 689, 1157
BslFI GGGAC 3 cut(s) 647, 888, 1113
BslI CCNNNNNNNGG 8 cut(s) 1008, 1050, 1051, 1052, 1096, 1137, 1182, 1259
BsmAI GTCTC 5 cut(s) 36, 500, 938, 983, 1112
BsmFI GGGAC 3 cut(s) 647, 888, 1113
BsnI GGCC 2 cut(s) 220, 577
Bsp1286I GDGCHC 2 cut(s) 928, 1045
Bsp13I TCCGGA 1 cut(s) 688
Bsp143I GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
Bsp1720I GCTNAGC 1 cut(s) 81
BspANI GGCC 2 cut(s) 220, 577
BspCNI CTCAG 6 cut(s) 64, 73, 280, 496, 531, 1060
BspDI ATCGAT 1 cut(s) 40
BspEI TCCGGA 1 cut(s) 688
BspHI TCATGA 2 cut(s) 202, 474
BspLI GGNNCC 3 cut(s) 591, 693, 836
BspMAI CTGCAG 1 cut(s) 218
BspOI GCTAGC 1 cut(s) 1038
BspPI GGATC 4 cut(s) 584, 597, 686, 699
BspQI GCTCTTC 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 344, 883, 1085
Bst6I CTCTTC 2 cut(s) 505, 1232
BstC8I GCNNGC 6 cut(s) 218, 415, 549, 924, 1036, 1250
BstDEI CTNAG 8 cut(s) 72, 81, 288, 504, 539, 650, 1068, 1091
BstF5I GGATG 5 cut(s) 82, 406, 618, 720, 896
BstKTI GATC 7 cut(s) 20, 40, 44, 438, 592, 694, 818
BstMAI GTCTC 5 cut(s) 36, 500, 938, 983, 1112
BstMBI GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
BstMWI GCNNNNNNNGC 5 cut(s) 156, 237, 370, 1040, 1249
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 4 cut(s) 229, 513, 551, 1252
BstPAI GACNNNNGTC 1 cut(s) 833
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 214
BstSLI GKGCMC 1 cut(s) 1045
BstV1I GCAGC 5 cut(s) 200, 351, 448, 482, 850
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 3 cut(s) 435, 589, 691
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 3 cut(s) 435, 589, 691
Bsu15I ATCGAT 1 cut(s) 40
BsuRI GGCC 2 cut(s) 220, 577
BsuTUI ATCGAT 1 cut(s) 40
BtsCI GGATG 5 cut(s) 82, 406, 618, 720, 896
BtsIMutI CAGTG 1 cut(s) 879
Cac8I GCNNGC 6 cut(s) 218, 415, 549, 924, 1036, 1250
CciI TCATGA 2 cut(s) 202, 474
Cfr10I RCCGGY 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 835
ClaI ATCGAT 1 cut(s) 40
Csp6I GTAC 6 cut(s) 223, 284, 647, 954, 1077, 1276
CviAII CATG 8 cut(s) 160, 203, 226, 475, 510, 548, 793, 1249
CviQI GTAC 6 cut(s) 223, 284, 647, 954, 1077, 1276
DdeI CTNAG 8 cut(s) 72, 81, 288, 504, 539, 650, 1068, 1091
DpnI GATC 7 cut(s) 19, 39, 43, 437, 591, 693, 817
DpnII GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
DriI GACNNNNNGTC 1 cut(s) 1125
EaeI YGGCCR 1 cut(s) 575
Eam1104I CTCTTC 2 cut(s) 505, 1232
Eam1105I GACNNNNNGTC 1 cut(s) 1125
EarI CTCTTC 2 cut(s) 505, 1232
Eco47I GGWCC 1 cut(s) 835
EcoRII CCWGG 1 cut(s) 367
FaeI CATG 8 cut(s) 163, 206, 229, 478, 513, 551, 796, 1252
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FaqI GGGAC 3 cut(s) 647, 888, 1113
FatI CATG 8 cut(s) 159, 202, 225, 474, 509, 547, 792, 1248
FblI GTMKAC 1 cut(s) 913
Fnu4HI GCNGC 5 cut(s) 214, 365, 462, 496, 864
FokI GGATG 5 cut(s) 89, 393, 625, 727, 883
Fsp4HI GCNGC 5 cut(s) 214, 365, 462, 496, 864
GluI GCNGC 5 cut(s) 214, 365, 462, 496, 864
GsaI CCCAGC 1 cut(s) 1247
GsuI CTGGAG 3 cut(s) 693, 943, 1117
HaeIII GGCC 2 cut(s) 220, 577
HapII CCGG 4 cut(s) 244, 574, 689, 1157
Hin1II CATG 8 cut(s) 163, 206, 229, 478, 513, 551, 796, 1252
HinfI GANTC 2 cut(s) 290, 359
HpaII CCGG 4 cut(s) 244, 574, 689, 1157
HphI GGTGA 1 cut(s) 45
Hpy166II GTNNAC 3 cut(s) 294, 914, 1176
Hpy8I GTNNAC 3 cut(s) 294, 914, 1176
HpyAV CCTTC 2 cut(s) 864, 1089
HpyCH4III ACNGT 3 cut(s) 344, 883, 1085
HpyCH4IV ACGT 2 cut(s) 625, 916
HpyCH4V TGCA 8 cut(s) 66, 159, 216, 231, 310, 400, 483, 640
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 237, 370, 1040, 1249
HpyF3I CTNAG 8 cut(s) 72, 81, 288, 504, 539, 650, 1068, 1091
HpySE526I ACGT 2 cut(s) 625, 916
Hsp92II CATG 8 cut(s) 163, 206, 229, 478, 513, 551, 796, 1252
Kpn2I TCCGGA 1 cut(s) 688
Kzo9I GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
LguI GCTCTTC 1 cut(s) 505
LmnI GCTCC 2 cut(s) 492, 1011
Lsp1109I GCAGC 5 cut(s) 200, 351, 448, 482, 850
LweI GCATC 3 cut(s) 59, 67, 137
MaeII ACGT 2 cut(s) 625, 916
MaeIII GTNAC 4 cut(s) 467, 788, 1055, 1100
MalI GATC 7 cut(s) 19, 39, 43, 437, 591, 693, 817
MboI GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
MboII GAAGA 8 cut(s) 15, 149, 270, 365, 368, 492, 825, 1249
MfeI CAATTG 1 cut(s) 770
MflI RGATCY 3 cut(s) 435, 589, 691
MhlI GDGCHC 2 cut(s) 928, 1045
MluCI AATT 4 cut(s) 253, 478, 770, 973
MlyI GAGTC 1 cut(s) 299
MroI TCCGGA 1 cut(s) 688
MroXI GAANNNNTTC 1 cut(s) 529
MseI TTAA 2 cut(s) 519, 728
MslI CAYNNNNRTG 3 cut(s) 158, 299, 567
MspI CCGG 4 cut(s) 244, 574, 689, 1157
MspR9I CCNGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 770
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 5 cut(s) 156, 237, 370, 1040, 1249
NdeII GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
NheI GCTAGC 1 cut(s) 1034
NlaIII CATG 8 cut(s) 163, 206, 229, 478, 513, 551, 796, 1252
NlaIV GGNNCC 3 cut(s) 591, 693, 836
NmuCI GTSAC 2 cut(s) 467, 788
NspI RCATGY 4 cut(s) 229, 513, 551, 1252
PaeI GCATGC 2 cut(s) 551, 1252
PagI TCATGA 2 cut(s) 202, 474
PciI ACATGT 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 505
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 1 cut(s) 359
PkrI GCNGC 5 cut(s) 215, 366, 463, 497, 865
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
PscI ACATGT 1 cut(s) 225
PshAI GACNNNNGTC 1 cut(s) 833
PsiI TTATAA 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 367
PspFI CCCAGC 1 cut(s) 1243
PspGI CCWGG 1 cut(s) 367
PspN4I GGNNCC 3 cut(s) 591, 693, 836
PspPI GGNCC 1 cut(s) 835
PstI CTGCAG 1 cut(s) 218
PsuI RGATCY 3 cut(s) 435, 589, 691
RsaI GTAC 6 cut(s) 224, 285, 648, 955, 1078, 1277
RsaNI GTAC 6 cut(s) 223, 284, 647, 954, 1077, 1276
RseI CAYNNNNRTG 3 cut(s) 158, 299, 567
SapI GCTCTTC 1 cut(s) 505
SaqAI TTAA 2 cut(s) 519, 728
SatI GCNGC 5 cut(s) 214, 365, 462, 496, 864
Sau3AI GATC 7 cut(s) 17, 37, 41, 435, 589, 691, 815
Sau96I GGNCC 1 cut(s) 835
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 2 cut(s) 928, 1045
SfaNI GCATC 3 cut(s) 59, 67, 137
SfcI CTRYAG 1 cut(s) 214
SinI GGWCC 1 cut(s) 835
SmiMI CAYNNNNRTG 3 cut(s) 158, 299, 567
SphI GCATGC 2 cut(s) 551, 1252
Sse9I AATT 4 cut(s) 253, 478, 770, 973
StyD4I CCNGG 1 cut(s) 367
TaaI ACNGT 3 cut(s) 344, 883, 1085
TaiI ACGT 2 cut(s) 628, 919
TaqI TCGA 2 cut(s) 40, 1299
TasI AATT 4 cut(s) 253, 478, 770, 973
TatI WGTACW 1 cut(s) 646
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 2 cut(s) 519, 728
Tru9I TTAA 2 cut(s) 519, 728
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 2 cut(s) 467, 788
TseI GCWGC 5 cut(s) 213, 364, 461, 495, 863
Tsp45I GTSAC 2 cut(s) 467, 788
TspDTI ATGAA 2 cut(s) 463, 491
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 1 cut(s) 886
VpaK11BI GGWCC 1 cut(s) 835
XbaI TCTAGA 2 cut(s) 1004, 1255
XceI RCATGY 4 cut(s) 229, 513, 551, 1252
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmiI GTMKAC 1 cut(s) 913
XmnI GAANNNNTTC 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.