Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0008635

Physical Location & Seq

Genomic Coordinates Forward (+)

23631143 .. 23637419

Gene Structure 6277 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g30510.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGCAATTATACCTTGTGGAAGTACATCGGTTGCTCAATGGGGCATTCGTCCTCAGCCTATGCTAAGATCCTATGCCATGAACAAGATACGCACATCCCAGTGTTGTGTTTCAAGCCAAATTGGCTCTTTGGGGGCACCAACATCAAGCGTCTCTCGGGATTCCTTGAATGCTCTATTCTGTGCAGGCCCATCTCACACTTCACATCGCAGTAGGGGAGCAAGATTCATAGTTAGAGCAGAGTCTGACTACTATTCTGTTCTTGGAGTGTCAAGAAATGCGACTAAAGCAGAAATTAAGAGCGCATACCGGAAGCTTGCAAGAAGTTATCATCCAGATGTGAACAAAGAAGCAGGGGCAGAAACAAAATTCAAGGAAATTAGTAATGCATATGAGGTCTTGTCTGATGATGAAAAACGATCCTTGTATGACAAATATGGAGAGGCTGGAGTTAAAGGTGCTGGTATGGGTCCAGGGGATTTCAGCAATCCATTTGACCTGTTTGAGTCACTGTTTGAGGGCATGGGGGGAATGGGATCCAGAGGTGGTGGTGGTTCTAGGAATAGAGCGGTTGATGGCCAGGACGAGTATTATAGTCTCGTCTTGAACTTCAAAGAAGCAGTTTTTGGGGTTGAAAAGGAAATTGAAATAAGTCGGTTGGAGAGCTGTGGAACTTGCAATGGTTCAGGGGCTAAAGCAGGAACCAAGCCATCCAGATGCAGCACATGTGGTGGGCAAGGTCAGGTTGTTCAATCAGCAAGGACTCCTTTGGGTGTCTTCCAGCAAGTAATGACATGCTCTTCCTGTGCCGGAACTGGGGAAATATCCACCCCTTGTAATACTTGCTCAGGTGATGGTCGTGTGAGGAAGACAAAGCGAATCAGTCTGAAAGTTCCAGCTGGTGTGGATGTAGGCAGCCGTTTAAGAGTTCGAAATGAAGGCAATGCTGGAAAACGAGGCGGGTCTCCGGGTGACTTATTTGTTATTATAGAAGTTATCCCAGACCCTGTCCTCAAACGTGATGATACCAACATTATGTACTCCTGCAAGGTGTCCTATATTGATGCAATTTTGGGGACTACAATTAAGGTTCCAACAGTTGATGGCATGGTTGATTTGAAGATTCCAGCAGGGACTCAACCAAACACGACCCTCGTGATGGCAAAGAAAGGTGTTCCCCTCTTAAACAAAAGCAACATGAGAGGTGATCAGCTGGTCAGAGTGCAGGTTGAAATCCCAAAGAGATTAAGCAATGATGAAAAGAAGCTCATTGAAGAACTCGCGGATCTGAGCAAGGGAAAGGCTGCAAGCAGGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

440

Amino Acids

46.97

Weight (kDa)

9.26

Isoelectric Point (pI)

38.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	83 - 144	1.7e-27	DnaJ domain
DnaJ_C	PF01556	198 - 413	8.2e-45	DnaJ C terminal domain
DnaJ_CXXCXGXG	PF00684	223 - 286	1.1e-14	DnaJ central domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22360 AT4G39960 AT4G39960
fragaria_vesca	FvH4_6g30510 FvH4_6g30510 FvH4_6g30510 FvH4_6g30510 FvH4_6g30510
malus_domestica	MD09G1219300.v1.1 MD17G1202800.v1.1
prunus_persica	Prupe.3G078100_v2.0.a1 Prupe.3G078100_v2.0.a1
pyrus_communis	pycom17g20670
rosa_chinensis	RchiOBHm_Chr2g0136911
rosa_laevigata	RLG00000019588
rosa_multiflora	Rmu_ssc0000071.1_g000001
rosa_roxburghii	Rroxscaffold_2G00107880
rosa_rugosa	Rorug02G0335200
rosa_samantha	Rh2AG385200 Rh2BG392300 Rh2CG372500 Rh2DG408500
rosa_wichuraiana	Rw2G031510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1216
AccB1I	GGYRCC	1 cut(s)	136
AccBSI	CCGCTC	1 cut(s)	569
AccII	CGCG	1 cut(s)	1283
AciI	CCGC	3 cut(s)	569, 960, 1283
AclWI	GGATC	5 cut(s)	63, 414, 531, 544, 1293
AcoI	YGGCCR	1 cut(s)	577
AcsI	RAATTY	1 cut(s)	368
AfaI	GTAC	2 cut(s)	25, 1040
AfiI	CCNNNNNNNGG	2 cut(s)	816, 1159
AflIII	ACRYGT	1 cut(s)	725
AjnI	CCWGG	2 cut(s)	472, 579
AjuI	GAANNNNNNNTTGG	2 cut(s)	609, 641
AluBI	AGCT	5 cut(s)	316, 666, 899, 1213, 1267
AluI	AGCT	5 cut(s)	316, 666, 899, 1213, 1267
Alw26I	GTCTC	3 cut(s)	157, 602, 969
AlwI	GGATC	5 cut(s)	63, 414, 531, 544, 1293
AlwNI	CAGNNNCTG	2 cut(s)	245, 1007
Ama87I	CYCGRG	1 cut(s)	156
AoxI	GGCC	2 cut(s)	187, 577
ApeKI	GCWGC	3 cut(s)	720, 915, 1304
ApoI	RAATTY	1 cut(s)	368
AspLEI	GCGC	1 cut(s)	305
AspS9I	GGNCC	2 cut(s)	188, 470
AsuC2I	CCSGG	1 cut(s)	969
AsuHPI	GGTGA	3 cut(s)	863, 983, 1217
AsuII	TTCGAA	1 cut(s)	931
AvaI	CYCGRG	1 cut(s)	156
AvaII	GGWCC	1 cut(s)	470
BaeGI	GKGCMC	1 cut(s)	139
BalI	TGGCCA	1 cut(s)	579
BamHI	GGATCC	1 cut(s)	536
BanI	GGYRCC	1 cut(s)	136
BauI	CACGAG	1 cut(s)	1154
BbsI	GAAGAC	2 cut(s)	769, 875
BbvCI	CCTCAGC	1 cut(s)	54
BbvI	GCAGC	3 cut(s)	732, 927, 1291
BccI	CCATC	6 cut(s)	199, 569, 718, 848, 1097, 1153
BceAI	ACGGC	1 cut(s)	903
BciT130I	CCWGG	2 cut(s)	474, 581
BclI	TGATCA	1 cut(s)	1207
BcnI	CCSGG	1 cut(s)	969
BcoDI	GTCTC	3 cut(s)	157, 602, 969
BfaI	CTAG	1 cut(s)	558
BfuAI	ACCTGC	1 cut(s)	1216
BglI	GCCNNNNNGGC	1 cut(s)	123
BisI	GCNGC	3 cut(s)	721, 916, 1305
BlsI	GCNGC	3 cut(s)	722, 917, 1306
Bme1390I	CCNGG	3 cut(s)	474, 581, 969
Bme18I	GGWCC	1 cut(s)	470
BmeT110I	CYCGRG	1 cut(s)	156
BmgT120I	GGNCC	2 cut(s)	188, 470
BmiI	GGNNCC	5 cut(s)	138, 471, 538, 703, 1092
BmrFI	CCNGG	3 cut(s)	474, 581, 969
BmrI	ACTGGG	2 cut(s)	94, 825
BmsI	GCATC	2 cut(s)	707, 1054
BmuI	ACTGGG	2 cut(s)	94, 825
BpiI	GAAGAC	2 cut(s)	769, 875
BpmI	CTGGAG	1 cut(s)	468
Bpu10I	CCTNAGC	2 cut(s)	54, 847
Bpu14I	TTCGAA	1 cut(s)	931
BpuMI	CCSGG	1 cut(s)	969
BsaI	GGTCTC	1 cut(s)	969
BsaJI	CCNNGG	1 cut(s)	473
BsaWI	WCCGGW	1 cut(s)	309
Bsc4I	CCNNNNNNNGG	2 cut(s)	816, 1159
Bse1I	ACTGG	2 cut(s)	100, 820
Bse3DI	GCAATG	3 cut(s)	685, 949, 1258
BseBI	CCWGG	2 cut(s)	474, 581
BseDI	CCNNGG	1 cut(s)	473
BseGI	GGATG	4 cut(s)	95, 331, 710, 913
BseLI	CCNNNNNNNGG	2 cut(s)	816, 1159
BseMI	GCAATG	3 cut(s)	685, 949, 1258
BseMII	CTCAG	3 cut(s)	68, 861, 1280
BseNI	ACTGG	2 cut(s)	100, 820
BseSI	GKGCMC	1 cut(s)	139
BseXI	GCAGC	3 cut(s)	732, 927, 1291
BsgI	GTGCAG	2 cut(s)	204, 1244
Bsh1236I	CGCG	1 cut(s)	1283
BshFI	GGCC	2 cut(s)	189, 579
BshNI	GGYRCC	1 cut(s)	136
BsiHKCI	CYCGRG	1 cut(s)	156
BsiSI	CCGG	3 cut(s)	310, 810, 968
BslFI	GGGAC	2 cut(s)	1090, 1147
BslI	CCNNNNNNNGG	2 cut(s)	816, 1159
BsmAI	GTCTC	3 cut(s)	157, 602, 969
BsmBI	CGTCTC	1 cut(s)	157
BsmFI	GGGAC	2 cut(s)	1090, 1147
BsmI	GAATGC	2 cut(s)	45, 175
BsnI	GGCC	2 cut(s)	189, 579
Bso31I	GGTCTC	1 cut(s)	969
BsoBI	CYCGRG	1 cut(s)	156
Bsp119I	TTCGAA	1 cut(s)	931
Bsp1286I	GDGCHC	1 cut(s)	139
Bsp143I	GATC	5 cut(s)	68, 419, 536, 1207, 1285
BspACI	CCGC	3 cut(s)	569, 960, 1283
BspANI	GGCC	2 cut(s)	189, 579
BspCNI	CTCAG	3 cut(s)	67, 860, 1281
BspFNI	CGCG	1 cut(s)	1283
BspLI	GGNNCC	5 cut(s)	138, 471, 538, 703, 1092
BspMI	ACCTGC	1 cut(s)	1216
BspPI	GGATC	5 cut(s)	63, 414, 531, 544, 1293
BspQI	GCTCTTC	1 cut(s)	805
BspT104I	TTCGAA	1 cut(s)	931
BspT107I	GGYRCC	1 cut(s)	136
BspTNI	GGTCTC	1 cut(s)	969
BsrBI	CCGCTC	1 cut(s)	569
BsrDI	GCAATG	3 cut(s)	685, 949, 1258
BsrI	ACTGG	2 cut(s)	100, 820
BssECI	CCNNGG	1 cut(s)	473
BssMI	GATC	5 cut(s)	68, 419, 536, 1207, 1285
BssSI	CACGAG	1 cut(s)	1154
Bst2BI	CACGAG	1 cut(s)	1154
Bst2UI	CCWGG	2 cut(s)	474, 581
Bst4CI	ACNGT	2 cut(s)	513, 1099
Bst6I	CTCTTC	1 cut(s)	805
BstBI	TTCGAA	1 cut(s)	931
BstC8I	GCNNGC	3 cut(s)	187, 318, 1309
BstDEI	CTNAG	4 cut(s)	54, 65, 847, 1289
BstF5I	GGATG	4 cut(s)	95, 331, 710, 913
BstFNI	CGCG	1 cut(s)	1283
BstHHI	GCGC	1 cut(s)	305
BstKTI	GATC	5 cut(s)	71, 422, 539, 1210, 1288
BstMAI	GTCTC	3 cut(s)	157, 602, 969
BstMBI	GATC	5 cut(s)	68, 419, 536, 1207, 1285
BstMWI	GCNNNNNNNGC	2 cut(s)	123, 287
BstNI	CCWGG	2 cut(s)	474, 581
BstNSI	RCATGY	2 cut(s)	729, 798
BstSCI	CCNGG	3 cut(s)	472, 579, 967
BstSLI	GKGCMC	1 cut(s)	139
BstUI	CGCG	1 cut(s)	1283
BstV1I	GCAGC	3 cut(s)	732, 927, 1291
BstV2I	GAAGAC	2 cut(s)	769, 875
BstX2I	RGATCY	3 cut(s)	68, 536, 1285
BstYI	RGATCY	3 cut(s)	68, 536, 1285
BsuRI	GGCC	2 cut(s)	189, 579
BtgZI	GCGATG	1 cut(s)	191
BtsCI	GGATG	4 cut(s)	95, 331, 710, 913
BtsIMutI	CAGTG	2 cut(s)	107, 509
BveI	ACCTGC	1 cut(s)	1216
Cac8I	GCNNGC	3 cut(s)	187, 318, 1309
CaiI	CAGNNNCTG	2 cut(s)	245, 1007
CfoI	GCGC	1 cut(s)	305
Cfr13I	GGNCC	2 cut(s)	188, 470
CseI	GACGC	1 cut(s)	139
Csp6I	GTAC	2 cut(s)	24, 1039
CviAII	CATG	6 cut(s)	79, 523, 726, 795, 1108, 1198
CviQI	GTAC	2 cut(s)	24, 1039
DdeI	CTNAG	4 cut(s)	54, 65, 847, 1289
DpnI	GATC	5 cut(s)	70, 421, 538, 1209, 1287
DpnII	GATC	5 cut(s)	68, 419, 536, 1207, 1285
EaeI	YGGCCR	1 cut(s)	577
Eam1104I	CTCTTC	1 cut(s)	805
EarI	CTCTTC	1 cut(s)	805
Eco31I	GGTCTC	1 cut(s)	969
Eco47I	GGWCC	1 cut(s)	470
Eco88I	CYCGRG	1 cut(s)	156
EcoRII	CCWGG	2 cut(s)	472, 579
EcoT22I	ATGCAT	1 cut(s)	391
Esp3I	CGTCTC	1 cut(s)	157
FaeI	CATG	6 cut(s)	82, 526, 729, 798, 1111, 1201
FalI	AAGNNNNNCTT	2 cut(s)	751, 783
FaqI	GGGAC	2 cut(s)	1090, 1147
FatI	CATG	6 cut(s)	78, 522, 725, 794, 1107, 1197
FauI	CCCGC	1 cut(s)	953
FauNDI	CATATG	1 cut(s)	391
FbaI	TGATCA	1 cut(s)	1207
Fnu4HI	GCNGC	3 cut(s)	721, 916, 1305
FokI	GGATG	4 cut(s)	82, 318, 697, 920
Fsp4HI	GCNGC	3 cut(s)	721, 916, 1305
FspBI	CTAG	1 cut(s)	558
GlaI	GCGC	1 cut(s)	304
GluI	GCNGC	3 cut(s)	721, 916, 1305
GsuI	CTGGAG	1 cut(s)	468
HaeIII	GGCC	2 cut(s)	189, 579
HapII	CCGG	3 cut(s)	310, 810, 968
HgaI	GACGC	1 cut(s)	139
HhaI	GCGC	1 cut(s)	305
Hin1II	CATG	6 cut(s)	82, 526, 729, 798, 1111, 1201
Hin6I	GCGC	1 cut(s)	303
HinP1I	GCGC	1 cut(s)	303
HindIII	AAGCTT	1 cut(s)	314
HinfI	GANTC	8 cut(s)	161, 225, 242, 506, 763, 879, 1123, 1135
HpaII	CCGG	3 cut(s)	310, 810, 968
HphI	GGTGA	3 cut(s)	863, 983, 1217
Hpy166II	GTNNAC	1 cut(s)	343
Hpy188I	TCNGA	5 cut(s)	247, 406, 888, 1220, 1290
Hpy188III	TCNNGA	7 cut(s)	158, 273, 335, 540, 604, 714, 1156
Hpy8I	GTNNAC	1 cut(s)	343
HpyAV	CCTTC	1 cut(s)	932
HpyCH4III	ACNGT	2 cut(s)	513, 1099
HpyCH4IV	ACGT	1 cut(s)	1018
HpyCH4V	TGCA	9 cut(s)	185, 320, 389, 678, 720, 1047, 1067, 1225, 1307
HpyF10VI	GCNNNNNNNGC	2 cut(s)	123, 287
HpyF3I	CTNAG	4 cut(s)	54, 65, 847, 1289
HpySE526I	ACGT	1 cut(s)	1018
Hsp92II	CATG	6 cut(s)	82, 526, 729, 798, 1111, 1201
HspAI	GCGC	1 cut(s)	303
Ksp22I	TGATCA	1 cut(s)	1207
Kzo9I	GATC	5 cut(s)	68, 419, 536, 1207, 1285
LguI	GCTCTTC	1 cut(s)	805
LmnI	GCTCC	1 cut(s)	218
Lsp1109I	GCAGC	3 cut(s)	732, 927, 1291
LweI	GCATC	2 cut(s)	707, 1054
MaeI	CTAG	1 cut(s)	558
MaeII	ACGT	1 cut(s)	1018
MaeIII	GTNAC	2 cut(s)	507, 971
MalI	GATC	5 cut(s)	70, 421, 538, 1209, 1287
MbiI	CCGCTC	1 cut(s)	569
MboI	GATC	5 cut(s)	68, 419, 536, 1207, 1285
MboII	GAAGA	5 cut(s)	769, 792, 880, 1132, 1286
MflI	RGATCY	3 cut(s)	68, 536, 1285
MhlI	GDGCHC	1 cut(s)	139
MlsI	TGGCCA	1 cut(s)	579
MluCI	AATT	8 cut(s)	6, 120, 294, 368, 378, 642, 1068, 1083
MluNI	TGGCCA	1 cut(s)	579
MlyI	GAGTC	4 cut(s)	251, 515, 757, 1129
MmeI	TCCRAC	2 cut(s)	639, 1118
Mox20I	TGGCCA	1 cut(s)	579
Mph1103I	ATGCAT	1 cut(s)	391
MscI	TGGCCA	1 cut(s)	579
MseI	TTAA	6 cut(s)	297, 453, 923, 1086, 1184, 1247
MslI	CAYNNNNRTG	3 cut(s)	100, 336, 715
Msp20I	TGGCCA	1 cut(s)	579
MspA1I	CMGCKG	2 cut(s)	899, 1213
MspI	CCGG	3 cut(s)	310, 810, 968
MspR9I	CCNGG	3 cut(s)	474, 581, 969
Mva1269I	GAATGC	2 cut(s)	45, 175
MvaI	CCWGG	2 cut(s)	474, 581
MvnI	CGCG	1 cut(s)	1283
MwoI	GCNNNNNNNGC	2 cut(s)	123, 287
NciI	CCSGG	1 cut(s)	969
NdeI	CATATG	1 cut(s)	391
NdeII	GATC	5 cut(s)	68, 419, 536, 1207, 1285
NlaIII	CATG	6 cut(s)	82, 526, 729, 798, 1111, 1201
NlaIV	GGNNCC	5 cut(s)	138, 471, 538, 703, 1092
NmuCI	GTSAC	2 cut(s)	507, 971
NsiI	ATGCAT	1 cut(s)	391
NspI	RCATGY	2 cut(s)	729, 798
NspV	TTCGAA	1 cut(s)	931
PciI	ACATGT	1 cut(s)	725
PciSI	GCTCTTC	1 cut(s)	805
PctI	GAATGC	2 cut(s)	45, 175
PfeI	GAWTC	4 cut(s)	161, 225, 879, 1123
PflFI	GACNNNGTC	1 cut(s)	1007
PkrI	GCNGC	3 cut(s)	722, 917, 1306
PleI	GAGTC	4 cut(s)	250, 514, 757, 1129
PpsI	GAGTC	4 cut(s)	250, 514, 757, 1129
PscI	ACATGT	1 cut(s)	725
Psp6I	CCWGG	2 cut(s)	472, 579
PspGI	CCWGG	2 cut(s)	472, 579
PspN4I	GGNNCC	5 cut(s)	138, 471, 538, 703, 1092
PspPI	GGNCC	2 cut(s)	188, 470
PstNI	CAGNNNCTG	2 cut(s)	245, 1007
PsuI	RGATCY	3 cut(s)	68, 536, 1285
PsyI	GACNNNGTC	1 cut(s)	1007
PvuII	CAGCTG	2 cut(s)	899, 1213
RsaI	GTAC	2 cut(s)	25, 1040
RsaNI	GTAC	2 cut(s)	24, 1039
RseI	CAYNNNNRTG	3 cut(s)	100, 336, 715
SapI	GCTCTTC	1 cut(s)	805
SaqAI	TTAA	6 cut(s)	297, 453, 923, 1086, 1184, 1247
SatI	GCNGC	3 cut(s)	721, 916, 1305
Sau3AI	GATC	5 cut(s)	68, 419, 536, 1207, 1285
Sau96I	GGNCC	2 cut(s)	188, 470
SchI	GAGTC	4 cut(s)	251, 515, 757, 1129
ScrFI	CCNGG	3 cut(s)	474, 581, 969
SduI	GDGCHC	1 cut(s)	139
SfaNI	GCATC	2 cut(s)	707, 1054
SfuI	TTCGAA	1 cut(s)	931
SinI	GGWCC	1 cut(s)	470
SmiMI	CAYNNNNRTG	3 cut(s)	100, 336, 715
Sse9I	AATT	8 cut(s)	6, 120, 294, 368, 378, 642, 1068, 1083
SsiI	CCGC	3 cut(s)	569, 960, 1283
SspMI	CTAG	1 cut(s)	558
StyD4I	CCNGG	3 cut(s)	472, 579, 967
TaaI	ACNGT	2 cut(s)	513, 1099
TaiI	ACGT	1 cut(s)	1021
TaqI	TCGA	1 cut(s)	931
TasI	AATT	8 cut(s)	6, 120, 294, 368, 378, 642, 1068, 1083
TatI	WGTACW	2 cut(s)	23, 1038
TfiI	GAWTC	4 cut(s)	161, 225, 879, 1123
Tru1I	TTAA	6 cut(s)	297, 453, 923, 1086, 1184, 1247
Tru9I	TTAA	6 cut(s)	297, 453, 923, 1086, 1184, 1247
TscAI	CASTG	2 cut(s)	107, 516
TseFI	GTSAC	2 cut(s)	507, 971
TseI	GCWGC	3 cut(s)	720, 915, 1304
Tsp45I	GTSAC	2 cut(s)	507, 971
TspDTI	ATGAA	5 cut(s)	95, 217, 426, 951, 1272
TspRI	CASTG	2 cut(s)	107, 516
Tth111I	GACNNNGTC	1 cut(s)	1007
VpaK11BI	GGWCC	1 cut(s)	470
XapI	RAATTY	1 cut(s)	368
XceI	RCATGY	2 cut(s)	729, 798
XspI	CTAG	1 cut(s)	558
Zsp2I	ATGCAT	1 cut(s)	391

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.