FvH4_6g30720

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009628
Physical Location & Seq
Forward (+)
23790710 .. 23794702
3993 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30720.t6

Sequence Viewer

Length: 3066 bp
ATGAAGATTGCATCTTTAGTTACTGTTTGGATTGTTTATGGGTTGTTGGGTATTGGGTTCACATATTCATACCCTACTTCAGCATTTGATGATCTCAGAAATGAAAGAAGTGAAACCACTGTCACTTATATCTATGATAGAATAGATGATGTGAATAAAGCATGTCAGTTTGTTCTATCTTCTGCTTCAGAACTGAAAGCTGAAGATGATAGGATTTATAGCATGAAGAAGCAGCTTTTTTTTGTTAATGGGGATTGGAGGCAGGAGGTGGGTAAGGATCCAATCATGCCATTTGATGATAGAGAAGTTCAAAGCGAGTATTTGGGTAACCGAACTCCATTGAACTTGGCTTCATTTTGGCTCGTGGATATTGATCGTGCCCATCGATCCAAGAAGTCACTAAGTGTTAGTGGCTTCATGGTTATGGGTATCACTATAGATGGTAGCTTTATGGATTATGGGTACCAAGGAACTCCGGAGTTCCGAATGTGGCGTAGCCATTCTCAAATGACAATTTCCTTCCAAGGGATTTACACCGAATCCAAGAAGAATGGTGGTGAAAGAGTGATGTGCTTATTGGGGAGTACAATGTTGCCGTCTCGGGAGCCTGATTCTGCTAATCCATGGGAGTGGCTGAAGGCATCTGATTCAAGTAACCAACCTCCTCTTTCTCAAGATGATCAGATTCTGCTTGTTCTTCACTTTCCGGTGACTTTCAACTTGACAAGTAGGGCTATCCGAGGGGAACTGAGAAGTTTAAATCCAAAGTCAAACTCGAAGTACTTTGATGAGGTTCACATTTTATCACAGTTGGGGAAGTCAGCAATGTATGAATTTGGTTCAGAAAAGATTGTTTCTAGAGCATGTGATCCATACCCTTATGATGATAGCTTGGTATATGGTGGCACTAGCAACTATAAAGGTCATACTATTTGCGAAATCCTTAAAGAAGTTGCACGGGATCAAGCTTTCACTGTTGTGCCAAACTGGAGATGCAATGGCACCGATGAGTTTTGCAGCAAGTTGGGGCCTTTTGTGACAGATAAAGAGATTAAGGAGTCAGATGGAAGCTTTAAAGGTGTTAAACTATATATGCAGGAAATCATGTGCGAACAAAAAGCTTCTGGTGGAAATGCCAGTTCTGCAAGGGTTTCGGCTGTATTTAGAGCTGTTTCTCCAATGGAGAATCTGTATACTGCAGCAAAGAGATCTGGGCTTAACAATATGACTGTTGCTGCTGAGGGAATTTGGAAATCTACCAGTGGACAGCTTTGCATGGTTGGTTGCCTTGGACTTGTTGACGTAGAAGGCAGCCGATGCAATACTAGGGTCTGTTTGTATGTACCTACATCTTTCTCCATAAAGCAACGAAGCATTCTTTATGGAAGTTTCTCTAGCATCAATAATACTGGTTCATCATATTTTCCTCTCTCGTTTGAAAAGCTAGTGCAGCCTTCAGAACTGTGGAATTATTTCAGAGTTTCCAGTCCAAACTACAAGTACACAAAAATCTCTTCAGCTGCTGTTGTCTTGGAAAAAAACGAGCCCTTCAGTGTTGGAACTGTGATCAAGAAATCTTTGCTGAGTTTCCCCAAACTGGAAGACACGGAAGCTTTTGAACTCAGTCTTTCTGTTCTCTCTGAGGATCTTACCCTTCATGTGTCTGCATTTCCTGATCCAATTCCGAAGCTTCAACCTCCAAAAGTAGACGTTCAGATGGAGATTCTCTCTGTGGGTCCCTTGTTTGGTCGCTATTGGTCTCCACAAAATGGTTCCACTGCACAAGAGGAGACTCCTTACCACACAAAGTCCGAATACACCGAAAAGCAGCTCCTTCTGAATGTATCAGCACAACTCACCATCACTGGGAAAGCCTATAGCAGTCTGTCTGTGCTATATTTGGAAGGCCTCTATGATCCACATGTTGGAAAGATGTATCTAGTTGGATGCCGGGATGTTCGAGCCTCGTGGAAGATCTTGTATGAGAGCATGGATCTTGAAGCCGGGCTGGATTGTTTGGTTGAAATGGTTGTGTCTTATCCTCCAACTACATCTCGATGGTTAGTCAATCCAGCAGCCCGTATTTCCATAGCCAGCCAAAGGACTGAAGATGACCCTCTTTACTTTAGTACAGTCAAGCTCCAAACTCTTCCAATTATGTACAGGAAGCAGCGGGAAGACATTCTCTCTCGTCGGGGGATTGAGGGAATCCTCCGAGTTTTGACTCTTTCACTAGCAATTTGTGGAATTTTAAGCCAACTGTTTTACATCAGGTATAATGTGGATTCTGTTCCTTACATGTCTCTAGTCATGCTAGGTATCCAAGCAATTGGGTATAGCATTCCTCTGGTCACAGGCGCTGAAGCACTCTTCAAGAAGTTAGCTACTGAGTCCTATGAAACAACAACGTATGGCCTCGATGACAGTCAGTGGTTTCGTATACTTGATTACACGGTTAAGCTTCTTCTGATGGCCTCATTGTTACTGACTTTAAGGCTTTGTCAGAAAGTGTGGAAATCTCGCATCAGGTTACTTGCACAAACTCCTCTTGAACCACATCGTGTCCCTAATGATAAACGGGTGCTTATGACCACCTCAGCAATACATCTGATTGGCTATGTAATGGTTCTAGTCGTTCATAGTATGAGGACCGGCCAAAGATCAATCCGCACAAAAAGCTATAAAATTGCCAGGGAAGACTCTCGTGGACTGTGGGAATGGGAAACGGAACTAGAGGAGTATGTGGGACTTGTCCAAGATTTTTTCCTACTCCCCCAGATGATTGGTAACCTGGTGTGGCAGATTGATTGTAAACCACTCAGAAAGCTTTATTTCATTGGGATCACACTGGTCAGACTCTTTCCTCACATTTATGATTATGTTAGAGCTCCGTCTCTCAACCCTTACTTTGCCGAGGAGTATGAGTTTGTCAACCCAGGCTTCTTCCCTCGGGATCCAGAATGTACGAGAGGTTGCCTTCCAGTTCCAAGGCATTTGAAGCTGAGCTTGTTTCTGGTGTCAATGAAAATGCTAGACAAGAGAACGACAAGGAGAATGATGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2921

Protein Analysis

1022

Amino Acids

115.94

Weight (kDa)

7.93

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 51 - 266 9.8e-34 DUF2921 lipocalin-like domain
DUF2921_N PF25333 285 - 476 4.8e-43 DUF2921 lipocalin-like domain
DUF2921_N PF25333 515 - 712 1.2e-36 DUF2921 lipocalin-like domain
DUF2921 PF11145 724 - 1012 2.7e-60 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 462
AccB1I GGYRCC 2 cut(s) 462, 1001
AccB7I CCANNNNNTGG 2 cut(s) 1769, 1925
AccI GTMKAC 3 cut(s) 1193, 1707, 2440
AccIII TCCGGA 1 cut(s) 475
AciI CCGC 2 cut(s) 2173, 2668
AcoI YGGCCR 1 cut(s) 2653
AcsI RAATTY 3 cut(s) 833, 1245, 2247
AcuI CTGAAG 9 cut(s) 63, 171, 222, 656, 1440, 1500, 1534, 2127, 2382
AdeI CACNNNGTG 2 cut(s) 404, 2561
AfaI GTAC 8 cut(s) 464, 586, 782, 1344, 1502, 2131, 2162, 2965
AfiI CCNNNNNNNGG 7 cut(s) 525, 1597, 1745, 1769, 1866, 1925, 2100
AflIII ACRYGT 2 cut(s) 1921, 2298
AjnI CCWGG 3 cut(s) 2690, 2790, 2935
AleI CACNNNNGTG 1 cut(s) 977
Alw21I GWGCWC 1 cut(s) 2890
Alw26I GTCTC 5 cut(s) 603, 1764, 1784, 2307, 2898
AlwNI CAGNNNCTG 3 cut(s) 688, 1523, 2360
Ama87I CYCGRG 2 cut(s) 600, 2949
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 5 cut(s) 1028, 1906, 2413, 2472, 2653
ApoI RAATTY 3 cut(s) 833, 1245, 2247
Asp700I GAANNNNTTC 2 cut(s) 1472, 2181
Asp718I GGTACC 1 cut(s) 462
AspLEI GCGC 1 cut(s) 2360
AspS9I GGNCC 3 cut(s) 1028, 1736, 2649
AsuC2I CCSGG 2 cut(s) 1952, 2005
AsuHPI GGTGA 3 cut(s) 569, 721, 1849
AvaI CYCGRG 2 cut(s) 600, 2949
AvaII GGWCC 2 cut(s) 1736, 2649
BaeGI GKGCMC 1 cut(s) 382
BamHI GGATCC 2 cut(s) 277, 2953
BanI GGYRCC 2 cut(s) 462, 1001
BanII GRGCYC 2 cut(s) 1548, 2890
BauI CACGAG 3 cut(s) 362, 1966, 2703
BbsI GAAGAC 3 cut(s) 1608, 2184, 2703
Bbv12I GWGCWC 1 cut(s) 2890
BbvCI CCTCAGC 2 cut(s) 1239, 2596
BccI CCATC 7 cut(s) 390, 434, 1058, 1711, 1868, 2052, 2464
BceAI ACGGC 1 cut(s) 580
BcgI CGANNNNNNTGC 2 cut(s) 1134, 1168
BciT130I CCWGG 3 cut(s) 2692, 2792, 2937
BciVI GTATCC 1 cut(s) 2330
BclI TGATCA 2 cut(s) 679, 1566
BcnI CCSGG 2 cut(s) 1952, 2005
BcoDI GTCTC 5 cut(s) 603, 1764, 1784, 2307, 2898
BfmI CTRYAG 3 cut(s) 435, 1197, 1876
BfoI RGCGCY 1 cut(s) 2361
BfuI GTATCC 1 cut(s) 2330
BglII AGATCT 2 cut(s) 1208, 1974
BlpI GCTNAGC 1 cut(s) 3002
BmcAI AGTACT 1 cut(s) 782
Bme1390I CCNGG 5 cut(s) 1952, 2005, 2692, 2792, 2937
Bme18I GGWCC 2 cut(s) 1736, 2649
BmeT110I CYCGRG 2 cut(s) 600, 2949
BmgT120I GGNCC 3 cut(s) 1028, 1736, 2649
BmiI GGNNCC 9 cut(s) 279, 464, 606, 1003, 1029, 1737, 1738, 1774, 2955
BmrFI CCNGG 5 cut(s) 1952, 2005, 2692, 2792, 2937
BmrI ACTGGG 1 cut(s) 1875
BmsI GCATC 7 cut(s) 20, 650, 983, 1307, 1407, 1937, 2532
BmuI ACTGGG 1 cut(s) 1875
BpiI GAAGAC 3 cut(s) 1608, 2184, 2703
BplI GAGNNNNNCTC 2 cut(s) 1712, 1744
BpmI CTGGAG 1 cut(s) 1009
Bpu10I CCTNAGC 2 cut(s) 1239, 2596
Bpu1102I GCTNAGC 1 cut(s) 3002
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 1952, 2005
Bsa29I ATCGAT 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 1764
BsaWI WCCGGW 2 cut(s) 475, 706
BsaXI ACNNNNNCTCC 4 cut(s) 1175, 1205, 2909, 2939
Bsc4I CCNNNNNNNGG 7 cut(s) 525, 1597, 1745, 1769, 1866, 1925, 2100
Bse118I RCCGGY 1 cut(s) 2651
Bse1I ACTGG 9 cut(s) 992, 1137, 1260, 1414, 1485, 1602, 1870, 2853, 2981
Bse3DI GCAATG 2 cut(s) 831, 1003
Bse8I GATNNNNATC 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 475
BseBI CCWGG 3 cut(s) 2692, 2792, 2937
BseCI ATCGAT 1 cut(s) 385
BseGI GGATG 2 cut(s) 1952, 1960
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 7 cut(s) 525, 1597, 1745, 1769, 1866, 1925, 2100
BseMI GCAATG 2 cut(s) 831, 1003
BseNI ACTGG 9 cut(s) 992, 1137, 1260, 1414, 1485, 1602, 1870, 2853, 2981
BseRI GAGGAG 5 cut(s) 654, 1802, 2535, 2750, 2930
BseSI GKGCMC 1 cut(s) 382
BsgI GTGCAG 2 cut(s) 1469, 1764
BshFI GGCC 5 cut(s) 1030, 1908, 2415, 2474, 2655
BshNI GGYRCC 2 cut(s) 462, 1001
BshVI ATCGAT 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 2890
BsiHKCI CYCGRG 2 cut(s) 600, 2949
BsiSI CCGG 5 cut(s) 476, 707, 1951, 2004, 2652
BslFI GGGAC 3 cut(s) 1722, 2549, 2760
BslI CCNNNNNNNGG 7 cut(s) 525, 1597, 1745, 1769, 1866, 1925, 2100
BsmAI GTCTC 5 cut(s) 603, 1764, 1784, 2307, 2898
BsmBI CGTCTC 2 cut(s) 603, 2898
BsmFI GGGAC 3 cut(s) 1722, 2549, 2760
BsmI GAATGC 2 cut(s) 1374, 2340
BsnI GGCC 5 cut(s) 1030, 1908, 2415, 2474, 2655
Bso31I GGTCTC 1 cut(s) 1764
BsoBI CYCGRG 2 cut(s) 600, 2949
Bsp1286I GDGCHC 3 cut(s) 382, 1548, 2890
Bsp13I TCCGGA 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 2160
Bsp1720I GCTNAGC 1 cut(s) 3002
Bsp19I CCATGG 1 cut(s) 623
BspACI CCGC 2 cut(s) 2173, 2668
BspANI GGCC 5 cut(s) 1030, 1908, 2415, 2474, 2655
BspDI ATCGAT 1 cut(s) 385
BspEI TCCGGA 1 cut(s) 475
BspLI GGNNCC 9 cut(s) 279, 464, 606, 1003, 1029, 1737, 1738, 1774, 2955
BspMAI CTGCAG 1 cut(s) 1201
BspT107I GGYRCC 2 cut(s) 462, 1001
BspTNI GGTCTC 1 cut(s) 1764
BsrDI GCAATG 2 cut(s) 831, 1003
BsrFI RCCGGY 1 cut(s) 2651
BsrGI TGTACA 1 cut(s) 2160
BsrI ACTGG 9 cut(s) 992, 1137, 1260, 1414, 1485, 1602, 1870, 2853, 2981
BssAI RCCGGY 1 cut(s) 2651
BssNAI GTATAC 2 cut(s) 1194, 2441
BssSI CACGAG 3 cut(s) 362, 1966, 2703
BssT1I CCWWGG 5 cut(s) 466, 523, 623, 1288, 2987
Bst1107I GTATAC 2 cut(s) 1194, 2441
Bst2BI CACGAG 3 cut(s) 362, 1966, 2703
Bst2UI CCWGG 3 cut(s) 2692, 2792, 2937
Bst6I CTCTTC 3 cut(s) 1519, 2154, 2375
BstAPI GCANNNNNTGC 1 cut(s) 1317
BstAUI TGTACA 1 cut(s) 2160
BstC8I GCNNGC 1 cut(s) 2095
BstDSI CCRYGG 1 cut(s) 623
BstEII GGTNACC 2 cut(s) 326, 2786
BstF5I GGATG 2 cut(s) 1952, 1960
BstH2I RGCGCY 1 cut(s) 2361
BstHHI GCGC 1 cut(s) 2360
BstMAI GTCTC 5 cut(s) 603, 1764, 1784, 2307, 2898
BstMWI GCNNNNNNNGC 5 cut(s) 1142, 1317, 1450, 2676, 2998
BstNI CCWGG 3 cut(s) 2692, 2792, 2937
BstNSI RCATGY 4 cut(s) 165, 867, 1925, 2302
BstPI GGTNACC 2 cut(s) 326, 2786
BstSCI CCNGG 5 cut(s) 1950, 2003, 2690, 2790, 2935
BstSFI CTRYAG 3 cut(s) 435, 1197, 1876
BstSLI GKGCMC 1 cut(s) 382
BstV2I GAAGAC 3 cut(s) 1608, 2184, 2703
BstX2I RGATCY 6 cut(s) 277, 1208, 1645, 1974, 1993, 2953
BstXI CCANNNNNNTGG 3 cut(s) 630, 2330, 2783
BstYI RGATCY 6 cut(s) 277, 1208, 1645, 1974, 1993, 2953
BstZ17I GTATAC 2 cut(s) 1194, 2441
Bsu15I ATCGAT 1 cut(s) 385
BsuI GTATCC 1 cut(s) 2330
BsuRI GGCC 5 cut(s) 1030, 1908, 2415, 2474, 2655
BsuTUI ATCGAT 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 623
BtsCI GGATG 2 cut(s) 1952, 1960
BtsI GCAGTG 1 cut(s) 1776
BtsIMutI CAGTG 8 cut(s) 117, 972, 1267, 1558, 1776, 1863, 2435, 2846
Cac8I GCNNGC 1 cut(s) 2095
CaiI CAGNNNCTG 3 cut(s) 688, 1523, 2360
CfoI GCGC 1 cut(s) 2360
Cfr10I RCCGGY 1 cut(s) 2651
Cfr13I GGNCC 3 cut(s) 1028, 1736, 2649
ClaI ATCGAT 1 cut(s) 385
CsiI ACCWGGT 1 cut(s) 2790
Csp6I GTAC 8 cut(s) 463, 585, 781, 1343, 1501, 2130, 2161, 2964
CviQI GTAC 8 cut(s) 463, 585, 781, 1343, 1501, 2130, 2161, 2964
DraI TTTAAA 2 cut(s) 759, 1075
DraIII CACNNNGTG 2 cut(s) 404, 2561
EaeI YGGCCR 1 cut(s) 2653
Eam1104I CTCTTC 3 cut(s) 1519, 2154, 2375
EarI CTCTTC 3 cut(s) 1519, 2154, 2375
Ecl136II GAGCTC 1 cut(s) 2888
Eco130I CCWWGG 5 cut(s) 466, 523, 623, 1288, 2987
Eco147I AGGCCT 1 cut(s) 1908
Eco24I GRGCYC 2 cut(s) 1548, 2890
Eco31I GGTCTC 1 cut(s) 1764
Eco47I GGWCC 2 cut(s) 1736, 2649
Eco53kI GAGCTC 1 cut(s) 2888
Eco57I CTGAAG 9 cut(s) 63, 171, 222, 656, 1440, 1500, 1534, 2127, 2382
Eco88I CYCGRG 2 cut(s) 600, 2949
Eco91I GGTNACC 2 cut(s) 326, 2786
EcoICRI GAGCTC 1 cut(s) 2888
EcoO109I RGGNCCY 2 cut(s) 1028, 1736
EcoO65I GGTNACC 2 cut(s) 326, 2786
EcoRII CCWGG 3 cut(s) 2690, 2790, 2935
EcoT14I CCWWGG 5 cut(s) 466, 523, 623, 1288, 2987
EcoT38I GRGCYC 2 cut(s) 1548, 2890
ErhI CCWWGG 5 cut(s) 466, 523, 623, 1288, 2987
Esp3I CGTCTC 2 cut(s) 603, 2898
FalI AAGNNNNNCTT 2 cut(s) 2990, 3022
FaqI GGGAC 3 cut(s) 1722, 2549, 2760
FauI CCCGC 1 cut(s) 2166
FbaI TGATCA 2 cut(s) 679, 1566
FblI GTMKAC 3 cut(s) 1193, 1707, 2440
FokI GGATG 2 cut(s) 1959, 1967
FriOI GRGCYC 2 cut(s) 1548, 2890
GlaI GCGC 1 cut(s) 2359
GsuI CTGGAG 1 cut(s) 1009
HaeII RGCGCY 1 cut(s) 2361
HaeIII GGCC 5 cut(s) 1030, 1908, 2415, 2474, 2655
HapII CCGG 5 cut(s) 476, 707, 1951, 2004, 2652
HhaI GCGC 1 cut(s) 2360
Hin6I GCGC 1 cut(s) 2358
HinP1I GCGC 1 cut(s) 2358
HincII GTYRAC 2 cut(s) 1300, 2932
HindII GTYRAC 2 cut(s) 1300, 2932
HindIII AAGCTT 7 cut(s) 966, 1069, 1119, 1611, 1688, 2459, 2825
HpaII CCGG 5 cut(s) 476, 707, 1951, 2004, 2652
HphI GGTGA 3 cut(s) 569, 721, 1849
Hpy99I CGWCG 1 cut(s) 2196
HpyAV CCTTC 9 cut(s) 529, 631, 1301, 1464, 1558, 1664, 1844, 1898, 2987
HpyCH4IV ACGT 3 cut(s) 1302, 1710, 2408
HpyF10VI GCNNNNNNNGC 5 cut(s) 1142, 1317, 1450, 2676, 2998
HpySE526I ACGT 3 cut(s) 1302, 1710, 2408
HspAI GCGC 1 cut(s) 2358
KflI GGGWCCC 1 cut(s) 1736
Kpn2I TCCGGA 1 cut(s) 475
KpnI GGTACC 1 cut(s) 466
Ksp22I TGATCA 2 cut(s) 679, 1566
LmnI GCTCC 4 cut(s) 604, 1836, 2145, 2893
LweI GCATC 7 cut(s) 20, 650, 983, 1307, 1407, 1937, 2532
MabI ACCWGGT 1 cut(s) 2790
MaeII ACGT 3 cut(s) 1302, 1710, 2408
MfeI CAATTG 1 cut(s) 2328
MflI RGATCY 6 cut(s) 277, 1208, 1645, 1974, 1993, 2953
MhlI GDGCHC 3 cut(s) 382, 1548, 2890
MlyI GAGTC 6 cut(s) 1067, 1786, 2218, 2399, 2693, 2850
MmeI TCCRAC 4 cut(s) 1537, 1906, 1924, 2069
MroI TCCGGA 1 cut(s) 475
MroXI GAANNNNTTC 2 cut(s) 1472, 2181
MslI CAYNNNNRTG 5 cut(s) 422, 628, 977, 2056, 2871
MspA1I CMGCKG 2 cut(s) 1520, 2173
MspI CCGG 5 cut(s) 476, 707, 1951, 2004, 2652
MspR9I CCNGG 5 cut(s) 1952, 2005, 2692, 2792, 2937
MunI CAATTG 1 cut(s) 2328
Mva1269I GAATGC 2 cut(s) 1374, 2340
MvaI CCWGG 3 cut(s) 2692, 2792, 2937
MwoI GCNNNNNNNGC 5 cut(s) 1142, 1317, 1450, 2676, 2998
NciI CCSGG 2 cut(s) 1952, 2005
NcoI CCATGG 1 cut(s) 623
NlaIV GGNNCC 9 cut(s) 279, 464, 606, 1003, 1029, 1737, 1738, 1774, 2955
NmeAIII GCCGAG 1 cut(s) 2938
NmuCI GTSAC 5 cut(s) 121, 396, 709, 1036, 2350
NspI RCATGY 4 cut(s) 165, 867, 1925, 2302
OliI CACNNNNGTG 1 cut(s) 977
PceI AGGCCT 1 cut(s) 1908
PciI ACATGT 2 cut(s) 1921, 2298
PcsI WCGNNNNNNNCGW 1 cut(s) 382
PctI GAATGC 2 cut(s) 1374, 2340
PdmI GAANNNNTTC 2 cut(s) 1472, 2181
PfeI GAWTC 8 cut(s) 539, 611, 647, 685, 1186, 1723, 2208, 2285
PflMI CCANNNNNTGG 2 cut(s) 1769, 1925
PleI GAGTC 6 cut(s) 1066, 1786, 2218, 2398, 2693, 2850
PpsI GAGTC 6 cut(s) 1066, 1786, 2218, 2398, 2693, 2850
PpuMI RGGWCCY 1 cut(s) 1736
PscI ACATGT 2 cut(s) 1921, 2298
Psp124BI GAGCTC 1 cut(s) 2890
Psp5II RGGWCCY 1 cut(s) 1736
Psp6I CCWGG 3 cut(s) 2690, 2790, 2935
PspEI GGTNACC 2 cut(s) 326, 2786
PspGI CCWGG 3 cut(s) 2690, 2790, 2935
PspN4I GGNNCC 9 cut(s) 279, 464, 606, 1003, 1029, 1737, 1738, 1774, 2955
PspPI GGNCC 3 cut(s) 1028, 1736, 2649
PspPPI RGGWCCY 1 cut(s) 1736
PstI CTGCAG 1 cut(s) 1201
PstNI CAGNNNCTG 3 cut(s) 688, 1523, 2360
PsuI RGATCY 6 cut(s) 277, 1208, 1645, 1974, 1993, 2953
PvuII CAGCTG 1 cut(s) 1520
RsaI GTAC 8 cut(s) 464, 586, 782, 1344, 1502, 2131, 2162, 2965
RsaNI GTAC 8 cut(s) 463, 585, 781, 1343, 1501, 2130, 2161, 2964
RseI CAYNNNNRTG 5 cut(s) 422, 628, 977, 2056, 2871
SacI GAGCTC 1 cut(s) 2890
Sau96I GGNCC 3 cut(s) 1028, 1736, 2649
ScaI AGTACT 1 cut(s) 782
SchI GAGTC 6 cut(s) 1067, 1786, 2218, 2399, 2693, 2850
ScrFI CCNGG 5 cut(s) 1952, 2005, 2692, 2792, 2937
SduI GDGCHC 3 cut(s) 382, 1548, 2890
SexAI ACCWGGT 1 cut(s) 2790
SfaNI GCATC 7 cut(s) 20, 650, 983, 1307, 1407, 1937, 2532
SfcI CTRYAG 3 cut(s) 435, 1197, 1876
SinI GGWCC 2 cut(s) 1736, 2649
SmiMI CAYNNNNRTG 5 cut(s) 422, 628, 977, 2056, 2871
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
SseBI AGGCCT 1 cut(s) 1908
SsiI CCGC 2 cut(s) 2173, 2668
SstI GAGCTC 1 cut(s) 2890
StuI AGGCCT 1 cut(s) 1908
StyD4I CCNGG 5 cut(s) 1950, 2003, 2690, 2790, 2935
StyI CCWWGG 5 cut(s) 466, 523, 623, 1288, 2987
TaiI ACGT 3 cut(s) 1305, 1713, 2411
TaqI TCGA 5 cut(s) 385, 776, 1960, 2056, 2418
TatI WGTACW 5 cut(s) 584, 780, 1500, 2129, 2160
TfiI GAWTC 8 cut(s) 539, 611, 647, 685, 1186, 1723, 2208, 2285
TscAI CASTG 8 cut(s) 124, 979, 1267, 1558, 1783, 1870, 2435, 2853
TseFI GTSAC 5 cut(s) 121, 396, 709, 1036, 2350
Tsp45I GTSAC 5 cut(s) 121, 396, 709, 1036, 2350
TspGWI ACGGA 3 cut(s) 1622, 2741, 2880
TspRI CASTG 8 cut(s) 124, 979, 1267, 1558, 1783, 1870, 2435, 2853
Van91I CCANNNNNTGG 2 cut(s) 1769, 1925
VpaK11BI GGWCC 2 cut(s) 1736, 2649
XapI RAATTY 3 cut(s) 833, 1245, 2247
XbaI TCTAGA 1 cut(s) 857
XceI RCATGY 4 cut(s) 165, 867, 1925, 2302
XmiI GTMKAC 3 cut(s) 1193, 1707, 2440
XmnI GAANNNNTTC 2 cut(s) 1472, 2181
ZrmI AGTACT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.