pycom17g20490

Protein of unknown function (DUF2921)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009628
Physical Location & Seq
Reverse (-)
18904404 .. 18907642
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g20490.1

Sequence Viewer

Length: 3195 bp
ATGATGAAGTTTGCATGTTTGGTTGTTGTGGTTTGGAGCGTTTATGGGTTGCTGTTTAATCTGGGGCTCACTTATTCAGAGCAATCAGAACCAGGATTCGAATTTACAAATGGAAGAAGTGGAAGCCCTGTTACTTACAGTTATGATCGGATCGATGAAGTGAAGAAAGAATGTCGTTTTGTTCTTTCTTCTGCTTCTGAACTGAAAGCTGAAGATAATCGAATTTATAGCATAAAAACTGAGCTTTTTTTTGTCAATGGGGACTGGAGGCAGGAGGTGGGTGATGCCCCAATTATCCCATTTGATGATAGAGAATTTGAAATGAGCTTGGTGGAGGATATTAATCGTACATCATCGAACTTGGTTTCATTTTGGGTTATGGATGTTGATCGTGCACTGATCGATCCAAGAAGTGGTAGCTTTGCGGATTATAGGTATGAAGGAAATCCAAAATTTCAAATATGGCCCGGCCATTCTCAGCTCACAGTTTCCTTCCAAGGGATTTACACTGAATCCAAGAAAAATGGTGGGGAAAGAGTAATGTGTTTGTTGGGGAGTACAATGTTGCCGTCCCGGGAGACTGATTCTGCTAATCCCTGGGAGTGGTTGAAGGCTTCTGATGTAAATTATGACCAACCTCCTCTTTCTGAAGATGATCAGATTCTACTAGTTCTTCATTATCCAGCGACTTTCTCATTGACAAATAGGGTTATCCAAGGAGAATTAAGAAGCTTAAATTCAAAGTCAAACTCAAAGTACTTTGATACGGTTCACATTTCATCGCAGTTGGGAAAGTCCGCAACGTATGAGTTTGGAGCTGAAAAGATTGTGTCAAGAGCATGTGATCCGTACCCTTCTAATGATAACTTGACATATGGTGGCATTAGCATTTACAAAGGTCCTTCGTTTTGTGAAATCCTCCAAGAAGTTACACGGGAACAAGCTTTCACTGTCTTGCCAAACTGGAGATGTAATTTCCCGGGTGATTTTTGCAGCAAGCTGGGTCCATTTGTGGCAGATAAAGAGATTAGGGCATCAAATGGAAGCTTTAAAGGTGTGAAAATTTTTATGCAGGATATCAAGTGTGAACAAAAAAATTCCGCAGTAAATGCCAGTTCAGCAAGGGTTTCAGCTGTGTTTAGGGCTGTTTCCCCGCTAGAGAATGACTATACTGCGGCAAAGAGATCTGGGCTTAACAACATGACCGTTGCAGCAGAGGGAATTTGGAAGTCTACCAGCGGGCAGCTTTGCATGGCTGGTTGCCTTGGACTTGCTGATGTTCAAGGACGTCAATGCAATAGTCGAATCTGCCTGTACATACCAGTCTCTTTTTCCATAAAGCAACGAAGCATTATTTATGGTAGTTTATCCAGCATCAATAACAGTGGTGCATCGTTTTTTCCTCTCTCCTTTGAAAAGCTAGTGCAGCCTACAGAGCTGTGGAATTACTTCAGAACCTCCAGTCCAAACTACAGGTACACAAAACTTGATTCAGCTGCTATTATCCTGGAAAAAAATGAGGCCTTTAGTGTTGGAACCATCATAAAAAAATCACTGCTGAATTTTCCCAAACTGGAAGATACAGAATCTTTTCAAGTCAGTCTTTCTCTTCTCTCGGAAGACCTAACCCTTCACGAGTCTGCGTTTCTCGACCCAATCCGAGATCTTTACTCATCAAGAATAGATATTCAGATGGAGATTCTGTCAGTTGGTCCCTTGTTTGGGCGCTTTTGGTCTCCACAAAACAGTTCCAGTGCAGAAGAGGAGACTCCTTACCACACGAAGGCTGAATACACTGAAAAGCAGCTCCTTATGAATGTATCCGCACAACTCACCATCACTGGGAAAGGTTTCAGCAATTTTTCAGTTCTCTTTCTGGAGGGTCTTTATGACCCACATGTGGGAAAGATGTATCTAGTTGGCTGCAGGGATGTTCGAGCCTCATGGAAGATCTTGTATGAGAGCATGGATCTTGAGGCTGGGCTTGATTGTTTAATTGAGGTGGTTGTGTCATATCCTCCCACTACATCTCTATGGTTAGGCAATCCAACAGCAAGTATTTCCGTAGCCAGTCAAAGGAATGAAGATGACCCCCTTTTCTTTAGTACAGTCAAGCTCAGAACTGTTCCTATTATGTACAGAAAACAGCGGGAAAGCATACTCTCTCGTCGGGGTATTGAGGGAATCCTCCGGATTTTGACTCTTTCCCTGGCAATTTCTGGCATTTTAAGCCAACTGTTTTACATCAGGCATAATGTGGATTTTGTTCCTTACATCTCTCTGGTCATGCTAGGTATCCAAGCCATTGGGTACAGCATTCCTCTGGTCACTGACGCTGAAGCGCTCTTCAAGAGAATATCTTCTGACTCCAATGCAATATCTTCGTATGACCTTGAGAACAATCAGTGGTACCATATACTTGATTACACAGTAAAGTTTCTGGTGATGGTTTCACTTTTACTGACTTTAAGGCTCTGTCAGAAGGTGTGGAAATCTCGAATCAGATTACTTACGCAAACTCCTCTTGAGCCACATCGTGTACCGAGTGATAAACGGGTGCTTCTGACTACCTTTGCAATACATTTTATTGGCTATGTCATTGTCTTAATCATTCATAGTATGACGACTAGCAGAAGATATATTCGGACAAAAAGCTATAGAATAGCTAGAGCAAATTCTCATGCACTGTGGGAGTGGGAAACTGAGCTAGAGGAGTATGTCGGTCTTGTTCAAGATTTTTTCCTTATCCCACAAATCATTGGCAACGTTGTGTGGCAGATTGATTGCAAACCGCTCAGAAAGTTCTATTTCTTTGCAATCACATTGGTCAGAATCTTCCCTCACATTTATGATTATATTAGAGCTCCTGCTCTCAATCCTTACTTTGCAGAGGACTACGAGCTTGTCAACCCGACTACGGATTTTTACTCCAAGTTTGGGGATATTGCCATTCCGGTTACAGCAATCATCCTTGCAGGTATAGTCTATGCTCAGCAGAGGTGGAGTTACGAGAGGATCAGCCAGACTCTTACTGTGGGGCAATATAGGCTTCTTCCTTTGGGTTCGAGAATGTATGAGAGGTTGCCTTCAAGTTCCATGGCATTTGAAGCTGAGCTTGTTTCCGGTGTCAATGGTAATGCTAGACACGAGAAAGAGCAAGAGAATGATGATGGAGAGTTGAATCGTAATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2921

Protein Analysis

1065

Amino Acids

120.7

Weight (kDa)

5.81

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 54 - 258 7.9e-32 DUF2921 lipocalin-like domain
DUF2921_N PF25333 277 - 469 5.9e-42 DUF2921 lipocalin-like domain
DUF2921_N PF25333 509 - 704 9.5e-33 DUF2921 lipocalin-like domain
DUF2921 PF11145 716 - 1003 1.3e-57 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1291
Acc36I ACCTGC 1 cut(s) 2966
Acc65I GGTACC 1 cut(s) 2409
AccB1I GGYRCC 1 cut(s) 2409
AccB7I CCANNNNNTGG 1 cut(s) 413
AccBSI CCGCTC 1 cut(s) 2794
AccI GTMKAC 1 cut(s) 1232
AccIII TCCGGA 1 cut(s) 2190
AciI CCGC 9 cut(s) 425, 798, 1101, 1154, 1175, 1239, 1824, 2149, 2792
AclI AACGTT 1 cut(s) 2766
AclWI GGATC 5 cut(s) 158, 398, 839, 1977, 3023
AcoI YGGCCR 1 cut(s) 469
AcuI CTGAAG 4 cut(s) 231, 669, 1435, 2358
AcyI GRCGYC 1 cut(s) 1288
AdeI CACNNNGTG 1 cut(s) 2537
AfeI AGCGCT 1 cut(s) 2343
AflIII ACRYGT 1 cut(s) 1897
AhlI ACTAGT 1 cut(s) 667
AjnI CCWGG 4 cut(s) 91, 596, 1506, 2208
AloI GAACNNNNNNTCC 2 cut(s) 1447, 1479
Alw21I GWGCWC 2 cut(s) 397, 2866
Alw26I GTCTC 4 cut(s) 572, 1330, 1740, 1760
Alw44I GTGCAC 1 cut(s) 393
AlwI GGATC 5 cut(s) 158, 398, 839, 1977, 3023
Ama87I CYCGRG 2 cut(s) 573, 979
Aor13HI TCCGGA 1 cut(s) 2190
Aor51HI AGCGCT 1 cut(s) 2343
AoxI GGCC 3 cut(s) 464, 469, 1521
ApaLI GTGCAC 1 cut(s) 393
ApeKI GCWGC 7 cut(s) 993, 1211, 1243, 1426, 1496, 1804, 1923
AseI ATTAAT 1 cut(s) 342
Asp700I GAANNNNTTC 4 cut(s) 1448, 1590, 1850, 2359
Asp718I GGTACC 1 cut(s) 2409
AspLEI GCGC 2 cut(s) 1728, 2344
AspS9I GGNCC 4 cut(s) 465, 899, 1004, 1712
AsuC2I CCSGG 5 cut(s) 468, 574, 575, 980, 981
AsuHPI GGTGA 4 cut(s) 293, 995, 1825, 2455
AsuII TTCGAA 1 cut(s) 99
AvaI CYCGRG 2 cut(s) 573, 979
AvaII GGWCC 3 cut(s) 899, 1004, 1712
BaeGI GKGCMC 1 cut(s) 397
BanI GGYRCC 1 cut(s) 2409
BanII GRGCYC 2 cut(s) 69, 2866
BarI GAAGNNNNNNTAC 4 cut(s) 115, 147, 1775, 1807
BauI CACGAG 2 cut(s) 1634, 3146
BbsI GAAGAC 1 cut(s) 1626
Bbv12I GWGCWC 2 cut(s) 397, 2866
BbvI GCAGC 7 cut(s) 1005, 1223, 1255, 1438, 1483, 1816, 1910
BccI CCATC 5 cut(s) 1547, 1687, 1844, 2440, 3164
BceAI ACGGC 1 cut(s) 553
BcgI CGANNNNNNTGC 2 cut(s) 2364, 2398
BciT130I CCWGG 4 cut(s) 93, 598, 1508, 2210
BciVI GTATCC 2 cut(s) 1831, 2306
BclI TGATCA 1 cut(s) 655
BcnI CCSGG 5 cut(s) 468, 574, 575, 980, 981
BcoDI GTCTC 4 cut(s) 572, 1330, 1740, 1760
BcuI ACTAGT 1 cut(s) 667
BfaI CTAG 9 cut(s) 668, 1157, 1421, 1916, 2291, 2628, 2667, 2708, 3141
BfmI CTRYAG 4 cut(s) 1431, 1471, 1924, 2656
BfoI RGCGCY 2 cut(s) 1729, 2345
BfuAI ACCTGC 1 cut(s) 2966
BfuI GTATCC 2 cut(s) 1831, 2306
BglII AGATCT 3 cut(s) 1184, 1663, 1950
BisI GCNGC 8 cut(s) 994, 1176, 1212, 1244, 1427, 1497, 1805, 1924
BlpI GCTNAGC 2 cut(s) 2991, 3111
BlsI GCNGC 8 cut(s) 995, 1177, 1213, 1245, 1428, 1498, 1806, 1925
BmcAI AGTACT 1 cut(s) 758
Bme1390I CCNGG 9 cut(s) 93, 468, 574, 575, 598, 980, 981, 1508, 2210
Bme18I GGWCC 3 cut(s) 899, 1004, 1712
BmeT110I CYCGRG 2 cut(s) 573, 979
BmgT120I GGNCC 4 cut(s) 465, 899, 1004, 1712
BmiI GGNNCC 4 cut(s) 1005, 1537, 1714, 2411
BmrFI CCNGG 9 cut(s) 93, 468, 574, 575, 598, 980, 981, 1508, 2210
BmrI ACTGGG 1 cut(s) 1851
BmsI GCATC 4 cut(s) 274, 1043, 1383, 1400
BmuI ACTGGG 1 cut(s) 1851
BpiI GAAGAC 1 cut(s) 1626
BpmI CTGGAG 4 cut(s) 286, 985, 1444, 1898
Bpu1102I GCTNAGC 2 cut(s) 2991, 3111
Bpu14I TTCGAA 1 cut(s) 99
BpuEI CTTGAG 3 cut(s) 1994, 2414, 2546
BpuMI CCSGG 5 cut(s) 468, 574, 575, 980, 981
Bsa29I ATCGAT 2 cut(s) 153, 402
BsaBI GATNNNNATC 2 cut(s) 342, 387
BsaHI GRCGYC 1 cut(s) 1288
BsaI GGTCTC 1 cut(s) 1740
BsaJI CCNNGG 9 cut(s) 496, 573, 596, 597, 715, 979, 1264, 2208, 3096
BsaWI WCCGGW 3 cut(s) 2190, 2953, 3122
BsaXI ACNNNNNCTCC 4 cut(s) 1688, 1718, 2503, 2533
Bse1I ACTGG 9 cut(s) 269, 968, 1113, 1322, 1461, 1578, 1752, 1846, 2070
Bse8I GATNNNNATC 2 cut(s) 342, 387
BseAI TCCGGA 1 cut(s) 2190
BseBI CCWGG 4 cut(s) 93, 598, 1508, 2210
BseCI ATCGAT 2 cut(s) 153, 402
BseDI CCNNGG 9 cut(s) 496, 573, 596, 597, 715, 979, 1264, 2208, 3096
BseGI GGATG 3 cut(s) 388, 1936, 2967
BseJI GATNNNNATC 2 cut(s) 342, 387
BseMII CTCAG 7 cut(s) 231, 491, 2131, 2694, 2809, 3005, 3102
BseNI ACTGG 9 cut(s) 269, 968, 1113, 1322, 1461, 1578, 1752, 1846, 2070
BseRI GAGGAG 4 cut(s) 630, 1778, 2511, 2726
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 7 cut(s) 1005, 1223, 1255, 1438, 1483, 1816, 1910
BseYI CCCAGC 2 cut(s) 1000, 1979
BsgI GTGCAG 2 cut(s) 1445, 1776
BshFI GGCC 3 cut(s) 466, 471, 1523
BshNI GGYRCC 1 cut(s) 2409
BshVI ATCGAT 2 cut(s) 153, 402
BsiHKAI GWGCWC 2 cut(s) 397, 2866
BsiHKCI CYCGRG 2 cut(s) 573, 979
BsiSI CCGG 6 cut(s) 468, 574, 980, 2191, 2954, 3123
BslFI GGGAC 3 cut(s) 275, 556, 1698
BsmAI GTCTC 4 cut(s) 572, 1330, 1740, 1760
BsmFI GGGAC 3 cut(s) 275, 556, 1698
BsmI GAATGC 1 cut(s) 2316
BsnI GGCC 3 cut(s) 466, 471, 1523
Bso31I GGTCTC 1 cut(s) 1740
BsoBI CYCGRG 2 cut(s) 573, 979
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 3 cut(s) 69, 397, 2866
Bsp13I TCCGGA 1 cut(s) 2190
Bsp1407I TGTACA 2 cut(s) 1314, 2136
Bsp1720I GCTNAGC 2 cut(s) 2991, 3111
Bsp19I CCATGG 1 cut(s) 3096
BspACI CCGC 9 cut(s) 425, 798, 1101, 1154, 1175, 1239, 1824, 2149, 2792
BspANI GGCC 3 cut(s) 466, 471, 1523
BspCNI CTCAG 7 cut(s) 232, 490, 2130, 2695, 2808, 3004, 3103
BspDI ATCGAT 2 cut(s) 153, 402
BspEI TCCGGA 1 cut(s) 2190
BspLI GGNNCC 4 cut(s) 1005, 1537, 1714, 2411
BspMAI CTGCAG 1 cut(s) 1928
BspMI ACCTGC 1 cut(s) 2966
BspPI GGATC 5 cut(s) 158, 398, 839, 1977, 3023
BspQI GCTCTTC 1 cut(s) 2351
BspT104I TTCGAA 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 2409
BspTNI GGTCTC 1 cut(s) 1740
BsrBI CCGCTC 1 cut(s) 2794
BsrGI TGTACA 2 cut(s) 1314, 2136
BsrI ACTGG 9 cut(s) 269, 968, 1113, 1322, 1461, 1578, 1752, 1846, 2070
BssECI CCNNGG 9 cut(s) 496, 573, 596, 597, 715, 979, 1264, 2208, 3096
BssNI GRCGYC 1 cut(s) 1288
BssSI CACGAG 2 cut(s) 1634, 3146
BssT1I CCWWGG 4 cut(s) 496, 715, 1264, 3096
Bst2BI CACGAG 2 cut(s) 1634, 3146
Bst2UI CCWGG 4 cut(s) 93, 598, 1508, 2210
Bst6I CTCTTC 3 cut(s) 1614, 1755, 2351
BstACI GRCGYC 1 cut(s) 1288
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstAUI TGTACA 2 cut(s) 1314, 2136
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 998, 1241
BstDEI CTNAG 7 cut(s) 240, 477, 2117, 2703, 2795, 2991, 3111
BstDSI CCRYGG 1 cut(s) 3096
BstF5I GGATG 3 cut(s) 388, 1936, 2967
BstH2I RGCGCY 2 cut(s) 1729, 2345
BstHHI GCGC 2 cut(s) 1728, 2344
BstMAI GTCTC 4 cut(s) 572, 1330, 1740, 1760
BstMWI GCNNNNNNNGC 7 cut(s) 1109, 1118, 1426, 1435, 2229, 3046, 3107
BstNI CCWGG 4 cut(s) 93, 598, 1508, 2210
BstNSI RCATGY 3 cut(s) 18, 843, 1901
BstSCI CCNGG 9 cut(s) 91, 466, 572, 573, 596, 978, 979, 1506, 2208
BstSFI CTRYAG 4 cut(s) 1431, 1471, 1924, 2656
BstSLI GKGCMC 1 cut(s) 397
BstV1I GCAGC 7 cut(s) 1005, 1223, 1255, 1438, 1483, 1816, 1910
BstV2I GAAGAC 1 cut(s) 1626
BstX2I RGATCY 4 cut(s) 1184, 1663, 1950, 1969
BstXI CCANNNNNNTGG 1 cut(s) 2306
BstYI RGATCY 4 cut(s) 1184, 1663, 1950, 1969
Bsu15I ATCGAT 2 cut(s) 153, 402
BsuI GTATCC 2 cut(s) 1831, 2306
BsuRI GGCC 3 cut(s) 466, 471, 1523
BsuTUI ATCGAT 2 cut(s) 153, 402
BtgI CCRYGG 1 cut(s) 3096
BtgZI GCGATG 1 cut(s) 765
BtsCI GGATG 3 cut(s) 388, 1936, 2967
BtsI GCAGTG 1 cut(s) 1553
BveI ACCTGC 1 cut(s) 2966
Cac8I GCNNGC 2 cut(s) 998, 1241
CfoI GCGC 2 cut(s) 1728, 2344
Cfr13I GGNCC 4 cut(s) 465, 899, 1004, 1712
Cfr9I CCCGGG 2 cut(s) 573, 979
ClaI ATCGAT 2 cut(s) 153, 402
CseI GACGC 1 cut(s) 2342
CspCI CAANNNNNGTGG 2 cut(s) 1366, 1401
DdeI CTNAG 7 cut(s) 240, 477, 2117, 2703, 2795, 2991, 3111
DraI TTTAAA 1 cut(s) 1051
DraIII CACNNNGTG 1 cut(s) 2537
EaeI YGGCCR 1 cut(s) 469
Eam1104I CTCTTC 3 cut(s) 1614, 1755, 2351
EarI CTCTTC 3 cut(s) 1614, 1755, 2351
Ecl136II GAGCTC 1 cut(s) 2864
Eco130I CCWWGG 4 cut(s) 496, 715, 1264, 3096
Eco147I AGGCCT 1 cut(s) 1523
Eco24I GRGCYC 2 cut(s) 69, 2866
Eco31I GGTCTC 1 cut(s) 1740
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 3 cut(s) 899, 1004, 1712
Eco47III AGCGCT 1 cut(s) 2343
Eco53kI GAGCTC 1 cut(s) 2864
Eco57I CTGAAG 4 cut(s) 231, 669, 1435, 2358
Eco88I CYCGRG 2 cut(s) 573, 979
EcoICRI GAGCTC 1 cut(s) 2864
EcoO109I RGGNCCY 1 cut(s) 899
EcoRII CCWGG 4 cut(s) 91, 596, 1506, 2208
EcoRV GATATC 1 cut(s) 1078
EcoT14I CCWWGG 4 cut(s) 496, 715, 1264, 3096
EcoT38I GRGCYC 2 cut(s) 69, 2866
ErhI CCWWGG 4 cut(s) 496, 715, 1264, 3096
FalI AAGNNNNNCTT 4 cut(s) 1587, 1619, 3099, 3131
FaqI GGGAC 3 cut(s) 275, 556, 1698
FauI CCCGC 3 cut(s) 1161, 1232, 2142
FauNDI CATATG 1 cut(s) 874
FbaI TGATCA 1 cut(s) 655
FblI GTMKAC 1 cut(s) 1232
Fnu4HI GCNGC 8 cut(s) 994, 1176, 1212, 1244, 1427, 1497, 1805, 1924
FokI GGATG 3 cut(s) 395, 1943, 2954
FriOI GRGCYC 2 cut(s) 69, 2866
Fsp4HI GCNGC 8 cut(s) 994, 1176, 1212, 1244, 1427, 1497, 1805, 1924
FspBI CTAG 9 cut(s) 668, 1157, 1421, 1916, 2291, 2628, 2667, 2708, 3141
GlaI GCGC 2 cut(s) 1727, 2343
GluI GCNGC 8 cut(s) 994, 1176, 1212, 1244, 1427, 1497, 1805, 1924
GsaI CCCAGC 2 cut(s) 1004, 1983
GsuI CTGGAG 4 cut(s) 286, 985, 1444, 1898
HaeII RGCGCY 2 cut(s) 1729, 2345
HaeIII GGCC 3 cut(s) 466, 471, 1523
HapII CCGG 6 cut(s) 468, 574, 980, 2191, 2954, 3123
HgaI GACGC 1 cut(s) 2342
HhaI GCGC 2 cut(s) 1728, 2344
Hin1I GRCGYC 1 cut(s) 1288
Hin6I GCGC 2 cut(s) 1726, 2342
HinP1I GCGC 2 cut(s) 1726, 2342
HincII GTYRAC 1 cut(s) 2908
HindII GTYRAC 1 cut(s) 2908
HindIII AAGCTT 3 cut(s) 730, 942, 1045
HpaII CCGG 6 cut(s) 468, 574, 980, 2191, 2954, 3123
HphI GGTGA 4 cut(s) 293, 995, 1825, 2455
Hpy166II GTNNAC 7 cut(s) 395, 772, 1088, 1233, 1479, 2540, 2908
Hpy8I GTNNAC 7 cut(s) 395, 772, 1088, 1233, 1479, 2540, 2908
Hpy99I CGWCG 1 cut(s) 2172
HpyAV CCTTC 9 cut(s) 434, 502, 604, 864, 912, 1640, 1777, 2476, 3096
HpyCH4IV ACGT 3 cut(s) 803, 1288, 2766
HpyF10VI GCNNNNNNNGC 7 cut(s) 1109, 1118, 1426, 1435, 2229, 3046, 3107
HpyF3I CTNAG 7 cut(s) 240, 477, 2117, 2703, 2795, 2991, 3111
HpySE526I ACGT 3 cut(s) 803, 1288, 2766
Hsp92I GRCGYC 1 cut(s) 1288
HspAI GCGC 2 cut(s) 1726, 2342
Kpn2I TCCGGA 1 cut(s) 2190
KpnI GGTACC 1 cut(s) 2413
Ksp22I TGATCA 1 cut(s) 655
LguI GCTCTTC 1 cut(s) 2351
LmnI GCTCC 4 cut(s) 36, 815, 1812, 2869
Lsp1109I GCAGC 7 cut(s) 1005, 1223, 1255, 1438, 1483, 1816, 1910
LweI GCATC 4 cut(s) 274, 1043, 1383, 1400
MaeI CTAG 9 cut(s) 668, 1157, 1421, 1916, 2291, 2628, 2667, 2708, 3141
MaeII ACGT 3 cut(s) 803, 1288, 2766
MaeIII GTNAC 5 cut(s) 130, 928, 2326, 2956, 3005
MbiI CCGCTC 1 cut(s) 2794
MflI RGATCY 4 cut(s) 1184, 1663, 1950, 1969
MhlI GDGCHC 3 cut(s) 69, 397, 2866
MlyI GAGTC 5 cut(s) 1646, 1762, 2194, 2360, 3019
MmeI TCCRAC 2 cut(s) 1513, 2072
MroI TCCGGA 1 cut(s) 2190
MroXI GAANNNNTTC 4 cut(s) 1448, 1590, 1850, 2359
MslI CAYNNNNRTG 2 cut(s) 2032, 2847
MspA1I CMGCKG 4 cut(s) 1133, 1239, 1496, 2149
MspI CCGG 6 cut(s) 468, 574, 980, 2191, 2954, 3123
MspR9I CCNGG 9 cut(s) 93, 468, 574, 575, 598, 980, 981, 1508, 2210
Mva1269I GAATGC 1 cut(s) 2316
MvaI CCWGG 4 cut(s) 93, 598, 1508, 2210
MwoI GCNNNNNNNGC 7 cut(s) 1109, 1118, 1426, 1435, 2229, 3046, 3107
NciI CCSGG 5 cut(s) 468, 574, 575, 980, 981
NcoI CCATGG 1 cut(s) 3096
NdeI CATATG 1 cut(s) 874
NlaIV GGNNCC 4 cut(s) 1005, 1537, 1714, 2411
NmuCI GTSAC 1 cut(s) 2326
NspI RCATGY 3 cut(s) 18, 843, 1901
NspV TTCGAA 1 cut(s) 99
PasI CCCWGGG 1 cut(s) 597
PceI AGGCCT 1 cut(s) 1523
PciI ACATGT 1 cut(s) 1897
PciSI GCTCTTC 1 cut(s) 2351
PctI GAATGC 1 cut(s) 2316
PdmI GAANNNNTTC 4 cut(s) 1448, 1590, 1850, 2359
PflMI CCANNNNNTGG 1 cut(s) 413
PfoI TCCNGGA 1 cut(s) 1506
PkrI GCNGC 8 cut(s) 995, 1177, 1213, 1245, 1428, 1498, 1806, 1925
PleI GAGTC 5 cut(s) 1645, 1762, 2194, 2360, 3019
PpsI GAGTC 5 cut(s) 1645, 1762, 2194, 2360, 3019
PpuMI RGGWCCY 1 cut(s) 899
PscI ACATGT 1 cut(s) 1897
PshBI ATTAAT 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 2866
Psp1406I AACGTT 1 cut(s) 2766
Psp5II RGGWCCY 1 cut(s) 899
Psp6I CCWGG 4 cut(s) 91, 596, 1506, 2208
PspFI CCCAGC 2 cut(s) 1000, 1979
PspGI CCWGG 4 cut(s) 91, 596, 1506, 2208
PspN4I GGNNCC 4 cut(s) 1005, 1537, 1714, 2411
PspPI GGNCC 4 cut(s) 465, 899, 1004, 1712
PspPPI RGGWCCY 1 cut(s) 899
PstI CTGCAG 1 cut(s) 1928
PsuI RGATCY 4 cut(s) 1184, 1663, 1950, 1969
PvuII CAGCTG 2 cut(s) 1133, 1496
RseI CAYNNNNRTG 2 cut(s) 2032, 2847
SacI GAGCTC 1 cut(s) 2866
SapI GCTCTTC 1 cut(s) 2351
SatI GCNGC 8 cut(s) 994, 1176, 1212, 1244, 1427, 1497, 1805, 1924
Sau96I GGNCC 4 cut(s) 465, 899, 1004, 1712
ScaI AGTACT 1 cut(s) 758
SchI GAGTC 5 cut(s) 1646, 1762, 2194, 2360, 3019
ScrFI CCNGG 9 cut(s) 93, 468, 574, 575, 598, 980, 981, 1508, 2210
SduI GDGCHC 3 cut(s) 69, 397, 2866
SfaNI GCATC 4 cut(s) 274, 1043, 1383, 1400
SfcI CTRYAG 4 cut(s) 1431, 1471, 1924, 2656
SfuI TTCGAA 1 cut(s) 99
SinI GGWCC 3 cut(s) 899, 1004, 1712
SmaI CCCGGG 2 cut(s) 575, 981
SmiMI CAYNNNNRTG 2 cut(s) 2032, 2847
SmlI CTYRAG 3 cut(s) 1973, 2393, 2525
SmoI CTYRAG 3 cut(s) 1973, 2393, 2525
SpeI ACTAGT 1 cut(s) 667
SseBI AGGCCT 1 cut(s) 1523
SsiI CCGC 9 cut(s) 425, 798, 1101, 1154, 1175, 1239, 1824, 2149, 2792
SspMI CTAG 9 cut(s) 668, 1157, 1421, 1916, 2291, 2628, 2667, 2708, 3141
SstI GAGCTC 1 cut(s) 2866
StuI AGGCCT 1 cut(s) 1523
StyD4I CCNGG 9 cut(s) 91, 466, 572, 573, 596, 978, 979, 1506, 2208
StyI CCWWGG 4 cut(s) 496, 715, 1264, 3096
TaiI ACGT 3 cut(s) 806, 1291, 2769
TaqII GACCGA 1 cut(s) 2711
TatI WGTACW 5 cut(s) 557, 756, 1314, 2105, 2136
TauI GCSGC 1 cut(s) 1178
TseFI GTSAC 1 cut(s) 2326
TseI GCWGC 7 cut(s) 993, 1211, 1243, 1426, 1496, 1804, 1923
Tsp45I GTSAC 1 cut(s) 2326
TspDTI ATGAA 9 cut(s) 20, 171, 357, 453, 665, 768, 1829, 2097, 2603
TspGWI ACGGA 3 cut(s) 837, 2053, 2933
TspMI CCCGGG 2 cut(s) 573, 979
Van91I CCANNNNNTGG 1 cut(s) 413
VneI GTGCAC 1 cut(s) 393
VpaK11BI GGWCC 3 cut(s) 899, 1004, 1712
VspI ATTAAT 1 cut(s) 342
XceI RCATGY 3 cut(s) 18, 843, 1901
XmaI CCCGGG 2 cut(s) 573, 979
XmiI GTMKAC 1 cut(s) 1232
XmnI GAANNNNTTC 4 cut(s) 1448, 1590, 1850, 2359
XspI CTAG 9 cut(s) 668, 1157, 1421, 1916, 2291, 2628, 2667, 2708, 3141
ZraI GACGTC 1 cut(s) 1289
ZrmI AGTACT 1 cut(s) 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.